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https://www.readbyqxmd.com/read/30326434/integrative-analysis-of-pectin-methylesterase-pme-and-pme-inhibitors-in-tomato-solanum-lycopersicum-identification-tissue-specific-expression-and-biochemical-characterization
#1
Ho Young Jeong, Hong Phuong Nguyen, Seok Hyun Eom, Chanhui Lee
Although previous studies have demonstrated that the degree of demethylesterification of pectin polysaccharides is modulated during tomato fruit ripening, its involvement in vegetative organ development has been seldom investigated. As a first step in understanding the importance of pectin modification during vegetative stages, we used chemical, biochemical, and molecular approaches to analyze PMEs and PMEIs in tomato plants. We found that tomato cell walls isolated from vegetative tissues as well as the fruit contain substantial quantities of pectin, and different degrees of methylesterification were evident in different tissues...
October 9, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/30326357/spermatogenesis-dysfunction-induced-by-pm-2-5-from-automobile-exhaust-via-the-ros-mediated-mapk-signaling-pathway
#2
Bin Liu, Sheng-De Wu, Lian-Ju Shen, Tian-Xin Zhao, Yi Wei, Xiang-Liang Tang, Chun-Lan Long, Yue Zhou, Da-Wei He, Tao Lin, Guang-Hui Wei
Long-term exposure to particulate matter 2.5 (PM2.5 ) from automobile exhaust impairs spermatogenesis through oxidative stress injury, but the underlying mechanism is unknown. To investigate the toxic mechanism of PM2.5 -induced spermatogenesis impairment, we focused on the MAPK signaling pathway. We also examined the effects of treatment with vitamins C and E on spermatogenic function. Male SD rats were divided randomly into three groups: control (0.9% sterilized saline), PM2.5 exposure (20 mg/kg.b.w.), and PM2...
October 13, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/30326351/single-incision-lateral-release-for-hallux-valgus-using-a-cervical-biopsy-blade-a-cadaveric-study
#3
James Kennedy, Andrew Berg, Luke Welsh, Jaysheel Mehta
The role of lateral release is widely accepted and regarded as a key step in the management of hallux valgus. There remains however debate in the literature in regards to which structures should be divided and the method of approach. As such, a technique of lateral release using a curved blade placed inferior to the metatarsal head to divide the lateral metatarsosesamoid suspensory ligament and incise the lateral joint capsule has been developed. This technique was performed on fourteen fresh frozen cadaveric specimens which were then dissected...
May 1, 2018: Foot
https://www.readbyqxmd.com/read/30326324/within-tester-repeatability-and-between-tester-reproducibility-of-skin-test-endpoint-titration-a-quality-assurance-study
#4
Christianne M Blais, Beth E Davis, Donald W Cockcroft
No abstract text is available yet for this article.
October 13, 2018: Annals of Allergy, Asthma & Immunology
https://www.readbyqxmd.com/read/30326293/enlarged-perivascular-spaces-in-brain-mri-automated-quantification-in-four-regions
#5
Florian Dubost, Pinar Yilmaz, Hieab Adams, Gerda Bortsova, M Arfan Ikram, Wiro Niessen, Meike Vernooij, Marleen de Bruijne
Enlarged perivascular spaces (PVS) are structural brain changes visible in MRI, are common in aging, and are considered a reflection of cerebral small vessel disease. As such, assessing the burden of PVS has promise as a brain imaging marker. Visual and manual scoring of PVS is a tedious and observer-dependent task. Automated methods would advance research into the etiology of PVS, could aid to assess what a "normal" burden is in aging, and could evaluate the potential of PVS as a biomarker of cerebral small vessel disease...
October 13, 2018: NeuroImage
https://www.readbyqxmd.com/read/30326270/the-effects-of-stress-cortisol-administration-and-cortisol-inhibition-on-black-sea-bass-centropristis-striata-sex-differentiation
#6
Kelsie A Miller, Linas W Kenter, Timothy S Breton, David L Berlinsky
Sex differentiation in many lower vertebrates (e.g. reptiles, amphibians, and fishes) can be influenced by environmental factors experienced during sensitive developmental periods. Environmental stressors, acting through cortisol, masculinize some teleost fishes during development by limiting gonadal cytochrome P450 aromatase (cyp19a1a), the enzyme that irreversibly converts testosterone to 17β-estradiol. In this study, we examined the influence of cortisol, cortisol inhibitors and a repeated, acute stressor (net-chasing) on sex differentiation in black sea bass (BSB; Centropristis striata), a protogynous hermaphroditic teleost...
October 13, 2018: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/30326259/phosphorylation-of-nherf1-s279-and-s301-differentially-regulates-breast-cancer-cell-phenotype-and-metastatic-organotropism
#7
Maria Raffaella Greco, Emeline Bon, Rosa Rubino, Lorenzo Guerra, Manuel Bernabe-Garcia, Stefania Cannone, Maria-Luisa Cayuela, Loredana Ciaccia, Séverine Marionneau-Lambot, Thibauld Oullier, Gaëlle Fromont, Roseline Guibon, Sébastien Roger, Stephan Joel Reshkin, Rosa Angela Cardone
Metastatic cancer cells are highly plastic for the expression of different tumor phenotype hallmarks and organotropism. This plasticity is highly regulated but the dynamics of the signaling processes orchestrating the shift from one cell phenotype and metastatic organ pattern to another are still largely unknown. The scaffolding protein NHERF1 has been shown to regulate the expression of different neoplastic phenotypes through its PDZ domains, which forms the mechanistic basis for metastatic organotropism. This reprogramming activity was postulated to be dependent on its differential phosphorylation patterns...
October 13, 2018: Biochimica et biophysica acta. Molecular basis of disease
https://www.readbyqxmd.com/read/30326209/reproducibility-of-the-measurement-of-bulk-tapped-density-of-pharmaceutical-powders-between-pharmaceutical-laboratories
#8
Ilgaz Akseli, Jon Hilden, Jeffrey M Katz, Ron C Kelly, Timothy T Kramer, Chen Mao, Frederick Osei-Yeboah, John C Strong
The bulk properties of a powder are dependent upon the preparation, treatment and storage of the sample, i.e. how it was handled. The particles can be packed to have a range of bulk densities and, moreover, the slightest disturbance of the powder bed may result in a changed bulk density. Thus, the bulk density of a powder is often difficult to measure with good reproducibility and, in reporting the results, it is essential to specify how the determination was made. In this paper, we measured the bulk density, tapped density and calculated the Hausner Ratio of commonly used excipients with similar tapped density testers and followed the USP30-NF25-S1 testing procedure...
October 13, 2018: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/30326202/an-open-source-automated-surgical-instrument-for-microendoscope-implantation
#9
Bo Liang, Lifeng Zhang, Casey Moffitt, Yun Li, Da-Ting Lin
BACKGROUND: Gradient index (GRIN) lenses can be used to image deep brain regions otherwise inaccessiblevia standard optical imaging methods. Brain tissue aspiration before GRIN lens implantation is a widely adopted approach. However, typical brain tissue aspiration methods still rely on a handheld vacuum needle, which is subjective to human error and low reproducibility. Therefore, a high-precision automated surgical instrument for brain tissue aspiration is desirable. NEW METHOD: We developed a robotic surgical instrument that utilizes robotic control of a needle connected to a vacuum pump to aspirate brain tissue...
October 13, 2018: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/30326138/mccarus-minimally-invasive-hysterectomy-20-years-experience-lessons-learned
#10
Steven D McCarus
The advancement of surgical innovation for both devices and techniques has directly impacted the number of hysterectomy options available to patients. These advancements have led to an expansion of options that has been exceptionally impactful for minimally invasive surgery. For individuals who are diagnosed with a health condition or disease that requires a hysterectomy, these advances allow the surgeon to consider an expanded variety of procedures that may improve patients' outcomes and accommodate patient preferences...
October 16, 2018: Surgical Technology International
https://www.readbyqxmd.com/read/30326007/association-of-unintended-pregnancy-with-spontaneous-fetal-loss-in-women-with-epilepsy-findings-of-the-epilepsy-birth-control-registry
#11
Andrew G Herzog, Hannah B Mandle, Devon B MacEachern
Importance: If unintended pregnancy is common among women with epilepsy and is associated with increased risk of spontaneous fetal loss (SFL), it is important to develop guidelines for safe and effective contraception for this community. Objective: To assess whether planned pregnancy is a determinant of SFL in women with epilepsy. Design, Setting, and Participants: The Epilepsy Birth Control Registry conducted this web-based, retrospective survey between 2010 and 2014...
October 15, 2018: JAMA Neurology
https://www.readbyqxmd.com/read/30325972/in-utero-exposure-to-thermal-stress-has-long-term-effects-on-mammary-gland-microstructure-and-function-in-dairy-cattle
#12
Amy L Skibiel, Bethany Dado-Senn, Thiago F Fabris, Geoffrey E Dahl, Jimena Laporta
Earth's rising temperature has substantial repercussions for food-producing animals by increasing morbidity and mortality, diminishing reproductive potential, and reducing productivity. In the dairy industry this equates to massive losses in milk yield, which occur when cows are exposed to heat stress during lactation or during the non-lactating period between lactations (i.e. dry period). Furthermore, milk yield is significantly lower in first-lactation heifers that experienced fetal heat stress. The mechanisms underlying intrauterine effects of heat stress on the offspring's future lactation have yet to be fully elucidated...
2018: PloS One
https://www.readbyqxmd.com/read/30325932/persistently-high-incidence-of-hiv-and-poor-service-uptake-in-adolescent-girls-and-young-women-in-rural-kwazulu-natal-south-africa-prior-to-dreams
#13
Natsayi Chimbindi, Nondumiso Mthiyane, Isolde Birdthistle, Sian Floyd, Nuala McGrath, Deenan Pillay, Janet Seeley, Thembelihle Zuma, Jaco Dreyer, Dickman Gareta, Tinofa Mutevedzi, Justin Fenty, Kobus Herbst, Theresa Smit, Kathy Baisley, Maryam Shahmanesh
BACKGROUND: Adolescent girls and young women (AGYW) bear the brunt of the HIV epidemic in South Africa. 'DREAMS' aims to reduce HIV incidence through multi-level combination prevention. We describe HIV incidence and uptake of HIV and sexual reproductive health (SRH) by AGYW in KwaZulu-Natal (KZN), prior to DREAMS. METHODS: Longitudinal and cross-sectional analysis of women (15-24 year old) in a population-based HIV incidence cohort within a demographic surveillance site in KZN...
2018: PloS One
https://www.readbyqxmd.com/read/30325922/oxygenation-in-cell-culture-critical-parameters-for-reproducibility-are-routinely-not-reported
#14
Abdullah Al-Ani, Derek Toms, Douglas Kondro, Jarin Thundathil, Yang Yu, Mark Ungrin
Mammalian cell culture is foundational to biomedical research, and the reproducibility of research findings across the sciences is drawing increasing attention. While many components contribute to reproducibility, the reporting of factors that impact oxygen delivery in the general biomedical literature has the potential for both significant impact, and immediate improvement. The relationship between the oxygen consumption rate of cells and the diffusive delivery of oxygen through the overlying medium layer means parameters such as medium depth and cell type can cause significant differences in oxygenation for cultures nominally maintained under the same conditions...
2018: PloS One
https://www.readbyqxmd.com/read/30325901/cytokines-and-endometrial-receptivity-after-intracytoplasmic-sperm-injection-a-cohort-study-at-islamabad
#15
Rehana Rehman, Mussarat Ashraf, Anila Jasmine, Karishma Lal, Faiza Alam
OBJECTIVE: To evaluate the role of tumour necrosis factor alpha and interleukin-6 in conception after assisted reproductive treatment. METHODS: The longitudinal cohort study was conducted from August 2014 to May 2015 and comprised patients undergoing intracytoplasmic sperm injection at the Islamabad Clinic Serving Infertile Couples, Islamabad, Pakistan. During the procedure, endometrial thickness was measured on the day of ovulation induction and serum tumour necrosis factor alpha along with interleukin-6 were measured on the embryo transfer day...
June 2018: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/30325835/single-surgeon-experience-for-maximizing-outcomes-in-implant-based-breast-reconstruction-in-chinese-patients
#16
Shanshan He, Jian Yin, Jingyan Sun, Qingfeng Huang, Jing Liu, Chunyong Han, Zhuming Yin
INTRODUCTION: Breast reconstruction for Chinese patients is vastly different given cultural differences, patient preferences, access to resources, and insurance coverage in China. Given these unique factors, a different approach for optimizing outcomes should be considered. METHODS: Retrospective review of all patients undergoing implant-based breast reconstruction from January 2013 to May 2016 was performed. Esthetic evaluations were made both by the patients and 1 nonoperative surgeon at least 6 months postoperative, and patient satisfaction was assessed using the Breast-Q...
October 15, 2018: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/30325761/an-evaluation-of-the-iolmaster-700
#17
Mark A Bullimore, Stephen Slade, Paul Yoo, Todd Otani
OBJECTIVES: To evaluate the repeatability and reproducibility of the Carl Zeiss Meditec IOLMaster 700, which uses swept-source optical coherence tomography technology, along with its agreement with the IOLMaster 500 and Lenstar LS 900. METHODS: In a clinical practice, complete measurements were taken on one eye of 100 subjects: 51 with cataracts and 49 with clear lenses. Three sets of measurements were taken by 3 operators with three different IOLMaster 700 units, and one operator took 3 measurements with the IOLMaster 500 and the Lenstar...
October 15, 2018: Eye & Contact Lens
https://www.readbyqxmd.com/read/30325683/evaluation-and-characteristics-of-immunological-adjuvant-activity-of-purified-fraction-of-albizia-julibrissin-saponins
#18
Binnian Zhu, Tianyu He, Xiangyun Gao, Minghua Shi, Hongxiang Sun
PURPOSE: The purified fraction of Albizia julibrissin saponins (AJSAF) was evaluated and characterized for the adjuvant activity on porcine reproductive and respiratory syndrome virus (PRRSV) vaccine. METHODS: The effects of AJSAF on serum PRRSV N protein-specific antibody titers, splenocyte proliferation, natural killer (NK) cell activity, mRNA expression of cytokines and transcription factors, secretion of cytokines, T cells response in splenocytes, as well as delayed type hypersensitivity (DTH) in the mice immunized PRRSV vaccine were determined by ELISA, MTT assay, flow cytometry and quantitative real-time PCR (qRT-PCR)...
October 16, 2018: Immunological Investigations
https://www.readbyqxmd.com/read/30325674/macrophage-reprogramming-by-negatively-charged-membrane-phospholipids-controls-infection
#19
David M Cauvi, Dennis Hawisher, Paulo R Dores-Silva, Radhames E Lizardo, Antonio De Maio
Extracellular vesicles (ECVs) are heterogeneous membrane-enclosed structures containing proteins, nucleic acids, and lipids that participate in intercellular communication by transferring their contents to recipient cells. Although most of the attention has been directed at the biologic effect of proteins and microRNA, the contribution of phospholipids present in ECVs on cellular activation has not been extensively addressed. We investigated the biologic effect of phosphatidylserine (PS) and phosphatidylcholine (PC), 2 phospholipids highly abundant in ECVs...
October 16, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30325671/conserving-and-sharing-taro-genetic-resources-for-the-benefit-of-global-taro-cultivation-a-core-contribution-of-the-centre-for-pacific-crops-and-trees
#20
Andreas W Ebert, Logotonu M Waqainabete
This review article gives an account of the origin, domestication, and dispersal of taro, a staple food crop in many countries in the humid tropics and subtropics. Genetic diversity studies indicated that distinct gene pools exist in all the regions where taro may be naturally distributed-the Indian subcontinent, China, Southeast Asia, and in Oceania. The Asian gene pool presented the highest genetic diversity. Diploid taro is prevalent in the Pacific Islands, while both diploids and triploids are found in mainland Asia...
October 2018: Biopreservation and Biobanking
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