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https://www.readbyqxmd.com/read/30120959/efficacy-of-low-temperature-plasma-activated-gas-disinfection-against-biofilm-on-contaminated-gi-endoscope-channels
#1
Sudhir Bhatt, Poonam Mehta, Chen Chen, Christina L Schneider, Lauren N White, Hai-Lan Chen, Michael G Kong
BACKGROUND: It has been increasingly recognized that the safety of gastrointestinal endoscopes needs to be improved by addressing the small margin of safety of high-level disinfectants (HLD), and the failure of HLD to clear multi-drug resistant organisms and biofilms. There is also an unmet need for effective low-temperature sterilization techniques that have a clear pathway for FDA clearance. Here, we report the results of our investigation of a novel argon plasma-activated gas (PAG) for disinfection and potentially sterilization of biofilm-contaminated endoscopic channels...
August 15, 2018: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/30120946/impact-of-low-dose-chronic-exposure-to-bisphenol-a-and-its-analogue-bisphenol-b-bisphenol-f-and-bisphenol-s-on-hypothalamo-pituitary-testicular-activities-in-adult-rats-a-focus-on-the-possible-hormonal-mode-of-action
#2
Asad Ullah, Madeeha Pirzada, Sarwat Jahan, Hizb Ullah, Naheed Turi, Waheed Ullah, Mariyam Fatima Siddiqui, Muhammad Zakria, Kinza Zafar Lodhi, Muhammad Munir Khan
Bisphenol A an estrogen-mimic endocrine disrupting chemical, used to manufacture polycarbonate plastics and epoxy resins with toxic effects for male reproduction. Due to its toxicity, industries have started to replace it with other bisphenols. In this study, the toxicity of BPA analogues (BPB, BPF and BPS) was evaluated in a chronic study. We investigated whether the chronic exposure to low bisphenols doses affects spermatogenesis with outcomes on oxidative stress and male reproductive system. Male rats (22 day old) were exposed to water containing 0...
August 15, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/30120870/short-and-long-term-outcomes-in-children-born-after-assisted-reproductive-technologies-art
#3
Anja Pinborg
It has been forty years since the first IVF child, Louise Brown, born in 1978 and now more than 7 million children are conceived after assisted reproductive technologies (ART). There has been a tremendous increase in the number of ART cycles and several more advanced treatments have been developed, such as intracytoplasmic sperm injection (ICSI). ICSI revolutionized the treatment of severe male factor infertility after the first ICSI baby born in 1992. This article is protected by copyright. All rights reserved...
August 18, 2018: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/30120856/use-of-vhh-antibodies-for-development-of-antigen-detection-test-for-visceral-leishmaniasis
#4
Claudia Abeijon, Julia Dilo, Jacqueline M Tremblay, Agostinho G Viana, Lilian L Bueno, Silvio F G Carvalho, Ricardo T Fujiwara, Charles B Shoemaker, Antonio Campos-Neto
We have recently developed a sensitive and specific urine-based antigen detection ELISA for the diagnosis of visceral leishmaniasis (VL). This assay used rabbit IgG and chicken IgY polyclonal antibodies specific for the Leishmania infantum proteins iron superoxide dismutase 1 (Li-isd1), tryparedoxin1 (Li-txn1), and nuclear transport factor 2 (Li-ntf2). However, polyclonal antibodies have limitations for upscaling and continuous supply. To circumvent these hurdles, we began to develop immortalized monoclonal antibodies...
August 18, 2018: Parasite Immunology
https://www.readbyqxmd.com/read/30120840/expression-and-localization-of-follicle-stimulating-hormone-receptor-in-the-yak-uterus-during-different-stages-of-the-estrous-cycle
#5
Ayimuguli Abudureyimu, Yong Cai, Shengdong Huo, Rui Ren, Rongxin Zang, Jutian Yang, Zhongren Ma, Yan Cui
Morphological changes of the uterus and alterations in its secretory activity under the influence of steroid hormones been well documented. The estrous cycle is also associated with significant changes in plasma follicle stimulating hormone (FSH), whose effects are mediated through its receptor (FSHR). Reports showed that in many mammals, FSHR were expressed in gonadal and extragonadal tissues including cervix, female reproductive tract, pituitary gland, etc. Follicle stimulating hormone (FSH) signals through endothelial FSHR directly stimulate angiogenesis and involved in the timing of birth in human, and the presence of FSHR in the placenta is essential for normal pregnance in mice...
August 18, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/30120839/development-of-a-methodology-to-combine-fmri-and-emg-to-measure-emotional-responses-in-patients-with-anorexia-nervosa
#6
Tom Hildebrandt, Kurt Schulz, Lazar Fleysher, Trevor Griffen, Ashley Heywood, Robyn Sysko
OBJECTIVE: Individuals with eating disorders are theorized to have basic impairments in affective appraisal and social-emotional processing that contribute to pathogenesis of the disease. We aimed to determine if facial electromyography could be used to discriminate between happy and disgust emotions during simultaneous acquisition of an fMRI BOLD sequence in efforts to establish a novel tool for investigating emotion-driven hypotheses about eating pathology. In line with standards for rigor and reproducibility, we provide detailed protocols and code to support each step of this project...
August 18, 2018: International Journal of Eating Disorders
https://www.readbyqxmd.com/read/30120801/local-production-of-prolactin-in-lesions-may-play-a-pathogenic-role-in-psoriatic-patients-and-imiquimod-induced-psoriasis-like-mouse-model
#7
Hui Yang, Xia Li, Feng Xue, Qunli Xia, Xin Zhao, Dixin Wang, Lihong Chen, Hua Cao, Han Xu, Xiaoyan Shen, Weiru Yuan, Xiaoqing Zhao, Ruofei Shi, Jie Zheng
Human prolactin (PRL) is a well-known hormone for pituitary of lactation and reproduction, but it also has immunostimulatory effect in some inflammatory or autoimmune diseases including psoriasis, which has not been well elucidated. This study aimed to determine the relationship between PRL and psoriasis through clinical case-control studies, and explore the function of PRL in the pathogenesis of imiquimod (IMQ)-induced psoriasis-like mouse model. Serum from patients with psoriasis vulgaris (PsV), patients with erythrodermic psoriasis, and healthy controls (HCs) were collected for PRL test...
August 18, 2018: Experimental Dermatology
https://www.readbyqxmd.com/read/30120797/the-evidential-statistical-paradigm-in-genetics
#8
REVIEW
Lisa J Strug
Concerns over reproducibility in research has reinvigorated the discourse on P-values as measures of statistical evidence. In a position statement by the American Statistical Association board of directors, they warn of P-value misuse and refer to the availability of alternatives. Despite the common practice of comparing P-values across different hypothesis tests in genetics, it is well-appreciated that P-values must be interpreted alongside the sample size and experimental design used for their computation...
August 18, 2018: Genetic Epidemiology
https://www.readbyqxmd.com/read/30120788/a-novel-enzymatic-method-for-the-measurement-of-lactose-in-lactose-free-products
#9
D Mangan, B V McCleary, H Culleton, C Cornaggia, R Ivory, V McKie, E Delaney, T Kargelis
BACKGROUND: In recent years there has been a surge in the number of commercially available lactose-free variants of a wide variety of products. This presents an analytical challenge for the measurement of the residual lactose content in the presence of high levels of mono-, di-, and oligosaccharides. RESULTS: In the current work, we describe the development of a novel enzymatic low-lactose determination method termed LOLAC which is based on an optimised glucose removal pre-treatment step followed by a sequential enzymatic assay which measures residual glucose and lactose in a single cuvette...
August 17, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/30120771/condition-analysis-of-the-brazilian-sharpnose-shark-rhizoprionodon-lalandii-evidence-of-maternal-investment-for-initial-post-natal-life
#10
J T Corsso, O B F Gadig, R R P Barreto, F S Motta
The present study analysed the condition factor (K) and the hepato-somatic index (IH ) of the Brazilian sharpnose shark Rhizoprionodon lalandii during its entire life cycle. Discontinuity of liver growth was observed after sexual maturity. High condition values were recorded in the length classes of 300-350 mm (neonates), when the livers represented about 8% of total mass, decreasing until the length classes of 400-450 mm and 450-500 mm in females and males, respectively. Seasonal analysis exhibited low IH values in the summer, while for K, low values were in spring, indicating greater investment for body growth during spring and for gonad maturation in summer...
August 17, 2018: Journal of Fish Biology
https://www.readbyqxmd.com/read/30120614/establishment-and-evaluation-of-a-general-dissociation-technique-for-antibodies-in-circulating-immune-complexes
#11
Tong Wang, Meng Zhang, Huajun Zhou, Dawei Cui, Xujian Xu, Changgui Sun, Yuzhu Dai, Jun Cheng
This study aimed to establish a general and efficient dissociation technique for detecting antibodies in circulating immune complexes (CICs) in serum and to evaluate its clinical application. CICs were efficiently separated from specimens using polyethylene glycol double-precipitation. The best conditions for anti-HBs dissociation from HBsAg-ICs were a pH of 1.80, incubation at 15 °C for 5-10 min, and detection within 10 min after neutralization. The mean dissociation rate, reproducibility, mean dissociation recovery rate and specificity of the new technique were 64...
August 17, 2018: Clinical and Experimental Medicine
https://www.readbyqxmd.com/read/30120595/practical-guidelines-for-rigor-and-reproducibility-in-preclinical-and-clinical-studies-on-cardioprotection
#12
REVIEW
Hans Erik Bøtker, Derek Hausenloy, Ioanna Andreadou, Salvatore Antonucci, Kerstin Boengler, Sean M Davidson, Soni Deshwal, Yvan Devaux, Fabio Di Lisa, Moises Di Sante, Panagiotis Efentakis, Saveria Femminò, David García-Dorado, Zoltán Giricz, Borja Ibanez, Efstathios Iliodromitis, Nina Kaludercic, Petra Kleinbongard, Markus Neuhäuser, Michel Ovize, Pasquale Pagliaro, Michael Rahbek-Schmidt, Marisol Ruiz-Meana, Klaus-Dieter Schlüter, Rainer Schulz, Andreas Skyschally, Catherine Wilder, Derek M Yellon, Peter Ferdinandy, Gerd Heusch
No abstract text is available yet for this article.
August 17, 2018: Basic Research in Cardiology
https://www.readbyqxmd.com/read/30120575/a-hollow-microporous-organic-network-as-a-fiber-coating-for-solid-phase-microextraction-of-short-chain-chlorinated-hydrocarbons
#13
Jingkun Li, Huijuan Li, Yanfang Zhao, Shanshan Wang, Xiangfeng Chen, Ru-Song Zhao
A solid-phase microextraction (SPME) fiber coated with a hollow microporous organic network (H-MON) was fabricated for the analysis of short-chain chlorinated paraffins (SCCPs). The sorbent was prepared by reacting tetra(4-ethynylphenyl)methane and 1,4-diiodobenzene using bis-(triphenylphosphine) palladium(II) as the catalyst in the presence of silica sphere templates, which then were removed by hydrofluoric acid. The SCCPs were quantified by GC-MS working in the negative chemical ionization mode. The H-MON has a high specific surface (701 m2 ·g-1 ) and microporosity (pore size <2 nm)...
August 17, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/30120546/is-myotomy-plus-diverticulopexy-suitable-for-symptomatic-zenker-s-diverticula
#14
Jacopo Vannucci, Alberto Matricardi, Elisa Scarnecchia, Rosanna Capozzi, Valeria Liparulo, Stefano Santoprete, Lucio Cagini, Francesco Puma
The aim of the study was to prospectively evaluate the outcome of myotomy plus diverticulopexy over short and long-terms. A prospectively collected consecutive series (2007-2017) of 37 patients undergoing myotomy plus diverticulopexy was analyzed for clinical condition, operative information, peri-operative events, and follow-up by means of interview and physical examination. Diverticulopexy was scheduled regardless of the diverticulum's features and patient condition, other than operability. There was no choice or selection between possible treatment options...
August 17, 2018: Dysphagia
https://www.readbyqxmd.com/read/30120331/simple-rules-for-construction-of-a-geometric-nest-structure-by-pufferfish
#15
Ryo Mizuuchi, Hiroshi Kawase, Hirofumi Shin, Daisuke Iwai, Shigeru Kondo
A small (~10 cm) male pufferfish (Torquigener albomaculosus) builds a large (~2 m) sandy nest structure, resembling a mysterious crop circle, to attract females. The circle consists of radially arranged deep ditches in the outer ring region, and maze-like shallow ditches in the central region. The configuration is geometrical. Here, we examined the process of the outer ring construction, and extracted the 'rules' followed by the pufferfish. During construction, the pufferfish repeatedly excavates ditches from the outside in...
August 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30120304/social-bees-are-fitter-in-more-biodiverse-environments
#16
Benjamin F Kaluza, Helen M Wallace, Tim A Heard, Vanessa Minden, Alexandra Klein, Sara D Leonhardt
Bee population declines are often linked to human impacts, especially habitat and biodiversity loss, but empirical evidence is lacking. To clarify the link between biodiversity loss and bee decline, we examined how floral diversity affects (reproductive) fitness and population growth of a social stingless bee. For the first time, we related available resource diversity and abundance to resource (quality and quantity) intake and colony reproduction, over more than two years. Our results reveal plant diversity as key driver of bee fitness...
August 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30120295/characterization-of-induced-tissue-specific-stem-cells-from-pancreas-by-a-synthetic-self-replicative-rna
#17
Chika Miyagi-Shiohira, Yoshiki Nakashima, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi
Induced pluripotent stem (iPS) cells have significant implications for overcoming most of the ethical issues associated with embryonic stem (ES) cells. Furthermore, our recent study demonstrated the generation of induced tissue-specific stem (iTS) cells by transient overexpression of the reprogramming factors using a plasmid combined with tissue-specific selection. In this study, we were able to generate RNA-based iTS cells that utilize a single, synthetic, self-replicating VEE-RF RNA replicon expressing four reprogramming factors (OCT4, KLF4, SOX2, and GLIS1)...
August 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30120288/predictive-ability-of-genome-assisted-statistical-models-under-various-forms-of-gene-action
#18
Mehdi Momen, Ahmad Ayatollahi Mehrgardi, Ayyub Sheikhi, Andreas Kranis, Llibertat Tusell, Gota Morota, Guilherme J M Rosa, Daniel Gianola
Recent work has suggested that the performance of prediction models for complex traits may depend on the architecture of the target traits. Here we compared several prediction models with respect to their ability of predicting phenotypes under various statistical architectures of gene action: (1) purely additive, (2) additive and dominance, (3) additive, dominance, and two-locus epistasis, and (4) purely epistatic settings. Simulation and a real chicken dataset were used. Fourteen prediction models were compared: BayesA, BayesB, BayesC, Bayesian LASSO, Bayesian ridge regression, elastic net, genomic best linear unbiased prediction, a Gaussian process, LASSO, random forests, reproducing kernel Hilbert spaces regression, ridge regression (best linear unbiased prediction), relevance vector machines, and support vector machines...
August 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30120012/associations-between-serum-calcium-concentration-and-postpartum-health-and-reproductive-performance-in-dairy-cows
#19
Jae Kwan Jeong, Hyun Gu Kang, Ill Hwa Kim
In the present study, the aim was to evaluate the associations between serum calcium concentration after calving and serum metabolites, postpartum disorders, and reproductive performance in dairy cows. Blood samples were collected from 200 Holstein cows immediately and 1, 2, 4, 6, and 8 weeks after calving to measure serum metabolites and progesterone concentrations. Cows were divided into three groups on the basis of the mean serum calcium concentration after calving: a Low group (<7.9 mg/dL, n = 52), a Moderate group (7...
August 8, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/30119971/enhanced-magnetic-ionic-liquid-based-dispersive-liquid-liquid-microextraction-of-triazines-and-sulfonamides-through-a-one-pot-ph-modulated-approach
#20
Theodoros G Chatzimitakos, Stephen A Pierson, Jared L Anderson, Constantine D Stalikas
In this study, an enhanced variant of magnetic ionic liquid (MIL)-based dispersive liquid-liquid microextraction is put forward. The procedure combines a water insoluble solid support and the [P66614 + ][Dy(III)(hfacac)4 - ] MIL, in a one-pot, pH-modulated procedure for microextraction of triazines (TZs) and sulfonamides (SAs). The solid supporting material was mixed with the MIL to overcome difficulties concerning the weighing of MIL and to control the uniform dispersion of the MIL, rendering the whole extraction procedure more reproducible...
August 7, 2018: Journal of Chromatography. A
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