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https://www.readbyqxmd.com/read/30554445/bringing-benign-ectomesenchymal-odontogenic-tumours-to-the-lab-an-in-vitro-study-using-an-organotypic-culture-model
#1
Victor Coutinho Bastos, Núbia Braga Pereira, Marina Gonçalves Diniz, Luciana Oliveira Andrade, Wagner Henriques Castro, Gregory Thomas Kitten, Ricardo Santiago Gomez, Carolina Cavalieri Gomes
BACKGROUND: Benign neoplasms exhibit most of the cellular phenomena considered hallmarks of cancer, except the capacity to metastasize. Thus, the elucidation of the mechanisms associated with the progression of benign neoplasms may complement and clarify the mechanisms involved in carcinogenesis. Benign odontogenic tumours often result in facial deformities and morbidities, and have complex pathogenesis, mainly due to the diversity of interactions between the odontogenic epithelium and the ectomesenchyme...
December 16, 2018: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/30554427/overcompensation-for-insect-herbivory-a-review-and-meta-analysis-of-the-evidence
#2
Loriann C Garcia, Micky D Eubanks
Not all herbivory is detrimental to plants. In some cases, plants can compensate for herbivory and maintain growth and fitness following damage or even overcompensate for herbivory and perform better than if left undamaged. Examples of overcompensation to vertebrate herbivory are well known, but here we review the literature for examples of reproductive overcompensation (i.e., increased production of traits associated with fitness) and increased vegetative growth (i.e., vegetative overcompensation) following insect herbivory...
December 16, 2018: Ecology
https://www.readbyqxmd.com/read/30554425/house-dust-mite-drives-pro-inflammatory-eicosanoid-reprogramming-and-macrophage-effector-functions
#3
Fiona D R Henkel, Antonie Friedl, Mark Haid, Dominique Thomas, Tiffany Bouchery, Pascal Haimerl, Marta de Los Reyes Jiménez, Francesca Alessandrini, Carsten B Schmidt-Weber, Nicola L Harris, Jerzy Adamski, Julia Esser-von Bieren
BACKGROUND: Eicosanoid lipid mediators play key roles in type 2 immune responses, e.g. in allergy and asthma. Macrophages represent major producers of eicosanoids and they are key effector cells of type 2 immunity. We aimed to comprehensively track eicosanoid profiles during type 2 immune responses to house dust mite (HDM) or helminth infection and to identify mechanisms and functions of eicosanoid reprogramming in human macrophages. METHODS: We established an LC-MS/MS workflow for the quantification of 52 oxylipins to analyze mediator profiles in human monocyte derived macrophages (MDM) stimulated with HDM and during allergic airway inflammation (AAI) or nematode infection in mice...
December 16, 2018: Allergy
https://www.readbyqxmd.com/read/30554395/overcoming-bioethical-legal-and-hereditary-barriers-to-mitochondrial-replacement-therapy-in-the-usa
#4
Marybeth Pompei, Francesco Pompei
The purpose of the paper is to explore novel means to overcome the controversial ban in the USA against mitochondrial replacement therapy, a form of IVF, with the added step of replacing a woman's diseased mutated mitochondria with a donor's healthy mitochondria to prevent debilitating and often fatal mitochondrial diseases. Long proven effective in non-human species, MRT recently performed in Mexico resulted in the birth of a healthy baby boy. We explore the ethics of the ban, the concerns over hereditability of mitochondrial disease and its mathematical basis, the overlooked role of Mitochondrial Eve, the financial burden of mitochondrial diseases for taxpayers, and a woman's reproductive rights...
December 15, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30554394/a-subspecialty-of-the-assisted-reproductive-technologies-cryogenic-inventory-maintenance
#5
EDITORIAL
Kathryn J Go
No abstract text is available yet for this article.
December 15, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30554323/illumina-miseq-sequencing-investigation-of-microbiota-in-bronchoalveolar-lavage-fluid-and-cecum-of-the-swine-infected-with-prrsv
#6
Nan Jiang, Huan Liu, Peng Wang, Jing Huang, Hui Han, Qinfu Wang
Porcine reproductive and respiratory syndrome virus (PRRSV) causes significant animal morbidity and mortality and economic losses worldwide. In this study, we analyzed the microbiota in bronchoalveolar lavage fluid (BAL), mucosa, and feces in cecum of the PRRSV-challenged pigs using the Illumina MiSeq sequencing platform, to investigate the role of microbiota in the pathogenesis and development of porcine reproductive and respiratory syndrome (PRRS). Quantitative insights into microbial ecology analyses indicated that the dominant bacterial groups in the lung from the PRRSV-challenged pigs were Haemophilus parasuis and Mycoplasma hyorhinis, with a relative abundance of 35-48% and 27-41%, respectively...
December 15, 2018: Current Microbiology
https://www.readbyqxmd.com/read/30554295/establishment-and-application-of-distant-hybridization-technology-in-fish
#7
REVIEW
Shi Wang, Chenchen Tang, Min Tao, Qinbo Qin, Chun Zhang, Kaikun Luo, Rurong Zhao, Jing Wang, Li Ren, Jun Xiao, Fangzhou Hu, Rong Zhou, Wei Duan, Shaojun Liu
Hybridization is widely used. However, for a long time, systematic theories and technologies related to hybridization in fish have been lacking. In this study, through long-term systematic research, we investigated and obtained the main rules regarding inheritance and reproduction related to fish distant hybridization. Furthermore, we established one-step and multistep breeding technologies that were suitable for interspecific hybridization and intraspecific hybridization. Simultaneously, we used these two breeding technologies to produce a batch of diploid fish lineages and tetraploid fish lineages and improved fishes...
December 13, 2018: Science China. Life Sciences
https://www.readbyqxmd.com/read/30554235/microbiological-surveillance-of-flexible-bronchoscopes-after-a-high-level-disinfection-with-peracetic-acid-preliminary-results-from-an-italian-teaching-hospital
#8
G Troiano, A Lo Nostro, C Calonico, N Nante, L Magistri, M B Pulci, F Niccolini
BACKGROUND: Flexible bronchoscopes are heat labile, complex and difficult to clean, and some nosocomial outbreaks related to bronchoscopy have been reported in literature. The aim of our study was to determine, through a systematic monitoring, whether bronchoscopes' cleaning and disinfection procedures have been correctly adopted by health operators. METHODS: We conducted a 19 months-long prospective study in the Unit of Pulmonology at Careggi Teaching Hospital (Florence, Italy), analyzing endoscopes that were reprocessed through a high-level disinfection procedure...
January 2019: Annali di Igiene: Medicina Preventiva e di Comunità
https://www.readbyqxmd.com/read/30554224/optimal-endobronchial-ultrasound-strain-elastography-assessment-strategy-an-explorative-study
#9
Roel L J Verhoeven, Chris L de Korte, Erik H F M van der Heijden
BACKGROUND: In lung cancer staging, mediastinal lymph nodes are currently aspirated using endobronchial ultrasound transbronchial needle aspiration (EBUS-TBNA) based on size and FDG-PET avidity. EBUS strain elastography (SE) is a new technique that may help predict the presence of malignancy. However, a standardized assessment strategy for EBUS-SE measurement is lacking. OBJECTIVES: The aim of this study was to determine the optimal assessment strategy for investigating the predictive value of EBUS-SE in mediastinal lymph nodes...
December 14, 2018: Respiration; International Review of Thoracic Diseases
https://www.readbyqxmd.com/read/30554099/cancer-diagnosis-using-nanomaterials-based-electrochemical-nanobiosensors
#10
REVIEW
Majid Sharifi, Mohammad Reza Avadi, Farnoosh Attar, Fariba Dashtestani, Hedayatollah Ghorchian, Seyed Mahdi Rezayat, Ali Akbar Saboury, Mojtaba Falahati
Cancer is one of the most important causes of mortality in the world, which can be severely reduced by early detection to avoid future problems in the field of economics and mental health. Hence, electrochemical nanobiosensors as portable devices for rapid detection of  cancer biomarkers, have found an important place in clinical medicine for diagnosis, managements or cancer screening. Although, these biosensors have been receiving attention in the recent years, their principles are unchanged. By progress in nanotechnology, a great potential has been giving to nanobiosensors...
November 19, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/30554050/bioaccumulation-and-morphological-traits-in-a-multi-generation-test-with-two-daphnia-species-exposed-to-lead
#11
G S Araujo, M D Pavlaki, A M V M Soares, D M S Abessa, S Loureiro
Anthropic pressure negatively affects natural environments. Lead (Pb) is a non-essential highly toxic metal that is present in aquatic ecosystems. Two daphnid species from two different latitudes, the temperate Daphnia magna and the tropical Daphnia similis were used as test-organisms to evaluate a long-term Pb exposure. Both species were exposed for nine generations to a chronic concentration of Pb (50 μg/L) and the effects were explored, considering some endpoints not commonly used in toxicity tests: body burden of Pb and presence of granules in the dorsal region of neonates, hemoglobin contents, carapace deformation and morphology, production of males and ephippia (or dormant haploid egg), changes in the eggs' colour and eggs abortion...
December 7, 2018: Chemosphere
https://www.readbyqxmd.com/read/30554036/auspicious-or-suspicious-does-religiosity-really-promote-elder-well-being-examining-the-belief-as-benefit-effect-among-older-japanese
#12
James Benjamin Schuurmans-Stekhoven
Recent findings suggest that the belief-as-benefit effect (BABE) - the positive association between religiosity and health/well-being - is a spurious correlation voided by personality traits. The current paper investigates the cross-sectional relationships among personality, religiosity and psychological well-being in an older adult sample randomly-selected from Tokyo, Japan. Correlation and Hierarchical Regression Modelling (HRM)-with a two one-sided test (TOST) of equivalence-is utilized. The standard BABE correlation is reproduced...
December 7, 2018: Archives of Gerontology and Geriatrics
https://www.readbyqxmd.com/read/30554028/mechanical-regulation-of-genome-architecture-and-cell-fate-decisions
#13
REVIEW
G V Shivashankar
Landmark experiments in vitro showed that somatic cells can be reprogrammed to stem-cells by the constitutive expression of particular transcription factors. However, in vivo cells naturally exhibit de-differentiation and trans-differentiation programs, thereby suggesting that the signals from the local mechanical microenvironment may be sufficient to induce stem-cell state transitions. In this review, we discuss recent evidence for the biophysical regulation of genome architecture and nuclear programs. We start by discussing the coupling between cellular architecture, genome organization and gene expression...
December 13, 2018: Current Opinion in Cell Biology
https://www.readbyqxmd.com/read/30554025/chemical-induced-conversion-of-mouse-fibroblasts-and-human-adipose-derived-stem-cells-into-skeletal-muscle-like-cells
#14
Varun Bansal, Debojyoti De, Jieun An, Tong Mook Kang, Hyeon-Ju Jeong, Jong-Sun Kang, Kyeong Kyu Kim
Use of stem cells in regenerative medicine holds great promise in treating people suffering from various otherwise incurable ailments. Direct conversion of somatic cells to other lineages thereby bypassing the intermediate pluripotent state has enormous applicability with respect to time requirement for conversion as well as safety issues. Among various approaches, chemical induced cell conversion is safe yet effective, and the use of small molecules has thus increased greatly in recent years in regenerative fields due to easy applicability, efficient scalability, and consistent reproducibility...
December 6, 2018: Biomaterials
https://www.readbyqxmd.com/read/30553979/testicular-morphology-and-spermatogenesis-in-harbour-porpoises-phocoena-phocoena
#15
T Kesselring, S Viquerat, L L IJsseldijk, M Langeheine, P Wohlsein, A Gröne, M Bergmann, U Siebert, R Brehm
Knowledge about reproductive parameters in male harbour porpoises such as testicular histology and germ cell maturation as well as seasonal changes in spermatogenesis is scarce. Thus, the aim of the present study was to report changes in the histological appearance of the testicular morphology of neonatal and juvenile harbour porpoises during maturation, to identify stages of spermatogenesis in adult males and to detect seasonal modifications. The identification of these stages can be used to assess the developmental profile of gene expression during spermatogenesis and to identify defects in spermatogenesis arising in pathological conditions...
November 28, 2018: Theriogenology
https://www.readbyqxmd.com/read/30553978/induction-of-spermiation-using-ovaprim%C3%A2-with-topical-gill-method-in-the-silver-rasbora-rasbora-argyrotaenia
#16
Lutfiyah Al Adawiyah, Laksmi Sulmartiwi, Türker Bodur, Darmawan Setia Budi
The main obstacles on silver rasbora (Rasbora argyrotaenia) culture are having the limited number of broodstock and spawning depending on the season. The purpose of this study was to determine the effect of different dosage of Ovaprim™ induction by topical gill method to silver rasbora spermiation in order to continue the production out of its reproduction season with an optimum dose. A total of 30 male fish with a weight of 7.78 ± 0.20 g and length 4.11 ± 0.31 cm was used in this research. Topical gill treatments of Ovaprim™ were administered with following doses; 0...
December 8, 2018: Theriogenology
https://www.readbyqxmd.com/read/30553977/mirna-185-regulates-retained-fetal-membranes-of-cattle-by-targeting-stim1
#17
REVIEW
C Y Zheng, X Zou, B C Zhao, M L Zhang, H J Lin, C H Luo, Z M Xu, L Y Shao, S X Fu
Retained fetal membranes (RFM) of cows is an important reproductive disturbance, and is related to miRNA-185. Stromal interaction molecule 1 (STIM1), a potential target gene of miRNA-185, could influence placenta release via regulating Ca2+ concentration intracellular. The aim of this study was to explore the mechanism of RFM by investigating the regulatory relationship between miRNA-185 and STIM1 in primary uterine caruncel epithelial (UCE) cells. Serum samples of healthy Holstein dairy cows (n = 20) and RFM cows (n = 12), with a similar age, parity, weight, and milk yield, were collected to detect Ca2+ concentration at prepartum 1-5 d and postpartum 6, 12 and 24 h...
November 27, 2018: Theriogenology
https://www.readbyqxmd.com/read/30553967/the-pdz-gef-gef26-regulates-synapse-development-and-function-via-fasii-and-rap1-at-the-drosophila-neuromuscular-junction
#18
Mengzhu Ou, Su Wang, Mingkuan Sun, Jinsong An, Huihui Lv, Xiankun Zeng, Steven X Hou, Wei Xie
Guanine nucleotide exchange factors (GEFs) are essential for small G proteins to activate their downstream signaling pathways, which are involved in morphogenesis, cell adhesion, and migration. Mutants of Gef26, a PDZ-GEF (PDZ domain-containing guanine nucleotide exchange factor) in Drosophila, exhibit strong defects in wings, eyes, and the reproductive and nervous systems. However, the precise roles of Gef26 in development remain unclear. In the present study, we analyzed the role of Gef26 in synaptic development and function...
December 13, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/30553966/a-novel-in-vitro-co-culture-model-to-examine-contact-formation-between-astrocytic-processes-and-cerebral-vessels
#19
Shoji Kawauchi, Sayo Horibe, Naoto Sasaki, Ken-Ichi Hirata, Yoshiyuki Rikitake
Here, we developed a novel in vitro co-culture model, in which process-bearing astrocytes and isolated cerebral microvessels from mice were co-cultured. Astrocytes formed contacts with microvessels from both adult and neonatal mice. However, concentrated localization of the immunofluorescence signal for aquaporin-4 (AQP4) at contact sites between perivascular endfoot processes and blood vessels was only detected with neonatal mouse microvessels. Contact between astrocytic processes and microvessels was retained, whereas concentrated localization of AQP4 signal at contact sites was lost, by knockdown of dystroglycan or α-syntrophin, reflecting polarized localization of AQP4 at perivascular regions in the brain...
December 13, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/30553945/reproducibility-of-diaphragmatic-thickness-measured-by-m-mode-ultrasonography-in-healthy-volunteers
#20
Scarlata Simone, Mancini Damiana, Laudisio Alice, Antonelli Incalzi Raffaele
We aimed at testing M-mode ultrasound diaphragmatic thickness reproducibility and its clinical correlates in healthy volunteers. Sixty-six consecutive healthy volunteers were considered eligible, and enrolled in the study. During a single visit, all participants received three M-mode and B-mode ultrasound evaluations of Diaphragmatic Thickness (DT). We then proceeded to calculate the thickness difference and assess the association of ultrasonographic measurements with demographic and anthropometric data. Variables associated through univariate analyses were entered in multivariable models, and Intraclass Correlation Coefficient (ICC) was performed in order to determine intra- and inter-observer reproducibility...
December 13, 2018: Respiratory Physiology & Neurobiology
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