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https://www.readbyqxmd.com/read/30317841/changes-in-muscle-hardness-after-a-full-marathon-appear-different-even-intramuscularly
#1
Takayuki Inami, Kento Nakagawa, Mako Fukano, Takahisa Yonezu, Ayako Higashihara, Satoshi Iizuka, Takaya Narita, Mitsuyoshi Murayama
No abstract text is available yet for this article.
October 10, 2018: Journal of Sports Medicine and Physical Fitness
https://www.readbyqxmd.com/read/30283390/perceptual-restoration-of-temporally-distorted-speech-in-l1-vs-l2-local-time-reversal-and-modulation-filtering
#2
Mako Ishida, Takayuki Arai, Makio Kashino
Speech is intelligible even when the temporal envelope of speech is distorted. The current study investigates how native and non-native speakers perceptually restore temporally distorted speech. Participants were native English speakers (NS), and native Japanese speakers who spoke English as a second language (NNS). In Experiment 1, participants listened to "locally time-reversed speech" where every x-ms of speech signal was reversed on the temporal axis. Here, the local time reversal shifted the constituents of the speech signal forward or backward from the original position, and the amplitude envelope of speech was altered as a function of reversed segment length...
2018: Frontiers in Psychology
https://www.readbyqxmd.com/read/30255610/red-shifted-fluorogenic-substrate-for-detecting-lacz-positive-cells-in-living-tissue-with-single-cell-resolution
#3
Hiroki Ito, Yu Kawamata, Mako Kamiya, Kayoko Tsuda-Sakurai, Shinji Tanaka, Tasuku Ueno, Toru Komatsu, Kenjiro Hanaoka, Shigeo Okabe, Masayuki Miura, Yasuteru Urano
The Escherichia coli lacZ gene encoding β-galactosidase is a widely used reporter, but few synthetic substrates are available for detecting its activity with single-cell resolution in living samples. Our recently reported fluorogenic substrate SPiDER-βGal is suitable for this purpose, but its hydrolysis product shows green fluorescence emission, and a red-shifted analogue is required for use in combination with green fluorescent protein (GFP) markers. Here, we describe the development of a red-shifted fluorogenic substrate for β-galactosidase, SPiDER-Red-βGal, based on silicon rhodol scaffold and by using a carboxylic group as the intramolecular nucleophile...
September 25, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/30188508/sarcomere-gene-variants-act-as-a-genetic-trigger-underlying-the-development-of-left-ventricular-noncompaction
#4
Asami Takasaki, Keiichi Hirono, Yukiko Hata, Ce Wang, Masafumi Takeda, Jun K Yamashita, Bo Chang, Hideyuki Nakaoka, Mako Okabe, Nariaki Miyao, Kazuyoshi Saito, Keijiro Ibuki, Sayaka Ozawa, Michikazu Sekine, Naoki Yoshimura, Naoki Nishida, Neil E Bowles, Fukiko Ichida
BACKGROUND: Left ventricular noncompaction (LVNC) is a primary cardiomyopathy with heterogeneous genetic origins. The aim of this study was to elucidate the role of sarcomere gene variants in the pathogenesis and prognosis of LVNC. METHODS AND RESULTS: We screened 82 Japanese patients (0-35 years old), with a diagnosis of LVNC, for mutations in seven genes encoding sarcomere proteins, by direct DNA sequencing. We identified variants in a significant proportion of cases (27%), which were associated with poor prognosis (p = 0...
September 6, 2018: Pediatric Research
https://www.readbyqxmd.com/read/30185909/in-vivo-reprogramming-of-wound-resident-cells-generates-skin-epithelial-tissue
#5
Masakazu Kurita, Toshikazu Araoka, Tomoaki Hishida, David D O'Keefe, Yuta Takahashi, Akihisa Sakamoto, Masahiro Sakurai, Keiichiro Suzuki, Jun Wu, Mako Yamamoto, Reyna Hernandez-Benitez, Alejandro Ocampo, Pradeep Reddy, Maxim Nikolaievich Shokhirev, Pierre Magistretti, Estrella Núñez Delicado, Hitomi Eto, Kiyonori Harii, Juan Carlos Izpisua Belmonte
Large cutaneous ulcers are, in severe cases, life threatening1,2 . As the global population ages, non-healing ulcers are becoming increasingly common1,2 . Treatment currently requires the transplantation of pre-existing epithelial components, such as skin grafts, or therapy using cultured cells2 . Here we develop alternative supplies of epidermal coverage for the treatment of these kinds of wounds. We generated expandable epithelial tissues using in vivo reprogramming of wound-resident mesenchymal cells. Transduction of four transcription factors that specify the skin-cell lineage enabled efficient and rapid de novo epithelialization from the surface of cutaneous ulcers in mice...
September 2018: Nature
https://www.readbyqxmd.com/read/30140132/patient-reported-and-clinical-outcomes-of-robotic-arm-assisted-unicondylar-knee-arthroplasty-minimum-two-year-follow-up
#6
J Melvin Deese, Gwen Gratto-Cox, Denny A Carter, Thomas M Sasser, Karah L Brown
Background: Unicompartmental knee arthroplasty (UKA) originated in the 1950's. There have been many enhancements to the implants and the technique, improving the precision and accuracy of this challenging operation. Specifically for Robotic Arm Interactive Orthopedic System (Rio; Mako Stryker, Fort Lauderdale, FL), there are many studies reporting clinical outcomes, but our search offered nothing regarding patient reported outcomes using validated surveys. Methods: Patients with onlay tibial components presenting for routine follow-up of robotic-arm assisted UKA performed between May 2009 and September 2013 were invited to participate...
September 2018: Journal of Orthopaedics
https://www.readbyqxmd.com/read/30125435/reactive-peripheral-blood-plasmacytosis-in-kawasaki-disease
#7
Mako Okabe, Keiichi Hirono, Kentaro Tamura, Fukiko Ichida, Hirokazu Kanegane
No abstract text is available yet for this article.
September 2018: Pediatrics International: Official Journal of the Japan Pediatric Society
https://www.readbyqxmd.com/read/30122738/clinical-and-echocardiographic-impact-of-tafazzin-variants-on-dilated-cardiomyopathy-phenotype-in-left-ventricular-non-compaction-patients-in-early-infancy
#8
Keiichi Hirono, Yukiko Hata, Makoto Nakazawa, Nobuo Momoi, Tohru Tsuji, Taro Matsuoka, Mamoru Ayusawa, Yuriko Abe, Tamaki Hayashi, Nobuyuki Tsujii, Tadaaki Abe, Heima Sakaguchi, Ce Wang, Asami Takasaki, Shinya Takarada, Mako Okabe, Nariaki Miyao, Hideyuki Nakaoka, Keijiro Ibuki, Kazuyoshi Saito, Sayaka Ozawa, Naoki Nishida, Neil E Bowles, Fukiko Ichida
BACKGROUND: Left ventricular non-compaction (LVNC) is a cardiomyopathy morphologically characterized by 2-layered myocardium and numerous prominent trabeculations, and is often associated with dilated cardiomyopathy (DCM). Variants in the gene encoding tafazzin (TAZ) may change mitochondrial function and cause dysfunction of many organs, but they also contribute to the DCM phenotype in LVNC, and the clinical and echocardiographic features of children with this phenotype are poorly understood...
September 25, 2018: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/30086450/sex-differences-in-three-dimensional-talocrural-and-subtalar-joint-kinematics-during-stance-phase-in-healthy-young-adults
#9
Mako Fukano, Toru Fukubayashi, Scott Arthur Banks
The ankle joint, including the talocrural and subtalar joints, plays an important role in human locomotion. Sex differences in walking patterns among young and old adults have been studied; however, little information exists on sex-based variations in talocrural and subtalar joint kinematics during walking. Thus, the purpose of this study was to investigate sex-based differences in the talocrural and subtalar joint kinematics during walking. We obtained lateral fluoroscopic images from 10 male and 7 female healthy volunteers during stance phase, and determined the three-dimensional bone orientations using 3D-2D model-image registration techniques to compare sex-specific differences...
October 2018: Human Movement Science
https://www.readbyqxmd.com/read/30082826/hybrid-cell-reactor-system-from-escherichia-coli-protoplast-cells-and-arrayed-lipid-bilayer-chamber-device
#10
Yoshiki Moriizumi, Kazuhito V Tabata, Rikiya Watanabe, Tomohiro Doura, Mako Kamiya, Yasuteru Urano, Hiroyuki Noji
We developed a novel hybrid cell reactor system via functional fusion of single Escherichia coli protoplast cells, that are deficient in cell wall and expose plasma membrane, with arrayed lipid bilayer chambers on a device in order to incorporate the full set of cytosolic and membrane constituents into the artificial chambers. We investigated gene expression activity to represent the viability of the hybrid cell reactors: over 20% of hybrid cells showed gene expression activity from plasmid or mRNA. This suggests that the hybrid cell reactors retained fundamental activity of genetic information transduction...
August 6, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30082124/hydrophobic-deep-eutectic-solvents-as-green-extraction-media-for-polycyclic-aromatic-hydrocarbons-in-aqueous-samples
#11
Patrycja Makoś, Andrzej Przyjazny, Grzegorz Boczkaj
The paper presents novel nonionic and hydrophobic deep eutectic solvents which were synthesized from natural compounds, i.e., thymol, ±camphor, decanoic and 10-undecylenic acids. Fundamental physicochemical properties of the synthesized deep eutectic solvents were determined, followed by their application as extractants in ultrasound-assisted dispersive liquid-liquid microextraction to isolate and enrich polycyclic aromatic hydrocarbons from aqueous samples characterized by a complex matrix. The final determination was carried out by gas chromatography-mass spectrometry...
October 5, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/30072799/postpartum-depression-among-women-in-nagoya-indirectly-exposed-to-the-great-east-japan-earthquake
#12
Chika Kubota, Takashi Okada, Mako Morikawa, Yukako Nakamura, Aya Yamauchi, Masahiko Ando, Tomoko Shiino, Masako Ohara, Satomi Murase, Setsuko Goto, Atsuko Kanai, Tomoko Masuda, Branko Aleksic, Norio Ozaki
This study aimed to assess the situation of postpartum depression and maternal bonding in Nagoya, a city distant from the epicenter of the Great East Japan Earthquake that occurred on March 11, 2011. Among the participants at 1 month after childbirth between March 11, 2010 and March 10, 2013 (n = 188), 152 fully responded to the Edinburgh Postnatal Depression Scale (EPDS) and Mother-Infant Bonding Questionnaire (MIBQ). They were divided into pre-quake (n = 58), and 0-6, 6-12, 12-18, and 18-24 months after the earthquake groups (n = 20, 26, 29, and 19, respectively)...
August 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30035682/foot-posture-alteration-and-recovery-following-a-full-marathon-run
#13
Mako Fukano, Takayuki Inami, Kento Nakagawa, Takaya Narita, Shigeo Iso
Prolonged running results in lowering of the foot arch and a low arch is associated with subsequent chronic injuries. Foot posture alteration and recovery following a marathon run remain unknown. Therefore, the present study aimed to evaluate foot posture alteration following a full marathon run. The three-dimensional foot posture data of 11 collegiate runners were obtained using an optical foot scanner system before, and immediately, 1 day, 3 days, and 8 days after a full marathon. The navicular height and arch height ratio significantly decreased from before to immediately, 1 day, 3 days, and 8 days after the marathon (navicular height: before, 44...
November 2018: European Journal of Sport Science
https://www.readbyqxmd.com/read/30033352/o-017-a-five-year-follow-up-of-gait-in-robotic-assisted-vs-conventional-unicompartmental-knee-arthroplasty
#14
Lindsay Jane Millar, Matthew Banger, Philip John Rowe, Mark Blyth, Bryn Jones, Angus Maclean
Recently, systems have been developed to improve alignment of unicompartmental knee arthroplasty (UKA) implants, although improvement in function has been difficult to document. The MAKO RIO robotic surgery system has previously shown improvements in in knee flexion during weight acceptance (WA) in comparison to conventional methods at a one year follow up. This study aimed to determine if these improvements remained at five years follow up. Twenty five MAKO and 21 conventional knees were tested using three dimensional gait analysis to measure knee kinematics...
September 2018: Gait & Posture
https://www.readbyqxmd.com/read/30030372/stress-granule-formation-is-induced-by-a-threshold-temperature-rather-than-a-temperature-difference-in-arabidopsis
#15
Takahiro Hamada, Mako Yako, Marina Minegishi, Mayuko Sato, Yasuhiro Kamei, Yuki Yanagawa, Kiminori Toyooka, Yuichiro Watanabe, Ikuko Hara-Nishimura
Stress granules, a type of cytoplasmic RNA granule in eukaryotic cells, are induced in response to various environmental stresses, including high temperature. However, how high temperatures induce the formation of these stress granules in plant cells is largely unknown. Here, we characterized the process of stress granule formation in Arabidopsis thaliana by combining live imaging and electron microscopy analysis. In seedlings grown at 22°C, stress granule formation was induced at temperatures above a critical threshold level of 34°C in the absence of transpiration...
August 20, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/30021529/association-between-tongue-pressure-and-subclinical-carotid-atherosclerosis-in-relation-to-platelet-levels-in-hypertensive-elderly-men-a-cross-sectional-study
#16
Yuji Shimizu, Shimpei Sato, Yuko Noguchi, Jun Koyamatsu, Hirotomo Yamanashi, Miho Higashi, Mako Nagayoshi, Shin-Ya Kawashiri, Yasuhiro Nagata, Noboru Takamura, Takahiro Maeda
BACKGROUND: Age-related low-grade inflammation causing endothelial disruption influences sarcopenia, hypertension, and atherosclerosis. We reported previously that maintenance of muscle strength in elderly hypertensive men with high platelet levels is positively associated with subclinical atherosclerosis but not in those with low platelet levels. Since reduced tongue pressure is related to sarcopenia, tongue pressure may be associated with subclinical carotid atherosclerosis in hypertensive elderly subjects, and platelet levels may function as an indicator of the association between tongue pressure and subclinical carotid atherosclerosis...
July 18, 2018: Environmental Health and Preventive Medicine
https://www.readbyqxmd.com/read/30019537/effects-of-maternity-yoga-on-the-autonomic-nervous-system-during-pregnancy
#17
Mako Hayase, Mieko Shimada
AIM: To elucidate the changes in the daily variation seen in the autonomic nervous system during pregnancy brought about by maternity yoga and to evaluate how maternal yoga affects stress and sleep. METHODS: A prospective, longitudinal study was conducted between January 2013 and December 2014. Pregnant women who were attending maternity yoga classes at Palmore Hospital between 20 and 23 weeks' gestation were enrolled as the yoga group, and a matched control group was also enrolled...
October 2018: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/30002632/validation-and-factor-analysis-of-the-japanese-version-of-the-highs-scale-in-perinatal-women
#18
Aya Yamauchi, Takashi Okada, Masahiko Ando, Mako Morikawa, Yukako Nakamura, Chika Kubota, Masako Ohara, Satomi Murase, Setsuko Goto, Atsuko Kanai, Norio Ozaki
Background: The Highs scale has been developed to evaluate hypomanic symptoms in the first postpartum week. However, it has not been elucidated whether this scale is also applicable to pregnant women. To address this issue, we confirmed the factor structure, reliability, and validity of the Japanese version of the Highs scale for pregnant and postpartum women. Methods: 418 women provided effective responses to both the Highs scale and the Edinburgh Postnatal Depression Scale (EPDS) during early pregnancy (before week 25), late pregnancy (around week 36), at 5 days and at 1 month after delivery...
2018: Frontiers in Psychiatry
https://www.readbyqxmd.com/read/30001384/aif1l-regulates-actomyosin-contractility-and-filopodial-extensions-in-human-podocytes
#19
Mako Yasuda-Yamahara, Manuel Rogg, Kosuke Yamahara, Jasmin I Maier, Tobias B Huber, Christoph Schell
Podocytes are highly-specialized epithelial cells essentially required for the generation and the maintenance of the kidney filtration barrier. This elementary function is directly based on an elaborated cytoskeletal apparatus establishing a complex network of primary and secondary processes. Here, we identify the actin-bundling protein allograft-inflammatory-inhibitor 1 like (AIF1L) as a selectively expressed podocyte protein in vivo. We describe the distinct subcellular localization of AIF1L to actin stress fibers, focal adhesion complexes and the nuclear compartment of podocytes in vitro...
2018: PloS One
https://www.readbyqxmd.com/read/29984063/pancreatic-compression-during-lymph-node-dissection-in-laparoscopic-gastrectomy-possible-cause-of-pancreatic-leakage
#20
Satoshi Ida, Naoki Hiki, Takeaki Ishizawa, Yugo Kuriki, Mako Kamiya, Yasuteru Urano, Takuro Nakamura, Yasuo Tsuda, Yosuke Kano, Koshi Kumagai, Souya Nunobe, Manabu Ohashi, Takeshi Sano
Purpose: Postoperative pancreatic fistula is a serious and fatal complication of gastrectomy for gastric cancer. Blunt trauma to the parenchyma of the pancreas can result from an assistant's forceps compressing and retracting the pancreas, which in turn may result in pancreatic juice leakage. However, no published studies have focused on blunt trauma to the pancreas during laparoscopic surgery. Our aim was to investigate the relationship between compression of the pancreas and pancreatic juice leakage in a swine model...
June 2018: Journal of Gastric Cancer
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