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Hidemasa Kubo, Kenjiro Hanaoka, Yugo Kuriki, Toru Komatsu, Tasuku Ueno, Ryosuke Kojima, Mako Kamiya, Yasutoshi Murayama, Eigo Otsuji, Yasuteru Urano
Rapid diagnosis of metastatic lymph nodes (mLNs) of colorectal cancer (CRC) is desirable either intraoperatively or in resected fresh specimens. We have developed a series of activatable fluorescence probes for peptidase activities that are specifically upregulated in various tumors. We aimed to discover a target enzyme for detecting mLNs of CRC. Among our probes, we found that gGlu-HMRG, a gamma-glutamyl transpeptidase (GGT)-activatable fluorescence probe, could detect mLNs. This was unexpected, because we have previously reported that gGlu-HMRG could not detect primary CRC...
December 12, 2018: Scientific Reports
Shohei Kojima, Ryo Sato, Mako Yanai, Yumiko Komatsu, Masayuki Horie, Manabu Igarashi, Keizo Tomonaga
Targeting of viral proteins to specific subcellular compartments is a fundamental step for viruses to achieve successful replication in infected cells. Borna disease virus-1 (BoDV), a non-segmented, negative-strand RNA virus, uniquely replicates and persists in the cell nucleus. Here, it is demonstrated that BoDV nucleoprotein (N) transcripts undergo mRNA splicing to generate truncated isoforms. In combination with alternative usage of translation initiation sites, the N gene potentially expresses at least six different isoforms, which exhibit diverse intracellular localizations, including the nucleoplasm, cytoplasm and endoplasmic reticulum (ER), as well as intranuclear viral replication sites...
December 12, 2018: Journal of Virology
Teresa L Mako, Joan M Racicot, Mindy Levine
There is great need for stand-alone luminescence-based chemosensors that exemplify selectivity, sensitivity, and applicability and that overcome the challenges that arise from complex, real-world media. Discussed herein are recent developments toward these goals in the field of supramolecular luminescent chemosensors, including macrocycles, polymers, and nanomaterials. Specific focus is placed on the development of new macrocycle hosts since 2010, coupled with considerations of the underlying principles of supramolecular chemistry as well as analytes of interest and common luminophores...
December 3, 2018: Chemical Reviews
Christoph Schell, Benedikt Sabass, Martin Helmstaedter, Felix Geist, Ahmed Abed, Mako Yasuda-Yamahara, August Sigle, Jasmin I Maier, Florian Grahammer, Florian Siegerist, Nadine Artelt, Nicole Endlich, Dontscho Kerjaschki, Hans-Henning Arnold, Jörn Dengjel, Manuel Rogg, Tobias B Huber
Podocytes, highly specialized epithelial cells, build the outer part of the kidney filtration barrier and withstand high mechanical forces through a complex network of cellular protrusions. Here, we show that Arp2/3-dependent actin polymerization controls actomyosin contractility and focal adhesion maturation of podocyte protrusions and thereby regulates formation, maintenance, and capacity to adapt to mechanical requirements of the filtration barrier. We find that N-WASP-Arp2/3 define the development of complex arborized podocyte protrusions in vitro and in vivo...
November 26, 2018: Developmental Cell
Rumi Hino, Naoko Inoshita, Toyoki Yoshimoto, Makiko Ogawa, Daishu Miura, Ryoko Watanabe, Kenta Watanabe, Mako Kamiya, Yasteru Urano
Background: Nodular lesions of the thyroid gland, including papillary thyroid carcinoma (PTC), may be difficult to diagnose by imaging, such as in ultrasonic echo testing, or by needle biopsy. Definitive diagnosis is made by pathological examination but takes several days. A more rapid and simple method to clarify whether thyroid nodular lesions are benign or malignant is needed. Fluorescence imaging with γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) uses γ-glutamyltranspeptidase (GGT), a cell-surface enzyme, to hydrolyze the γ-glutamyl peptide and transfer the γ-glutamyl group...
2018: Thyroid Research
Giulia Armaroli, Emely Verweyen, Carolin Pretzer, Katharina Kessel, Keiichi Hirono, Fukiko Ichida, Mako Okabe, David A Cabral, Dirk Foell, Kelly L Brown, Christoph Kessel
OBJECTIVE: Kawasaki disease (KD) is an acute vasculitis of childhood, predominantly affecting coronary arteries. S100A12 is a granulocyte-derived agonist of both the receptor for advanced glycation end products (RAGE) and toll-like receptor (TLR) 4. S100A12 is strongly upregulated in KD but it is unknown if or how it contributes to KD pathogenesis. METHODS: Serum samples from patients with KD at different stages pre- and post-intravenous immunoglobulin (IVIG) treatment were analyzed for S100A12, cytokines, chemokines and soluble markers of endothelial activation...
November 17, 2018: Arthritis & Rheumatology
Alan Marín, José Serna, Christian Robles, Beder Ramírez, Lorenzo E Reyes-Flores, Eliana Zelada-Mázmela, Giovanna Sotil, Ruben Alfaro
Peru is one of the world's leading fishing nations and its seafood industry relies on the trade of a vast variety of aquatic resources, playing a key role in the country's socio-economic development. DNA barcoding has become of paramount importance for systematics, conservation, and seafood traceability, complementing or even surpassing conventional identification methods when target organisms show similar morphology during the early life stages, have recently diverged, or have undergone processing. Aiming to increase our knowledge of the species diversity available across the Peruvian supply chain (from fish landing sites to markets and restaurants), we applied full and mini-barcoding approaches targeting three mitochondrial genes (COI, 16S, and 12S) and the control region to identify samples purchased at retailers from six departments along the north-central Peruvian coast...
2018: PloS One
John A Mohan, Nathan R Miller, Sharon Z Herzka, Oscar Sosa-Nishizaki, Suzanne Kohin, Heidi Dewar, Michael Kinney, Owyn Snodgrass, R J David Wells
As upper-level predators, sharks are important for maintaining marine food web structure, but populations are threatened by fishery exploitation. Sustainable management of shark populations requires improved understanding of migration patterns and population demographics, which has traditionally been sought through physical and/or electronic tagging studies. The application of natural tags such as elemental variations in mineralized band pairs of elasmobranch vertebrae cartilage could also reveal endogenous and exogenous processes experienced by sharks throughout their life histories...
November 7, 2018: Proceedings. Biological Sciences
Mako Wakabayashi, Seiji Futagami, Hiroshi Yamawaki, Atsushi Tatsuguchi, Keiko Kaneko, Shuhei Agawa, Kazutoshi Higuchi, Noriko Sakasegawa, Makoto Murakami, Teppei Akimoto, Yasuhiro Kodaka, Nobue Ueki, Kaya Gudis, Chiaki Kawamoto, Takashi Akamizu, Choitsu Sakamoto, Katsuhiko Iwakiri
BACKGROUND: There was no available data concerning the clinical differentiation between the updated definition of early chronic pancreatitis (ECP) and anti-acid therapy-resistant functional dyspepsia (RFD). AIMS: We aimed to determine whether clinical symptoms, gastric motility, psychogenic factors and fat intake can help distinguish early chronic pancreatitis (ECP) from anti-acid therapy-resistant functional dyspepsia patients with pancreatic enzyme abnormalities (RFD-P) and anti-acid therapy-resistant functional dyspepsia (RFD) patients using endosonography...
2018: PloS One
Yuji Shimizu, Hirotomo Yamanashi, Yuko Noguchi, Jun Koyamatsu, Mako Nagayoshi, Kairi Kiyoura, Shoichi Fukui, Mami Tamai, Shin-Ya Kawashiri, Kazuhiko Arima, Takahiro Maeda
BACKGROUND: Hemoglobin concentration reportedly is positively associated with muscle strength, for example, handgrip strength. However, hemoglobin cannot repair muscle directly, but is beneficial only in a supportive role. Since hepatocyte growth factor (HGF) regulates muscle satellite cell production and differentiation, which is stimulated by organ injury, the supportive effect of hemoglobin should thus be stronger for participants with high HGF than for those with low HGF. However, the association between hemoglobin concentration and handgrip strength in relation to HGF levels remains unknown...
November 6, 2018: Environmental Health and Preventive Medicine
Hiroshi Yamawaki, Seiji Futagami, Keiko Kaneko, Shuhei Agawa, Kazutoshi Higuchi, Makoto Murakami, Mako Wakabayashi, Noriko Sakasegawa, Yasuhiro Kodaka, Nobue Ueki, Katya Gudis, Chiaki Kawamoto, Katsuhiko Iwakiri
BACKGROUND/AIMS: The aims of the study are to clarify the pathophysiological differences among early chronic pancreatitis (ECP), functional dyspepsia with pancreatic (FD-P) enzyme abnormalities and FD patients and to determine whether camostat mesilate, pancrelipase, and rabeprazole triple therapy improve FD symptoms in the ECP patients and FD-P patients in cross-over way. METHODS: We enrolled 84 consecutive patients presenting with typical symptoms of FD patients (n = 42), ECP patients (n = 15), and FD-P patients (n = 27)...
November 2, 2018: Digestion
Shinshiro Mineta, Takayuki Inami, Mako Fukano, Takuma Hoshiba, Yuta Masuda, Akane Yoshimura, Tsukasa Kumai, Norikazu Hirose
OBJECTIVE: To assess the discriminability as well as the reliability, and internal consistency of the Identification of Functional Ankle Instability questionnaire translated into Japanese (IdFAI-J). DESIGN: Cross-sectional study. SETTING: Collegiate athletic training/sports medicine clinic. PARTICIPANTS: Twenty bilingual and sixty-five collegiate athletes including participants with and without functional instability (FI)...
October 24, 2018: Physical Therapy in Sport
Gareth L Jordaan, Jorge Santos, Johan C Groeneveld
The assumption of a proportional relationship between catch-per-unit-effort (CPUE) and the abundance of sharks caught by pelagic longliners is tenuous when based on fisher logbooks that report only retained specimens. Nevertheless, commercial logbooks and landings statistics are often the only data available for stock status assessments. Logbook data collected from local and foreign pelagic longline vessels operating in four areas off southern Africa between 2000 and 2015 were used to construct standardized CPUE indices for blue sharks Prionace glauca and shortfin makos Isurus oxyrinchus ...
2018: PeerJ
Jianghui Qin, Zhihong Xu, Jin Dai, Dongyang Chen, Xingquan Xu, Kai Song, Dongquan Shi, Qing Jiang
Robotic-arm facilitated orthopaedic surgeons perform individualized and precise arthroplasty surgery. This system (MAKO) has been recently introduced into China and conducted total hip arthroplasty in several central hospitals. The pre-operative preparation and surgical procedures, together with some precautions for surgery, are outlined here to familiarize this technique to the orthopaedic surgeons in China.
September 2018: Annals of Translational Medicine
Yuji Matsuyoshi, Mariko Akahoshi, Mako Nakamura, Ryuichi Tatsumi, Wataru Mizunoya
Satellite cells (SCs) are myogenic stem cells that play an important role in skeletal muscle regeneration and hypertrophy. Primary cultures of SCs are useful to analyze cell functions; however, it is difficult to obtain highly pure SCs from young rats with the conventional procedures. The purpose of this study is to establish a purification method for SC isolation from young rats and quantitatively evaluate the purification procedure employing Percoll, a common research tool to purify cells. We elucidated the purity of SCs collected by Percoll density gradient centrifugation using real-time RT-qPCR and immunocytochemistry for desmin...
2019: Methods in Molecular Biology
Seiji Futagami, Hiroshi Yamawaki, Shuhei Agawa, Kazutoshi Higuchi, Go Ikeda, Hiroto Noda, Kumiko Kirita, Teppei Akimoto, Mako Wakabayashi, Noriko Sakasegawa, Yasuhiro Kodaka, Nobue Ueki, Tetsuro Kawagoe, Katsuhiko Iwakiri
No abstract text is available yet for this article.
2018: Translational Gastroenterology and Hepatology
Kevin T Du Clos, Amy Lang, Sean Devey, Philip J Motta, Maria Laura Habegger, Brad J Gemmell
Shark skin has been shown to reduce drag in turbulent boundary layer flows, but the flow control mechanisms by which it does so are not well understood. Drag reduction has generally been attributed to static effects of scale surface morphology, but possible drag reduction effects of passive or active scale actuation, or 'bristling', have been recognized more recently. Here, we provide the first direct documentation of passive scale bristling due to reversing, turbulent boundary layer flows. We recorded and analysed high-speed videos of flow over the skin of a shortfin mako shark, Isurus oxyrinchus These videos revealed rapid scale bristling events with mean durations of approximately 2 ms...
October 24, 2018: Journal of the Royal Society, Interface
Krisztina Kopcsó, Ágnes Bornemisza, Hajnalka Sz Makó
INTRODUCTION: Although pregnancy related anxiety has significant, negative effects on the outcomes of mothers, birth and children, there is a lack of research in Hungary regarding its prevalence and characteristics. AIM: The aim of the present study was the adaptation of the Pregnancy Related Thoughts by Rini et al. for a Hungarian sample, including the investigation of its psychometric properties. METHOD: 248 pregnant women, aged 30.46 years (SD = 4...
October 2018: Orvosi Hetilap
Takayuki Inami, Kento Nakagawa, Mako Fukano, Takahisa Yonezu, Ayako Higashihara, Satoshi Iizuka, Takaya Narita, Mitsuyoshi Murayama
No abstract text is available yet for this article.
October 10, 2018: Journal of Sports Medicine and Physical Fitness
Mako Ishida, Takayuki Arai, Makio Kashino
Speech is intelligible even when the temporal envelope of speech is distorted. The current study investigates how native and non-native speakers perceptually restore temporally distorted speech. Participants were native English speakers (NS), and native Japanese speakers who spoke English as a second language (NNS). In Experiment 1, participants listened to "locally time-reversed speech" where every x-ms of speech signal was reversed on the temporal axis. Here, the local time reversal shifted the constituents of the speech signal forward or backward from the original position, and the amplitude envelope of speech was altered as a function of reversed segment length...
2018: Frontiers in Psychology
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