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https://www.readbyqxmd.com/read/29350722/chemical-decontamination-of-ips-cell-derived-neural-cell-mixtures
#1
Di Mao, Xie Khim Watson Chung, Tomoko Andoh-Noda, Ying Qin, Shin-Ichi Sato, Yasushi Takemoto, Wado Akamatsu, Hideyuki Okano, Motonari Uesugi
This report describes the design and evaluation of phosphorylated 7-ethyl-10-hydroxycamptothecin (SN38-P), which selectively eliminates tumor-forming proliferative stem cells, including human induced pluripotent stem cells (hiPSCs) and neural stem cells, from iPSC-derived neural cell mixtures. Results of the present study demonstrate that simple phosphorylation of an anticancer drug can provide a safe, cost-effective, and chemically-defined tool for decontaminating hiPSC-derived neuron.
January 19, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29349492/ganoderma-lucidum-phosphoglucomutase-is-required-for-hyphal-growth-polysaccharide-production-and-cell-wall-integrity
#2
Yanru Hu, Mengjiao Li, Shengli Wang, Sining Yue, Liang Shi, Ang Ren, Mingwen Zhao
Phosphoglucomutase (pgm) is an important enzyme in carbohydrate metabolism that is located at the branching point between glycolysis and the Leloir pathway. pgm catalyzes the reversible conversion reaction between glucose-6-phosphate (Glc-6-P) and glucose-1-phosphate (Glc-1-P). The glpgm gene was cloned in Escherichia coli, and the recombinant pgm protein from Ganoderma lucidum was purified in this study. The activity of native pgm was also detected to demonstrate that this predicted gene was functional in G...
January 18, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29343702/analysis-of-mitochondrial-function-in-human-induced-pluripotent-stem-cells-from-patients-with-mitochondrial-diabetes-due-to-the-a3243g-mutation
#3
Masaki Matsubara, Hajime Kanda, Hiromi Imamura, Mayumi Inoue, Michio Noguchi, Kiminori Hosoda, Akira Kakizuka, Kazuwa Nakao
We previously established human induced pluripotent stem (iPS) cells in two diabetic patients from different families with the mitochondrial A3243G mutation and isolated isogenic iPS cell clones with either undetectable or high levels of the mutation in both patients. In the present study, we analyzed the mitochondrial functions of two mutation-undetectable and two mutation-high clones in each patient through four methods to assess complex I activity, mitochondrial membrane potential, mitochondrial respiration, and mitochondrial ATP production...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29339073/the-human-gcom1-complex-gene-interacts-with-the-nmda-receptor-and-internexin-alpha
#4
Raymond S Roginski, Chi W Lau, Phillip P Santoiemma, Sara J Weaver, Peicheng Du, Patricia Soteropoulos, Jay Yang
The known functions of the human GCOM1 complex hub gene include transcription elongation and the intercalated disk of cardiac myocytes. However, in all likelihood, the gene's most interesting, and thus far least understood, roles will be found in the central nervous system. To investigate the functions of the GCOM1 gene in the CNS, we have cloned human and rat brain cDNAs encoding novel, 105 kDa GCOM1 combined (Gcom) proteins, designated Gcom15, and identified a new group of GCOM1 interacting genes, termed Gints, from yeast two-hybrid (Y2H) screens...
January 12, 2018: Gene
https://www.readbyqxmd.com/read/29338603/emerging-roles-of-inositol-pyrophosphates-as-key-modulators-of-fungal-pathogenicity
#5
Hyun Ah Kang
Inositol pyrophosphates (PP-IPs) are energy-rich small molecules that are omnipresent in eukaryotic cells, from yeast to mammals, playing central roles in overall cellular homeostasis as a diverse and multifaceted class of intracellular messengers. Recent studies of the metabolic pathways and physiological roles of PP-IPs in the human pathogenic fungus Cryptococcus neoformans have revealed that the PP-IP5 (IP7) is a key metabolite essential for fungal metabolic adaptation to the host environment, immune recognition, and pathogenicity...
January 17, 2018: Virulence
https://www.readbyqxmd.com/read/29336292/use-of-intraoperative-negative-margins-reduces-inverted-papilloma-recurrence
#6
Amar Miglani, Joseph M Hoxworth, Matthew A Zarka, Devyani Lal
BACKGROUND: Recurrence of inverted papilloma (IP) is a problem in 12-17% of tumors. Controversy exists regarding benefits of intraoperative frozen section histopathology (IFSH) for IP resection; however, to our knowledge, no study has specifically investigated this. IFSH for IP resection is the standard of care in our practice. We, therefore, reviewed our outcomes of using IFSH for IP resection. A secondary goal was to assess the reliability of IFSH. METHODS: Patients with IP who underwent surgical resection (2010-2016) with minimum 9-month follow-up were included...
January 1, 2018: American Journal of Rhinology & Allergy
https://www.readbyqxmd.com/read/29334878/the-use-of-standardized-discharge-in-ips-supported-employment-programs
#7
Justin Metcalfe, Robert E Drake, Gary Bond
OBJECTIVE: Some researchers have argued for using standardized discharge rules in individual placement and support (IPS) based on time of unemployment. To evaluate potential adverse outcomes of these rules, the authors examined time to first job over 24 months in a large randomized controlled study. METHODS: This secondary analysis of 2,055 participants in the Mental Health Treatment Study, using bootstrapping and survival analysis, estimated and compared the likelihood of finding a first job in the IPS and control groups during each quarter over 24 months...
January 16, 2018: Psychiatric Services: a Journal of the American Psychiatric Association
https://www.readbyqxmd.com/read/29334780/rules-governing-the-mechanism-of-epigenetic-reprogramming-memory
#8
Phuc-Loi Luu, Daniela Gerovska, Hans R Schöler, Marcos J Araúzo-Bravo
AIM: Disclosing the mechanisms that regulate reprogramming memory. MATERIALS & METHODS: We established computational procedure to find DNA methylation somatic memory sites (SMSs) at single CpGs and integrated them with genomics, epigenomics, transcriptomics and imprinting information. RESULTS & CONCLUSION: Reprogramming memory persists at late passages in low methylated regions. Contrarily to hypomethylated, hypermethylated SMSs occur at evolutionary conserved sites overlapping active transcription loci in dynamic chromatin regions...
January 16, 2018: Epigenomics
https://www.readbyqxmd.com/read/29327201/allele-specific-biased-expression-of-the-cntn6-gene-in-ips-cell-derived-neurons-from-a-patient-with-intellectual-disability-and-3p26-3-microduplication-involving-the-cntn6-gene
#9
Maria M Gridina, Natalia M Matveeva, Veniamin S Fishman, Aleksei G Menzorov, Helen A Kizilova, Nikolay A Beregovoy, Igor I Kovrigin, Inna E Pristyazhnyuk, Igor P Oscorbin, Maxim L Filipenko, Anna A Kashevarova, Nikolay A Skryabin, Tatyana V Nikitina, Elena A Sazhenova, Ludmila P Nazarenko, Igor N Lebedev, Oleg L Serov
Copy number variations (CNVs) of the human CNTN6 gene caused by megabase-scale microdeletions or microduplications in the 3p26.3 region are often the cause of neurodevelopmental disorders, including intellectual disability and developmental delay. Surprisingly, patients with different copy numbers of this gene display notable overlapping of neuropsychiatric symptoms. The complexity of the study of human neuropathologies is associated with the inaccessibility of brain material. This problem can be overcome through the use of reprogramming technologies that permit the generation of induced pluripotent stem (iPS) cells from fibroblasts and their subsequent in vitro differentiation into neurons...
January 11, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29324788/esrp1-is-a-marker-of-mouse-fetal-germ-cells-and-differentially-expressed-during-spermatogenesis
#10
Shaghayegh Saeidi, Farnaz Shapouri, Robb U de Iongh, Franca Casagranda, Jessie M Sutherland, Patrick S Western, Eileen A McLaughlin, Mary Familari, Gary R Hime
ESRP1 regulates alternative splicing, producing multiple transcripts from its target genes in epithelial tissues. It is upregulated during mesenchymal to epithelial transition associated with reprogramming of fibroblasts to iPS cells and has been linked to pluripotency. Mouse fetal germ cells are the founders of the adult gonadal lineages and we found that Esrp1 mRNA was expressed in both male and female germ cells but not in gonadal somatic cells at various stages of gonadal development (E12.5-E15.5). In the postnatal testis, Esrp1 mRNA was highly expressed in isolated cell preparations enriched for spermatogonia but expressed at lower levels in those enriched for pachytene spermatocytes and round spermatids...
2018: PloS One
https://www.readbyqxmd.com/read/29323782/the-effect-of-surface-treatments-on-the-mechanical-and-optical-behaviors-of-cad-cam-restorative-materials
#11
Sevcan Kurtulmus-Yilmaz, Esra Cengiz, Salim Ongun, Izgen Karakaya
PURPOSE: To evaluate the effect of different surface treatments on mechanical and optical properties of lithium disilicate ceramic (IPS e.max CAD), 2 resin nanoceramics (Lava Ultimate, GC Cerasmart), and polymer-infiltrated ceramic network material (Vita Enamic). MATERIALS AND METHODS: CAD/CAM blocks were sectioned into 4 × 1 × 14 mm3 bars for three-point bending test and 1 × 8 × 8 mm3 square specimens were obtained for color and translucency measurements. Bar-shaped and square specimens were divided into five groups according to the surface treatment method as: control (no treatment), hydrofluoric acid etching, airborne-particle abrasion, 2 W and 3 W Er,Cr:YSGG laser irradiation (n = 10)...
January 11, 2018: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/29321585/site-specific-randomization-of-the-endogenous-genome-by-a-regulatable-crispr-cas9-piggybac-system-in-human-cells
#12
Kentaro Ishida, Huaigeng Xu, Noriko Sasakawa, Mandy Siu Yu Lung, Julia Alexandra Kudryashev, Peter Gee, Akitsu Hotta
Randomized mutagenesis at an endogenous chromosomal locus is a promising approach for protein engineering, functional assessment of regulatory elements, and modeling genetic variations. In mammalian cells, however, it is challenging to perform site-specific single-nucleotide substitution with single-stranded oligodeoxynucleotide (ssODN) donor templates due to insufficient homologous recombination and the infeasibility of positive selection. Here, we developed a DNA transposon based CRISPR-Cas9 regulated transcription and nuclear shuttling (CRONUS) system that enables the stable transduction of CRISPR-Cas9/sgRNA in broad cell types, but avoids undesired genome cleavage in the absence two chemical inducing molecules...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321255/multisite-emergency-department-inpatient-collaborative-to-reduce-unnecessary-bronchiolitis-care
#13
Grant M Mussman, Michele Lossius, Faiza Wasif, Jeffrey Bennett, Kristin A Shadman, Susan C Walley, Lauren Destino, Elizabeth Nichols, Shawn L Ralston
BACKGROUND AND OBJECTIVES: There is high variation in the care of acute viral bronchiolitis. We sought to promote collaboration between emergency department (ED) and inpatient (IP) units with the goal of reducing unnecessary testing and treatment. METHODS: Multisite collaborative with improvement teams co-led by ED and IP physicians and a 1-year period of active participation. The intervention consisted of a multicomponent change package, regular webinars, and optional coaching...
January 10, 2018: Pediatrics
https://www.readbyqxmd.com/read/29321010/evaluation-of-five-cad-cam-materials-by-microstructural-characterization-and-mechanical-tests-a-comparative-in-vitro-study
#14
Nesrin Sonmez, Pinar Gultekin, Volkan Turp, Gokhan Akgungor, Deniz Sen, Eitan Mijiritsky
BACKGROUND: Polymer infiltrated ceramics and nano-ceramic resins are the new restorative materials which have been developed in order to enhance the adverse properties of glass-matrix ceramics and resin composites. The aim of the present in vitro study was to evaluate the characteristics of various CAD/CAM materials through mechanical, microstructural, and SEM analysis. METHODS: Five test groups (n = 22) were formed by using the indicated CAD/CAM blocks: VITA Enamic (VITA Zahnfabrik), Lava Ultimate (3 M ESPE), IPS e...
January 8, 2018: BMC Oral Health
https://www.readbyqxmd.com/read/29320737/genomic-circuitry-underlying-immunological-response-to-pediatric-acute-respiratory-infection
#15
Sarah E Henrickson, Sasikanth Manne, Douglas V Dolfi, Kathleen D Mansfield, Kaela Parkhouse, Rakesh D Mistry, Elizabeth R Alpern, Scott E Hensley, Kathleen E Sullivan, Susan E Coffin, E John Wherry
Acute respiratory tract viral infections (ARTIs) cause significant morbidity and mortality. CD8 T cells are fundamental to host responses, but transcriptional alterations underlying anti-viral mechanisms and links to clinical characteristics remain unclear. CD8 T cell transcriptional circuitry in acutely ill pediatric patients with influenza-like illness was distinct for different viral pathogens. Although changes included expected upregulation of interferon-stimulated genes (ISGs), transcriptional downregulation was prominent upon exposure to innate immune signals in early IFV infection...
January 9, 2018: Cell Reports
https://www.readbyqxmd.com/read/29318304/prediction-of-physico-chemical-properties-of-bacteriostatic-n1-substituted-sulfonamides-theoretical-and-experimental-studies
#16
Hossein Nikoofard, Mohsen Sargolzaei, Farnosh Faridbod
A computational study at the density functional theory (DFT) as well as electrochemical methods, was carried out on the structural and physico-chemical properties of a series of sulfonamide derivatives (SAs) as WHO essential medications in the treatment of basic health system. The B3LYP/6-311++G(d,p) level of theory carried out on sulfadiazine (SDZ), sulfathiazole (STZ), sulfaquinoxaline (SQX), sulfacetamide (SAA), and the reference unsubstituted sulfonamide (SA) was discussed and rationalized in term of the N1-sulfonamide substituent...
December 2017: Acta Chimica Slovenica
https://www.readbyqxmd.com/read/29316596/van-neck-odelberg-disease-a-3-5-year-follow-up-case-report-and-systematic-review
#17
Patrick J Mixa, Frank A Segreto, Hiram Luigi-Martinez, Bassel G Diebo, Qais Naziri, Srinivas Kolla, Aditya V Maheshwari
V an Neck-Odelberg disease (VND) is a benign skeletal overgrowth of the ischiopubic synchondrosis (IPS) in prepubescent patients. There is a paucity of long-term follow-up data and reviews on management decision-making. We report on a 15-year-old female, with a history of sickle-cell disease (HbSS), presenting with unilateral groin pain. Patient's physical examination, radiographs, and a literature-review determined a diagnosis of VND. Conservative treatment was issued. Clinical symptoms resolved at three months, followed by complete lesion resolution at three years...
December 12, 2017: Surgical Technology International
https://www.readbyqxmd.com/read/29315221/introduction-of-exogenous-hsv-tk-suicide-gene-increases-safety-of-keratinocyte-derived-induced-pluripotent-stem-cells-by-providing-genetic-emergency-exit-switch
#18
Maciej Sułkowski, Paweł Konieczny, Paula Chlebanowska, Marcin Majka
Since their invention in 2006, induced Pluripotent Stem (iPS) cells remain a great promise for regenerative medicine circumventing the ethical issues linked to Embryonic Stem (ES) cell research. iPS cells can be generated in a patient-specific manner as an unlimited source of various cell types for in vitro drug screening, developmental biology studies and regenerative use. Having the capacity of differentiating into the cells of all three primary germ layers, iPS cells have high potential to form teratoma tumors...
January 9, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29314433/the-effect-of-sintering-time-on-the-marginal-fit-of-zirconia-copings
#19
Amir Ali Reza Khaledi, Mahroo Vojdani, Mitra Farzin, Sudabeh Pirouzi, Sepideh Orandi
PURPOSE: This study analyzed the effect of different sintering times on the marginal adaptation of zirconia ceramic copings. MATERIALS AND METHODS: Thirty copings of pre-sintered 3Y-TZP blanks were milled and subsequently sintered in a special furnace for three different durations (n = 10 per group). The sintering time was 1 hour, 15 minutes for IPS e.max ZirCAD, 4 hours 20 minutes for Speed ZrO2, and 7 hours 20 minutes for the conventional ZrO2 group. The marginal gaps of specimens were measured at 18 spots on the master die by employing a digital microscope...
January 3, 2018: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/29312817/murine-pluripotent-stem-cells-that-escape-differentiation-inside-teratomas-maintain-pluripotency
#20
Yangli Pei, Liang Yue, Wei Zhang, Jinzhu Xiang, Zhu Ma, Jianyong Han
Background: Pluripotent stem cells (PSCs) offer immense potential as a source for regenerative therapies. The teratoma assay is widely used in the field of stem cells and regenerative medicine, but the cell composition of teratoma is still elusive. Methods: We utilized PSCs expressing enhanced green fluorescent protein (EGFP) under the control of the Pou5f1 promoter to study the persistence of potential pluripotent cells during teratoma formation in vivo. OCT4-MES (mouse embryonic stem cells) were isolated from the blastocysts of 3...
2018: PeerJ
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