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https://www.readbyqxmd.com/read/28345153/the-functional-architectures-of-addition-and-subtraction-network-discovery-using-fmri-and-dcm
#1
Yang Yang, Ning Zhong, Karl Friston, Kazuyuki Imamura, Shengfu Lu, Mi Li, Haiyan Zhou, Haiyuan Wang, Kuncheng Li, Bin Hu
The neuronal mechanisms underlying arithmetic calculations are not well understood but the differences between mental addition and subtraction could be particularly revealing. Using fMRI and dynamic causal modeling (DCM), this study aimed to identify the distinct neuronal architectures engaged by the cognitive processes of simple addition and subtraction. Our results revealed significantly greater activation during subtraction in regions along the dorsal pathway, including the left inferior frontal gyrus (IFG), middle portion of dorsolateral prefrontal cortex (mDLPFC), and supplementary motor area (SMA), compared with addition...
March 27, 2017: Human Brain Mapping
https://www.readbyqxmd.com/read/28343087/cad-cam-machining-vs-pre-sintering-in-lab-fabrication-techniques-of-y-tzp-ceramic-specimens-effects-on-their-mechanical-fatigue-behavior
#2
C P Zucuni, L F Guilardi, S Fraga, L G May, G K R Pereira, L F Valandro
This study evaluated the effects of different pre-sintering fabrication processing techniques of Y-TZP ceramic (CAD/CAM Vs. in-lab), considering surface characteristics and mechanical performance outcomes. Pre-sintered discs of Y-TZP ceramic (IPS e.max ZirCAD, Ivoclar Vivadent) were produced using different pre-sintering fabrication processing techniques: Machined- milling with a CAD/CAM system; Polished- fabrication using a cutting device followed by polishing (600 and 1200 SiC papers); Xfine- fabrication using a cutting machine followed by grinding with extra-fine diamond bur (grit size 30 μm); Fine- fabrication using a cutting machine followed by grinding with fine diamond bur (grit size 46 μm); SiC- fabrication using a cutting machine followed by grinding with 220 SiC paper...
March 18, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28341165/the-left-intraparietal-sulcus-adapts-to-symbolic-number-in-both-the-visual-and-auditory-modalities-evidence-from-fmri
#3
Stephan E Vogel, Celia Goffin, Joshua Bohnenberger, Karl Koschutnig, Gernot Reishofer, Roland H Grabner, Daniel Ansari
A growing body of evidence from functional Magnetic Resonance Imaging adaptation (fMRIa) has implicated the left intraparietal sulcus (IPS) as a crucial brain region representing the semantic meaning of number symbols. However, it is currently unknown to what extent the left IPS brain activity can be generalized across modalities (e.g., Arabic digits and spoken number words) and how robust and reproducible numerical adaptation effects are. In two separate fMRIa experiments we habituated the brain response of 20 native English-speaking (Experiment 1) and 34 native German-speaking (Experiment 2) adults to Arabic digits or spoken number words...
March 21, 2017: NeuroImage
https://www.readbyqxmd.com/read/28341161/predictive-position-computations-mediated-by-parietal-areas-tms-evidence
#4
Grace Edwards, Céline Paeye, Philippe Marque, Rufin VanRullen, Patrick Cavanagh
When objects move or the eyes move, the visual system can predict the consequence and generate a percept of the target at its new position. This predictive localization may depend on eye movement control in the frontal eye fields (FEF) and the intraparietal sulcus (IPS) and on motion analysis in the medial temporal area (MT). Across two experiments we examined whether repetitive transcranial magnetic stimulation (rTMS) over right FEF, right IPS, right MT, and a control site, peripheral V1/V2, diminished participants' perception of two cases of predictive position perception: trans-saccadic fusion, and the flash grab illusion, both presented in the contralateral visual field...
March 21, 2017: NeuroImage
https://www.readbyqxmd.com/read/28341059/masking-properties-of-ceramics-for-veneer-restorations
#5
Andreas L Skyllouriotis, Hideo L Yamamoto, Dan Nathanson
STATEMENT OF PROBLEM: The translucency and opacity of ceramics play a significant role in emulating the natural color of teeth, but studies of the masking properties and limitations of dental ceramics when used as monolayer restorations are lacking. PURPOSE: The purpose of this in vitro study was to determine the translucency of 6 materials used for veneer restorations by assessing their translucency parameters (TPs), contrast ratios (CRs), and potential to mask dark tooth colors...
March 21, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28340154/irradiation-strongly-reduces-tumorigenesis-of-human-induced-pluripotent-stem-cells
#6
Shoki Inui, Kazumasa Minami, Emiko Ito, Hiromasa Imaizumi, Seiji Mori, Masahiko Koizumi, Satsuki Fukushima, Shigeru Miyagawa, Yoshiki Sawa, Nariaki Matsuura
Induced pluripotent stem (iPS) cells have demonstrated they can undergo self-renewal, attain pluripotency, and differentiate into various types of functional cells. In clinical transplantation of iPS cells, however, a major problem is the prevention of tumorigenesis. We speculated that tumor formation could be inhibited by means of irradiation. Since the main purpose of this study was to explore the prevention of tumor formation in human iPS (hiPS) cells, we tested the effects of irradiation on tumor-associated factors such as radiosensitivity, pluripotency and cell death in hiPS cells...
March 3, 2017: Journal of Radiation Research
https://www.readbyqxmd.com/read/28339300/genetic-manipulation-by-zinc-finger-nucleases-in-rat-induced-pluripotent-stem-cells
#7
Sheng Yang, Shufang Ding, Qianhua Xu, Xiong Li, Qiong Xiong
Induced pluripotent stem cells (iPSCs) have an extensive application in regenerative medicine, pharmaceutical discovery, and basic research. With the recent derivation of rat iPSCs, it is now feasible to apply genetic manipulation in this species. But such tools do not yet exist for many rat strains, especially for disease model rat. The Sprague Dawley (SD) rat is an inbred disease model for hypertension, nephropathy, pulmonary hypertension, depression, and alcohol consumption. In this study, the SD rat iPSCs were generated using lentiviral method...
March 24, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28338936/establishment-of-a-human-hepatocellular-cell-line-capable-of-maintaining-long-term-replication-of-hepatitis-b-virus
#8
Wan-Ling Yao, Sotaro Ikeda, Yuta Tsukamoto, Keiko Shindo, Yukie Otakaki, Main Qin, Yoshikazu Iwasawa, Fumihiko Takeuchi, Yuki Kaname, Yu-Chi Chou, Chungming Chang, Koichi Watashi, Takaji Wakita, Takeshi Noda, Hiroki Kato, Takashi Fujita
Hepatitis B virus is a virus whose replication cycle cannot be completely reproduced using cultured cell lines. Here, we report an engineered cell line capable of supporting the complete HBV life cycle. We generated HepG2 cells overexpressing HBV entry receptor human NTCP, and defective in RIG-I-like receptor signaling, by knocking down the IPS-1 adaptor molecule. The resultant NtG20.i7 cells were susceptible to HBV, and its replication was detectable at 14 days post-infection and persisted for at least 35 days with a gradual increase of HBc expression...
March 11, 2017: International Immunology
https://www.readbyqxmd.com/read/28336435/in%C3%A2-vitro-model-of-cerebral-ischemia-by-using-brain-microvascular-endothelial-cells-derived-from-human-induced-pluripotent-stem-cells
#9
Yasuhiro Kokubu, Tomoko Yamaguchi, Kenji Kawabata
Brain-derived microvascular endothelial cells (BMECs), which play a central role in blood brain barrier (BBB), can be used for the evaluation of drug transport into the brain. Although human BMEC cell lines have already been reported, they lack original properties such as barrier integrity. Pluripotent stem cells (PSCs) can be used for various applications such as regenerative therapy, drug screening, and pathological study. In the recent study, an induction method of BMECs from PSCs has been established, making it possible to more precisely study the in vitro human BBB function...
March 21, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28335032/allele-specific-ablation-rescues-electrophysiological-abnormalities-in-a-human-ips-cell-model-of-long-qt-syndrome-with-a-calm2-mutation
#10
Yuta Yamamoto, Takeru Makiyama, Takeshi Harita, Kenichi Sasaki, Yimin Wuriyanghai, Mamoru Hayano, Suguru Nishiuchi, Hirohiko Kohjitani, Sayako Hirose, Jiarong Chen, Fumika Yokoi, Taisuke Ishikawa, Seiko Ohno, Kazuhisa Chonabayashi, Hideki Motomura, Yoshinori Yoshida, Minoru Horie, Naomasa Makita, Takeshi Kimura
Background: Calmodulin is a ubiquitous Ca 2+ sensor molecule encoded by three distinct calmodulin genes, CALM1-3 . Recently, mutations in CALM1-3 have been reported to be associated with severe early-onset long-QT syndrome (LQTS). However, the underlying mechanism through which heterozygous calmodulin mutations lead to severe LQTS remains unknown, particularly in human cardiomyocytes. Objectives: We aimed to establish an LQTS disease model associated with a CALM2 mutation (LQT15) using human induced pluripotent stem cells (hiPSCs) and to assess mutant allele-specific ablation by genome editing for the treatment of LQT15...
March 1, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28334759/highly-efficient-biallelic-genome-editing-of-human-es-ips-cells-using-a-crispr-cas9-or-talen-system
#11
Kazuo Takayama, Keisuke Igai, Yasuko Hagihara, Rina Hashimoto, Morifumi Hanawa, Tetsushi Sakuma, Masashi Tachibana, Fuminori Sakurai, Takashi Yamamoto, Hiroyuki Mizuguchi
Genome editing research of human ES/iPS cells has been accelerated by clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) and transcription activator-like effector nucleases (TALEN) technologies. However, the efficiency of biallelic genetic engineering in transcriptionally inactive genes is still low, unlike that in transcriptionally active genes. To enhance the biallelic homologous recombination efficiency in human ES/iPS cells, we performed screenings of accessorial genes and compounds...
February 21, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28332899/in-vitro-tests-for-aerosol-deposition-v-using-realistic-testing-to-estimate-variations-in-aerosol-properties-at-the-trachea
#12
Xiangyin Wei, Michael Hindle, Renishkumar R Delvadia, Peter R Byron
BACKGROUND: The dose and aerodynamic particle size distribution (APSD) of drug aerosols' exiting models of the mouth and throat (MT) during a realistic inhalation profile (IP) may be estimated in vitro and designated Total Lung Dose, TLDin vitro, and APSDTLDin vitro, respectively. These aerosol characteristics likely define the drug's regional distribution in the lung. METHODS: A general method was evaluated to enable the simultaneous determination of TLDin vitro and APSDTLDin vitro for budesonide aerosols' exiting small, medium and large VCU-MT models...
March 23, 2017: Journal of Aerosol Medicine and Pulmonary Drug Delivery
https://www.readbyqxmd.com/read/28332572/xeno-free-culture-of-human-pluripotent-stem-cells-on-oligopeptide-grafted-hydrogels-with-various-molecular-designs
#13
Yen-Ming Chen, Li-Hua Chen, Meng-Pei Li, Hsing-Fen Li, Akon Higuchi, S Suresh Kumar, Qing-Dong Ling, Abdullah A Alarfaj, Murugan A Munusamy, Yung Chang, Giovanni Benelli, Kadarkarai Murugan, Akihiro Umezawa
Establishing cultures of human embryonic (ES) and induced pluripotent (iPS) stem cells in xeno-free conditions is essential for producing clinical-grade cells. Development of cell culture biomaterials for human ES and iPS cells is critical for this purpose. We designed several structures of oligopeptide-grafted poly (vinyl alcohol-co-itaconic acid) hydrogels with optimal elasticity, and prepared them in formations of single chain, single chain with joint segment, dual chain with joint segment, and branched-type chain...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28332333/allo-hla-cross-reactivities-of-cmv-flu-and-vzv-specific-memory-t-cells-are-shared-by-different-individuals
#14
H van den Heuvel, K M Heutinck, E M W van der Meer-Prins, S L Yong, P P M C van Miert, J D H Anholts, M E I Franke-van Dijk, X Q Zhang, D L Roelen, R J M Ten Berge, F H J Claas
Virus-specific T cells can recognize allogeneic HLA (allo-HLA) through TCR cross-reactivity. The allospecificity often differs per individual ("private cross-reactivity"), but can also be shared by multiple individuals ("public cross-reactivity"). However, only a few examples of the latter have been described. Since these could facilitate alloreactivity prediction in transplantation, we aimed to identify novel public cross-reactivities of human virus-specific CD8+ T cells directed against allo-HLA by assessing their reactivity in mixed-lymphocyte reactions...
March 23, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28326803/when-is-an-alveolar-type-2-cell-an-alveolar-type-2-cell-a-conundrum-for-lung-stem-cell-biology-and-regenerative-medicine
#15
Michael F Beers, Yuben Moodley
Generating mature, differentiated, adult lung cells from pluripotent cells such as induced pluripotent cells (iPS) and embryonic stem cells (ES) offers the hope of both generating disease specific in vitro models and creating definitive and personalized therapies for a host of debilitating lung parenchymal and airway diseases. With the goal of advancing lung regenerative medicine, several groups have developed and reported on protocols utilizing either defined media, co-culture with mesenchymal components, or sequential treatments mimicking lung development, to obtain distal lung epithelial cells from stem cell precursors...
March 22, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28326726/-effects-of-different-surface-modifications-on-micro-structure-and-adhesion-of-zirconia-ceramic-an-in-vitro-study
#16
Li Siwen, Li Shishi, Wang Yanhong, Ma Hongmei
OBJECTIVE: This study evaluated the effect of different mechanical-chemical surface treatments on the characteristics, microstructure, and composition of zirconia ceramics and on the zirconia-dentin adhesion. METHODS: The sintered commercial zirconia blocks (IPS e.max ZirCAD) were sectioned into 126 beams (6 mm×6 mm×5 mm) and randomly assigned to seven experimental groups (n=18). The zirconia block specimens were further treated as follows: (A) untreated, as control; (B) sandblasted with 50 μm Al2O3; (C) sandblasted with 50 μm Al2O3+30 μm silica powder; (D) sandblasted with 50 μm Al2O3+30% silica-sol coating; (E) sandblasted with 110 μm Al2O3; (F) sandblasted with 110 μm Al2O3+30 μm silica powder; and (G) sandblasted with 110 μm Al2O3+30% silica-sol coating...
February 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28326324/three-dimensional-human-cardiac-tissue-engineered-by-centrifugation-of-stacked-cell-sheets-and-cross-sectional-observation-of-its-synchronous-beatings-by-optical-coherence-tomography
#17
Yuji Haraguchi, Akiyuki Hasegawa, Katsuhisa Matsuura, Mari Kobayashi, Shin-Ichi Iwana, Yasuhiro Kabetani, Tatsuya Shimizu
Three-dimensional (3D) tissues are engineered by stacking cell sheets, and these tissues have been applied in clinical regenerative therapies. The optimal fabrication technique of 3D human tissues and the real-time observation system for these tissues are important in tissue engineering, regenerative medicine, cardiac physiology, and the safety testing of candidate chemicals. In this study, for aiming the clinical application, 3D human cardiac tissues were rapidly fabricated by human induced pluripotent stem (iPS) cell-derived cardiac cell sheets with centrifugation, and the structures and beatings in the cardiac tissues were observed cross-sectionally and noninvasively by two optical coherence tomography (OCT) systems...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28324490/assessment-of-mitophagy-in-ips-cell-derived-neurons
#18
Kei-Ichi Ishikawa, Akihiro Yamaguchi, Hideyuki Okano, Wado Akamatsu
Aberrant mitochondrial function is associated with many neurological diseases. Mitophagy is a key mechanism for the elimination of damaged mitochondria and maintenance of mitochondrial homeostasis. Induced pluripotent stem (iPS) cell technologies developed over the last decade have allowed us to analyze functions of the human neuron. Here we describe an efficient induction method from human iPS cells to neurons, followed by an image-based mitophagy assay.
March 22, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28322920/stem-cell-transplantation-therapy-for-multifaceted-therapeutic-benefits-after-stroke
#19
REVIEW
Ling Wei, Zheng Z Wei, Michael Qize Jiang, Osama Mohamad, Shan Ping Yu
One of the exciting advances in modern medicine and life science is cell-based neurovascular regeneration of damaged brain tissues and repair of neuronal structures. The progress in stem cell biology and creation of adult induced pluripotent stem (iPS) cells has significantly improved basic and pre-clinical research in disease mechanisms and generated enthusiasm for potential applications in the treatment of central nervous system (CNS) diseases including stroke. Endogenous neural stem cells and cultured stem cells are capable of self-renewal and give rise to virtually all types of cells essential for the makeup of neuronal structures...
March 16, 2017: Progress in Neurobiology
https://www.readbyqxmd.com/read/28321551/top-down-cortical-interactions-in-visuospatial-attention
#20
Timothy P Meehan, Steven L Bressler, Wei Tang, Serguei V Astafiev, Chad M Sylvester, Gordon L Shulman, Maurizio Corbetta
The voluntary allocation of visuospatial attention depends upon top-down influences from the frontal eye field (FEF) and intraparietal sulcus (IPS)-the core regions of the dorsal attention network (DAN)-to visual occipital cortex (VOC), and has been further associated with within-DAN influences, particularly from the FEF to IPS. However, the degree to which these influences manifest at rest and are then modulated during anticipatory visuospatial attention tasks remains poorly understood. Here, we measured both undirected and directed functional connectivity (UFC, DFC) between the FEF, IPS, and VOC at rest and during an anticipatory visuospatial attention task, using a slow event-related design...
March 20, 2017: Brain Structure & Function
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