keyword
MENU ▼
Read by QxMD icon Read
search

iPS

keyword
https://www.readbyqxmd.com/read/29789608/downregulation-of-endothelin-receptor-b-contributes-to-defective-b-cell-lymphopoiesis-in-trisomy-21-pluripotent-stem-cells
#1
Glenn A MacLean, Jennifer McEldoon, Jialiang Huang, Jeremy Allred, Matthew C Canver, Stuart H Orkin
Individuals with Trisomy 21 (T21) exhibit numerous hematological abnormalities, including reductions in numbers of circulating B and T lymphocytes. To elucidate molecular mechanisms underlying these phenotypes, we differentiated human isogenic disomic and trisomic pluripotent cells, and observed that trisomic cells showed defects in B cell, but not T cell differentiation. Global gene expression of differentiated, trisomic B cells revealed reduced expression of genes encoding endothelin signaling components, namely the Endothelin Receptor B (EDNRB), and its ligand Endothelin1 (EDN1)...
May 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29781803/tendency-of-k562-chronic-myeloid-leukemia-cells-towards-cell-reprogramming
#2
Açelya Yılmazer Aktuna
Cancer cell reprogramming is a potential tool to study cancer progression, disease pathology and drug sensitivity. Prior to performing cancer reprogramming studies, it is important to evaluate the stemness predisposition of cells that will be reprogrammed. Here, we performed a proof-of-concept study with chronic myeloid leukemia K562 cells in order to evaluate their tendency for cancer cell reprogramming. Expression of reprogramming factors, pluripotency markers and tumor-suppressor genes were analyzed at gene and protein level via real-time RT-PCR and flow cytometry...
May 21, 2018: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
https://www.readbyqxmd.com/read/29780384/discrimination-between-human-leukocyte-antigen-class-i-bound-and-co-purified-hiv-derived-peptides-in-immunopeptidomics-workflows
#3
Thomas Partridge, Annalisa Nicastri, Anna E Kliszczak, Louis-Marie Yindom, Benedikt M Kessler, Nicola Ternette, Persephone Borrow
Elucidation of novel peptides presented by human leukocyte antigen (HLA) class I alleles by immunopeptidomics constitutes a powerful approach that can inform the rational design of CD8+ T cell inducing vaccines to control infection with pathogens such as human immunodeficiency virus type 1 (HIV-1) or to combat tumors. Recent advances in the sensitivity of liquid chromatography tandem mass spectrometry instrumentation have facilitated the discovery of thousands of natural HLA-restricted peptides in a single measurement...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29780042/long-term-culture-of-human-ips-cell-derived-telencephalic-neuron-aggregates-on-collagen-gel
#4
Hiroshi Oyama, Koji Takahashi, Yoshikazu Tanaka, Hiroshi Takemoto, Hisashi Haga
It takes several months to form the 3-dimensional morphology of the human embryonic brain. Therefore, establishing a long-term culture method for neuronal tissues derived from human induced pluripotent stem (iPS) cells is very important for studying human brain development. However, it is difficult to keep primary neurons alive for more than 3 weeks in culture. Moreover, long-term adherent culture to maintain the morphology of telencephalic neuron aggregates induced from human iPS cells is also difficult. Although collagen gel has been widely used to support long-term culture of cells, it is not clear whether human iPS cell-derived neuron aggregates can be cultured for long periods on this substrate...
2018: Cell Structure and Function
https://www.readbyqxmd.com/read/29779282/-effect-of-bone-morphogenetic-protein-4-overexpression-on-the-biological-activity-of-mouse-induced-pluripotent-stem-cells
#5
Jin Qiu, Hui-Yu Zhang, Li Liu, Ying-Hui Tan
OBJECTIVE: This study aimed to construct the expression of bone morphogenetic protein-4 (BMP4) lentiviral vector gene and explore its influence on the biological activity of mouse induced pluripotent stem (iPS) cells. METHODS: iPS cell lines stably overexpressing BMP4 were constructed by lentivirus transfection (BMP4-overexpressing group). Cells without transfection served as the blank group, and cells with only vector transfection served as the empty-vector group...
April 1, 2018: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29778029/late-onset-childhood-neuronal-ceroid-lipofuscinosis-early-clinical-and-electroencephalographic-markers
#6
Lucas Beltrán, Gabriela Reyes Valenzuela, Mariana Loos, Rodrigo Vargas, Rafael Lizama, Pablo Spinsanti, Roberto Caraballo
PURPOSE: The objective of the study was to describe the initial clinical and electroencephalographic findings in children with late-infantile neuronal ceroid lipofuscinosis (LINCL). METHOD: The clinical charts of 35 patients seen between 1990 and 2016 were reviewed. The patients were divided into two groups: Group 1 (G1) consisting of 12 patients with NCL type 2 (CLN2) disease confirmed by enzymatic activity in dried blood spots on filter paper and/or genetic studies, and Group 2 (G2) consisting of 23 patients with a diagnosis of LINCL based on pathology studies by muscle biopsy...
May 15, 2018: Epilepsy Research
https://www.readbyqxmd.com/read/29777485/co-suppression-of-atmips-demonstrates-cooperation-of-mips1-mips2-and-mips3-in-maintaining-myo-inositol-synthesis
#7
C M Fleet, J Y Yen, E A Hill, G E Gillaspy
Co-suppressed MIPS2 transgenic lines allow bypass of the embryo lethal phenotype of the previously published triple knock-out and demonstrate the effects of MIPS on later stages of development. Regulation of inositol production is of interest broadly for its effects on plant growth and development. The enzyme L-myo-inositol 1-phosphate synthase (MIPS, also known as IPS) isomerizes D-glucose-6-P to D-inositol 3-P, and this is the rate-limiting step in inositol production. In Arabidopsis thaliana, the MIPS enzyme is encoded by three different genes, (AtMIPS1, AtMIPS2 and AtMIPS3), each of which has been shown to produce proteins with biochemically similar properties but differential expression patterns...
May 18, 2018: Plant Molecular Biology
https://www.readbyqxmd.com/read/29776436/rates-and-causes-of-30-day-readmission-and-emergency-room-utilization-following-head-and-neck-surgery
#8
Vincent Wu, Stephen F Hall
BACKGROUND: Unplanned returns to hospital are common, costly, and potentially avoidable. We aimed to investigate and characterize reasons for all-cause readmissions to hospital as in-patients (IPs) and visits to the Emergency Department (ED) within 30-days following patient discharge post head and neck surgery (HNS). METHODS: Retrospective case series with chart review. All patients within the Department of Otolaryngology - Head and Neck Surgery who underwent HNS for benign and malignant disease from January 1, 2010 to May 31, 2015 were identified...
May 18, 2018: Journal of Otolaryngology—Head & Neck Surgery
https://www.readbyqxmd.com/read/29775409/transactivation-domain-of-p53-regulates-dna-repair-and-integrity-in-human-ips-cells
#9
Ramaswamy Kannappan, Saidulu Mattapally, Pooja A Wagle, Jianyi Zhang
The role of p53 transactivation domain (p53-TAD), a multifunctional and dynamic domain, on DNA repair and retaining DNA integrity in human iPS cells has never been studied. p53-TAD was knocked out in iPS cells using CRISPR/Cas9 and was confirmed by DNA sequencing. p53-TAD KO cells were characterized by: accelerated proliferation, decreased population doubling time, and unaltered Bcl2, BBC3, IGF1R, Bax and altered Mdm2, p21, and PIDD transcripts expression. In p53-TAD KO cells p53 regulated DNA repair proteins XPA, DNA polH and DDB2 expression were found to be reduced compared to p53-WT cells...
May 18, 2018: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29770877/polishing-effects-and-wear-performance-of-chairside-cad-cam-materials
#10
Mike Matzinger, Sebastian Hahnel, Verena Preis, Martin Rosentritt
OBJECTIVES: To investigate the surface roughness of CAD/CAM materials immediately after milling and after different chairside and labside polishing procedures. A two-body wear test was performed to compare the different wear characteristics of the materials and the corresponding antagonists. MATERIALS AND METHODS: Specimens (n = 12 per series) from different CAD/CAM materials (three composites: Lava Ultimate, Cerasmart, BRILLIANT Crios; one hybrid ceramic: VITA Enamic; three ceramics: Celtra Duo, VITA Suprinity, IPS Emax...
May 16, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29755374/altered-amygdala-resting-state-functional-connectivity-and-hemispheric-asymmetry-in-patients-with-social-anxiety-disorder
#11
Ye-Ha Jung, Jung E Shin, Yoonji I Lee, Joon H Jang, Hang J Jo, Soo-Hee Choi
Background: The amygdala plays a key role in emotional hyperreactivity in response to social threat in patients with social anxiety disorder (SAD). We investigated resting-state functional connectivity (rs-FCN) of the left and right amygdala with various brain regions and functional lateralization in patients with SAD. Methods: A total of 36 patients with SAD and 42 matched healthy controls underwent functional magnetic resonance imaging (fMRI) at rest. Using the left and right amygdala as seed regions, we compared the strength of the rs-FCN in the patient and control groups...
2018: Frontiers in Psychiatry
https://www.readbyqxmd.com/read/29753274/blood-derived-integration-free-ips-cell-line-ukbi011-a-from-a-diagnosed-male-alzheimer-s-disease-patient-with-apoe-%C3%A9-4-%C3%A9-4-genotype
#12
Michael Peitz, Tamara Bechler, Catrin Cornelia Thiele, Monika Veltel, Melanie Bloschies, Klaus Fliessbach, Alfredo Ramirez, Oliver Brüstle
Alzheimer's disease (AD) is most the frequent neurodegenerative disease, and the APOE ε4 allele is the most prominent risk factor for late-onset AD. Here, we present an iPSC line generated from peripheral blood cells of a male AD patient employing Sendai virus vectors encoding the transcription factors OCT4, SOX2, KLF4 and c-MYC. The characterized iPSC line expresses typical human pluripotency markers and shows differentiation into all three germ layers, complete reprogramming vector clearance, a normal SNP genotype and maintenance of the APOE ε4/ε4 allele...
April 23, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29752542/a-computational-investigation-on-the-antioxidant-potential-of-myricetin-3-4-di-o-%C3%AE-l-rhamnopyranoside
#13
Rodrigo A Mendes, Shawan K C Almeida, Iuri N Soares, Cristina A Barboza, Renato G Freitas, Alex Brown, Gabriel L C de Souza
In this work, we present a computational study on the antioxidant potential of myricetin 3,4[Formula: see text]-di-O-α-L-rhamnopyranoside (Compound M). A density functional theory (DFT) approach with the B3LYP and LC-ωPBE functionals and with both the 6-311G(d,p) and 6-311+G(d,p) basis sets was used. The focus of the investigation was on the structural and energetic parameters including both bond dissociation enthalpies (BDEs) and ionization potentials (IPs), which provide information on the potential antioxidant activity...
May 11, 2018: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/29750664/using-illness-perceptions-to-cluster-chronic-pain-patients-results-from-a-trial-on-the-chronic-pain-self-management-program
#14
Lisbeth Frostholm, Christina Hornemann, Eva Ørnbøl, Per Fink, Mimi Mehlsen
OBJECTIVES: The aims of our study were (1) To identify possible subgroups of chronic pain sufferers based on their illness perceptions (IPs); (2) To examine whether these subgroups differed in health status and health expenditure, and (3) To examine whether the subgroups differed in their response to participation in a lay-led Chronic Pain Self-Management Program (CPSMP). METHODS: Four hundred and twenty-four participants in a randomized controlled trial on the CPSMP completed a questionnaire on their perceptions of their chronic pain condition at baseline...
May 10, 2018: Clinical Journal of Pain
https://www.readbyqxmd.com/read/29750232/in-vitro-evaluation-of-bond-strength-of-four-self-etching-cements
#15
Julio Chávez-Lozada, Carmen Urquía-Morales
Indirect restorations need to be attached with adhesive luting agents to prevent them from becoming dislodged and provide adequate marginal sealing. The aim of this study was to evaluate bond strength to dentin of self-etching resin cements. MATERIALS AND METHODS: 75 flat dentin surfaces were randomly distributed among 5 groups, according to cements used; Group 1: RelyxU100 (3M/ESPE), Group 2: Bis Cem (Bisco), Group 3: Max Cem (Kerr), Group 4: SeT PP (SDI) and Group 5: Relyx ARC (3M/ESPE), control...
December 2017: Acta Odontológica Latinoamericana: AOL
https://www.readbyqxmd.com/read/29748503/an-rfid-indoor-positioning-algorithm-based-on-support-vector-regression
#16
He Xu, Manxing Wu, Peng Li, Feng Zhu, Ruchuan Wang
Nowadays, location-based services, which include services to identify the location of a person or an object, have many uses in social life. Though traditional GPS positioning can provide high quality positioning services in outdoor environments, due to the shielding of buildings and the interference of indoor environments, researchers and enterprises have paid more attention to how to perform high precision indoor positioning. There are many indoor positioning technologies, such as WiFi, Bluetooth, UWB and RFID...
May 10, 2018: Sensors
https://www.readbyqxmd.com/read/29747668/magnitude-processing-of-symbolic-and-non-symbolic-proportions-an-fmri-study
#17
Julia Mock, Stefan Huber, Johannes Bloechle, Julia F Dietrich, Julia Bahnmueller, Johannes Rennig, Elise Klein, Korbinian Moeller
BACKGROUND: Recent research indicates that processing proportion magnitude is associated with activation in the intraparietal sulcus. Thus, brain areas associated with the processing of numbers (i.e., absolute magnitude) were activated during processing symbolic fractions as well as non-symbolic proportions. Here, we investigated systematically the cognitive processing of symbolic (e.g., fractions and decimals) and non-symbolic proportions (e.g., dot patterns and pie charts) in a two-stage procedure...
May 10, 2018: Behavioral and Brain Functions: BBF
https://www.readbyqxmd.com/read/29747539/illness-perceptions-association-and-prognosis-with-pain-and-physical-function-in-patients-with-non-cancer-musculoskeletal-pain-a-systematic-literature-review
#18
Edwin J de Raaij, Raymond W Ostelo, Francois Maissan, Jurgen Mollema, Harriët Wittink
Study Design Systematic review. Background In the literature, Illness Perceptions (IPs) have been reported to be important psychological factors associated with pain intensity and physical function in musculoskeletal pain (MP). Objective To assess the relationship of IPs with pain intensity and physical function in patients with MP. Methods Relevant databases including PubMed, Embase, PsycINFO, CINAHL and SPORTDiscus were systematically searched up to December 2017. Two authors (EdR, HW) independently performed the procedures according to the PRISMA and AMSTAR guidelines, including the Risk of Bias assessment, using the QUIPS tool...
May 10, 2018: Journal of Orthopaedic and Sports Physical Therapy
https://www.readbyqxmd.com/read/29747407/transcription-factors-as-therapeutic-targets-in-chronic-kidney-disease
#19
REVIEW
Akihito Hishikawa, Kaori Hayashi, Hiroshi Itoh
The growing number of patients with chronic kidney disease (CKD) is recognized as an emerging problem worldwide. Recent studies have indicated that deregulation of transcription factors is associated with the onset or progression of kidney disease. Several clinical trials indicated that regression of CKD may be feasible via activation of the transcription factor nuclear factor erythroid-2 related factor 2 (Nrf2), which suggests that transcription factors may be potential drug targets for CKD. Agents stabilizing hypoxia-inducible factor (HIF), which may be beneficial for renal anemia and renal protection, are also now under clinical trial...
May 9, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29739872/chemical-compound-based-direct-reprogramming-for-future-clinical-applications
#20
REVIEW
Yukimasa Takeda, Yoshinori Harada, Toshikazu Yoshikawa, Ping Dai
Recent studies have revealed that a combination of chemical compounds enables direct reprogramming from one somatic cell type into another without the use of transgenes by regulating cellular signaling pathways and epigenetic modifications. The generation of induced pluripotent stem (iPS) cells generally requires virus vector-mediated expression of multiple transcription factors, which might disrupt genomic integrity and proper cell functions. The direct reprogramming is a promising alternative to rapidly prepare different cell types by bypassing the pluripotent state...
June 29, 2018: Bioscience Reports
keyword
keyword
1717
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"