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Françoise Boudouresque, Carole Siret, Aurélie Dobric, Françoise Silvy, Philippe Soubeyran, Juan Iovanna, Dominique Lombardo, Yolande Berthois
The microRNA-200 (miR-200) family is frequently down-regulated in tumors, including pancreatic adenocarcinomas (PDACs). In this study we have examined the mechanisms involved in the loss of miR-200s in tumoral pancreatic cells. Whereas miR-200 gene promoters appear methylated in mature miR-200 deficient cell lines, miR-200 precursors are detected in nuclear but not cytoplasmic compartment of these cells, indicating that promoter hypermethylation is not sufficient to explain the deficit of mature miR-200s. The ribonuclease Monocyte Chemotactic Protein-induced Protein-1 (MCPiP1) may counteract Dicer1 in miRNA maturation process...
November 13, 2018: Oncotarget
Praveen K Suraneni, Seth J Corey, Michael J Hession, Rameez Ishaq, Arinola Awomolo, Shirin Hasan, Chirag Shah, Hui Liu, Amittha Wickrema, Najet Debili, John D Crispino, Elizabeth A Eklund, Yolande Chen
Megakaryocyte (MK) migration from the bone marrow periosteal niche toward the vascular niche is a prerequisite for proplatelet extension and release into the circulation. The mechanism for this highly coordinated process is poorly understood. Here we show that dynasore (DNSR), a small-molecule inhibitor of dynamins (DNMs), or short hairpin RNA knockdown of DNM2 and DNM3 impairs directional migration in a human MK cell line or MKs derived from cultured CD34+ cells. Because cell migration requires actin cytoskeletal rearrangements, we measured actin polymerization and the activity of cytoskeleton regulator RhoA and found them to be decreased after inhibition of DNM2 and DNM3...
December 11, 2018: Blood Advances
Anna Elisabeth Frick, Kristiaan Nackaerts, Johnny Moons, Yolande Lievens, Eric Verbeken, Maarten Lambrecht, Johan Coolen, Christophe Dooms, Johan Vansteenkiste, Paul De Leyn, Philippe Nafteux
OBJECTIVES: Combined modality treatment (CMT) for malignant pleural mesothelioma (MPM) remains a matter of debate regarding the choice of surgical procedure: extrapleural pneumonectomy (EPP) or pleurectomy/decortication. METHODS: We performed a prospective interventional cohort study between 2003 and 2014. All consecutive patients with any histological MPM subtype, ≤70 years old, World Health Organization performance status ≤1, medically fit for pneumonectomy and stage cT1-2cN0-2cM0 (TNM7) or lower were included...
December 6, 2018: European Journal of Cardio-thoracic Surgery
Simeng Zhou, Pierre Rousselot-Pailley, Lu Ren, Yolande Charmasson, Elise Courvoisier Dezord, Viviane Robert, Thierry Tron, Yasmina Mekmouche
Fungal laccases are robust multicopper oxidoreductases. Perfectly amenable to synthetic evolution, the fungal laccase scaffold is a potential generic for the production of tailored biocatalysts, which, in principle, can be secreted at substantial levels in industrially relevant organisms. In this chapter, the strategy we have developed for the rapid production of hundreds of milligram of laccase variants is detailed. It is based on the use of two heterologous expression hosts: the yeast Saccharomyces cerevisiae for a rapid upstream screening and the fungus Aspergillus niger for downstream production...
2018: Methods in Enzymology
Rodrigo Coutinho de Almeida, Yolande F M Ramos, Ahmed Mahfouz, Wouter den Hollander, Nico Lakenberg, Evelyn Houtman, Marcella van Hoolwerff, H Eka D Suchiman, Alejandro Rodríguez Ruiz, P Eline Slagboom, Hailiang Mei, Szymon M Kiełbasa, Rob G H H Nelissen, Marcel Reinders, Ingrid Meulenbelt
OBJECTIVE: To uncover the microRNA (miRNA) interactome of the osteoarthritis (OA) pathophysiological process in the cartilage. METHODS: We performed RNA sequencing in 130 samples (n=35 and n=30 pairs for messenger RNA (mRNA) and miRNA, respectively) on macroscopically preserved and lesioned OA cartilage from the same patient and performed differential expression (DE) analysis of miRNA and mRNAs. To build an OA-specific miRNA interactome, a prioritisation scheme was applied based on inverse Pearson's correlations and inverse DE of miRNAs and mRNAs...
December 1, 2018: Annals of the Rheumatic Diseases
Ruben W de Winter, Rob Adams, Giovanni Amoroso, Yolande Appelman, Lucia Ten Brinke, Bart Huybrechts, Pieternel van Exter, Robbert J de Winter
INTRODUCTION: The prevalence of the junctional ST-depression with tall symmetrical T-waves in a field triage system for ST-Elevation Myocardial Infarction (STEMI) is unknown. MATERIAL AND METHODS: We prospectively collected all transmitted 12-lead electrocardiograms (ECGs) from the STEMI field triage system in Amsterdam from 2011 to 2013. Electrocardiograms with junctional ST-depression with tall symmetrical T-waves were recognized and angiographic documentation and clinical follow up were collected...
October 18, 2018: Journal of Electrocardiology
Damien Leger, Yolande Esquirol, Claude Gronfier, Arnaud Metlaine
There are in France several millions of shift-workers and night-workers (20 to 25% of employees). These workers are therefore subject to variations in their working and rest schedules. These regular schedule changes are associated with repeated desynchronization of circadian biological clock. The negative impacts on sleep are insomnia, drowsiness, and reduced sleep time in 24hours. There is also a proven effect on the occurrence of a metabolic syndrome, with a likely effect on obesity, type 2 diabetes, blood pressure and coronary artery disease...
November 13, 2018: La Presse Médicale
Arnaud Metlaine, Damien Leger, Yolande Esquirol
Night work is not only a work performed at night. It has also to be considered as a specifically organized work with its own duties and moving tasks in a different relational context. Primary preventive assessments regarding work schedules and occupational associated constraints have to be considered beside family potential consequences. Taking account of the consensually accepted impact of night-shift work on health, particularly on sleep, we recommend an annual medical visit for night-shift workers. We also recommend to declare the night shift diseases to the Regional committee of occupational diseases via the "alinea 4"...
November 13, 2018: La Presse Médicale
Samantha Huo Yung Kai, Jean-Bernard Ruidavets, Camille Carles, Jean-Claude Marquie, Vanina Bongard, Damien Leger, Jean Ferrieres, Yolande Esquirol
BACKGROUND: The role of occupational stressors (OS) on blood pressure (BP) is often suspected, but asserting its impact remains uncertain. Our goal was to evaluate their impact on BP increase and on incident cases of hypertension over a 5-year period. METHODS: One thousand, one hundred and fifty-six men and women from the French prospective VISAT study were followed up over five-years (T1 to T2). Exposures to a large panel of OS (physical, organizational, psychosocial and employment categories) were collected...
November 16, 2018: Environmental Health: a Global Access Science Source
Francis N Saridin, Sigfried N T M Schouws, Jeroen de Jong, Yolande A L Pijnenburg, Annemiek Dols
C9Orf72 hexanucelotide repeat expansions have been associated with ALS and FTD. Although various psychiatric symptoms have been described in patients with C9Orf72 hexanucleotide repeat expansions, the full clinical spectrum has not yet been elucidated. There is increasing evidence that inflammation plays a role in the disease progression of ALS and FTD (1) and immune dysfunction is strongly associated with bipolar disorder. We present a case in which we hypothesize that inflammation may trigger a secondary mania as an expression of latent FTD/ALS in an older male C9orf72 hexanucleotide repeat expansion carrier...
November 14, 2018: Bipolar Disorders
Yolande Grobler, Chi Y Yun, David J Kahler, Casey M Bergman, Hangnoh Lee, Brian Oliver, Ruth Lehmann
Wolbachia is an intracellular bacterium that infects a remarkable range of insect hosts. Insects such as mosquitos act as vectors for many devastating human viruses such as Dengue, West Nile, and Zika. Remarkably, Wolbachia infection provides insect hosts with resistance to many arboviruses thereby rendering the insects ineffective as vectors. To utilize Wolbachia effectively as a tool against vector-borne viruses a better understanding of the host-Wolbachia relationship is needed. To investigate Wolbachia-insect interactions we used the Wolbachia/Drosophila model that provides a genetically tractable system for studying host-pathogen interactions...
November 2018: PLoS Pathogens
Andrea Fulgione, Martina Cimafonte, Bartolomeo Della Ventura, Marco Iannaccone, Concetta Ambrosino, Federico Capuano, Yolande Thérèse Rose Proroga, Raffaele Velotta, Rosanna Capparelli
Salmonella Typhimurium is one of the main causes of outbreaks and sporadic cases of human gastroenteritis. At present, the rapid detection of this pathogen is a major goal of biosensing technology applied to food safety. In fact, ISO standardized culture method takes up to ten days to provide a reliable response. In this paper, we describe a relatively simple protocol for detecting Salmonella Typhimurium in chicken meat based on a Quartz-Crystal Microbalance (QCM), which leads to a limit of detection (LOD) less than of 10° CFU/mL and requires a pre-enrichment step lasting only 2 h at 37 °C...
October 31, 2018: Scientific Reports
Erin J Howden, Ashley Bigaran, Rhys Beaudry, Steve Fraser, Steve Selig, Steve Foulkes, Yoland Antill, Sophie Nightingale, Sherene Loi, Mark J Haykowsky, André La Gerche
BACKGROUND: Anthracycline chemotherapy may be associated with decreased cardiac function and functional capacity measured as the peak oxygen uptake during exercise ([Formula: see text] peak). We sought to determine (a) whether a structured exercise training program would attenuate reductions in [Formula: see text] peak and (b) whether exercise cardiac imaging is a more sensitive marker of cardiac injury than the current standard of care resting left ventricular ejection fraction (LVEF)...
October 30, 2018: European Journal of Preventive Cardiology
Jorrit S Lemkes, Gladys N Janssens, Nina W van der Hoeven, Peter M van de Ven, Koen M J Marques, Alexander Nap, Maarten A H van Leeuwen, Yolande E A Appelman, Paul Knaapen, Niels J W Verouden, Cornelis P Allaart, Stijn L Brinckman, Colette E Saraber, Koos J Plomp, Jorik R Timmer, Elvin Kedhi, Renicus S Hermanides, Martijn Meuwissen, Jeroen Schaap, Arno P van der Weerdt, Albert C van Rossum, Robin Nijveldt, Niels van Royen
Aims: Patients with acute coronary syndrome who present initially with ST-elevation on the electrocardiogram but, subsequently, show complete normalization of the ST-segment and relief of symptoms before reperfusion therapy are referred to as transient ST-segment elevation myocardial infarction (STEMI) and pose a therapeutic challenge. It is unclear what the optimal timing of revascularization is for these patients and whether they should be treated with a STEMI-like or a non-ST-segment elevation myocardial infarction (NSTEMI)-like invasive approach...
October 26, 2018: European Heart Journal
Alexander Teumer, Layal Chaker, Stefan Groeneweg, Yong Li, Celia Di Munno, Caterina Barbieri, Ulla T Schultheiss, Michela Traglia, Tarunveer S Ahluwalia, Masato Akiyama, Emil Vincent R Appel, Dan E Arking, Alice Arnold, Arne Astrup, Marian Beekman, John P Beilby, Sofie Bekaert, Eric Boerwinkle, Suzanne J Brown, Marc De Buyzere, Purdey J Campbell, Graziano Ceresini, Charlotte Cerqueira, Francesco Cucca, Ian J Deary, Joris Deelen, Kai-Uwe Eckardt, Arif B Ekici, Johan G Eriksson, Luigi Ferrrucci, Tom Fiers, Edoardo Fiorillo, Ian Ford, Caroline S Fox, Christian Fuchsberger, Tessel E Galesloot, Christian Gieger, Martin Gögele, Alessandro De Grandi, Niels Grarup, Karin Halina Greiser, Kadri Haljas, Torben Hansen, Sarah E Harris, Diana van Heemst, Martin den Heijer, Andrew A Hicks, Wouter den Hollander, Georg Homuth, Jennie Hui, M Arfan Ikram, Till Ittermann, Richard A Jensen, Jiaojiao Jing, J Wouter Jukema, Eero Kajantie, Yoichiro Kamatani, Elisa Kasbohm, Jean-Marc Kaufman, Lambertus A Kiemeney, Margreet Kloppenburg, Florian Kronenberg, Michiaki Kubo, Jari Lahti, Bruno Lapauw, Shuo Li, David C M Liewald, Ee Mun Lim, Allan Linneberg, Michela Marina, Deborah Mascalzoni, Koichi Matsuda, Daniel Medenwald, Christa Meisinger, Ingrid Meulenbelt, Tim De Meyer, Henriette E Meyer Zu Schwabedissen, Rafael Mikolajczyk, Matthijs Moed, Romana T Netea-Maier, Ilja M Nolte, Yukinori Okada, Mauro Pala, Cristian Pattaro, Oluf Pedersen, Astrid Petersmann, Eleonora Porcu, Iris Postmus, Peter P Pramstaller, Bruce M Psaty, Yolande F M Ramos, Rajesh Rawal, Paul Redmond, J Brent Richards, Ernst R Rietzschel, Fernando Rivadeneira, Greet Roef, Jerome I Rotter, Cinzia F Sala, David Schlessinger, Elizabeth Selvin, P Eline Slagboom, Nicole Soranzo, Thorkild I A Sørensen, Timothy D Spector, John M Starr, David J Stott, Youri Taes, Daniel Taliun, Toshiko Tanaka, Betina Thuesen, Daniel Tiller, Daniela Toniolo, Andre G Uitterlinden, W Edward Visser, John P Walsh, Scott G Wilson, Bruce H R Wolffenbuttel, Qiong Yang, Hou-Feng Zheng, Anne Cappola, Robin P Peeters, Silvia Naitza, Henry Völzke, Serena Sanna, Anna Köttgen, Theo J Visser, Marco Medici
Thyroid dysfunction is an important public health problem, which affects 10% of the general population and increases the risk of cardiovascular morbidity and mortality. Many aspects of thyroid hormone regulation have only partly been elucidated, including its transport, metabolism, and genetic determinants. Here we report a large meta-analysis of genome-wide association studies for thyroid function and dysfunction, testing 8 million genetic variants in up to 72,167 individuals. One-hundred-and-nine independent genetic variants are associated with these traits...
October 26, 2018: Nature Communications
Yoland Savriama
This paper provides a step-by-step guide for the morphological analysis of corolla and the decomposition of corolla shape variation into its symmetric and asymmetric components. The shape and symmetric organisation of corolla are key traits in the developmental and evolutionary biology of flowering plants. The various spatial layout of petals can exhibit bilateral symmetry, rotational symmetry or more complex combination of symmetry types. Here, I describe a general landmark-based geometric morphometric framework for the full statistical shape analysis of corolla and exemplify its use with four fully worked out case studies including tissue treatment, imaging, landmark data collection, file formatting, and statistical analyses: (i) bilateral symmetry ( Fedia graciliflora ), (ii) two perpendicular axes of bilateral symmetry ( Erysimum mediohispanicum ), (iii) rotational symmetry ( Vinca minor ), and (iv) combined bilateral and rotational symmetry ( Trillium undulatum )...
2018: Frontiers in Plant Science
Flora T Gossink, Everard Vijverberg, Welmoed Krudop, Philip Scheltens, Max L Stek, Yolande A L Pijnenburg, Annemiek Dols
ABSTRACTA late onset frontal lobe syndrome (LOF) refers to a clinical syndrome with apathy, disinhibition, or stereotypical behavior arising in middle or late adulthood. Diagnostics are challenging, and both clinicians and patients need reliable predictors of progression to improve clinical guidance. In this longitudinal multicenter and genetically screened prospective study, 137 LOF patients with frontal behavior (FBI score≥11) and/or stereotypical behavior (SRI≥10) were included. Progression was defined as institutionalization, death, or progression of frontal or temporal atrophy at magnetic resonance imaging (MRI) after two years of follow up...
October 26, 2018: International Psychogeriatrics
Audrey Segura, Marine Bertoni, Pauline Auffret, Christophe Klopp, Olivier Bouchez, Clémence Genthon, Alexandra Durand, Yolande Bertin, Evelyne Forano
BACKGROUND: The cattle gastrointestinal tract (GIT) is the main enterohemorrhagic Escherichia coli (EHEC) reservoir. In order to identify nutrients required for the survival or multiplication of EHEC in the bovine GIT, we compared the transcriptomes of the EHEC O157:H7 reference strain EDL933 cultured in vitro in bovine digestive contents (DCs) (rumen, small intestine and rectum) using RNA-sequencing. RESULTS: Gene expression profiles showed that EHEC EDL933 activated common but also specific metabolic pathways to survive in the different bovine DCs...
October 23, 2018: BMC Genomics
Gianna Palmieri, Rosarita Tatè, Marta Gogliettino, Marco Balestrieri, Ilaria Rea, Monica Terracciano, Yolande Therese Proroga, Federico Capuano, Aniello Anastasio, Luca De Stefano
Synthetic antibacterial peptides are advanced weapons that scientists design and produce to confront current threats of harmful and mortal pathogens, which could affect humans in everyday life. Recently, many small amino acid sequences, greatly efficient in their antibacterial action, have been reported in the literature. To date, only a few synthetic peptides, acting at micromolar or even tenths of micromolar concentrations, are on the market as commercial products, mainly because of their high cost of production...
October 30, 2018: Bioconjugate Chemistry
Marta Del Campo, Daniela Galimberti, Naura Elias, Lynn Boonkamp, Yolande A Pijnenburg, John C van Swieten, Kelly Watts, Silvia Paciotti, Tommaso Beccari, William Hu, Charlotte E Teunissen
Objective: Frontotemporal lobar degeneration (FTLD) is the second most prevalent dementia in young patients and is characterized by the presence of two main protein aggregates in the brain, tau (FTLD-Tau) or TDP43 (FTLD-TDP), which likely require distinct pharmacological therapy. However, specific diagnosis of FTLD and its subtypes remains challenging due to largely overlapping clinical phenotypes. Here, we aimed to assess the clinical performance of novel cerebrospinal fluid (CSF) biomarkers for discrimination of FTLD and its pathological subtypes...
October 2018: Annals of Clinical and Translational Neurology
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