Dora Buzas, Huan Sun, Christine Toelzer, Sathish K N Yadav, Ufuk Borucu, Gunjan Gautam, Kapil Gupta, Joshua C Bufton, Julien Capin, Richard B Sessions, Frederic Garzoni, Imre Berger, Christiane Schaffitzel
Adenovirus-derived nanoparticles (ADDomer) comprise 60 copies of adenovirus penton base protein (PBP). ADDomer is thermostable, rendering the storage, transport, and deployment of ADDomer-based therapeutics independent of a cold chain. To expand the scope of ADDomers for new applications, we engineered ADDobodies, representing PBP crown domain, genetically separated from PBP multimerization domain. We inserted heterologous sequences into hyper-variable loops, resulting in monomeric, thermostable ADDobodies expressed at high yields in Escherichia coli...
January 1, 2024: Structure