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Hilde M Norum, Annika E Michelsen, Tove Lekva, Satish Arora, Kari Otterdal, Maria Belland Olsen, Xiang Yi Kong, Einar Gude, Arne K Andreassen, Dag Solbu, Kristjan Karason, Göran Dellgren, Lars Gullestad, Pål Aukrust, Thor Ueland
Cardiac allograft vasculopathy (CAV) causes heart failure after heart transplantation (HTx), but its pathogenesis is incompletely understood. Notch signaling, possibly modulated by everolimus (EVR), is essential for processes involved in CAV. We hypothesized that circulating Notch ligands would be dysregulated after HTx. We studied circulating delta-like Notch ligand 1 (DLL1) and periostin (POSTN) and CAV in de novo HTx recipients (n=70) randomized to standard or EVR-based, calcineurin inhibitor-free immunosuppression and in maintenance HTx recipients(n=41)...
October 12, 2018: American Journal of Transplantation
Zachary D Fox, Guihua Jiang, Kenneth K Y Ho, Kendal A Walker, Allen P Liu, Shaun M Kunisaki
BACKGROUND: The purpose of this study was to employ a novel ex vivo lung model of congenital diaphragmatic hernia (CDH) to determine how a mechanical compression affects early pulmonary development. METHODS: Day-15 whole fetal rat lungs (n = 6-12/group) from nitrofen-exposed and normal (vehicle only) dams were explanted and cultured ex vivo in compression microdevices (0.2 or 0.4 kPa) for 16 h to mimic physiologic compression forces that occur in CDH in vivo...
November 2018: Journal of Surgical Research
Tsuguhisa Nakayama, Naoki Sugimoto, Naoko Okada, Tadao Tsurumoto, Ryoto Mitsuyoshi, Shinya Takaishi, Daiya Asaka, Hiromi Kojima, Mamoru Yoshikawa, Yasuhiro Tanaka, Shin-Ichi Haruna
OBJECTIVE: Recently, JESREC score and mucosal eosinophil count have been used to diagnose eosinophilic chronic rhinosinusitis (ECRS) in Japan. However, it remains unknown whether the subtypes of CRS diagnosed by these criteria have different endotypes. In the present study, we investigated whether JESREC score and mucosal eosinophil count were appropriate for classification of CRS subgroups into endotypes. METHODS: A cross-sectional study involving 71 consecutive patients with CRS with nasal polyps (CRSwNP) and 13 control patients was performed...
September 19, 2018: Auris, Nasus, Larynx
Yoshiki Nakashima, Saifun Nahar, Chika Miyagi-Shiohira, Takao Kinjo, Zensei Toyoda, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Jiro Fujita, Hirofumi Noguchi
Liquid chromatography using a tandem mass spectrometer (LC-MS/MS) is a method of proteomic analysis. A shotgun analysis by LC-MS/MS comprehensively identifies proteins from tissues and cells with high resolution. The hepatic function of mice with acute hepatitis following the intraperitoneal administration of CCL4 was improved by the tail vein administration of the culture conditional medium (CM) of human mesenchymal stem cells from adipose tissue (hMSC-AT). In this study, a secreted protein expression analysis of hMSC-AT was performed using LC-MS/MS; 128 proteins were identified...
September 18, 2018: Cell Transplantation
Denise E Allard, Yan Wang, Jian Joel Li, Bridget Conley, Erin W Xu, David Sailer, Caellaigh Kimpston, Rebecca Notini, Collin-Jamal Smith, Emel Koseoglu, Joshua Starmer, Xiaopei L Zeng, James F Howard, Ahmet Hoke, Steven S Scherer, Maureen A Su
Chronic inflammatory demyelinating polyneuropathy (CIDP) and Guillain-Barre syndrome (GBS) are inflammatory neuropathies that affect humans and are characterized by peripheral nerve myelin destruction and macrophage-containing immune infiltrates. In contrast to the traditional view that the peripheral nerve is simply the target of autoimmunity, we report here that peripheral nerve Schwann cells exacerbate the autoimmune process through extracellular matrix (ECM) protein induction. In a spontaneous autoimmune peripheral polyneuropathy (SAPP) mouse model of inflammatory neuropathy and CIDP nerve biopsies, the ECM protein periostin (POSTN) was upregulated in affected sciatic nerves and was primarily expressed by Schwann cells...
October 1, 2018: Journal of Clinical Investigation
Francois Potus, Charles Colin Thomas Hindmarch, Kimberly J Dunham-Snary, Jeff Stafford, Stephen L Archer
Right ventricular failure (RVF) remains the leading cause of death in pulmonary arterial hypertension (PAH). We investigated the transcriptomic signature of RVF in hemodynamically well-phenotyped monocrotaline (MCT)-treated, male, Sprague-Dawley rats with severe PAH and decompensated RVF (increased right ventricular (RV) end diastolic volume (EDV), decreased cardiac output (CO), tricuspid annular plane systolic excursion (TAPSE) and ventricular-arterial decoupling). RNA sequencing revealed 2547 differentially regulated transcripts in MCT-RVF RVs...
September 12, 2018: International Journal of Molecular Sciences
Yasutaka Mitamura, Mika Murai, Chikage Mitoma, Masutaka Furue
Systemic fibrosing or sclerotic disorders are life-threatening, but only very limited treatment modalities are available for them. In recent years, periostin (POSTN), a major extracellular matrix component, was established by several studies as a novel key player in the progression of systemic fibrotic disease. In this research, we revealed the involvement of oxidative stress in the expression of POSTN induced by TGF- β 1 and IL-13 in dermal fibroblasts. We found that the antioxidant cinnamaldehyde activated the NRF2/HMOX1 pathway...
2018: Oxidative Medicine and Cellular Longevity
Carlo Cattrini, Alessandra Rubagotti, Pier Vitale Nuzzo, Linda Zinoli, Sandra Salvi, Simona Boccardo, Marta Perachino, Luigi Cerbone, Giacomo Vallome, Maria Maddalena Latocca, Elisa Zanardi, Francesco Boccardo
BACKGROUND: Overexpression of periostin (POSTN) is associated with prostate cancer (PCa) aggressiveness. We investigated the prognostic significance of POSTN expression in tumor biopsy samples of patients with PCa. METHODS: We scored POSTN expression by immunohistochemistry analysis on 215 PCa biopsy samples using an anti-POSTN-specific antibody. A total immunoreactive score (T-IRS) was calculated by adding the POSTN staining scores of stromal and epithelial tumor cells...
July 29, 2018: Clinical Genitourinary Cancer
Jung-Hyun Cho, Ah-Young Oh, Soyoung Park, So-Mi Kang, Min-Ho Yoon, Tae-Gyun Woo, Shin-Deok Hong, Jihwan Hwang, Nam-Chul Ha, Ho-Young Lee, Bum-Joon Park
Neurofibromatosis type 2 (NF2) syndrome is a very rare human genetic disease, and there has been no proper treatment for it until now. In our recent study, it has been reported that the loss of NF2 activates MAPK signaling through reduction of RKIP in a mesothelioma model. Here, we show that loss of NF2 induces reduction of the TGFβ receptor 2 (TβR2) expression, and an overwhelming expression of TGFβ receptor 1 (TβR1) is activated by physical stimuli such as pressure or heavy materials. Activated TβR1 induces the phosphorylation and degradation of RKIP...
August 22, 2018: Molecular Cancer Therapeutics
Jianquan Zhao, Tiewei Lv, Junjun Quan, Weian Zhao, Jing Song, Zhuolin Li, Han Lei, Wei Huang, Longke Ran
BACKGROUND: Identify genes probably associated with chronic heart failure and predict potential target genes for dilated cardiomyopathy using bioinformatics analyses. METHODS: Gene expression profiles (series number GSE3585 and GSE42955) of cardiomyopathy patients and healthy controls were downloaded from the Expression Omnibus Gene (GEO) database. Differential expression of genes (DEGS) between the two groups of total 14 cardiomyopathy patients and 10 healthy controls were subsequently identified by limma package of R...
August 16, 2018: Journal of Biomedical Science
Saffron A G Willis-Owen, Anna Thompson, Paul R Kemp, Michael I Polkey, William O C M Cookson, Miriam F Moffatt, Samantha A Natanek
Skeletal muscle dysfunction is a frequent extra-pulmonary manifestation of Chronic Obstructive Pulmonary Disease (COPD) with implications for both quality of life and survival. The underlying biology nevertheless remains poorly understood. We measured global gene transcription in the quadriceps using Affymetrix HuGene1.1ST arrays in an unselected cohort of 79 stable COPD patients in secondary care and 16 healthy age- and gender-matched controls. We detected 1,826 transcripts showing COPD-related variation. Eighteen exhibited ≥2fold changes (SLC22A3, FAM184B, CDKN1A, FST, LINC01405, MUSK, PANX1, ANKRD1, C12orf75, MYH1, POSTN, FRZB, TNC, ACTC1, LINC00310, MYH3, MYBPH and AREG)...
August 15, 2018: Scientific Reports
Masamitsu Hara, Kazuya Yokota, Takeyuki Saito, Kazu Kobayakawa, Ken Kijima, Shingo Yoshizaki, Ken Okazaki, Shigeo Yoshida, Yoshihiro Matsumoto, Katsumi Harimaya, Yasuharu Nakashima, Seiji Okada
BACKGROUND: Skeletal muscle injury (SMI) can cause physical disability due to insufficient recovery of the muscle. The development of muscle fibrosis after SMI has been widely regarded as a principal cause of this failure to recover. Periostin (Postn) exacerbates tissue fibrosis in various organs. We investigated whether Postn is involved in the pathophysiology after SMI. METHODS: Partial laceration injuries of the gastrocnemius were created in wild-type (WT) and Postn knockout (Postn) mice...
August 15, 2018: Journal of Bone and Joint Surgery. American Volume
Takahiro Ninomiya, Emiko Noguchi, Takenori Haruna, Masayo Hasegawa, Takuto Yoshida, Yukiko Yamashita, Mitsuhiro Okano, Naohiro Yoshida, Shinichi Haruna, Yasunori Sakuma, Shoichiro Ohta, Junya Ono, Kenji Izuhara, Masafumi Okada, Masanori Kidoguchi, Takahiro Tokunaga, Masayuki Okamoto, Masafumi Kanno, Masafumi Sakashita, Tetsuji Takabayashi, Norihiko Narita, Shigeharu Fujieda
We previously reported that chronic rhinosinusitis with nasal polyps (CRSwNP) was subdivided into four chronic rhinosinusitis (CRS) subtypes using the JESREC scoring system. We sought to identify the gene expression profile and biomarkers related with CRSwNP by RNA-sequence. RNA-sequencing was performed to identify differentially expressed genes between nasal polyps (NPs) and inferior turbinate mucosa from 6 patients with CRSwNP, and subsequently, quantitative real-time PCR was performed to verify the results...
July 30, 2018: Scientific Reports
Pascal O Zinn, Sanjay K Singh, Aikaterini Kotrotsou, Islam Hassan, Ginu Thomas, Markus M Luedi, Ahmed Elakkad, Nabil Elshafeey, Tagwa Idris, Jennifer Mosley, Joy Gumin, Gregory N Fuller, John Frederick deGroot, Veerabhadran Baladandayuthapani, Erik P Sulman, Ashok J Kumar, Raymond Sawaya, Frederick F Lang, David Piwnica-Worms, Rivka R Colen
PURPOSE: Radiomics is the extraction of multidimensional imaging-features which when correlated with genomics is termed radiogenomics. However, radiogenomic biological validation is not sufficiently described in the literature. We seek to establish causality between differential gene expression status and MRI-extracted radiomic-features in glioblastoma. METHODS: Radiogenomic predictions and validation were done using the Cancer Genome Atlas and Repository of Molecular Brain Neoplasia Data glioblastoma patients (N=93) and orthotopic xenografts (OX)(N=40)...
July 27, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Laura González-González, Javier Alonso
Tumor microenvironment is considered nowadays as one of the main players in cancer development and progression. Tumor microenvironment is highly complex and consists of non-tumor cells (i.e., cancer-associated fibroblast, endothelial cells, or infiltrating leukocytes) and a large list of extracellular matrix proteins and soluble factors. The way that microenvironment components interact among them and with the tumor cells is very complex and only partially understood. However, it is now clear that these interactions govern and modulate many of the cancer hallmarks such as cell proliferation, the resistance to death, the differentiation state of tumor cells, their ability to migrate and metastasize, and the immune response against tumor cells...
2018: Frontiers in Oncology
Dong Dong, Lufang Zhang, Li Jia, Wei Ji, Zhiyong Wang, Li Ren, Ruifang Niu, Yunli Zhou
BACKGROUND: Periostin (POSTN) plays an important role in numerous cancers, especially in gastrointestinal malignancy. The objective of this study was to investigate the diagnostic and prognostic role of serum POSTN in colorectal cancer (CRC). METHODS: Serum periostin, together with CEA, CA19.9, CA72.4, and CA242 levels were measured in samples from 108 patients with CRC and 56 healthy controls, and their correlation with clinical characteristics was further analyzed...
June 1, 2018: Clinical Laboratory
Dong Dong, Li Jia, Lufang Zhang, Na Ma, Aimin Zhang, Yunli Zhou, Li Ren
Pancreatic ductal adenocarcinoma (PDAC) is a highly malignant tumor with few biomarkers to guide treatment options. Carbohydrate antigen 19.9 (CA19.9), the most frequently used biomarker for PDAC, is not sensitive and specific enough for the detection of the disease. This study aimed to evaluate serum periostin (POSTN) and CA242 as potential diagnostic biomarkers complementing CA19.9 in detecting pancreatic cancer. Blood samples were from 362 participants, including 213 patients with different stages of PDAC, 75 patients with benign pancreatic disease, and 74 healthy individuals...
September 2018: Cancer Science
Yu-Ching Wei, Sheau-Fang Yang, Shih-Lun Chang, Tzu-Ju Chen, Sung-Wei Lee, Hung-Sung Chen, Li-Ching Lin, Chien-Feng Li
Purpose: Nasopharyngeal carcinoma (NPC) is a heterogeneous disease. We searched for genes that function in cell adhesion in GSE12452, a published transcriptomic database. We found that POSTN, which encodes periostin (POSTN), was significantly upregulated in NPC tumorigenesis. Herein, we sought to analyze the expression of POSTN and its prognostic significances in patients with NPC. Materials and methods: In this single-institution retrospective study, we determined and analyzed POSTN expression by immunohistochemistry and H-score method, respectively, in 124 patients with NPC...
2018: OncoTargets and Therapy
Fumiko Nakazeki, Masataka Nishiga, Takahiro Horie, Hitoo Nishi, Yasuhiro Nakashima, Osamu Baba, Yasuhide Kuwabara, Tomohiro Nishino, Tetsushi Nakao, Yuya Ide, Satoshi Koyama, Masahiro Kimura, Shuhei Tsuji, Naoya Sowa, Shigeo Yoshida, Simon J Conway, Motoko Yanagita, Takeshi Kimura, Koh Ono
Recent evidence suggests that the accumulation of macrophages as a result of obesity-induced adipose tissue hypoxia is crucial for the regulation of tissue fibrosis, but the molecular mechanisms underlying adipose tissue fibrosis are still unknown. In this study, we revealed that periostin (Postn) is produced at extraordinary levels by adipose tissue after feeding with a high-fat diet (HFD). Postn was secreted at least from macrophages in visceral adipose tissue during the development of obesity, possibly due to hypoxia...
June 4, 2018: Scientific Reports
Jiehong Zeng, Zhengkai Liu, Shuwen Sun, Jianhong Xie, Li Cao, Pin Lv, Shengdan Nie, Bao Zhang, Bowen Xie, Siyuan Peng, Bo Jiang
Periostin (POSTN) secreted by intrahepatic cholangiocarcinoma stem cells (ICSCs) serves important roles in promoting tumor progression. The present study aimed to investigate POSTN-recruited tumor-associated macrophages (TAMs) in intrahepatic cholangiocarcinoma (ICC). A total of 50 cases were used to investigate the distribution of ICSCs and TAMs in ICC. HCCC-9810 cells were sorted by cluster of differentiation (CD)44, the expression of POSTN of CD44+ (cancer stem cells) and CD44- cells (non-cancer stem cells), and medium were evaluated by western blot analysis...
June 2018: Oncology Letters
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