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Lymphatic endothelial cell

Vivek Kasinath, Osman A Yilmam, Mayuko Uehara, Liwei Jiang, Farideh Ordikhani, Xiaofei Li, David J Salant, Reza Abdi
Crescentic glomerulonephritis is an inflammatory condition characterized by rapid deterioration of kidney function. Previous studies of crescentic glomerulonephritis have focused on immune activation in the kidney. However, the role of fibroblastic reticular cells, which reside in the stromal compartment of the kidney lymph node, has not been studied in this condition. We investigated the activation of kidney lymph node-resident fibroblastic reticular cells in nephrotoxic serum nephritis, a classic murine model of crescentic glomerulonephritis...
November 29, 2018: Kidney International
Florian Winkler, Katia Herz, Sarah Rieck, Kenichi Kimura, Tianyuan Hu, Wilhelm Röll, Michael Hesse, Bernd K Fleischmann, Daniela Wenzel
For the monitoring of vascular growth as well as adaptive or therapeutic (re)vascularization endothelial-specific reporter mouse models are valuable tools. However, currently available mouse models have limitations, because not all endothelial cells express the reporter in all developmental stages. We have generated PECAM/eGFP embryonic stem (ES) cell and mouse lines where the reporter gene labels PECAM+ endothelial cells and vessels with high specificity. Native eGFP expression and PECAM staining were highly co-localized in vessels of various organs at embryonic stages E9...
December 4, 2018: Scientific Reports
Kaya Xu, Jea-Young Lee, Yuji Kaneko, Julian P Tuazon, Fernando Vale, Harry van Loveren, Cesario V Borlongan
Despite mounting evidence of a massive peripheral inflammatory response accompanying stroke, the ability of intracerebrally transplanted cells to migrate to the periphery and sequester systemic inflammation remains unexamined. Here, we tested the hypothesis that human bone marrow mesenchymal stromal cells intracerebrally transplanted in the brain of adult rats subjected to experimental stroke can migrate to the spleen, a vital organ that confers peripheral inflammation after stroke. Adult Sprague-Dawley rats received sham or experimental stroke via the one-hour middle cerebral artery occlusion model...
December 4, 2018: Haematologica
Brad W Porter, Thara K Venkatappa
Latent tuberculosis infection (LTBI) is diagnosed immunologically using the Mantoux tuberculin skin test (TST) or interferon-gamma release assays (IGRAs). While widely used, immunodiagnostics can produce false negative or false positive results. Pathogen biomarkers provide an alternative, but direct detection in LTBI and extrapulmonary TB cases is challenging. Mycobacterium tuberculosis grows slowly, has limited hematogenous movement, is protected by a lipid rich cell wall, and produces low levels of secreted factors...
December 2018: Tuberculosis
Ssu-Han Wang, Gunn-Guang Liou, Szu-Heng Liu, Jeffrey S Chang, Jenn-Ren Hsiao, Yi-Chen Yen, Yu-Lin Chen, Wan-Ling Wu, Jang-Yang Chang, Ya-Wen Chen
Oral squamous cell carcinoma (OSCC) LN1-l cells previously showed greater capacities for lymphangiogenesis and lymph node metastasis compared to their parental OEC-M1 cells, in addition to an ability to enhance the migration and tube formation of lymphatic endothelial cells (LECs). Purified by a series of differential centrifugations and characterized using electron microscopy, dynamic light scattering, and western blot, LN1-1 cell-derived extracellular vesicles (LN1-1 EVs) were shown to promote LEC migration, tube formation, and uptake by LECs more effectively than did OEC-M1 cell-derived EVs (OEC-M1 EVs)...
November 28, 2018: International Journal of Cancer. Journal International du Cancer
Keiko Nonomura, Viktor Lukacs, Daniel T Sweet, Lauren M Goddard, Akemi Kanie, Tess Whitwam, Sanjeev S Ranade, Toshihiko Fujimori, Mark L Kahn, Ardem Patapoutian
PIEZO1 is a cation channel that is activated by mechanical forces such as fluid shear stress or membrane stretch. PIEZO1 loss-of-function mutations in patients are associated with congenital lymphedema with pleural effusion. However, the mechanistic link between PIEZO1 function and the development or function of the lymphatic system is currently unknown. Here, we analyzed two mouse lines lacking PIEZO1 in endothelial cells (via Tie2Cre or Lyve1Cre ) and found that they exhibited pleural effusion and died postnatally...
November 27, 2018: Proceedings of the National Academy of Sciences of the United States of America
Zhi Zheng, Yong-Zhi Zeng, Kun Ren, Xiao Zhu, Ying Tan, Yi Li, Qian Li, Guang-Hui Yi
Inflammation-induced lymphangiogenesis is a widely accepted concept. However, most of the inflammatory factors and their related mechanisms have not been clarified. It has been reported that sphingosine-1-phosphate (S1P) is not only closely related to the chronic inflammatory process but also affects angiogenesis. Therefore, we investigated the inflammatory effects of S1P on human lymphatic endothelial cells (HLECs). Our results showed that S1P promotes tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) secretion in HLECs...
November 23, 2018: International Immunopharmacology
Shinji Yamada, Mika K Kaneko, Yoshikazu Furusawa, Shunsuke Itai, Masato Sano, Takuro Nakamura, Miyuki Yanaka, Saori Handa, Kayo Hisamatsu, Yoshimi Nakamura, Masanori Koyanagi, Masato Fukui, Hiroyuki Harada, Yukinari Kato
Podoplanin (PDPN) is expressed in type I alveolar cells, kidney podocytes, and lymphatic endothelial cells. We have characterized the PDPNs of various animal species using specific anti-PDPN monoclonal antibodies (mAbs). In this study, we investigated whether these anti-PDPN mAbs cross-react with goat PDPN (gPDPN). Flow cytometry demonstrated that the anti-bovine PDPN mAb PMab-44 (IgG1 , kappa) reacts with gPDPN, which is overexpressed in CHO-K1 cells. Using immunohistochemical analysis, type I alveolar cells of goat lung were strongly detected by PMab-44...
November 20, 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Hiroaki Yokomori, Wataru Ando, Fumihiko Kaneko, Hidekazu Suzuki, Koji Igarashi, Masaya Oda
Vascular endothelial growth factor (VEGF) and autotaxin (ATX) play important roles in embryonic vasculogenesis and cancer progression. This study examines whether these two angiogenic factors cooperate in the mechanism that regulates vascular development during the progression of chronic viral hepatitis C (CVH-C) (Inuyama classification, F1-F4). First, surgical wedge biopsy specimens and needle biopsy specimens were obtained. Immunohistochemical staining for ATX and vascular endothelial growth factor receptor was assessed in serial sections...
December 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
Masakazu Ishii, Manami Takahashi, Juri Murakami, Takahiro Yanagisawa, Masahiro Nishimura
Vascular endothelial cell growth factor-C (VEGF-C) is a member of the VEGF family and plays a role in various biological activities. VEGF-C enhances proliferation and migration of lymphatic endothelial cells and vascular endothelial cells through VEGF receptor 2 (VEGFR2) and/or receptor 3 (VEGFR3), and thereby induces lymphangiogenesis or angiogenesis. However, it remains unclear whether VEGF-C promotes the migration of mesenchymal stem cells (MSCs). Here, we investigated the effects of VEGF-C on the migration of MSCs and evaluated the underlying molecular mechanisms...
November 15, 2018: Molecular and Cellular Biochemistry
Liping Su, Xiaocen Kong, Szeyun Lim, Szejie Loo, Shihua Tan, Kiankeong Poh, James Dutton, Colin Stewart, Stuart Cook, Xiaofei Su, Jianhua Ma, Jianyi Zhang, Lei Ye
BACKGROUND: We have shown that the differentiation of human-induced pluripotent stem cells (hiPSCs) into endothelial cells (ECs) is more efficient when performed with a 3-dimensional (3D) scaffold of biomaterial than in monolayers. The current study aims to further increase hiPSC-EC differentiation efficiency by deciphering the signaling pathways in 3D scaffolds. METHODS AND RESULTS: We modified our 3D protocol by using U-46619 to upregulate both p38 mitogen-activated protein kinase (p38MAPK) and extracellular signal-regulated kinase 1/2 (ERK1/2) signaling, which increased the differentiation efficiency (as measured by CD31 expression) to as high as 89% in two established hiPSC lines...
November 15, 2018: Stem Cell Research & Therapy
Mugurel Constantin Rusu, Sorin Hostiuc
The study of cardiac interstitial Cajal-like cells (ICLCs) began in 2005 and continued until 2010, when these cells were renamed as telocytes (TCs). Since then, numerous papers on cardiac ICLCs and TCs have been published. However, in the initial descriptions upon which further research was based, lymphatic endothelial cells (LECs) and initial lymphatics were not considered. No specific antibodies for LECs (such as podoplanin or LYVE-1) were used in cardiac TC studies, although ultrastructurally, LECs and TCs have similar morphological traits, including the lack of a basal lamina...
November 12, 2018: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
Tao Han, Jun Yan, Haini Chen, Yi Ji, Jianbing Chen, Jie Cui, Weimin Shen, Jijun Zou
Hypoxia‑inducible factor‑1α (HIF‑1α) is upregulated in various tumors and associated with lymphangiogenesis and angiogenesis during tumor development and metastasis. However, the role of HIF‑1α in cystic lymphatic malformations (cLM) remains unclear. In the present study, expression of HIF‑1α and vascular endothelial growth factor receptor 3 (VEGFR‑3) was evaluated in 20 pairs of cLM specimens from patients who accepted curative surgery at Children's Hospital of Nanjing Medical University (Nanjing, China)...
January 2019: International Journal of Oncology
Jacqui A McGovern, Abbas Shafiee, Ferdinand Wagner, Christoph A Lahr, Marietta Landgraf, Christoph Meinert, Elizabeth D Williams, Pamela J Russell, Judith A Clements, Daniela Loessner, Boris M Holzapfel, Gail P Risbridger, Dietmar W Hutmacher
The primary tumor microenvironment is inherently important in prostate cancer (PCa) initiation, growth and metastasis. However, most current PCa animal models are based on the injection of cancer cells into the blood circulation and bypass the first steps of the metastatic cascade, hence failing to investigate the influence of the primary tumor microenvironment on PCa metastasis. Here, we investigated the spontaneous metastasis of PC3 human PCa cells from humanized prostate tissue, containing cancer-associated fibroblasts (CAFs) and prostate lymphatic and blood vessel endothelial cells (BVEC), to humanized tissue-engineered bone constructs (hTEBC) in NOD-SCID IL2Rγnull (NSG) mice...
November 13, 2018: Cancers
Tatsuya Osaki, Jean C Serrano, Roger D Kamm
In this study, we modeled lymphangiogenesis and vascular angiogenesis in a microdevice using a tissue engineering approach. Lymphatic vessels (LV) and blood vessels (BV) were fabricated by sacrificial molding with seeding human lymphatic endothelial cells and human umbilical vein endothelial cells into molded microchannels (600 μm diameter). During subsequent perfusion culture, lymphangiogenesis and vascular angiogenesis were induced by addition of phorbol 12-myristate 13-acetate (PMA) and VEGF-C or VEGF-A characterized by podoplanin and Prox-1 expression...
September 2018: Regenerative Engineering and Translational Medicine
Seiji Shigetomi, Yorihisa Imanishi, Katsushi Shibata, Nobuya Sakai, Koji Sakamoto, Ryoichi Fujii, Noboru Habu, Kuninori Otsuka, Yoichiro Sato, Yoshihiro Watanabe, Masayuki Shimoda, Kaori Kameyama, Hiroyuki Ozawa, Toshiki Tomita, Kaoru Ogawa
Tumor cell-derived vascular endothelial growth factor (VEGF)-C has been primarily implicated in promoting lymphangiogenesis by activating Flt-4 (VEGFR-3) expressed on lymphatic endothelial cells via a paracrine mechanism. Flt4 has also been shown to be expressed selectively in subsets of cancer cells. However, little is known about the functional role of VEGF-C/Flt4 signaling via an autocrine mechanism, as well as the clinicopathological implication of the VEGF-C/Flt4 axis and its downstream effector molecules, in head and neck squamous cell carcinoma (HNSCC), including oral squamous cell carcinoma (OSCC)...
2018: American Journal of Cancer Research
Alessandra Capuano, Eliana Pivetta, Francesca Baldissera, Giulia Bosisio, Bruna Wassermann, Francesco Bucciotti, Alfonso Colombatti, Patrizia Sabatelli, Roberto Doliana, Paola Spessotto
Lymphatic vessels (LVs) play a pivotal role in the control of tissue homeostasis and also have emerged as important regulators of immunity, inflammation and tumor metastasis. EMILIN-1 is the first ECM protein identified as a structural modulator of the growth and maintenance of LV; accordingly, Emilin1-/- mice display lymphatic morphological alterations leading to functional defects as mild lymphedema, leakage and compromised lymph drainage. Many EMILIN-1 functions are exerted by the binding of its gC1q domain with the E933 residue of α4 and α9β1 integrins...
October 26, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
Satomi Arimoto, Takumi Hasegawa, Daisuke Takeda, Izumi Saito, Rika Amano, Masaya Akashi, Takahide Komori
BACKGROUND/AIM: Tumor lymphangiogenesis plays a key role in lymph node (LN) metastasis in oral squamous cell carcinoma (OSCC). The purpose of this study was to investigate podoplanin and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) and their relationship to nodal metastasis and other clinicopathological variables. PATIENTS AND METHODS: Podoplanin and LYVE-1 expression of the primary tumor and normal tissue were investigated by means of a quantitative real-time PCR assay and immunohistochemistry in samples from 33 cases of OSCC...
November 2018: Anticancer Research
Tristan Reuer, Ann-Charlott Schneider, Bertan Cakir, Anima D Bühler, Johanna M Walz, Thabo Lapp, Clemens Lange, Hansjürgen Agostini, Günther Schlunck, Claus Cursiefen, Thomas Reinhard, Felix Bock, Andreas Stahl
Purpose: Corneal vascularization significantly increases the risk for graft rejection after keratoplasty. Semaphorin 3F (Sema3F) is a known modulator of physiologic avascularity in the outer retina. The aim of this study was to investigate whether Sema3F is involved in maintaining corneal avascularity and can reduce the risk for corneal graft rejection. Methods: Corneal Sema3F expression was investigated using immunohistochemistry and qPCR in human and murine tissue...
October 1, 2018: Investigative Ophthalmology & Visual Science
Orawin Prangsaengtong, Phatcharida Jantaree, Kriengsak Lirdprapamongkol, Jisnuson Svasti, Keiichi Koizumi
Lymphangiogenesis, the formation of lymphatic vessels from preexisting ones, promotes cancer growth and metastasis. Finding natural compounds with anti-lymphangiogenic activity will be useful for preventive treatment of lymphatic metastasis. Shikonin, an ingredient of a traditional Japanese and Chinese medicinal herb Lithospermum erythrorhizon, has been widely used in several pharmaceutical and cosmetic preparations, as well as in food colorants. Shikonin has been reported to inhibit lymphangiogenesis in vitro, but the mechanism of inhibition has not been determined...
2018: Biological & Pharmaceutical Bulletin
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