Diede Houbaert, Apostolos Panagiotis Nikolakopoulos, Kathryn A Jacobs, Odeta Meçe, Jana Roels, Gautam Shankar, Madhur Agrawal, Sanket More, Maarten Ganne, Kristine Rillaerts, Louis Boon, Magdalena Swoboda, Max Nobis, Larissa Mourao, Francesca Bosisio, Niels Vandamme, Gabriele Bergers, Colinda L G J Scheele, Patrizia Agostinis
Lymphatic endothelial cells (LECs) of the lymph node (LN) parenchyma orchestrate leukocyte trafficking and peripheral T cell dynamics. T cell responses to immunotherapy largely rely on peripheral T cell recruitment in tumors. Yet, a systematic and molecular understanding of how LECs within the LNs control T cell dynamics under steady-state and tumor-bearing conditions is lacking. Intravital imaging combined with immune phenotyping shows that LEC-specific deletion of the essential autophagy gene Atg5 alters intranodal positioning of lymphocytes and accrues their persistence in the LNs by increasing the availability of the main egress signal sphingosine-1-phosphate...
March 28, 2024: Cell Reports