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Hsien-Kuo Chin, Chi-Ting Horng, Yi-Shan Liu, Chi-Cheng Lu, Chen-Ying Su, Pei-Syuan Chen, Hong-Yi Chiu, Fuu-Jen Tsai, Po-Chuen Shieh, Jai-Sing Yang
Anti-angiogenesis is one of the most general clinical obstacles in cancer chemotherapy. Kaempferol is a flavonoid phytochemical found in many fruits and vegetables. Our previous study revealed that kaempferol triggered apoptosis in human umbilical vein endothelial cells (HUVECs) by ROS‑mediated p53/ATM/death receptor signaling. However, the anti‑angiogenic potential of kaempferol remains unclear and its underlying mechanism warranted further exploration in VEGF‑stimulated HUVECs. In the present study, kaempferol significantly reduced VEGF‑stimulated HUVEC viability...
May 2018: Oncology Reports
Dunsfort N Malejane, Peter Tinyani, Puffy Soundy, Yasmina Sultanbawa, Dharini Sivakumar
This study investigated the effect of deficit irrigation at three management allowable depletion levels (MAD) 25%, 50%, and 75% on phytochemicals, ascorbic acid, antioxidant activity, glucose, fructose, and fresh and dry mass in two green leafy lettuce cultivars (Lollo Bionda and Vera) at harvest. Genotype response was observed during deficit irrigation on fresh and dry mass at harvest. Vera revealed similar fresh mass at MAD levels 25% and 50%. Chicoric acid, caftaric acid, and chlorogenic and caffeic acid tended to increase at 50% MAD, while kaempferol, quercetin, and myricetin increased at 75% MAD in both cultivars indicating increasing antioxidant properties...
March 2018: Food Science & Nutrition
Elke von Son-de Fernex, Ángel Alonso-Díaz Miguel, Braulio Valles-de la Mora, Pedro Mendoza-de Gives, Epigmenio Castillo-Gallegos, Alejandro Zamilpa, Manases González-Cortazar
The aims of this study were: 1) to assess the anthelmintic effect of Gliricidia sepium on the establishment of C. punctata third-stage larvae (L3 ) in calves, and 2) to isolate and to elucidate an anti-exsheathment phytochemical from the plant offered during the trial. Twelve ¾ Holstein × Zebu calves were divided in two experimental groups: control (T1) and treatment (T2) (n = 6). After adaptation, each calf was infected with an oral dose of 400 C. punctata L3 /Kg LW. Basal diet consisted of Digitaria decumbens hay (6...
March 15, 2018: Veterinary Parasitology
Xiu-Mei Li, Xue-Gang Luo, Jun-Fang He, Nan Wang, Hao Zhou, Pei-Long Yang, Tong-Cun Zhang
Hypericum ascyron L. (Great St. Johnswort), which belongs to the Hypericaceae family, has been used for the treatment of hematemesis, metrorrhagia, rheumatism, swelling, stomach ache, abscesses, dysentery and irregular menstruation for >2,000 years in China. The aim of the present study was to clarify the anticancer activity compounds from H. ascyron L. and the underlying molecular mechanism. Anticancer activity of H. ascyron L. extract was evaluated using an MTT assay. To confirm the anticancer mechanism of activity compounds, Hoechst 33258, Annexin V-fluorescein isothiocyanate/propidium iodide, 2',7'-dichlorodihydrofluorescein diacetate, rhodamine 123 staining and caspase-3 activity analysis were performed...
March 2018: Oncology Letters
Jia Yang, Peng Xiao, Jiaming Sun, Liang Guo
PURPOSE: Melanoma is an aggressive form of human cancer with limited treatment options currently available. The present study was aimed to evaluate the anticancer activity of kaempferol (KAM) against the human malignant melanoma A375 cell line along with evaluation of its effects on apoptosis, cell cycle, cell migration and m-TOR/PI3K/AKT pathway. METHODS: Effects on cell viability were assessed by MTT assay while clonogenic assay measured the effects of KAM on colony formation...
January 2018: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
Inês Amaral, Cláudia Silva, Ana Correia-Branco, Fátima Martel
This work aimed to investigate the effect of metformin on cellular glucose uptake and metabolism by breast cancer cells, as a mechanism contributing to its anticancer properties. Estrogen and progesterone receptor-positive (MCF-7) and triple-negative (MDA-MB-231) breast cancer cell lines were used as in vitro models of breast cancer. Short-term (26 min) exposure of MCF-7 and MDA-MB-231 cells to metformin inhibited uptake of3 H-deoxy-D-glucose (3 H-DG). In contrast, long-term (24 h) exposure to metformin (5 μM-1 mM) concentration-dependently increased3 H-DG uptake in both cell lines...
March 15, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
José Pinela, Lillian Barros, João C M Barreira, Ana Maria Carvalho, M Beatriz P P Oliveira, Celestino Santos-Buelga, Isabel C F R Ferreira
The effects of γ-ray irradiation and modified atmosphere packaging (MAP) on watercress (Nasturtium officinale R. Br.) phenolic compounds were evaluated after 7-day storage at 4 °C. Irradiation doses of 1, 2 and 5 kGy were tested, as well as vacuum-packaging and MAP enriched with 100% N2 and Ar. A non-irradiated, air-packaged control was included in all experiments. p-Coumaric acid was the most abundant compound in fresh watercress, followed by quercetin-3-O-sophoroside and isorhamnetin-O-hydroxyferuloylhexoside-O-hexoside...
July 15, 2018: Food Chemistry
Jânio S Santos, Carolina T P Deolindo, Jessica F Hoffmann, Fabio C Chaves, Leonardo do Prado-Silva, Anderson S Sant'Ana, Luciana Azevedo, Mariana Araújo Vieira do Carmo, Daniel Granato
A statistical optimization study was conducted to obtain a tea containing fermented rooibos (Aspalathus linearis), white tea (Camellia sinensis var. sinensis), and roasted mate (Ilex paraguariensis). An optimal combination of these species was proposed. This optimized tea inhibited 64% the lipoperoxidation in vitro and presented a high phenolic content, especially kaempferol, (+)-catechin, (-)-epicatechin, rutin, (-)-epigallocatechin, and (-)-epicatechin-2-O-gallate. Indeed, the antioxidant effect was confirmed by decreasing 30% the reactive oxygen species generation in human hepatoma carcinoma cells (HepG2, 100 and 240 µg/mL)...
July 15, 2018: Food Chemistry
María R Flores-Ocelotl, Nora H Rosas-Murrieta, Diego A Moreno, Verónica Vallejo-Ruiz, Julio Reyes-Leyva, Fabiola Domínguez, Gerardo Santos-López
BACKGROUND: Urtica dioica, Taraxacum officinale, Calea integrifolia and Caesalpinia pulcherrima are widely used all over the world for treatment of different illnesses. In Mexico, these plants are traditionally used to alleviate or counteract rheumatism and inflammatory muscle diseases. In the present study we evaluated the activity of aqueous and methanolic extracts of these four plants, on the replication of dengue virus serotype 2 (DENV2). METHODS: Extraction process was carried out in a Soxtherm® system at 60, 85 and 120 °C; a chemical fractionation in silica gel chromatography was performed and compounds present in the active fractions were identified by HPLC-DAD-ESI/MSn...
March 16, 2018: BMC Complementary and Alternative Medicine
Zhou Hong, Juan Li, Sangita Biswas, Cheng-Shuai Jiang, Yu Huang, Tao Sun, Yang Niu, Jian-Qiang Yu, Wei-Qi Li, Yao Yao
Tamarix ramosissima is a traditional Chinese herbal medicine used for rheumatoid arthritis (RA) treatment in Northwest China. Chemical investigation of EtOH/H2O extracts of T. ramosissima led to the discovery of a new flavonol, ramosissimin (1), together with the known flavonoids compounds quercetin (2), quercetin-3'4'-dimethylether (3) and kaempferol (4). By means of high resolution electrospray ionization mass spectroscopy (HRESIMS) and 1D and 2D-NMR experiments, and after comparison with literature data, the structures of the compounds were determined...
March 5, 2018: Die Pharmazie
Cheol-Jung Lee, Su-Jin Moon, Jeong-Hee Jeong, Sangbae Lee, Mee-Hyun Lee, Sun-Mi Yoo, Hye Suk Lee, Han Chang Kang, Joo Young Lee, Weon Sun Lee, Hee-Jin Lee, Eun-Kyung Kim, Joo-Yeon Jhun, Mi-La Cho, Jun-Ki Min, Yong-Yeon Cho
Rheumatoid arthritis (RA) is a systemic inflammatory disease that mainly affects the synovial joints. Although involvement of the fibroblast growth factor (FGF) signaling pathway has been suggested as an important modulator in RA development, no clear evidence has been provided. In this study, we found that synovial fluid basic FGF (bFGF) concentration was significantly higher in RA than in osteoarthritis (OA) patients. bFGF stimulates proliferation and migration of human fibroblast-like synoviocytes (FLSs) by activation of the bFGF-FGF receptor 3 (FGFR3)-ribosomal S6 kinase 2 (RSK2) signaling axis...
March 14, 2018: Cell Death & Disease
Dorota Woźniak, Sylwester Ślusarczyk, Krzysztof Domaradzki, Andrzej Dryś, Adam Matkowski
European Pharmacopoeia accepts two equivalent species Solidago canadensis L. and S. gigantea Ait. as Goldenrod (Solidaginis herba). We compared phytochemical profile of both species from invasive populations in Poland. Further, we compared in vitro antimutagenic and antioxidant activities of solvent extracts from aerial (AP) and underground parts (UP). In S. gigantea, flavonoid profile was dominated by quercetin glycosides, with quercitrin as the major compound. In S. canadensis, quercetin and kaempferol rutinosides were two major constituents...
March 14, 2018: Chemistry & Biodiversity
Rebai Ben Ammar, Tomofumi Miyamoto, Leila Chekir-Ghedira, Kamel Ghedira, Marie-Aleth Lacaille-Dubois
From the butanolic and the ethyl acetate extracts of Rhamnus alaternus L root bark and leaves, three new anthraquinone glycosides, alaternosides A-C (1,4,6,8 tetrahydroxy-3 methyl anthraquinone 1-O-ß-D-glucopyranosyl-4,6-di-O-α-L-rhamnopyranoside (1); 1,2,6,8 tetrahydroxy-3 methyl anthraquinone 8-O-ß-D-glucopyranoside (2) and 1, 6 dihydroxy-3 methyl 6 [2'-Me (heptoxy)] anthraquinone (3)) were isolated and elucidated together with the two known anthraquinone glycosides, Physcion-8-O-rutinoside (4) and emodin-6-O-α-L-rhamnoside (5) as well as with the known kaempferol-7-methylether (6), β-sitosterol (7) and β-sitosterol-3-O-glycoside (8)...
March 13, 2018: Natural Product Research
Jinan Tuma Sabah, Razauden Mohamed Zulkifli, Shafinaz Shahir, Farediah Ahmed, Mohammed Rafiq Abdul Kadir, Zarita Zakaria
Distinctive bioactivities possessed by luteolin (3', 4', 5, 7-tetrahydroxy-flavone) are advantageous for sundry practical applications. This paper reports the in vitro selection and characterization of single stranded-DNA (ssDNA) aptamers, specific for luteolin (LUT). 76-mer library containing 1015 randomized ssDNA were screened via systematic evolution of ligands by exponential enrichment (SELEX). The recovered ssDNA pool from the 8th round was amplified with unlabeled primers and cloned into PSTBlue-1 vector prior to sequencing...
March 6, 2018: Analytical Biochemistry
Kai Feng, Zhi-Sheng Xu, Jie-Xia Liu, Jing-Wen Li, Feng Wang, Ai-Sheng Xiong
This study showed that a galactosyltransferase, AgUCGalT1, is involved in anthocyanin galactosylation in purple celery. Celery is a well-known vegetable because of its rich nutrients, low calories, and medicinal values. Its petioles and leaf blades are the main organs acting as nutrient sources. UDP-galactose: cyanidin 3-O-galactosyltransferase can transfer the galactosyl moiety from UDP-galactose to the 3-O-position of cyanidin through glycosylation. This process enhances the stability and water solubility of anthocyanins...
March 8, 2018: Planta
Katarzyna Bilska, Kinga Stuper-Szablewska, Tomasz Kulik, Maciej Buśko, Dariusz Załuski, Sebastian Jurczak, Juliusz Perkowski
Flavonoids are a group of hydroxylated polyphenolic compounds widely distributed in the plant kingdom. Biosynthesis of these compounds involves type III PKSs, whose presence has been recently predicted in some fungal species through genome sequencing efforts. In this study, for the first time it was found that Fusaria produce flavonoids on solid YES medium. Naringenin, as the central precursor of all flavonoids, was produced at highest quantities, followed by quercetin, kaempferol, apigenin and luteolin. In plants, flavonoids are involved in the protection of cereals to a wide range of stresses, including host defense against Fusaria...
March 5, 2018: Toxins
Gokhan Zengin, Devina Lobine, Adriano Mollica, Marcello Locatelli, Simone Carradori, Mohamad Fawzi Mahomoodally
The ethyl acetate, methanolic, and water extracts of Fibigia eriocarpa were assessed for a panoply of bioactivities. Total phenolic and flavonoid content were quantified as well as individual phenolic compounds by HPLC-DAD. The in-vitro antioxidant and enzyme (acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, α-amylase, and α-glucosidase) inhibitory potential of the extracts were evaluated. In silico molecular docking was used to investigate possible interaction between dominant compounds and selected enzymes...
March 5, 2018: Fundamental & Clinical Pharmacology
Jiangdi Liu, Na Chen, Jian Yang, Bin Yang, Zhen Ouyang, Changxun Wu, Yuan Yuan, Weihao Wang, Min Chen
In the present study, an integrated approach combining HPLC/DAD, GC/MS, near infrared (NIR) spectroscopy, and chemometrics was used to geographically discriminate saffron samples from Iran and China. Chinese and Iranian samples can be well-separated based on HPLC data analysed by a principal component analysis and an orthogonal partial least squares discriminant analysis. Picrocrocin and two types of crocins were found to be the discriminating variables, and the Chinese samples had higher contents of safranal and picrocrocin but lower cis-crocin 3Gg, kaempferol-3-O-sophoroside and isophorone...
July 1, 2018: Food Chemistry
Lucas Olivo Martins Pereira, Wagner Vilegas, Marcelo Marucci Pereira Tangerina, Karuppusamy Arunachalam, Sikiru Olaitan Balogun, Paulo Eduardo Orlandi Matos, Domingos Tabajara de Oliveira Martins
ETHNOPHARMACOLOGICAL IMPORTANCE: Lafoensia pacari A. St.-Hil., (Lythraceae) is a native tree of Brazilian Cerrado and commonly known in Brazil as "mangava-brava". Its leaves are used in Brazilian folk medicine in wound healing, cutaneous mycoses, and in the treatment of gastritis and ulcers. AIM OF THE STUDY: The present study was designed to evaluate the wound healing activity and mechanism of action of the hydroethanolic extract of Lafoensia pacari A...
February 28, 2018: Journal of Ethnopharmacology
Sean M Bugel, Robert L Tanguay
The comparative analysis of complex behavioral phenotypes is valuable as a reductionist tool for both drug discovery and defining chemical bioactivity. Flavonoids are a diverse class of chemicals that elicit robust neuroactive and hormonal actions, though bioactivity information is limited for many, particularly for neurobehavioral endpoints. Here, we used a zebrafish larval chemomotor response (LCR) bioassay to comparatively evaluate a suite of 24 flavonoids, and in addition a panel of 30 model neuroactive compounds representing diverse modes of action (e...
February 27, 2018: Toxicology and Applied Pharmacology
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