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https://www.readbyqxmd.com/read/28330214/production-kinetics-of-polyhydroxyalkanoates-by-using-pseudomonas-aeruginosa-gamma-ray-mutant-strain-ebn-8-cultured-on-soybean-oil
#1
Sharjeel Abid, Zulfiqar Ali Raza, Tanveer Hussain
The purpose of present study was to optimize polyhydroxyalkanotes (PHAs) production in a gamma ray mutant strain of Pseudomonas aeruginosa grown on soybean oil in minimal salts media under shake flask conditions. The production kinetics was studied by sampling on daily basis for 6 days to investigate the best conditions for PHAs production like biomass estimation, carbon source utilization and PHAs yield. The PHA accumulation was observed up to 50.27 % (w/w) of cell dry mass. The Pseudomonas species synthesized medium chain length PHA copolyester as per identified by LCMS and confirmed by FTIR spectroscopy...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28322160/an-efficient-transient-expression-system-for-enhancing-generation-of-monoclonal-antibodies-in-293-suspension-cells
#2
Guoquan Zhang, Jie Liu, Wanting Fan, Qianyi Chen, Bin Shi
BACKGROUND: Recombinant monoclonal antibodies (mAbs) are useful in research, diagnosis, and therapy. The increased demands of recombinant mAbs require efficient production systems. A variety of expression vectors have been developed for stable or transient production of mAb in mammalian cells. However, though a few commercial expression systems of mAbs can be listened, the high expense often impedes academia researches. METHODS: In this study, we described the development of a transient mammalian system based on a bicistronic vector, which contains internal ribosome entry site (IRES) and enhancer elements to express IgG1 light chain (LC) and heavy chain (HC) in one transcript...
March 20, 2017: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/28319778/polyhydroxyalkanoates-pha-production-from-synthetic-waste-using-pseudomonas-pseudoflava-pha-synthase-enzyme-activity-analysis-from-p-pseudoflava-and-p-palleronii
#3
M Venkateswar Reddy, Yasuteru Mawatari, Rui Onodera, Yuki Nakamura, Yuka Yajima, Young-Cheol Chang
Synthetic wastewater (SW) at various carbon concentrations (5-60g/l) were evaluated for polyhydroxyalkanoates (PHA) production using the bacteria Pseudomonas pseudoflava. Bacteria showed highest PHA production with 20g/l (57±5%), and highest carbon removal at 5g/l (74±6%) concentrations respectively. Structure, molecular weight, and thermal properties of the produced PHA were evaluated using various analytical techniques. Bacteria produced homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate was used as carbon source; and it produced co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate...
March 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28315876/angiotensin-converting-enzyme-ins-del-polymorphism-and-body-composition-the-intermediary-role-of-hydration-status
#4
Laura Bordoni, Valerio Napolioni, Francesca Marchegiani, Emilio Amadio, Rosita Gabbianelli
BACKGROUND: The well-known insertion/deletion polymorphism (rs4646994) of the angiotensin-converting enzyme (ACE) gene has been previously associated with obesity, blood flow, muscular strength, and ACE enzyme activity. Despite the relevant role of ACE in homeostasis, few data are currently available on the relationship between rs4646994 and hydration status. Thus, we tested the association between the ACE Ins/Del polymorphism, body composition, and hydration status in a young Italian population...
March 18, 2017: Journal of Nutrigenetics and Nutrigenomics
https://www.readbyqxmd.com/read/28314208/long-term-effects-of-sulphide-on-the-enhanced-biological-removal-of-phosphorus-the-symbiotic-role-of-thiothrix-caldifontis
#5
F J Rubio-Rincón, L Welles, C M Lopez-Vazquez, M Nierychlo, B Abbas, M Geleijnse, P H Nielsen, M C M van Loosdrecht, D Brdjanovic
Thiothrix caldifontis was the dominant microorganism (with an estimated bio-volume of 65 ± 3%) in a lab-scale enhanced biological phosphorus removal (EBPR) system containing 100 mg of sulphide per litre in the influent. After a gradual exposure to the presence of sulphide, the EBPR system initially dominated by Candidatus Accumulibacter phosphatis Clade I (98 ± 3% bio-volume) (a known polyphosphate accumulating organism, PAO) became enriched with T. caldifontis. Throughout the different operating conditions studied, practically 100% phosphate removal was always achieved...
March 7, 2017: Water Research
https://www.readbyqxmd.com/read/28306177/frequency-and-functional-characterization-of-exhausted-cd8-t-cells-in-chronic-lymphocytic-leukemia
#6
Saeid Taghiloo, Esmaeil Allahmoradi, Mohsen Tehrani, Hadi Hossein-Nataj, Ramin Shekarriz, Ghasem Janbabaei, Saeid Abediankenari, Hossein Asgarian-Omran
OBJECTIVES: The phenotypic and functional properties of Tim-3+/PD-1+/CD8+ cells as exhausted T-cells were investigated in chronic lymphocytic leukemia (CLL). METHODS: Frequency of CD8+/Tim-3+/PD-1+ exhausted cells was determined by flow cytometry. For functional analysis, magnetic beads isolated CD8+ T-cells were stimulated with PHA and PMA/ionocymin to assess their proliferative responses and cytokine production by MTT and ELISA, respectively. Cytotoxic activity of isolated CD8+ T-cells was determined using CD107a degranulation assay...
March 17, 2017: European Journal of Haematology
https://www.readbyqxmd.com/read/28294212/modulation-of-the-electronic-and-mechanical-properties-of-phagraphene-via-hydrogenation-and-fluorination
#7
Donghai Wu, Shuaiwei Wang, Jinyun Yuan, Baocheng Yang, Houyang Chen
Recently, a new carbon sheet, phagraphene, was proposed by theoretical calculations [Nano Lett. 2015, 15, 6182]. In this paper, hydrogenated and fluorinated phagraphene (denoted as H-PHA and F-PHA) sheets have been systematically studied using first-principles calculations. The results of formation energy, ab initio molecular dynamics, phonon dispersion and elastic constants confirm that the modified phagraphene sheets are thermodynamically and dynamically as well as mechanically stable. We find that hydrogenation or fluorination is an effective way to modulate the bandgap, and we also find that adsorption-induced semimetal-semiconductor transition and adsorption-induced semimetal-insulator transition occur...
March 15, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28284546/accumulation-of-coal-combustion-residues-and-their-immunological-effects-in-the-yellow-bellied-slider-trachemys-scripta-scripta
#8
David L Haskins, Matthew T Hamilton, Amanda L Jones, John W Finger, Robert B Bringolf, Tracey D Tuberville
Anthropogenic activities such as industrial processes often produce copious amounts of contaminants that have the potential to negatively impact growth, survival, and reproduction of exposed wildlife. Coal combustion residues (CCRs) represent a major source of pollutants globally, resulting in the release of potentially harmful trace elements such as arsenic (As), cadmium (Cd), and selenium (Se) into the environment. In the United States, CCRs are typically stored in aquatic settling basins that may become attractive nuisances to wildlife...
March 8, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28283932/characterization-of-polyhydroxyalkanoate-produced-by-bacillus-megaterium-vb89-isolated-from-nisargruna-biogas-plant
#9
Vishakha Baikar, Ashwini Rane, Rajendra Deopurkar
Polyhydroxyalkanoates (PHAs) are naturally occurring biodegradable polymers that can curb the extensive use of polypropylene based plastics. In contrast to chemically synthesized polypropylene plastics, PHAs are biodegradable and thus environmentally safe. PHAs have attracted much attention as biocompatible and biodegradable thermoplastics. The present study involves isolation of bacteria from different environments capable of synthesizing PHAs. The bacterium producing highest yield of PHA (0.672 ± 0.041 g/L) was identified as Bacillus megaterium VB89 by biochemical and molecular techniques such as 16S rDNA sequence analysis...
March 10, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28274100/production-of-polyhydroxyalkanoates-from-sludge-palm-oil-using-pseudomonas-putida-s12
#10
Du-Kyeong Kang, Cho-Ryong Lee, Sun Hee Lee, Jung-Hoon Bae, Young-Kwon Park, Young Ha Rhee, Bong Hyun Sung, Jung-Hoon Sohn
Polyhydroxyalkanoates (PHAs) are biodegradable plastics produced by bacteria, but their use in diverse applications is prohibited by high production costs. To reduce these costs, the conversion of PHAs from crude sludge palm oil (SPO) as an inexpensive renewable raw material by Pseudomonas strains was tested. Pseudomonasputida S12 was found to produce the highest yield (~41%) of elastomeric medium-chain-length (MCL)-PHAs from SPO. The MCL-PHA characteristics were analyzed by gas-chromatography/mass spectrometry, gel permeation chromatography, and differential scanning calorimetry...
March 9, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28273463/reversal-of-ddk-mediated-mcm-phosphorylation-by-rif1-pp1-regulates-replication-initiation-and-replisome-stability-independently-of-atr-chk1
#11
Robert C Alver, Gaganmeet Singh Chadha, Peter J Gillespie, J Julian Blow
Dbf4-dependent kinases (DDKs) are required for the initiation of DNA replication, their essential targets being the MCM2-7 proteins. We show that, in Xenopus laevis egg extracts and human cells, hyper-phosphorylation of DNA-bound Mcm4, but not phosphorylation of Mcm2, correlates with DNA replication. These phosphorylations are differentially affected by the DDK inhibitors PHA-767491 and XL413. We show that DDK-dependent MCM phosphorylation is reversed by protein phosphatase 1 (PP1) targeted to chromatin by Rif1...
March 7, 2017: Cell Reports
https://www.readbyqxmd.com/read/28271177/immune-challenges-and-visual-signalling-in-tree-frogs
#12
Julia L Desprat, Thierry Lengagne, Nathalie Mondy
In animals, mate-choice is often based on sexual signals that carry information and help the receiver make the best choice to improve the receiver's fitness. Orange visual sexual signals have been hypothesised to carry immune information because they are often due to carotenoid pigments which are also involved in immunity response. Although many studies have focused on the direct relationships between coloration and immunocompetence, few studies have simultaneously studied immunocompetent response and coloration variation after an immune challenge...
April 2017: Die Naturwissenschaften
https://www.readbyqxmd.com/read/28259067/multi-cycle-operation-of-enhanced-biological-phosphorus-removal-ebpr-with-different-carbon-sources-under-high-temperature
#13
Nan Shen, Yun Chen, Yan Zhou
Many studies reported that it is challenging to apply enhanced biological phosphorus removal (EBPR) process at high temperature. Glycogen accumulating organisms (GAOs) could easily gain their dominance over poly-phosphate accumulating organisms (PAOs) when the operating temperature was in the range of 25 °C-30 °C. However, a few successful EBPR processes operated at high temperature have been reported recently. This study aimed to have an in-depth understanding on the impact of feeding strategy and carbon source types on EBPR performance in tropical climate...
February 22, 2017: Water Research
https://www.readbyqxmd.com/read/28254060/effects-of-sludge-retention-time-carbon-and-initial-biomass-concentrations-on-selection-process-from-activated-sludge-to-polyhydroxyalkanoate-accumulating-cultures
#14
Zhiqiang Chen, Long Huang, Qinxue Wen, Huichao Zhang, Zirui Guo
Four sequence batch reactors (SBRs) fed by fermented sugar cane wastewater were continuously operated under the aerobic dynamic feeding (ADF) mode with different configurations of sludge retention time (SRT), carbon and initial biomass concentrations to enrich polyhydroxyalkanoate (PHA) accumulating mixed microbial cultures (MMCs) from municipal activated sludge. The stability of SBRs was investigated besides the enrichment performance. The microbial community structures of the enriched MMCs were analyzed using terminal restriction fragment length polymorphism (T-RFLP)...
February 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28249922/clinical-manifestation-and-molecular-analysis-of-three-korean-patients-with-the-renal-form-of-pseudohypoaldosteronism-type-1
#15
Hyo-Kyoung Nam, Myung-Hyun Nam, Hye Ryun Kim, Young-Jun Rhie, Kee Hwan Yoo, Kee-Hyoung Lee
Pseudohypoaldosteronism (PHA) type 1 is a rare, heterogeneous disease characterized by hyponatremia and hyperkalemia due to mineralocorticoid resistance. The clinical features of PHA are usually failure to thrive, vomiting, and dehydration in the neonatal period. Heterozygous mutations in the Nuclear receptor subfamily 3, group C, member 2 (NR3C2) gene result in the dominant renal form of PHA type 1. Mutations in the epithelial sodium channel gene result in the more severe, recessive, systemic form of PHA type 1...
January 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/28246274/proliferation-markers-are-associated-with-met-expression-in-hepatocellular-carcinoma-and-predict-tivantinib-sensitivity-in-vitro
#16
Sandra Rebouissou, Tiziana La Bella, Samia Rekik, Sandrine Imbeaud, Anna-Line Calatayud, Nataliya Rorh-Udilova, Yoann Martin, Gabrielle Couchy, Paulette Bioulac-Sage, B Grasl-Kraupp, Leanne de Koning, Nathalie Ganne-Carrie, Jean-Charles Nault, Marianne Ziol, Jessica Zucman-Rossi
PURPOSE: Tivantinib was initially reported as a selective MET inhibitor and is under phase 3 evaluation in "MET-high" hepatocellular carcinoma (HCC) patients. However, it has been also proposed as an antimitotic agent. We aimed to evaluate the anti-tumor effect of tivantinib in HCC cells by combining pharmacological and molecular profiling. EXPERIMENTAL DESIGN: Sensitivity to tivantinib, JNJ-38877605, PHA-665752, vinblastine and paclitaxel was tested in a panel of 35 liver cancer cell lines analyzed with exome sequencing, mRNA expression of 188 genes and protein expression...
February 28, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28237496/effect-of-acetate-as-a-co-feedstock-on-the-production-of-poly-lactate-co-3-hydroxyalkanoate-by-pfla-deficient-escherichia-coli-rsc10
#17
Lucia Salamanca-Cardona, Ryan A Scheel, Kouhei Mizuno, N Scott Bergey, Arthur J Stipanovic, Ken'ichiro Matsumoto, Seiichi Taguchi, Christopher T Nomura
Developing Escherichia coli strains that are tolerant to acetate toxicity is important in light of an increased interest in the efficient utilization of lignocellulosic biomass feedstocks for the biosynthesis of value-added products. In this study, four strains known to produce polyhydroxyalkanoates (PHAs) from the typical hemicellulosic sugar xylose were tested for their tolerance to acetate. E. coli RSC10 was found to be tolerant of acetate, both in growth and fermentation studies. In the presence of acetate the strain showed a >2-fold increase in overall yields compared to using xylose alone as the feedstock...
February 22, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28235702/design-synthesis-biological-evaluation-and-x-ray-structural-studies-of-potent-human-dihydroorotate-dehydrogenase-inhibitors-based-on-hydroxylated-azole-scaffolds
#18
Stefano Sainas, Agnese C Pippione, Marta Giorgis, Elisa Lupino, Parveen Goyal, Cristina Ramondetti, Barbara Buccinnà, Marco Piccinini, Rodolpho C Braga, Carolina H Andrade, Mikael Andersson, Ann-Christin Moritzer, Rosmarie Friemann, Stefano Mensa, Salam Al-Kadaraghi, Donatella Boschi, Marco L Lolli
A new generation of potent hDHODH inhibitors designed by a scaffold-hopping replacement of the quinolinecarboxylate moiety of brequinar, one of the most potent known hDHODH inhibitors, is presented here. Their general structure is characterized by a biphenyl moiety joined through an amide bridge with an acidic hydroxyazole scaffold (hydroxylated thiadiazole, pyrazole and triazole). Molecular modelling suggested that these structures should adopt a brequinar-like binding mode involving interactions with subsites 1, 2 and 4 of the hDHODH binding site...
March 31, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28229643/vestpd-as-a-measure-of-ventilatory-acclimatization-to-hypobaric-hypoxia
#19
J A Loeppky, A C Sheard, R M Salgado, C M Mermier
This study compared the ventilation response to an incremental ergometer exercise at two altitudes: 633 mmHg (resident altitude = 1,600 m) and following acute decompression to 455 mmHg (≈4,350 m altitude) in eight male cyclists and runners. At 455 mmHg, the VESTPD at RER <1.0 was significantly lower and the VEBTPS was higher because of higher breathing frequency; at VO2max, both VESTPD and VEBTPS were not significantly different. As percent of VO2max, the VEBTPS was nearly identical and VESTPD was 30% lower throughout the exercise at 455 mmHg...
September 2016: Physiol Int
https://www.readbyqxmd.com/read/28222027/vitamin-d-modulates-the-expression-of-hla-dr-and-cd38-after-in-vitro-activation-of-t-cells
#20
Simon Villegas-Ospina, Wbeimar Aguilar-Jimenez, Sandra M Gonzalez, María T Rugeles
OBJECTIVE: Vitamin D (VitD) is an anti-inflammatory hormone; however, some evidence shows that VitD may induce the expression of activation markers, such as CD38 and HLA-DR. We explored its effect on the expression of these markers on CD4+ and CD8+ T-cells in vitro, and their potential correlations in vivo. MATERIALS AND METHODS: CD38 and HLA-DR expression was measured by flow cytometry in PHA/IL-2-activated mononuclear cells cultured under VitD precursors: three cholecalciferol (10-11M, 10-9M, 10-7M; n=11) and two calcidiol (40 ng/mL, 80 ng/mL; n=9) concentrations...
March 1, 2017: Hormone Molecular Biology and Clinical Investigation
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