keyword
MENU ▼
Read by QxMD icon Read
search

pha

keyword
https://www.readbyqxmd.com/read/28539408/temporal-regulation-of-epithelium-formation-mediated-by-foxa-mklp1-mgcracgap-and-par-6
#1
Stephen E Von Stetina, Jennifer Liang, Georgios Marnellos, Susan E Mango
To establish the animal body plan, embryos link the external epidermis to the internal digestive tract. In Caenorhabditis elegans, this linkage is achieved by the Arcade Cells, which form an epithelial bridge between the foregut and epidermis, but little is known about how development of these three epithelia is coordinated temporally. The Arcade Cell epithelium is generated after the epidermis and digestive tract epithelia have matured, ensuring that both organs can withstand the mechanical stress of embryo elongation; mis-timing of epithelium formation leads to defects in morphogenesis...
May 24, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28532241/bacillus-cereus-type-polyhydroxyalkanoate-biosynthetic-gene-cluster-contains-r-specific-enoyl-coa-hydratase-gene
#2
Takahiro Kihara, Ayaka Hiroe, Manami Ishii-Hyakutake, Kouhei Mizuno, Takeharu Tsuge
Bacillus cereus and Bacillus megaterium both accumulate polyhydroxyalkanoate (PHA) but their PHA biosynthetic gene (pha) clusters that code for proteins involved in PHA biosynthesis are different. Namely, a gene encoding MaoC-like protein exists in the B. cereus-type pha cluster but not in the B. megaterium-type pha cluster. MaoC-like protein has an R-specific enoyl-CoA hydratase (R-hydratase) activity and is referred to as PhaJ when involved in PHA metabolism. In this study, the pha cluster of B. cereus YB-4 was characterized in terms of PhaJ's function...
May 22, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28531943/recent-trends-in-zikv-research-a-step-away-from-cure
#3
REVIEW
Aftab Alam, Nikhat Imam, Anam Farooqui, Shahnawaz Ali, Md Zubbair Malik, Romana Ishrat
Zika virus (ZikV) is a member of the Flaviviridae virus family, genus Flavivirus has emerged as a potential threat to human health worldwide. Consequences of vertical infections includes microcephaly with brain and eye anomalies, and adult infections includes Guillain-Barrésyndrome (GBS), brain ischemia, myelitis and meningoencephalitis. To develop a better treatment, many efforts are being made, like drug-repurposing concept for FDA-approved drugs for antiviral activity are screened against ZikV infection and emerging as a promising alternative to expedite drug development and various vaccines like DNA, ZPIV, LAIV, mRNA and AGS-v vaccines have been designed and in under clinical trial phases...
May 17, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28529954/%C3%AE-7-nicotinic-acetylcholine-receptor-stimulation-attenuates-neuroinflammation-through-jak2-stat3-activation-in-murine-models-of-intracerebral-hemorrhage
#4
Paul R Krafft, Devin McBride, William B Rolland, Tim Lekic, Jerry J Flores, John H Zhang
Accounting for high mortality and morbidity rates, intracerebral hemorrhage (ICH) remains one of the most detrimental stroke subtypes lacking a specific therapy. Neuroinflammation contributes to ICH-induced brain injury and is associated with unfavorable outcomes. This study aimed to evaluate whether α7 nicotinic acetylcholine receptor (α7nAChR) stimulation ameliorates neuroinflammation after ICH. Male CD-1 mice and Sprague-Dawley were subjected to intracerebral injection of autologous blood or bacterial collagenase...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28528947/syntrophic-association-of-termite-gut-bacterial-symbionts-with-bifunctional-characteristics-of-cellulose-degrading-and-polyhydroxyalkanoate-producing-bacteria
#5
Balasubramanian Cibichakravarthy, Subramani Abinaya, Solai Ramatchandirane Prabagaran
The guild between higher termites and their partnership with the diverse community of bacteria and archaea in their gut is a marvel evolutionary achievement. Sustained attempts were made worldwide with a quest for identifying viable important biological macromolecule polyhydroxyalkanoate (PHA) accumulating bacteria. Termite gut serve as a novel source for bacteria with dual properties like PHA production as well as cellulose degradation. Among 40 isolates cultivated, 32.5% turned positive for PCR based screening of PhaC gene...
May 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28527955/neuroprotective-effect-of-the-alpha-7-nicotinic-receptor-agonist-pha-543613-in-an-in-vivo-excitotoxic-adult-rat-model
#6
Laura Foucault-Fruchard, Aurélie Doméné, Guylène Page, Marguerite Windsor, Patrick Emond, Nuno Rodrigues, Frédéric Dollé, Annelaure Damont, Frédéric Buron, Sylvain Routier, Sylvie Chalon, Daniel Antier
Neuroinflammation is a key component of the pathophysiology of neurodegenerative diseases. The link between nicotine intake and positive outcome has been established, suggesting a role played by nicotinic receptors (nAChRs), especially α7nAChRs. The objective of this study was to evaluate the potential dose effects of PHA 543613 on neuron survival and striatal microglial activation in a rat model of brain excitotoxicity. A preliminary study was performed in vitro to confirm PHA 543613 agonist properties on α7nAChRs...
May 17, 2017: Neuroscience
https://www.readbyqxmd.com/read/28527688/clinical-development-of-a-var2csa-based-placental-malaria-vaccine-pamvac-quantifying-vaccine-antigen-specific-memory-b-t-cell-activity-in-beninese-primigravidae
#7
Komi Gbédandé, Nadine Fievet, Firmine Viwami, Sem Ezinmegnon, Saadou Issifou, Jean-Philippe Chippaux, Yannelle Dossou, Kabirou Moutairou, Achille Massougbodji, Nicaise Ndam, Willem Adriaan de Jongh, T Max M Søgaard, Ali Salanti, Morten A Nielsen, Meral Esen, Benjamin Mordmüller, Philippe Deloron, Adrian J F Luty
BACKGROUND: The antigen VAR2CSA plays a pivotal role in the pathophysiology of pregnancy-associated malaria (PAM) caused by Plasmodium falciparum. A VAR2CSA-based vaccine candidate, PAMVAC, is under development by an EU-funded multi-country consortium (PlacMalVac project). As part of PAMVAC's clinical development, we quantified naturally acquired vaccine antigen-specific memory B and T cell responses in Beninese primigravidae recruited at the beginning of pregnancy and followed up to delivery and beyond...
May 17, 2017: Vaccine
https://www.readbyqxmd.com/read/28527342/relative-efficacy-of-t-cell-stimuli-as-inducers-of-productive-hiv-1-replication-in-latently-infected-cd4-lymphocytes-from-patients-on-suppressive-cart
#8
Nadejda Beliakova-Bethell, Marjan Hezareh, Joseph K Wong, Matthew C Strain, Mary K Lewinski, Douglas D Richman, Celsa A Spina
Quantification of cell-associated replication-competent HIV, in blood samples from patients with undetectable plasma viremia, requires specialized culture conditions that include exogenous pan T cell stimulation. Different research groups have used several stimuli for this purpose; however, the relative efficacies of these T cell stimuli to induce productive HIV replication from latently infected cells ex vivo have not been systematically evaluated. To this end, we compared four commonly used T cell stimuli: 1) irradiated allogeneic cells plus phytohaemagglutinin (PHA); 2) PHA alone; 3) phorbol myristate acetate plus Ionomycin; and 4) immobilized αCD3 plus αCD28 antibodies...
May 17, 2017: Virology
https://www.readbyqxmd.com/read/28525335/morphometric-abnormalities-in-the-spleen-of-the-progeny-of-mice-fed-epigallocatechin-during-gestation-and-nursing
#9
B J Bałan, E Skopińska-Różewska, P Skopiński, R Zdanowski, M Leśniak, A Kiepura, S Lewicki
It is very difficult to cure pregnant females suffering from infections, because of the risk which might occur during treatment by several, even herbal, medications. Many of these substances, among them extracts from plants, have antimicrobial, anti-inflammatory and immunostimulatory properties owing to their polyphenols content, but also may reveal unwanted effects on the fetal development because of their anti-angiogenic properties. The aim of the present study was to elucidate whether daily feeding pregnant and nursing mice 0...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28521993/chitosan-oligosaccharides-inhibit-epithelial-cell-migration-through-blockade-of-n-acetylglucosaminyltransferase-v-and-branched-glcnac-structure
#10
Qingsong Xu, Wenjing Wang, Chen Qu, Jianguo Gu, Heng Yin, Zhihao Jia, Linsheng Song, Yuguang Du
Chitosan oligosaccharides (COS) have been shown to regulate various cellular and biological functions. The aim of this study was to investigate the antimetastatic potency of COS and the underlying mechanism. Here, we established a stably N-acetylglucosaminyltransferase V (GnT-V)-overexpressed MCF10A cell line. As expected, GnT-V overexpression greatly promoted cell migration in the transfectants by using wound healing assay. However, the induction in the cell migration was significantly suppressed by an addition of COS...
August 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28521235/integrated-bioconversion-of-syngas-into-bioethanol-and-biopolymers
#11
Borja Lagoa-Costa, Haris Nalakath Abubackar, María Fernández-Romasanta, Christian Kennes, María C Veiga
Syngas bioconversion is a promising method for bioethanol production, but some VFA remains at the end of fermentation. A two-stage process was set-up, including syngas fermentation as first stage under strict anaerobic conditions using C. autoethanogenum as inoculum, with syngas (CO/CO2/H2/N2, 30/10/20/40) as gaseous substrate. The second stage consisted in various fed-batch assays using a highly enriched PHA accumulating biomass as inoculum, where the potential for biopolymer production from the remaining acetic acid at the end of the syngas fermentation was evaluated...
May 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28516133/transcatheter-arterial-chemoembolization-of-hepatocellular-carcinoma-in-patients-with-celiac-axis-occlusion-using-pancreaticoduodenal-arcade-as-a-challenging-alternative-route
#12
Noha M Attia, Moustafa H M Othman
INTRODUCTION: Celiac axis occlusion is a challenging condition when catheterization of the hepatic artery is required for chemoembolization of hepatocellular carcinoma (HCC). As a result, the hepatic artery has to be catheterized through the pancreaticoduodenal arcades (PDA) and the gastroduodenal artery (GDA) from the superior mesenteric artery (SMA) which is a tortuous course with acute angles and small caliber branches. OBJECTIVE: To assess new techniques for facilitating catheterization of the tortuous PDA and the GDA to reach the proper hepatic artery (PHA) and tumor-feeding branches in patients with celiac axis occlusion undergoing chemoembolization of HCC...
2017: European Journal of Radiology Open
https://www.readbyqxmd.com/read/28511586/bioprocess-optimization-of-phb-homopolymer-and-copolymer-p3-hb-co-hv-by-acinetobacter-junii-bp25-utilizing-rice-mill-effluent-as-sustainable-substrate
#13
S Poorna Chandrika, D Sabarinathan, P Anburajan, K Preethi
The potential use of parboiled rice mill effluent as a cheap substrate for the production of homopolymer and copolymer of Polyhydroxyalkanoates (PHAs) by Acinetobacter junii BP 25 was investigated for the first time. Process optimization by one factor at a time led to homopolymer polyhydroxybutyrate (PHB) production of 2.64 ± 0.18 g/l with 94.28% PHB content using a two stage batch cultivation mode. BP 25 furthermore produced polyhydroxybutyrate-co-hydroxyvalerate (P3 (HB-co-HV)), with the addition of valeric acid as an additive to the substrate, yielding (2...
May 16, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28510128/specific-character-of-bacterial-biodegradation-of-polyhydroxyalkanoates-with-different-chemical-structure-in-soil
#14
S V Prudnikova, O N Vinogradova, M Y Trusova
The study addresses the influence of the physicochemical properties of the reserve cellular macromolecules (polyhydroxyalkanoates, PHA) with different chemical composition on their biodegradation in the agro-transformed field soil of the Siberian region (Krasnoyarsk Territory, Russia). It was shown that the degradation of the PHA samples depends on the degree of polymer crystallinity (Cx). For the first time, it was shown that the range of PHA-degrading microorganisms differs for each of PHA types. The study defines the primary degraders specific to each PHA type and common to all types of examined polymers...
March 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28508433/an-investigation-of-the-anti-inflammatory-effects-and-a-potential-biomarker-of-pi3k%C3%AE-inhibition-in-copd-t-cells
#15
Abid Khan, Thomas Southworth, Sally Worsley, Srividya Sriskantharajah, Augustin Amour, Edith M Hessel, Dave Singh
Lymphocyte numbers are increased in the lungs of chronic obstructive pulmonary disease (COPD) patients. Phosphatidylinositol-3-kinase delta (PI3Kδ) is involved in lymphocyte activation. We investigated the effect of PI3Kδ inhibition on cytokine release from COPD lymphocytes. We also evaluated phosphorylated ribosomal S6 protein (rS6) as a potential biomarker of PI3Kδ activation. Peripheral blood mononuclear cells (PBMCs) and bronchoalveolar lavage (BAL) cells isolated from healthy never smokers (HNS), smokers (S) and COPD patients were stimulated to induce a T cell receptor response...
May 15, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28508279/the-coexistence-of-both-replaced-proper-hepatic-and-gastroduodenal-arteries-due-to-the-common-hepatic-artery-absence
#16
Konstantinos Natsis, Maria Piagkou, Nikolaos Lazaridis, Georgios Koimtzis, Stylianos Apostolidis
Anatomical variants of the celiac trunk (CT) branches and especially these of hepatic arteries (HAs) are among the most common variants of the arterial tree. The knowledge of the wide variability in hepatic arterial supply is of paramount importance in hepatobiliary, pancreatic, gastric, and esophageal surgery, as well as in liver transplantations. The purpose of this case report is to describe a rare variant discovered during abdominal dissection of a 74-year-old male cadaver of Greek origin, in which the common hepatic artery was absent and its branches, the proper hepatic artery (PHA) and the gastroduodenal artery (GDA) had an aberrant and separate origin...
May 15, 2017: Surgical and Radiologic Anatomy: SRA
https://www.readbyqxmd.com/read/28507540/microbial-population-dynamics-and-ecosystem-functions-of-anoxic-aerobic-granular-sludge-in-sequencing-batch-reactors-operated-at-different-organic-loading-rates
#17
Enikö Szabó, Raquel Liébana, Malte Hermansson, Oskar Modin, Frank Persson, Britt-Marie Wilén
The granular sludge process is an effective, low-footprint alternative to conventional activated sludge wastewater treatment. The architecture of the microbial granules allows the co-existence of different functional groups, e.g., nitrifying and denitrifying communities, which permits compact reactor design. However, little is known about the factors influencing community assembly in granular sludge, such as the effects of reactor operation strategies and influent wastewater composition. Here, we analyze the development of the microbiomes in parallel laboratory-scale anoxic/aerobic granular sludge reactors operated at low (0...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28502064/optimization-of-water-oil-surfactant-system-for-preparation-of-medium-chain-length-poly-3-hydroxyalkanoates-mcl-pha-incorporated-nanoparticles-via-nanoemulsion-templating-technique
#18
K A Ishak, M Suffian M Annuar, N Ahmad
Polymeric nanoparticles gain a widespread interest in food and pharmaceutical industries as delivery systems that encapsulate, protect, and release lipophilic compounds such as omega-3 fatty acids, fat-soluble vitamins, carotenoids, carvedilol, cyclosporine, and ketoprofen. In this study, medium-chain-length poly-3-hydroxyalkanoate (mcl-PHA)-incorporated nanoparticle was developed via facile organic solvent-free nanoemulsion templating technique. The water content (W/surfactant-to-oil (S/O)), S/O, and Cremophor EL-to-Span 80 (Cremo/Sp80) ratios were first optimized using response surface methodology (RSM) to obtain nanoemulsion template prior to incorporation of mcl-PHA...
May 13, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28500546/quantitative-image-analysis-of-polyhydroxyalkanoates-inclusions-from-microbial-mixed-cultures-under-different-sbr-operation-strategies
#19
António L Amaral, Hugo Abreu, Cristiano Leal, Daniela P Mesquita, Luís M Castro, Eugénio C Ferreira
Polyhydroxyalkanoates (PHAs) produced from mixed microbial cultures (MMC), regarded as potential substitutes of petrochemical plastics, can be found as intracellular granules in various microorganisms under limited nutrient conditions and excess of carbon source. PHA is traditionally quantified by laborious and time-consuming chromatography analysis, and a simpler and faster method to assess PHA contents from MMC, such as quantitative image analysis (QIA), is of great interest. The main purpose of the present work was to upgrade a previously developed QIA methodology (as reported by Mesquita et al...
May 12, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28497282/highly-complex-substrates-lead-to-dynamic-bacterial-community-for-polyhydroxyalkanoates-production
#20
Diogo Queirós, Alexandre Fonseca, Simona Rossetti, Luísa S Serafim, Paulo C Lemos
Mixed microbial cultures (MMC) and waste/surplus substrates, as hardwood spent sulfite liquor, are being used to decrease polyhydroxyalkanoates' (PHA) production costs. The process involves two or three steps, being the selection step a crucial one. For the industrial implementation of this strategy, reactor stability in terms of both performance and microbial community presence has to be considered. A long-term operation of a sequencing batch reactor under feast/famine conditions was performed along with microbial community identification/quantification using FISH and DGGE...
May 11, 2017: Journal of Industrial Microbiology & Biotechnology
keyword
keyword
17154
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"