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https://www.readbyqxmd.com/read/28425176/polyhydroxyalkanoate-associated-phasins-as-phylogenetically-heterogeneous-multipurpose-proteins
#1
REVIEW
Beatriz Maestro, Jesús M Sanz
Polyhydroxyalkanoates (PHAs) are natural polyesters of increasing biotechnological importance that are synthesized by many prokaryotic organisms as carbon and energy storage compounds in limiting growth conditions. PHAs accumulate intracellularly in form of inclusion bodies that are covered with a proteinaceous surface layer (granule-associated proteins or GAPs) conforming a network-like surface of structural, metabolic and regulatory polypeptides, and configuring the PHA granules as complex and well-organized subcellular structures that have been designated as 'carbonosomes'...
April 20, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28419829/characterization-of-polyhydroxyalkanoates-produced-by-synechocystis-salina-from-digestate-supernatant
#2
Adriana Kovalcik, Katharina Meixner, Matthias Mihalic, Wolfgang Zeilinger, Ines Fritz, Werner Fuchs, Pavel Kucharczyk, Franz Stelzer, Bernhard Drosg
The current commercial production of polyhydroxyalkanoates (PHA) is based on heterotrophic bacteria, using organic carbon sources from crops. To avoid the competition with food and feed production, cyanobacteria, metabolising PHA from carbon dioxide can be used. This research focuses on the investigation of the thermal and rheological properties of PHA polymers accumulated by Synechocystis salina, which had been cultivated in digestate supernatant and a mineral medium. The dried bacterial cells had a polymer content of 5...
April 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28414558/th17-inducing-conditions-lead-to-in-vitro-activation-of-both-th17-and-th1-responses-in-behcet-s-disease
#3
Rabia Deniz, Aysın Tulunay-Virlan, Filiz Ture Ozdemir, Ali Ugur Unal, Gulsen Ozen, Fatma Alibaz-Oner, Imren Aydin-Tatli, Gonca Mumcu, Tulin Ergun, Haner Direskeneli
OBJECTIVES: Interleukin-17 (IL-17) has been associated with the pathogenesis of various autoimmune/inflammatory diseases. The aim of this study was to investigate the expression of Th17-related immunity in an innate immunity-dominated vasculitis, namely Behcet's disease (BD). METHODS: Peripheral blood mononuclear cells from 37 patients (age: 38.5 ± 9.8 years) with BD, and 25 healthy controls (HC) (age: 39.1 ± 9.3 years), were cultured in Th17-inducing conditions (IL-6, Phytohemagglutinin (PHA), IL-1β, and IL-23) for 6 days...
April 17, 2017: Immunological Investigations
https://www.readbyqxmd.com/read/28408939/the-immune-effects-of-an-african-traditional-energy-tonic-in-in-vitro-and-in-vivo-models
#4
Mlungisi Ngcobo, Nceba Gqaleni, Vinny Naidoo, Protus Cele
Most of the African traditional medicines (ATM) are formulated as energy tonics to boost and maintain immune defences. In this study, we aimed to evaluate the immune effects of a traditional energy tonic using peripheral blood mononuclear cells (PBMCs), THP-1 monocytes, and bacteria infected rats. When tested in mitogen and peptidoglycan stimulated PBMCs, this energy tonic showed minimal cytotoxicity, while in acute toxicity studies in rats it did not exhibit any significant toxicity at doses up to 2000 mg/mL/kg...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28404223/standardization-of-biopolymer-production-from-seaweed-associative-bacteria
#5
Ramya R, Sangeetha Devi R, Manikandan A, Rajesh Kannan V
Polyhydroxyalkanoate (PHA) is the biodegradable plastic, which is shown the similar properties to the synthetic plastic. Production of PHA is expensive when compared with petrochemical based plastics costs and also hazardous to ecosystem. In order to overcome these drawbacks, some cheaper commercially available carbon sources like rice bran, coconut cake, palm jaggary, etc. can be used. The present study aimed to identify the potential PHA producing bacterial strains from different seaweeds. Based on PHA production and crotonic acid assay, two bacterial strains were screened and designated as M3 and S6 which were found to be efficient PHA producers...
April 9, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28392318/the-performance-of-the-tbag-pha-ratio-in-the-diagnosis-of-active-tb-disease-in-immunocompromised-patients
#6
Munyemana Jean Bosco, Hongyan Hou, Lie Mao, Xiaohui Wu, Kreeti Devi Ramroop, Yanfang Lu, Liyan Mao, Yu Zhou, Ziyong Sun, Feng Wang
OBJECTIVES: The results of the T-SPOT.TB (T-SPOT) assay are reduced in immunocompromised patients with active tuberculosis (ATB), and it is difficult for using T-SPOT results to distinguish ATB from latent tuberculosis infection (LTBI) in this condition. The aim of this study was to determine the performance of the TBAg/PHA ratio in T-SPOT assay in the diagnosis of ATB in immunocompromised patients. METHODS: One hundred and forty three immunocompromised ATB patients and 124 LTBI individuals were diagnosed according to conventional tests and T-SPOT assay...
April 6, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28390902/design-of-y-shaped-targeting-material-for-liposome-based-multifunctional-glioblastoma-targeted-drug-delivery
#7
Zakia Belhadj, Man Ying, Xie Cao, Xuefeng Hu, Changyou Zhan, Xiaoli Wei, Jie Gao, Xiaoyi Wang, Zhiqiang Yan, Weiyue Lu
Since the treatment of glioma in clinic has been hindered by the blood-brain barrier (BBB) and blood-brain tumor barrier (BBTB), multifunctional glioma-targeted drug delivery systems that can circumvent both barriers have received increasing scrutiny. Despite recent research efforts have been made to develop multifunctional glioma-targeted liposomes by decorating two or more ligands, few successful trials have been achieved due to the limitation of ligand density on the surface of liposomes. In this study, we designed a Y-shaped multifunctional targeting material c(RGDyK)-pHA-PEG-DSPE, in which cyclic RGD (c(RGDyK)) and p-hydroxybenzoic acid (pHA) were linked with a short spacer...
April 5, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28387154/production-of-polyhydroxyalkanoates-pha-using-sludge-from-different-wastewater-treatment-processes-and-the-potential-for-medical-and-pharmaceutical-applications
#8
Wai Lam, Yujie Wang, Pui Ling Chan, Shun Wan Chan, Yiu Fai Tsang, Hong Chua, Peter Hoi Fu Yu
In this study, seven strains of bacteria with polyhydroxyalkanoates (PHA)-producing ability (i.e. Bacillus cereus, Pseudomonas putida, Bacillus pumilus, Pseudomona huttiensis, Yersinia frederiksenii, Aeromonas ichthiosmia, and Sphingopyxis terrae) were isolated from various waste treatment plants in Hong Kong. Simultaneous wastewater treatment and PHA accumulation were successfully achieved in the bioreactors using isolated bacteria from different sludges. At the organic loading less than 13,000 ppm, more than 95% of chemical oxygen demand (COD) was removed by the isolated strains before the decrease of PHA accumulation...
April 20, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28385589/lymphocyte-responses-of-rats-vaccinated-with-cdna-encoding-a-phosphoglycerate-kinase-of-fasciola-hepatica-fhpgk-and-f-hepatica-infection
#9
Agnieszka Wesołowska, Anna Zawistowska-Deniziak, Luke J Norbury, Przemysław Wilkowski, Anna M Pyziel, Wojciech Zygner, Halina Wędrychowicz
Lymphocyte responses in the blood, peritoneal fluid and both mesenteric and hepatic lymph nodes of cDNA-FhPGK/pCMV vaccinated and/or Fasciola hepatica infected rats of both sexes were investigated to provide an insight into the immune responses that develop in different body compartments. The immune response that developed in cDNA-FhPGK/pCMV vaccinated females contributed to partial protection against F. hepatica infection (54% reduction in fluke recovery), while more liver flukes were found in the livers and bile ducts of cDNA-FhPGK/pCMV vaccinated male rats than in unvaccinated animals (increase of 13%)...
April 3, 2017: Parasitology International
https://www.readbyqxmd.com/read/28385486/analysis-and-optimization-of-process-parameters-for-production-of-polyhydroxyalkanoates-along-with-wastewater-treatment-by-serratia-sp-istvkr1
#10
Asmita Gupta, Madan Kumar, Indu Shekhar Thakur
A previously reported biodegrading bacterial strain Serratia sp. ISTVKR1 was studied for polyhydroxyalkanoate (PHA) production along with wastewater contaminant removal. Nile red fluorescence, GC-MS, FT-IR, NMR and TEM confirmed the accumulation of homopolymer poly-3-hydroxyvalerate (PHV) within the bacterial cells. Analysis of culture after 72h of bacterial treatment showed maximum COD removal (8.4-fold), non-detection of organic contaminants such as 1H-Cyclopropa [a] naphthalene (R.T.=10.12) using GC-MS and increased proportion of elements like Cr, Mn, Fe, Ni, Cu, Cd and Pb in the bacterial cell pellets by SEM-EDX analysis...
March 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28383792/polyxydroxybutyrate-and-polyhydroxydodecanoate-produced-by-burkholderia-contaminans-ipt553-phb-and-phdd-produced-by-burkholderia
#11
Fernanda Matias, Carlos Alberto Brandt, Elda Sabino da Silva, Maria Filomena de Andrade Rodrigues
In this paper, we introduce a new Burkholderia contaminans capable of producing a newly characterized polymer METHODS AND RESULTS: CG-MS and Magnetic Nuclear Resonance (1) H and (13) C were used to determine the constitution of polymers produced on glucose, glucose with casein, sucrose, and sucrose with casein. Three pairs of primers were used to find the PHA synthase class and sequence. The synthesized polymers were composed by short chain length-PHA (scl-PHA), especially polyhydroxybutyrate (PHB), and medium chain length-PHAs (mcl-PHA), especially polyhydroxydodecanoate (PHDd) and their concentration, constitution, and molecular weight depend on carbon source used...
April 6, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28383134/in-vitro-t-lymphocyte-proliferation-by-carboxyfluorescein-diacetate-succinimidyl-ester-method-is-helpful-in-diagnosing-and-managing-primary-immunodeficiencies
#12
Elif Azarsiz, Neslihan Karaca, Birgul Ergun, Mehmet Durmuscan, Necil Kutukculer, Guzide Aksu
BACKGROUND: Functional studies besides routine laboratory tests for the definitive diagnosis of T lymphocyte disorders with isolated T or combined T/B-cell immunodeficiencies are important. We hereby summarized our experience with a carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay for the assessment of mitogenic T-cell proliferation responses in primary immunodeficiency (PID) patients who have not been diagnosed yet or genetically analyzed, but classified as probably having T-cell defects...
April 6, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28383031/serum-glycopatterns-as-novel-potential-biomarkers-for-diagnosis-of-acute-on-chronic-hepatitis-b-liver-failure
#13
Yaogang Zhong, Yonghong Guo, Xiawei Liu, Jiaxu Zhang, Tianran Ma, Jian Shu, Jiajun Yang, Jing Zhang, Zhansheng Jia, Zheng Li
Acute-on-chronic hepatitis B liver failure (ACHBLF) is an increasingly recognized distinct disease entity encompassing an acute deterioration of liver function in patients with cirrhosis, so little is known about the alterations of protein glycopatterns in serum with its development. We aimed to identify the alterations of serum glycopatterns in ACHBLF and probe the possibility of them as novel potential biomarkers for diagnosis of ACHBLF. As a result, there were 18 lectins (e.g., WFA, GSL-II, and PNA) to give significantly alterations of serum glycopatterns in ACHBLF compared with healthy controls (HC) (all p ≤ 0...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28381582/effect-of-temperature-and-food-restriction-on-immune-function-in-striped-hamsters-cricetulus-barabensis
#14
De-Li Xu, Xiao-Kai Hu, Yu-Fen Tian
Small mammals in the temperate area face seasonal fluctuations of temperature and food availability, both of which may influence their immune responses being critical to the survival. In the present study, we tested the hypothesis that low temperature and food restriction would suppress immune function in striped hamsters (Cricetulus barabensis). Thirty-seven adult male hamsters were randomly assigned into the warm (23±1°C) and the cold (5±1°C) groups, which were further divided into the fed and the food restricted groups, respectively...
April 5, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28381263/engineering-halomonas-species-td01-for-enhanced-polyhydroxyalkanoates-synthesis-via-crispri
#15
Wei Tao, Li Lv, Guo-Qiang Chen
BACKGROUND: Clustered regularly interspaced short palindromic repeats interference (CRISPRi) has provided an efficient approach for targeted gene inhibition. A non-model microorganism Halomonas species TD01 has been developed as a promising industrial producer of polyhydroxyalkanoates (PHA), a family of biodegradable polyesters accumulated by bacteria as a carbon and energy reserve compound. A controllable gene repression system, such as CRISPRi, is needed for Halomonas sp. TD01 to regulate its gene expression levels...
April 6, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28380407/bioelectrical-impedance-analysis-values-as-markers-to-predict-severity-in-critically-ill-patients
#16
Yeon Hee Lee, Jung-Dong Lee, Dae Ryong Kang, Jeong Hong, Jae-Myeong Lee
PURPOSE: We investigated bioelectrical impedance analysis (BIA)-derived parameters in critically ill patients to evaluate any differences between survivors and nonsurvivors. METHODS: We calculated severity scores for 241 critically ill surgical patients (161 male and 80 female; mean age, 62.9years) using three severity scoring systems (Acute Physiology and Chronic Health Evaluation II, Sequential Organ Failure Assessment, and Simplified Acute Physiology Score III)...
March 22, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/28370518/cost-effectiveness-of-diagnostic-testing-strategies-including-cervical-length-measurement-and-fibronectin-testing-in-women-with-symptoms-of-preterm-labor
#17
Gert-Jan van Baaren, Jolande Y Vis, Femke F Wilms, Martijn A Oudijk, Anneke Kwee, Martina M Porath, Hubertina Cj Scheepers, Marc Ea Spaanderman, Kitty Wm Bloemenkamp, Monique C Haak, Caroline J Bax, Jérôme Mj Cornette, Johannes J Duvekot, Bas Wa Nij Bijvanck, Jim van Eyck, Maureen Tm Franssen, Krystyna M Sollie, Frank Pha Vandenbussche, Mallory Woiski, Antoinette C Bolte, Joris Am van der Post, Patrick Mm Bossuyt, Brent C Opmeer, Ben Wj Mol
OBJECTIVE: To evaluate the cost-effectiveness of combining cervical length (CL) measurement and fetal fibronectin (fFN) tests for women with symptoms of preterm labor between 24 and 34 weeks gestation. METHODS: We performed a model-based cost-effectiveness analysis to evaluate seven test-treatment strategies based on CL and/or fFN in women with symptoms of preterm labor from a societal perspective, in which we weighted neonatal outcome and costs. Estimates of disease prevalence, test accuracy and costs, were based on two recently performed nationwide cohort studies in The Netherlands...
March 31, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28370210/the-effect-of-three-monthly-albendazole-treatment-on-th2-responses-differential-effects-on-ige-and-il-5
#18
Karin de Ruiter, Dicky L Tahapary, Linda Wammes, Aprilianto E Wiria, Firdaus Hamid, Lisette van Lieshout, Johannes W A Smit, Jeanine J Houwing-Duistermaat, Erliyani Sartono, Taniawati Supali, Maria Yazdanbakhsh
Helminth parasites induce a strong Th2 response, characterized by high levels of IgE and elevated signature cytokines such as IL-5. As many global deworming programs are underway, there is concern that this might lead to emergence of Th1-mediated pathologies when the counterbalancing helminth-induced Th2 response is absent. Therefore, we assessed the effect of deworming on Th2 mediated responses in a household-clustered randomised controlled trial in Indonesia. Total plasma IgE and whole-blood IL-5 responses to mitogen phytohaemagglutinin (PHA) were measured in 1494 and 682 subjects respectively, at baseline, 9 and 21 months after three-monthly single dose treatment with albendazole or placebo...
March 31, 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28366645/t-cell-activation-and-proliferation-following-acute-exercise-in-human-subjects-is-altered-by-storage-conditions-and-mitogen-selection
#19
Jacob A Siedlik, Jake A Deckert, Stephen H Benedict, Anuja Bhatta, Amanda J Dunbar, John P Vardiman, Philip M Gallagher
Recent work investigating exercise induced changes in immunocompetence suggests that some of the ambiguity in the literature is resultant from different cell isolation protocols and mitogen selection. To understand this effect, we compared post-exercise measures of T cell activation and proliferation using two different stimulation methods (costimulation through CD28 or stimulation with phytohaemagglutinin [PHA]). Further, we investigated whether exercise induced changes are maintained when T cell isolation from whole blood is delayed overnight in either a room temperature or chilled (4°C) environment...
March 30, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28365067/the-utility-of-a-phase-angle-analysis-in-patients-with-severe-motor-and-intellectual-disabilities
#20
Motomu Yoshida, Kimio Asagiri, Suguru Fukahori, Yoshiaki Tanaka, Naoki Hashizume, Shinji Ishii, Nobuyuki Saikusa, Naruki Higashidate, Daisuke Masui, Naoko Komatsuzaki, Hirotomo Nakahara, Minoru Yagi, Yushiro Yamashita
PURPOSE: The purpose of the present study was to evaluate whether evaluating the phase angle (PhA), in a bioelectrical impedance analysis (BIA) is useful for estimating the nutritional status of severe motor and intellectual disabilities (SMID) patients. SUBJECTS AND METHODS: This retrospective study included 31 SMID patients (mean age: 33.9±13.5years, median age: 29years (range: 18-58years), male/female: 23/8). First, each of the parameters from the total study population and the male and female SMID patients were compared with those of healthy Asian subjects...
March 29, 2017: Brain & Development
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