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https://www.readbyqxmd.com/read/28641986/novel-spectrophotometric-technique-for-rapid-determination-of-extractable-pha-using-sudan-black-dye
#1
Mauricio A Porras, Marcelo A Villar, María A Cubitto
Classical techniques employed to determine the amount of extractable poly(hydroxyalkanoate)s (PHAs) from cells, are laborious and destructive. Sudan black staining is commonly used in the laboratory to investigate the presence of intracellular PHA. The aim of the present study was to develop a low-cost alternative technique to achieve a quick determination of extractable intracellular PHA. This methodology employs a basic laboratory spectroscopy equipment and Sudan black dye for spectra determination. The correlation between the content of PHA in cell samples taken directly from the culture flask and its spectra was determined using partial least square regression analysis and simple linear regression analysis...
June 19, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28635367/in-vivo-and-in-vitro-depolymerizations-of-intracellular-medium-chain-length-poly-3-hydroxyalkanoates-produced-by-pseudomonas-putida-bet001
#2
Siti Nor Syairah Anis, Mohamad Suffian Mohamad Annuar, Khanom Simarani
In vivo and in vitro depolymerizations of intracellular medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) in Pseudomonas putida Bet001 grown on lauric acid was studied. Both processes were studied under optimum conditions for mcl-PHA depolymerization viz. 0.2 M Tris-HCl buffer, pH 9, ionic strength (I) = 0.2 M at 30 °C. For in vitro depolymerization studies, cell-free system was obtained from lysing bacterial cells suspension by ultrasonication at optimum conditions (frequency 37 kHz, 30% of power output, <25 (°)C for 120 minutes)...
June 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28634319/common-hepatic-artery-quadrifurcation-associated-with-right-hepatic-artery-from-superior-mesenteric-artery-during-laparoscopic-total-splenopancreasectomy
#3
Francesco Caruso, Giorgio Alessandri, Francesca Ciccarese, Giovanni Cesana, Matteo Uccelli, Giorgio Castello, Roberta Villa, Stefano Olmi
Hepatobiliopancreatic surgery is challenging becouse of the complexity of resections and reconstructions and variability of vascular anatomy. The arterial vascularization of the liver is susteined by the common hepatic artery (CHA) that originates from the celiac trunk (CT). The CHA bifurcates into the gastroduodenal artery (GDA) and proper hepatic artery (PHA) 0.5-1 cm medial to the common bile duct (CBD), and the PHA bifurcates into a right and a left branch at hepatic hilum. The most frequent variants are the right hepatic artery (RHA) from the superior mesenteric artery (SMA), the left hepatic artery (LHA) from the left gastric artery (LGA) or a combination of these two variants...
June 12, 2017: Annali Italiani di Chirurgia
https://www.readbyqxmd.com/read/28631225/definition-of-the-upper-reference-limit-for-thyroglobulin-antibodies-according-to-the-national-academy-of-clinical-biochemistry-guidelines-comparison-of-eleven-different-automated-methods
#4
F D'Aurizio, P Metus, A Ferrari, B Caruso, R Castello, D Villalta, A Steffan, K Gaspardo, F Pesente, N Bizzaro, E Tonutti, S Valverde, C Cosma, M Plebani, R Tozzoli
PURPOSE: In the last two decades, thyroglobulin autoantibodies (TgAb) measurement has progressively switched from marker of thyroid autoimmunity to test associated with thyroglobulin (Tg) to verify the presence or absence of TgAb interference in the follow-up of patients with differentiated thyroid cancer. Of note, TgAb measurement is cumbersome: despite standardization against the International Reference Preparation MRC 65/93, several studies demonstrated high inter-method variability and wide variation in limits of detection and in reference intervals...
December 2017: Auto- Immunity Highlights
https://www.readbyqxmd.com/read/28630728/parameters-for-assessment-of-the-inferior-acetabulum-morphology-in-300-adult-hips
#5
Munif A Hatem, Luiz A M da Cunha, João C M Abdo, Hal David Martin
The inferior acetabulum (IA) has been studied as a stabilizer of the hip in flexed positions with potential implications in femoroacetabular impingement and hip instability. However, there is a paucity of studies considering the normal morphology and parameters for assessment of the IA. The purpose of this study was to define parameters to assess the IA morphology and their normal range. Specifically, the objectives were to assess: (i) the width of the anterior horn (AH) and posterior horn (PH) of the acetabulum; (ii) the inclination of the articular surface of the AH angle (AHA) and PH angle (PHA) in the axial plane; (iii) the anterior opening angle of the IA and differences between genders...
January 2017: Journal of Hip Preservation Surgery
https://www.readbyqxmd.com/read/28629970/variation-analysis-of-bacterial-polyhydroxyalkanoates-production-using-saturated-and-unsaturated-hydrocarbons
#6
Saiqa Tufail, Sajida Munir, Nazia Jamil
Polyhydroxyalkanoates (PHA) are efficient, renewable and environment friendly polymeric esters. These polymers are synthesized by a variety of microbes under stress conditions. This study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. Six bacterial strains were isolated and identified as Bacillus cereus (KF270349), Klebsiella pneumoniae (KF270350), Bacillus subtilis (KF270351), Brevibacterium halotolerance (KF270352), Pseudomonas aeruginosa (KF270353), and Stenotrophomonas rhizoposid (KF270354) by ribotyping...
May 29, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629097/emerging-bone-tissue-engineering-via-polyhydroxyalkanoate-pha-based-scaffolds
#7
REVIEW
Janice Lim, Mingliang You, Jian Li, Zibiao Li
Polyhydroxyalkanoates (PHAs) are a class of biodegradable polymers derived from microorganisms. On top of their biodegradability and biocompatibility, different PHA types can contribute to varying mechanical and chemical properties. This has led to increasing attention to the use of PHAs in numerous biomedical applications over the past few decades. Bone tissue engineering refers to the regeneration of new bone through providing mechanical support while inducing cell growth on the PHA scaffolds having a porous structure for tissue regeneration...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28628985/enhanced-polyhydroxyalkanoate-production-by-mixed-microbial-culture-with-extended-cultivation-strategy
#8
Long Huang, Zhiqiang Chen, Qinxue Wen, Duu-Jong Lee
Low biomass output is a crucial reason for low polyhydroxyalkanoate (PHA) production in mixed microbial cultures (MMCs) PHA process. In this research, an extended cultivation strategy was proposed to rapidly expand the biomass yield of PHA accumulating MMCs and conserve the PHA accumulating ability simultaneously. High PHA content of the cultivated MMCs of 71.4% and 66.7% (higher than 62.1% of the seed biomass) in batch assays and biomass magnification of 43 and 52 were obtained after 10days of extended cultivation with and without sludge discharge, respectively...
June 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28620688/controlling-microbial-phb-synthesis-via-crispri
#9
Dan Li, Li Lv, Jin-Chun Chen, Guo-Qiang Chen
Microbial polyhydroxyalkanoates (PHA) are a family of biopolyesters with properties similar to petroleum plastics such as polyethylene (PE) or polypropylene (PP). Polyhydroxybutyrate (PHB) is the most common PHA known so far. Clustered regularly interspaced short palindromic repeats interference (CRISPRi), a technology recently developed to control gene expression levels in eukaryotic and prokaryotic genomes, was employed to regulate PHB synthase activity influencing PHB synthesis. Recombinant Escherichia coli harboring an operon of three PHB synthesis genes phaCAB cloned from Ralstonia eutropha, was transformed with various single guided RNA (sgRNA with its guide sequence of 20-23 bases) able to bind to various locations of the PHB synthase PhaC, respectively...
June 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28619635/production-and-characterization-of-polyhydroxyalkanoic-acid-from-bacillus-thuringiensis-using-different-carbon-substrates
#10
Olubusola A Odeniyi, Olakunle J Adeola
Polyhydroxyalkanoic acids (PHA) are biodegradable microbially synthesised polymers which can act as alternatives to conventional petrochemical based plastics. Bacterial isolates from soil and sewage samples were screened for the ability to accumulate PHA using Sudan Black B dye, and growth conditions of the best PHA accumulating bacterium was optimized. The PHA produced was characterized by FTIR spectroscopy. Eight PHA-producing bacteria were isolated and isolate SBC4 which had the highest percentage accumulation of 30% was genotypically identified as Bacillus thuringiensis and selected for further studies...
June 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28619066/hepatocyte-growth-factor-produced-in-lung-fibroblasts-enhances-non-small-cell-lung-cancer-cell-survival-and-tumor-progression
#11
Nobuhiro Kanaji, Masanao Yokohira, Yuko Nakano-Narusawa, Naoki Watanabe, Katsumi Imaida, Norimitsu Kadowaki, Shuji Bandoh
BACKGROUND: The influence of lung fibroblasts on lung cancer progression is not fully understood. METHODS: Lung fibroblasts (HFL1, MRC5, and IMR90 cells) and non-small cell lung cancer (NSCLC)-derived cell lines (A549, EBC1, and HI1017) were cultured under serum-free conditions, and the resulting culture media were designated "cell-conditioned media". Cell survival (viability) was assessed by WST-1 assay. Concentrations of hepatocyte growth factor (HGF) were measured by ELISA...
June 15, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28601124/impact-of-short-term-nutritional-supplementation-on-surrogate-markers-of-undernutrition-in-hemodialysis-patients-prospective-real-life-interventional-study
#12
Andreja Ocepek, Sebastjan Bevc, Robert Ekart
INTRODUCTION: Hemodialysis (HD) patients are at increased risk for undernutrition, especially protein wasting. We present the results of a prospective study in HD patients after 4 months of intervention with oral nutritional supplements (ONS). METHODS: After a 3-month wash-out period, 92 HD patients were enrolled in the study. Patients were tested for undernutrition with composite parameters, laboratory tests, bioelectrical impedance analysis (BIA), and hand-grip strength test (HGS)...
June 9, 2017: Clinical Nephrology
https://www.readbyqxmd.com/read/28595804/techno-economic-feasibility-of-waste-biorefinery-using-slaughtering-waste-streams-as-starting-material-for-biopolyester-production
#13
Khurram Shahzad, Michael Narodoslawsky, Muhammad Sagir, Nadeem Ali, Shahid Ali, Muhammad Imtiaz Rashid, Iqbal Mohammad Ibrahim Ismail, Martin Koller
The utilization of industrial waste streams as input materials for bio-mediated production processes constitutes a current R&D objective not only to reduce process costs at the input side but in parallel, to minimize hazardous environmental emissions. In this context, the EU-funded project ANIMPOL elaborated a process for the production of polyhydroxyalkanoate (PHA) biopolymers starting from diverse waste streams of the animal processing industry. This article provides a detailed economic analysis of PHA production from this waste biorefinery concept, encompassing the utilization of low-quality biodiesel, offal material and meat and bone meal (MBM)...
June 5, 2017: Waste Management
https://www.readbyqxmd.com/read/28594941/low-level-laser-lll-attenuate-lps-induced-inflammatory-responses-in-mesenchymal-stem-cells-via-the-suppression-of-nf-%C3%AE%C2%BAb-signaling-pathway-in-vitro
#14
Kan Yin, Rongjia Zhu, Shihua Wang, Robert Chunhua Zhao
BACKGROUND: Considering promising results in animal models and patients, therapeutic use of MSCs for immune disease is likely to undergo continued evaluation. Low-lever laser (LLL) has been widely applied to retard the inflammatory reaction. LLL treatment can potentially be applied in anti-inflammatory therapy followed by stem cell therapy. AIM OF THE STUDY: The purpose of this study was to investigate the effect of LLL (660 nm) on the inflammatory reaction induced by LPS in human adipose derived mesenchymal stem cells (hADSCs) and pertinent mechanism...
2017: PloS One
https://www.readbyqxmd.com/read/28593901/pseudohypoaldosteronism-types-i-and-ii-little-more-than-a-name-in-common
#15
Dídac Casas-Alba, Jordi Vila Cots, Laura Monfort Carretero, Loreto Martorell Sampol, Maria-Christina Zennaro, Xavier Jeunemaitre, Juan Antonio Camacho Díaz
Pseudohypoaldosteronism (PHA) comprises a diverse group of rare diseases characterized by sodium and potassium imbalances incorrectly attributed to a defect in aldosterone production. Two different forms of PHA have been described, type I (PHAI) and type II (PHAII). PHAI has been subclassified into renal and systemic. Given the rarity and heterogeneity of this group of disorders we report three patients who carry PHA and a brief revision of current literature focused on the comparative analysis of PHAI and PHAII...
May 1, 2017: Journal of Pediatric Endocrinology & Metabolism: JPEM
https://www.readbyqxmd.com/read/28593540/mesorhizobium-bacterial-strains-isolated-from-the-legume-lotus-corniculatus-are-an-alternative-source-for-the-production-of-polyhydroxyalkanoates-phas-to-obtain-bioplastics
#16
Marta Marcos-García, Paula García-Fraile, Alena Filipová, Esther Menéndez, Pedro F Mateos, Encarna Velázquez, Tomáš Cajthaml, Raúl Rivas
Polyhydroxyalkanoic acids (PHAs) are natural polyesters that can be used to produce bioplastics which are biodegradable. Numerous microorganisms accumulate PHAs as energy reserves. Combinations of different PHAs monomers lead to the production of bioplastics with very different properties. In the present work, we show the capability of strains belonging to various phylogenetic lineages within the genus Mesorhizobium, isolated from Lotus corniculatus nodules, to produce different PHA monomers. Among our strains, we found the production of 3-hydroxybutyrate, 3-hydroxyvalerate, 3-hydroxydodecanoate, and 3-hydroxyhexadecanoate...
June 7, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28591726/multifunctional-targeted-liposomal-drug-delivery-for-efficient-glioblastoma-treatment
#17
Zakia Belhadj, Changyou Zhan, Man Ying, Xiaoli Wei, Cao Xie, Zhiqiang Yan, Weiyue Lu
Glioblastoma multiforme (GBM) has been considered to be the most malignant brain tumors. Due to the existence of various barriers including the blood-brain barrier (BBB) and blood-brain tumor barrier (BBTB) greatly hinder the accumulation and deep penetration of chemotherapeutics, the treatment of glioma remains to be the most challenging task in clinic. In order to circumvent these hurdles, we developed a multifunctional liposomal glioma-targeted drug delivery system (c(RGDyK)/pHA-LS) modified with cyclic RGD (c(RGDyK)) and p-hydroxybenzoic acid (pHA) in which c(RGDyK) could target integrin αvβ3 overexpressed on the BBTB and glioma cells and pHA could target dopamine receptors on the BBB...
May 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28591645/fast-method-for-the-determination-of-short-chain-length-polyhydroxyalkanoates-scl-phas-in-bacterial-samples-by-in-vial-thermolysis-ivt
#18
F Abbondanzi, G Biscaro, G Carvalho, L Favaro, P Lemos, M Paglione, C Samorì, C Torri
A new method based on the GC-MS analysis of thermolysis products obtained by treating bacterial samples at a high temperature (above 270°C) has been developed. This method, here named "In-Vial-Thermolysis" (IVT), allowed for the simultaneous determination of short-chain-length polyhydroxyalkanoates (scl-PHA) content and composition. The method was applied to both single strains and microbial mixed cultures (MMC) fed with different carbon sources. The IVT procedure provided similar analytical performances compared to previous Py-GC-MS and Py-GC-FID methods, suggesting a similar application for PHA quantitation in bacterial cells...
June 4, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28591561/over-expression-of-groesl-in-cupriavidus-necator-for-heterotrophic-and-autotrophic-isopropanol-production
#19
Jillian Marc, Estelle Grousseau, Eric Lombard, Anthony J Sinskey, Nathalie Gorret, Stéphane E Guillouet
We previously reported a metabolic engineering strategy to develop an isopropanol producing strain of Cupriavidus necator leading to production of 3.4gL(-1) isopropanol. In order to reach higher titers, isopropanol toxicity to the cells has to be considered. A toxic effect of isopropanol on the growth of C. necator has been indeed observed above a critical value of 15gL(-1). GroESL chaperones were first searched and identified in the genome of C. necator. Native groEL and groES genes from C. necator were over-expressed in a strain deleted for PHA synthesis...
June 4, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28587886/microbial-selection-strategies-for-polyhydroxyalkanoates-production-from-crude-glycerol-effect-of-olr-and-cycle-length
#20
André Freches, Paulo C Lemos
Crude glycerol from biodiesel manufacture can be used as carbon source for microbial fermentations. The production of polyhydroxyalkanoates by manipulating the Sequencing Batch Reactor (SBR) selection stage of microbial mixed cultures (MMC) using high organic loading rates (OLR, 50CmM/day) and different cycles lengths (6, 12 and 24h) was optimized. Batch-production of polyhydroxybutyrate (PHB) presented an accumulation capacity in the high range (0.44g/g) after 3 pulses of 50CmM, with a final content of 59% PHB/wt...
June 3, 2017: New Biotechnology
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