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Ovarian transplantation

Tracy L Habermehl, Kate C Parkinson, Gene B Hubbard, Yuji Ikeno, Jennifer I Engelmeyer, Björn Schumacher, Jeffrey B Mason
Cardiovascular disease, rare in premenopausal women, increases sharply at menopause and is typically accompanied by chronic inflammation. Previous work in our laboratory demonstrated that replacing senescent ovaries in post-reproductive mice with young, actively cycling ovaries restored many health benefits, including decreased cardiomyopathy and restoration of immune function. Our objective here was to determine if depletion of germ cells from young transplanted ovaries would alter the ovarian-dependent extension of life and health span...
December 13, 2018: GeroScience
Zahra Kalhori, Mehri Azadbakht, Malek Soleimani Mehranjani, Mohammad Ali Shariatzadeh
BACKGROUND: Many studies have reported that inflammation and oxidative stress are involved in the pathogenesis of polycystic ovary syndrome (PCOS). Bone marrow mesenchymal stromal cells (BM-MSCs) have anti-oxidant and anti-inflammation properties. In this study, we investigate the beneficial effect of stem cell therapy on folliculogenesis in mice with induced PCOS METHODS: Mouse model of PCOS was performed through daily injection of testosterone enanthate (1 mg/100 g/body weight subcutaneous (s...
December 2018: Cytotherapy
André Tichelli, Eric Beohou, Myriam Labopin, Gérard Socié, Alicia Rovó, Manuela Badoglio, Anja van Biezen, Peter Bader, Rafael F Duarte, Grzegorz Basak, Nina Salooja
Importance: Incidence and risk factors of second solid cancers (SSCs) that occur after hematopoietic stem cell transplantation (HSCT) are well documented. However, clinical outcome data of patients who developed an SSC after HSCT are limited. Objective: To assess the outcome of patients with an SSC occurring after HSCT from the time of SSC diagnosis. Design, Setting, and Participants: This cohort study used data of 4065 patients from 26 countries registered with the European Society for Blood and Marrow Transplantation, which has maintained clinical data since 1977 of patients who received a transplant...
November 21, 2018: JAMA Oncology
Ibrahim El-Serafi, Orlaith Loy, Ying Zhao, Sandra Oerther, Jonas Mattsson
INTRODUCTION: Treosulfan is an alkylating agent that is used for the treatment of ovarian cancer and for conditioning prior to stem cell transplantation. It is a prodrug that is activated non-enzymatically to two active epoxides. OBJECTIVES: To optimize a protocol for both in vivo samples handling and in vitro drug preparation. Treosulfan stability was tested in biological fluids at different conditions as well as for its cytotoxicity on cell lines. RESULTS: Plasma samples can be safely frozen for a short period up to 8h, however; for longer periods, samples should be acidified...
November 26, 2018: Pharmaceutical Development and Technology
Luciana Lamarão Damous, Ana Elisa Teófilo Saturi de Carvalho, Juliana Sanajotti Nakamuta, Marcos Eiji Shiroma, Andressa Cristina Sposato Louzada, José Maria Soares-Jr, José Eduardo Krieger, Edmund C Baracat
The use of secretome may be a new strand of cell therapy, which is equal to or even superior to the injection of live cells, called cell-free therapy. In ovarian transplantation, this approach may be a therapeutic possibility for the ovarian graft in hypoxia. We designed the present study to evaluate whether the cell-free therapy with the secretome of adipose tissue-derived stem cells (ASCs) in rat frozen-thawed ovarian grafts could protect a graft against ischemic injury. A single dose of rat ASCs secretome or vehicle was injected into the bilateral frozen-thawed ovaries of 18 adult female rats immediately after an autologous transplant...
November 21, 2018: Stem Cell Research & Therapy
A B Brito, Dcc Brito, W B Silva, Apr Rodrigues, J R Figueiredo, Sfs Domingues, R R Santos
SummaryOvarian biopsies from five health adult monkeys were collected by exploratory laparotomy. Preantral follicles (primordial, primary, and secondary) were classified as normal or degenerated and submitted to morphometric analysis in which granulosa cell counts and the areas of follicles, oocytes, and oocyte nuclei were measured. Ovarian fragments were also immunolabelled for the quantitative analysis of VEGFA and CD31 protein expression in the ovarian tissue and in the preantral follicles. In total, 213 preantral follicles was examined for morphometry and morphological classification...
November 19, 2018: Zygote: the Biology of Gametes and Early Embryos
Wookyeom Yang, Gwan Hee Han, Ha-Yeon Shin, Eun-Ju Lee, Hanbyoul Cho, Doo Byung Chay, Jae-Hoon Kim
PURPOSE: Modulated electro-hyperthermia (mEHT), known as oncothermia, is an anticancer therapy that induces radiofrequency thermal damage to the cancer tissues. This study aimed to evaluate the potential effectiveness of mEHT as a therapeutic tool in ovarian and cervical cancer. MATERIALS AND METHODS: We used both tumor-bearing mice and ovarian and cervical OVCAR-3, SK-OV-3, HeLa and SNU-17 cancer cell lines to investigate the effects of mEHT in vivo and in vitro, respectively, and determine whether it was enhanced by cotreatment with an autophagy inhibitor...
November 14, 2018: International Journal of Hyperthermia
Upendra Kumar Soni, Sangappa Basanna Chadchan, Vijay Kumar, Vaibhave Ubba, Mohammad Tariq Ali Khan, Budai Shanmukha Vivek Vinod, Rituraj Konwar, Himangsu Kousik Bora, Srikanth Kumar Rath, Sharad Sharma, Rajesh Kumar Jha
Endometriosis is a prevalent gynecological disorder that eventually gives rise to painful invasive lesions. Increased levels of transforming growth factor-beta1 (TGF-B1) have been reported in endometriosis. However, details of the effects of high TGF-B1 on downstream signaling in ectopic endometrial tissue remain obscure. We induced endometriotic lesions in mice by surgical auto-transplantation of endometrial tissues to the peritoneal regions. We then treated endometriotic (ectopic and eutopic endometrial tissues) and non-endometriotic (only eutopic endometrial tissues) animal groups with either active TGF-B1 or PBS...
November 13, 2018: Biology of Reproduction
Dávid Á Kovács, Andries E Braat, Volkert A L Huurman, Andrzej G Baranski
Simultaneous pancreas-kidney transplant is the ultimate therapy for patients who have uncontrolled and complicated type 1 diabetes mellitus with end-stage renal disease. The combined pancreas transplant provides a euglycemic milieu for the kidney and protects it from recurrence of diabetic complications. Our patient, a 41-year-old woman with end-stage diabetic nephropathy and history of multiple abdominal surgeries (ovarian cyst fenestration, adnexal extirpation, abdominal wall reconstruction), including urinary diversion (Bricker loop, above double J stent), underwent simultaneous pancreas-kidney transplant...
November 6, 2018: Experimental and Clinical Transplantation
Stine Aagaard Lunding, Susanne Elisabeth Pors, Stine Gry Kristensen, Claus Yding Andersen, Janni Vikkelsø Jeppesen, Kirsten Tryde Macklon, Anders Nyboe Andersen, Anette Tønnes Pedersen
OBJECTIVE: To describe and demonstrate a simple and secure procedure for laparoscopic autotransplantation of fragmented ovarian cortical tissue in women with diminished ovarian reserve as part of in vitro activation (IVA) of ovarian follicles. DESIGN: Step-by-step video explanation of the surgical procedure with still pictures and surgical video clips to demonstrate the detailed technique. SETTING: Fertility clinic and obstetrics and gynecology department at a university hospital...
November 2018: Fertility and Sterility
Maïté Fransolet, Laure Noël, Laurie Henry, Soraya Labied, Silvia Blacher, Michelle Nisolle, Carine Munaut
PURPOSE: To evaluate the efficiency of ovarian tissue treatment with Z-VAD-FMK, a broad-spectrum caspase inhibitor, to prevent follicle loss induced by ischemia/reperfusion injury after transplantation. METHODS: In vitro, granulosa cells were exposed to hypoxic conditions, reproducing early ischemia after ovarian tissue transplantation, and treated with Z-VAD-FMK (50 μM). In vivo, cryopreserved human ovarian fragments (n = 39) were embedded in a collagen matrix containing or not Z-VAD-FMK (50 μM) and xenotransplanted on SCID mice ovaries for 3 days or 3 weeks...
November 3, 2018: Journal of Assisted Reproduction and Genetics
Daniel Lantsberg, Adel Farhi, Inna Zaslavsky-Paltiel, Barbara G Silverman, Liat Lerner-Geva, Raoul Orvieto
OBJECTIVE: To evaluate the delivery rate and to characterize patients following ovarian tissue cryopreservation (OTCP) who did not undergo auto-transplantation. METHODS AND MATERIALS: All consecutive cancer patients admitted to our IVF unit, from January 2004 to December 2015, who underwent OTCP for the purpose of fertility preservation without autotransplantation were analyzed. The cohort included 338 patients and was linked to the National Live Birth registry of the Israel Ministry of Health in order to determine whether the women delivered following the cancer diagnosis...
October 29, 2018: Journal of Assisted Reproduction and Genetics
Maryam Saber, Hussein Eimani, Malek Soleimani Mehranjani, Abdolhossein Shahverdi, Hamid Reza Momeni, Rouhollah Fathi, Somayeh Tavana
One of the challenges that must be overcome during ovarian tissue transplantation is Ischaemia/Reperfusion Injury (IRI). The most important hypothesis explaining the cellular events in I/R processes are calcium overload and oxygen free radicals constitute. Here, we study the effect of verapamil on IRI, and consequently on follicle survival during ovarian transplants in an autograft model. Female mice were randomly assigned into three groups in order to ovarian autotransplantation as follow: Group 1 (Control group), Group 2 (Transplanted group) and Groups 3 (Transplanted + Verapamil group)...
December 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Haidong Li, Qichao Yao, Feng Xu, Ning Xu, Wen Sun, Saran Long, Jianjun Du, Jiangli Fan, Jingyun Wang, Xiaojun Peng
For the precision resection, development of near-infrared (NIR) fluorescent probe based on specificity identification tumor-associated enzyme for lighting-up the tumor area, is urgent in the field of diagnosis and treatment. Overexpression of γ-glutamyltranspeptidase, one of the cell-membrane enzymes, known as a biomarker is concerned with the growth and progression of ovarian, liver, colon and breast cancer compared to normal tissue. In this work, a remarkable enzyme-activated NIR fluorescent probe NIR-SN-GGT was proposed and synthesized including two moieties: a NIR dicyanoisophorone core as signal reporter unit; γ-glutamyl group as the specificity identification site...
2018: Frontiers in Chemistry
Antoni Llueca, Dolors Piquer, Yasmine Maazouzi, Carmen Medina, Katty Delgado, Anna Serra, Javier Escrig
INTRODUCTION: Epithelioid hemangioendothelioma is a malignant mesenchymal tumor of unknown etiology. They tend to be asymptomatic or with non-specific symptoms. The lesion is usually multiple and variable size. PRESENTATION OF CASE: We describe a clinical case of a 23-years-old patient diagnosed with a pelvic mass, a possible uterine fibroid or adnexal mass, and multiple liver lesions that seemed an advanced ovarian cancer presentation and after liver biopsy turned out to be a hepatic epithelioid hemangioendothelioma...
October 12, 2018: International Journal of Surgery Case Reports
Elham Shojafar, Malek Soleimani Mehranjani, Seyed Mohammad Ali Shariatzadeh
BACKGROUND: Ovarian tissue autografting is a fertility restoration technique that is frequently used in young women with cancer who undergo radio/chemotherapy. A limiting factor in this technique is ischemia-reperfusion (I/R) damage. Because adipose-derived mesenchymal stromal cells (ADMSCs) protect different ischemic tissues against I/R damage, we examined the effect of ADMSC transplantation at the graft site in mice ovary autografting. METHOD: Mice were divided into three groups: control, autograft and autograft + ADMSCs...
October 22, 2018: Cytotherapy
Ya D Karpova, Yu V Lyupina, N M Alabedal'karim, E I Legach, G A Bozhok, N P Sharova
Implantation of ovarian tissue allografts in outbred Wistar rats and inbred August rats against the background of induction of donor-specific tolerance was accompanied by an increase in liver content of mononuclear cells expressing LMP2 immune of proteasome subunit by day 37 after transplantation in comparison with day 0. Graft rejection, on the contrary, was associated with a decrease in the number of LMP2+ cells in the liver of rats of both lines during this period. The difference in the content of these cells and the graft take rate were higher in Wistar rats...
October 2018: Bulletin of Experimental Biology and Medicine
Tae-Hee Kim, Jong Ho Choi, Yesl Jun, Seung Mook Lim, Sohae Park, Jin-Young Paek, Sang-Hoon Lee, Ji-Young Hwang, Gi Jin Kim
Placenta-derived mesenchymal stem cells (PD-MSCs) have numerous advantages over other adult MSCs that make them an attractive cell source for regenerative medicine. Here, we demonstrate the therapeutic effect of PD-MSCs in ovariectomized (Ovx) rats and compare their efficacy when generated via a conventional monolayer culture system (2D, naïve) and a spheroid culture system (3D, spheroid). PD-MSC transplantation significantly increased the estradiol level in Ovx rats compared with the non-transplantation (NTx) group...
October 17, 2018: Scientific Reports
Swati Agarwal, Faisal A Alzahrani, Asif Ahmed
BACKGROUND: Throughout history, menopause has been regarded as a transition in a woman's life. With the increase in life expectancy, women now spend more than a third of their lives in menopause. During these years, women may experience intolerable symptoms both physically and mentally, leading them to seek clinical advice. It is imperative for healthcare providers to improve the quality of life by reducing bothersome menopausal symptoms and preventing disorders such as osteoporosis and atherosclerosis...
October 14, 2018: International Journal of Molecular Sciences
C-H Lin, S-N Chang, C-H Chen
OBJECTIVE: Hormonal changes, in either men or women experiencing chronic kidney disease, cause decreased libido, erectile dysfunction, dysmenorrhea, and ovarian dysfunction, all of which may contribute to infertility. Patients' endocrinal and sexual function could be rapidly restored after successful renal transplantation (RTx), which may assist in achieving a successful pregnancy. However, fetuses will be exposed to the immunosuppressive agents passed on from the female recipients, which may be another potential factor leading to a poor fetal outcome...
October 2018: Transplantation Proceedings
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