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https://www.readbyqxmd.com/read/30121885/mir146a-rs2910164-g-c-polymorphism-is-associated-with-incidence-of-preeclampsia-in-gestational-diabetes-patients
#1
Dina M Abo-Elmatty, Eman T Mehanna
Preeclampsia and gestational diabetes are common pregnancy disorders that may be interrelated. MIR146A rs2910164 (G/C) is a functional polymorphism that was associated with several diseases. This study aimed to investigate the frequency of rs2910164 polymorphism and its possible correlation with the incidence of preeclampsia in gestational diabetes patients. The study involved 250 pregnant women divided into 80 healthy control subjects, 85 gestational diabetes patients only, and 85 patients of gestational diabetes combined with preeclampsia...
August 18, 2018: Biochemical Genetics
https://www.readbyqxmd.com/read/30121832/resonance-light-scattering-aptasensor-for-urinary-8-hydroxy-2-deoxyguanosine-based-on-magnetic-nanoparticles-a-preliminary-study-of-oxidative-stress-association-with-air-pollution
#2
Lixia Tao, Qiaoli Yue, Yining Hou, Yongping Wang, Chunying Chen, Chen-Zhong Li
An aptamer based method is described for the determination of 8-hydroxy-2'-deoxyguanosine (8-OHdG) using resonance light scattering (RLS). Magnetic nanoparticles (MNPs) were employed as RLS probes. The probe DNA was placed on the surface of MNPs, which produces a rather low RLS signal. If, however, probe DNA hybridizes with the aptamer against 8-OHdG, a sandwich structure will be formed. This results in a significant enhancement of RLS intensity. The aptamer was used as the recognition element to capture 8-OHdG...
August 18, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/30121803/presence-of-anaerobic-bacteria-in-the-urinary-tract-of-catheterized-icu-patients
#3
Keren Lee Rozenfeld, Orna Nitzan, Avi Peretz
The purpose of our study was to examine the extent of anaerobic bacteriuria in catheterized patients in the intensive care unit (ICU) and to search for risk factors for anaerobic bacteriuria. A urine culture was collected from each patient every 2 days during their ICU stay and incubated under aerobic and anaerobic conditions. Aerobic and anaerobic blood cultures were collected as well. Demographic, clinical, and laboratory data were collected from patient files. Ninety patients were included in this study, 32 women (35...
August 18, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/30121704/purple-urine-bag-syndrome
#4
Willian Eduardo Ito, Sílvio Henrique Maia de Almeida
No abstract text is available yet for this article.
August 18, 2018: International Urogynecology Journal
https://www.readbyqxmd.com/read/30121694/diagnostic-value-comparison-of-urothelium-carcinoma-among-urine-exfoliated-cells-fluorescent-in-situ-hybridization-fish-examination-computerized-tomography-ct-scan-and-urine-cytologic-examination
#5
Tao Yang, Yan Li, Jing Li, Junjiang Liu, Xinna Deng, Gang Wang
BACKGROUND This study aimed to compare the clinical effectiveness of urine exfoliated cells FISH examination, CT scan, and urine cytologic examination on the diagnosis of upper urinary tract urothelium carcinoma with hematuresis symptom. MATERIAL AND METHODS A total of 30 patients with suspicious upper urinary tract urothelium carcinoma between Aug 2010 and Aug 2011 were enrolled, including 23 males and 7 females. All the subjects received urine exfoliated cells FISH examination, CT scan, and urine cytologic examination...
August 19, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/30121654/urine-cofilin-1-detection-for-predicting-type-1-cardiorenal-syndrome-in-the-coronary-care-unit-a-gold-nanoparticle-and-laser-based-approach
#6
Hsing-Yu Chen, Chien Chou, Chih-Hsiang Chang, Nien-Gen Lee, Pei-Chun Yu, Yu-Chi Chen, Heng-Chih Pan, Pei-Chun Fan, Chih-Wei Yang, Wen-Jin Cherng, Yung-Chang Chen
BACKGROUND: Type 1 cardiorenal syndrome (CRS) is a severe complication for acute decompensated heart failure patients. This study aimed at evaluating the feasibility of using the gold nanoparticle-based localized surface plasmon-coupled fluorescence biosensor (LSPCFB) to detect urine cofilin-1 as a biomarker for predicting CRS among patients in the coronary care unit (CCU). METHODS: A total of 44 patients were included with prospectively collected urine and blood samples...
August 17, 2018: Cardiorenal Medicine
https://www.readbyqxmd.com/read/30121207/impaired-cognitive-processing-speed-in-type-1-diabetic-patients-who-had-severe-recurrent-hypoglycaemia
#7
Stefano Bortolotti, Lisa Zarantonello, Ambra Uliana, Nicola Vitturi, Sami Schiff, Patrizia Bisiacchi, Angelo Avogaro, Piero Amodio, Alberto Maran
AIMS: To detect whether adults with type 1 diabetes mellitus (T1DM) have lower cognitive performance than healthy individuals and to detect risk factors for low cognitive performance. METHODS: Twenty-six adults with T1DM and twenty-six healthy subjects matched for age, gender and educational level were compared for cognitive performance by a chronometric computerized test measuring visuo-spatial working memory (N-Back) and by two validated neuropsychological tests (Mini Mental State Examination, Animal Naming Test)...
August 10, 2018: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/30121168/liquid-biopsy-biomarkers-of-renal-interstitial-fibrosis-based-on-urinary-exosome
#8
Lv Chun-Yan, Zhao Zi-Yi, Yang Tian-Lin, Wang Yi-Li, Li Bao, Lv Jiao, Ding Wei-Jun
Renal interstitial fibrosis (RIF) is difficult to diagnose. This paper explored liquid biopsy markers in urinary exosomes derived from RIF patients. Urine samples from 32 patients with various degrees of RIF and 20 non-RIF patients were collected. The size and morphology of urinary exosomes isolated by polyethylene glycol were observed with electron microscopy. Protein biomakers of exosomes were analyzed by Western blot. qRT-PCR was used to detect the levels of biomarkers (miR-29c, miR-21, E-cadherin, and vimentin) of epithelial mesenchymal transition in urinary exosomes...
August 16, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/30121037/efficient-cephalexin-degradation-using-active-chlorine-produced-on-ruthenium-and-iridium-oxide-anodes-role-of-bath-composition-analysis-of-degradation-pathways-and-degradation-extent
#9
Lic A Perea, Ricardo E Palma-Goyes, Jorge Vazquez-Arenas, Issis Romero-Ibarra, Carlos Ostos, Ricardo A Torres-Palma
The elimination of cephalexin (CPX) using electro-generated Cl2 -active on Ti/RuO2 -IrO2 anode was assessed in different effluents: deionized water (DW), municipal wastewater (MWW) and urine. Single Ti/RuO2 and Ti/IrO2 catalysts were prepared to compare their morphologies and electrochemical behavior against the binary DSA. XRD and profile refinement suggest that Ti/RuO2 -IrO2 forms a solid solution, where RuO2 and IrO2 growths are oriented by the TiO2 substrate through substitution of Ir by Ru atoms within its rutile-type structure...
August 11, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/30120931/kinetic-time-courses-of-lambda-cyhalothrin-metabolites-after-dermal-application-of-matador-ec-120-in-volunteers
#10
Rania Khemiri, Jonathan Côté, Hamadi Fetoui, Michèle Bouchard
A controlled kinetic study was conducted in volunteers dermally exposed to the widely used lambda-cyhalothrin pyrethroid pesticide to document the time courses of relevant biomarkers of exposure, in order to better assess biomonitoring data in workers. Matador® EC120 formulation (120 g/l) was applied on 40 cm2 of the forearm at a 0.25 mg/kg dose of lambda-cyhalothrin and left without occlusion or washing for 6 h. The application site was then washed thoroughly with soap and water. The kinetic time courses of cis-3-(2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropane carboxylic acid (CFMP) and 3-phenoxybenzoic acid (3-PBA) metabolites were determined in plasma and urine up to 84 h post-application...
August 15, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/30120867/diagnostic-protocols-for-the-management-of-pregnancy-of-unknown-location
#11
J A Ross
'Pregnancy of unknown location' is a non-diagnostic transvaginal ultrasound scan in early pregnancy. The scan may be non- diagnostic because the pregnancy is too small to identify, too difficult to identify or gone - already passed during the process of miscarriage. Many patients are surprised to learn that urine pregnancy tests are more sensitive in detecting a pregnancy than ultrasound scans, but as long as this remains the case, or until we discover a biomarker that can locate a pregnancy, the management of women with PUL will continue to form a significant part of the workload of Early Pregnancy Units...
August 18, 2018: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/30120856/use-of-vhh-antibodies-for-development-of-antigen-detection-test-for-visceral-leishmaniasis
#12
Claudia Abeijon, Julia Dilo, Jacqueline M Tremblay, Agostinho G Viana, Lilian L Bueno, Silvio F G Carvalho, Ricardo T Fujiwara, Charles B Shoemaker, Antonio Campos-Neto
We have recently developed a sensitive and specific urine-based antigen detection ELISA for the diagnosis of visceral leishmaniasis (VL). This assay used rabbit IgG and chicken IgY polyclonal antibodies specific for the Leishmania infantum proteins iron superoxide dismutase 1 (Li-isd1), tryparedoxin1 (Li-txn1), and nuclear transport factor 2 (Li-ntf2). However, polyclonal antibodies have limitations for upscaling and continuous supply. To circumvent these hurdles, we began to develop immortalized monoclonal antibodies...
August 18, 2018: Parasite Immunology
https://www.readbyqxmd.com/read/30120332/expression-profiling-in-a-mammalian-host-reveals-the-strong-induction-of-genes-encoding-lysm-domain-containing-proteins-in-enterococcus-faecium
#13
Margherita Cacaci, Caroline Giraud, Loic Leger, Riccardo Torelli, Cecilia Martini, Brunella Posteraro, Valentina Palmieri, Maurizio Sanguinetti, Francesca Bugli, Axel Hartke
Enterococcus faecium is an important health care-associated pathogen that is difficult to treat due to the high level of antibiotic resistance of clinical isolates. The identification of new potential therapeutic targets or vaccination strategies is therefore urgently needed. In this regard, we carried out a transcriptomic analysis of the E. faecium vancomycin-resistant strain AUS0004, comparing the gene expression of bacteria grown under laboratory conditions and bacteria isolated from an infection site. This analysis highlighted more than 360 genes potentially induced under infection conditions...
August 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30120273/porous-sno-2-nanoparticles-based-ion-chromatographic-determination-of-non-fluorescent-antibiotic-chloramphenicol-in-complex-samples
#14
Nadeem Muhammad, Abdul Rahman, Muhammad Adnan Younis, Qamar Subhani, Khurram Shehzad, Hairong Cui, Yan Zhu
Nowadays, there are rising concerns about the extensive use of the antibiotics such as chloramphenicol (CAP), has threatened the human life in the form of various vicious diseases. The limited selectivity and sensitivity of confirmatory techniques (UV and electrochemical) and non-fluorescence property of CAP make its determination a challenging task in the modern pharmaceutical analysis. In order to redeem the selective, sensitive and cost-effective fluorescence methodology, here by the dual role of synthesized porous SnO2 nanoparticles were exploited; (i) a porous sorbent in a µ-QuEChERS based sample preparation and as (ii) a stimulant for the transformation of non-fluorescent analytes namely CAP and p-nitrophenol (p-NP) into their respective fluorescent product...
August 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30120219/double-trouble-severe-hypernatremia-secondary-to-central-diabetes-insipidus-complicated-by-hypercalcemic-nephrogenic-diabetes-insipidus-a-case-report
#15
Muhammad Abdullah Zain, Abbas Raza, Muhammad Owais Hanif, Zehra Tauqir, Maryam Khan, Muhammad J Mahboob, Fariha Ashraf, Waqas Javed Siddiqui, Hasan Arif, Larry E Krevolin
BACKGROUND Patients with malignancies often have electrolyte abnormalities. We present a case of a patient with central diabetes insipidus secondary to metastatic pituitary invasion complicated by hypercalcemic nephrogenic diabetes insipidus. CASE REPORT We present a case of 40-year-old female with a history of stage IV breast cancer with skeletal and leptomeningeal metastasis, who was admitted with polyuria, polydipsia, and recent onset of confusion. The patient was found to have profound hypernatremia and severe hypercalcemia with normal parathyroid and vitamin D serum levels...
August 18, 2018: American Journal of Case Reports
https://www.readbyqxmd.com/read/30120082/tissue-specific-effects-of-targeted-mutation-of-mir29b1-in-rats
#16
Hong Xue, Guangyuan Zhang, Aron M Geurts, Kristie Usa, David M Jensen, Yong Liu, Michael E Widlansky, Mingyu Liang
BACKGROUND: miR-29 is a master regulator of extracellular matrix genes, but conflicting data on its anti-fibrotic effect have been reported. miR-29 improves nitric oxide (NO) production in arterioles by targeting Lypla1. Mir29b1 targeted mutation exacerbates hypertension in a model derived from the Dahl salt-sensitive rat. We examined the effect of Mir29b1 mutation on tissue fibrosis and NO levels with a focus on kidney regions. METHODS: Mir29b1 targeted mutant rats on the genetic background of SS-Chr13BN rats were studied...
August 14, 2018: EBioMedicine
https://www.readbyqxmd.com/read/30119986/pyelonephritis-treatment-in-the-community-emergency-department-cephalosporins-vs-first-line-agents
#17
Shaylyn Vogler, Emily Pavich
OBJECTIVE: The purpose of this study was to assess treatment with a fluoroquinolone or trimethoprim-sulfamethoxazole versus cephalosporins for pyelonephritis in discharged patients from a community hospital setting. METHODS: A retrospective chart review was completed for adult female patients who received a prescription for a cephalosporin, fluoroquinolone or trimethoprim-sulfamethoxazole for the treatment of pyelonephritis within the network of a large healthcare system...
August 8, 2018: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/30119978/cardiometabolic-risk-indicators-for-kidney-disease-in-mexican-patients-with-type-2-diabetes
#18
Lubia Velázquez-López, Rubí Hernández-Sánchez, Ivonne Roy García, Abril Violeta Muñoz-Torres, Patricia Medina-Bravo, Jorge Escobedo-de la Peña
INTRODUCTION: Kidney disease is one of the main complications in the diabetic patient. Risk factors such as obesity, dyslipidemia, and hyperglycemia have been associated with increased urinary albumin excretion (UAE) and decreased glomerular filtration rates (GFR). However, the data are inconsistent. The objective of this study was to identify the primary risk factors associated with kidney disease in Mexican patients with type 2 diabetes. METHODS: A cross-sectional study was done in 395 patients with type 2 diabetes from four primary-care clinics...
August 14, 2018: Archives of Medical Research
https://www.readbyqxmd.com/read/30119972/combination-of-dispersive-solid-phase-extraction-and-deep-eutectic-solvent-based-air-assisted-liquid-liquid-microextraction-followed-by-gas-chromatography-mass-spectrometry-as-an-efficient-analytical-method-for-the-quantification-of-some-tricyclic-antidepressant
#19
Ali Mohebbi, Saeid Yaripour, Mir Ali Farajzadeh, Mohammad Reza Afshar Mogaddam
A dispersive solid phase extraction coupled with deep eutectic solvent-based air-assisted liquid-liquid microextraction has been developed and applied to the extraction and preconcentration of some tricyclic antidepressant drugs in the human urine and plasma samples prior to their determination by gas chromatography-mass spectrometry. In this method, a sorbent (C18 ) is first added into an alkaline aqueous sample and dispersed by vortexing. By this action, the analytes are adsorbed onto the sorbent. Then, the sorbent particles are isolated from the aqueous solution by centrifugation...
August 10, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/30119970/l-arginine-attenuates-blood-pressure-and-reverses-the-suppression-of-angiogenic-risk-factors-in-a-rat-model-of-preeclampsia
#20
G O Oludare, H D Jinadu, O O Aro
This study investigated the effect of L-arginine on blood pressure, angiogenic factors and liver enzymes in pregnant rats administered L-NAME. Thirty-six female Sprague-Dawley rats weighing between 150-170 g were divided into 4 groups of the control (normal saline), L-NAME (50 mg/kg b.w. intraperitoneal injection from days 13-18 of pregnancy), L-NAME + L-arginine (50 mg/kg b.w. and 1 g/kg b.w of L-arginine from days 13 to 18) and L-arginine (1 g/kg b.w administered orally from days 13 to 18). Urine samples were collected on day 18 of pregnancy and the animals were sacrificed on day 19 of pregnancy for blood pressure parameters, angiogenic and liver enzyme assays...
August 7, 2018: Pathophysiology: the Official Journal of the International Society for Pathophysiology
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