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Aminoglycoside resistance

Gamaliel López-Leal, Fernanda Cornejo-Granados, Juan Manuel Hurtado-Ramírez, Alfredo Mendoza-Vargas, Adrian Ochoa-Leyva
Microbiome sequencing has become the standard procedure in the study of new ecological and human-constructed niches. To our knowledge, this is the first report of a metagenome from the water of a greenhouse drain. We found that the greenhouse is not a diverse niche, mainly dominated by Rhizobiales and Rodobacterales. The analysis of the functions encoded in the metagenome showed enrichment of characteristic features of soil and root-associated bacteria such as ABC-transporters and hydrolase enzymes. Additionally, we found antibiotic resistances genes principally for spectinomycin, tetracycline, and aminoglycosides...
2018: Standards in Genomic Sciences
Iman Dandachi, Selma Chabou, Ziad Daoud, Jean-Marc Rolain
In recent years, extended ESBL and carbapenemase producing Gram negative bacteria have become widespread in hospitals, community settings and the environment. This has been triggered by the few therapeutic options left when infections with these multi-drug resistant organisms occur. The emergence of resistance to colistin, the last therapeutic option against carbapenem-resistant bacteria, worsened the situation. Recently, animals were regarded as potent antimicrobial reservoir and a possible source of infection to humans...
2018: Frontiers in Microbiology
Cecilia Aristimuño Ficoseco, Flavia I Mansilla, Natalia C Maldonado, Hortencia Miranda, María E Fátima Nader-Macias, Graciela M Vignolo
In order to eliminate the widespread use of antibiotics in livestock production, the research for alternatives has increased lately. This study examined the safety of 40 lactic acid bacteria (LAB) isolated from bovine feedlot environment and previously selected as potential probiotics. A high sensitivity prevalence to ampicillin (AMP, 100%), gentamicin (GEN, 96.3%), kanamycin (KAN, 96.3%), clindamycin (CLI, 85.2%), chloramphenicol (CHL, 92.6%) and streptomycin (STR, 88.9%) while moderate and high resistance against erythromycin (ERY, 48%) and tetracycline (TET, 79%) respectively, were determined...
2018: Frontiers in Microbiology
Kiran Gordhanbhai Piparva, Gaurav Jansari, Anil P Singh
Background: The emergence of drug-resistant mycobacteria becomes a significant public health problem globally creating an obstacle to effective tuberculosis (TB) control. Gujarat, Maharashtra, and Andhra Pradesh DRS survey estimated that the proportion of multidrug-resistant TB (MDR-TB) is 2.1% (in new TB cases) and 15% (in previously treated cases). Programmatic management of MDR-TB implemented under Revised National Tuberculosis Control Programme in India in 2007. Objectives: The objective of this study is to evaluate treatment outcome and adverse drug reactions (ADRs) of category IV...
October 2018: Perspectives in Clinical Research
Hasnain Javed, Zofia Bakuła, Małgorzata Pleń, Hafiza Jawairia Hashmi, Zarfishan Tahir, Nazia Jamil, Tomasz Jagielski
Pakistan ranks 5th among the world's highest tuberculosis (TB) burden countries alongside the 6th among countries with the highest burden of drug-resistant TB, including multi-drug resistant (MDR)-TB. Methods for rapid and reliable drug susceptibility testing (DST) are prerequisite for the prompt institution of effective anti-TB treatment. The aim of this study was to evaluate the efficiency of Genotype MTBDR plus and MTBDR sl assays for the detection of MDR and (pre-) extensively drug-resistant (XDR-TB) isolates in Pakistan...
2018: Frontiers in Microbiology
Yusha Lei, Jiayu Zhang, Lijia Cao, Renxin Zhao, Jie Feng, Bing Li
OBJECTIVES: The draft genome sequence of Xenophilus sp. E41, a multiple antibiotics resistant strain isolated from activated sludge reactors treating wastewater with high cephalexin concentration, was reported in the present study. METHODS: Genomic DNA of Xenophilus sp. E41 was extracted and sequenced using the Illumina NovaSeq 6000 system. The generated sequence reads were assembled using MEGAHIT in combination with SOAPdenovo. Mauve and CompareM were used to align Xenophilus sp...
October 11, 2018: Journal of Global Antimicrobial Resistance
Gaspar Banfalvi
The in vitro susceptibility of gentamicin fractions against Fusarium growth was the subject of this retrospective study. Fusariosis was earlier an exceptionally rare human disease and an unrealistic idea to treat soil saprophytes and plant pathogens with expensive antibiotics such as gentamicins or their minor components. Disseminated fusariosis is now the second most frequent lethal fungal infection after aspergillosis especially in neutropenic patients with hematologic malignancy. Results of this study obtained between May and November 1973 were interesting but not practicable and remained unpublished...
October 12, 2018: Applied Microbiology and Biotechnology
Konstantinos Vazouras, Romain Basmaci, Julia Bielicki, Laura Folgori, Theoklis Zaoutis, Mike Sharland, Yingfen Hsia
PURPOSE: Urinary tract infections (UTIs) are common bacterial infections among children. OBJECTIVE: To systematically review the antimicrobials used for febrile UTIs in paediatric clinical trials and meta-analyse the observed cure rates and reasons for treatment failure. MATERIALS AND METHODS: We searched Medline, Embase and Cochrane central databases between January 1, 1990, and November 24, 2016, combining MeSH and free-text terms for: "urinary tract infections", AND "therapeutics", AND "clinical trials" in children (age range 0-18 years)...
October 11, 2018: Drugs
Tae Hee Lee, Joo-Hee Hwang, Woo Kon Lee, Min-Kyoung Shin, Hye Ryun Woo, Kyung Min Chung, Chang-Seop Lee
Pathogenic gram-negatives that produce 16S ribosomal RNA methyltransferases (16S RMTases) have already been distributed all over the world. To investigate the predominance of aminoglycoside resistance associated with 16S RMTases in Korea, we collected a total of 222 amikacin resistant Gram-negative clinical isolates from patient specimens between 1999 and 2015 from three hospital banks across Korea. ArmA and rmtB were the predominant 16S RMTase genes responsible for aminoglycoside-resistant isolates circulating in Korean community settings although only one rmtA -producing isolate was detected in 2006...
October 15, 2018: Journal of Korean Medical Science
Kaidi Telling, Mailis Laht, Age Brauer, Maido Remm, Veljo Kisand, Matti Maimets, Tanel Tenson, Irja Lutsar
BACKGROUND: We aimed to identify the main spreading clones, describe the resistance mechanisms associated with carbapenem- and/or multidrug-resistant P. aeruginosa and characterize patients at risk of acquiring these strains in Estonian hospitals. METHODS: Ninety-two non-duplicated carbapenem- and/or multidrug-resistant P. aeruginosa strains were collected between 27th March 2012 and 30th April 2013. Clinical data of the patients was obtained retrospectively from the medical charts...
October 11, 2018: BMC Infectious Diseases
Jun Yang, Zao-Hai Zeng, Man-Jun Yang, Zhi-Xue Cheng, Xuan-Xian Peng, Hui Li
The development of antibiotic resistance in Vibrio alginolyticus represents a threat to human health and fish farming. Environmental NaCl regulation of bacterial physiology is well documented, but whether the regulation contributes to antibiotic resistance remains unknown. To explore this, we compared minimum inhibitory concentration (MIC) of V. alginolyticus cultured in different media with 0.5% to 10% NaCl, and found that the MIC increased as the NaCl concentration increased, especially for aminoglycoside antibiotics...
October 11, 2018: Environmental Microbiology
Kavita Raja, Molly Antony, S Harikrishnan
Introduction: Infective endocarditis (IE) is an infection of the heart valves with an aggregation of bacteria in a fibrin plaque called vegetation. Aims and Objectives: This is a retrospective study of all infective endocarditis cases due to alpha haemolytic streptococci and enterococci. Methods: All cases of infective endocarditis cases due to alpha haemolytic streptococci and enterococci in a period of three years from 1st January 2010 to 31st December 2012 were included...
October 2018: Indian Journal of Pathology & Microbiology
Mario Tumbarello, Angela Raffaella Losito, Giamarellou Helen
PURPOSE OF REVIEW: In the absence of randomized clinical trial data, questions remain regarding the optimal treatment of carbapenem-resistant Enterobacteriaceae (CRE) infections. CRE have historically been susceptible to polymyxins, tigecycline or aminoglycosides (mostly gentamicin), and these antibiotics have long been considered the drugs of choice for CRE infections, although varying rates of resistance to all have been reported. This review looks at data from clinical studies assessing the outcomes of CRE infections treated with different antibiotic regimens...
October 5, 2018: Current Opinion in Infectious Diseases
Birgit Wolters, Eva Fornefeld, Sven Jechalke, Jian-Qiang Su, Yong-Guan Zhu, Søren J Sørensen, Kornelia Smalla, Samuel Jacquiod
Municipal sewage sludge (MSS) is often directly applied to fields despite concerns regarding the spread of harmful microbes and associated resistance genes (RGs). In this four month microcosm study, the dynamics of prokaryotic communities in agricultural soil and changes in mobile genetic elements (MGEs) and RGs following amendment with stabilized MSS was investigated. TaqMan-based quantitative real-time (q)PCR showed that RG prevalence was high when compared to untreated soil and genes for class 1 integrons (intI1), streptomycin RGs (aadA, strA) and tetracycline RG (tet(W)), were detectable for the duration of the four month study...
October 5, 2018: FEMS Microbiology Ecology
Himen Salimizand, Abolfazl Raafati Zomorodi, Davood Mansury, Mostafa Khakshoor, Omid Azizi, Sepideh Khodaparast, Zohre Baseri, Pezhman Karami, Sajjad Zamanlou, Hadi Farsiani, Yousef Amini, Bagher Moradi, Zahra Meshkat, Hana Salimizand, Sepideh Hasanzadeh, Hamid Sadeghian
PURPOSE: Acinetobacter baumannii-calcoaceticus complex (ABC) make a great burden on health-care systems due to hospital-acquired infections and antibacterial resistance. Aminoglycoside in combination with other antibacterials used as treatment options. However, ABC species overcome this class of antibacterials in different ways. This study provides a comprehensive report on the distribution of aminoglycoside modifying enzymes (AMEs) and 16S rRNA methylase in Acinetobacter baumannii and Acinetobacter nosocomialis isolated from various provinces in Iran...
October 4, 2018: Infection, Genetics and Evolution
Michael Fruci, Keith Poole
Exposure of P. aeruginosa to the aminoglycoside (AG) paromomycin (PAR) induced expression of the PA3720-armR locus and the mexAB-oprM multidrug efflux operon that AmgR controls, although PAR induction of mexAB-oprM was independent of armR. Multiple AGs promoted mexAB-oprM expression and this was lost in the absence of the amgRS locus encoding an aminoglycoside-activated envelope stress-responsive 2-component system (TCS). Purified AmgR bound to the mexAB-oprM promoter region consistent with this response regulator directly regulating expression of the efflux operon...
2018: PloS One
Mutasim E Ibrahim
To determine the distribution and resistance profiles of Gram-negative bacteria (GNB) in intensive care units (ICUs) at King Abdullah Hospital in Bisha, Saudi Arabia. Methods: A record based retrospective study was conducted from December 2016 to January 2018. In total, 3736 non-duplicate clinical specimens from the general intensive care unit (ICU), neonatal ICU (NICU), and coronary CU (CCU) were analyzed for pathogens. Results: Of 3736 specimens, 9.6% (358) were positive for pathogens, and GNB constituted the majority (290/358; 81%)...
October 2018: Saudi Medical Journal
Louis Zimmermann, Julie Kempf, Florian Briée, Jitendriya Swain, Marie-Paule Mingeot-Leclercq, Jean-Luc Décout
Amphiphilic aminoglycosides (AAGs) constitute a new class of antibacterial compounds targeting the bacterial membranes. We have identified the 3',6-dinonyl neamine 9 as a broad spectrum antibacterial AAG. Here, we report on the synthesis, antibacterial activity and eukaryotic cytotoxicity of new 3',6-dialkyl neamines designed in order to finely delineate the structure-activity relationships relating their activity to a lipophilicity window. New broad-spectrum antibacterial derivatives were obtained carrying two identical linear or branched alkyl groups or two different linear alkyl groups...
September 5, 2018: European Journal of Medicinal Chemistry
Rolf Hirsch, Jochen Wiesner, Alexander Marker, Yvonne Pfeifer, Armin Bauer, Peter E Hammann, Andreas Vilcinskas
Background: The ability of MDR Gram-negative bacteria to evade even antibiotics of last resort is a severe global challenge. The development pipeline for conventional antibiotics cannot address this issue, but antimicrobial peptides (AMPs) offer an alternative solution. Objectives: Two insect-derived AMPs (LS-sarcotoxin and LS-stomoxyn) were profiled to assess their suitability for systemic application in humans. Methods: The peptides were tested against an extended panel of 114 clinical MDR Gram-negative bacterial isolates followed by time-kill analysis, interaction studies and assays to determine the likelihood of emerging resistance...
October 1, 2018: Journal of Antimicrobial Chemotherapy
Gregory H Tyson, Jonathan L Sabo, Maria Hoffmann, Chih-Hao Hsu, Sampa Mukherjee, Jacqueline Hernandez, Glenn Tillman, Jamie L Wasilenko, Jovita Haro, Mustafa Simmons, Wanda Wilson Egbe, Patricia L White, Uday Dessai, Patrick F Mcdermott
Objectives: To sequence the genomes and determine the genetic mechanisms for linezolid resistance identified in three strains of Enterococcus isolated from cattle and swine caecal contents as part of the US National Antimicrobial Resistance Monitoring System (NARMS) surveillance programme. Methods: Broth microdilution was used for in vitro antimicrobial susceptibility testing to assess linezolid resistance. Resistance mechanisms and plasmid types were identified from data generated by WGS on Illumina® and PacBio® platforms...
October 1, 2018: Journal of Antimicrobial Chemotherapy
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