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https://www.readbyqxmd.com/read/30118149/automation-of-the-in-vitro-micronucleus-assay-using-the-imagestream-%C3%A2-imaging-flow-cytometer
#1
Matthew A Rodrigues
The in vitro micronucleus (MN) assay is a well-established test for evaluating genotoxicity and cytotoxicity. The use of manual microscopy to perform the assay can be laborious and often suffers from user subjectivity and interscorer variability. Automated methods including slide-scanning microscopy and conventional flow cytometry have been developed to eliminate scorer bias and improve throughput. However, these methods possess several limitations such as lack of cytoplasmic visualization using slide-scanning microscopy and the inability to visually confirm the legitimacy of MN or storage of image data for re-evaluation using flow cytometry...
August 17, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/30116524/recruitment-of-sublingual-microcirculation-using-handheld-incident-dark-field-imaging-as-a-routine-measurement-tool-during-the-postoperative-de-escalation-phase-a-pilot-study-in-post-icu-cardiac-surgery-patients
#2
Zühre Uz, Can Ince, Philippe Guerci, Yasin Ince, Renata P Araujo, Bulent Ergin, Matthias P Hilty, Thomas M van Gulik, Bas A de Mol
Background: Management of tissue perfusion following cardiac surgery is a challenging task where common clinical parameters do not reflect microcirculatory dysfunction. Heterogeneity in blood flow perfusion and abnormalities in capillary density characterize microcirculatory dysfunction. The restoration of a normal microcirculation may become a novel target for therapy in the future in addition to macrocirculatory parameters. The aim of this study is to determine how the sublingual microcirculatory parameters vary at the bedside in post-cardiac surgery patients which underwent diuretic therapy to correct fluid overload...
2018: Perioperative Medicine
https://www.readbyqxmd.com/read/30112686/the-prognostic-value-of-simplified-eeg-in-out-of-hospital-cardiac-arrest-patients
#3
Ward Eertmans, Cornelia Genbrugge, Jolien Haesen, Carolien Drieskens, Jelle Demeestere, Margot Vander Laenen, Willem Boer, Dieter Mesotten, Jo Dens, Ludovic Ernon, Frank Jans, Cathy De Deyne
BACKGROUND: We previously validated simplified electroencephalogram (EEG) tracings obtained by a bispectral index (BIS) device against standard EEG. This retrospective study now investigated whether BIS EEG tracings can predict neurological outcome after cardiac arrest (CA). METHODS: Bilateral BIS monitoring (BIS VISTA™, Aspect Medical Systems, Inc. Norwood, USA) was started following intensive care unit admission. Six, 12, 18, 24, 36 and 48 h after targeted temperature management (TTM) at 33 °C was started, BIS EEG tracings were extracted and reviewed by two neurophysiologists for the presence of slow diffuse rhythm, burst suppression, cerebral inactivity and epileptic activity (defined as continuous, monomorphic, > 2 Hz generalized sharp activity or continuous, monomorphic, < 2 Hz generalized blunt activity)...
August 15, 2018: Neurocritical Care
https://www.readbyqxmd.com/read/30107082/the-parsortix%C3%A2-cell-separation-system-a-versatile-liquid-biopsy-platform
#4
M Craig Miller, Peggy S Robinson, Christopher Wagner, Daniel J O'Shannessy
Cancer cells from solid tumors can enter the circulatory system and survive to subsequently form distant metastases. The CellSearch® system (Menarini-Silicon Biosystems, Huntingdon Valley, PA) was the first, FDA-cleared system that provided a reliable tool for the investigation of circulating tumor cells (CTCs), which have been shown to be strongly associated with poor survival and therapy failure. Since that time, a number of new technologies have been introduced to improve CTC detection and/or isolation for further characterization...
August 14, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/30106518/critical-assessment-of-the-mdx-mouse-with-ex-vivo-eccentric-contraction-of-the-diaphragm-muscle
#5
Gregory C Addicks, Phillip Marshall, Bernard J Jasmin, Jean-Marc Renaud, Hongbo Zhang, Keir J Menzies
Muscle function and health progressively deteriorate during the progression of muscle dystrophies. The ability to objectively characterize muscle function and muscle damage is useful not only when comparing variants of dystrophy models, but also for characterizing the effects of interventions aiming to improve or halt the progressive decline of muscle function and muscle health. The protocols in this chapter describe the use of ex vivo eccentric contraction of the diaphragm muscle as a measure of muscle susceptibility to damage...
August 14, 2018: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/30106517/profiling-interactome-networks-with-the-halotag-nappa-in-situ-protein-array
#6
Junshi Yazaki, Mary Galli, Alice Y Kim, Joseph R Ecker
Physical interactions between proteins and other molecules can be evaluated at a proteome scale using protein arrays, a type of high-throughput pulldown assay. We developed a modified in situ protein array known as the nucleic acid programmable protein assay (NAPPA) that allows the screening of thousands of open reading frames (ORFs) at a lower cost, with less labor, and in less time than conventional protein arrays. The HaloTag-NAPPA protein array can efficiently capture proteins expressed in situ on a glass slide using the Halo high-affinity capture tag...
August 14, 2018: Current Protocols in Plant Biology
https://www.readbyqxmd.com/read/30106515/muscle-stem-cell-immunostaining
#7
Shuaiyu Wang, Bao Zhang, Gregory C Addicks, Hui Zhang, Keir J Menzies, Hongbo Zhang
Muscle stem cells (MuSCs) are essential for maintaining muscle homeostasis by providing progenitor cells for muscle regeneration after injury and in muscular diseases. MuSC properties dynamically change, reflecting physiology or pathological status. For instance, MuSCs are activated after muscle injury, but become exhausted in late stages of Duchenne Muscular Dystrophy (DMD) disease and senescent during aging. Therefore, characterization of MuSCs, including proliferation, activation, senescence, and apoptosis, etc...
August 14, 2018: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/30106511/computational-prediction-of-carbohydrate-binding-proteins-and-binding-sites
#8
Huiying Zhao, Ghazaleh Taherzadeh, Yaoqi Zhou, Yuedong Yang
Protein-carbohydrate interaction is essential for biological systems, and carbohydrate-binding proteins (CBPs) are important targets when designing antiviral and anticancer drugs. Due to the high cost and difficulty associated with experimental approaches, many computational methods have been developed as complementary approaches to predict CBPs or carbohydrate-binding sites. However, most of these computational methods are not publicly available. Here, we provide a comprehensive review of related studies and demonstrate our two recently developed bioinformatics methods...
August 14, 2018: Current Protocols in Protein Science
https://www.readbyqxmd.com/read/30105659/tailored-healthcare-two-perspectives-on-the-development-and-use-of-patient-profiles
#9
Tessa Dekkers, Dorijn F L Hertroijs
Calls for a more tailored approach to the management of cardiometabolic and musculoskeletal diseases have been increasing. Although tailored care is a centuries-old concept, it is still unclear how it should be best practised. The current paper introduces two phenotype-based Dutch approaches to support tailored care. One approach focuses on patients with type 2 diabetes, the other on patients undergoing total joint replacement. Using the patient profiling approach, both projects propose that care can be tailored by the assessment of biopsychosocial patient characteristics, stratification of patients into subgroups of patients with similar care needs, abilities, and preferences (so-called patient profiles) and tailoring of care in concordance with the common care preferences of these profiles...
August 13, 2018: Advances in Therapy
https://www.readbyqxmd.com/read/30102466/6-chloropurine-ribonucleosides-from-chloropyrimidines-one-pot-synthesis
#10
Renaud Zelli, Waël Zeinyeh, Jean-Luc Décout
A one-pot glycosylation and cyclization procedure is described for the synthesis of 6-chloropurine ribonucleosides from chloropyrimidines. From such a procedure and modification of the obtained chloropurine ribonucleosides, many drug candidates or molecular tools for biological study designed from their similarity to naturally occurring nucleosides could be obtained. The synthesis begins by preparation of several amidinoaminochloropyrimidines as precursors for the one-pot procedure. Then, by adding trimethylsilyl trifluoromethanesulfonate (TMSOTf) to a mixture of a pyrimidine and 1-O-acetyl-2,3,5-tri-O-benzoyl-β-D-ribose, different 6-chloropurine ribonucleosides are obtained...
August 13, 2018: Current Protocols in Nucleic Acid Chemistry
https://www.readbyqxmd.com/read/30102462/triggered-cell-cell-fusion-assay-for-cytoplasmic-and-organelle-intermixing-studies
#11
Daniel Feliciano, Jonathon Nixon-Abell, Jennifer Lippincott-Schwartz
Different multicellular organisms undergo cell-cell fusion to form functional syncytia that support specialized functions necessary for proper development and survival. For years, monitoring the structural consequences of this process using live-cell imaging has been challenging due to the unpredictable timing of cell fusion events in tissue systems. Here we present a triggered vesicular stomatitis virus G-protein (VSV-G)-mediated cell-cell fusion assay that can be used to synchronize fusion between cells. This allows the study of cellular changes that occur during cell fusion...
August 13, 2018: Current Protocols in Cell Biology
https://www.readbyqxmd.com/read/30102414/quantitative-accuracy-of-ct-numbers-in-ct-theoretical-analyses-and-experimental-studies
#12
Ran Zhang, Juan P Cruz-Bastida, Daniel Gomez-Cardona, John Hayes, Ke Li, Guang-Hong Chen
PURPOSE: The CT number accuracy, i.e., CT number bias, plays an important role in clinical diagnosis.When strategies to reduce radiation dose are discussed, it is important to make sure that the CT number bias is controlled within an acceptable range. The purpose of this paper was to investigate the dependence of CT number bias on radiation dose level and on image contrast (i.e., the difference in CT number between the ROI and the background) in Computed Tomography (CT). METHODS: A lesion-background model was introduced to theoretically study how the CT number bias changes with radiation exposure level and with CT number contrast when a simple linear reconstruction algorithm such as filtered backprojection (FBP) is used...
August 13, 2018: Medical Physics
https://www.readbyqxmd.com/read/30099659/accuracy-assessment-of-wireless-transponder-tracking-in-the-operating-room-environment
#13
Roeland Eppenga, Koert Kuhlmann, Theo Ruers, Jasper Nijkamp
PURPOSE: To evaluate the applicability of the Calypso® wireless transponder tracking system (Varian Medical Systems Inc., USA) for real-time tumor motion tracking during surgical procedures on tumors in non-rigid target areas. An accuracy assessment was performed for an extended electromagnetic field of view (FoV) of 27.5 × 27.5 × 22.5 cm (which included the standard FoV of 14 × 14 × 19 cm) in which 5DOF wireless Beacon® transponders can be tracked. METHODS: Using a custom-made measurement setup, we assessed single transponder relative accuracy, absolute accuracy and jitter throughout the extended FoV at 1440 locations interspaced with 2...
August 11, 2018: International Journal of Computer Assisted Radiology and Surgery
https://www.readbyqxmd.com/read/30099544/robotic-assisted-spine-surgery-using-the-mazor-xtm-system-2-dimensional-operative-video
#14
Lee A Tan, Ronald A Lehman
We demonstrate the setup and workflow of performing robotic spine surgery using the Mazor XTM system (MAZOR Robotics Inc, Orlando, Florida) in this video. An illustrative case was presented, including detailed steps for S2AI screw and lumbar pedicle screw placement using robotic assistance. A step-by-step narration is provided along with discussion of surgical nuances. Robotic spine surgery can be a safe and efficient method for screw placement, which can potentially reduce the risk of screw malposition. Spine surgeons should be familiar with this technology and keep this technique in their armamentarium...
August 7, 2018: Operative Neurosurgery (Hagerstown, Md.)
https://www.readbyqxmd.com/read/30096218/three-dimensional-printing-of-poly-glycerol-sebacate-fumarate-gadodiamide-poly-ethylene-glycol-diacrylate-structures-and-characterization-of-mechanical-properties-for-soft-tissue-applications
#15
Prashanth Ravi, Jamie Wright, Panos S Shiakolas, Tré R Welch
Bioresorbable materials have been frequently used to three-dimensional (3D) print biomedical structures. In this study, we developed a technique to 3D print poly(glycerol sebacate fumarate) gadodiamide (Rylar)-poly(ethylene glycol) diacrylate (PEGDA) samples and investigated their mechanical and thermal properties as a function of (PS) and ultraviolet intensity (UVI). The Young's modulus (E), ultimate tensile strength (UTS), failure strain (ɛF ), and glass transition temperature (Tg ) showed strong correlation with PS and UVI...
August 10, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/30096088/current-management-of-childhood-ptosis
#16
Daniel T Weaver
PURPOSE OF REVIEW: Blepharoptosis is a common problem encountered in the pediatric ophthalmology clinic. The presentation is obvious to both parents and referring physicians and often prompts urgent consultation. The current classification and management of childhood ptosis will be reviewed. RECENT FINDINGS: Recent refinements in techniques utilizing new materials hold promise for better, more predictable outcomes and improved long-term results. Autogenous tensor tendon fascia lata harvested from the patient's thigh remains the gold standard for many ptosis surgeons in frontalis suspension; however, other materials are commonly utilized, including silicone rod, Gore-Tex (ePTFE; W...
September 2018: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/30095230/the-feasibility-and-effectiveness-of-pass-plus-a-lay-health-worker-delivered-comprehensive-intervention-for-autism-spectrum-disorders-pilot-rct-in-a-rural-low-and-middle-income-country-setting
#17
Gauri Divan, Vivek Vajaratkar, Percy Cardozo, Shilpa Huzurbazar, Mamta Verma, Elizabeth Howarth, Richard Emsley, Carol Taylor, Vikram Patel, Jonathan Green
The treatment gap for autism globally is high. Our previous PASS intervention, delivered by community based lay health workers, showed effectiveness. This article reports the development and evaluation of a new "PASS 'Plus'" intervention in a rural population in India. Using formative research methods, we supplemented the PASS intervention with additional (Plus) modules to address autism comorbidities. This is the first time that a rigorous methodology has been used to evaluate autism symptom outcomes in a low and middle-income country setting...
August 10, 2018: Autism Research: Official Journal of the International Society for Autism Research
https://www.readbyqxmd.com/read/30095186/global-metabolomic-responses-in-urine-from-atm-deficient-mice-in-response-to-ld-50-30-gamma-irradiation-doses
#18
Evagelia C Laiakis, Tytus D Mak, Steven J Strawn, Yi-Wen Wang, Bo-Hyun Moon, Pelagie Ake, Albert J Fornace
Exposures to ionizing radiation (IR) may either be accidental or intentional, for medical purposes or even through terrorist actions. As certain populations emerge to be more radiosensitive than others, it is imperative to assess those individuals and treat them accordingly. To demonstrate the feasibility of rapid identification of such cases, we utilized the highly radiosensitive mouse model Atm-/- in the C57BL/6 background, and evaluated the urinary responses in 8-10 week old male mice at early time points (4, 24, and 72 h) after exposure to their respective LD50/30 doses [4 Gy for Atm-/- , and 8 Gy for wild type (WT)]...
August 10, 2018: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/30095173/efficacy-of-adjunctive-photodynamic-therapy-in-the-treatment-of-generalized-aggressive-periodontitis-a-randomized-controlled-clinical-trial
#19
Tugce Borekci, Suleyman Emre Meseli, Ulku Noyan, Bahar Eren Kuru, Leyla Kuru
OBJECTIVES: Generalized aggressive periodontitis (GAgP) is a distinct type of periodontal disease characterized by rapid loss of attachment and alveolar bone occurring in young individuals. Photodynamic therapy (PDT) was introduced in periodontology as an adjunctive approach to non-surgical periodontal treatment (NPT) in periodontitis patients. In this trial, the aim was to evaluate the clinical and microbiological effects of adjunctive PDT to NPT in patients with GAgP. METHODS: In this prospective controlled clinical study, 24 systemically healthy, non-smoking subjects with GAgP were enrolled...
August 10, 2018: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/30092774/an-early-analysis-of-cost-utility-of-baroreflex-activation-therapy-in-advanced-chronic-heart-failure-in-germany
#20
Oleg Borisenko, Jochen Müller-Ehmsen, JoAnn Lindenfeld, Erik Rafflenbeul, Christian Hamm
BACKGROUND: This study aimed to evaluate cost-utility of baroreflex activation therapy (BAT) using the Barostim neo™ device (CVRx Inc., Minneapolis, MN, USA) compared with optimized medical management in patients with advanced chronic heart failure (NYHA class III) who were not eligible for treatment with cardiac resynchronization therapy, from a statutory health insurance perspective in Germany over a lifetime horizon. METHODS: A decision analytic model was developed using the combination of a decision tree and the Markov process...
August 9, 2018: BMC Cardiovascular Disorders
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