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Fertilization in vitro

Daiana A De Souza, Osman Kaftanoglu, David De Jong, Robert E Page, Gro V Amdam, Ying Wang
The effect of larval nutrition on female fertility in honey bees is a focus of both for scientific studies and for practical applications in beekeeping. In general, morphological traits are standards for classifying queens and workers and for evaluating their quality. In recent years, in vitro rearing techniques have been improved and used in many studies; they can produce queen-like and worker-like bees. Here, we questioned whether queens and workers reared in vitro are the same as queens and workers reared in a natural hive environment...
October 19, 2018: Biology Open
Tomotaka Hara, Airi Kin, Sogo Aoki, Shinsuke Nakamura, Koumei Shirasuna, Takehito Kuwayama, Hisataka Iwata
The present study investigated the vitrification-induced deterioration of mitochondrial functions that may reduce the developmental ability of post-warming bovine embryos. In addition, the effect of supplementation of the culture medium with resveratrol on the mitochondrial functions and post-warming embryonic development was examined. Two days after in vitro fertilization, embryos with 8-12 cells (referred to hereafter as 8-cell embryos) were vitrified and warmed, followed by in vitro incubation for 5 days in a culture medium containing either the vehicle or 0...
2018: PloS One
S Borys, M Brązert, M Jankowski, I Kocherova, K Ożegowska, P Celichowski, M J Nawrocki, W Kranc, A Bryja, M Kulus, M Jeseta, W Pieńkowski, A Bręborowicz, D Bukowska, P Antosik, L Pawelczyk, M T Skowroński, K P Brüssow, M Bruska, M Zabel, M Nowicki, B Kempisty
Before being able to fully participate in the processes associated with its function as a female gamete, the oocyte needs to undergo a range of changes to achieve its mature form. These morphological, biochemical and metabolomic processes are induced by the somatic tissues surrounding the oocyte, through the expression of specific transcription and growth factors. The maturation of the oocyte is highly important for the proceedings that lead to successful fertilization, early embryonic development and implantation...
September 2018: Journal of Biological Regulators and Homeostatic Agents
Molly M Quinn, Flor Juarez-Hernandez, Molly Dunn, Richard Jason Okamura, Marcelle I Cedars, Mitchell P Rosen
PURPOSE: We aimed to explore how patients make decisions regarding use of preimplantation genetic testing for aneuploidy (PGT-A) for in vitro fertilization (IVF). METHODS: This is a cross-sectional survey at an academic medical center. Three hundred subjects initiating an IVF cycle over 8 weeks were asked to complete a validated survey to determine how they decided whether or not to pursue PGT-A. All patients were previously counseled that the primary goal of PGT-A is to maximize pregnancy rates per embryo transfer...
October 18, 2018: Journal of Assisted Reproduction and Genetics
Qingqing Yao, Guangyi Cao, Mingzhe Li, Baixing Wu, Xiaoyun Zhang, Teng Zhang, Jing Guo, Hong Yin, Lanying Shi, Jiayi Chen, Xiang Yu, Lina Zheng, Jinbiao Ma, You-Qiang Su
Producing normal eggs for fertilization and species propagation requires completion of meiosis and protection of the genome from the ravages of retrotransposons. Mutation of Marf1 (meiosis regulator and mRNA stability factor 1) results in defects in both these key processes in mouse oocytes and thus in infertility. MARF1 was predicted to have ribonuclease activity, but the structural basis for the function of MARF1 and the contribution of its putative ribonuclease domain to the mutant oocyte phenotype was unknown...
October 17, 2018: Proceedings of the National Academy of Sciences of the United States of America
Léa Delbos, Elsa Parot-Schinkel, Hady El Hachem, Guillaume Legendre, Philippe Descamps, Lisa Boucret, Véronique Ferré-L'Hotellier, Pauline Jeanneteau, Cécile Dreux, Catherine Morinière, Pascale May-Panloup, Pierre-Emmanuel Bouet
BACKGROUND: To date, there is no consensus on the ideal management strategy of patients with poor ovarian response (POR) to controlled ovarian stimulation (COS) for in vitro fertilization (IVF). Currently, these patients are given the choice of: (1) canceling the cycle; (2) proceeding with COS regardless of the poor response, and performing the oocyte retrieval and transfer of embryos when available; or (3) conversion to an intrauterine insemination (IUI). When the decision to proceed with the COS cycle is taken, it is not clear whether IVF or conversion to IUI is the best choice...
October 17, 2018: Trials
Javier Del Rey, Francisco Vidal, Lorena Ramírez, Nina Borràs, Irene Corrales, Iris Garcia, Olga Martinez-Pasarell, Silvia F Fernandez, Raquel Garcia-Cruz, Aïda Pujol, Alberto Plaja, Itziar Salaverria, Maria Oliver-Bonet, Jordi Benet, Joaquima Navarro
In families at risk from monogenic diseases affected offspring, it is fundamental the development of a suitable Double Factor Preimplantation Genetic Testing (DF-PGT) method for both single-gene analysis and chromosome complement screening. Aneuploidy is not only a major issue in advanced-maternal-age patients and balanced translocation carriers, but also the aneuploidy rate is extremely high in patients undergoing in vitro fertilization (IVF), even in young donors. To adequate NGS technology to the DF-PGT strategy four different whole genome amplification systems (Sureplex, MALBAC, and two multiple displacement amplification systems-MDA) were tested using TruSight One panel on cell lines and blastocyst trophectoderm biopsies-TE...
2018: PloS One
Quanwen Li, Erica Louden, Jordan Zhou, Sascha Drewlo, Jing Dai, Elizabeth Ella Puscheck, Kang Chen, Daniel A Rappolee
Mouse Embryonic Stem Cells (mESC) are unique in their self-renewal and pluripotency. Hypothetically, mESCs model gestational stress effects or stresses of in vitro fertilization/assisted reproductive technologies or drug/environmental exposures that endanger embryos. Testing mESCs stress responses should diminish and expedite in vivo embryo screening. Transgenic mESCs for green fluorescent protein (GFP) reporters of differ-entiation use the promoter for platelet-derived growth factor receptor (Pdgfr)a driving GFP expression to moni-tor hyperosmotic stress-forced mESC proliferation decrease (stunting), and differentiation increase that further stunts mESC population growth...
October 17, 2018: Stem Cells and Development
Barbara Luke, Morton B Brown, Ethan Wantman, David B Seifer, Amy T Sparks, Paul C Lin, Kevin J Doody, Bradley J Van Voorhis, Logan G Spector
PURPOSE: To evaluate the risk of prematurity and infant mortality by maternal fertility status, and for in vitro fertilization (IVF) pregnancies, by oocyte source and embryo state combinations. METHODS: Women in 14 States who had IVF-conceived live births during 2004-13 were linked to their infant's birth and death certificates; a 10:1 sample of non-IVF births was selected for comparison; those with an indication of infertility treatment on the birth certificate were categorized as subfertile, all others were categorized as fertile...
October 17, 2018: Journal of Assisted Reproduction and Genetics
Luis Guzman, D Nuñez, R López, N Inoue, J Portella, F Vizcarra, L Noriega-Portella, L Noriega-Hoces, S Munné
OBJECTIVE: To study if the number of trophectoderm (TE) biopsied cells has an impact on implantation rates. DESIGN: A retrospective cohort study in a single-center study. SETTING: In vitro fertilization center. PATIENTS: Patients who underwent PGT-A from January 2013 to March 2016. In total, 482 vitrified/warmed single embryo transfers were included. INTERVENTIONS: None. MAIN OUTCOME MEASURES: Clinical pregnancies rate, implantation rate...
October 17, 2018: Journal of Assisted Reproduction and Genetics
Maia Brik, Irene Fernández-Buhigas, Aránzazu Martin-Arias, Marina Vargas Terrones, Rubén Barakat, Belén Santacruz
OBJECTIVE: to evaluate differences in maternal weight gain and fetal cardiac function according to exercise during pregnancy METHODS: A randomized controlled trial was performed at Hospital de Torrejón, Madrid, including 120 pregnant women, allocated into Exercise Group (EG) and Control Group (CG). The primary outcome was maternal weight. Secondary outcomes included caesarean section, preterm delivery, induction of labor, birth weight and fetal cardiac function parameters. A sample size of 45 (90 both groups) was planned to detect differences in maternal weight gain of at least 1 Kg, with a power of >80% (>0...
October 17, 2018: Ultrasound in Obstetrics & Gynecology
Marina Nikolaeva, Alina Babayan, Elena Stepanova, Alla Arefieva, Tatiana Dontsova, Veronika Smolnikova, Elena Kalinina, Lubov Krechetova, Stanislav Pavlovich, Gennady Sukhikh
Seminal plasma (SP) is thought to be a crucial factor which affects the expansion of regulatory T cells (Tregs) in female reproductive tract during embryo implantation. We propose that seminal transforming growth factor (TGF) β1 is responsible for local accumulation of circulating Tregs, which manifests as changes in Treg frequency in peripheral blood, whereas seminal interleukin (IL) 18 interferes with TGF-β1-dependent cellular reactions. The purpose of the present study is to determine whether the frequency of circulating Tregs is associated with the levels of seminal cytokines and pregnancy establishment in women exposed to partner's SP during in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycle...
October 16, 2018: Reproductive Sciences
Lucie Gavin-Plagne, Loris Commin, Pierre Bruyère, Samuel Buff, Thierry Joly
Animal-derived products are widely used in sperm cryopreservation for their cryoprotective properties. These components, however, tend to be replaced because of sanitary risks. STEMALPHA.CRYO3 (Ref. 5617; Stem Alpha, Saint-Genis-l'Argentière, France), called "CRYO3," is a chemically defined preservation medium currently used for freezing human tissue and adult stem cells. The aim of this study was to evaluate the effect of a CRYO3-based medium on ram sperm freezing regarding in vitro parameters and in vivo fertility...
October 16, 2018: Biopreservation and Biobanking
Omar Sefrioui, Aicha Madkour, Smahane Aboulmaouahib, Ismail Kaarouch, Noureddine Louanjli
Circulating anti-müllerian hormone (AMH) and antral follicle count (AFC) are the best predictors of IVF outcomes. However, in extreme low AMH range especially for young patients, AMH prediction power loose its specificity to give real idea of pregnancy chance with IVF treatments and good prognosis of an extremely reduced ovarian reserve and expected poor response. Indeed, this retrospective study was conducted to evaluate IVF outcomes in patients following IVF-ICSI program with extremely low AMH levels (≤0...
October 16, 2018: Gynecological Endocrinology
Miranda L Bernhardt, Paula Stein, Ingrid Carvacho, Christopher Krapp, Goli Ardestani, Aujan Mehregan, David M Umbach, Marisa S Bartolomei, Rafael A Fissore, Carmen J Williams
The success of mammalian development following fertilization depends on a series of transient increases in egg cytoplasmic Ca2+ , referred to as Ca2+ oscillations. Maintenance of these oscillations requires Ca2+ influx across the plasma membrane, which is mediated in part by T-type, CaV 3.2 channels. Here we show using genetic mouse models that TRPM7 channels are required to support this Ca2+ influx. Eggs lacking both TRPM7 and CaV 3.2 stop oscillating prematurely, indicating that together they are responsible for the majority of Ca2+ influx immediately following fertilization...
October 15, 2018: Proceedings of the National Academy of Sciences of the United States of America
Barbara Luke, Morton B Brown, Ethan Wantman, Valerie L Baker, Kevin J Doody, David B Seifer, Logan G Spector
BACKGROUND: Over the past two decades the characteristics of women giving birth in the United States and the nature of the births themselves have changed dramatically, with increases in older maternal age, plural births, cesarean deliveries, and conception from infertility treatment. OBJECTIVE: To evaluate the risk of severe maternal morbidity by maternal fertility status, and for IVF pregnancies, by oocyte source and embryo state combinations. STUDY DESIGN: Women in eight States who underwent in vitro fertilization (IVF) cycles resulting in a live birth during 2004-13 were linked to their infant's birth certificates; a 10:1 sample of births from non-IVF deliveries were selected for comparison; those with an indication of infertility treatment on the birth certificate were categorized as subfertile, all others were categorized as fertile...
October 12, 2018: American Journal of Obstetrics and Gynecology
Steven T Bird, Sengwee Toh, Leyla Sahin, Susan E Andrade, Kate Gelperin, Lockwood Taylor, Jaejoon Song, Christian Hampp
Letrozole is an aromatase inhibitor with an unapproved use for ovulation induction with infertility. Because of the proximity of this use to conception, we selected letrozole to explore the effect of imperfect estimation in the pregnancy start on misclassification in measurement of first-trimester exposure. Using electronic health data from the United States Sentinel database (2001-2015), we identified live-birth pregnancies conceived through in-vitro fertilization or intrauterine insemination. The pregnancy start was calculated using 1) a validated algorithm to estimate the last menstrual period (LMP), 2) LMP+14 days (i...
October 15, 2018: American Journal of Epidemiology
Adel Farhi, Saralee Glasser, Shay Frank, Galit Hirsh-Yechezkel, Louise Brinton, Bert Scoccia, Rafael Ron-El, Liat Lerner-Geva, Lidia V Gabis
Objective: To describe development of a methodology for an outcome study of children born following in-vitro fertilization or spontaneously-conceived, as a model for defining normal and below-normal development of school-age children for research purposes. Study Design: The main issues addressed were defining the major health and developmental domains to be investigated, selection of age-appropriate validated instruments, considering time constraints to maximize compliance, and budgetary limitations. The final protocol included a half-hour structured telephone interview with mothers of all 759 children and a 2-h developmental assessment of 294 of them...
2018: Frontiers in Pediatrics
Xin-Jun Wu, Peter Thomas, Yong Zhu
Recent investigations suggest progestin receptor membrane component 1 (PGRMC1) associates with and transports a wide range of molecules such as heme, cytochromes P450, steroids with 21 carbons, membrane progestin receptor alpha (mPRα/Paqr7), epidermal growth factor receptor (EGFR), and insulin receptor. It is difficult to discriminate the true functions of PGRMC1 from the functions of its associated molecules using biochemical and pharmacological approaches. To determine the physiological function(s) of PGRMC1, we generated global knockouts for pgrmc1 ( pgrmc1 -/- ) in zebrafish...
2018: Frontiers in Endocrinology
Xiu-Li Yang, Fei Chen, Xiu-Ying Yang, Guan-Hua Du, Yang Xu
We appreciated receiving comments from Guo (1) on our article (2), which is a meta-analysis on the efficacy of low-molecular-weight heparin (LMWH) on the outcomes of in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) pregnancy in non-thrombophilic women. It highlights that the discussion on the efficacy of LMWH in assisted reproductive technology (ART) is a hot clinical topic. There are various interventions in clinic on promoting the success rate of IVF/ICSI in recent years. Among them, LWMH is a relatively safe drug for fetuses and pregnant women...
October 14, 2018: Acta Obstetricia et Gynecologica Scandinavica
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