Read by QxMD icon Read

Fertilization in vitro

Chenyang Huang, Haixiang Sun, Zhilong Wang, Yang Liu, Xi Cheng, Jingyu Liu, Ruiwei Jiang, Xindong Zhang, Xin Zhen, Jidong Zhou, Linjun Chen, Lijun Ding, Guijun Yan, Yue Jiang
Recurrent implantation failure (RIF) caused by various etiological factors remains a challenge for fertility clinicians using assisted reproductive technology (ART) worldwide. Dysregulation of leukemia inhibitory factor (LIF) in the endometria of women with RIF is involved in impaired endometrial receptivity and embryo adhesion. However, the mechanism through which LIF expression is regulated in women with RIF is still poorly understood. Our previous study noted that the abnormally increased endometrial Krüppel-like factor 12 (KLF12) in RIF women led to impaired decidualization and embryo implantation...
2018: Cell Death Discovery
C W C Cheung, S Saravelos, T Y A Chan, D S Sahota, C C Wang, P W Chung, T C Li
OBJECTIVE: To ascertain whether stress biomarker and psychological indices of stress may predict both conception and miscarriage rates in women undergoing in-vitro fertilization/ intracytoplasmic sperm injection (IVF/ ICSI). DESIGN: Prospective observational study. SETTING: A university-affiliated tertiary hospital. POPULATION OR SAMPLE: Infertility women who were undergoing fresh or frozen IVF/ICSI cycles. METHODS: Subjects were recruited to (1) completed validated psychological questionnaires (visual analogue scale of stress, state trait anxiety inventory, perceived stress scale, fertility problem Inventory, Beck depression inventory, and general health questionnaire), at the time of embryo transfer (ET) and pregnancy testing (PT), and (2) provided saliva samples for alpha amylase (sAA) measurement before and after ET and at PT...
August 14, 2018: BJOG: An International Journal of Obstetrics and Gynaecology
Young Eun Yoon, Hyung Ho Lee, Sung Yul Park, Hong Sang Moon, Dong Suk Kim, Seung-Hun Song, Dae Keun Kim
This study comprises a systematic review and meta-analysis of microsurgical vasoepididymostomy outcomes in epididymal obstructive azoospermia. A comprehensive literature search was performed using Medline, Embase, and the Cochrane library that included all studies related to microsurgical vasoepididymostomy. Keywords included "vasoepididymostomy," "epididymovasostomy," "epididymal obstruction," and "epididymis obstruction." Event rate and risk ratio (RR) were estimated...
August 14, 2018: Asian Journal of Andrology
Lis C Puga Molina, Guillermina M Luque, Paula A Balestrini, Clara I Marín-Briggiler, Ana Romarowski, Mariano G Buffone
In the early 1950s, Austin and Chang independently described the changes that are required for the sperm to fertilize oocytes in vivo . These changes were originally grouped under name of "capacitation" and were the first step in the development of in vitro fertilization (IVF) in humans. Following these initial and fundamental findings, a remarkable number of observations led to characterization of the molecular steps behind this process. The discovery of certain sperm-specific molecules and the possibility to record ion currents through patch-clamp approaches helped to integrate the initial biochemical observation with the activity of ion channels...
2018: Frontiers in Cell and Developmental Biology
Shuji Takabayashi, Takuya Aoshima, Katsuya Kabashima, Kazushi Aoto, Masato Ohtsuka, Masahiro Sato
Zygote-microinjection or in vitro electroporation of isolated zygotes are now widely used methods to produce genome-edited mice. However, these technologies require laborious and time-consuming ex vivo handling of fertilized eggs, including zygote isolation, gene delivery into zygotes and embryo transfer into recipients. We recently developed an alternative method called improved genome-editing via oviductal nucleic acids delivery (i-GONAD), which does not require the above-mentioned ex vivo handing of zygotes, but instead involves intraoviductal instillation of genome-editing components, Cas9 protein and synthetic gRNAs, into the oviducts of pregnant females at the late 1-cell embryo stage under a dissecting microscope and subsequent electroporation...
August 13, 2018: Scientific Reports
Joshua A Halpern, Peter N Schlegel
Limited observational data purporting a benefit of testicular sperm for intracytoplasmic sperm injection failure do not provide sufficient evidence. The first step is to reduce sperm DNA fragmentation in ejaculated sperm through lifestyle modifications, antioxidant therapy, or treatment of underlying abnormalities such as infection or varicocele.
August 10, 2018: European Urology Focus
Yaakov Melcer, Ron Maymon, Eric Jauniaux
PURPOSE OF REVIEW: Vasa previa is a rare disorder of placentation associated with a high rate of perinatal morbidity and mortality when undetected before delivery. We have evaluated the recent evidence for prenatal diagnosis and management of vasa previa. RECENT FINDINGS: Around 85% of cases of vasa previa have one or more identifiable risk factors including in-vitro fertilization, multiple gestations, bilobed, succenturiate or low-lying placentas, and velamentous cord insertion...
August 9, 2018: Current Opinion in Obstetrics & Gynecology
R P Betarelli, M Rocco, M Yeste, J M Fernández-Novell, A Placci, B Azevedo Pereira, M Castillo-Martín, E Estrada, A Peña, M G Zangeronimo, J E Rodríguez-Gil
The aim of this work was to determine the relationship of intracellular reactive oxygen species (ROS) and the disulphide bonds established between sperm proteins with the achievement of capacitation in boar spermatozoa. With this purpose, spermatozoa were incubated in a specifically designed in vitro capacitation medium (CM) in the presence or absence of reduced glutathione (GSH). Incubation of boar spermatozoa in CM for 4 h significantly (p < 0.05) increased free cysteine residues, which is a marker of disrupted disulphide bonds, and also intracellular ROS levels...
August 12, 2018: Andrology
Selma M Mourad, Cate Curtis, Guy Gudex, Margaret Merrilees, John Peek, Lynn Sadler
BACKGROUND: The Patient-Centred Questionnaire-Infertility (PCQ-Infertility) has proven to be a reliable instrument to assess the extent of patient-centredness of fertility care in European countries. AIMS: To validate the PCQ-Infertility in New Zealand (NZ) and to compare results with international experience. MATERIALS AND METHODS: A cross-sectional 46-item questionnaire study among 409 women undergoing publicly funded fertility care (intrauterine insemination or in vitro fertilisation / intracytoplasmic sperm injection) in three fertility clinics in the Northern Auckland region was performed between October 2015 and September 2016...
August 13, 2018: Australian & New Zealand Journal of Obstetrics & Gynaecology
Mert Ulaş Barut, Hakan Çoksüer, Sibel Sak, Murat Bozkurt, Elif Ağaçayak, Uğur Hamurcu, Didem Kurban, Süleyman Eserdağ
BACKGROUND Hypogonadotropic hypogonadism (HH), or secondary hypogonadism, results from reduced secretion of gonadotropins, including follicle-stimulating hormone (FSH) and luteinizing hormone (LH), by the pituitary gland, resulting in lack of production of sex steroids. The aim of this study was to evaluate self-reported sexual function in sexually active women with and without HH using two evaluation methods, the Female Sexual Function Index (FSFI) and the Beck Depression Inventory (BDI). MATERIAL AND METHODS The study recruited 88 women who attended an outpatient in vitro fertilization (IVF) clinic in Turkey for primary infertility, between August 2013 and August 2016...
August 12, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Atsushi Tanaka, Kohta Suzuki, Motoi Nagayoshi, Akihiro Tanaka, Youichi Takemoto, Seiji Watanabe, Satoru Takeda, Minoru Irahara, Naoaki Kuji, Zentaro Yamagata, Ryuzo Yanagimachi
OBJECTIVE: To compare physical and cognitive development of babies born after round spermatid injection (ROSI) with those born after natural conception. DESIGN: Comparison of efficiencies of ROSI and ICSI using testicular spermatozoa, performed in the St. Mother Clinic. Physical and cognitive development of ROSI babies recorded by parents in the government-issued Mother-Child Handbook was checked and verified by attending pediatricians. Data included baby's weight gain and response to parents' voice/gesture...
August 2018: Fertility and Sterility
Caroline R Juneau, Jason M Franasiak, Linnea R Goodman, Diego Marin, Katherine Scott, Scott J Morin, Shelby A Neal, Jeffrey E Juneau, Richard T Scott
OBJECTIVE: To study the prevalence of celiac disease in the infertile population undergoing in vitro fertilization (IVF) and assess outcomes. DESIGN: Prospective cohort study. SETTING: A single infertility center from January 2016 to March 2017. PATIENT(S): Women 18-45 years of age participating in IVF. INTERVENTION(S): Patients had serum tissue transglutaminase (tTG) and endomysial (EMA) IgA testing to screen for celiac disease and completed a 10-question "yes or no" survey to assess their medical history, previous testing, dietary habits, and pertinent symptoms...
August 2018: Fertility and Sterility
(no author information available yet)
Adjuvant immunotherapy treatments in in vitro fertilization (IVF) aim to improve the outcome of assisted reproductive technology (ART) in both the general ART population as well as subgroups such as patients with recurrent miscarriage or implantation failure. The purpose of this guideline is to evaluate the role of immunomodulating therapy in ART. Unfortunately, many of the evaluated therapies lack robust evidence from well-designed adequately powered randomized controlled trials to support their use. Immunotherapies reviewed in the present document are either not associated with improved live-birth outcome in IVF or have been insufficiently studied to make definitive recommendations...
August 2018: Fertility and Sterility
Anna Rita Piras, Giovanni Pietro Burrai, Federica Ariu, Laura Falchi, Maria Teresa Zedda, Salvatore Pau, Sergio Domenico Gadau, Elisabetta Antuofermo, Daniela Bebbere, Sergio Ledda, Luisa Bogliolo
BACKGROUND: Storage conditions during transportation of explanted ovaries are a critical step in setting up fertility preservation protocols in both animal and human fields. Here, we evaluated the effects of ovary storage at 4 °C on the preservation of preantral follicles and oocytes retrieved from antral follicles using the domestic cat as model. METHODS: Ovaries were harvested from fifty-five healthy domestic queens during ovariectomy and stored at 4 °C for 0 (control), 24, 48, 72 and 96 h...
August 10, 2018: Reproductive Biology and Endocrinology: RB&E
P Perumal, S Chang, K K Baruah, N Srivastava
Mithun (Bos frontalis) is a unique domestic free range bovine species of North Eastern hilly regions of India. The present study was designed to assess the seasonal effect of slow release exogenous melatonin (MT) implant on semen quality parameters (SQP) and in vitro zona binding ability (IVZ) of spermatozoa. The experimental animals were divided into Gr I: Control (n = 5) and Gr II: Treatment (n = 5; melatonin implant @ 18mg/50 kg bwt). A total of 20 semen samples/group in winter, spring, autumn and summer seasons (n = 160), twice per week were collected...
August 3, 2018: Theriogenology
Geraldine Seydoux
P granules are RNA/protein condensates in the germline of C. elegans. Genetic analyses have begun to identify the proteins that regulate P granule assembly in the cytoplasm of zygotes. Among them, the RGG-domain protein PGL-3, the intrinsically-disordered protein MEG-3, and the RNA helicase LAF-1 all bind and phase separate with RNA in vitro. We discuss how RNA-induced phase separation, competition with other RNA-binding proteins, and reversible phosphorylation contribute to the asymmetric localization of P granules in the cytoplasm of newly fertilized embryos...
August 7, 2018: Journal of Molecular Biology
Jennifer F Kawwass, Martina L Badell
Infertility is a disease that affects up to 15.5% of reproductive-aged couples. Until the birth of the first neonate born from in vitro fertilization (IVF) in 1978, many infertile couples did not have an opportunity to conceive a biological child. Over the past 40 years, access to and effectiveness of IVF have increased; currently 1.7% of births in the United States result from IVF. As with any medical intervention, potential risk exists. In the case of IVF, both maternal risks (ovarian stimulation, oocyte retrieval, and subsequent pregnancy) and fetal risks that vary based on maternal age and fetal number must be considered...
August 6, 2018: Obstetrics and Gynecology
Tiffany Phyoe-Battaglia, Chantal Bartels, John Nulsen, Daniel R Grow
No abstract text is available yet for this article.
August 9, 2018: Journal of Assisted Reproduction and Genetics
Alyssa Hamlin, Jessica L Bauer, Alex J Polotsky, Shona C Murray
Patients undergoing assisted reproduction are advised to abstain from intercourse to prevent the possibility of multiple pregnancy. If patients do not follow this advice, multiple dizygotic pregnancy or even a heterotopic pregnancy can result. We report the case of a 28-year-old nulliparous female with unexplained infertility who underwent freeze-all vaginal oocyte retrieval. Twenty-one days later she presented with vaginal bleeding (similar to menstruation) and right lower-quadrant pain. The results of ultrasound scanning and a laboratory work-up were consistent with an ectopic pregnancy...
July 2018: Case Reports in Women's Health
Longtai You, Xiaoxv Dong, Boran Ni, Jing Fu, Chunjing Yang, Xingbin Yin, Xin Leng, Jian Ni
Triptolide isolated from the traditional Chinese herb Tripterygium wilfordii Hook F., possesses anti-tumor, anti-fertility, and anti-inflammatory properties. Triptolide-induced hepatotoxicity has continued to engage the attention of researchers. However, not much is yet known about the cytotoxicity of triptolide, and the precise mechanisms involved. In the present study, we investigated the cytotoxicity of triptolide and its underlying mechanisms, using the in vitro model (HepaRG cell). The results demonstrated that triptolide significantly reduced cell viability and induced apoptosis in HepaRG cells in a dose- and time-dependent manner...
2018: Frontiers in Pharmacology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"