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Fertilization in vitro

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https://www.readbyqxmd.com/read/30516976/can-environmentally-relevant-neuroactive-chemicals-specifically-be-detected-with-the-locomotor-response-test-in-zebrafish-embryos
#1
David Leuthold, Nils Klüver, Rolf Altenburger, Wibke Busch
Chemicals considered as neuroactive (such as certain pesticides, pharmaceuticals and industrial chemicals) are among the largest groups of bioactive substances recently detected in European rivers. However, the determination of nervous system-specific effects has been limited using in vitro tests or conventional endpoints including lethality. Thus, neurobehavioral tests using in vivo models (e.g. zebrafish embryo) have been proposed as complementary approaches. To investigate the specificity and sensitivity of a light-dark transition locomotor response (LMR) test in 4 to 5 days post fertilization zebrafish with respect to different modes of action (MoAs), we analyzed a set of 18 environmentally relevant compounds with various anticipated MoAs...
December 5, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/30516870/the-mystery-of-art
#2
Elle C Roberson, Elizabeth Ann L Enninga
One of the first steps towards a successful pregnancy is fertilization, where sperm and oocyte come together to form a zygote. In mammals, this process can occur in vitro, allowing assisted reproductive technologies (ART) to alleviate certain infertilities. ART - which includes in vitro fertilization and gamete donation - accounted for 1.7% of all US births in the US in 2015 (Sunderam et al. 2018). This article is protected by copyright. All rights reserved.
December 5, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/30512223/embryotrophic-effects-of-extracellular-vesicles-derived-from-outgrowth-embryos-in-pre-and-peri-implantation-embryonic-development-in-mice
#3
Jihyun Kim, Jaewang Lee, Tae Bok Lee, Jin Hyun Jun
Recently, many studies have investigated the role of extracellular vesicles (EVs) on reproductive events, including embryo development and death, oviduct-embryo crosstalk, in vitro fertilization and others. The aim of this study was to demonstrate whether outgrowth embryo-derived EVs function as bioactive molecules and regulate mouse embryonic developmental competence in vitro and implantation potential in utero. The EVs from mouse outgrowth embryos on 7.5 days post-coitum were detected and selectively isolated to evaluate the embryotrophic functions on pre-implantation embryos...
December 4, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/30510935/endochin-like-quinolones-exhibit-promising-efficacy-against-neospora-caninum-in-vitro-and-in-experimentally-infected-pregnant-mice
#4
Nicoleta Anghel, Vreni Balmer, Joachim Müller, Pablo Winzer, Adriana Aguado-Martinez, Mona Roozbehani, Sovitj Pou, Aaron Nilsen, Michael Riscoe, J Stone Doggett, Andrew Hemphill
We report on the efficacy of selected endochin-like quinolones (ELQs) against N. caninum tachyzoites grown in human foreskin fibroblasts (HFF), and in a pregnant BALB/c mouse model. Fourteen ELQs were screened against transgenic N. caninum tachyzoites expressing β-galactosidase (Nc-βgal). Drugs were added concomitantly to infection and the values for 50% proliferation inhibition (IC50 ) were determined after 3 days. Three compounds exhibited IC50 values below 0.1 nM, 3 ELQs had IC50 s between 0.1 and 1 nM, for 7 compounds values between 1 and 10 nM were noted, and one compound had an IC50 of 22...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/30508891/effect-of-interventional-embolization-treatment-for-hydrosalpinx-on-the-outcome-of-in-vitro-fertilization-and-embryo-transfer
#5
Xin Hong, Wen-Bin Ding, Rui-Fan Yuan, Jia-Yi Ding, Jie Jin
To explore the effect of interventional embolization treatment for hydrosalpinx on the outcome of in vitro fertilization and embryo transfer (IVF-ET).During the period from January 2013 to January 2015, a total of 129 patients with unilateral or bilateral hydrosalpinx were treated with IVF-ET and selected for retrospective analysis. Seventy-three patients (intervention group) with unilateral or bilateral hydrosalpinx were treated with fallopian tube embolization, which was followed by IVF-ET. During the same period, 56 patients (control group) with unilateral or bilateral hydrosalpinx directly received IVF-ET without receiving any treatment for hydrosalpinx...
November 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/30508652/hysteroscopic-placement-of-platinum-microinsert-in-patients-with-hydrosalpinx-before-in-vitro-fertilization-and-embryo-transfer
#6
Hui Chen, Wanjun Jiang, Ge Lin, Guangxiu Lu, Fei Gong
STUDY OBJECTIVE: To investigate the effectiveness of Tornado and Hilal (Cook Medical, Bloomington, Indiana, USA) hysteroscopic proximal tubal occlusion (HPTO) for hydrosalpinx (HX) treatment prior to in vitro fertilization (IVF) and embryo transfer (ET) in patients with dense pelvic adhesions or low ovarian reserve. DESIGN: Retrospective study of patients treated between May 2014 and May 2016 (Canadian Task Force classification III). SETTING: University-affiliated center...
November 30, 2018: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/30506995/does-blastomere-removal-alter-the-expression-level-of-mir-let7a-and-its-target-genes-following-mouse-embryo-biopsy
#7
Fatemeh Naseri, Sara Hosseini, Marefat Ghaffari Novin, Ahmad Hosseini, Mohammad Hasan Heidari, Mohammad Salehi
Embryo manipulations may cause the misexpression of various genes, most of which play critical roles in the regulation of implantation. This study aimed to evaluate the effects of embryo biopsy on the expression of miR-Let-7a and its gene targets including ErbB4, Tgf-α, Itg-αv, Itg β3 on the implantation of mouse embryo. Embryos were produced by in vitro fertilization followed by blastomere biopsy at the eight-cell stage. The effects of blastomere removal on the expression of genes ErbB4, Tgf-α, Itg αv, Itg β3, and miR-Let-7a as well as the alteration of the blastocyst cell number were compared in both biopsied and non-biopsied groups...
December 2, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/30506864/investigation-of-platelet-rich-plasma-in-increasing-proliferation-and-migration-of-endometrial-mesenchymal-stem-cells-and-improving-pregnancy-outcome-of-patients-with-thin-endometrium
#8
Xiaohan Wang, Ling Liu, Shanmao Mou, Huishan Zhao, Jianye Fang, Yanjie Xiang, Tong Zhao, Tongye Sha, Jie Ding, Cuifang Hao
BACKGROUND: Platelet-rich plasma (PRP) contains abundant growth factors and is gradually used in the field of reproduction. A thin endometrium is recognized as a critical factor in embryo implantation failure. Endometrial mesenchymal stem cells (EnMSCs), which were isolated from human menstrual blood, are highly proliferative and show multiple differentiation capacity. The current study was to investigate the effect of PRP on the proliferation and migration of EnMSCs, and the effectiveness of PRP in the treatment of patients with thin endometrium...
December 3, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/30506768/association-of-the-human-aryl-hydrocarbon-receptor-repressor-ahrr-c-565c-g-transversion-with-male-infertility-a-case-control-study-from-iran
#9
Azadeh Mohammad-Hasani, Abasalt Hosseinzadeh Colagar, Ali Fallah
Dioxins (eg, 2,3,7,8-tetrachlorodibenzo-p-dioxin/TCDD), as environmental endocrine disruptors and toxic carcinogens, can affect male reproductive health. The influence of dioxins is mediated via the aryl hydrocarbon receptor (AhR) pathway and its repressor (AhRR). In this study, we investigated the association of AhRR-c.565C>G transversion polymorphism with male infertility. In a hospital-based case-control study, 221 semen samples (111 infertile and 110 healthy controls) based on World Health Organization guidelines were collected from in vitro fertilization centers of Babol, Iran...
December 2, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/30506306/screening-of-plant-growth-promotion-ability-among-bacteria-isolated-from-field-grown-sorghum-under-different-managements-in-brazilian-drylands
#10
Jéssica Fernanda da Silva, Thaise Rosa da Silva, Indra Elena Costa Escobar, Ana Carla Resende Fraiz, Jonnathan Whiny Moraes Dos Santos, Tailane Ribeiro do Nascimento, João Marcos Rodrigues Dos Santos, Samuel James Windsor Peters, Roseli Freire de Melo, Diana Signor, Paulo Ivan Fernandes-Júnior
Sorghum [Sorghum bicolor (L.) Moench] is a multipurpose grass cultivated in drylands due to its adaptation to drought. However the characteristics of sorghum-associated bacteria are not known in the Brazilian drylands. The aim of this study was to isolate and evaluate the plant growth promotion potential bacteria from field-grown sorghum under two irrigation and manure application levels in a Brazilian semi-arid reagion. Sorghum was irrigated with 3 or 1 mm day-1 and fertilized or not with liquid goat manure...
November 30, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30504940/metabolomic-analysis-of-human-follicular-fluid-potential-follicular-fluid-markers-of-reproductive-aging
#11
Xing-Xing Zhang, Yi Yu, Zhen-Gao Sun, Jing-Yan Song, Ai-Juan Wang
OBJECTIVE: To assess the difference in the metabolomics profiles of follicular fluid between older and younger reproductive-aged women. METHODS: The retrospective study was conducted at the Centre of Reproduction and Genetics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China and comprised patient data related to the period between July and October 2015. Follicular fluid was obtained from male-factor infertility women aged 28-35 years as the younger group A, and those aged 35-42 years as the older group B...
December 2018: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/30503182/neither-male-age-nor-semen-parameters-influence-clinical-pregnancy-or-live-birth-outcomes-from-ivf
#12
Uma Mariappen, Kevin N Keane, Peter M Hinchliffe, Satvinder S Dhaliwal, John L Yovich
Advanced age is an increasing trend for both males and females seeking in vitro fertilization (IVF). This retrospective cohort study investigated the outcomes of 1280 IVF-related treatment cycles, selecting the first treatment for couples utilizing autologous gametes and who underwent single fresh embryo transfer. Males aged 40-49 years had a 52% reduction in normal sperm motility, while it was markedly reduced by 79% at 50 years or older. However, neither semen parameters nor male age were predictive of clinical pregnancy or live birth chance...
November 29, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/30503133/using-the-engagedmd-multimedia-platform-to-improve-informed-consent-for-ovulation-induction-intrauterine-insemination-and-in-vitro-fertilization
#13
Jody Lyneé Madeira, Jennifer Rehbein, Mindy S Christianson, Miryoung Lee, J Preston Parry, Guido Pennings, Steven R Lindheim
OBJECTIVE: To study patient and provider feedback on how a multimedia platform (EngagedMD) helps patients to understand the risks and consequences of in vitro fertilization (IVF), ovulation induction (OI), and intrauterine insemination (IUI) treatments and the impact of the informed consent process. DESIGN: Prospective survey study. SETTING: IVF units in the United States. PATIENT(S): Six-thousand three-hundred and thirty-three patients who viewed the multimedia platform before IVF or OI-IUI treatment at 13 U...
December 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/30503130/effect-of-body-weight-on-early-hormone-levels-in-singleton-pregnancies-resulting-in-delivery-after-in-vitro-fertilization
#14
Rachel B Mejia, Taylor W Cox, Edward B Nguyen, Karen M Summers, Patrick Ten Eyck, Amy E Sparks, Bradley J Van Voorhis
OBJECTIVE: To evaluate which clinical characteristics influence early maternal β-human chorionic gonadotropin (hCG) and progesterone levels in in vitro fertilization (IVF) pregnancies. DESIGN: Retrospective cohort analysis. SETTING: Academic medical center. PATIENT(S): Women with a live birth after single-blastocyst embryo transfer in either a fresh or frozen cycle between 2004 and 2017, comprising 1,282 pregnancies in 1,057 patients...
December 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/30503129/growth-hormone-during-in-vitro-fertilization-in-older-women-modulates-the-density-of-receptors-in-granulosa-cells-with-improved-pregnancy-outcomes
#15
Sheena L P Regan, Phil G Knight, John L Yovich, Frank Arfuso, Arun Dharmarajan
OBJECTIVE: To study the effect of aging and granulosa cell growth hormone receptor (GHR) expression, and the effect of growth hormone (GH) co-treatment during IVF on receptor expression. DESIGN: Laboratory study. SETTING: University. PATIENT(S): A total of 445 follicles were collected from 62 women undergoing standard infertility treatment. INTERVENTION(S): Preovulatory ovarian follicle biopsies of granulosa cells and follicular fluid...
December 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/30503109/ovarian-endometriosis-and-infertility-in-vitro-fertilization-ivf-or-surgery-as-the-first-approach
#16
EDITORIAL
Bruce A Lessey, Stephan Gordts, Olivier Donnez, Edgardo Somigliana, Charles Chapron, Juan A Garcia-Velasco, Jacques Donnez
No abstract text is available yet for this article.
December 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/30503108/women-with-endometrioma-related-infertility-face-a-dilemma-when-choosing-the-appropriate-therapy-surgery-or-in-vitro-fertilization
#17
EDITORIAL
Jacques Donnez
No abstract text is available yet for this article.
December 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/30502403/perfluorononanoic-acid-pfna-alters-lipid-accumulation-in-bovine-blastocysts-after-oocyte-exposure-during-in-vitro-maturation
#18
Ida Hallberg
Perfluorononanoic acid (PFNA) is one of the perfluoroalkyl acids present in human tissues. In this study, effects on early embryo development after PFNA exposure were investigated using the bovine in vitro production system. Oocytes were exposed to PFNA during maturation in vitro (10 µg mL-1 and 0.1 µg mL-1 ), and then fertilized and cultured in parallel with control groups. Developmental parameters (cleavage, blastocyst formation) were followed and embryo quality evaluated (stage, grade). Embryos developed after exposure to 0...
November 28, 2018: Reproductive Toxicology
https://www.readbyqxmd.com/read/30502364/geometric-elastic-and-contractile-relaxation-changes-in-coronary-arterioles-induced-by-vitamin-d-deficiency-in-normal-and-hyperandrogenic-female-rats
#19
Leila Hadjadj, Anna Monori-Kiss, Eszter M Horváth, Andrea Heinzlmann, Attila Magyar, Réka E Sziva, Zsuzsanna Miklós, Éva Pál, János Gál, István Szabó, Zoltán Benyó, György L Nádasy, Szabolcs Várbíró
Vitamin D (VitD) hypovitaminosis and androgen excess (AE) are both risk factors for cardiovascular diseases in fertile women. However, the possible early interaction between AE and VitD status is not clear. Our goal was to describe how VitD status influences early changes in the biomechanical reactivity of small coronary arterioles in adult female rats after transdermal testosterone treatment. Forty-six adolescent, 90-110-gram-weighed female Wistar rats were randomly grouped into 4 groups. Twenty-four animals received an optimal VitD-supplemented diet, from which 12 animals underwent transdermal testosterone treatment...
November 28, 2018: Microvascular Research
https://www.readbyqxmd.com/read/30501453/endometrial-but-not-ovarian-response-is-associated-with-clinical-outcomes-and-can-be-improved-by-prolonged-pituitary-downregulation-in-patients-with-thin-and-medium-endometrium
#20
Jianyuan Song, Xuejiao Sun, Kun Qian
The aim of this study is to investigate the effect of ovarian and endometrial response on live birth rates (LBRs) in young normal and high responders and prolonged pituitary downregulation on endometrial receptivity and clinical outcomes in patients with different endometrial thickness. Between January 2013 and December 2017, 9511 patients underwent first in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycles with age ≤35 years, follicle stimulating hormone < 10 IU/l, and ≥4 retrieved oocytes were conducted...
December 3, 2018: Reproductive Sciences
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