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Semen preservation

G Raad, L Lteif, R Lahoud, J Azoury, J Azoury, J Tanios, M Hazzouri, J Azoury
INTRODUCTION: Human sperm freezing is very widely used for male fertility preservation. This procedure consists in adding cryoprotectants to the spermatozoa followed by cooling and storing the spermatozoa at a subzero temperature. Many standardized cryopreservation media are available on the market. However, these media differ in their chemical composition and there are no sufficient data to optimize their classification. Therefore, the aim of this study was to compare five commercially available sperm cryopreservation media, which have not been compared together, in terms of motility, morphology and DNA integrity...
August 13, 2018: Andrology
José Carlos Ferreira-Silva, Sarah Romini Lima Basto, Marcelo Tigre Moura, Jorge Motta Rocha, Leopoldo Mayer Freitas Neto, José Pompeu Santos Filho, Manoel Lopes Silva Filho, Marcos Antonio Lemos Oliveira
The work described here aimed to verify the efficiency of different extenders for cryopreservation of equine semen using sperm motility and acrosin activity as spermatic parameters. The semen was fractioned into two equal parts and resuspended in an 11% lactose solution in a 1:1 proportion, where it remained for 20 minutes at room temperature. The semen was centrifuged at 600 g for 10 minutes, and after the second centrifugation, each pellet received the freezing extender (Merck or Zorlesco) and was loaded into 4 mL straws...
July 30, 2018: Biopreservation and Biobanking
Gholam Reza Dashti, Zohre Nateghian, Farhad Golshan Iranpour
Sperm motility is the result of transverse movements that exist along its tail. It plays an important role in male fertility. The aim of this study was to evaluate the influence of keeping washed normozoospermic semen samples at 4-6 and 25 °C on the motility of spermatozoa. 26 semen samples of normozoospermic were washed twice in modified Ham's F10 medium. Then, thirteen of the semen samples were kept in refrigerator (4-6 °C) and the remaining samples were stored in incubator (25 °C) for 12 days. On the 0 (immediately after sampling as control group), 1st, 2nd, 5th, 7th and the 12th days, the percentage of fast progressive (grade a), slow progressive (grade b), non-progressive (grade c) and immotile (grade d) sperm cells were calculated for each temperature...
July 27, 2018: Cell and Tissue Banking
A H Alm-Kristiansen, F B Sanderholen, G Bai, K E Waterhouse, E Kommisrud
Motility and energy level in sperm cells are tightly linked, but not totally understood. The aim of this study was to examine whether adenosine triphosphate (ATP) content as a sperm quality parameter for bull semen could give additional information together with viability and motility. The objective was therefore to examine the relationships between alterations in sperm ATP content, motility and viability in bovine semen samples immediately after thawing and following post-thaw incubation at physiological temperature...
July 25, 2018: Reproduction in Domestic Animals, Zuchthygiene
Nicola Zampieri, Francesco Saverio Camoglio
Preserving the fertility potential of adolescent patients with varicocele is still the main outcome of pediatric and adolescent urologysts; the aim of this study is to assess the fertility potential of patients treated for varicocele with testicular hypotrophy in absence of post-operative complications, with complete ipsilateral testicular catch-up growth and normal hormonal values. METHODS: The Authors collected data from adolescents with varicocele aged between 10 and 16 years treated at their Institution between September 2010 and September 2015...
2018: American Journal of Clinical and Experimental Urology
S Akhter, B A Rakha, M S Ansari, S Iqbal, M Khalid
Egg yolk is used as a cryoprotectant in semen preservation. However, its composition varies according to the species which may influence its effectiveness during the freeze-thaw process. Therefore, study was conducted to identify the optimum level of pigeon egg yolk (PEY) in Tris citric acid (TCA) extender for freezability and in vivo fertility of buffalo semen. Semen was collected at weekly intervals for a period of three weeks (replicates) from 6 Nili Ravi buffalo bulls (2 ejaculates/bull/replicate) and diluted with TCA extender (50 × 106 motile spermatozoa ml-1 ) containing 5%, 10%, 15% and 20% PEY or 20% CEY (control) and cryopreserved...
June 23, 2018: Theriogenology
Robert Hermes, Thomas Bernd Hildebrandt, Frank Göritz
At times when rhinoceros are fiercely poached, when some rhinoceros species are closer than ever to extinction, and when the scientific community is in debate over the use of advanced cell technologies as a remaining resort it is time to simplify and improve existing assisted reproduction techniques to enhance breeding and genetic diversity in the living populations under our care. Semen cryopreservation has been performed in all captive rhinoceros species with limited degree of success. Here we tested three freezing extenders, containing different cryoprotectants and various freezing rates for the cryopreservation of rhinoceros sperm from 14 bulls...
2018: PloS One
Federico Remes Lenicov, Ana Luz Paletta, Melina Gonzalez Prinz, Augusto Varese, Clara E Pavillet, Álvaro Lopez Malizia, Juan Sabatté, Jorge Raul Geffner, Ana Ceballos
Inflammatory dendritic cells (DCs) are a distinct subset of DCs that derive from circulating monocytes infiltrating injured tissues. Monocytes can differentiate into DCs with different functional signatures, depending on the presence of environment stimuli. Among these stimuli, transforming growth factor-beta (TGF-β) and prostaglandin E2 (PGE2) have been shown to modulate the differentiation of monocytes into DCs with different phenotypes and functional profiles. In fact, both mediators lead to contrasting outcomes regarding the production of inflammatory and anti-inflammatory cytokines...
2018: Frontiers in Immunology
Dong-Hyuk Jeong, Jeong-Ho Kim, Ki-Jeong Na
The Amur leopard cat (Prionailurus bengalensis euptilurus) is a globally endangered species, and there is thus an urgent need to increase its population. The objectives of this study were to: (1) confirm the utility of urethral catheterization (UC) for semen collection from Amur leopard cats; (2) investigate proper dilution media for fresh semen; and (3) investigate the effectiveness of sperm cryopreservation, including examining the effect of glycerol concentration. Six adult males in captivity (mean weight 5...
July 5, 2018: Theriogenology
Itsuto Hamano, Shingo Hatakeyama, Rika Nakamura, Rie Fukuhara, Daisuke Noro, Toshikazu Tanaka, Tohru Yoneyama, Hayato Yamamoto, Takahiro Yoneyama, Yasuhiro Hashimoto, Takuya Koie, Yoshihito Yokoyama, Chikara Ohyama
OBJECTIVES: To compare semen parameters between patients with testicular cancer and other malignancies using various cut-off values, and to evaluate the correlation between semen parameters and intracytoplasmic sperm injection outcomes. METHODS: We retrospectively investigated semen parameters before cancer treatment in 117 patients with malignancies who cryopreserved sperm at Hirosaki University Hospital between November 1999 and May 2016. We compared semen parameters between patients with testicular cancer and other malignancies (non-testicular cancer), seminoma and non-seminoma, and stage I testicular cancer and stage II/III testicular cancer...
July 2, 2018: International Journal of Urology: Official Journal of the Japanese Urological Association
Rajani K Paul, Krishnappa Balaganur, Davendra Kumar, Syed M K Naqvi
Seminal plasma (SP) is known to induce motility and capacitation in spermatozoa curtailing their lifespan when preserved. Hence, this study was conducted to examine the effects of removal of SP from sperm surface prior to liquid preservation either by high dilution (1/15) or by washing and the poststorage treatment with SP (15% and 25%, v/v) on the quality attributes of liquid-preserved ram semen. Over the period of storage, the rapid motility (66.0% and 71.1% vs. 58.3%), straightness (87.1% and 82.1% vs. 79...
June 28, 2018: Reproduction in Domestic Animals, Zuchthygiene
Alexandra Traila, Delia Dima, Patriciu Achimas-Cadariu, Romeo Micu
In total, 80%-90% of Hodgkin's lymphoma (HL) patients are curable with combination chemoradiotherapy. Due to improvements in therapeutic strategies, 50% of all relapsed/refractory patients may undergo complete clinical responses and have long-term survival. Treatment options for HL are effective, but may have a negative impact on post-chemotherapy fertility. Thus, cryopreservation of semen prior to treatment is recommended for male patients. For female patients, assisted reproductive techniques (ART) consult and fertility preservation should be offered as a therapeutical option...
2018: Cancer Management and Research
Jemma A Moody, Kamran Ahmed, Tet Yap, Suks Minhas, Majid Shabbir
OBJECTIVES: The aim of the present paper was to determine the impact of testicular cancer (TC) and its treatments on fertility and to review the current management options for the infertile patient with TC, both before diagnosis and after treatment, with the aim of providing practical recommendations to update contemporary guidelines and standardize clinical practice. PATIENTS AND METHODS: Searches were conducted for relevant articles on Pubmed and Google Scholar between 2000 and 2017, with additional articles sourced from reference lists of included publications...
June 19, 2018: BJU International
Paula Domingo, Maite Olaciregui, Noelia González, Ignacio DE Blas, Lydia Gil
The purpose of this research was to assess whether the presence of seminal plasma (SP) can improve sperm quality of rabbit spermatozoa stored at 16ºC for 72 hours and moreover evaluate the cryoprotectant effects of glycerol, N-N-Dimethylformamide (DMF), and N-methyl-2-pyrrolidone (NMP). Semen samples were pooled and divided in eight fractions. Four of them were diluted with INRA (extender A), INRA with 6% glycerol (extender B), INRA with 6% DMF (extender C), or INRA with 6% NMP (extender D), respectively. The other four fractions were centrifuged, and the supernatant was removed in order to eliminate SP...
June 13, 2018: Experimental Animals
Nathalie Rives, Aurélie Rives, Christine Rondanino, Mireille Castanet, Ariane Cuny, Louis Sibert
Spermatozoa have occasionally been identified in ejaculate of adult Klinefelter syndrome (KS) patients but very exceptionally in KS adolescents. Spermatozoa can also be retrieved in testicular tissue of KS adolescents. The testis may also harbor spermatogonia and noncompletely differentiated germ cells. Neither clinical features nor hormonal parameters could predict germ cell recovery in KS adults or adolescents. No predictive factors can actually demonstrate that early diagnosis of KS would allow increasing the chance of sperm retrieval even if it has been suggested that semen quality may decline with age in KS patients...
2018: Endocrine Development
Erika Aline Ribeiro Dias, Suzane Peres Campanholi, Guilherme Fazan Rossi, Camila de Paula Freitas Dell'Aqua, José Antonio Dell'Aqua, Frederico Ozanam Papa, Mariana Furtado Zorzetto, Claudia Cristina Paro de Paz, Letícia Zoccolaro Oliveira, Maria Eugênia Zerlotti Mercadante, Fabio Morato Monteiro
Semen cryopreservation comprises different steps, among them are the cooling and freezing rates which significantly influence the quality of thawed sperm. Different systems with variable freezing rates are used for freezing bull semen in the field, with a consequence of variable success rates. The objective of this study was to compare different systems for freezing bull semen in the field. Five cooling methods of semen and two methods for the subsequent freezing phase (5 × 2 factorial scheme) were used...
May 23, 2018: Animal Reproduction Science
Ashley D Franklin, William T Waddell, Karen L Goodrowe
Cryopreserving genetic resources is becoming increasingly important for species management. In the zoo-based red wolf (Canis rufus) population, inbreeding continues to increase in the absence of new founders. Through banking sperm, we preserve genetic diversity and create the ability to decrease inbreeding accumulation in the future. The quality and quantity of banked sperm can be affected by cryopreservation media and semen collection methods. This study's objectives were to further optimize semen extender used for red wolf sperm cryopreservation, investigate effects of post-thaw holding temperature, and to determine if urethral catheterization is an effective method for semen collection in this species...
August 2018: Theriogenology
Mahmoud Mima, David Greenwald, Samuel Ohlander
PURPOSE OF REVIEW: Global industrialization has increased population exposure to environmental toxins. A global decline in sperm quality over the last few decades raises questions about the adverse impact of environmental toxins on male reproductive health. RECENT FINDINGS: Multiple animal- and human-based studies on exposure to environmental toxins suggest a negative impact on semen quality, in terms of sperm concentration, motility, and/or morphology. These toxins may exert estrogenic and/or anti-androgenic effects, which in turn alter the hypothalamic-pituitary-gonadal axis (HPGA), induce sperm DNA damage, or cause sperm epigenetic changes...
May 17, 2018: Current Urology Reports
Graham Luke Machen, Stephanie E Harris, Erin T Bird, Monica L Brown, Dale A Ingalsbe, Milaida M East, Michelle Reyes, Thomas J Kuehl
Purpose: Cryopreservation of sperm from human semen has been available since the 1950s. The actual utilization of available cryopreservation technology has been infrequently reported. We set out to examine the utilization and outcomes of cryopreserved sperm cells based on the indication for storage. Materials and Methods: A dataset was developed from retrospective review. The purposes for cryopreservation, eventual utilization, and outcomes of use for insemination were recorded...
May 2018: Investigative and Clinical Urology
P M Ramesh, A S Vickram, T B Sridharan, R Parameswari, K Archana, S Nithya, A Mishika
  BACKGROUND: Human sperm cell preservation is an important part of assisted reproductive technology (ART). OBJECTIVE: This study aimed to find the essential and significant components in semen preservation extender required to prolong the shelf life of human spermatozoa. MATERIALS AND METHODS: By using the statistical tool 'Plackett-Burman design' the significant components present in E4 extender (formulated in our previous study) was determined by reducing the unacceptable large number of trial experiments from the full factorial method...
November 2017: Cryo Letters
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