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Semen preservation

Amanda J Adeleye, Garrett Reid, Chia-Ning Kao, Evelyn Mok-Lin, James F Smith
OBJECTIVE: To report on the semen quality parameters in transgender women who pursued semen cryopreservation either in the presence or absence of gender affirming hormonal medication. MATERIALS AND METHODS: This retrospective cohort study was conducted as a chart review of consecutive transgender women presenting for semen cryopreservation between January 1, 2012 and March 31, 2018. Demographic data and semen parameters were assessed. Primary outcomes were the semen parameters in subjects with either no exposure, previous exposure or current exposure to gender affirming hormonal medication RESULTS: Twenty-eight patients presented for semen cryopreservation and produced 69 specimens...
October 9, 2018: Urology
Nataliia M Onul, Eleonora M Biletska, Viktor P Stus, Mykyta Yu Polion
OBJECTIVE: Introduction: The problem of preserving and strengthening of the reproductive health of the population in conditions of the industrial regions is becoming one of the key. The aim: Тo determine whether blood and semen concentration of lead in fertile and infertile men without occupational exposure to lead is associated with semen quality and reproductive outcome. PATIENTS AND METHODS: Materials and methods: Clinical and hygienic assessment of the reproductive health was carried out in 192 men of the Dnipropetrovsk region, living in the industrial and control, conditionally «clean» city...
2018: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
Ozlem Okutman, Maroua Ben Rhouma, Moncef Benkhalifa, Jean Muller, Stéphane Viville
PURPOSE: This review provides an update on the genetics of male infertility with emphasis on the current state of research, the genetic disorders that can lead to non-syndromic male infertility, and the genetic tests available for patients. METHODS: A comprehensive review of the scientific literature referenced in PubMed was conducted using keywords related to male infertility and genetics. The search included articles with English abstracts appearing online after 2000...
September 26, 2018: Journal of Assisted Reproduction and Genetics
José A B Bezerra, Andréia M Silva, Patrícia C Sousa, Lívia B Campos, Érica C G Praxedes, Luana G P Bezerra, Thibério S Castelo, Ana L P Souza, Alexandre R Silva
SummaryThe aim of this study was to establish a functional freezing-thawing protocol for epididymal sperm of collared peccaries (Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP®-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37°C/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed...
September 17, 2018: Zygote: the Biology of Gametes and Early Embryos
Natnita Mattawanon, Jessica B Spencer, David A Schirmer, Vin Tangpricha
Gender affirming procedures adversely affect the reproductive potential of transgender people. Thus, fertility preservation options should be discussed with all transpeople before medical and surgical transition. In transwomen, semen cryopreservation is typically straightforward and widely available at fertility centers. The optimal number of vials frozen depends on their reproductive goals and treatment options, therefore a consultation with a fertility specialist is optimal. Experimental techniques including spermatogonium stem cells (SSC) and testicular tissue preservation are technologies currently under development in prepubertal individuals but are not yet clinically available...
September 15, 2018: Reviews in Endocrine & Metabolic Disorders
Qian Li, Weike Shaoyong, Yu Li, Meijie Chen, YaMei Hu, Bin Liu, GongShe Yang, JianHong Hu
The use of antioxidants is an important aspect in the preservation of boar semen. Oligomeric proanthocyanidins (OPC) are an effective natural antioxidants that scavenges free radicals and reactive oxygen species (ROS). This study was designed to investigate the antioxidative effect of OPC on boar semen quality during liquid preservation at 17 °C. The effects of different concentrations of OPC on sperm quality variables, antioxidant effects and fertility were analyzed in this experiment. Semen collected from six Guanzhong-Black boars and was diluted with Beltsville thawing solution (BTS)...
September 1, 2018: Animal Reproduction Science
Sara Keshtgar, Shekufeh Bagheri, Bahareh Ebrahimi
Cooling method was proposed to maintain the sperm quality for several days. Nevertheless, during this procedure, sperm is encountered to "cold shock", and its quality decreases time-dependently. This study was designed to improve the in vitro sperm preservation methods. Thirty normal semen samples were examined in Shiraz, Iran, 2017. Fifteen samples were incubated at 22-27 °C and 15 samples were cooled moderately to 4 °C. Each sample was divided into five subgroups; control, solvent, 200 μM Trolox, 40 μM Coenzyme Q10, and 10 mM ATP...
September 2018: Iranian Journal of Medical Sciences
Martin Schulze, Jana Schäfer, Christian Simmet, Markus Jung, Christoph Gabler
The presence of pathogenic bacteria in ejaculates has been a topic in boar semen preservation over the last decades. Since little information is available on commensal bacteria in boar semen, the aim of the present study was to identify commensal lactobacilli in fresh cryopreserved boar semen and to examine their influence on boar semen quality. Therefore, 111 boar ejaculates were investigated for the presence of Lactobacillus species. Thirty samples (27%) contained viable Lactobacillus species (e.g. L. amylovorus, L...
2018: PloS One
Ding-Ming Li, Bo Liu, Yang Xian, Li-Juan Ying, Yan Zheng, Min Jiang, Fu-Ping Li, Huan-Xun Yue
Objective: To search for an effective method for cryopreservation of rare human sperm (RHS) by comparing the effect of RHS cryopreservation technology with that of conventional cryopreservation technology on post-thaw sperm from patients with severe oligozoospermia. METHODS: Semen samples obtained from 82 patients with severe oligozoospermia were preserved by RHS cryopreservation technology, and another 24 samples cryopreserved by conventional technology, the former divided into groups A (sperm concentration < 1×10⁶/ml, n = 54) and B (1×10⁶/ml ≤ sperm concentration < 5×10⁶/ml, n = 28), and the latter included in group C (sperm concentration < 15×106 /ml, n = 24)...
August 2018: Zhonghua Nan Ke Xue, National Journal of Andrology
Jarosław Król, Daniel Żarski, Gergely Bernáth, Katarzyna Palińska-Żarska, Sławomir Krejszeff, Artur Długoński, Ákos Horváth
The objectives of the present study were to determine values for semen quality variables in the Eurasian perch (i.e., osmolality of seminal plasma as well as sperm motility characteristics analyzed with CASA system) in response to (1) the method of milt collection (stripping or catheterization) and (2) experimental contamination of catheterized semen with urine (0%, 5%, 10%, 20%, 30% and 50% of contamination). Additionally, the effect of short-term chilled storage of experimentally contaminated semen (during the 24 h post semen collection period) on motility characteristics was investigated...
October 2018: Animal Reproduction Science
Patricio Ulloa-Rodríguez, Pablo Contreras, Kelly Dumorné, Manuel Lee-Estevez, Rommy Díaz, Elías Figueroa, Iván Valdebenito, Jennie Risopatrón, Jorge G Farías
Patagonian blenny (E. maclovinus) is a marine species recently placed in captivity and which are potentially farmable. Understanding and improving its sperm capacity to withstand short-term storage conditions is a key element of initiating an artificial propagation program for this species. The aim of this study is to evaluate the ultrastructure and quality of E. maclovinus sperm during refrigerated storage. To address this objective, scanning electron microscopy (SEM), cytofluorimetric analysis (membrane integrity; reactive oxygen species generation; mitochondrial membrane potential) and cell respiration/mitochondrial-function analysis (ATP content; oxygen consumption) could be useful for optimizing or improving management for artificial reproduction of this species...
October 2018: Animal Reproduction Science
Aurore Thélie, Amélie Bailliard, François Seigneurin, Tatiana Zerjal, Michèle Boichard, Elisabeth Blesbois
For the past 50 yr, practices for ex situ preservation of endangered breeds have been extended. Semen and primordial germ cells, gonadic tissues have been frozen to create genetic stocks of chicken genetic diversity in cryobanks. Semen cryopreservation stays the preferred method since it is not invasive. Many protocols have been developed to cryopreserve chicken semen, but they give highly variable success rate. The aim of the present study was to standardize and prove the effectiveness of semen long-term storage for the restitution of lost families...
August 24, 2018: Poultry Science
Catharyn Stern, Franca Agresta
With improved survival rates from cancer, young people can expect to lead a normal life, including having their own children. However, cancer or other serious disease itself, and more often its treatment, often leads to a significant reduction in fertility or premature gonadal insufficiency. There is increasing acknowledgement for the importance of fertility preservation (FP) options to be discussed and offered to young people whose fertility is at risk, ideally before the gonadotoxic therapy begins. FP options currently include oocyte, embryo and ovarian tissue cryopreservation; ovarian protection during chemotherapy and semen, sperm and testicular tissue cryopreservation...
August 1, 2018: Best Practice & Research. Clinical Obstetrics & Gynaecology
Mohamed Badawy Abdel-Naser, Uwe Wollina, Mechthild Lohan, Christos C Zouboulis, Andreas Altenburg
Congenital bilateral absence of vas deferens (CBAVD) results in obstructive azoospermia in which testicular function, such as spermatogenesis, is preserved. Bilateral testicular biopsy is not only diagnostic but also therapeutic as retrieved spermatozoa are usually cryopreserved for assisted reproduction. In this case report, CBAVD was confirmed in a 24-year-old azoospermic man on the basis of persistent azoospermia associated with low semen volume, absent fructose and normal hormonal levels, ultrasonographically (absent seminal vesicles) and histologically (intact spermatogenesis)...
August 22, 2018: Andrologia
Reza Safari Asl, Farid Shariatmadari, Mohsen Sharafi, Mohammad Amir Karimi Torshizi, Abdolhossein Shahverdi
Cockerel semen is sensitive to cooling, which limits chilled storage of semen for more than 24 h. Results of artificial insemination with cold-stored semen are not desirable. This study was conducted to evaluate the effects of dietary fish oil and vitamin E (vitE) for cold-storage of rooster semen and its effects on parameters of semen during 48 h cooling preservation. Roosters were assigned into four dietary treatments; 1) control group received a basal diet, 2) vitE group received a basal diet supplemented with 200 mg/kg vitE, 3) fish oil group (FO) received a basal diet supplemented with 2% fish oil and 4) fish oil and vitE group received a basal diet supplemented with 2% fish oil and 200 mg/kg vitE (FO + vitE)...
August 18, 2018: Cryobiology
Monika Saini, Naresh L Selokar, Prabhat Palta, Manmohan S Chauhan, Radhey S Manik, Suresh K Singla
The first birth of a cloned animal produced through the Handmade cloning (HMC) technique was reported more than 15 years ago in cattle. This method of somatic cell nuclear transfer (SCNT) has subsequently been evolving as a much simpler alternative to the classical micromanipulator-based SCNT. Several farm animal species such as cattle, buffalo, pigs, sheep, and goats have been successfully cloned using HMC. In buffalo, HMC technique is now well established, and several births of cloned calves have been reported by us...
August 9, 2018: Animal Reproduction Science
G Raad, L Lteif, R Lahoud, J Azoury, J Azoury, J Tanios, M Hazzouri, J Azoury
INTRODUCTION: Human sperm freezing is very widely used for male fertility preservation. This procedure consists in adding cryoprotectants to the spermatozoa followed by cooling and storing the spermatozoa at a subzero temperature. Many standardized cryopreservation media are available on the market. However, these media differ in their chemical composition and there are no sufficient data to optimize their classification. Therefore, the aim of this study was to compare five commercially available sperm cryopreservation media, which have not been compared together, in terms of motility, morphology and DNA integrity...
August 13, 2018: Andrology
José Carlos Ferreira-Silva, Sarah Romini Lima Basto, Marcelo Tigre Moura, Jorge Motta Rocha, Leopoldo Mayer Freitas Neto, José Pompeu Santos Filho, Manoel Lopes Silva Filho, Marcos Antonio Lemos Oliveira
The work described here aimed to verify the efficiency of different extenders for cryopreservation of equine semen using sperm motility and acrosin activity as spermatic parameters. The semen was fractioned into two equal parts and resuspended in an 11% lactose solution in a 1:1 proportion, where it remained for 20 minutes at room temperature. The semen was centrifuged at 600 g for 10 minutes, and after the second centrifugation, each pellet received the freezing extender (Merck or Zorlesco) and was loaded into 4 mL straws...
July 30, 2018: Biopreservation and Biobanking
Gholam Reza Dashti, Zohre Nateghian, Farhad Golshan Iranpour
Sperm motility is the result of transverse movements that exist along its tail. It plays an important role in male fertility. The aim of this study was to evaluate the influence of keeping washed normozoospermic semen samples at 4-6 and 25 °C on the motility of spermatozoa. 26 semen samples of normozoospermic were washed twice in modified Ham's F10 medium. Then, thirteen of the semen samples were kept in refrigerator (4-6 °C) and the remaining samples were stored in incubator (25 °C) for 12 days. On the 0 (immediately after sampling as control group), 1st, 2nd, 5th, 7th and the 12th days, the percentage of fast progressive (grade a), slow progressive (grade b), non-progressive (grade c) and immotile (grade d) sperm cells were calculated for each temperature...
July 27, 2018: Cell and Tissue Banking
Anne Hege Alm-Kristiansen, Fride B Standerholen, Godlove Bai, Karin E Waterhouse, Elisabeth Kommisrud
Motility and energy level in sperm cells are tightly linked, but not totally understood. The aim of this study was to examine whether adenosine triphosphate (ATP) content as a sperm quality parameter for bull semen could give additional information together with viability and motility. The objective was therefore to examine the relationships between alterations in sperm ATP content, motility and viability in bovine semen samples immediately after thawing and following post-thaw incubation at physiological temperature...
July 25, 2018: Reproduction in Domestic Animals, Zuchthygiene
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