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Semen preservation

Mustafa Numan Bucak, Mustafa Bodu, Nuri Başpınar, Şükrü Güngör, Pınar İli, Begimay Acibaeva, Tohid Rezaei Topraggaleh, Şükrü Dursun
Objective: The purpose of the present study was to assess the effects of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen. Materials and Methods: In this experimental study, sixty ejaculates from six mature Merino rams were used. In experiment 1, the ejaculates were diluted in base extender contained ellagic acid at 0 (control), 0.5, 1, and 2 mM. In experiment 2, ebselen at 0 (control), 10, 20, and 40 μM were added to the extender...
April 2019: Cell Journal
Chunrong Lv, Guoquan Wu, Qionghua Hong, Guobo Quan
The cryotolerance of farm animal spermatozoa varies according to their specific features, such as size, shape, and lipid composition. Thus, it is impossible to develop a standardized freezing procedure for different kinds of livestock species. The establishment of an efficient semen cryopreservation procedure will facilitate long-term conservation of small ruminant genetic resources and extension of artificial insemination in daily production. Different from sheep, goat seminal plasma contains a phospholipase, which can affect spermatozoa viability through interaction with milk or egg yolk...
November 30, 2018: Biopreservation and Biobanking
Alessandro Mameli, Carmine Maria Scudiero, Giovanni Delogu, Maria Elena Ghiani
We performed forensic investigations on a handkerchief containing seminal fluid deposited 100 years earlier. The aim was to verify the possibility to achieve a complete genetic profile exceeding the limit of success reached until today with a partial semen stain profile stored up to 50 years. The current forensic methods were carried out: Alternative Light Source (ALS), Prostate-Specific Antigen (PSA) Test, autosomal and Y-chromosome Short Tandem Repeats (STRs). ALS inspection was a crucial method in the success of the analysis...
November 19, 2018: Journal of Forensic and Legal Medicine
Martyna Polit, Sylwia Prochowska, Wojciech Niżański
The aim of the study was to compare the characteristics of chinchilla epididymal sperm: fresh, stored at liquid state and cryopreserved. Epididymal spermatozoa obtained from 11 males were assessed for subjective motility, concentration, motility parameters measured by CASA, viability, morphology, membrane integrity, acrosome integrity, mitochondrial potential, lipid peroxidation, chromatin structure, apoptotic changes and capacitation. Then half of the spermatozoa were stored at 5°C for 30 hr, and the second half was cryopreserved...
November 2018: Reproduction in Domestic Animals, Zuchthygiene
Tácia Gomes Bergstein-Galan, Romildo Romualdo Weiss, Luiz Ernandes Kozicki
Multiple ovulation and embryo transfer (MOET) is an important tool in the sheep industry for increasing numbers of genetically superior individuals. The objective of this study was to evaluate the effect of semen source (frozen or fresh), the number of embryo collection procedures for each donor (NECP), the season in which embryo transfer and collection was performed, and the age and breed of the donor, on the number of recovered embryos and pregnancy rates after embryo transfer. The Alamos Genetics' flushing station database was used...
November 25, 2018: Reproduction in Domestic Animals, Zuchthygiene
A Orzołek, Ł Zasiadczyk, P Wysocki, W Kordan, P Krysztofiak
The aim of this study was to measure the NO level in boar semen held in a liquid state and to determine its putative relation to spermatozoa motility, plasma membrane integrity, mitochondrial membrane potential and ATP content. Generally, the percentage of spermatozoa which generated nitric oxide gradually increased, while NO level in the surrounding medium declined during the liquid preservation. NO generation in semen preserved in BTS was higher as compared to those in Androhep®Plus. We demonstrated the positive correlation between the NO level in fresh spermatozoa and their quality...
June 2018: Polish Journal of Veterinary Sciences
Danielle B L Oliveira, Giuliana S Durigon, Érica A Mendes, Jason T Ladner, Robert Andreata-Santos, Danielle B Araujo, Viviane F Botosso, Nicholas D Paola, Daniel F L Neto, Marielton P Cunha, Carla T Braconi, Rúbens P S Alves, Monica R Jesus, Lennon R Pereira, Stella R Melo, Flávio S Mesquita, Vanessa B Silveira, Luciano M Thomazelli, Silvana R Favoretto, Franciane B Almonfrey, Regina C R M Abdulkader, Joel M Gabrili, Denise V Tambourgi, Sérgio F Oliveira, Karla Prieto, Michael R Wiley, Luís C S Ferreira, Marcos V Silva, Gustavo F Palacios, Paolo M A Zanotto, Edison L Durigon
We followed the presence of Zika virus (ZIKV) in four healthy adults (two men and two women), for periods ranging from 78 to 298 days post symptom onset. The patients were evaluated regarding the presence of the virus in different body fluids (blood, saliva, urine and semen), development of immune responses (including antibodies, cytokines and chemokines), and virus genetic variation within samples collected from semen and urine during the infection course. The analysis was focused primarily on the two male patients who shed the virus for up to 158 days after the initial symptoms...
November 7, 2018: Viruses
Ana Paula Pinoti Pavaneli, Marina da Silva Passarelli, Flávia Vieira de Freitas, Gisele Mouro Ravagnani, Mariana Andrade Torres, Simone Maria Massami Kitamura Martins, Marc Yeste, André Furugen Cesar de Andrade
Seminal plasma (SP) plays a vital role in the maintenance of sperm function and integrity along with being involved in their communication with the female reproductive tract. Under in vitro conditions, although it is generally accepted that boar semen is better preserved when SP is diluted (extended) or removed (cryopreserved), its role during storage is not completely elucidated. In this context, the current study sought to determine the role of SP during storage of boar spermatozoa at 17 °C for 72 h...
October 25, 2018: Theriogenology
David Martin-Hidalgo, Ana Hurtado de Llera, Violeta Calle-Guisado, Lauro Gonzalez-Fernandez, Luis Garcia-Marin, M Julia Bragado
AMP-activated protein kinase AMPK regulates cellular energy by controlling metabolism through the inhibition of anabolic pathways and the simultaneous stimulation of catabolic pathways. Given its central regulator role in cell metabolism, AMPK activity and its regulation have been the focus of relevant investigations, although only a few studies have focused on the AMPK function in the control of spermatozoa's ability to fertilize. This review summarizes the known cellular roles of AMPK that have been identified in mammalian spermatozoa...
October 23, 2018: International Journal of Molecular Sciences
Koji Kawai, Hiroyuki Nishiyama
Chemotherapy-induced gonadal dysfunction resulting in transient or persistent infertility depends on the type of drugs and cumulative dose, and it is an important long-term complication, especially for adolescent and young adult (AYA) cancer patients. Due to its importance, a clinical practice guideline for fertility preservation in childhood and AYA cancer patients was published by the Japan Society of Clinical Oncology (JSCO) in 2017. Although the precise mechanisms remain unclear, several studies reported that the cancer itself, not the cancer treatment, adversely affected semen quality...
October 23, 2018: International Journal of Clinical Oncology
Lucie Gavin-Plagne, Loris Commin, Pierre Bruyère, Samuel Buff, Thierry Joly
Animal-derived products are widely used in sperm cryopreservation for their cryoprotective properties. These components, however, tend to be replaced because of sanitary risks. STEMALPHA.CRYO3 (Ref. 5617; Stem Alpha, Saint-Genis-l'Argentière, France), called "CRYO3," is a chemically defined preservation medium currently used for freezing human tissue and adult stem cells. The aim of this study was to evaluate the effect of a CRYO3-based medium on ram sperm freezing regarding in vitro parameters and in vivo fertility...
October 16, 2018: Biopreservation and Biobanking
Amanda J Adeleye, Garrett Reid, Chia-Ning Kao, Evelyn Mok-Lin, James F Smith
OBJECTIVE: To report on the semen quality parameters in transgender women who pursued semen cryopreservation either in the presence or absence of gender affirming hormonal medication. MATERIALS AND METHODS: This retrospective cohort study was conducted as a chart review of consecutive transgender women presenting for semen cryopreservation between January 1, 2012 and March 31, 2018. Demographic data and semen parameters were assessed. Primary outcomes were the semen parameters in subjects with either no exposure, previous exposure or current exposure to gender affirming hormonal medication RESULTS: Twenty-eight patients presented for semen cryopreservation and produced 69 specimens...
October 9, 2018: Urology
Nataliia M Onul, Eleonora M Biletska, Viktor P Stus, Mykyta Yu Polion
OBJECTIVE: Introduction: The problem of preserving and strengthening of the reproductive health of the population in conditions of the industrial regions is becoming one of the key. The aim: Тo determine whether blood and semen concentration of lead in fertile and infertile men without occupational exposure to lead is associated with semen quality and reproductive outcome. PATIENTS AND METHODS: Materials and methods: Clinical and hygienic assessment of the reproductive health was carried out in 192 men of the Dnipropetrovsk region, living in the industrial and control, conditionally «clean» city...
2018: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
Ozlem Okutman, Maroua Ben Rhouma, Moncef Benkhalifa, Jean Muller, Stéphane Viville
PURPOSE: This review provides an update on the genetics of male infertility with emphasis on the current state of research, the genetic disorders that can lead to non-syndromic male infertility, and the genetic tests available for patients. METHODS: A comprehensive review of the scientific literature referenced in PubMed was conducted using keywords related to male infertility and genetics. The search included articles with English abstracts appearing online after 2000...
September 26, 2018: Journal of Assisted Reproduction and Genetics
José A B Bezerra, Andréia M Silva, Patrícia C Sousa, Lívia B Campos, Érica C G Praxedes, Luana G P Bezerra, Thibério S Castelo, Ana L P Souza, Alexandre R Silva
SummaryThe aim of this study was to establish a functional freezing-thawing protocol for epididymal sperm of collared peccaries (Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP®-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37°C/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed...
August 2018: Zygote: the Biology of Gametes and Early Embryos
Natnita Mattawanon, Jessica B Spencer, David A Schirmer, Vin Tangpricha
Gender affirming procedures adversely affect the reproductive potential of transgender people. Thus, fertility preservation options should be discussed with all transpeople before medical and surgical transition. In transwomen, semen cryopreservation is typically straightforward and widely available at fertility centers. The optimal number of vials frozen depends on their reproductive goals and treatment options, therefore a consultation with a fertility specialist is optimal. Experimental techniques including spermatogonium stem cells (SSC) and testicular tissue preservation are technologies currently under development in prepubertal individuals but are not yet clinically available...
September 2018: Reviews in Endocrine & Metabolic Disorders
Qian Li, Weike Shaoyong, Yu Li, Meijie Chen, YaMei Hu, Bin Liu, GongShe Yang, JianHong Hu
The use of antioxidants is an important aspect in the preservation of boar semen. Oligomeric proanthocyanidins (OPC) are an effective natural antioxidants that scavenges free radicals and reactive oxygen species (ROS). This study was designed to investigate the antioxidative effect of OPC on boar semen quality during liquid preservation at 17 °C. The effects of different concentrations of OPC on sperm quality variables, antioxidant effects and fertility were analyzed in this experiment. Semen collected from six Guanzhong-Black boars and was diluted with Beltsville thawing solution (BTS)...
November 2018: Animal Reproduction Science
Sara Keshtgar, Shekufeh Bagheri, Bahareh Ebrahimi
Cooling method was proposed to maintain the sperm quality for several days. Nevertheless, during this procedure, sperm is encountered to "cold shock", and its quality decreases time-dependently. This study was designed to improve the in vitro sperm preservation methods. Thirty normal semen samples were examined in Shiraz, Iran, 2017. Fifteen samples were incubated at 22-27 °C and 15 samples were cooled moderately to 4 °C. Each sample was divided into five subgroups; control, solvent, 200 μM Trolox, 40 μM Coenzyme Q10, and 10 mM ATP...
September 2018: Iranian Journal of Medical Sciences
Martin Schulze, Jana Schäfer, Christian Simmet, Markus Jung, Christoph Gabler
The presence of pathogenic bacteria in ejaculates has been a topic in boar semen preservation over the last decades. Since little information is available on commensal bacteria in boar semen, the aim of the present study was to identify commensal lactobacilli in fresh cryopreserved boar semen and to examine their influence on boar semen quality. Therefore, 111 boar ejaculates were investigated for the presence of Lactobacillus species. Thirty samples (27%) contained viable Lactobacillus species (e.g. L. amylovorus, L...
2018: PloS One
Ding-Ming Li, Bo Liu, Yang Xian, Li-Juan Ying, Yan Zheng, Min Jiang, Fu-Ping Li, Huan-Xun Yue
Objective: To search for an effective method for cryopreservation of rare human sperm (RHS) by comparing the effect of RHS cryopreservation technology with that of conventional cryopreservation technology on post-thaw sperm from patients with severe oligozoospermia. METHODS: Semen samples obtained from 82 patients with severe oligozoospermia were preserved by RHS cryopreservation technology, and another 24 samples cryopreserved by conventional technology, the former divided into groups A (sperm concentration < 1×10⁶/ml, n = 54) and B (1×10⁶/ml ≤ sperm concentration < 5×10⁶/ml, n = 28), and the latter included in group C (sperm concentration < 15×106 /ml, n = 24)...
August 2018: Zhonghua Nan Ke Xue, National Journal of Andrology
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