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Amniotic tissue

Carolina Gonzales da Silva, Carlos Frederico Martins, Heidi Christina Bessler, Álvaro Moraes da Fonseca Neto, Tereza Cristina Cardoso, Maurício Machaim Franco, Anelise Dos Santos Mendonça, Ligiane de Oliveira Leme, José Renato Junqueira Borges, Juaci Vitoria Malaquias, Sônia Nair Báo
The aim of this work was investigate the methylation and hydroximethylation status of mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) and fibroblasts (FIB-control) and verify the effect of trichostatin A (TSA) on gene expression and development of cloned bovine embryos produced using these cells. Characterization of MSC from two animals (BOV1 and BOV2) was performed by flow cytometry, immunophenotyping and analysis of cellular differentiation genes expression. The cells were used in the nuclear transfer in the absence or presence of 50 nM TSA for 20 h in embryo culture...
October 14, 2018: Reproduction in Domestic Animals, Zuchthygiene
Elisa Borghi, Valentina Massa, Marco Severgnini, Grazia Fazio, Laura Avagliano, Elena Menegola, Gaetano Pietro Bulfamante, Giulia Morace, Francesca Borgo
The widely accepted dogma of intrauterine sterility and initial colonization of the newborn during birth has been blurred by recent observations of microbial presence in meconium, placenta, and amniotic fluid. Given the importance of a maternal-derived in utero infant seeding, it is crucial to exclude potential environmental or procedural contaminations and to assess fetal colonization before parturition. To this end, we analyzed sterilely collected intestinal tissues, placenta, and amniotic fluid from rodent fetuses and tissues from autoptic human fetuses...
October 11, 2018: Reproductive Sciences
Lawrence Lau, Dimitrios Papanagnou, Elaine Smith, Crystal Waters, Elizabeth Teixeira, Xiao Chi Zhang
Resuscitative hysterotomy is a daunting and rarely performed procedure in the emergency department (ED). Given the paucity of clinical exposure to this intervention, resuscitative hysterotomy is an ideal opportunity for simulation-mediated deliberate practice. The authors propose a novel training program using a homegrown, realistic, simulation device as a means to practice resuscitative hysterotomy. Two high-fidelity, tissue-based task-trainer models were constructed and tested on a convenience sample of 14 emergency medicine (EM) residents...
2018: Advances in Simulation
Ya-Ping Song, Yuan-Hua Chen, Lan Gao, Peng Wang, Xi-Lu Wang, Biao Luo, Jian Li, De-Xiang Xu
AIMS: The aim of the study was to investigate the effects of high-fat diets before pregnancy and/or during pregnancy on fetal development. MAIN METHODS: Female mice were fed with standard diets (SD) or high-fat diets (HFD). After 12 weeks, females were mated. In the SD + SD and HFD + SD groups, pregnant mice were fed with standard diets. In the SD + HFD and HFD + HFD groups, pregnant mice were fed with high-fat diets. All pregnant mice were sacrificed on gestational day (GD) 16...
October 6, 2018: Life Sciences
Nilusha Malmuthuge, Philip J Griebel
Recent next generation sequencing studies on host-associated microbiomes generated debatable conclusions regarding the central dogma of fetal gut sterility. These observations challenge the concepts that microbial colonization of the gut begins during and after birth as well as the concept of antigen-independent prenatal maturation of mucosal-associated lymphoid tissue in ruminants and humans. The placental barrier varies markedly among mammalian species with mice and humans having haemochorial placentas (fetal tissue in direct contact with maternal blood) versus epitheliochorial placentation (maternal and fetal blood separated by six tissue layers) in ruminants...
September 26, 2018: Veterinary Immunology and Immunopathology
Sandeep Dhall, Malathi Sathyamoorthy, Jin-Qiang Kuang, Tyler Hoffman, Matthew Moorman, Anne Lerch, Vimal Jacob, Steven Michael Sinclair, Alla Danilkovitch
Human amniotic membrane (AM) has a long history of clinical use for wound treatment. AM serves as a wound protective barrier maintaining proper moisture. AM is anti-inflammatory, anti-microbial and antifibrotic, and supports angiogenesis, granulation tissue formation and wound re-epithelialization. These properties of AM are attributed to its native extracellular matrix, growth factors, and endogenous cells including mesenchymal stem cells. Advances in tissue preservation have helped to overcome the short shelf life of fresh AM and led to the development of AM products for clinical use...
2018: PloS One
Kara M Rood, Catalin S Buhimschi, Guomao Zhao, Emily A Oliver, Taryn Summerfield, Mert Ozan Bahtiyar, Irina A Buhimschi
Preterm prelabor rupture of membranes (PPROM), which can precede or follow intra-amniotic infection/inflammation (IAI) is a poorly understood pregnancy complication. Tenascin-X (TNX) is a connective tissue extracellular matrix protein that regulates fibrillogenesis of collagens I, III and V. Our goal was to investigate the presence and level of soluble TNX (sTNX) in amniotic fluid (AF) and TNX expression in reproductive tissues of pregnancies complicated by PPROM and IAI. We prospectively recruited 334 women, pregnant with singletons that had a clinically indicated amniocentesis for genetic karyotyping, lung maturity testing, and rule-out IAI in the presence or absence of PPROM...
October 2, 2018: Biology of Reproduction
Fereshteh Dorazehi, Mohammad Nabiuni, Hanieh Jalali
Scaffolds derived from decellularized tissues provide a natural microenvironment for cell culture. Embryonic cerebrospinal fluid (e-CSF) contains factors which play vital roles in the development of the nervous system. This research was aimed to survey the effect of Wistar rat e-CSF on neural differentiation of bone marrow derived mesenchymal stem cells (BM-MSCs) cultured on the human amniotic membrane (AM). BM-MSCs were collected from femurs and tibias, and were cultured in Dulbecco's Modified Eagle's Medium...
2018: International Journal of Molecular and Cellular Medicine
Cita Rosita Sigit Prakoeswa, Febrina D Pratiwi, Nanny Herwanto, Irmadita Citrashanty, Diah Mira Indramaya, Dwi Murtiastutik, Hari Sukanto, Fedik A Rantam
BACKGROUND: Photoaging is a complex biologic process that affects various layers of the skin with the major damage seen in the connective tissue of the dermis. Although rarely fatal, photoaging can significantly contribute to a loss of quality of life; therefore, it deserves attention. Researchers are continually searching for new compounds to develop rejuvenation therapies. Nowadays, the stem cell population has been discovered, and it shows capability to rejuvenate the skin. MATERIAL AND METHODS: This was an analytic experimental research conducted on a total of 48 women who were randomised into two groups; 24 women received amniotic membrane stem cell-conditioned medium (AMSC-CM) and the other 24 women received normal saline (NS)...
September 28, 2018: Journal of Dermatological Treatment
Alejandro Navas, Fátima Sofía Magaña-Guerrero, Alfredo Domínguez-López, César Chávez-García, Graciela Partido, Enrique O Graue-Hernández, Francisco Javier Sánchez-García, Yonathan Garfias
Acute ocular chemical burns are ophthalmic emergencies requiring immediate diagnosis and treatment as they may lead to permanent impairment of vision. The clinical manifestations of such burns are produced by exacerbated innate immune response via the infiltration of inflammatory cells and activation of stromal fibroblasts. New therapies are emerging that are dedicated to repair mechanisms that improve the ocular surface after damage; for example, transplantation of stem cells (SC) has been successfully reported for this purpose...
September 10, 2018: Stem Cells Translational Medicine
Gurjeet Jutley, Elizabeth Yang, Phillip Bloom
BACKGROUND: The surgical management of patients with uncontrolled glaucoma and scleroderma is challenging, as the hostile ocular surface poses a challenge to surgery. A serious complication is tube erosion, with the risk of subsequent endophthalmitis. Here, we present a novel technique of harvesting autologous tissue to successfully manage recurrent tube extrusion. CASE PRESENTATION: MG is a 60-year-old Arabic lady diagnosed with scleroderma, that was previously managed with systemic corticosteroids...
September 14, 2018: BMC Ophthalmology
Karim Ben M'Barek, Walter Habeler, Alexandra Plancheron, Mohamed Jarraya, Olivier Goureau, Christelle Monville
Several pathological conditions of the eye affect the functionality and/or the survival of the retinal pigment epithelium (RPE). These include some forms of retinitis pigmentosa (RP) and age-related macular degeneration (AMD). Cell therapy is one of the most promising therapeutic strategies proposed to cure these diseases, with already encouraging preliminary results in humans. However, the method of preparation of the graft has a significant impact on its functional outcomes in vivo. Indeed, RPE cells grafted as a cell suspension are less functional than the same cells transplanted as a retinal tissue...
September 6, 2018: Journal of Visualized Experiments: JoVE
Lihong Zhu, Fei Luo, Wenjing Hu, Yang Han, Yuezhu Wang, Huajun Zheng, Xiaokui Guo, Jinhong Qin
The idea that healthy uterine cavity is sterile is challenged nowadays. It is still debatable whether the bacteria present in the uterine cavity during pregnancy are residents or invaders. To reveal microbiome composition and its characteristics in the womb of pregnant women, 41 decidual tissue samples and 64 amniotic fluid samples were taken from pregnant Chinese women. DNA extraction was followed by pyrosequencing of the hypervariable V4 region of the 16S rDNA gene to characterize womb microbiome. Both types of samples had low diversity microbiome with Enterobacteriaceae being the dominant phylotypes at family level...
2018: Frontiers in Microbiology
Anna Lange-Consiglio, Barbara Lazzari, Flavia Pizzi, Alessandra Stella, Alessia Girani, Arianna Quintè, Fausto Cremonesi, Emanuele Capra
Conditioned medium (CM) and microvesicles (MVs) are produced using different protocols: CM is collected following 12-96 hours of cell culture without renewal of tissue culture medium, whilst MVs are collected after overnight cell culture. For future comparative studies in regenerative medicine looking at efficacy of CM and MVs, it is important to understand how the quality of cell secretions is affected by culture. The aim of this study was to evaluate whether the duration of culture influences the micro-RNAs (miRNAs) cargo of equine amniotic-derived cells (AMCs) and their MVs...
September 20, 2018: Tissue Engineering. Part C, Methods
Graciane Petre, Patrick Lorès, Hervé Sartelet, Aurélie Truffot, Brice Poreau, Sandrine Brandeis, Guillaume Martinez, Véronique Satre, Radu Harbuz, Pierre F Ray, Florence Amblard, Françoise Devillard, Gaëlle Vieville, Francois Berger, Pierre-Simon Jouk, Daniel Vaiman, Aminata Touré, Charles Coutton, Marie Bidart
We report findings from a male fetus of 26 weeks' gestational age with severe isolated intrauterine growth restriction (IUGR). Chromosomal microarray analysis (CMA) on amniotic fluid cells revealed a 1.06-Mb duplication in 19q13.42 inherited from the healthy father. This duplication contains 34 genes including ZNF331, a gene encoding a zinc-finger protein specifically imprinted (paternally expressed) in the placenta. Study of the ZNF331 promoter by methylation-specific-multiplex ligation-dependent probe amplification (MS-MLPA) showed that the duplicated allele was not methylated in the fetus unlike in the father's genome, suggesting both copies of the ZNF331 gene are expressed in the fetus...
September 16, 2018: Clinical Genetics
Alvin L Young, Di Cao, Wai Kit Chu, Tsz Kin Ng, Yolanda W Y Yip, Vishal Jhanji, Chi Pui Pang
Pterygium is a fibrovascular subepithelial growth of degenerative tissue over the limbus. It is a common condition worldwide that is especially prevalent in tropical countries within the "pterygium belt." Its exact etiology remains to be elucidated; however, it is strongly associated with exposure to ultraviolet light. The high expression levels of tumor protein p53 (TP53) observed in laboratory studies of pterygium seem to contradict the fast-growing nature of its clinical behavior, and TP53 mutations have been suggested...
November 2018: Cornea
Yongjun Zheng, Shiqing Zheng, Xiaoming Fan, Li Li, Yongqiang Xiao, Pengfei Luo, Yingying Liu, Li Wang, Zhenci Cui, Fei He, Yuhuan Liu, Shichu Xiao, Zhaofan Xia
Human amniotic epithelial cells (hAECs) are nontumorigenic, highly abundant, and low immunogenic and possess multipotent differentiation ability, which make them become ideal alternative stem cell source for regenerative medicine. Previous studies have demonstrated the therapeutic potential of hAECs in many tissue repairs. However, the therapeutic effect of hAECs on diabetic wound healing is still unknown. In this study, we injected hAECs intradermally around the full-thickness excisional skin wounds of db/db mice and found that hAECs significantly accelerated diabetic wound healing and granulation tissue formation...
2018: Stem Cells International
David J Eve, Paul R Sanberg, Leonora Buzanska, Anna Sarnowska, Krystyna Domanska-Janik
Human somatic stem cells can be identified and isolated from different types of tissues and are grouped here based on their developmental maturation and ability to undergo neural differentiation. The first group will represent afterbirth somatic tissues, which are perinatal stem cells including placental blood and tissue, amniotic fluid and tissue, and umbilical cord blood- and umbilical cord tissue-derived cells. The second group of cells discussed in this chapter is the adult stem cells, generally those in a transient period of development, thus placing them in the special position of transitioning from the perinatal to young somatic tissue, and they include the menstrual blood-, the peripheral blood-, and the bone marrow-derived stem cells...
2018: Results and Problems in Cell Differentiation
Jenn D Bullard, Jennifer Lei, Jeremy J Lim, Michelle Massee, Anna M Fallon, Thomas J Koob
Chronic wounds are a significant health care problem with serious implications for quality of life because they do not properly heal and often require therapeutic intervention. Amniotic membrane allografts have been successfully used as a biologic therapy to promote soft tissue healing; however, the umbilical cord, another placental-derived tissue, has also recently garnered interest because of its unique composition but similar placental tissue origin. The aim of this study was to characterize PURION® PLUS Processed dehydrated human umbilical cord (dHUC) and evaluate the biological properties of this tissue that contribute to healing...
September 10, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Diane Van Opstal, Karin E M Diderich, Marieke Joosten, Lutgarde C P Govaerts, Joke Polak, Marjan Boter, Jasper J Saris, Wai Yee Cheung, Stefanie van Veen, Robert van de Helm, Attie T J I Go, Maarten F C M Knapen, Dimitri N M Papatsonis, Anneke Dijkman, Femke de Vries, Robert-Jan H Galjaard, Lies H Hoefsloot, Malgorzata I Srebniak
OBJECTIVE: Non-invasive prenatal testing (NIPT) detects placental chromosome aberrations. When amniocentesis reveals a normal karyotype, confined placental mosaicism (CPM) may be assumed. In order to confirm this, placental cytogenetic studies were performed. METHOD: NIPT was conducted in the course of the Dutch TRIDENT study. Placentas of 10 cases with NIPT results indicating an autosomal trisomy and showing a normal (N = 9) or low mosaic karyotype (N = 1) in amniotic fluid (AF) were investigated...
September 6, 2018: Prenatal Diagnosis
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