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Amniotic tissue

Mario Ledda, Enrico D'Emilia, Maria Grazia Lolli, Rodolfo Marchese, Claudio De Lazzari, Antonella Lisi
Cell therapy is an innovative strategy for tissue repair, since adult stem cells could have limited regenerative ability as in the case of myocardial damage. This leads to a local contractile dysfunction due to scar formation. For these reasons, refining strategy approaches for "in vitro" stem cell commitment, preparatory to the "in vivo" stem cell differentiation, is imperative. In this work, we isolated and characterized at molecular and cellular level, human Amniotic Mesenchymal Stromal Cells (hAMSCs) and exposed them to a physical Extremely Low Frequency Electromagnetic Field (ELF-EMF) stimulus and to a chemical Nitric Oxide treatment...
August 8, 2018: International Journal of Molecular Sciences
Lisa M Rogers, Anjali P Anders, Ryan S Doster, Elizabeth A Gill, Juan S Gnecco, Jacob M Holley, Tara M Randis, Adam J Ratner, Jennifer A Gaddy, Kevin Osteen, David M Aronoff
PROBLEM: Bacterial chorioamnionitis causes adverse pregnancy outcomes, yet host-microbial interactions are not well characterized within gestational membranes. The decidua, the outermost region of the membranes, is a potential point of entry for bacteria ascending from the vagina to cause chorioamnionitis. We sought to determine whether paracrine communication between decidual stromal cells and macrophages shaped immune responses to microbial sensing. METHOD OF STUDY: Decidual cell-macrophage interactions were modeled in vitro utilizing decidualized, telomerase-immortalized human endometrial stromal cells (dTHESCs) and phorbol ester-differentiated THP-1 macrophage-like cells...
August 7, 2018: American Journal of Reproductive Immunology: AJRI
Samuel Kogan, Adit Sood, Mark S Granick
INTRODUCTION: Recent advances in the preservation and processing of amnion/chorion tissue have dramatically increased the bioavailability of these wound healing factors as well as the shelf life of their related tissue products, allowing for a surge in clinical use. Many studies, including basic science, clinical trials, and randomized controlled trials, have emerged examining the biologic properties of amnion/chorion membrane products and their efficacy in wound healing. OBJECTIVE: A literature review was conducted regarding the safety and efficacy of amniotic membrane adjuncts...
June 2018: Wounds: a Compendium of Clinical Research and Practice
Shuhong Wu, Zhili Xiao, Jinlin Song, Min Li, Wenhua Li
BACKGROUND: The aim of this study is to evaluate the effects of recombinant human bone morphogenetic protein 2 (rhBMP-2), human amnion mesenchymal stem cells (hAMSCs), and nanohydroxyapatite/collagen/poly(l-lactide) (nHAC/PLA) in tissue engineering to provide potential approaches for periodontal bone regeneration. METHODS: hAMSCs were isolated from discarded amniotic membrane samples and cultured in vitro. Alkaline phosphatase (ALP) staining and alizarin red staining were performed to evaluate the osteoblast (OB) differentiation ability of hAMSCs...
July 25, 2018: International Journal of Molecular Sciences
Dominika Wróbel-Dudzińska, Jorge Alio, Alejandra Rodriguez, Ewa Suchodoła-Ratajewicz, Ewa Kosior-Jarecka, Beata Rymgayłło-Jankowska, Agnieszka Ćwiklińska-Haszcz, Tomasz Żarnowski
Purpose: Platelet-rich plasma (PRP) is an autologous blood product without preservatives and rich in proteins and growth factors which make it possible for cells to differentiate, proliferate, and migrate, thus stimulating healing and regeneration of tissues. The aim of this study was to evaluate the efficiency of autologous platelet-rich plasma in the treatment of neurotrophic keratopathy. Methods: The study group consists of 25 patients with nonhealing corneal ulcers due to herpes simplex or herpes zoster infection and facial nerve or trigeminal nerve paralysis as a result of a neurosurgical operation caused by a tumour or stroke...
2018: Journal of Ophthalmology
Daniel G Eichberg, Sheikh C Ali, Simon S Buttrick, Ricardo J Komotar
BACKGROUND: In cranial neurosurgery, primary watertight dural closure is the standard method of post-craniotomy dural repair. However, cerebrospinal fluid (CSF) leaks, pseudomeningoceles, postoperative infections, and dural scarring are possible complications, even when a meticulous technique is implemented. For this reason, materials that enhance the dura's ability to create a watertight seal, inhibit the inflammatory response, and prevent disease transmission are sought. Dehydrated amniotic membrane (DAM) allograft appears to facilitate these properties, as studies have shown that it improves wound healing, prevents scar tissue formation, promotes epithelialization, and inhibits bacterial growth...
May 7, 2018: Curēus
Teresa Victoria, Abhay S Srinivasan, Jennifer Pogoriler, Portia A Kreiger, Pablo Laje, Edward R Oliver, Enrico Danzer, Ann M Johnson, Julie S Moldenhauer, William H Peranteau, N Scott Adzick
BACKGROUND: At fetal MR, congenital lung lesions are usually T2 hyperintense with respect to normal lung parenchyma. Some lesions, however, demonstrate unusual patterns of T2 hypointensity, sometimes in a rosette-like pattern. These lesions usually present a diagnostic conundrum. OBJECTIVE: To evaluate the imaging findings and pathological characterization of fetal solid lung lesions with elements showing T2-hypointense signal with respect to lung. MATERIALS AND METHODS: This is a retrospective study of lung lesions with elements showing T2 hypointensity treated prenatally and postnatally at our center and with available pathological evaluation...
July 14, 2018: Pediatric Radiology
Marc Oria, Rebeca L Figueira, Federico Scorletti, Lourenco Sbragia, Kathryn Owens, Zhen Li, Bedika Pathak, Maria U Corona, Mario Marotta, Jose L Encinas, Jose L Peiro
Spina bifida aperta is a congenital malformation characterized by the failure of neural tube closure resulting in an unprotected fetal spinal cord. The spinal cord then undergoes progressive damage, likely due to chemical and mechanical factors related to exposure to the intrauterine environment. Astrogliosis in exposed spinal cords has been described in animal models of spina bifida during embryonic life but its relationship with neuroinflammatory processes are completely unknown. Using a retinoic acid-induced rat model of spina bifida we demonstrated that, when exposed to amniotic fluid, fetal spinal cords showed progressive astrogliosis with neuronal loss at mid-gestation (E15) compared to unexposed spinal cords...
July 13, 2018: Scientific Reports
Sabiniano Roman, Christopher Hillary, Brenda Narice, Anthony J Bullock, Dilly Oc Anumba, Sheila MacNeil
Preterm premature rupture of fetal membranes is a leading cause of preterm delivery. Preterm labour can compromise fetal survival, and even if a pregnancy affected by preterm premature rupture of fetal membrane continues, major complications associated with leakage of amniotic fluid and risk of infection can affect the normal development and survival of the baby. There are limited management options for preterm premature rupture of fetal membrane other than delivery of the baby if ascending infection (chorioamnionitis) is suspected...
January 1, 2018: Journal of Biomaterials Applications
Daniel G Eichberg, Sheikh C Ali, Simon S Buttrick, Ricardo J Komotar
BACKGROUND: Primary watertight dural closure is the preferred method of postcraniotomy dural repair. However, even when ideal technique is implemented, postoperative infection, cerebrospinal fluid (CSF) leaks, pseudomeningoceles, and dural scarring are possible complications. For this reason, materials that augment the dura's ability to create a watertight seal, prevent disease transmission, and inhibit inflammatory response are sought. Dehydrated amniotic membrane (DAM) allograft appears to fulfil these requirements as it has several beneficial properties that aid wound healing, including promotion of epithelialization, scar tissue prevention, and inhibition of bacterial growth...
July 10, 2018: British Journal of Neurosurgery
Ibrahim Inan Harbiyeli, Elif Erdem, Seyda Erdogan, Yurdun Kuyucu, Sait Polat, Meltem Yagmur
PURPOSE: To investigate changes in conjunctival tissue of conjunctivochalasis (CCh) patients and to determine the relationship between pathological findings and localization of loose conjunctiva. METHODS: Our study included nineteen eyes of 19 patients who were referred to Cukurova University Ophthalmology Department based on ocular surface symptoms and CCh detected in ocular examination. Amniotic membrane was applied after conjunctival excision as surgical treatment...
July 10, 2018: International Ophthalmology
Sarumathi G, Siti Solehah Z, Siti Fatmah A, Mohamad Nabil Ia, Rajasegaran M, Hanis Nazihah Hasmad, Mohd Reusmaazran Bin Yusof, Salina H, Haslinda Aa, Y Lokanathan
Amniotic membrane has the potential to be used as scaffold in various tissue engineering applications. However, increasing its biostability at the same time maintaining its biocompatibility is important to enhance its usage as a scaffold. This studied characteristics genipin-crosslinked amniotic membrane as a bioscaffold. Redundant human amniotic membranes (HAM) divided into native (nAM), decellularized (dAM) and genipin-crosslinked (clAM) groups. The dAM and clAM group were decellularized using thermolysin (TL) and sodium hydroxide (NaOH) solution...
July 8, 2018: Journal of Biomaterials Science. Polymer Edition
Lauren Richardson, Ramkumar Menon
Amnion epithelial cell (AEC) shedding causes microfractures in human placental membranes during gestation. However, microfractures are healed to maintain membrane integrity. To better understand the cellular mechanisms of healing and tissue remodeling, scratch assays were performed using primary AECs derived from normal term not in labor membranes. AECs were grown under different conditions: i) normal cultures (control), ii) oxidative stress (OS) induction by cigarette smoke extract (CSE), iii) co-treatment of CSE and antioxidant N-acetyl-l-cysteine, and iv) treatment with amniotic fluid (AF)...
July 6, 2018: American Journal of Pathology
Bhupesh Bagga, Swapna Reddy Motukupally, Ashik Mohamed
PURPOSE: To study the clinical and microbiological profile of microbial keratitis in Stevens-Johnson syndrome (SJS). STUDY DESIGN: Case series. PARTICIPANTS: Patients with SJS who developed microbial keratitis. METHODS: Medical records and microbiological data of patients with SJS who developed microbial keratitis from January 1991 to December 2012 were reviewed. We analysed the type of causative organisms and their antibiotic susceptibility along with the clinical pattern and responses to medications in this group of patients...
July 4, 2018: Ocular Surface
Ítala Keane Rodrigues Dias, Cicera Luciana da Silva Sobreira, Rosa Maria Grangeiro Martins, Kelly Fernanda Silva Santana, Maria do Socorro Vieira Lopes, Emanuella Silva Joventino, Maria Corina Amaral Viana
The objective of this study was to integrate findings related to Zika virus from the scientific literature. An integrative review of Medline was conducted, and data search was performed using the Health Sciences Descriptor Zika virus and the following filters: full texts available; studies in humans; full articles; and publications in Portuguese, French, English, and Spanish. After application of the filters, followed by reading of the titles, abstracts, and full texts, 44 studies were included in the review, for which content analysis was performed...
May 2018: Revista da Sociedade Brasileira de Medicina Tropical
Adele S Ricciardi, Raman Bahal, James S Farrelly, Elias Quijano, Anthony H Bianchi, Valerie L Luks, Rachael Putman, Francesc López-Giráldez, Süleyman Coşkun, Eric Song, Yanfeng Liu, Wei-Che Hsieh, Danith H Ly, David H Stitelman, Peter M Glazer, W Mark Saltzman
Genetic diseases can be diagnosed early during pregnancy, but many monogenic disorders continue to cause considerable neonatal and pediatric morbidity and mortality. Early intervention through intrauterine gene editing, however, could correct the genetic defect, potentially allowing for normal organ development, functional disease improvement, or cure. Here we demonstrate safe intravenous and intra-amniotic administration of polymeric nanoparticles to fetal mouse tissues at selected gestational ages with no effect on survival or postnatal growth...
June 26, 2018: Nature Communications
Lu Zeng, Stephen M Pederson, R Daniel Kortschak, David L Adelson
Background: Transposable elements (TEs) are primarily responsible for the DNA losses and gains in genome sequences that occur over time within and between species. TEs themselves evolve, with clade specific LTR/ERV, LINEs and SINEs responsible for the bulk of species-specific genomic features. Because TEs can contain regulatory motifs, they can be exapted as regulators of gene expression. While TE insertions can provide evolutionary novelty for the regulation of gene expression, their overall impact on the evolution of gene expression is unclear...
2018: Mobile DNA
Aurora Campo, Anne-Gaëlle Lafont, Benjamin Lefranc, Jérôme Leprince, Hervé Tostivint, Nédia Kamech, Sylvie Dufour, Karine Rousseau
In mammals, neurokinin B (NKB) is a short peptide encoded by the gene tac3 . It is involved in the brain control of reproduction by stimulating gonadotropin-releasing hormone (GnRH) neurons, mainly via kisspeptin. We investigated tac3 genes and peptides in a basal teleost, the European eel, which shows an atypical blockade of the sexual maturation at a prepubertal stage. Two tac3 paralogous genes ( tac3a and tac3b ) were identified in the eel genome, each encoding two peptides (NKBa or b and NKB-related peptide NKB-RPa or b)...
2018: Frontiers in Endocrinology
Mario Barilani, Federica Banfi, Silvia Sironi, Enrico Ragni, Salomé Guillaumin, Francesca Polveraccio, Lorenzo Rosso, Monica Moro, Giuseppe Astori, Michela Pozzobon, Lorenza Lazzari
Human multipotent mesenchymal stromal cells (MSC) are isolated from a plethora of tissue sources for cell therapy purposes. In 2006, the International Society for Cellular Therapy (ISCT) published minimal guidelines to define MSC identity. Nevertheless, many independent studies demonstrated that cells meeting the ISCT criteria possessed heterogeneous phenotypes and functionalities, heavily influenced by culture conditions. In this study, human MSC derived from many adult (bone marrow and adipose tissue) or fetal (cord blood, Wharton's jelly, umbilical cord perivascular compartment and amniotic fluid) tissues were investigated...
June 18, 2018: Scientific Reports
Isabel M Gimeno, Ayanna Glaize, Aneg L Cortes
The effect of two Marek's disease (MD) vaccines on the chicken embryo immune responses were evaluated. Transcription of interferon (IFN-α, IFN-β, IFN-λ, and IFN-γ) and interferon-I receptors (IFN-AR1 and IFN-AR2), as well as transcription of toll like receptors (TLR-3, TLR-7, and TLR-21) were evaluated in the bursa, thymus, spleen and lung of 1-day-old chickens that had been vaccinated with HVT, CVI988, or sham inoculated at embryonic day 18 (ED18). Each vaccine had a unique effect on the transcription of the evaluated genes and it differs among tissues...
July 2018: Veterinary Immunology and Immunopathology
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