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Amniotic tissue

Thidathip Wongsurawat, Piroon Jenjaroenpun, Niracha Athipanyasilp, Bualan Kaewnapan, Nattawat Leelahakorn, Nasikarn Angkasekwinai, Wannee Kantakamalakul, Ruengpung Sutthent, David W Ussery, Navin Horthongkham, Intawat Nookaew
We present here the complete genome sequences of Zika virus strains isolated from aborted fetal tissue (brain and placenta) and amniotic fluid of a microcephaly patient in Thailand in 2017. The virus genomes that were sequenced have an average length of 10,807 nucleotides.
September 2018: Microbiology resource announcements
Carolina M Malgarin, Roman Nosach, Predrag Novakovic, Muhammad Suleman, Andrea Ladinig, Susan E Detmer, Daniel J MacPhee, John C S Harding
Although porcine reproductive and respiratory syndrome virus (PRRSV) readily crosses the maternal fetal interface (MFI) in third trimester, fetal resilience varies within litters. The aim of this study was to characterize PRRSV-2 concentration in MFI and fetuses at five time points after experimental inoculation of late gestation gilts and use this information to classify potentially resistant, resilient and susceptible fetuses. The secondary objective was to verify the relationship between PRRS viral load and intrauterine growth retardation (IUGR)...
December 5, 2018: Virus Research
Jan Adamowicz, Shane Van Breda, Dominik Tyloch, Marta Pokrywczynska, Tomasz Drewa
In reconstructive urology, autologous tissues such as intestinal segments, skin, and oral mucosa are used. Due to their limitations, reconstructive urologists are waiting for a novel material, which would be suitable for urinary tract wall replacement. Human amniotic membrane (AM) is a naturally derived biomaterial with a capacity to support reepithelization and inhibit scar formation. AM has a potential to become a considerable asset for reconstructive urology, i.e., reconstruction of ureters, urinary bladder and urethrae...
December 6, 2018: Expert Opinion on Biological Therapy
Daniel J Oh, Alexander L Pleet, Judy L Chen, Julie B Goldman, Amy Lin, Maria S Cortina
Purpose: We describe a rare case of a complex choristoma appearing as a salmon-patch lesion in the nasal conjunctiva. While benign, complex choristomas are grossly indistinguishable from malignant lesions, and an excisional biopsy is warranted to confirm the diagnosis. Observations: A 31-year-old man presented with an elevated glistening pink mass on the conjunctiva. An excisional biopsy with a "no-touch" technique was performed, followed by placement of an amniotic membrane graft without postoperative complications...
March 2019: American Journal of Ophthalmology Case Reports
Asmita Banerjee, Andrea Lindenmair, Ralf Steinborn, Sergiu Dan Dumitrescu, Simone Hennerbichler, Andrey V Kozlov, Heinz Redl, Susanne Wolbank, Adelheid Weidinger
The human amniotic membrane (hAM) has been used for tissue regeneration for over a century. In vivo ( in utero ), cells of the hAM are exposed to low oxygen tension (1-4% oxygen), while the hAM is usually cultured in atmospheric, meaning high, oxygen tension (20% oxygen). We tested the influence of oxygen tensions on mitochondrial and inflammatory parameters of human amniotic mesenchymal stromal cells (hAMSCs). Freshly isolated hAMSCs were incubated for 4 days at 5% and 20% oxygen. We found 20% oxygen to strongly increase mitochondrial oxidative phosphorylation, especially in placental amniotic cells...
2018: Stem Cells International
Giorgio Vescovo, Chiara Castellani, Marny Fedrigo, Grazia Maria Virzì, Giovanni Maria Vescovo, Regina Tavano, Michela Pozzobon, Annalisa Angelini
INTRODUCTION: We investigated the effects of human amniotic fluid stem cells (hAFS) and rat adipose tissue stromal vascular fraction GFP-positive cells (rSVC-GFP) in a model of cardio-renal syndrome type II (CRSII). METHODS AND RESULTS: RHF was induced by monocrotaline (MCT) in 28 Sprague-Dawley rats. Three weeks later, four million hAFS or rSVC-GFP cells were injected via tail vein. BNP, sCreatinine, kidney and heart NGAL and MMP9, sCytokines, kidney and heart apoptosis and cells (Cs) engraftment were evaluated...
January 15, 2019: International Journal of Cardiology
Marie Fukutake, Daigo Ochiai, Hirotaka Masuda, Yushi Abe, Yu Sato, Toshimitsu Otani, Shigeki Sakai, Noriko Aramaki-Hattori, Masayuki Shimoda, Tadashi Matsumoto, Kei Miyakoshi, Yae Kanai, Kazuo Kishi, Mamoru Tanaka
Adult wound healing can result in fibrotic scarring (FS) characterized by excess expression of myofibroblasts and increased type I/type III collagen expression. In contrast, fetal wound healing results in complete regeneration without FS, and the mechanism remains unclear. Amniotic fluid cells could contribute to scar-free wound healing, but the effects of human amniotic fluid cells are not well characterized. Here, we determined the effect of human amniotic fluid stem cells (hAFS) on FS during wound healing...
December 1, 2018: Human Cell
Christie M Buchovecky, Odelia Nahum, Brynn Levy
When biopsying a fetal tissue like chorionic villi or amniotic fluid, there is a chance of getting some maternal material that could contaminate the fetal specimen and might lead to a misdiagnosis. Thus, all prenatal samples should be subjected to testing for maternal cell contamination. This is done using quantitative fluorescent PCR (QF-PCR) of short tandem repeat (STR) markers.
2019: Methods in Molecular Biology
Odelia Nahum, Amanda Thomas, Brynn Levy
A prenatal noninvasive genetic screening test that yields a positive result typically warrants further direct assessment of fetal DNA following an invasive procedure. The precious nature of these invasively acquired samples, combined with the time sensitive nature with which results should be reported, demands that the methodologies used for analysis be quick, efficient, and dependable.Prenatal diagnosis has been performed using DNA extracted from amniotic fluid and chorionic villi for several decades, and more recently methodologies have been developed to extract cell free fetal DNA from amniotic fluid...
2019: Methods in Molecular Biology
Joana Witt, Maria Borrelli, Sonja Mertsch, Gerd Geerling, Kristina Spaniol, Stefan Schrader
Conjunctival repair is a crucial part of ocular surface reconstruction. Due to limitations of currently used substitute tissues, there is a need for the development of new scaffolds of consistent quality for conjunctival reconstruction. This study explored the biocompatibility and surgical usability of plastic compressed collagen (PCC) as an alternative conjunctival substitute. PCC gels were produced by polymerization of collagen type I followed by plastic compression. Conjunctival defects were trepanned in rabbits and grafted using PCC or human amniotic membrane (AM) or remained ungrafted...
December 1, 2018: Tissue Engineering. Part A
Xin Gu, Shi Hua, Matz Ethan, Liren Zhong, Ting Long, Cara Clouse, Wei Li, Dong Chen, HyunChul Chung, Sean Murphy, James Yoo, Guiting Lin, Tom Lu, Anthony Atala, Jackson John, Yuanyuan Zhang
OBJECTIVE: To determine the long-term therapeutic effect among three types of human cell types on erectile function recovery in a rodent model of dual neurovascular injured erectile dysfunction (NVED). MATERIALS AND METHODS: A dual NVED model was established in athymic rats by crushing the bilateral cavernous nerves and ligations of the bilateral internal pudendal nerve-vessel bundles. At the time of defect creation, three different types of human cell populations (2...
November 30, 2018: BJU International
Shulin Li, Yuan Zhao, Zhuojun Wang, Jia Wang, Caixia Liu, Dong Sun
Amniotic fluid-derived stem cells (AFSCs), which exhibit both embryonic and mesenchymal stem cell characteristics, have been shown to mitigate the degree of renal interstitial fibrosis. The aim of the present study was to determine whether transplantation of glial cell line-derived neurotrophic factor (GDNF)-modified AFSCs is more useful than transplantation of unmodified AFSCs for the treatment of renal interstitial fibrosis. Mice were randomly assigned to a sham-operation group (sham), a unilateral ureteral obstruction (UUO)-saline solution group (UUO), an AFSC transplantation group (AFSC) and a GDNF-modified AFSC transplantation group (GDNF-AFSC) and sacrificed at days 3 and 7 post-surgery (six in each group)...
November 30, 2018: Cell Transplantation
Sandeep Agrawal, Aditi Bhattacharya, Janvie Manhas, Yatin Kholakiya, Nupur Khera, Ajoy Roychoudhury, Sudip Sen
OBJECTIVE: Autologous cultured explants of human oral mucosal epithelial cells (OMEC) are a potential therapeutic modality in patients of bilateral ocular surface disease (OSD) with incapacitating dry eye. Mycophenolate mofetil (MMF) has been found to upregulate the mucin production in conjunctival goblet cells in vitro. The aim of this study was to evaluate the effects of MMF on mucin expression in primary cultures of OMEC. METHODS: With informed consent, oral mucosal epithelial tissue samples were obtained from patients undergoing oral surgery for non-malignant conditions...
December 20, 2018: Tokai Journal of Experimental and Clinical Medicine
Assem A Sultan, Linsen T Samuel, Alexander Roth, Bilal Mahmood, Nipun Sodhi, Michael A Mont
INTRODUCTION: Placental and amniotic membrane-based tissues have gained widespread popularity for their ability to promote healing and tissue regeneration and have manifested multiple applications in wound care, burn treatment, and management of various ocular conditions. Recently, there have been multiple studies that investigated the nonoperative uses of placental tissue-based products in orthopaedic sports injuries. However, there is a relative paucity of studies that have attempted to evaluate their adjuvant operative uses...
November 23, 2018: Surgical Technology International
Robert A Duerr, Jakob Ackermann, Andreas H Gomoll
In orthopedic sports medicine, amniotic-derived products have demonstrated promising preclinical and early clinical results for the treatment of tendon/ligament injuries, cartilage defects, and osteoarthritis. The amniotic membrane is a metabolically active tissue that has demonstrated anti-inflammatory, antimicrobial, antifibrotic, and epithelialization-promoting features that make it uniquely suited for several clinical applications. Although the existing clinical literature is limited, there are several ongoing clinical trials aiming to elucidate the specific applications and benefits of these products...
January 2019: Clinics in Sports Medicine
Natalia M Tulina, Amy G Brown, Guillermo O Barila, Michal A Elovitz
BACKGROUND: Exposure to intrauterine inflammation during pregnancy is linked to brain injury and neurobehavioral disorders in affected children. Innate immunity, specifically Toll-like receptor (TLR) signaling pathways are present throughout the reproductive tract as well as in the placenta, fetal membranes, and fetus. The TLR pathways are mechanistically involved in host responses to foreign pathogens and may lead to brain injury associated with prenatal inflammation. OBJECTIVE: We aimed to determine whether the activation of the TLR4 signaling pathway, in the mother and fetus, is critical to fetal brain injury in the setting of intrauterine inflammation...
November 21, 2018: Reproductive Sciences
Wan-Hua Cho, Ming-Tse Sung, Pei-Wen Lin, Hun-Ju Yu
RATIONALE: Limbal dermoids are choristomas known as congenital benign tumors found in abnormal locations. Despite the benign nature, enlarging limbal dermoids may cause visual abnormalities by cornea infiltration with fat component, visual axis invasion, gradually induced corneal astigmatism, and finally result in anisometropic amblyopia. Here we report a rare case of progressive, large pediatric corneal limbal dermoid in a newborn, managed with tissue glue-assisted monolayer amniotic membrane transplantation...
November 2018: Medicine (Baltimore)
Todd Jensen, Heather Wanczyk, Ishna Sharma, Adam Mitchell, Wael N Sayej, Christine Finck
BACKGROUND: Pediatric patients suffering from long gap esophageal defects or injuries are in desperate need of innovative treatment options. Our study demonstrates that two different cell sources can adhere to and proliferate on a retrievable synthetic scaffold. In feasibility testing of translational applicability, these cell seeded scaffolds were implanted into piglets and demonstrated esophageal regeneration. METHODS: Either porcine esophageal epithelial cells or porcine amniotic fluid was obtained and cultured in 3 dimensions on a polyurethane scaffold (Biostage)...
October 22, 2018: Journal of Pediatric Surgery
Ma Luisa Villalón, Ma De Los Ángeles Leal, José R Chávez, Eduardo M Santillán, Ismael Lares-Asseff, Verónica Loera, Laura Valencia, Blanca Camacho, Brenda Alvarado, Vilma Cervantes, Leslie Patrón, Horacio Almanza
BACKGROUND: The dermoid cyst considered a cystic teratoma derived from embryonic germinal epithelium is a slow-growing benign tumour. Dermoid cysts may occur in the orbital and periorbital region in paediatric patients and are often recurrent. The surgical approach depends upon the site of the lesion, superficial or deep. To our knowledge, this is the first described case of a patient with resection of dermoid cyst treated with human amniotic membrane implant and topical application of 0...
November 14, 2018: BMC Surgery
Taylor M Southworth, Neal B Naveen, Tracy M Tauro, Natalie L Leong, Brian J Cole
With average life expectancy and the rising prevalence of obesity, osteoarthritis (OA) is creating an increasingly large financial and physical burden on the U.S. population today. As the body ages and experiences trauma, articular cartilage surfaces in joints are gradually worn away, leading to OA. Traditionally, treatment options have included lifestyle modifications, pain management, and corticosteroid injections, with joint replacement reserved for those who have exhausted nonsurgical measures. More recently, hyaluronic acid, micronized dehydrated human amniotic/chorionic membrane tissue, and platelet-rich plasma (PRP) injections have started to gain traction...
November 13, 2018: Journal of Knee Surgery
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