Iva Guberovic, Sarah Hurtado-Bagès, Ciro Rivera-Casas, Gunnar Knobloch, Roberto Malinverni, Vanesa Valero, Michelle M Leger, Jesús García, Jerome Basquin, Marta Gómez de Cedrón, Marta Frigolé-Vivas, Manjinder S Cheema, Ainhoa Pérez, Juan Ausió, Ana Ramírez de Molina, Xavier Salvatella, Iñaki Ruiz-Trillo, Jose M Eirin-Lopez, Andreas G Ladurner, Marcus Buschbeck
NAD metabolism is essential for all forms of life. Compartmental regulation of NAD+ consumption, especially between the nucleus and the mitochondria, is required for energy homeostasis. However, how compartmental regulation evolved remains unclear. In the present study, we investigated the evolution of the macrodomain-containing histone variant macroH2A1.1, an integral chromatin component that limits nuclear NAD+ consumption by inhibiting poly(ADP-ribose) polymerase 1 in vertebrate cells. We found that macroH2A originated in premetazoan protists...
December 2021: Nature Structural & Molecular Biology