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JOURNAL ARTICLE
Linnea K M Blomgren, Melanie Huber, Sabrina R Mackinnon, Céline Bürer, Arnaud Baslé, Wyatt W Yue, D Sean Froese, Thomas J McCorvie
5,10-methylenetetrahydrofolate reductase (MTHFR) commits folate-derived one-carbon units to generate the methyl-donor S-adenosyl-L-methionine (SAM). Eukaryotic MTHFR appends to the well-conserved catalytic domain (CD) a unique regulatory domain (RD) that confers feedback inhibition by SAM. Here we determine the cryo-electron microscopy structures of human MTHFR bound to SAM and its demethylated product S-adenosyl-L-homocysteine (SAH). In the active state, with the RD bound to a single SAH, the CD is flexible and exposes its active site for catalysis...
April 15, 2024: Nature Communications
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JOURNAL ARTICLE
Sreelakshmi Kotha, Giovanni Tritto, Eleni Theocharidou, Terry Wong, Bo Wang, Philip Berry
In 2017 the Royal College of Physicians launched a voluntary accreditation process supported by British Association for the Study of the Liver (BASL) and the British Society of Gastroenterologists (BSG) to improve the quality and consistency of liver services across the UK and Ireland. This article describes the approach that we took and the challenges that we met on the way to achieving accreditation.
May 2023: Clinical Medicine: Journal of the Royal College of Physicians of London
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JOURNAL ARTICLE
Michael L Pretterklieber
As the title indicates, this article deals with the origins of anatomic terminology and its development up to the present day. The first attempt to name anatomical structures in animals and humans date back to Alkmaion, i.e. to the fifth century BC. Further work has been done at the same time by the Hippocratics and about 100 years later by Aristotle. As the Alexandrians Erasistratos and Herophilos first in history dissected human bodies, they expanded the anatomical terms. Until Celsus (around Christ's birth) and even later on, anatomical terminology was almost exclusively based on the Greek language...
April 5, 2024: Anatomical Science International
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JOURNAL ARTICLE
Thomas J McCorvie, Douglas Adamoski, Raquel A C Machado, Jiazhi Tang, Henry J Bailey, Douglas S M Ferreira, Claire Strain-Damerell, Arnaud Baslé, Andre L B Ambrosio, Sandra M G Dias, Wyatt W Yue
Cystathionine beta-synthase (CBS) is an essential metabolic enzyme across all domains of life for the production of glutathione, cysteine, and hydrogen sulfide. Appended to the conserved catalytic domain of human CBS is a regulatory domain that modulates activity by S-adenosyl-L-methionine (SAM) and promotes oligomerisation. Here we show using cryo-electron microscopy that full-length human CBS in the basal and SAM-bound activated states polymerises as filaments mediated by a conserved regulatory domain loop...
April 4, 2024: Nature Communications
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JOURNAL ARTICLE
Agustín Morales, Caroline Gonçalves, Alix Sournia-Saquet, Laure Vendier, Agustí Lledós, Olivier Baslé, Sébastien Bontemps
The carbon dioxide radical anion [CO2 ˙- ] is a highly reactive species of fundamental and synthetic interest. However, the direct one-electron reduction of CO2 to generate [CO2 ˙- ] occurs at very negative reduction potentials, which is often a limiting factor for applications. Here, we show that NHC-CO2 -BR3 species - generated from the Frustrated Lewis Pair (FLP)-type activation of CO2 by N-heterocyclic carbenes (NHCs) and boranes (BR3 ) - undergo single electron reduction at a less negative potential than free CO2 ...
February 28, 2024: Chemical Science
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JOURNAL ARTICLE
Karla Helena-Bueno, Mariia Yu Rybak, Chinenye L Ekemezie, Rudi Sullivan, Charlotte R Brown, Charlotte Dingwall, Arnaud Baslé, Claudia Schneider, James P R Connolly, James N Blaza, Bálint Csörgő, Patrick J Moynihan, Matthieu G Gagnon, Chris H Hill, Sergey V Melnikov
To conserve energy during starvation and stress, many organisms use hibernation factor proteins to inhibit protein synthesis and protect their ribosomes from damage1,2 . In bacteria, two families of hibernation factors have been described, but the low conservation of these proteins and the huge diversity of species, habitats and environmental stressors have confounded their discovery3-6 . Here, by combining cryogenic electron microscopy, genetics and biochemistry, we identify Balon, a new hibernation factor in the cold-adapted bacterium Psychrobacter urativorans...
February 14, 2024: Nature
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JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
January 5, 2024: Gut
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JOURNAL ARTICLE
Michelle N Meyer, John Basl, David Choffnes, Christo Wilson, David M J Lazer
No abstract text is available yet for this article.
August 2023: Nature computational science
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JOURNAL ARTICLE
Wenhao Li, David Sheridan, Stuart McPherson, William Alazawi
BACKGROUND & AIMS: Non-alcoholic fatty liver disease (NAFLD) is associated with liver and cardiovascular morbidity and mortality. Recently published NAFLD Quality Standards include 11 key performance indicators (KPIs) of good clinical care. This national study, endorsed by British Association for the Study of the Liver (BASL) and British Society of Gastroenterology (BSG), aimed to benchmark NAFLD care in UK hospitals against these KPIs. METHODS: This study included all new patients with NAFLD reviewed in the outpatient clinic in the months of March 2019 and March 2022...
December 2023: JHEP reports: innovation in hepatology
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JOURNAL ARTICLE
Joëlle Claves, Philip Chennell, Yoann Le Basle, Nina Krautwurst, Valérie Sautou
INTRODUCTION: Medical syringes are widely used in hospitals to store and administer drugs, and the contact time between the drugs and these syringes can vary from a few minutes to several weeks like for pharmaceutical preparations. The aim of this comparative study was to evaluate the potential sorption phenomena occurring between three drugs (paracetamol, diazepam and insulin aspart) and polypropylene syringes (PP) or syringes made of Cyclic Olefin Copolymer (COC). MATERIALS AND METHODS: 50 mL 3-part syringes made of either COC with crosslinked silicone on the barrel inner surface (COC-CLS) and a bromobutyl plunger seal, or PP lubricated with silicone oil (PP-SOL) with a polyisoprene plunger seal were used...
November 21, 2023: Pharmaceutical Research
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JOURNAL ARTICLE
Augustinas Silale, Yiling Zhu, Jerzy Witwinowski, Robert E Smith, Kahlan E Newman, Satya P Bhamidimarri, Arnaud Baslé, Syma Khalid, Christophe Beloin, Simonetta Gribaldo, Bert van den Berg
The outer membrane (OM) in diderm, or Gram-negative, bacteria must be tethered to peptidoglycan for mechanical stability and to maintain cell morphology. Most diderm phyla from the Terrabacteria group have recently been shown to lack well-characterised OM attachment systems, but instead have OmpM, which could represent an ancestral tethering system in bacteria. Here, we have determined the structure of the most abundant OmpM protein from Veillonella parvula (diderm Firmicutes) by single particle cryogenic electron microscopy...
November 6, 2023: Nature Communications
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JOURNAL ARTICLE
Marie Desclos-Theveniau, Hugo Abgrall, Vincent Bar, Françoise Basle, Dominique Basle, Marie-Joseph Dautel, Jacques Rubin, Franck Delanoue, Etienne Lefrançois, Anne Dautel, Anne-Laure Sérandour, Gwenvael Le Guicher, Hugues Colombel
STATEMENT OF PROBLEM: Gingival displacement is used in prosthodontics to obtain an accurate impression. However, randomized clinical trials to analyze the performance of different gingival displacement products are lacking. PURPOSE: The purpose of this prospective, comparative randomized clinical trial was to evaluate the clinical effectiveness of 3 gingival displacement techniques: Racegel cordless, Racegel with a cord, and Racestyptine with a cord. MATERIAL AND METHODS: A prospective, multicenter randomized, open label, 3-arm parallel group study was carried out in private dental practices...
October 31, 2023: Journal of Prosthetic Dentistry
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JOURNAL ARTICLE
José Fernando Huertas-Pérez, Quentin Baslé, Mathieu Dubois, Xanthippe Theurillat
An analytical method for determination of GC-amenable pesticides in complex matrices, was validated based on QuEChERS sample preparation and gas chromatography tandem mass spectrometry. Performance of the method was tested according to the EU SANTE guidelines for 172 pesticides belonging to different chemical classes, in three representative complex matrices. Three concentration levels were tested in order to establish the lowest limit of quantification possible. For some matrix/pesticide combination, careful selection of the quantification/confirmation transitions was key to avoid interferences...
October 13, 2023: Food Chemistry
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JOURNAL ARTICLE
Ani Paloyan, Armen Sargsyan, Mariam D Karapetyan, Artur Hambardzumyan, Sergei Kocharov, Henry Panosyan, Karine Dyukova, Marina Kinosyan, Anna Kreuger, Cecilia Piergentili, Will A Stanley, Karrera Y Djoko, Arnaud Baslé, Jon Marles-Wright, Garabed Antranikian
N-carbamoyl-β-alanine amidohydrolase (CβAA) constitutes one of the most important groups of industrially relevant enzymes used in the production of optically pure amino acids and derivatives. In this study, a CβAA-encoding gene from Rhizobium radiobacter strain MDC 8606 was cloned and overexpressed in Escherichia coli. The purified recombinant enzyme (RrCβAA) showed a specific activity of 14 U/mg using N-carbamoyl-β-alanine as a substrate with an optimum activity at 55°C and pH 8...
August 27, 2023: FEBS Journal
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JOURNAL ARTICLE
Eva-Sophie Wallner, Liam Dolan, Dominique C Bergmann
Cell polarity combined with asymmetric cell divisions (ACDs) generates cellular diversity. In the Arabidopsis stomatal lineage, a single cortical polarity domain marked by BASL orients ACDs and is segregated to the larger daughter to enforce cell fate. We discovered a second, oppositely positioned polarity domain defined by OCTOPUS-LIKE (OPL) proteins, which forms prior to ACD and is segregated to the smaller (meristemoid) daughter. Genetic and misexpression analyses show that OPLs promote meristemoid-amplifying divisions and delay stomatal fate progression...
August 17, 2023: Developmental Cell
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JOURNAL ARTICLE
Javier Abellon-Ruiz, Kalyanashis Jana, Augustinas Silale, Andrew M Frey, Arnaud Baslé, Matthias Trost, Ulrich Kleinekathöfer, Bert van den Berg
Vitamin B12 (cobalamin) is required for most human gut microbes, many of which are dependent on scavenging to obtain this vitamin. Since bacterial densities in the gut are extremely high, competition for this keystone micronutrient is severe. Contrasting with Enterobacteria, members of the dominant genus Bacteroides often encode several BtuB vitamin B12 outer membrane transporters together with a conserved array of surface-exposed B12 -binding lipoproteins. Here we show that the BtuB transporters from Bacteroides thetaiotaomicron form stable, pedal bin-like complexes with surface-exposed BtuG lipoprotein lids, which bind B12 with high affinities...
August 5, 2023: Nature Communications
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JOURNAL ARTICLE
José Fernando Huertas-Pérez, Pascal Mottier, Erik Konings, Quentin Baslé, Shi Ying Tan, Monika Kopeć-Durska, Patrycja Zawada, Ashley Griffin, María Guadalupe Sánchez-Calderón, Juan Pablo Silva-Robledo, Lisette Rubio
BACKGROUND: Chlorate is an effective herbicide, but also a by-product of chlorinating agents used to disinfect water, one of the reasons why it is regularly found in food. Perchlorate is a ubiquitous contaminant, which is naturally occurring in the environment but also released from anthropogenic sources such as the industrial use of certain natural fertilizers. Chlorate affects the hematological system, and perchlorate the thyroid. OBJECTIVE: Implement and validate a simple and robust analytical method for the accurate determination of chlorate and perchlorate in baby food, infant and adult formulas, and ingredients thereof, which is suited for its application in routine environments where a broad variety of food commodities must be analyzed simultaneously...
July 18, 2023: Journal of AOAC International
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JOURNAL ARTICLE
Joshua B R White, Augustinas Silale, Matthew Feasey, Tiaan Heunis, Yiling Zhu, Hong Zheng, Akshada Gajbhiye, Susan Firbank, Arnaud Baslé, Matthias Trost, David N Bolam, Bert van den Berg, Neil A Ranson
Bacteroidetes are abundant members of the human microbiota, utilizing a myriad of diet- and host-derived glycans in the distal gut1 . Glycan uptake across the bacterial outer membrane of these bacteria is mediated by SusCD protein complexes, comprising a membrane-embedded barrel and a lipoprotein lid, which is thought to open and close to facilitate substrate binding and transport. However, surface-exposed glycan-binding proteins and glycoside hydrolases also play critical roles in the capture, processing and transport of large glycan chains...
June 2023: Nature
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JOURNAL ARTICLE
Ben Ashley, Arnaud Baslé, Mariyah Sajjad, Ahmed El Ashram, Panayiota Kelis, Jon Marles-Wright, Dominic J Campopiano
We report a chemo-biocatalytic cascade for the synthesis of substituted pyrroles, driven by the action of an irreversible, thermostable, pyridoxal 5'-phosphate (PLP)-dependent, C-C bond-forming biocatalyst ( Th AOS). The Th AOS catalyzes the Claisen-like condensation between various amino acids and acyl-CoA substrates to generate a range of α-aminoketones. These products are reacted with β-keto esters in an irreversible Knorr pyrrole reaction. The determination of the 1.6 Å resolution crystal structure of the PLP-bound form of Th AOS lays the foundation for future engineering and directed evolution...
May 29, 2023: ACS Sustainable Chemistry & Engineering
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JOURNAL ARTICLE
Jon Agirre, Mihaela Atanasova, Haroldas Bagdonas, Charles B Ballard, Arnaud Baslé, James Beilsten-Edmands, Rafael J Borges, David G Brown, J Javier Burgos-Mármol, John M Berrisford, Paul S Bond, Iracema Caballero, Lucrezia Catapano, Grzegorz Chojnowski, Atlanta G Cook, Kevin D Cowtan, Tristan I Croll, Judit É Debreczeni, Nicholas E Devenish, Eleanor J Dodson, Tarik R Drevon, Paul Emsley, Gwyndaf Evans, Phil R Evans, Maria Fando, James Foadi, Luis Fuentes-Montero, Elspeth F Garman, Markus Gerstel, Richard J Gildea, Kaushik Hatti, Maarten L Hekkelman, Philipp Heuser, Soon Wen Hoh, Michael A Hough, Huw T Jenkins, Elisabet Jiménez, Robbie P Joosten, Ronan M Keegan, Nicholas Keep, Eugene B Krissinel, Petr Kolenko, Oleg Kovalevskiy, Victor S Lamzin, David M Lawson, Andrey A Lebedev, Andrew G W Leslie, Bernhard Lohkamp, Fei Long, Martin Malý, Airlie J McCoy, Stuart J McNicholas, Ana Medina, Claudia Millán, James W Murray, Garib N Murshudov, Robert A Nicholls, Martin E M Noble, Robert Oeffner, Navraj S Pannu, James M Parkhurst, Nicholas Pearce, Joana Pereira, Anastassis Perrakis, Harold R Powell, Randy J Read, Daniel J Rigden, William Rochira, Massimo Sammito, Filomeno Sánchez Rodríguez, George M Sheldrick, Kathryn L Shelley, Felix Simkovic, Adam J Simpkin, Pavol Skubak, Egor Sobolev, Roberto A Steiner, Kyle Stevenson, Ivo Tews, Jens M H Thomas, Andrea Thorn, Josep Triviño Valls, Ville Uski, Isabel Usón, Alexei Vagin, Sameer Velankar, Melanie Vollmar, Helen Walden, David Waterman, Keith S Wilson, Martyn D Winn, Graeme Winter, Marcin Wojdyr, Keitaro Yamashita
The Collaborative Computational Project No. 4 (CCP4) is a UK-led international collective with a mission to develop, test, distribute and promote software for macromolecular crystallography. The CCP4 suite is a multiplatform collection of programs brought together by familiar execution routines, a set of common libraries and graphical interfaces. The CCP4 suite has experienced several considerable changes since its last reference article, involving new infrastructure, original programs and graphical interfaces...
June 1, 2023: Acta Crystallographica. Section D, Structural Biology
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