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Diana M Elizondo, Temesgen E Andargie, Naomi L Haddock, Ricardo L Louzada da Silva, Tatiana Rodrigues de Moura, Michael W Lipscomb
Allograft Inflammatory Factor-1 (AIF1) is a cytoplasmic scaffold protein that contains Ca2+ binding EF-hand and PDZ interaction domains important for mediating intracellular signaling complexes in immune cells. The protein plays a dominant role in both macrophage- and dendritic cell (DC)-mediated inflammatory responses. This study now reports that AIF1 expression in DC is important in directing CD8+ T cell effector responses. Silencing AIF1 expression in murine CD11c+ DC suppressed antigen-specific CD8+ T cell activation, marked by reduced CXCR3, IFNγ and Granzyme B expression, and restrained proliferation...
December 4, 2018: Journal of Leukocyte Biology
Katarina Elez, Alexandre M J J Bonvin, Anna Vangone
BACKGROUND: Study of macromolecular assemblies is fundamental to understand functions in cells. X-ray crystallography is the most common technique to solve their 3D structure at atomic resolution. In a crystal, however, both biologically-relevant interfaces and non-specific interfaces resulting from crystallographic packing are observed. Due to the complexity of the biological assemblies currently tackled, classifying those interfaces, i.e. distinguishing biological from crystal lattice interfaces, is not trivial and often prone to errors...
November 30, 2018: BMC Bioinformatics
Nicholas T Haddock, Sumeet T Teotia
No abstract text is available yet for this article.
December 2018: Plastic and Reconstructive Surgery
Jose L Garrido, Joseph Presscott, Mario Calvo, Felipe Bravo, Raymond Alvarez, Alexis Salas, Raul Riquelme, Maria L Rioseco, Brandi N Williamson, Elaine Haddock, Heinz Feldmann, Maria I Barria
Andes hantavirus (ANDV) is an etiologic agent of hantavirus cardiopulmonary syndrome (HCPS), a severe disease characterized by fever, headache, and gastrointestinal symptoms that may progress to hypotension, pulmonary failure, and cardiac shock that results in a 25 to 40% case-fatality rate. Currently, there is no specific treatment or vaccine; however, several studies have shown that the generation of neutralizing antibody (Ab) responses strongly correlates with survival from HCPS in humans. In this study, we screened 27 ANDV convalescent HCPS patient sera for their capacity to bind and neutralize ANDV in vitro...
November 21, 2018: Science Translational Medicine
Oleg Klykov, Barbara Steigenberger, Sibel Pektaş, Domenico Fasci, Albert J R Heck, Richard A Scheltema
Cross-linking mass spectrometry (XL-MS) has received considerable interest, owing to its potential to investigate protein-protein interactions (PPIs) in an unbiased fashion in complex protein mixtures. Recent developments have enabled the detection of thousands of PPIs from a single experiment. A unique strength of XL-MS, in comparison with other methods for determining PPIs, is that it provides direct spatial information for the detected interactions. This is accomplished by the use of bifunctional cross-linking molecules that link two amino acids in close proximity with a covalent bond...
December 2018: Nature Protocols
Haojie Huang, Jiaxiang Chen, Lisi Peng, Yao Yao, Defeng Deng, Yang Zhang, Yan Liu, Huamin Wang, Zhaoshen Li, Yan Bi, Ashley N Haddock, Xianbao Zhan, Weiqin Lu, Craig D Logsdon, Baoan Ji
INTRODUCTION: Replacement of the exocrine parenchyma by fibrous tissue is a main characteristic of chronic pancreatitis. Understanding the mechanisms of pancreatic fibrogenesis is critical for the development of preventive and therapeutic interventions. Cyclooxygenase-2 (COX-2), a rate-limiting enzyme for prostaglandin synthesis, is expressed in patients with chronic pancreatitis. However, it is unknown whether COX-2 can cause chronic pancreatitis. AIMS AND METHODS: To investigate the roles of pancreatic acinar COX-2 in fibrogenesis and the development of chronic pancreatitis, COX-2 was ectopically expressed specifically in pancreatic acinar cells in transgenic mice...
November 15, 2018: American Journal of Physiology. Gastrointestinal and Liver Physiology
Nimesh A Patel, Parth Shah, Nicolas A Yannuzzi, Zubair Ansari, Jill S Zaveri, Nidhi Relhan, Basil K Williams, Ajay E Kuriyan, Christopher R Henry, Jayanth Sridhar, Luis Haddock, Jorge A Fortun, Thomas A Albini, Janet L Davis, Harry W Flynn
Purpose: To report the visual outcomes and complications of scleral fixated intraocular lenses (IOLs) using Gore-Tex suture. Methods: The current study is a retrospective noncomparative case series including patients who underwent scleral fixation of IOL (Akreos AO60) using Gore-Tex suture from August 2015 to March 2017 at a university teaching center. Primary outcome measures were visual acuity and complications at last follow-up. Results: The current study included 49 eyes of 48 patients...
2018: Clinical Ophthalmology
David Boulée, Sarathi Kalra, Alison Haddock, T David Johnson, W Frank Peacock
BACKGROUND: Stethoscopes can be microorganism reservoirs. The US Centers for Disease Control and Prevention (CDC) has published medical equipment disinfection guidelines to minimize infection transmission risk, but studies of guideline adherence have been predominately survey based, with little direct observation of disinfection practices. METHODS: We performed an observational, cross-sectional, anonymous study of patient-provider interactions, assessing practitioners' frequency and methods of stethoscope and hand disinfection practices...
November 2, 2018: American Journal of Infection Control
Sandra Bucci, Rohan Morris, Katherine Berry, Natalie Berry, Gillian Haddock, Christine Barrowclough, Shôn Lewis, Dawn Edge
BACKGROUND: Digital technology has the potential to improve outcomes for people with psychosis. However, to date, research has largely ignored service user views on digital health interventions (DHIs). OBJECTIVE: The objective of our study was to explore early psychosis service users' subjective views on DHIs. METHODS: Framework analysis was undertaken with data obtained from 21 semistructured interviews with people registered with early intervention for psychosis services...
October 31, 2018: JMIR Mental Health
Jonathan F Russell, Nathan L Scott, Luis J Haddock, Alexander M Eaton, Harry W Flynn
Purpose: To report two cases of central retinal artery occlusion (CRAO) associated with vitreoretinal surgery. Observations: Two patients underwent vitreoretinal surgery and were diagnosed with CRAO on postoperative day one. Both had received retrobulbar anesthetic blocks, followed by pars plana vitrectomy in one patient and scleral buckling in the other patient. Best-corrected visual acuity at last follow-up was 20/40 and 20/400. Conclusions/Importance: CRAO is a rare but serious adverse event after vitreoretinal surgery...
December 2018: American Journal of Ophthalmology Case Reports
Sumeet S Teotia, Min-Jeong Cho, Nicholas T Haddock
Background: Over the years, the choice of recipient vessels for free flap autologous breast reconstruction has shifted from the thoracodorsal to the internal mammary vessels due to ease of flap inset and predictability of anatomy. However, thoracodorsal vessels are still great recipient vessels, and can be useful, especially in the previously failed or staged autologous breast reconstruction. In this study, we present our experience using thoracodorsal or serratus vessels for profunda artery perforator flaps...
September 2018: Plastic and Reconstructive Surgery. Global Open
Andrew A Gassman, Judy Pan, Edwin Acevedo, Nicholas Haddock, Sumeet Teotia
The superficial inferior epigastric artery (SIEA) flap and the deep inferior epigastric perforator (DIEP) flap have been increasingly adopted for breast reconstruction; however, each have its own set of advantages and disadvantages. In the select subset of patients that cannot tolerate minimal abdominal fasciotomy that occurs with DIEP harvest and do not have adequate pedicle length that often occurs after SIEA harvest, we suggest another option for abdominally-based free flap breast reconstruction. Here, we describe the formation of a composite perforator based on the pedicle of the retro-rectus deep inferior epigastric vessels and the superficial inferior epigastric vessels, known as a superficial and deep inferior epigastric artery (SADIE) flap, which allows for a more compatible anastomotic size match than the SIEA and elongation of the vascular pedicle with minimal dissection of the anterior rectus fascia...
October 2018: Microsurgery
Yvonne F Awenat, Sarah Peters, Patricia A Gooding, Daniel Pratt, Emma Shaw-Núñez, Kamelia Harris, Gillian Haddock
BACKGROUND: Suicide is a global problem and suicidal behavior is common in acute psychiatric wards. Inpatient suicides regularly occur with 10.4/100,000 such deaths recorded in the UK in 2016. Inpatient suicides are potentially the most avoidable of all suicides as inpatients have 24-h staff contact. Current inpatient treatment prioritizes maintenance of physical safety by observation, medication and general supportive measures, however efficacious and effective specific treatments are lacking...
October 16, 2018: BMC Psychiatry
Maryam Ghodrati Siahmazgi, Mohammad Ali Nasiri Khalili, Fathollah Ahmadpour, Sirus Khodadadi, Mehdi Zeinoddini
BACKGROUND: The negative effect of chemotherapy and radiotherapy on normal tissues as well as the high expense and length of these treatments have recently attracted researchers to the new methods that specifically affect cancerous tissues and have lower damage to normal tissues. One of these methods is the use of intelligent recombinant fusion toxin. The fusion toxin DT-GCSF, which consists of linked diphtheria toxin (DT) and granulocyte colony stimulate factor (GCSF), was first studied by Chadwick et al...
October 12, 2018: Current Computer-aided Drug Design
Diana M Elizondo, Temesgen E Andargie, Naomi L Haddock, Thomas A Boddie, Michael W Lipscomb
Phagocytosis, macropinocytosis and antigen presentation by dendritic cells (DC) requires reorganization of the actin cytoskeleton. Drebrin (Dbn1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. However, its role in DC phagocytosis and antigen presentation is unknown. These studies now report that silencing of Dbn1 in DC resulted in restrained cell surface display of receptors, most notably MHC class I and II and costimulatory molecules...
October 14, 2018: Immunology
P R Pugh, C W Dunn, S H D Haddock
A new species of calycophoran siphonophore, Tottonophyes enigmatica gen. nov, sp. nov., is described. It has a unique combination of traits, some shared with prayomorphs (including two rounded nectophores) and some with clausophyid diphyomorphs (the nectophores are dissimilar, with one slightly larger and slightly to the anterior of the other, and both possess a somatocyst). Molecular phylogenetic analyses indicate that the new species is the sister group to all other diphyomorphs. A new family, Tottonophyidae, is established for it...
May 1, 2018: Zootaxa
Richard R Suminski, Walker S C Poston, R Sue Day, Nattinee Jitnarin, C Keith Haddock, Sara A Jahnke, Gregory M Dominick
OBJECTIVES: To establish the extent of steady state hypohydration among firefighters and examine factors associated with their hydration status. METHODS: Data on 450 career firefighters from 11 randomly selected fire departments had their body composition, total body water (TBW), and hypohydration status assessed using bioelectrical impedance. Measured height and weight were used to determine body mass index (BMI) and weight classification. RESULTS: The hypohydration rate was 17% and 94% of hypohydrated firefighters were obese...
October 10, 2018: Journal of Occupational and Environmental Medicine
Laith A Jawad, Tom J Hansen, Sonnich Meier, Jon Egil Skjaeraasen, Per Gunnar Fjelldal
This study describes radiological malformations in the vertebral column of adult sexually mature wild haddock, Melanogrammus aeglefinus collected from Masfjorden, Western Norway, in 2014 and 2015. There were seven deformed fish (33% of sampled fish), five with mild deformities and two with severe deformities. The deformity types discovered were lordosis, vertebra body asymmetry, compression, fusion (ankylosis), remodelling, and haemal spine hyperostosis. The two severely deformed fish were as follows: (a) one specimen with 29 deformed vertebrae within the region vertebrae nos...
October 7, 2018: Journal of Fish Diseases
David M Routman, Ryan K Funk, Bradley J Stish, Lance A Mynderse, Torrence M Wilson, Robert McLaren, W Scott Harmsen, Kristin Mara, Christopher L Deufel, Keith M Furutani, Michael G Haddock, Thomas M Pisansky, C Richard Choo, Brian J Davis
PURPOSE: To report outcomes of patients undergoing low-dose-rate (LDR) brachytherapy and investigate factors associated with biochemical failure and survival. METHODS: Consecutive patients undergoing LDR with I-125 at our institution between 1998 through 2013 for primary intact prostate cancer were examined. Those with low- and intermediate-risk disease receiving LDR with a minimum of 2 years followup and at least one post-LDR prostate-specific antigen (PSA) were included...
October 4, 2018: Brachytherapy
Sneha Rai, Pallavi Mohanty, Sonika Bhatnagar
PH domains mediate interactions involved in cell signaling, intracellular membrane transport regulation and cytoskeleton organization. Some PH domains bind phosphoinositides with different affinity and specificity. The two novel PLCη (1 and 2) possess an N-terminal PH domain (PHη1 and PHη2 respectively) that has been implicated in membrane association and induction of PLC activity. Understanding of the structure and dynamics is crucial for future modulation of lipid-protein interactions in PHη1, PHη2 and other PH domains...
August 29, 2018: Journal of Molecular Graphics & Modelling
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