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https://www.readbyqxmd.com/read/30030936/the-soluble-isoform-of-human-fc%C3%A9-ri-is-an-endogenous-inhibitor-of-ige-mediated-mast-cell-responses
#1
Sherezade Moñino-Romero, Lena Erkert, Klara Schmidthaler, Susanne C Diesner, Benjamin F Sallis, Luke Pennington, Theodore Jardetzky, Hans C Oettgen, Barbara Bohle, Edda Fiebiger, Zsolt Szépfalusi
BACKGROUND: The soluble isoform of FcɛRI, the high affinity IgE receptor (sFcεRI), is a protein of the IgE network with poorly defined functions. OBJECTIVE: Define cellular sources and signals that result in the production of human sFcεRI and study its in vivo functions. METHODS: FcεRI-transfected human cell lines (MelJuso), human monocyte-derived dendritic cells (moDCs), and murine bone marrow-derived mast cells (MC) were stimulated by FcεRI crosslinking and release of sFcεRI was analyzed (ELISA, Western Blot)...
July 21, 2018: Allergy
https://www.readbyqxmd.com/read/30009843/allograft-rejection-is-associated-with-the-development-of-functional-ige-specific-for-donor-mhc-antigens
#2
Andreas M Farkas, Ulrike Baranyi, Georg A Böhmig, Lukas Unger, Stefan Hopf, Markus Wahrmann, Heinz Regele, Benedikt Mahr, Christoph Schwarz, Karin Hock, Nina Pilat, Ivan Kristo, Jasmin Mraz, Christian Lupinek, Josef Thalhamer, Gregor Bond, Lorenz Kuessel, Elizabeth Wlodek, Jack Martin, Menna Clatworthy, Gavin Pettigrew, Rudolf Valenta, Thomas Wekerle
BACKGROUND: Donor-specific antibodies (DSA) of the IgG isotype are routinely measured for diagnostic purposes in renal transplant recipients and are associated with antibody-mediated rejection and long-term graft loss. OBJECTIVE: This study aimed to investigate if MHC-specific antibodies of the IgE isotype are induced during the rejection of allografts. METHODS: Anti-MHC/HLA IgE was measured in sera of mice grafted with skin or heart transplants from various donor strains and in sera of kidney transplant patients with high levels of HLA-IgG...
July 12, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29977237/phenotype-function-and-mobilization-of-6-sulfo-lacnac-expressing-monocytes-in-atopic-dermatitis
#3
Wojciech Baran, Stephanie Oehrl, Fareed Ahmad, Thomas Döbel, Christina Alt, Angelika Buske-Kirschbaum, Marc Schmitz, Knut Schäkel
Mononuclear phagocytes (MPs) are important immune regulatory cells in atopic dermatitis (AD). We previously identified 6-sulfo LacNAc-expressing monocytes (slanMo) as TNF-α- and IL-23-producing cells in psoriatic skin lesions and as inducers of IFN-γ-, IL-17-, and IL-22-producing T cells. These cytokines are also upregulated in AD and normalize with treatment, as recently shown for dupilumab-treated patients. We here asked for the role of slanMo in AD. Increased numbers of slanMo were found in AD skin lesions...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29961151/mast-cells-participate-in-allograft-rejection-can-il-37-play-an-inhibitory-role
#4
REVIEW
Pio Conti, Alessandro Caraffa, Gianpaolo Ronconi, Spiros K Kritas, Filiberto Mastrangelo, Lucia Tettamanti, Ilias Frydas, Theoharis C Theoharides
OBJECTIVE: The aim of this study was to evaluate the role of mast cells (MCs) in allograft rejection, eventually inhibited by IL-37. Immune cells including MCs participate in allograft rejection by generating IL-1, IL-33, TNF and other cytokines. METHODS: We evaluated allograft rejection on the experience of our experimental data and using the relevant literature. RESULTS: MCs are involved in initiation and regulation of innate and adaptive immune responses-pathways...
June 30, 2018: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/29859965/auto-antibodies-to-ige-and-fceri-and-the-natural-variability-of-syk-expression-in-basophils
#5
Donald MacGlashan
BACKGROUND: Secretion from human basophils and mast cells requires the activity of SYK but expression of SYK is highly variable in the general population and this variability predicts the magnitude of IgE-mediated secretion. One known mechanism of modulating SYK expression in human basophils is aggregation of FceRI. OBJECTIVE: This study examines the possibility that functional auto-antibodies are present in a wide variety of subjects and in particular, subjects whose basophils poorly express SYK...
May 31, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29662845/suppressive-effects-of-vaccinium-angustifolium-root-extract-via-down-regulation-of-activation-of-syk-lyn-and-nf-%C3%AE%C2%BAb-in-fc%C3%A9-ri-mediated-allergic-reactions
#6
Sun-Yup Shim
Vaccinium angustifolium , reported as the lowbush blueberry, has a rich polyphenolic content with which biological activities have been closely associated. In this study, the effects of V. angustifolium root extract (VAE) on the anti-FcɛRI α chain antibody (CRA-1)-induced FcɛRI-mediated signaling factors, protein tyrosine kinases (PTK), Lyn, Syk, and nuclear factor kappa-B cells (NF-κB) in KU812F cells were investigated. The total phenolic content of VAE was found to be 170±1.9 mg gallic acid equivalents/g...
March 2018: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/29365287/platelets-play-a-central-role-in-sensitization-to-allergen
#7
Richard T Amison, Simon J Cleary, Yanira Riffo-Vasquez, Maidda Bajwa, Clive P Page, Simon C Pitchford
Platelet activation occurs in patients with allergic inflammation, and platelets can be activated directly by allergen via an IgE-dependent process. Platelets have been shown to activate APCs such as CD11c+ dendritic cells in vitro. Although CD11c+ dendritic cells are a requisite for allergen sensitization, the role of platelets in this process is unknown. In this study, we investigated whether platelets were necessary for allergen sensitization. Balb/c mice sensitized to ovalbumin were exposed to subsequent aerosolized allergen (ovalbumin challenge)...
July 2018: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/29336501/uncontrolled-asthmatics-have-increased-fceri-and-tgf-%C3%AE-positive-mc-tc-mast-cells-and-collagen-vi-in-the-alveolar-parenchyma
#8
C K Andersson, M Weitoft, K Rydell-Törmänen, L Bjermer, G Westergren-Thorsson, J S Erjefält
BACKGROUND: Asthma has been associated with increased collagen deposition in both conducting airways and alveolar parenchyma. Mast cells (MCs) are key effector cells in asthma and have the ability to affect collagen synthesis. However, the link between clinical control and changes in bronchial and alveolar MC phenotypes and specific collagens in controlled and uncontrolled asthma remains unknown. OBJECTIVE: To investigate MC phenotypes in correlation with deposition of specific collagen subtypes in patients with controlled and uncontrolled asthma as well as to healthy controls...
March 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28865408/peptide-based-vaccines-derived-from-fc%C3%AE%C2%B5ri-beta-subunit-can-reduce-allergic-response-in-mice-model
#9
Ahad Zare, Ali Akbar Pourfathollah, Zahra Pourpak, Janos Szebeni, Seyed Mohammad Reza Ghaffari, Gholam Ali Kardar
Current therapeutic approaches in allergic diseases especially asthma generally focus on using immunological strategies. According to the importance of FceRI in controlling allergic response we used two extracellular regions of Fc epsilon receptor I (FceRI) beta subunit peptides to design two peptide-based vaccines. Probably these peptides vaccines by triggering the immune response to FceRI can reduce the allergic symptoms through blocking the IgE specific receptor. Two extracellular parts of FceRI beta subunit were made by peptide synthesizer and conjugated with keyhole limpet Hemocyanin...
August 2017: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/28671980/fceri-density-and-spontaneous-secretion-from-human-basophils
#10
Donald MacGlashan
Mast cells and basophils depend on aggregation of the high-affinity IgE receptor, FceRI, to initiate secretion. A variety of studies have shown that FceRI densities vary 100 fold among subjects' basophils and it has been speculated that high densities might be responsible for unusual behaviors of the cells, notably sensitivity to certain monomeric IgE antibodies or spontaneous release. These studies experimentally examined the density dependence of spontaneous release and signaling element expression in subjects' basophils with FceRI densities ranging from approximately 6000 to 600,000 per cell...
2017: PloS One
https://www.readbyqxmd.com/read/28618453/data-driven-programmatic-approach-to-analysis-of-basophil-activation-tests
#11
Sarita U Patil, Agustin Calatroni, Michael Schneider, Johanna Steinbrecher, Neal Smith, Cecilia Washburn, Alex Ma, Wayne G Shreffler
BACKGROUND: Conventional data analysis of flow cytometry-based basophil activation testing requires repetitive, labor-intensive analysis that hampers efforts to standardize testing for clinical applications. Using an open-source platform, we developed and implemented a programmatic approach to the analysis of the basophil activation test (BAT) by flow cytometry. METHODS: Using the BÜHLMANN FlowCAST® assay, peripheral blood from peanut allergic patients undergoing oral immunotherapy was incubated with peanut allergens (Arah1, Arah2, Arah6, whole peanut extract) and stained with fluorescent antibodies to CCR3 and CD63 for the development of a data-driven programmatic analysis using Bioconductor and R...
June 15, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/27786273/the-nedd4-2-ndfip1-axis-is-a-negative-regulator-of-ige-mediated-mast-cell-activation
#12
Kwok Ho Yip, Natasha Kolesnikoff, Nicholas Hauschild, Lisa Biggs, Angel F Lopez, Stephen J Galli, Sharad Kumar, Michele A Grimbaldeston
Cross-linkage of the high-affinity immunoglobulin E (IgE) receptor (FcɛRI) on mast cells by antigen ligation has a critical role in the pathology of IgE-dependent allergic disorders, such as anaphylaxis and asthma. Restraint of intracellular signal transduction pathways that promote release of mast cell-derived pro-inflammatory mediators is necessary to dampen activation and restore homoeostasis. Here we show that the ligase Nedd4-2 and the adaptor Ndfip1 (Nedd4 family interacting protein 1) limit the intensity and duration of IgE-FcɛRI-induced positive signal transduction by ubiquitinating phosphorylated Syk, a tyrosine kinase that is indispensable for downstream FcɛRI signalosome activity...
October 27, 2016: Nature Communications
https://www.readbyqxmd.com/read/27759482/dermatologic-uses-of-omalizumabtitle
#13
REVIEW
Justin C Chia, P Régine Mydlarski
PURPOSE: Omalizumab is a recombinant humanized monoclonal antibody that inhibits the binding of immunoglobulin E (IgE) to the high-affinity IgE receptor (FceRI) on the surface of mast cells and basophils. Omalizumab has been approved for use in asthma, and new reports show promise in a variety of dermatologic diseases. Herein, we review the literature on omalizumab in dermatology and discuss the safety, efficacy and mechanisms of action for this emerging therapy. MATERIALS AND METHODS: PubMED, MEDLINE and Embase databases were searched for the period 1 January 1990 to 1 September 2016...
June 2017: Journal of Dermatological Treatment
https://www.readbyqxmd.com/read/27746735/fceri-and-histamine-metabolism-gene-variability-in-selective-responders-to-nsaids
#14
Gemma Amo, José A Cornejo-García, Jesus M García-Menaya, Concepcion Cordobes, M J Torres, Gara Esguevillas, Cristobalina Mayorga, Carmen Martinez, Natalia Blanca-Lopez, Gabriela Canto, Alfonso Ramos, Miguel Blanca, José A G Agúndez, Elena García-Martín
The high-affinity IgE receptor (Fcε RI) is a heterotetramer of three subunits: Fcε RIα, Fcε RIβ, and Fcε RIγ (αβγ2) encoded by three genes designated as FCER1A, FCER1B (MS4A2), and FCER1G, respectively. Recent evidence points to FCERI gene variability as a relevant factor in the risk of developing allergic diseases. Because Fcε RI plays a key role in the events downstream of the triggering factors in immunological response, we hypothesized that FCERI gene variants might be related with the risk of, or with the clinical response to, selective (IgE mediated) non-steroidal anti-inflammatory (NSAID) hypersensitivity...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27682583/functional-nanoscale-coupling-of-lyn-kinase-with-ige-fc%C3%AE%C2%B5ri-is-restricted-by-the-actin-cytoskeleton-in-early-antigen-stimulated-signaling
#15
Sarah A Shelby, Sarah L Veatch, David A Holowka, Barbara A Baird
The allergic response is initiated on the plasma membrane of mast cells by phosphorylation of the receptor for immunoglobulin E (IgE), FcεRI, by Lyn kinase after IgE-FcεRI complexes are cross-linked by multivalent antigen. Signal transduction requires reorganization of receptors and membrane signaling proteins, but this spatial regulation is not well defined. We used fluorescence localization microscopy (FLM) and pair-correlation analysis to measure the codistribution of IgE-FcεRI and Lyn on the plasma membrane of fixed cells with 20- to 25-nm resolution...
November 7, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27682001/association-between-perfluorooctanoic-acid-exposure-and-degranulation-of-mast-cells-in-allergic-inflammation
#16
Jun-Kyoung Lee, Soyoung Lee, Moon-Chang Baek, Byung-Heon Lee, Hyun-Shik Lee, Taeg Kyu Kwon, Pil-Hoon Park, Tae-Yong Shin, Dongwoo Khang, Sang-Hyun Kim
Perfluorooctanoic acid (PFOA) has wide applications, including as a raw material for converted paper and packaging products. With the widespread use of PFOA, concerns regarding its potential environmental and health impacts have increased. In spite of the known hepatotoxicity and genotoxicity of PFOA, correlation with PFOA and allergic inflammation is not well known. In this study, the effect of PFOA on the degranulation of mast cells and mast cell-mediated allergic inflammation in the presence of FcεRI cross-linking was evaluated...
May 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/27554051/contribution-of-fc%C3%A9-ri-associated-vesicles-to-mast-cell-macrophage-communication-following-francisella-tularensis-infection
#17
Annette R Rodriguez, Jieh-Juen Yu, Christopher Navara, James P Chambers, M Neal Guentzel, Bernard P Arulanandam
Understanding innate immune intercellular communication following microbial infection remains a key biological issue. Using live cell imaging, we demonstrate that mast cells actively extend cellular projections to sample the macrophage periphery during Francisella tularensis LVS infection. Mast cell MHCII(hi) expression was elevated from less than 1% to 13% during LVS infection. Direct contact during co-culture with macrophages further increased mast cell MHCII(hi) expression to approximately 87%. Confocal analyses of the cellular perimeter revealed mast cell caspase-1 was localized in close proximity with FcɛRI in uninfected mast cells, and repositioned to clustered regions upon LVS infection...
October 2016: Innate Immunity
https://www.readbyqxmd.com/read/27548803/isolation-and-characterization-of-hepatic-mast-cells-from-cholestatic-rats
#18
Laura Hargrove, Allyson Graf-Eaton, Lindsey Kennedy, Jennifer Demieville, Jennifer Owens, Kyle Hodges, Brittany Ladd, Heather Francis
Mast cells (MCs) are immune cells that release histamine and other mediators. MC number increases after bile duct ligation (BDL) and blocking mast cell-derived histamine decreases biliary proliferation. We aimed to isolate and characterize MCs from cholestatic livers. Rats were subjected to BDL starting at 6 h and up to 14 days. MC infiltration was evaluated by toluidine blue. BDL rats were perfused using standard collagenase perfusion. Following enzymatic digestion, tissue was passed through a fine gauge needle...
November 2016: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/27459687/immunophenotypic-and-ultrastructural-analysis-of-mast-cells-in-hermansky-pudlak-syndrome-type-1-a-possible-connection-to-pulmonary-fibrosis
#19
Arnold S Kirshenbaum, Glenn Cruse, Avanti Desai, Geethani Bandara, Maarten Leerkes, Chyi-Chia R Lee, Elizabeth R Fischer, Kevin J O'Brien, Bernadette R Gochuico, Kelly Stone, William A Gahl, Dean D Metcalfe
Hermansky-Pudlak Syndrome type-1 (HPS-1) is an autosomal recessive disorder caused by mutations in HPS1 which result in reduced expression of the HPS-1 protein, defective lysosome-related organelle (LRO) transport and absence of platelet delta granules. Patients with HPS-1 exhibit oculocutaneous albinism, colitis, bleeding and pulmonary fibrosis postulated to result from a dysregulated immune response. The effect of the HPS1 mutation on human mast cells (HuMCs) is unknown. Since HuMC granules classify as LROs along with platelet granules and melanosomes, we set out to determine if HPS-1 cutaneous and CD34+ culture-derived HuMCs have distinct granular and cellular characteristics...
2016: PloS One
https://www.readbyqxmd.com/read/27380527/inhibition-by-tranilast-of-the-synergistic-induction-of-degranulation-and-il-13-expression-by-il-33-and-fc%C3%A9-ri-cross-linking-in-mast-cells
#20
Kazuhiro Tsuji, Ken Fukuda, Atsuki Fukushima
PURPOSE: To assess the effects of tranilast on degranulation and IL-13 production in mast cells activated by IL-33 and cross-linking of FcɛRI. METHODS: Bone marrow-derived mast cells (BMMCs) were sensitized with anti-DNP IgE. Sensitized cells were pretreated with tranilast and further stimulated with DNP-BSA, IL-33, or a combination of DNP-BSA and IL-33. Degranulation and the level of IL-13 release and mRNA expression in BMMCs were measured. RESULTS: Simultaneous stimulation with DNP-BSA and IL-33 resulted in marked increase in β-hexosaminidase release...
December 2017: Ocular Immunology and Inflammation
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