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https://www.readbyqxmd.com/read/27933454/improved-production-of-adipate-with-escherichia-coli-by-reversal-of-%C3%AE-oxidation
#1
Nicolai Kallscheuer, Jochem Gätgens, Marvin Lübcke, Jörg Pietruszka, Michael Bott, Tino Polen
The linear C6 dicarboxylic acid adipic acid is an important bulk chemical in the petrochemical industry as precursor of the polymer nylon-6,6-polyamide. In recent years, efforts were made towards the biotechnological production of adipate from renewable carbon sources using microbial cells. One strategy is to produce adipate via a reversed β-oxidation pathway. Hitherto, the adipate titers were very low due to limiting enzyme activities for this pathway. In most cases, the CoA intermediates are non-natural substrates for the tested enzymes and were therefore barely converted...
December 8, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27933433/full-length-infectious-clone-of-an-iranian-isolate-of-chicken-anemia-virus
#2
Amir Kaffashi, Fatemeh Eshratabadi, Abdelhamed Shoushtari
An Iranian field strain of chicken anemia virus (CAV), designated IR CAV, was isolated in the Marek's disease virus-transformed lymphoblastoid cell line MDCC-MSB1 (MSB1) culture for the first time. The full-length CAV DNA of this strain was cloned in the bacterial plasmid pTZ57R/T to create the molecular clone pTZ-CAV. The nucleotide and deduced amino acid sequences of viral proteins of IR CAV were compared with those of representative CAV sequences including reference and commercial vaccine strains. IR CAV was not related to vaccine strains and also found to have glutamine at positions 139 and 144 confirming previous studies in which such mutations were associated with a slow rate of virus spread in cell culture...
December 8, 2016: Virus Genes
https://www.readbyqxmd.com/read/27933141/emergence-of-serotype-k1-klebsiella-pneumoniae-st23-strains-co-producing-the-plasmid-mediated-ampc-beta-lactamase-dha-1-and-an-extended-spectrum-beta-lactamase-in-korea
#3
Hae Suk Cheong, Doo Ryeon Chung, Chaeyoeng Lee, So Hyun Kim, Cheol-In Kang, Kyong Ran Peck, Jae-Hoon Song
BACKGROUND: Serotype K1 Klebsiella pneumoniae has emerged as an important community pathogen causing various infections, including liver abscesses. Although serotype K1 K. pneumoniae community isolates have been reported as susceptible to most classes of antimicrobial agents, a few cases of infection caused by extended-spectrum beta-lactamase (ESBL)-producing serotype K1 K. pneumoniae have recently been reported in Asian countries. We identified three ESBL-producing strains of serotype K1 K...
2016: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/27932754/-overexpression-of-lentivirus-rfx1-and-its-inhibitory-effect-on-proliferation-of-glioblastoma-cells
#4
Kai Cheng, Haojie Sun, Mingzhi Zhang, Li Shen
To construct overexpression lentivirus vector for human regulatory factor X1 (RFX1) gene, and to explore its effect on proliferation of F98 cell line.
 Methods: Huamn RFX1 gene was amplified by polymerase reaction. Gene amplification products were inserted into lentivirus vector pITA, and the lentivirus vector pITA-RFX1 was constructed. The constructed vector was verified by agarose gel electrophoresis and DNA sequencing. Lentivirus vector pITA-RFX1 and virus packaging plasmids were cotransfected into 293T cells, and then transfected into F98 cells...
November 28, 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/27932659/genome-sequence-of-pseudomonas-citronellolis-sjte-3-an-estrogen-and-polycyclic-aromatic-hydrocarbon-degrading-bacterium
#5
Daning Zheng, Xiuli Wang, Pingping Wang, Wanli Peng, Nannan Ji, Rubing Liang
Pseudomonas citronellolis SJTE-3, isolated from the active sludge of a wastewater treatment plant in China, can utilize a series of environmental estrogens and estrogen-like toxicants. Here, we report its whole-genome sequence, containing one circular chromosome and one circular plasmid. Genes involved in estrogen biodegradation in this bacterium were predicted.
December 8, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27932039/anaerobic-digestion-of-nitrogen-rich-poultry-manure-impact-of-thermophilic-biogas-process-on-metal-release-and-microbial-resistances
#6
Reshma Anjum, Elisabeth Grohmann, Niclas Krakat
Poultry manure is a nitrogen rich fertilizer, which is usually recycled and spread on agricultural fields. Due to its high nutrient content, chicken manure is considered to be one of the most valuable animal wastes as organic fertilizer. However, when chicken litter is applied in its native form, concerns are raised as such fertilizers also include high amounts of antibiotic resistant pathogenic Bacteria and heavy metals. We studied the impact of an anaerobic thermophilic digestion process on poultry manure...
December 5, 2016: Chemosphere
https://www.readbyqxmd.com/read/27931785/structure-biocompatibility-and-transfection-activity-relationships-of-cationic-polyaspartamides-with-dialkylamino-alkyl-and-alkyl-or-hydroxyalkyl-side-groups
#7
Diana Salakhieva, Vesta Shevchenko, Csaba Németh, Benjámin Gyarmati, András Szilágyi, Timur Abdullin
A series of 14 cationic derivatives of poly(aspartic acid) i.e. cationic polyaspartamides with different (dialkylamino)alkyl and alkyl or hydroxyalkyl side groups was synthesized by nucleophilic addition on polysuccinimide. The resulting polyaspartamides have moderate amphiphilic properties. Relationships between the structure and ratio of side groups and in vitro properties of polyaspartamides, including their cytotoxic and membrane-damaging activity towards human cell lines, primary skin fibroblasts and erythrocytes, were established and discussed...
December 5, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27930940/comparative-sequence-analysis-of-enteroaggregative-escherichia-coli-heat-stable-enterotoxin-1-identified-in-korean-and-japanese-escherichia-coli-strains
#8
Dong Joo Seo, SunKeum Choi, Su Been Jeon, Suntak Jeong, Hyunkyung Park, Bog-Hieu Lee, Geun-Bae Kim, Soo-Jin Yang, Yoshikazu Nishikawa, Changsun Choi
The aim of this study was to compare the sequence of the astA gene found in 8 Korean and 11 Japanese Escherichia coli isolates. Conventional PCR was used to amplify the astA gene from the chromosomal and plasmid DNA preparation samples of each isolate using commercial DNA extraction kits. Cloning of the PCR products, sequence analysis, and pulse field gel electrophoresis (PFGE) were sequentially performed. An identical copy of astA in each isolate were found for 8 Korean and 8 Japanese E. coli strains isolated from bovine, porcine, and healthy human carriers...
November 21, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27929123/rnai-mediated-mortality-of-the-whitefly-through-transgenic-expression-of-double-stranded-rna-homologous-to-acetylcholinesterase-and-ecdysone-receptor-in-tobacco-plants
#9
Hassan Jamil Malik, Amir Raza, Imran Amin, Jodi A Scheffler, Brian E Scheffler, Judith K Brown, Shahid Mansoor
The whitefly Bemisia tabaci (Genn.) is a pest and vector of plant viruses to crop and ornamental plants worldwide. Using RNA interference (RNAi) to down regulate whitefly genes by expressing their homologous double stranded RNAs in plants has great potential for management of whiteflies to reduce plant virus disease spread. Using a Tobacco rattle virus-derived plasmid for in planta transient expression of double stranded RNA (dsRNA) homologous to the acetylcholinesterase (AChE) and ecdysone receptor (EcR) genes of B...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27928970/effect-of-transfection-and-co-incubation-of-bovine-sperm-with-exogenous-dna-on-sperm-quality-and-functional-parameters-for-its-use-in-sperm-mediated-gene-transfer
#10
María Elena Arias, Esther Sánchez-Villalba, Andrea Delgado, Ricardo Felmer
Sperm-mediated gene transfer (SMGT) is based on the capacity of sperm to bind exogenous DNA and transfer it into the oocyte during fertilization. In bovines, the progress of this technology has been slow due to the poor reproducibility and efficiency of the production of transgenic embryos. The aim of the present study was to evaluate the effects of different sperm transfection systems on the quality and functional parameters of sperm. Additionally, the ability of sperm to bind and incorporate exogenous DNA was assessed...
December 8, 2016: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/27928692/palmitic-acid-triggers-cell-apoptosis-in-rgc-5-retinal-ganglion-cells-through-the-akt-foxo1-signaling-pathway
#11
Panshi Yan, Shu Tang, Haifeng Zhang, Yuanyuan Guo, Zhiwen Zeng, Qiang Wen
Hallmarks of the pathophysiology of glaucoma are oxidative stress and apoptotic death of retinal ganglion cells (RGCs). Lipotoxicity, involving a series of pathological cellular responses after exposure to elevated levels of fatty acids, leads to oxidative stress and cell death in various cell types. The phosphatidylinositol-3-kinase/protein kinase B/Forkhead box O1 (PI3K/Akt/FoxO1) pathway is crucial for cell survival and apoptosis. More importantly, FoxO1 gene has been reported to confer relatively higher risks for eye diseases including glaucoma...
December 7, 2016: Metabolic Brain Disease
https://www.readbyqxmd.com/read/27928483/emergence-of-cmy-2-and-dha-1-type-ampc-%C3%AE-lactamases-in-enterobacter-cloacae-isolated-from-several-hospitals-of-qazvin-and-tehran-iran
#12
Amir Peymani, Taghi Naserpour-Farivar, Moein Yeylagh-Beygi, Shahiin Bolori
BACKGROUND AND OBJECTIVES: The emergence of plasmid-mediated AmpC (pAmpC) β-lactamases conferring resistance to third-generation cephalosporins has become a major clinical concern worldwide. The aims of this study were to determine the prevalence of pAmpC-producing E. cloacae isolates and typing of them in Qazvin and Tehran provinces, Iran. MATERIALS AND METHODS: A total of 120 cefoxitin non-susceptible isolates of E. cloacae were obtained from educational hospitals of Qazvin and Tehran, Iran...
June 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/27928055/minimum-noise-production-of-translation-factor-eif4g-maps-to-a-mechanistically-determined-optimal-rate-control-window-for-protein-synthesis
#13
Xiang Meng, Helena Firczuk, Paola Pietroni, Richard Westbrook, Estelle Dacheux, Pedro Mendes, John E G McCarthy
Gene expression noise influences organism evolution and fitness. The mechanisms determining the relationship between stochasticity and the functional role of translation machinery components are critical to viability. eIF4G is an essential translation factor that exerts strong control over protein synthesis. We observe an asymmetric, approximately bell-shaped, relationship between the average intracellular abundance of eIF4G and rates of cell population growth and global mRNA translation, with peak rates occurring at normal physiological abundance...
December 7, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27927199/sh003-induces-apoptosis-of-du145-prostate-cancer-cells-by-inhibiting-erk-involved-pathway
#14
Yu-Jeong Choi, Youn Kyung Choi, Kang Min Lee, Sung-Gook Cho, Soo-Yeon Kang, Seong-Gyu Ko
BACKGROUND: Herbal medicines have been used in cancer treatment, with many exhibiting favorable side effect and toxicity profiles compared with conventional chemotherapeutic agents. SH003 is a novel extract from Astragalus membranaceus, Angelica gigas, and Trichosanthes Kirilowii Maximowicz combined at a 1:1:1 ratio that impairs the growth of breast cancer cells. This study investigates anti-cancer effects of SH003 in prostate cancer cells. METHODS: SH003 extract in 30% ethanol was used to treat the prostate cancer cell lines DU145, LNCaP, and PC-3...
December 7, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27926942/actin-related-protein-2-arp2-and-virus-induced-filopodia-facilitate-human-respiratory-syncytial-virus-spread
#15
Masfique Mehedi, Thomas McCarty, Scott E Martin, Cyril Le Nouën, Eugen Buehler, Yu-Chi Chen, Margery Smelkinson, Sundar Ganesan, Elizabeth R Fischer, Linda G Brock, Bo Liang, Shirin Munir, Peter L Collins, Ursula J Buchholz
Human respiratory syncytial virus (RSV) is an enveloped RNA virus that is the most important viral cause of acute pediatric lower respiratory tract illness worldwide, and lacks a vaccine or effective antiviral drug. The involvement of host factors in the RSV replicative cycle remains poorly characterized. A genome-wide siRNA screen in human lung epithelial A549 cells identified actin-related protein 2 (ARP2) as a host factor involved in RSV infection. ARP2 knockdown did not reduce RSV entry, and did not markedly reduce gene expression during the first 24 hr of infection, but decreased viral gene expression thereafter, an effect that appeared to be due to inhibition of viral spread to neighboring cells...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27924861/size-specific-transfection-to-mammalian-cells-by-micropillar-array-electroporation
#16
Yingbo Zu, Shuyan Huang, Yang Lu, Xuan Liu, Shengnian Wang
Electroporation serves as a promising non-viral gene delivery approach, while its current configuration carries several drawbacks associated with high-voltage electrical pulses and heterogeneous treatment on individual cells. Here we developed a new micropillar array electroporation (MAE) platform to advance the electroporation-based delivery of DNA and RNA probes into mammalian cells. By introducing well-patterned micropillar array texture on the electrode surface, the number of pillars each cell faces varies with its plasma membrane surface area, despite their large population and random locations...
December 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27924522/expression-of-%C3%AE-catenin-protein-in-hepatocellular-carcinoma-and-its-relationship-with-alpha-fetoprotein
#17
Ya-Jun Ren, Tao Huang, Hong-Lu Yu, Li Zhang, Qian-Jin He, Zhi-Fan Xiong, Hua Peng
This study aimed to investigate the expression of β-catenin in hepatocellular carcinoma (HCC) tissues and its relationship with α-fetoprotein (AFP) in HCC. Immunohistochemistry was used to determine the expression of β-catenin in normal liver tissues (n=10), liver cirrhosis tissues (n=20), and primary HCC tissues (n=60). The relationship between β-catenin expression and clinical parameters of HCC was investigated. Real-time PCR and Western blotting were used to detect the mRNA and protein expression levels of β-catenin in the liver cancer cell line SMMC-7721 transfected with a plasmid encoding AFP, and also the mRNA and protein expression levels of β-catenin were measured in the liver cancer cell line Huh7 before and after the transfection with AFP shRNA plasmids...
December 2016: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/27924153/genome-sequence-of-a-commensal-bacterium-enterococcus-faecalis-cba7120-isolated-from-a-korean-fecal-sample
#18
Joon Yong Kim, Hye Seon Song, Yeon Bee Kim, Joseph Kwon, Jong-Soon Choi, Yong-Joon Cho, Byung-Yong Kim, Jin-Kyu Rhee, Jinjong Myoung, Young-Do Nam, Seong Woon Roh
BACKGROUND: Enterococcus faecalis, the type strain of the genus Enterococcus, is not only a commensal bacterium in the gastrointestinal tract in vertebrates and invertebrates, but also causes serious disease as an opportunistic pathogen. To date, genome sequences have been published for over four hundred E. faecalis strains; however, pathogenicity of these microbes remains complicated. To increase our knowledge of E. faecalis virulence factors, we isolated strain CBA7120 from the feces of an 81-year-old female from the Republic of Korea and performed a comparative genomic analysis...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27923956/adaptation-to-potassium-limitation-is-essential-for-acinetobacter-baumannii-pneumonia-pathogenesis
#19
Reham Samir, Salma H Hussein, Noha M Elhosseiny, Marwa S Khattab, Alaa E Shawky, Ahmed S Attia
BACKGROUND:  Acinetobacter baumannii is challenging the healthcare community as the cause of a wide range of untreatable infections. New targets need to be explored for the development of therapeutics. METHODS:  The potassium-dependent protein (Kdp) system was investigated via bioinformatics and genetic tools. An isogenic mutant was constructed in kdpE and complemented in trans Gene expression and the ability to grow under potassium-limited conditions were investigated...
December 15, 2016: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27923922/mechanisms-of-evolution-in-high-consequence-drug-resistance-plasmids
#20
Susu He, Michael Chandler, Alessandro M Varani, Alison B Hickman, John P Dekker, Fred Dyda
: The dissemination of resistance among bacteria has been facilitated by the fact that resistance genes are usually located on a diverse and evolving set of transmissible plasmids. However, the mechanisms generating diversity and enabling adaptation within highly successful resistance plasmids have remained obscure, despite their profound clinical significance. To understand these mechanisms, we have performed a detailed analysis of the mobilome (the entire mobile genetic element content) of a set of previously sequenced carbapenemase-producing Enterobacteriaceae (CPE) from the National Institutes of Health Clinical Center...
December 6, 2016: MBio
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