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https://www.readbyqxmd.com/read/28426026/generation-of-multipotent-induced-cardiac-progenitor-cells-from-mouse-fibroblasts-and-potency-testing-in-ex-vivo-mouse-embryos
#1
Pratik A Lalit, Adriana M Rodriguez, Karen M Downs, Timothy J Kamp
Here we describe a protocol to generate expandable and multipotent induced cardiac progenitor cells (iCPCs) from mouse adult fibroblasts using forced expression of Mesp1, Tbx5, Gata4, Nkx2.5 and Baf60c (MTGNB) along with activation of Wnt and JAK/STAT signaling. This method does not use iPS cell factors and thus differs from cell activation and signaling-directed (CASD) reprogramming to cardiac progenitors. Our method is specific to direct CPC reprogramming, whereas CASD reprogramming can generate various cell types depending on culture conditions and raises the possibility of transitioning through a pluripotent cell state...
May 2017: Nature Protocols
https://www.readbyqxmd.com/read/28425064/a-pathway-to-personalizing-therapy-for-metastases-using-liver-on-a-chip-platforms
#2
A S Khazali, A M Clark, A Wells
Metastasis accounts for most cancer-related deaths. The majority of solid cancers, including those of the breast, colorectum, prostate and skin, metastasize at significant levels to the liver due to its hemodynamic as well as tumor permissive microenvironmental properties. As this occurs prior to detection and treatment of the primary tumor, we need to target liver metastases to improve patients' outcomes. Animal models, while proven to be useful in mechanistic studies, do not represent the heterogeneity of human population especially in drug metabolism lack proper human cell-cell interactions, and this gap between animals and humans results in costly and inefficient drug discovery...
April 19, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28421002/application-of-stem-cells-in-oral-disease-therapy-progresses-and-perspectives
#3
REVIEW
Bo Yang, Yi Qiu, Niu Zhou, Hong Ouyang, Junjun Ding, Bin Cheng, Jianbo Sun
Stem cells are undifferentiated and pluripotent cells that can differentiate into specialized cells with a more specific function. Stem cell therapies become preferred methods for the treatment of multiple diseases. Oral and maxillofacial defect is one kind of the diseases that could be most possibly cured by stem cell therapies. Here we discussed oral diseases, oral adult stem cells, iPS cells, and the progresses/challenges/perspectives of application of stem cells for oral disease treatment.
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28420858/transcriptional-regulation-of-pancreas-development-and-%C3%AE-cell-function-review
#4
Yoshio Fujitani
A small number of cells in the adult pancreas are endocrine cells. They are arranged in clusters called islets of Langerhans. The islets make insulin, glucagon, and other endocrine hormones, and release them into the blood circulation. These hormones help control the level of blood glucose. Therefore, a dysfunction of endocrine cells in the pancreas results in impaired glucose homeostasis, or diabetes mellitus. The pancreas is an organ that originates from the evaginations of pancreatic progenitor cells in the epithelium of the forgut endoderm...
April 14, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28414743/the-et-1-mediated-carbonylation-and-degradation-of-anxa1-induce-inflammatory-phenotype-and-proliferation-of-pulmonary-artery-smooth-muscle-cells-in-hps
#5
Jing He, Bin Yi, Yang Chen, Qing Huang, Huan Wang, Kaizhi Lu, Weiling Fu
Hepatopulmonary syndrome (HPS) is a serious complication of advanced liver disease, which markedly increases mortality. Pulmonary vascular remodelling (PVR) induced by circulating mediators plays an important role in the pathogenesis of HPS, while the underlying mechanism remains undefined. In the present study, we reported that endothelin-1 (ET-1) is up-regulated and annexin A1(ANXA1) is down-regulated in HPS rat, and ET-1 decreases the ANXA1 expression in a dose-dependent manner in rat pulmonary arterial smooth muscle cells (PASMCs)...
2017: PloS One
https://www.readbyqxmd.com/read/28413817/dataset-in-support-of-the-generation-of-niemann-pick-disease-type-c1-patient-specific-ips-cell-lines-carrying-the-novel-npc1-mutation-c-1180t-c-or-the-prevalent-c-3182t-c-mutation-analysis-of-pluripotency-and-neuronal-differentiation
#6
Franziska Peter, Michaela Trilck, Michael Rabenstein, Arndt Rolfs, Moritz J Frech
Data presented in this article demonstrate the generation and characterization of two novel Niemann-Pick disease Type C1 (NPC1) patient-specific induced pluripotent stem cell (iPSC) lines, related to the research article Trilck et al. (Diversity of Glycosphingolipid GM2 and Cholesterol Accumulation in NPC1 Patient-Specific iPSC-Derived Neurons; Brain Res.; 2017; 1657:52-61. doi: 10.1016/j.brainres.2016.11.031). For reprogramming fibroblasts, carrying the novel homozygous mutation c.1180T>C and the prevalent homozygous mutation c...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28413770/biophysical-regulation-of-cell-reprogramming
#7
Sze Yue Wong, Jennifer Soto, Song Li
Induced pluripotent stem (iPS) cell reprogramming and direct reprogramming are promising approaches for disease modeling and personalized medicine. However, these processes are yet to be optimized. Biomaterials are increasingly integrated into cell reprogramming strategies in order to engineer the microenvironment, improve reprogramming efficiency and achieve effective in situ cell reprogramming. Although there are some studies on the role of biomaterials in iPS cell reprogramming, their effect on direct cell conversion has not been fully explored...
February 2017: Current Opinion in Chemical Engineering
https://www.readbyqxmd.com/read/28407125/cell-fate-modification-toward-the-hepatic-lineage-by-extrinsic-factors
#8
Masaki Kawamata, Atsushi Suzuki
The lineage of a somatic cell can be altered by targeting its signaling networks with small molecules and/or genetically altering the expression of key transcription factors. Depending on the combination of factors, fibroblasts can be fully reprogrammed into induced pluripotent stem (iPS) cells or directly converted into specific cell lineages, bypassing the pluripotent state. The generation of defined target cells will enormously benefit patients who require cell transplantation therapy. In the decade since iPS cells were first generated, many cell types have been induced from fibroblasts by direct conversion, including hepatocytes...
April 12, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28399172/in-silico-design-of-the-first-dna-independent-mechanism-based-inhibitor-of-mammalian-dna-methyltransferase-dnmt1
#9
Vedran Miletić, Ivica Odorčić, Patrik Nikolić, Željko M Svedružić
BACKGROUND: We use our earlier experimental studies of the catalytic mechanism of DNA methyltransferases to prepare in silico a family of novel mechanism-based inhibitors of human Dnmt1. Highly specific inhibitors of DNA methylation can be used for analysis of human epigenome and for the creation of iPS cells. RESULTS: We describe a set of adenosyl-1-methyl-pyrimidin-2-one derivatives as novel mechanism-based inhibitors of mammalian DNA methyltransferase Dnmt1. The inhibitors have been designed to bind simultaneously in the active site and the cofactor site and thus act as transition-state analogues...
2017: PloS One
https://www.readbyqxmd.com/read/28395810/generation-of-non-integrated-induced-pluripotent-stem-cells-from-a-23-year-old-male-with-multiple-endocrine-neoplasia-type-1-syndrome
#10
Dongsheng Guo, Feima Wu, Haikun Liu, Ge Gao, Shanglong Kou, Fan Yang, Nasir Abbas, Tiancheng Zhou, Xiujuan Cai, Hui Zhang, Dajiang Qin, Jialiang Li, Kecheng Xu, Yin-Xiong Li
Urine resource cells were collected from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids. Two stable iPSC lines with free of episomal plasmid were established. The patient has a heterozygous G>T mutation on the exon 9 of Men1 gene that was confirmed by sequencing analysis on all resulted cell lines. Karyotyping indicated the chromosomes with normal appearances and numbers. Their pluripotency was demonstrated by gene expression and their abilities for differentiating into three germ layers...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395808/generation-of-non-integrated-induced-pluripotent-stem-cells-from-a-59-year-old-female-with-multiple-endocrine-neoplasia-type-1-syndrome
#11
Dongsheng Guo, Feima Wu, Haikun Liu, Ge Gao, Shanglong Kou, Fan Yang, Nasir Abbas, Tiancheng Zhou, Xiujuan Cai, Hui Zhang, Dajiang Qin, Jialiang Li, Kecheng Xu, Yin-Xiong Li
Urine resource cells were collected from a 59-year-old female patient with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids carrying Oct4, Sox2, Klf4 and miR-302-367. The patient sustained a heterozygous G>T transition mutation on the exon 9 of Men1 gene that was confirmed by sequencing analysis on the obtained iPSC lines. Karyotyping indicated the chromosomes with normal appearances and numbers. Their pluripotency was demonstrated by gene expression, as well as their abilities for differentiating into three germ layers...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395796/derivation-of-human-ips-cell-lines-from-monozygotic-twins-in-defined-and-xeno-free-conditions
#12
Elias Uhlin, Harriet Rönnholm, Kelly Day, Malin Kele, Kristiina Tammimies, Sven Bölte, Anna Falk
Human induced pluripotent stem (hiPS) cell lines CTRL-9-II and CTRL-10-I were derived from healthy monozygotic twin donors using non-integrating RNA based Sendai virus reprogramming and cultured in a xeno-free chemically defined condition. The established hiPS cell lines, CTRL-9-II and CTRL-10-I, are karyotypically normal, free from reprogramming vectors, display endogenously expression of pluripotency factors at levels similar to embryonic stem cells. The generated iPS cell lines demonstrate pluripotency by passing bioinformatics assay PluriTest and by embryonic body assay...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395795/generation-of-a-disease-specific-ips-cell-line-derived-from-a-patient-with-charcot-marie-tooth-type-2k-lacking-functional-gdap1-gene
#13
Salvador Martí, Marian León, Carmen Orellana, Javier Prieto, Xavier Ponsoda, Carlos López-García, Juan Jesús Vílchez, Teresa Sevilla, Josema Torres
Human CMT2-FiPS4F1 cell line was generated from fibroblasts of a patient with Charcot-Marie-Tooth disease harbouring the following mutations in the GDAP1 gene in heterozygosis: p.Q163X/p.T288NfsX3. This patient did not present mutations in the PM22, MPZ or GJB genes. Human reprogramming factors OCT3/4, KLF4, SOX2 and C-MYC were delivered using a non-integrative methodology that involves the use of Sendai virus.
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395739/generation-of-an-ipsc-line-from-a-patient-with-gtp-cyclohydrolase-1-gch1-deficiency-hdmc0061i-gch1
#14
Sabine Jung-Klawitter, Juliane Ebersold, Gudrun Göhring, Nenad Blau, Thomas Opladen
Fibroblasts from a female patient carrying a heterozygous variation in GTP cyclohydrolase 1 (GCH1; OMIM: 600225; HGNC: 4193; c.235_240del/p.(L79_S80del)), the rate-limiting enzyme of tetrahydrobiopterin (BH4) synthesis, were reprogrammed to iPSCs using the Cytotune®-iPS 2.0 Sendai Reprogramming Kit (Invitrogen) delivering the four reprogramming factors Oct3/4, Sox2, c-Myc and Klf4. Pluripotency of HDMC0061i-GCH1 was verified using immunohistochemistry and RT-PCR analysis. Cells differentiated spontaneously into the 3 germ layers in vitro and presented a normal karyotype...
April 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28393187/generation-and-characterization-of-induced-pluripotent-stem-cells-from-guinea-pig-fetal-fibroblasts
#15
Yuehong Wu, Ouyang Li, Chengwen He, Yong Li, Min Li, Xiaoming Liu Liu, Yujiong Wang, Yulong He
Induced pluripotent stem cells (iPS) represent an important tool to develop disease‑modeling assays, drug testing assays and cell‑based replacement therapies. The application of iPS in these fields requires the development of suitable animal models. Of the suitable species, guinea pigs are particularly important and offer significant advantages. Successful iPS generation has been accomplished in a number of species; however, it has not been reported in the guinea pig. The present study successfully generated iPS from guinea pigs (giPS) using single polycistronic virus transduction with mouse octamer‑binding transcription factor 4 (Oct4), sex determining region Y‑box 2 (Sox2), Kruppel‑like factor 4 and c‑Myc...
April 4, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28388235/applications-of-crispr-genome-editing-technology-in-drug-target-identification-and-validation
#16
Quinn Lu, George P Livi, Sundip Modha, Kosuke Yusa, Ricardo Macarrón, David J Dow
Structured Abstract Introduction: The analysis of pharmaceutical industry data indicates that the major reason for drug candidates failing in late stage clinical development is lack of efficacy, with a high proportion of these due to erroneous hypotheses about target to disease linkage. More than ever, there is a requirement to better understand potential new drug targets and their role in disease biology in order to reduce attrition in drug development. Genome editing technology enables precise modification of individual protein coding genes, as well as noncoding regulatory sequences, enabling the elucidation of functional effects in human disease relevant cellular systems...
April 7, 2017: Expert Opinion on Drug Discovery
https://www.readbyqxmd.com/read/28387938/chick-derived-induced-pluripotent-stem-cells-by-the-poly-cistronic-transposon-with-enhanced-transcriptional-activity
#17
Masafumi Katayama, Takashi Hirayama, Tetsuya Tani, Katsuhiko Nishimori, Manabu Onuma, Tomokazu Fukuda
Induced pluripotent stem (iPS) cell technology lead terminally differentiated cells into the pluripotent stem cells through the expression of defined reprogramming factors. Although iPS cells have been established in a number of mammalian species, including mouse, human, and monkey, studies on iPS cells in avian species are still very limited. To establish chick iPS cells, six factors were used within the poly-cistronic reprogramming vector (PB-R6F), containing M3O (MyoD derived transactivation domain fused with Oct3/4), Sox2, Klf4, c-Myc, Lin28, and Nanog...
April 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28386481/ips-cell-technologies-and-their-prospect-for-bone-regeneration-and-disease-modeling-a-mini-review
#18
REVIEW
Maria Csobonyeiova, Stefan Polak, Radoslav Zamborsky, Lubos Danisovic
Bone disorders are a group of varied acute and chronic traumatic, degenerative, malignant or congenital conditions affecting the musculoskeletal system. They are prevalent in society and, with an ageing population, the incidence and impact on the population's health is growing. Severe persisting pain and limited mobility are the major symptoms of the disorder that impair the quality of life in affected patients. Current therapies only partially treat the disorders, offering management of symptoms, or temporary replacement with inert materials...
July 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28386058/cartilage-tissue-engineering-by-the-3d-bioprinting-of-ips-cells-in-a-nanocellulose-alginate-bioink
#19
Duong Nguyen, Daniel A Hägg, Alma Forsman, Josefine Ekholm, Puwapong Nimkingratana, Camilla Brantsing, Theodoros Kalogeropoulos, Samantha Zaunz, Sebastian Concaro, Mats Brittberg, Anders Lindahl, Paul Gatenholm, Annika Enejder, Stina Simonsson
Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients. As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes. Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA). Low proliferation and phenotypic changes away from pluripotency were seen in the case of NFC/HA...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28384031/polarized-secretion-of-matrix-metalloproteinases-and-their-inhibitors-by-retinal-pigment-epithelium-derived-from-induced-pluripotent-stem-cells-during-wound-healing
#20
Whitney A Greene, Teresa A Burke, Ramesh R Kaini, Elaine D Por, Heuy-Ching Wang
PURPOSE: To characterize the secretion of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) by induced pluripotent stem cell-derived retinal pigment epithelium (iPS-RPE) during wound healing. We hypothesize that iPS-RPE secretes mediators of tissue remodeling such as MMPs and TIMPs to promote migration and proliferation of cells during wound healing. METHODS: iPS-RPE was grown on transwells until fully confluent and pigmented...
April 2017: Journal of Ocular Pharmacology and Therapeutics
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