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https://www.readbyqxmd.com/read/30119053/using-the-hybrid-operating-room-in-thoracic-surgery-a-paradigm-shift
#1
Carsten Schroeder, Jane M Chung, Andrew B Mitchell, Thomas A Dillard, Alessandro G Radaelli, Stephanie Schampaert
We describe the integration of the hybrid operation room cone-beam computed tomography (CT) scan technology into the practice of general thoracic surgery. The combination of the following three techniques: (1) cone-beam CT scan augmented navigational bronchoscopy, (2) cone-beam CT-guided percutaneous biopsy and/or fiducial placement, and (3) fiducial or image-guided video-assisted thoracic surgery resection, into a single-stage, single-provider procedure allows for diagnosis and treatment in one setting. Rapid on-site evaluation of cytological or pathology specimens is key to this "all-in-one" approach...
August 17, 2018: Innovations: Technology and Techniques in Cardiothoracic and Vascular Surgery
https://www.readbyqxmd.com/read/30118703/detection-of-three-common-mutations-causing-%C3%AE-thalassemia-by-using-a-closed-tube-multiplex-pcr
#2
Xingyuan Jia, Limei Yao, Yan Zhou, Han Han, Ning Tang, Lirong Wang, Ying Li
Small oligonucleotides mutations are the large majority causes of β-thalassemia. Dual priming oligonucleotide PCR has been used to detect point mutations and thus could be applied to diagnose β-thalassemia. The goal of this study was to establish a simple, quick and cost-effective screening assay by using modified dual priming oligonucleotide PCR for three most common mutations of β-thalassemia [CD71-72 (+A), CD 41-42 (-CTTT), Pnt.-28 (A → G)] in Southeast Asia and southern China. Man-made 5 tandem mismatched bases instead of poly (I) were used as the linker in the specific PCR primers...
August 14, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/30118525/tlr4-mediated-activation-of-the-erk-pathway-following-uva-irradiation-contributes-to-increased-cytokine-and-mmp-expression-in-senescent-human-dermal-fibroblasts
#3
Seong-Wook Seo, Seul-Ki Park, Soo-Jin Oh, Ok Sarah Shin
Exposure to ultraviolet (UV) radiation is a major contributing factor to premature aging (photoaging) and skin cancer. In vitro models of cellular senescence have proven to be very useful for the study of slow and progressive accumulation of damage resulting in the growth arrest of aging skin cells. In this study, we compared UVA-induced cellular responses in non-senescent (NS) vs. senescent (S) human dermal fibroblasts (HDFs). HDFs were irradiated with a single dose of UVA (7.5 J/cm2) and QuantSeq 3' mRNA sequencing was performed to assess differential gene expression...
2018: PloS One
https://www.readbyqxmd.com/read/30118517/population-genetic-analysis-of-aquaculture-salmonid-populations-in-china-using-a-57k-rainbow-trout-snp-array
#4
Han-Yuan Zhang, Zi-Xia Zhao, Jian Xu, Peng Xu, Qing-Li Bai, Shi-Yong Yang, Li-Kun Jiang, Bao-Hua Chen
Various salmonid species are cultivated in cold water aquaculture. However, due to limited genomic data resources, specific high-throughput genotyping tools are not available to many of the salmonid species. In this study, a 57K single nucleotide polymorphism (SNP) array for rainbow trout (Oncorhynchus mykiss) was utilized to detect polymorphisms in seven salmonid species, including Hucho taimen, Oncorhynchus masou, Salvelinus fontinalis, Brachymystax lenok, Salvelinus leucomaenis, O. kisutch, and O. mykiss...
2018: PloS One
https://www.readbyqxmd.com/read/30118440/design-and-performance-of-the-micro-dose-calibrator
#5
Stephen S Adler, Peter Choyke
A new class of dose calibrator called a micro-dose calibrator, designed to measure radioactivity rates in the range between 1 kBq up to 370 kBq, has been designed and an 8 NaI(Tl)/photo multiplier tube (PMT) module prototype built to evaluate its performance. The design of the micro-dose calibrator is based on the concept of a segmented well counter. By segmenting a single crystal well counter into multiple crystals or segments, with each segment operated and read out independently, one can extend the operational range of a standard single crystal well counter while maintaining its accuracy and spectrographic capabilities...
August 17, 2018: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/30118395/occurrence-of-deoxynivalenol-in-an-elderly-cohort-in-the-uk-a-biomonitoring-approach
#6
Maria Papageorgiou, Liz Wells, Courtney Williams, Kay L M White, Barbara De Santis, Yunru Liu, Francesca Debegnach, Brunella Miano, Giorgio Moretti, Stephanie Greentham, Carlo Brera, Stephen L Atkin, Laura J Hardie, Thozhukat Sathyapalan
Deoxynivalenol (DON) is a Fusarium toxin, to which humans are frequently exposed via diet. Although the elderly are speculated to be sensitive to the toxic effects of DON as a result of age-related conditions, disease and altered DON metabolism, there is lack of available data on DON biomarkers in this age group. This study characterised urinary DON concentrations and its metabolites in elderly aged ≥65years (n = 20) residing in Hull, UK. Morning urinary specimens were collected over two consecutive days together with food records to assess dietary intake over a 24h-period prior to each urinary collection...
August 17, 2018: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/30118288/single-atom-detection-from-low-contrast-to-noise-ratio-electron-microscopy-images
#7
J Fatermans, A J den Dekker, K Müller-Caspary, I Lobato, C M O'Leary, P D Nellist, S Van Aert
Single atom detection is of key importance to solving a wide range of scientific and technological problems. The strong interaction of electrons with matter makes transmission electron microscopy one of the most promising techniques. In particular, aberration correction using scanning transmission electron microscopy has made a significant step forward toward detecting single atoms. However, to overcome radiation damage, related to the use of high-energy electrons, the incoming electron dose should be kept low enough...
August 3, 2018: Physical Review Letters
https://www.readbyqxmd.com/read/30118284/development-of-ferromagnetic-fluctuations-in-heavily-overdoped-bi-pb-_-2-sr_-2-cuo_-6-%C3%AE-copper-oxides
#8
Koshi Kurashima, Tadashi Adachi, Kensuke M Suzuki, Yasushi Fukunaga, Takayuki Kawamata, Takashi Noji, Hitoshi Miyasaka, Isao Watanabe, Masanori Miyazaki, Akihiro Koda, Ryosuke Kadono, Yoji Koike
We demonstrate the presence of ferromagnetic (FM) fluctuations in the superconducting and nonsuperconducting heavily overdoped regimes of high-temperature superconducting copper oxides, using (Bi,Pb)_{2}Sr_{2}CuO_{6+δ} (Bi-2201) single crystals. Magnetization curves exhibit a tendency to be saturated in high magnetic fields at low temperatures in the heavily overdoped crystals, which is probably a precursor phenomenon of a FM transition at a lower temperature. Muon spin relaxation detects the enhancement of spin fluctuations at high temperatures below 200 K...
August 3, 2018: Physical Review Letters
https://www.readbyqxmd.com/read/30118057/one-symbol-training-receiver-for-the-spad-based-uvlc-system
#9
Chao Wang, Hongyi Yu, Yi-Jun Zhu, Tao Wang, Yawei Ji
For the long distance single photon avalanche diode (SPAD)-based underwater visible light communication (UVLC) system, the multiple-symbol union detection scheme is presented. However, an error floor curve of the bit-to-error ratio (BER) occurs and cannot vanish even though the transmitted power approaches infinity. In this paper, to solve the problems of error floor and channel estimation in the long distance SPAD-based UVLC system, we propose the one training symbol maximum likelihood (ML) detection receiver...
July 10, 2018: Applied Optics
https://www.readbyqxmd.com/read/30118008/polarization-insensitive-broadband-terahertz-metamaterial-absorber-based-on-hybrid-structures
#10
Yuying Lu, Jining Li, Shaohua Zhang, Jinhai Sun, Jian Quan Yao
A polarization-insensitive broadband terahertz (THz) metamaterial absorber is presented in this paper. The metamaterial absorber consists of several circular and rhombus metallic patches as a unit cell and a metallic ground plane separated by a polyimide dielectric layer. By increasing the number of circular and rhombus patches with different sizes, the operating bandwidth of the absorber is broadened because of the resonances of each single patch getting close enough and merging together in the frequency range...
July 20, 2018: Applied Optics
https://www.readbyqxmd.com/read/30117943/wideband-infrared-trap-detector-based-upon-doped-silicon-photocurrent-devices
#11
S I Woods, J E Proctor, T M Jung, A C Carter, J Neira, D R Defibaugh
We have designed, fabricated, and measured infrared trap detectors made from arsenic-doped silicon (Si:As) blocked impurity band (BIB) photodetectors. These trap detectors are composed of two detectors in a wedge geometry, with an entrance aperture diameter of either 1 or 3 mm. The detectors were calibrated for quantum efficiency against a pyroelectric reference detector using a Fourier transform spectral comparator system, and etalon effects and spatial uniformity of the traps were also quantified. Measurements of the traps at a temperature of 10 K show that nearly ideal external quantum efficiency (>90%) can be attained over much of the range from 4 to 24 μm, with significant responsivity from 2 to 30 μm...
June 20, 2018: Applied Optics
https://www.readbyqxmd.com/read/30117893/compact-portable-laser-system-for-mobile-cold-atom-gravimeters
#12
Xiaowei Zhang, Jiaqi Zhong, Biao Tang, Xi Chen, Lei Zhu, Panwei Huang, Jin Wang, Mingsheng Zhan
We have designed and realized a compact portable laser system for high-sensitivity mobile cold atom interferometers. The laser system is mounted on a single module with dimensions of 45  cm×45  cm×16  cm and emits lights directly on up to 13 fiber ports for a two-dimensional magneto-optical trap, atom fountain, Raman transition, and normalized detection. A double-sided optical structure and mounts without kinematic adjustment are designed to achieve high-level integration and stability. The laser system is applied to a mobile atom gravimeter and achieves a sensitivity of 28  μGal/Hz1/2 ...
August 1, 2018: Applied Optics
https://www.readbyqxmd.com/read/30117820/phase-stable-downconverting-microwave-photonic-link-with-analysis-of-balanced-heterodyne-detection-intermodulation
#13
Calvin Harrington, Tia Sharpe, Jason Dahl, Zeb W Barber
A coherent downconverting microwave photonic link using balanced heterodyne detection of single-sideband modulated optical signals is analyzed and demonstrated. The intermodulation analysis shows that the second-order photodetector nonlinearity can be the limiting factor in the third-order intermodulation spur performance. The link is designed to phase coherently downconvert 17 GHz of bandwidth in an extended K-band (23-45 GHz) to 1-18 GHz to match the capabilities of modern receivers. The application of the link is for a low-loss >20  μs analog buffer...
July 1, 2018: Applied Optics
https://www.readbyqxmd.com/read/30117735/obtaining-chemical-selectivity-from-a-single-non-selective-sensing-film-two-stage-adaptive-estimation-scheme-with-multiparameter-measurement-to-quantify-mixture-components-and-interferents
#14
Karthick Sothivelr, Florian Bender, Fabien Josse, Edwin E Yaz, Antonio J Ricco
A new approach is reported to detect and quantify the members of a group of small-aromatic-molecule target analytes: benzene, toluene, ethylbenzene and xylenes (BTEX), dissolved in water, in the presence of interferents, using only the data collected from a single polymer-coated SH-SAW (shear horizontal surface acoustic wave) device and a two-stage adaptive estimation scheme. This technique is comprised of exponentially-weighted recursive least-squares estimation (EW-RLSE) and a bank of Kalman filters (BKFs) and does not require any prior knowledge of the initial concentration range of the target analytes...
August 17, 2018: ACS Sensors
https://www.readbyqxmd.com/read/30117668/sulforaphane-absorption-and-histone-deacetylase-activity-following-single-dosing-of-broccoli-sprout-supplement-in-normal-dogs
#15
Kaitlin M Curran, Shay Bracha, Carmen P Wong, Laura M Beaver, Jan F Stevens, Emily Ho
The role of epigenetic alterations during cancer has gained increasing attention and has resulted in a paradigm shift in our understanding of mechanisms leading to cancer susceptibility. Sulforaphane (SFN) is a naturally occurring isothiocyanate derived from the precursor glucosinolate, glucoraphanin (GFN), which is found in cruciferous vegetables such as broccoli. Sulforaphane has been shown to suppress tumour growth by several mechanisms including inhibiting histone deacetylases. The objective of this study was to provide a detailed analysis of sulforaphane absorption following a single oral dose of a broccoli sprout supplement in normal dogs...
August 17, 2018: Veterinary Medicine and Science
https://www.readbyqxmd.com/read/30117617/development-of-a-diagnostic-system-for-detection-of-specific-antibodies-and-antigens-against-middle-east-respiratory-syndrome-coronavirus
#16
Kunse Lee, Hae Li Ko, Eun-Young Lee, Hyo-Jung Park, Young Seok Kim, Yeon-Sook Kim, Nam-Hyuk Cho, Man-Seong Park, Sang-Myeong Lee, Jihye Kim, Hun Kim, Baik Lin Seong, Jae-Hwan Nam
Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high rate of fatality. The binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 (DDP4) is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protecting against viral outbreaks...
August 17, 2018: Microbiology and Immunology
https://www.readbyqxmd.com/read/30117422/-detection-of-ugt1a1-6-single-nucleotide-polymorphism-in-oral-swap-samples-using-the-cascade-invader-assay-based-real-time-pcr
#17
Jie Yu Zhang, Ya Nan Chu, Bing Jie Zou, Yan Jie Zhang, Li Ying Feng
The adverse reaction to irinotecan is related to the single nucleotide polymorphism (SNP) of UGT1A1*6 genotype. The current SNP detection methods have various disadvantages, including time-consuming procedures, high- risk cross-contamination, and cumbersome operation. Hence, it is necessary to establish a new method suitable for clinical application, which is easy and simple to detect SNP with minimal risk for cross-contamination. In this study, a cascade invader assay-based real-time PCR, for UGT1A1*6 genotyping has been established by optimizing reaction conditions with DNA samples of three genotypes...
August 16, 2018: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/30117418/-comparison-of-different-single-cell-whole-genome-amplification-methods-and-malbac-applications-in-assisted-reproduction
#18
Ya Xin Yao, Yong Fu La, Ran Di, Qiu Yue Liu, Wen Ping Hu, Xiang Yu Wang, Ming Xing Chu
Single-cell whole genome amplification (WGA) is a new technology, which can amplify small amounts of DNA from single cell and obtain the high coverage whole genome DNA template for revealing cell heterogeneity. Single cell WGA methods mainly include primer extension preamplification PCR (PEP-PCR), degenerate oligonucleotide primed PCR (DOP-PCR), multiple displacement amplification (MDA), and multiple annealing and looping-based amplification cycles (MALBAC). In this review, we describe the principles and applications of different single cell genome wide amplification, and we evaluate and compare their amplification efficiency, including the coverage of genome, homogeneity, reproducibility, and detection power of single-nucleotide variants (SNV) and copy number variants (CNV)...
August 16, 2018: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/30117193/microencapsulated-adult-porcine-islets-transplanted-intraperitoneally-in-streptozotocin-diabetic-non-human-primates
#19
Susan A Safley, Norma S Kenyon, Dora M Berman, Graham F Barber, Melissa Willman, Stephanie Duncanson, Neal Iwakoshi, Robert Holdcraft, Lawrence Gazda, Peter Thompson, I Raul Badell, Athanassios Sambanis, Camillo Ricordi, Collin J Weber
BACKGROUND: Xenogeneic donors would provide an unlimited source of islets for the treatment of type 1 diabetes (T1D). The goal of this study was to assess the function of microencapsulated adult porcine islets (APIs) transplanted ip in streptozotocin (STZ)-diabetic non-human primates (NHPs) given targeted immunosuppression. METHODS: APIs were encapsulated in: (a) single barium-gelled alginate capsules or (b) double alginate capsules with an inner, islet-containing compartment and a durable, biocompatible outer alginate layer...
August 17, 2018: Xenotransplantation
https://www.readbyqxmd.com/read/30116963/extra-target-low-risk-prostate-cancer-implications-for-focal-high-intensity-focused-ultrasound-of-clinically-significant-prostate-cancer
#20
A Annoot, J Olivier, P Valtille, V Deken, X Leroy, P Puech, A Villers
PURPOSE: To analyse the impact of the presence of extra-target non-clinically significant cancer (NCSC) after high-intensity focused ultrasound (HIFU) hemiablation on oncological results. To analyse radical treatment free survival (RTFS) rates at 2-3 years follow-up. METHODS: Retrospective single-centre study of 55 patients treated by primary HIFU hemiablation from 2010 to 2016. Inclusion criteria were unilateral MRI detected CSC, stage ≤ T2b, Gleason score (GS) ≤ 7, at least 6 mm distant from prostate apex...
August 16, 2018: World Journal of Urology
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