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https://www.readbyqxmd.com/read/30517974/simultaneous-quantitation-of-metformin-and-dapagliflozin-in-human-plasma-by-lc-ms-ms-application-to-a-pharmacokinetic-study
#1
Priyanka A Shah, Pranav S Shrivastav, Jaivik V Shah, Archana George
A single LC-MS/MS assay is developed and validated for simultaneous determination of metformin and dapagliflozin in human plasma using ion-pair solid phase extraction. Chromatographic separation of the analytes and their internal standards was carried out on a reversed-phase ACE 5CN (150 × 4.6 mm, 5 μm) column using acetonitrile-15 mM ammonium acetate, pH 4.5 (70:30, v/v) as the mobile phase. To achieve higher sensitivity and selectivity for the analytes, mass spectrometric analysis was performed using polarity switching approach...
December 5, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/30517932/correlation-of-ultrasound-findings-and-fine-needle-aspiration-cytology-for-the-diagnosis-of-axillary-lymph-node-metastasis-in-patients-with-breast-carcinoma
#2
Kusum Kapila, Preetha Alath, Govind H Hebbar, Mohammed Jaragh, Sara S George, Ayesha AlJassar
OBJECTIVE: Ultrasound-guided fine-needle aspiration cytology (USG-FNAC) is used for the detection of axillary lymph node (ALN) metastasis in patients with breast carcinoma (BC). US findings have a good diagnostic accuracy with high sensitivity and specificity. The aim of this study is to correlate the detection of ALN metastases on US with FNAC in BC patients. STUDY DESIGN: In 75 BC patients, over a period of 9 months (January to September 2017), the size, cortical thickness (CT), presence or absence of hilar fat, and length/width ratio of ALN on US were reviewed and correlated with FNAC findings...
December 5, 2018: Acta Cytologica
https://www.readbyqxmd.com/read/30517782/ingaas-gaas-nanowire-avalanche-photodiodes-toward-single-photon-detection-in-free-running-mode
#3
Alan Cervantes Farrell, Xiao Meng, Dingkun Ren, Hyunseok Kim, Pradeep Senanayake, Nick Y Hsieh, Zixuan Rong, Ting-Yuan Chang, Khalifa Mohammad Azizur-Rahman, Diana L Huffaker
Single photon detection at near-infrared (NIR) wavelengths is critical for light detection and ranging (LiDAR) systems used in imaging technologies such as autonomous vehicle trackers and atmospheric remote sensing. Portable, high-performance LiDAR relies on silicon-based single-photon avalanche diodes (SPADs) due to their extremely low dark count rate (DCR) and afterpulsing probability, but their operation wavelengths are typically limited up to 905 nm. Although InGaAs-InP SPADs offer an alternative platform to extend the operation wavelengths to eye-safe ranges, their high DCR and afterpulsing severely limit their commercial applications...
December 5, 2018: Nano Letters
https://www.readbyqxmd.com/read/30517712/an-experimental-study-of-diazepam-and-alprazolam-kinetics-in-urine-and-oral-fluid-following-single-oral-doses
#4
Vidar Temte, Kari Kjeldstadli, Lina Dorthea Bruun, Morris Birdal, Liliana Bachs, Ritva Karinen, Gerrit Middelkoop, Elisabeth Øiestad, Gudrun Høiseth
Benzodiazepines are commonly seen in samples submitted for drug testing of patients, people involved in child welfare cases, work-place drug testing, as well as in drug-facilitated assaults. Limited previous experimental studies are available regarding the excretion of benzodiazepines in urine and oral fluid. The aim of this study was to investigate the concentrations of diazepam and alprazolam in oral fluid and urine for up to 2 weeks after ingestion of a single oral dose in healthy volunteers. A total of 11 healthy volunteers ingested 10 mg diazepam at the start of the study and 0...
December 5, 2018: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/30517195/rapid-and-highly-specific-generation-of-targeted-dna-sequencing-libraries-enabled-by-linking-capture-probes-with-universal-primers
#5
Joel Pel, Amy Leung, Wendy W Y Choi, Milenko Despotovic, W Lloyd Ung, Gosuke Shibahara, Laura Gelinas, Andre Marziali
Targeted Next Generation Sequencing (NGS) is being adopted increasingly broadly in many research, commercial and clinical settings. Currently used target capture methods, however, typically require complex and lengthy (sometimes multi-day) workflows that complicates their use in certain applications. In addition, small panels for high sequencing depth applications such as liquid biopsy typically have low on-target rates, resulting in unnecessarily high sequencing cost. We have developed a novel targeted sequencing library preparation method, named Linked Target Capture (LTC), which replaces typical multi-day target capture workflows with a single-day, combined 'target-capture-PCR' workflow...
2018: PloS One
https://www.readbyqxmd.com/read/30517183/exploiting-antigen-receptor-information-to-quantify-index-switching-in-single-cell-transcriptome-sequencing-experiments
#6
Ying Yao, Asima Zia, Łukasz Wyrożemski, Ida Lindeman, Geir Kjetil Sandve, Shuo-Wang Qiao
By offering high sequencing speed and ultra-high-throughput at a low price, Illumina next-generation sequencing platforms have been widely adopted in recent years. However, an experiment with multiplexed library could be at risk of molecular recombination, known as "index switching", which causes a proportion of the reads to be assigned to an incorrect sample. It is reported that a new advance, exclusion amplification (ExAmp) in conjunction with the patterned flow cell technology introduced on HiSeq 3000/HiSeq 4000/HiSeq X sequencing systems, potentially suffers from a higher rate of index switching than conventional bridge amplification...
2018: PloS One
https://www.readbyqxmd.com/read/30517099/preliminary-development-of-an-assay-for-detection-of-tert-expression-telomere-length-and-telomere-elongation-in-single-cells
#7
Ajay Ravindranathan, Beth Cimini, Morgan E Diolaiti, Bradley A Stohr
The telomerase enzyme enables unlimited proliferation of most human cancer cells by elongating telomeres and preventing replicative senescence. Despite the critical importance of telomerase in cancer biology, challenges detecting telomerase activity and expression in individual cells have hindered the ability to study patterns of telomerase expression and function across heterogeneous cell populations. While sensitive assays to ascertain telomerase expression and function exist, these approaches have proven difficult to implement at the single cell level...
2018: PloS One
https://www.readbyqxmd.com/read/30516988/restricted-access-volatile-supramolecular-solvents-for-single-step-extraction-cleanup-of-benzimidazole-anthelmintic-drugs-in-milk-prior-to-lc-ms-ms
#8
Francesca Accioni, Noelia Caballero-Casero, Diego García-Gómez, Soledad Rubio
In this work, a restricted access volatile supramolecular solvent (RAM-VOL-SUPRAS) directly synthesized in milk is proposed for the first time for the simultaneous extraction and cleanup of benzimidazole anthelmintic drugs in milk meant for human consumption. The RAM-VOL-SUPRAS was formed by the self-assembly and coacervation of hexanol in tetrahydrofuran induced by the water content in milk. Benzimidazoles legislated by the European Union were quantitatively extracted (80-110%) Proteins were precipitated by the action of THF and the amphiphile, extraction of carbohydrates was avoided by a size exclusion mechanism and lipids were removed during hexanol evaporation...
December 5, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/30516882/dna-methylation-analysis-of-selected-genes-for-the-detection-of-early-stage-lung-cancer-using-circulating-cell-free-dna
#9
Zhiping Yang, Weibo Qi, Li Sun, Hui Zhou, Biliu Zhou, Yi Hu
BACKGROUND: Lung cancer is still the deadliest cancer in the world, but early diagnosis cannot be achieved because of the limitations of diagnostic methods. DNA methylation has been proven to be a potentially powerful tool for cancer detection and diagnosis over the past decade. OBJECTIVES: We explored whether free DNA methylation in plasma can be a reliable biomarker for noninvasive lung cancer detection. MATERIAL AND METHODS: We detected the methylation of 8 genes in plasma-free DNA of patients with pulmonary space-occupying lesions using real-time quantitative methylation-specific polymerase chain reaction (QMSP)...
December 5, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/30516836/vdr-rs7975232-apai-genetic-variation-predicts-sustained-hbsag-loss-in-hbeag-positive-chronic-hepatitis-b-patients-treated-with-pegylated-interferon
#10
Shan Ben, Wang Jun Yan, Wang Xia, Fu Juan Juan, Li Li, Pan Xiu Cheng, Li Jian Jun, Tang Xian Tuan
AIM: To evaluate the predictive value of vitamin D and its metabolic pathway gene polymorphisms in response to pegylated interferon (Peg-IFN) in HBeAg-positive CHB patients. METHODS: 119 HBeAg-positive CHB patients who received Peg-IFN monotherapy for 48 weeks and then were followed-up for another 48 weeks were prospectively enrolled; baseline 25-(OH)D and HBV serologic marker levels were detected, 9 critical single nucleotide polymorphisms within vitamin D metabolism were genotyped...
December 5, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/30516819/potential-role-of-ocular-microbiome-host-genotype-tear-cytokines-and-environmental-factors-in-corneal-infiltrative-events-in-contact-lens-wearers
#11
Cecilia Chao, Lakshmi Akileswaran, Jessica N Cooke Bailey, Mark Willcox, Russell Van Gelder, Carol Lakkis, Fiona Stapleton, Kathryn Richdale
Purpose: The purpose of this study was to explore differences in genotype, ocular surface microbiome, tear inflammatory markers, and environmental and behavioral exposures in soft contact lens (SCL) wearers with and without a history of corneal infiltrative events (CIEs). Methods: Nine SCL wearers with a recent CIE and nine age-, sex-, and SCL material- and modality-matched controls were enrolled. The Contact Lens Risk Survey, slit-lamp examination data, basal tears, conjunctival microbial cultures, and peripheral blood samples were collected...
December 3, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/30516689/fdg-pet-ct-for-detecting-an-infection-focus-in-patients-with-bloodstream-infection-factors-affecting-diagnostic-yield
#12
Jordy P Pijl, Andor W J M Glaudemans, Riemer H J A Slart, Derya Yakar, Marjan Wouthuyzen-Bakker, Thomas C Kwee
PURPOSE: To investigate the diagnostic performance of F-fluoro-2-deoxy-D-glucose (FDG) PET/ CT for the detection of an infection focus in patients with a bloodstream infection (BSI) and to identify factors influencing the diagnostic yield of FDG-PET/CT. METHODS: This retrospective single-center study included 185 consecutive patients with a BSI who underwent an FDG-PET/CT scan for the detection of an infection focus between 2010 and 2017. The final diagnosis at hospital discharge was used as reference standard...
December 3, 2018: Clinical Nuclear Medicine
https://www.readbyqxmd.com/read/30516199/a-benzimidazolyl-terpyridine-fe-2-system-and-its-recognition-driven-molecular-model-of-a-traffic-light
#13
Avinash Kumar Sonkar, Abhishek Rai, Kamini Tripathi, Pooja Sharma, H W Roesky, Michael G B Drew, Lallan Mishra
A new benzimidazolyl terpyridyl ligand (BIT) selectively detects Fe2+, F- and CN- ions in acetonitrile. The Fe2+ complex [Fe(H-BIT)2]·(ClO4)4 (1) displays the fast and selective detection of 2,4,6-trinitrophenol (TNP) with strong binding (log β = 8.51 ± 0.24) and a low limit of detection (1.4 × 10-7 M) among several nitroaromatics. The color of complex 1 changes from purple to red, yellow, and green upon the separate addition of 2,4,6-trinitrophenol and tetrabutylammonium salts of fluoride and cyanide, respectively...
December 5, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/30516182/a-rolling-circle-amplification-assisted-dna-walker-triggered-by-multiple-dnazyme-cores-for-highly-sensitive-electrochemical-biosensing
#14
Sina Wang, Yuhang Ji, Haomin Fu, Huangxian Ju, Jianping Lei
DNA walkers from monopodial to multipedal types have usually one cleavage site to power the walking system along with the track. Herein, a multipedal DNA walker (m-DNA walker) with multiple DNAzyme cores was constructed with the assistance of rolling circle amplification (RCA) for highly sensitive electrochemical biosensing. Firstly, a three-component DNA complex as a swing strand was prepared by integrating a padlock, an RCA primer and a block DNA as a recognition element in the DNA walker system. After ferrocene-labeled track DNA (trDNA) and capture DNA were fixed on a gold electrode, the three-component DNA complex was imported onto the electrode as a swing arm to form a m-DNA walker...
December 5, 2018: Analyst
https://www.readbyqxmd.com/read/30516050/exploitation-of-the-flexidentate-nature-of-a-ligand-to-synthesize-zn-ii-complexes-of-diverse-nuclearity-and-their-use-in-solid-state-naked-eye-detection-and-aqueous-phase-sensing-of-2-4-6-trinitrophenol
#15
Tanmoy Kumar Ghosh, Subrata Jana, Ashutosh Ghosh
Three Zn(II) complexes, [Zn2 (HL)2 (NO3 )2 ]·H2 O (1), [(Zn4 L2 )(μ3 -OH)2 ](NO3 )2 ·0.5H2 O (2), and [(Zn6 L2 )( o-van)2 (μ3 -OCH3 )2 (μ3 -OH)2 ](NO3 )2 (3), have been synthesized by exploiting the flexidentate nature of a multidentate Schiff base ligand, H2 L ( N, N'-bis(3-methoxysalicylidene)diethylenetriamine), by changing the reaction conditions and stoichiometry of the reactants. All three complexes are highly fluorescent in solution as well as in solid and have been used as luminescence sensors toward nitrophenol explosives in both the media...
December 5, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/30515928/donor-specific-anti-hla-antibody-production-following-pediatric-abo-incompatible-heart-transplantation
#16
Chiu-Yu Chen, Paul Warner, Erin L Albers, Mariska S Kemna, Meghan Delaney, Borah J Hong, Yuk M Law
ABO-i heart transplantation can be performed in infants with end-stage heart failure to increase organ availability. The development of newly detected DSAs is associated with decreased cardiac graft survival, and the effect of ABO-i transplantation on DSA production is unknown. We examined DSA production and rejection frequency in infant recipients of ABO-i and ABO-c heart transplants via a retrospective cohort study of infant heart transplant recipients transplanted at a single pediatric center between January 2004 and November 2014...
December 4, 2018: Pediatric Transplantation
https://www.readbyqxmd.com/read/30515742/single-stage-anterior-debridement-posterior-instrumentation-and-global-fusion-in-thoracic-and-thoracolumbar-tubercular-spondylodiscitis
#17
S P Mohanty, M Pai Kanhangad, B Yogesh Kumar, A Singh
PURPOSE: The present study evaluated the clinical and radiological outcomes in patients with tuberculosis of the thoracic and thoracolumbar spine, treated by an operative technique in which anterior debridement with posterior instrumentation and global fusion was performed through a single-stage posterior approach. METHODS: Ninty-seven patients with spinal tuberculosis, between T1 and L1, in whom anterior debridement, bone grafting, with posterior instrumentation and fusion was performed through a single-stage posterior approach, with a minimum follow-up of 2 years, formed the basis of this study...
December 4, 2018: Musculoskeletal Surgery
https://www.readbyqxmd.com/read/30515677/glycemic-variation-in-uncontrolled-graves-disease-patients-with-normal-glucose-metabolism-assessment-by-continuous-glucose-monitoring
#18
Gu Gao, Feng-Fei Li, Yun Hu, Reng-Na Yan, Bing-Li Liu, Xiao-Mei Liu, Xiao-Fei Su, Jian-Hua Ma, Gang Hu
PURPOSE: To observe the glycemic variation (GV) in uncontrolled Graves' disease (GD) patients with normal glucose metabolism measured by continuous glucose monitoring (CGM). METHODS: This was a single-center, open-label, observational study. From January 2017 to October 2017, 20 GD patients with normal glucose metabolism and 24 healthy control subjects were recruited. Serum samples were obtained at 0, 30, and 120 min after oral glucose loading for glucose, insulin, and C-peptide level measurements...
December 4, 2018: Endocrine
https://www.readbyqxmd.com/read/30515332/video-capsule-endoscopy-in-the-assessment-of-portal-hypertensive-enteropathy
#19
Yasir Al-Azzawi, Lidia Spaho, Mohammed Mahmoud, Joan Kheder, Anne Foley, David Cave
Background: The features of the portal hypertension enteropathy (PHE) vary from mild mucosal changes to varices with or without bleeding. The prevalence and the development are not fully understood. Aim: Our aim is to examine the prevalence and the different manifestations of PHE using video capsule endoscopy (VCE). Methods: It is a single center retrospective study of patients with cirrhosis, who had VCE. Based on the published literature, we divided the PHE lesions into vascular lesions and mucosal lesions...
2018: International Journal of Hepatology
https://www.readbyqxmd.com/read/30515031/the-availability-of-echinococcus-igg-elisa-for-diagnosing-pulmonary-hydatid-cysts
#20
Yener Aydin, Bayram Altuntas, Abdulkadir Kaya, Ali Bilal Ulas, Muhammet Hamidullah Uyanık, Atilla Eroglu
Objective: In this study, we researched the availability of Echinococcus IgG ELISA in pulmonary hydatid cysts. Materials and Methods: Between January 2008 and December 2015, 93 successive cases, which were studied in preoperative Echinococcus IgG and histopathologically found to have pulmonary hydatid cysts, were retrospectively analyzed. Age and sex of the cases and the cyst's location, number, size, spread to other organs outside the lungs, and its condition as intact or ruptured were reviewed...
October 2018: Eurasian Journal of Medicine
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