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Colorectal Cancer And MiRNA

Pengfei Qiao, Shenglong Li, Haogang Zhang, Lei Yao, Fujing Wang
Colorectal cancer (CRC) is among the most common malignancies of the digestive system. Dysregulation of miRNAs and the farnesoid X receptor (FXR) are involved in the progression of CRC. In the present study, the effects of FXR and miR‑135A1 in CRC were evaluated. Reverse transcription quantitative‑polymerase chain reaction (RT‑qPCR) was used to examine the expression of miR‑135A1 in patient CRC tissues and adjacent non‑tumor tissues, as well as cell lines. The association between miR‑135A1 and clinical characteristics of patients with CRC was also investigated...
August 7, 2018: Oncology Reports
Na Zhou, Zhao Sun, Ningning Li, Yuping Ge, Jianfeng Zhou, Qin Han, Lin Zhao, Chunmei Bai
The incidence and mortality of colorectal cancer (CRC) have been rising rapidly in China. A number of miRNAs have been confirmed to be involved in diverse biological processes of CRC. However, whether miR‑197 plays a role in migration and invasion of CRC has never been explored. In the present study Transwell chambers were used in in vitro migration and invasion assays. Dual-luciferase reporter assay was employed to confirm the target of miR‑197. RT‑PCR and IHC staining were performed to quantify miR‑197 and IGFBP3 expression, respectively...
August 10, 2018: Oncology Reports
Shuaixi Yang, Zhenqiang Sun, Quanbo Zhou, Weiwei Wang, Guixian Wang, Junmin Song, Zhen Li, Zhiyong Zhang, Yuan Chang, Kunkun Xia, Jinbo Liu, Weitang Yuan
Noncoding RNAs (ncRNAs) can be divided into microRNAs (miRNAs), long noncoding RNAs (lncRNAs), circular RNAs (circRNAs), pRNAs, and tRNAs. Traditionally, miRNAs exert their biological function mainly through the inhibition of translation via the induction of target RNA transcript degradation. lncRNAs and circRNAs were once considered to have no potential to code proteins. Here, we will review the current knowledge on ncRNAs in relation to their origins, characteristics, and functions. We will also review how ncRNAs work as competitive endogenous RNA, gene transcription and expression regulators, and RNA-binding protein sponges in colorectal cancer (CRC)...
2018: Cancer Management and Research
Xiu-Xiu Hu, Xue-Ni Xu, Bang-Shun He, Hui-Ling Sun, Tao Xu, Xiang-Xiang Liu, Xiao-Xiang Chen, Kai-Xuan Zeng, Shu-Kui Wang, Yu-Qin Pan
Colorectal cancer(CRC) is a prevalent malignancy in the world. There is growing evidence that microRNAs (miRNAs) as crucial modulator are in connection with many tumor-related diseases including CRC. Though miR-485-5p has been reported as an anti-oncogene in certain cancers, it remains unclear in CRC. In this research, we found that miR-485-5p was at lower level expression in CRC tissues and cell lines compared to the paired paracancerous tissues and the normal colon epithelial cell line FHC, correspondingly...
2018: Journal of Cancer
Yang Li, Yanhong Duo, Peng Zhai, Lisheng He, Keli Zhong, Yue Zhang, Kaibin Huang, Jinfeng Luo, Han Zhang, Xiaofang Yu
AIM: We aim to explore the regulatory mechanism of miR-328 and further develop miR-328-loaded mesoporous silica nanoparticles (MSNs) and surface-decorated with polymerized dopamine, epithelial cell adhesion molecule aptamer and bevacizumab for the dual-targeting treatment of colorectal cancer (CRC). MATERIALS & METHODS: The relationship between miR-328 and CPTP and the mechanism and antitumor effect of MSNs-miR-328@PDA-PEG-Apt-Bev were evaluated. RESULTS: We found CPTP is a direct target of miR-328...
August 7, 2018: Nanomedicine
Feng He, Zhi Song, Huacui Chen, Zhuanpeng Chen, Ping Yang, Wanglin Li, Zhi Yang, Tong Zhang, Fei Wang, Jianchang Wei, Fang Wei, Qiang Wang, Jie Cao
Long noncoding RNAs (lncRNAs) are implicated in human cancer, but their mechanisms of action are largely unknown. In this study, we investigated lncRNA alterations that contribute to colorectal cancer (CRC) through microarray expression profiling in CRC patient samples. Here, we report that the CRC-associated lncRNA PVT1-214 is a key regulator of CRC development and progression; patients with high PVT1-214 expression had a shorter survival and poorer prognosis. In vitro and in vivo investigation of the role of PVT1-214 revealed a complex integrated phenotype affecting cell growth, stem-like properties, migration, and invasion...
August 3, 2018: Oncogene
Jeyalakshmi Kandhavelu, Kumar Subramanian, Amber Khan, Aadilah Omar, Paul Ruff, Clement Penny
BACKGROUND: Globally, colorectal cancer (CRC) is the third most common cancer in women and the fourth most common cancer in men. Dysregulation of small non-coding miRNAs have been correlated with colon cancer progression. Since there are increasing reports of candidate miRNAs as potential biomarkers for CRC, this makes it important to explore common miRNA biomarkers for colon cancer. As computational prediction of miRNA targets is a critical initial step in identifying miRNA:mRNA target interactions for validation, we aim here to construct a potential miRNA network and its gene targets for colon cancer from previously reported candidate miRNAs, inclusive of 10 up- and 9 down-regulated miRNAs from tissues; and 10 circulatory miRNAs...
August 2, 2018: MicroRNA
Anna Prossomariti, Giulia Piazzi, Leonarda D'Angelo, Sara Miccoli, Daniela Turchetti, Chiara Alquati, Claudio Montagna, Franco Bazzoli, Luigi Ricciardiello
Adenomatous polyposis coli (APC) gene mutations are responsible for the onset of Familial Adenomatous Polyposis (FAP) and sporadic colorectal cancer (CRC) and have been associated with microRNA (miRNA) dysregulation. The capacity of miR-155, a cancer-related miRNA, to target components of the Wnt/β-catenin pathway suggests that APC gene mutations, controlling miRNA expression, may critically regulate Wnt/β-catenin signaling. To this end, APC gene target sequencing was performed on colonic adenomatous polyps and paired normal mucosa clinical specimens from FAP patients (n=9) to elucidate the role of miR-155-5p in APC-mutant setting...
August 2, 2018: Molecular Cancer Research: MCR
Bomi Kwak, Dong Uk Kim, Tae Oh Kim, Heui-Soo Kim, Sang-Woo Kim
The aberrant expression of microRNAs (miRNAs or miRs) has been shown to be associated with the development of various types of cancer, including colorectal cancer (CRC). The increased activation of the Wnt signaling pathway via the loss of the Wnt repressor, adenomatous polyposis coli (APC), is the hallmark of human CRC. In this study, we demonstrate that the activation of the Wnt/c-Myc axis inhibits the expression of the tumor suppressor, p53, via promoting the targeting of p53 by miR‑552. Our results revealed that the ectopic expression of miR‑552 enhanced cell proliferation, colony formation and resistance to drug-induced apoptosis, suggesting that this miRNA may function as an oncogene...
July 26, 2018: International Journal of Oncology
D-S Wang, B Zhong, M-S Zhang, Y Gao
OBJECTIVE: Circulating microRNAs (miRNAs) have been reported as biomarkers for the early detection of colorectal cancer (CRC). We aimed at evaluating the diagnostic and prognostic value of serum miR-103 in CRC patients. PATIENTS AND METHODS: Quantitative reverse-transcription PCR (qRT-PCR) was applied to measure the miR-103 levels in blood samples of 96 patients and 60 controls. RESULTS: Our results demonstrated that serum miR-103 was overexpressed in CRC subjects and the receiver operating characteristic (ROC) curve analysis showed that serum miR-103 could differentiate CRC cases from controls with relatively high accuracy...
July 2018: European Review for Medical and Pharmacological Sciences
Saeid Afshar, Sepideh Afshar, Emily Warden, Hamed Manochehri, Massoud Saidijam
Background: The early diagnosis of colorectal cancer (CRC) is associated with improved survival rates, and development of novel non-invasive, sensitive, and specific diagnostic tests is highly demanded. The objective of this paper was to identify commonly circulating microRNA (miRNA) biomarkers for use in CRC diagnosis. Methods: For this purpose, an artificial neural network (ANN) model was proposed. Among miRNAs retrieved from the Gene Expression Omnibus dataset, four miRNAs with the best miRNA score were selected by ANN units...
July 30, 2018: Iranian Biomedical Journal
Fang Wu, Fang Liu, Lemei Dong, Han Yang, Xixi He, Lili Li, Lihao Zhao, Sisi Jin, Gang Li
AMP-activated protein kinase (AMPK) is a metabolic regulator that acts to limit the growth of cancer cells. AMPK is downregulated by melanoma antigens A3/6 (MAGEA3/6), which are cancer-specific proteins that enhance the activity of specific E3 ubiquitin ligases to ubiquitinate and degrade AMP-activated protein kinase α1 (AMPKα1). Here, using a bioinformatic approach, we identified a microRNA, miR-1273 g-3p, that is predicted to target the 3' untranslated region (UTR) of MAGEA3/6. Analyzing miR-1273 g-3p expression in human colon cancer tissues, we found a reduction in miR-1273 g-3p expression correlating with increased MAGEA3/6 expression and AMPKα1 downregulation...
July 26, 2018: Cancer Letters
Nicola Normanno, Andres Cervantes, Fortunato Ciardiello, Antonella De Luca, Carmine Pinto
The term liquid biopsy refers to the analysis of biomarkers in any body fluid, including blood, urine and cerebrospinal fluid. In cancer, liquid biopsy testing allows the analysis of tumor-derived DNA, RNA, miRNA and proteins that can be either cell-free or contained in circulating tumor cells (CTC), extracellular vesicles (EVs) or platelets. A number of studies suggest that liquid biopsy testing could have a relevant role in the management of colorectal cancer (CRC) patients at different stages of the disease...
July 18, 2018: Cancer Treatment Reviews
Kenneth Kw To, Christy Ws Tong, Mingxia Wu, William Cs Cho
MicroRNAs (miRNAs) are small, single-stranded, noncoding RNAs that can post-transcriptionally regulate the expression of various oncogenes and tumor suppressor genes. Dysregulated expression of many miRNAs have been shown to mediate the signaling pathways critical in the multistep carcinogenesis of colorectal cancer (CRC). MiRNAs are stable and protected from RNase-mediated degradation, thereby enabling its detection in biological fluids and archival tissues for biomarker studies. This review focuses on the role and application of miRNAs in the prognosis and therapy of CRC...
July 21, 2018: World Journal of Gastroenterology: WJG
Youmao Tao, Chong Ma, Qihao Fan, Yannan Wang, Tao Han, Caixia Sun
MicroRNAs (miRNAs) are involved in cancer genesis and progression via acting as tumor suppressors or oncogenes. Previous studies report that miR-1296 shows upregulation in both colorectal cancer (CRC) tissues and plasma samples. However, the accurate clinical significance of miR-1296 and its role in CRC have not been well investigated. The aim of the present study was to disclose the aberrant expression, clinical significance, and the relevant biological function of miR-1296 in CRC. We found a marked upregulation of miR-1296 expression in CRC tissues compared to tumor-adjacent tissues...
2018: Journal of Cancer
Michele Sook Yuin Hiew, Han Ping Cheng, Chiu-Jung Huang, Kowit Yu Chong, Soon Keng Cheong, Kong Bung Choo, Tunku Kamarul
BACKGROUND: Induced pluripotency in cancer cells by ectopic expression of pluripotency-regulating factors may be used for disease modeling of cancers. MicroRNAs (miRNAs) are negative regulators of gene expression that play important role in reprogramming somatic cells. However, studies on the miRNA expression profile and the expression patterns of the mesenchymal-epithelial transition (MET)/epithelial-mesenchymal transition (EMT) genes in induced pluripotent cancer (iPC) cells are lacking...
July 19, 2018: Journal of Biomedical Science
Michihisa Iida, Shoichi Hazama, Ryouichi Tsunedomi, Hironori Tanaka, Hiroko Takenouchi, Shinsuke Kanekiyo, Yukio Tokumitsu, Shinobu Tomochika, Yoshihiro Tokuhisa, Kazuhiko Sakamoto, Nobuaki Suzuki, Shigeru Takeda, Tomio Ueno, Shigeru Yamamoto, Shigefumi Yoshino, Koji Fujita, Masahiko Kuroda, Hiroaki Nagano
The cancer stroma is important in cancer development, however, whether the aberrant expression of microRNAs (miRNAs) in the cancer stroma is associated with cancer progression remains to be fully elucidated. The aim of the present study was to identify the miRNAs associated with liver metastasis in the cancer stroma of human colorectal cancer (CRC). Using laser capture microdissection, cancer stroma was obtained from the primary lesion of six patients with CRC with liver metastasis (CRCwLM) and six patients with CRC without liver metastasis (CRCwoLM), and miRNA microarray analysis was performed...
September 2018: Oncology Reports
Yun Feng, Yu-Wei Dong, Yun-Na Song, Jun-Hua Xiao, Xing-Ya Guo, Wei-Liang Jiang, Lun-Gen Lu
An early diagnosis of colitis‑associated colorectal cancer (CAC) is important for its clinical management. However, it is currently difficult to distinguish the different stages of CAC development. MicroRNA dysregulation is common in human colorectal disorders, however little is known regarding whether miRNA affects tumor progression by regulating inflammation. In the present study, we identified a novel miRNA (miR‑449a), the expression of which was significantly reduced in CAC tissues than in paired adjacent non‑cancerous tissues (ANTs)...
September 2018: Oncology Reports
Yan Qin, Xiao Chen, Zhihu Liu, Xiaopeng Tian, Zhibin Huo
BACKGROUND 5-Fluorouracil (5-FU)-based chemotherapy is a conventional therapeutic approach for the treatment of patients with colorectal cancer (CRC). However, development of 5-FU resistance frequently occurs. We explored a potential method for regulating the sensitivity to 5-FU-based chemotherapy in CRC patients. MATERIAL AND METHODS Cell viability was determined by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Gene expression levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR)...
July 16, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
María Teresa Corcuera, Cruz Rodríguez-Bobada, Jaime Zuloaga, Fernando Gómez-Aguado, Rosa Rodríguez-Perez, Ángel Mendizabal, Pablo González, Javier Arias-Díaz, María Luisa Caballero
Anisakiasis is a global disease caused by consumption of raw or lightly cooked fish parasitised with Anisakis spp. third-stage larvae. Cases in the literature show colocalised anisakiasis and colorectal cancer, and the incidental finding of Anisakis larvae at the tumour site was reported. Data from our group suggested an epidemiological link between previous infection and gastrointestinal cancer. Furthermore, it has recently been reported that Anisakis products lead to inflammation and DNA damage. Based on these facts, the aim was to investigate whether Anisakis antigens are able to induce changes in the proliferation of epithelial cells in vitro or in the expression of serum microRNA (miRNA) in Sprague-Dawley rats...
July 13, 2018: Parasitology Research
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