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Colorectal Cancer And MiRNA

Taicheng Zhou, Lili Wu, Qirui Wang, Zhipeng Jiang, Yingru Li, Ning Ma, Wenhao Chen, Zehui Hou, Wenchang Gan, Shuang Chen
Colorectal cancer (CRC) is a malignancy with high metastatic rates. The mechanism of miR-128 on the regulation of Ribophorin-II (RPN2) in CRC cells was explored in the present study. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) or western blot analyses were conducted to detect miR-128 and RPN2 levels in tissues and cell lines. AmiR-128 overexpression model was constructed using miR-128 mimic transfection in HT29 CRC cells. Then, cell proliferation was detected using a Cell Counting Kit-8 assay, and the migratory and invasive abilities were measured by Transwell assay...
December 2018: Oncology Letters
Carolyn M Klinge
Non-coding RNAs (ncRNAs) are regulators of intracellular and intercellular signaling in breast cancer. ncRNAs modulate intracellular signaling to control diverse cellular processes, including levels and activity of estrogen receptor α (ERα), proliferation, invasion, migration, apoptosis, and stemness. In addition, ncRNAs can be packaged into exosomes to provide intercellular communication by the transmission of microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) to cells locally or systemically. This review provides an overview of the biogenesis and roles of ncRNAs: small nucleolar RNA (snRNA), circular RNAs (circRNAs), PIWI-interacting RNAs (piRNAs), miRNAs, and lncRNAs in breast cancer...
December 12, 2018: Non-Coding RNA
Tao Zhang, Jianrong Guo, Jian Gu, Zheng Wang, Guobin Wang, Huili Li, Jiliang Wang
Colorectal cancer (CRC) is one of the principal causes of cancer‑associated mortality worldwide. The high incidence of liver metastasis is the leading risk factor of mortality in patients with CRC, and the mechanisms of CRC liver metastasis are poorly understood. In the present study, 7 datasets, including 3 gene expression profile datasets and 4 microRNA (miRNA) expression profile datasets were downloaded from the NCBI Gene Expression Omnibus (GEO) database to identify potential key genes and miRNAs, which may be candidate biomarkers for CRC liver metastasis...
November 1, 2018: Oncology Reports
Fangyao Chen, Zhe Li, Hui Zhou
OBJECT: This study was conducted to identify the prognostic microRNA (miRNA) for the prediction of survival in colon adenocarcinoma (CA). METHODS: miRNA profiling of patients with CA was downloaded from The Cancer Genome Atlas (TCGA) database. After data processing, univariate Cox regression was performed to select potential prognostic miRNAs. Least absolute shrinkage and selection operator approach was conducted to identify the key prognostic miRNA biomarkers. Log-rank test was performed to compare survival outcome between patients with different regulation type of identified miRNAs...
December 9, 2018: Journal of Cellular Biochemistry
Z-Y Liu, L Yang, H-Y Chang
OBJECTIVE: MicroRNAs (miRNAs) as a new class of biomarkers have been explored in recent studies. We investigate whether miR-1256 could be considered as powerful biomarkers for predicting the prognosis of colorectal cancer (CRC). PATIENTS AND METHODS: The expression of miR-1256 in CRC was compared with matched normal tissue and using qRT-PCR. The correlation of miR-1256 expression with clinicopathological factors was statistically analyzed. Survival rate was determined with Kaplan-Meier and statistically analyzed with the log-rank method between groups...
November 2018: European Review for Medical and Pharmacological Sciences
Yiping Zhu, Yinzhu Bian, Qun Zhang, Jing Hu, Li Li, Mi Yang, Hanqing Qian, Lixia Yu, Baorui Liu, Xiaoping Qian
Colorectal cancer (CRC) is one of the most frequently diagnosed digestive system cancer. The aim of the present study was to investigate the interactions among messenger RNAs (mRNAs), microRNAs (miRNAs), and long noncoding RNAs (lncRNAs) in CRC to reveal the mechanisms of CRC. Differentially expressed genes (DEGs) were identified from public gene expression data sets. One thousand eighty-one common dysregulated mRNAs in two data sets were identified. Gene function analysis and protein-protein interaction network analysis indicated that these DEGs might play important roles in CRC...
December 7, 2018: Journal of Cellular Biochemistry
Pouria Samadi, Saeid Afshar, Razieh Amini, Rezvan Najafi, Ali Mahdavinezhad, Abdolazim Sedighi Pashaki, Mohammad Hadi Gholami, Massoud Saidijam
Abnormal expression of various microRNAs (miRNAs), as regulators of biological signaling pathways, has a strong association with cancer resistance to chemotherapy and radiotherapy. The let-7 family of miRNAs as tumor suppressors have shown to be downregulated in different types of human malignancies including colorectal cancer (CRC). However, the biological function of let-7 members in the processes of resistance to radiation in CRC has not yet been completely elucidated. Insulin-like growth factor 1 receptor (IGF-1R) signaling pathway is amplified in CRC and leads to its progression, development, and also radiation resistance...
December 4, 2018: Journal of Cellular Physiology
Xiangyu Zhu, Si-Ping Ma, Dongxiang Yang, Yanlong Liu, Yong-Peng Wang, Tao Lin, Yan-Xi Li, Shi-Hua Yang, Wan-Chuan Zhang, Xin-Ling Wang
BACKGROUND/AIMS: Deregulation of microRNAs (miRNAs) has been associated with a variety of cancers, including colorectal cancer (CRC). Here, we investigated anomalous miR-142-3p expression and its possible functional consequences in primary CRC samples. METHODS: The expression of miR-142-3p was measured by quantitative RT-PCR in 116 primary CRC tissues and adjacent non-tumor tissues. The effect of miR-142-3p up- or down-regulation in CRC-derived cells was evaluated in vitro by cell viability and colony formation assays and in vivo by growth assays in xenografted nude mice...
December 4, 2018: Cellular Physiology and Biochemistry
B B Du, T Wang, X F Yang, J K Wang, X L Shi, X Y Wang, D W Wu, L L Feng, L J Chen, W S Zhang
The study aimed to identify chemoradiotherapy (CRT)-sensitive biomarkers in colorectal cancer (CRC) patients. The GSE15781 dataset used in this study contains 42 samples: 22 CRC tissues (non-CRT: n = 13; CRT: n = 9) and 20 normal colorectal tissues (non-CRT: n = 10; CRT: n = 10). Following pretreatment, differentially expressed genes were selected using the limma package. Potential CRT-sensitive genes were identified with Venn analysis and then enriched in function and pathway clusters using the DAVID online tool...
August 9, 2018: Neoplasma
Naghmeh Shirafkan, Navid Shomali, Tohid Kazemi, Dariush Shanehbandi, Mehri Ghasabi, Elham Baghbani, Maziar Ganji, Vahid Khaze, Behzad Mansoori, Behzad Baradaran
PURPOSE: Altered expression of microRNAs (miRNAs) is indicated strongly in colorectal cancer (CRC). This study aims to evaluate the inhibitory role of miR-193a-5p on epithelial-mesenchymal transition markers in CRC lines. The cellular effects and potential mechanisms of miR-193a-5p were also examined. METHODS: Quantitative reverse-transcription polymerase chain reaction (RT-PCR) was performed to determine the expression of miR-193a-5p in three CRC cell lines (HCT-116, SW-480, and HT-29) and its impact on metastasis-related genes ( vimentin and CXCR4) before and after mimic transfection...
December 2, 2018: Journal of Cellular Biochemistry
Wenliang Yuan, Xiaobo Li, Li Liu, Cai Wei, Dan Sun, Sihua Peng, Linhua Jiang
Colorectal cancer (CRC) is one of the most common malignancies and morbidity and mortality are increasing rapidly. Increasing evidence showed the close correlation between aberrant expression of certain RNAs and the occurrence and development of CRC. However, comprehensive analyses of differentially expressed profiles of linRNA in CRC based on large sample size have been lacking. In the present study, based on RNA-seq data obtained from the TCGA (The Cancer Genome Atlas) database, we identified 1176 lncRNAs, 245 miRNAs and 2083 mRNAs whichaberrantly expressed in the colorectal cancer tissues compared with the adjacent non-tumorous tissues...
November 28, 2018: Biochemical and Biophysical Research Communications
Mostafa Sarvizadeh, Ziba V Malekshahi, Ebrahim Razi, Hossein Sharifi, Nousin Moussavi, Mohsen Taghizadeh
Colorectal cancer (CRC) is one of the important malignancies that result in cancer-related deaths worldwide. Multiple lines of evidence have indicated that different responses to therapy in CRC cells led to the failure of the current therapies. Hence, identification of the underlying cellular and molecular pathways involved in the emergence of different responses from CRC cells could contribute to finding and designing new therapeutic platforms to overcome the present limitations. Among the various targets involved in CRC pathogenesis, microRNAs (miRNAs) have key roles in many signaling pathways that are associated with the initiation and progression of CRC...
November 27, 2018: Journal of Cellular Physiology
Ping Xuan, Yihua Dong, Yahong Guo, Tiangang Zhang, Yong Liu
Identification of disease-related microRNAs (disease miRNAs) is helpful for understanding and exploring the etiology and pathogenesis of diseases. Most of recent methods predict disease miRNAs by integrating the similarities and associations of miRNAs and diseases. However, these methods fail to learn the deep features of the miRNA similarities, the disease similarities, and the miRNA⁻disease associations. We propose a dual convolutional neural network-based method for predicting candidate disease miRNAs and refer to it as CNNDMP...
November 23, 2018: International Journal of Molecular Sciences
Volker Ast, Theresa Kordaß, Marcus Oswald, Amol Kolte, David Eisel, Wolfram Osen, Stefan B Eichmüller, Alexander Berndt, Rainer König
Colorectal cancer remains a leading cause of cancer-related death worldwide. A previous transcriptomics based study characterized molecular subgroups of which the stromal subgroup was associated with the worst clinical outcome. Micro-RNAs (miRNAs) are well-known regulators of gene expression and can follow a non-linear repression mechanism. We set up a model combining piecewise linear and linear regression and applied this combined regression model to a comprehensive colon adenocarcinoma dataset. We identified miRNAs involved in regulating characteristic gene sets, particularly extracellular matrix remodeling in the stromal subgroup...
October 30, 2018: Oncotarget
Ovidiu Balacescu, Daniel Sur, Calin Cainap, Simona Visan, Daniel Cruceriu, Roberta Manzat-Saplacan, Mihai-Stefan Muresan, Loredana Balacescu, Cosmin Lisencu, Alexandru Irimie
Colorectal cancer (CRC) is one of the most commonly diagnosed malignancies with a high incidence and mortality rate. An essential challenge in colorectal cancer management is to identify new prognostic factors that could better estimate the evolution and treatment responses of this disease. Considering their role in cancer development, progression and metastasis, miRNAs have become an important class of molecules suitable for cancer biomarkers discovery. We performed a systematic search of studies investigating the role of miRNAs in colorectal progression and liver metastasis published until October 2018...
November 22, 2018: International Journal of Molecular Sciences
Sujeong Park, Jinjoo Oh, Minhee Kim, Eun-Jung Jin
Here, we investigated the possible anti-cancer properties of bromelain in Kras mutant human colorectal carcinoma cell lines and a mouse model harboring a Kras mutation. Cell growth and proliferation were significantly reduced in the Kras mutant colorectal carcinoma cell lines following treatment with 50 μg/mL bromelain as assessed by crystal violet staining and a proliferation assay. To identify the molecules responsible for this action, the expression levels of genes involved in signaling pathways and miRNAs were analyzed by real-time PCR...
2018: Animal Cells and Systems
Huo Zhang, Mingxia Zhu, Xia Shan, Xin Zhou, Tongshan Wang, Jinying Zhang, Jinsong Tao, Wenfang Cheng, Gang Chen, Jian Li, Ping Liu, Qiang Wang, Wei Zhu
Colorectal cancer (CRC) has been one of the most commonly diagnosed cancers in global. The differential expression profiles of microRNAs (miRNAs) in CRC plasma of patients have the potential to serve as a diagnostic biomarker. We conducted a four-stage study to identify the potential plasma miRNAs for CRC detection. In the initial screening phase, Exiqon panel (miRCURY-Ready-to-Use-PCR-Human-panel-I + II-V1.M) including 3 CRC pools and 1 normal controls (NCs) pool were applied to acquire miRNA profiles...
November 17, 2018: Gene
Gaiqin Li, Yongtao Xu, Shishu Wang, Wenju Yan, Qin Zhao, Jianqiang Guo
Recent studies have demonstrated that dysregulation of mircoRNAs (miRNAs) greatly affected biological processes of human cancers, including colorectal cancer. As a member of miRNAs family, miR-873-5p has been proved to be a tumor suppressor in some human cancers. Here, we aim to investigate the effects of miR-873-5p on the migration, invasion and epithelial-mesenchymal transition (EMT) of colorectal cancer cells. The low expression of miR-873-5p in colorectal cancer cells was identified by conducting qRT-PCR analysis...
October 19, 2018: Pathology, Research and Practice
Sabine François, Benoit Usunier, Marie-Elisabeth Forgue-Lafitte, Bruno L'Homme, Marc Benderitter, Luc Douay, Norbert-Claude Gorin, Annette K Larsen, Alain Chapel
We here determine the influence of mesenchymal stem cell (MSC) therapy on the progression of solid tumors. The influence of MSCs was investigated in human colorectal cancer cells as well as in an immunocompetent rat model of colorectal carcinogenesis representative of the human pathology. Treatment with bone marrow (BM)-derived MSCs significantly reduced both cancer initiation and cancer progression by increasing the number of tumor-free animals as well as decreasing the number and the size of the tumors by half, thereby extending their lifespan...
November 19, 2018: Stem Cells Translational Medicine
Maryam Alidoust, Leila Hamzehzadeh, Mahdi Rivandi, Alireza Pasdar
Colorectal cancer (CRC) has been regarded as a common cancer due to its prevailing incidence in both males and females. Recently, non-coding RNAs used as biomarkers for screening, diagnosis and prognosis of different cancers have been under the focus of attention. As a result of this, the aim of this study was to systematically review articles that investigated the SNPs in genes related to microRNAs and long non-coding RNAs to assess the genetic susceptibility of colorectal cancer risk. The outcome is presented as the results of a meta-analysis...
December 2018: Critical Reviews in Oncology/hematology
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