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Colorectal Cancer And MiRNA

Ji-Su Mo, Seol-Hee Han, Ki-Jung Yun, Soo-Cheon Chae
OBJECTIVE AND DESIGN: MicroRNAs (miRNAs) play an important role in the pathogenesis of human diseases by regulating the expression of target genes in specific cells or tissues. In this study, we analyzed the association between the MIR429 and its target gene, charged multivesicular body protein 5 (CHMP5), in human colon cancer cells and in a DSS-induced colitis mouse model. MATERIALS AND METHODS: A luciferase reporter system was used to confirm the effect of MIR429 on CHMP5 expression...
October 17, 2018: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
Scott A Hinger, Diana J Cha, Jeffrey L Franklin, James N Higginbotham, Yongchao Dou, Jie Ping, Lihua Shu, Nripesh Prasad, Shawn Levy, Bing Zhang, Qi Liu, Alissa M Weaver, Robert J Coffey, James G Patton
The regulation and functional roles of secreted coding and long noncoding RNAs (lncRNAs; >200 nt) are largely unknown. We previously showed that mutant KRAS colorectal cancer (CRC) cells release extracellular vesicles (EVs) containing distinct proteomes, microRNAs (miRNAs), and circular RNAs. Here, we comprehensively identify diverse classes of CRC extracellular long RNAs secreted in EVs and demonstrate differential export of specific RNAs. Distinct noncoding RNAs, including antisense transcripts and transcripts derived from pseudogenes, are enriched in EVs compared to cellular profiles...
October 16, 2018: Cell Reports
Ailin Qu, Yongmei Yang, Xin Zhang, Wenfei Wang, Yingjie Liu, Guixi Zheng, Lutao Du, Chuanxin Wang
BACKGROUND: Preoperative prediction of lymph node (LN) status is of crucial importance for appropriate treatment planning in patients with colorectal cancer (CRC). In this study, we sought to develop and validate a non-invasive nomogram model to preoperatively predict LN metastasis in CRC. METHODS: Development of the nomogram entailed three subsequent stages with specific patient sets. In the discovery set (n = 20), LN-status-related miRNAs were screened from high-throughput sequencing data of human CRC serum samples...
October 9, 2018: EBioMedicine
Yang Luo, Jian-Jun Chen, Qiang Lv, Jun Qin, Yi-Zhou Huang, Min-Hao Yu, Ming Zhong
In recent years, accumulating evidence has indicated that long non-coding RNAs (lncRNAs) are powerful factors influencing the progression of multiple malignancies. Although a relationship between the lncRNA NEAT1 (nuclear enriched abundant transcript 1) and colorectal cancer has previously been reported, the functional mechanism underlying the involvement of NEAT1 in colorectal cancer remains unknown. In this study, we report that NEAT1 expression is up-regulated in colorectal cancer tissues, which correlates with advanced clinical features, poor overall survival and disease free survival...
October 9, 2018: Cancer Letters
Zahra Heydari, Mahdi Rahaie, Ali Mohammad Alizadeh, Shahram Agah, Solmaz Khalighfard, Sahar Bahmani
A wide range of sources supports that the link between diet and colorectal cancer may be due to an imbalance of the intestinal microflora. In this case, it seems that the probiotics may have a possible molecular mechanism via microRNAs (miRNAs). The present study is aimed to evaluate the effects of Lactobacillus acidophilus and Bifidobacterium bifidum probiotics on the expression of miRNAs 135b, 26b, 18a, and 155 and their target genes, including APC, PTEN, KRAS, and PU.1 in mouse azoxymethane (AOM)-induced colon cancer...
October 11, 2018: Probiotics and Antimicrobial Proteins
Xiangxiang Liu, Xiaoxiang Chen, Kaixuan Zeng, Mu Xu, Bangshun He, Yuqin Pan, Huiling Sun, Bei Pan, Xueni Xu, Tao Xu, Xiuxiu Hu, Shukui Wang
As one of the most common cancers worldwide, colorectal cancer (CRC) causes a large number of mortality annually. Aberrant expression of microRNAs (miRNAs) is significantly associated with the initiation and development of CRC. Further investigations regarding the regulatory mechanism of miRNAs is warranted. In this study, we discovered that miR-486-5p was remarkably downregulated in CRC, which partially results from higher DNA methylation in the promoter region detected by using methylation-specific PCR, bisulfite sequencing PCR, and DNA demethylation treatment...
October 10, 2018: Cell Death & Disease
Yuanlin Xu, Xihong Zhang, Xiufeng Hu, Wenping Zhou, Peipei Zhang, Jiuyang Zhang, Shujun Yang, Yanyan Liu
BACKGROUND: For the study, we determine the potential biomarkers and uncover the regulatory mechanisms of lncRNA MALAT1 / miR-145 / SOX9 axis on the abilities of cell growth and cell metastasis of colorectal cancer. METHODS: Previously published dataset GSE18105 from GEO database was used for microarray analysis to identify differential-expressed lncRNAs and mRNAs. The miRNA which had targeted relationships with both lncRNA and mRNA was predicted using miRCode and Targetscan...
October 3, 2018: Molecular Medicine
Omar Youssef, Amal Fawzy, Reham Elshimy, Alhadi Almangush, Ahmed M Mahmoud, Iman Loay
BACKGROUND: Colorectal carcinoma development progresses through a sequence of normal mucosa-polyp-carcinoma. Early detection of premalignancy is crucial for improved outcomes. We evaluated the diagnostic performance of plasma miRNA-221 and its feasibility in discriminating premalignant from malignant neoplasms and correlating it with immunohistochemical p53 expression. METHODS: A total of 109 plasma samples were collected (76 carcinoma, 14 premalignant, and 19 controls)...
September 1, 2018: Clinical Laboratory
Binbin Xie, Rongjie Zhao, Bingjun Bai, Yuhui Wu, Yuzi Xu, Si Lu, Yong Fang, Zhanggui Wang, Ewetse Paul Maswikiti, Xiaoyun Zhou, Hongming Pan, Weidong Han
Colorectal cancer (CRC) is one of the most common malignant tumors and its development involves multi‑gene driven processes that affect the digestive system. The objective of this study was to identify tumorigenesis‑associated gene signatures using microarray expression profiling data. The gene expression profiling of GSE39582, a dataset containing 566 colon cancer samples and 19 non‑tumoral colorectal mucosae was downloaded from Gene Expression Omnibus (GEO) database. A total of 439 differentially expressed genes (DEGs) were extracted by GEO2R...
September 21, 2018: Oncology Reports
Jianyu Fan, Wenjing Zhang, Yanting Wu, Ping Wan, Qiang Guo, Yu Zhang
MicroRNA (miRNA/miR)-124 is a miRNA, which exerts tumor suppressive effects but is frequently absent in tumors. Although it has been validated to target oncogenic genes such as signal transducer and activator of transcription 3, forkhead box Q1, and Slug, the mechanistic link between miR‑124 and potential target genes that contribute to tumor progression, is yet to be investigated. IQ motif containing GTPase activating protein 1 (IQGAP1) is a scaffold protein that participates in protein‑protein interactions and integrating diverse signaling pathways...
September 27, 2018: Molecular Medicine Reports
Jun Hu, Xin Yue, Jianzhong Liu, Dalu Kong
The aim of the present study was to identify potential biomarkers associated with colorectal cancer (CRC). The GSE32323 and GSE53592 mRNA and microRNA (miRNA) expression profiles were selected from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) in CRC tissue samples compared with surrounding control tissue samples (DEGs‑CC), and DEGs in cells treated with 5‑aza‑2'‑deoxycitidine compared with untreated cells (DEGs‑TC) were identified with the Limma package...
September 24, 2018: Molecular Medicine Reports
Yili Tao, Shoubin Zhan, Yanbo Wang, Geyu Zhou, Hongwei Liang, Xi Chen, Hong Shen
Colorectal cancer (CRC) is among the most frequently occurring cancers worldwide. Baicalin is isolated from the roots of Scutellaria baicalensis and is its dominant flavonoid. Anticancer activity of baicalin has been evaluated in different types of cancers, especially in CRC. However, the molecular mechanisms underlying the contribution of baicalin to the treatment of CRC are still unknown. Here, we confirmed that baicalin can effectively induce and enhance apoptosis in HT-29 cells in a dose-dependent manner and suppress tumour growth in xenografted nude mice...
September 27, 2018: Scientific Reports
Grzegorz Hibner, Małgorzata Kimsa-Furdzik, Tomasz Francuz
Colorectal cancer (CRC) is currently the third and the second most common cancer in men and in women, respectively. Every year, more than one million new CRC cases and more than half a million deaths are reported worldwide. The majority of new cases occur in developed countries. Current screening methods have significant limitations. Therefore, a lot of scientific effort is put into the development of new diagnostic biomarkers of CRC. Currently used prognostic markers are also limited in assessing the effectiveness of CRC therapy...
September 27, 2018: International Journal of Molecular Sciences
Xianfeng Qu, Li Yang, Qingqing Shi, Xiaofei Wang, Dongguo Wang, Guiyang Wu
Lidocaine is a conventional local anesthetic which is shown antiproliferative of colorectal cancer (CRC) in patients. MicroRNAs (miRNAs) have been consistently demonstrated to be involved in CRC, and miR-520a-3p could suppress CRC migration, promote apoptosis by targeting epidermal growth factor receptor (EGFR). However, the mechanism by which lidocaine regulated CRC proliferation and apoptosis remains unknown. In this study, quantitative RT-PCR were used to measure miR-520a-3p and EGFR expression levels, and western blotting assays ware performed to measure EGFR expression in CRC cells...
September 13, 2018: Pathology, Research and Practice
Fiona C Malcomson, Naomi D Willis, Iain McCallum, Long Xie, Seamus Kelly, David Michael Bradburn, Nigel J Belshaw, Ian T Johnson, John C Mathers
Bowel cancer risk is strongly influenced by lifestyle factors including diet and physical activity. Several studies have investigated the effects of adherence to the World Cancer Research Fund (WCRF)/American Institute for Cancer Research (AICR) cancer prevention recommendations on outcomes such as all-cause and cancer-specific mortality, but the relationships with molecular mechanisms that underlie the effects on bowel cancer risk are unknown. This study aimed to investigate the relationships between adherence to the WCRF/AICR cancer prevention recommendations and wingless/integrated (WNT)-pathway-related markers of bowel cancer risk, including the expression of WNT pathway genes and regulatory microRNA (miRNA), secreted frizzled-related protein 1 (SFRP1) methylation and colonic crypt proliferative state in colorectal mucosal biopsies...
September 26, 2018: British Journal of Nutrition
Jun Zhao, Jingjie Cao, Lurong Zhou, Yunyi Du, Xiaoling Zhang, Bo Yang, Yangjun Gao, Yu Wang, Ning Ma, Wei Yang
Colorectal cancer (CRC) is the most common malignant tumor type and has become resistant to 5-fluorouracil (5-FU) in recent decades, which is one of the most popular therapies. Recently, microRNA (miRNA or miR) has been investigated as a potential therapeutic strategy for CRC. However, there has been little investigation of the underlying mechanism of the association between expression of miRNA and chemosensitivity. The present study aimed to investigate the effect of miR-1260b inhibitor on CRC cells, and their chemosensitivity to 5-FU, by treating them with the miR-1260b inhibitor...
October 2018: Oncology Letters
Xiaoxiang Chen, Kaixuan Zeng, Mu Xu, Xiuxiu Hu, Xiangxiang Liu, Tao Xu, Bangshun He, Yuqin Pan, Huiling Sun, Shukui Wang
Increasing long non-coding RNAs (lncRNAs) have been reported to play key roles in the development and progression of various malignancies. ZNFX1 antisense RNA1 (ZFAS1) has been reported to be aberrant expression and suggested as a tumor suppressor or oncogene in many cancers. However, the biological role and underlying molecular mechanism of ZFAS1, especially the miRNA sponge role of which in CRC remain largely unknown. We found that ZFAS1 expression was higher in CRC tissues, where it was associated with poor overall survival (OS), we also showed that ZFAS1 upregulation was induced by nuclear transcription factor SP1...
September 24, 2018: Cell Death & Disease
Suo-Yu Zhu, Qun-Ying Wu, Chen-Xia Zhang, Qiong Wang, Jing Ling, Xian-Ting Huang, Xia Sun, Ming Yuan, Dan Wu, Hua-Fang Yin
BACKGROUND: NK cells are presented in tumor microenvironments and acts as an essential defense line against multiple malignancies. Recently, miRNAs are reported to involve in the development of natural killer (NK) cells via negatively regulating gene expression. Here, we aim to explore the function and mechanism underlying how miR-20a modulated the killing effect of NK cells to cervical cancer cells. METHODS: Abundances of miR-20a and runt-related transcription factor 1 (RUNX1) in NK cells from cervical cancer patients and healthy donors were detected by qRT-PCR and western blot...
October 20, 2018: Biochemical and Biophysical Research Communications
Xiaolin Hou, Xuelai He, Kang Wang, Nengyi Hou, Junwen Fu, Guiqing Jia, Xiaofei Zuo, Haibo Xiong, Minghui Pang
BACKGROUND/AIMS: Colorectal cancer (CRC) is one of leading cancers in both incidence and mortality rate. The 5-year survival rate varies considerably depending on the pathological stage of the tumor. Although prominent progress has been made through screening for survival-associated factors from a certain type of genetic or epigenetic modifications, few attempts have been made to apply a network-based approach in prognostic factor identification, which could prove valuable for a complex, multi-faceted disease such as CRC...
2018: Cellular Physiology and Biochemistry
Lei Ding, Zhenwei Lan, Xianhui Xiong, Hongshun Ao, Yingting Feng, Huan Gu, Min Yu, Qinghua Cui
Colorectal cancer (CRC) is responsible for one of the major cancer incidence and mortality worldwide. It is well known that MicroRNAs (miRNAs) play vital roles in maintaining the cell development and other physiological processes, as well as, the aberrant expression of numerous miRNAs involved in CRC progression. MiRNAs are a class of small, endogenous, non-coding, single-stranded RNAs that bind to the 3'-untranslated region (3'-UTR) complementary sequences of their target mRNA, resulting in mRNA degradation or inhibition of its translation as a post-transcriptional regulators...
September 17, 2018: International Journal of Molecular Sciences
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