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https://www.readbyqxmd.com/read/30326263/new-perspectives-on-mega-plasmid-sequence-poh1-in-bacillus-thuringiensis-atcc-10792-harbouring-antimicrobial-insecticidal-and-antibiotic-resistance-genes
#1
Ramachandran Chelliah, Byung-Jae Park, Shuai Wei, Joong-Hyun Park, Youn-Seo Park, Se-Hun Kim, Yong-Guo Jin, Deog-Hwan Oh
Bacillus thuringiensis promotes the growth of numerous economically important crops. The present study presents the complete genome sequence for a mega plasmid present in the type strain of B. thuringiensis ATCC 10792, a typical spore-forming Gram-positive bacterium with insecticidal activity, and investigates its genetic characteristics. The genome was sequenced and assembled de novo using Pac-Bio sequencers and the Hierarchical Genome Assembly Process, respectively. Further genome annotation was performed, and a total of 489 proteins and a novel mega-plasmid (poh1) with 584,623 bps were identified...
October 13, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/30325291/addressing-intimate-partner-violence-to-improve-women-s-preconception-health
#2
Isabel A Morgan, Cheryl L Robbins, Kathleen C Basile
Exposure to violence can harm women's overall health and well-being. Data suggest that one in three women in the United States experience some form of violence by an intimate partner in their lifetime. In this commentary, we describe the implications of intimate partner violence (IPV) on women's health, specifically for women of reproductive age. We use a life-course perspective to describe the compounded impact of IPV on preconception health. Preconception health generally refers to the overall health and well-being of women (and men) before pregnancy...
October 2018: Journal of Women's Health
https://www.readbyqxmd.com/read/30323395/reflections-on-the-pharmacist-patient-covenant
#3
Amy M Haddad
The first tenet of the Code of Ethics for Pharmacists of the American Pharmacists Association describes the relationship between the pharmacist and the patient as a covenant. The central argument of this commentary is that the symbolic language of covenant, the metaphor used to describe the pharmacist-patient relationship, should be formally acknowledged as an ideal theory or concept. However, before committing to this ideal, there should be resonance with the realities of pharmacy practice. This commentary argues that the intimate nature of a covenantal relationship is not a good fit with most contexts in which pharmacists practice...
September 2018: American Journal of Pharmaceutical Education
https://www.readbyqxmd.com/read/30322854/inactivation-of-the-monofunctional-peptidoglycan-glycosyltransferase-sgtb-allows-staphylococcus-aureus-to-survive-in-the-absence-of-lipoteichoic-acid
#4
Eleni Karinou, Christopher F Schuster, Manuel Pazos, Waldemar Vollmer, Angelika Gründling
The cell wall of Staphylococcus aureus is composed of peptidoglycan and the anionic polymers lipoteichoic acid (LTA) and wall teichoic acid. LTA is required for growth and normal cell morphology in S. aureus Strains lacking LTA are usually only viable when grown under osmotically stabilizing conditions or after the acquisition of compensatory mutations. LTA negative suppressor strains with inactivating mutations in gdpP , resulting in an increase in intracellular c-di-AMP levels, have been described previously...
October 15, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/30322509/-life-then-life-interrupted-life-reclaimed-the-fluctuation-of-agency-in-teenagers-and-young-adults-with-cancer
#5
Jane Davies, Daniel Kelly, Ben Hannigan
PURPOSE: This paper presents findings from an in-depth study exploring agency in decision making when young people experience cancer. METHODS: Exploratory case studies were utilised to examine all aspects of decision making, beginning with the onset of symptoms followed by diagnosis and treatment. Interviews, observations and documentary analysis were used to generate data. Multiple sources were selected to generate further insights and these included family, partners, friends and healthcare professionals who were close to the young people during their experience...
October 2018: European Journal of Oncology Nursing: the Official Journal of European Oncology Nursing Society
https://www.readbyqxmd.com/read/30322508/an-ethnographic-inquiry-into-the-psychosocial-care-for-oncology-patients-in-the-community-healing-from-the-4es
#6
Engle Angela Chan, Shirley Ching, Michael Li
PURPOSE: The aim of this study was to explore how the provision of community care in a cancer support center can help cancer patients and survivors cope with the demands of cancer and its treatment. METHODS: A focused ethnographic approach was adopted. Data were collected through participant observations and focus groups with various stakeholders. The data from the focus groups were transcribed verbatim and analyzed using constant comparative analysis. RESULTS: Four main themes emerged: 1...
October 2018: European Journal of Oncology Nursing: the Official Journal of European Oncology Nursing Society
https://www.readbyqxmd.com/read/30321512/activation-of-toll-like-receptor-3-promotes-pathological-corneal-neovascularization-by-enhancement-of-sdf-1-mediated-endothelial-progenitor-cell-recruitment
#7
Ruijuan Zhao, Jing Zhang, Yan Wang, Jiayi Jin, Hongyan Zhou, Jianping Chen, Shao Bo Su
Toll-like receptors (TLRs) play an important role in inflammatory and immunological responses, which are intimately related to neovascularization. However, the precise mode of action of TLR3 in neovascularization still remains ambiguous. In this study, we sought to investigate the role of TLR3 in pathological corneal neovascularization (CNV) using a mouse model of alkali-induced CNV. CNV was attenuated in TLR3-deficient mice, and the absence of TLR3 led to decreased production of stromal cell-derived factor 1 (SDF-1), a well-characterized cytokine that regulates the recruitment of endothelial progenitor cells (EPCs) to the sites of neo-angiogenic niches in the injured tissues...
October 12, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/30321344/a-novel-subfamily-of-bacterial-aat-fold-basic-amino-acid-decarboxylases-and-functional-characterization-of-its-first-representative-pseudomonas-aeruginosa-ldca
#8
Diego Carriel, Pierre Simon Garcia, Florence Castelli, Patricia Lamourette, François Fenaille, Céline Brochier-Armanet, Sylvie Elsen, Irina Gutsche
Polyamines are small amino-acid derived polycations capable of binding negatively charged macromolecules. Bacterial polyamines are structurally and functionally diverse, and are mainly produced biosynthetically by PLP-dependent amino acid decarboxylases referred to as LAOdcs (Lysine-Arginine-Ornithine decarboxylases). In a phylogenetically limited group of bacteria, LAOdcs are also induced in response to acid stress. Here, we performed an exhaustive phylogenetic analysis of the AAT-fold LAOdcs which showcased the ancient nature of their short forms in Cyanobacteria and Firmicutes, and emergence of distinct subfamilies of long LAOdcs in Proteobacteria...
October 13, 2018: Genome Biology and Evolution
https://www.readbyqxmd.com/read/30321136/women-s-autonomy-and-intimate-partner-violence-in-ghana
#9
Eric Y Tenkorang
CONTEXT: Previous studies have established women's autonomy as an important determinant of several demographic outcomes in Sub-Saharan Africa, yet very few have considered intimate partner violence as one of these outcomes. METHODS: Data collected in 2017 from 2,289 women residing in 40 communities in Ghana were used to examine associations between three types of autonomy-economic decision making, family planning decision making and sexual autonomy-and women's experiences with physical, sexual, emotional and economic violence...
June 1, 2018: International Perspectives on Sexual and Reproductive Health
https://www.readbyqxmd.com/read/30320560/timing-of-clopidogrel-loading-dose-on-peripheral-blood-endothelial-progenitor-cells-sdf-1%C3%AE-and-neointimal-hyperplasia-in-carotid-stenting
#10
Giuseppe Di Stolfo
BACKGROUND: Carotid stenting stimulates intimal proliferation through platelet and stem cell activation. OBJECTIVE: The aim of this study is to evaluate whether the administration before or after carotid stenting of clopidogrel loading dose may play a role on circulating endothelial progenitor cells, stromal cell-derived factor-1α (SDF-1α) and neointimal hyperplasia. METHODS: We recruited 13 patients (aged 74.52±7.23) with indication of carotid revascularization and in therapy with salicylic acid and statin...
September 29, 2019: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/30319489/intimate-partner-violence-and-its-escalation-into-femicide-frailty-thy-name-is-violence-against-women
#11
Georgia Zara, Sarah Gino
Violence against women is a disabler of dignity, liberty, and rights of the person, with murder being its extreme form for silencing the individual. Despite psycho-criminological research providing evidence that violence can happen across cultures, sexes, and societies, other findings show that some forms of violence i.e. Intimate Partner Violence (IPV), which involves more frequently women as victims, is not rare in contemporary society. The aim of this study is to analyze the violence against women, and how it escalates up to the point in which it aggravates into femicide...
2018: Frontiers in Psychology
https://www.readbyqxmd.com/read/30319479/making-worlds-in-a-waking-dream-where-bion-intersects-friston-on-the-shaping-and-breaking-of-psychic-reality
#12
Matthew John Mellor
With the publication of Wilfred Bion's text 'Learning from Experience,' psychoanalysis was afforded a new schema for understanding the processes and implications involved in an infant's contact with their caregivers. As a result, our conception of some of the most fundamental phenomena of psychic life was significantly enriched. By proposing his theory of alpha-functioning, Bion mapped out how meaningful connexions to the internal and external worlds become established in the mind. In contrast, and through working clinically with psychotic patients, Bion revealed how these ties can catastrophically come undone...
2018: Frontiers in Psychology
https://www.readbyqxmd.com/read/30319438/biomechanical-material-characterization-of-stanford-type-b-dissected-porcine-aortas
#13
Aashish Ahuja, Jillian N Noblet, Tony Trudnowski, Bhavesh Patel, Joshua F Krieger, Sean Chambers, Ghassan S Kassab
Aortic dissection (AD) involves tearing of the medial layer, creating a blood-filled channel called false lumen (FL). To treat dissections, clinicians are using endovascular therapy using stent grafts to seal the FL. This procedure has been successful in reducing mortality but has failed in completely re-attaching the torn intimal layer. The use of computational analysis can predict the radial forces needed to devise stents that can treat ADs. To quantify the hyperelastic material behavior for therapy development, we harvested FL wall, true lumen (TL) wall, and intimal flap from the middle and distal part of five dissected aortas...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/30319414/28-day-oral-chronic-toxicity-study-of-arctigenin-in-rats
#14
Yu-Jun Tan, Yu-Shan Ren, Lei Gao, Lan-Fang Li, Li-Juan Cui, Bin Li, Xin Li, Jian Yang, Ming-Zhi Wang, Yuan-Yuan Lv, Xiao-Li Xu, Jing-Chun Yao, Zhong Liu, Gui-Min Zhang, Jie Li
Arctium lappa (burdock) is the most popular daily edible vegetable in China and Japan because of its general health tonic effects. Previous studies focused on the beneficial role of Arctigenin but neglected its potential side-effects and toxicities. In the present study, the sub-chronic toxicity profile of Arctigenin following 28 days of consecutive exposure was investigated in rats. The results showed that during the drug exposure period, Arctigenin -12 mg/kg administration resulted in focal necrosis and lymphocytes infiltration of heart ventricular septal muscle cells...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/30318636/ultrastable-graphene-encapsulated-3-nm-nanoparticles-by-in-situ-chemical-vapor-deposition
#15
Dong Sung Choi, Chanhoon Kim, Joonwon Lim, Su-Ho Cho, Gil Yong Lee, Ho Jin Lee, Jang Wook Choi, Heeyeon Kim, Il-Doo Kim, Sang Ouk Kim
Nanoscale materials offer enormous opportunities for catalysis, sensing, energy storage, and so on, along with their superior surface activity and extremely large surface area. Unfortunately, their strong reactivity causes severe degradation and oxidation even under ambient conditions and thereby deteriorates long-term usability. Here superlative stable graphene-encapsulated nanoparticles with a narrow diameter distribution prepared via in situ chemical vapor deposition (CVD) are presented. The judiciously designed CVD protocol generates 3 nm size metal and ceramic nanoparticles intimately encapsulated by few-layer graphene shells...
October 15, 2018: Advanced Materials
https://www.readbyqxmd.com/read/30317399/shear-bond-strength-and-interface-analysis-between-a-resin-composite-and-a-recent-high-viscous-glass-ionomer-cement-bonded-with-various-adhesive-systems
#16
Philippe Francois, Elsa Vennat, Stéphane Le Goff, Nathalie Ruscassier, Jean-Pierre Attal, Elisabeth Dursun
OBJECTIVE: This study investigated the shear bond strength (SBS) and interface between a resin composite and a new high-viscous glass ionomer cement (HV-GIC), a HV-GIC, a resin-modified glass ionomer cement (RM-GIC), a bulk-fill flowable composite, and a regular flowable composite bonded with various adhesive systems. METHODS AND MATERIALS: A resin composite (Filtek Z350) was bonded to a new HV-GIC (EQUIA Forte Fil) using various adhesive systems, including a universal adhesive in self-etch and etch-and-rinse mode (Scotchbond Universal), a two-step etch-and-rinse adhesive (Scotchbond 1-XT), a one-step self-etch adhesive (Optibond All-in-one) tested also after silane application (Monobond Plus), and a coating material (EQUIA Forte Coat)...
October 13, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/30317242/homocysteine-level-in-children-with-classic-congenital-adrenal-hyperplasia-relationship-to-carotid-intimal-wall-thickness-and-left-ventricular-function
#17
Kotb Abbass Metwalley, Hekma Saad Farghaly, Abdelrahman Abdelhamid
BACKGROUND/AIMS: Homocysteine is an important and independent risk factor for atherosclerotic diseases. The aim of this study was to evaluate serum levels of homocysteine in children with congenital adrenal hyperplasia (CAH) and their relation to carotid artery intima-media thickness (CA-IMT) and left ventricular (LV) function. METHODS: This study included 36 children with classic CAH and 36 healthy children. All underwent anthropometric evaluation. Measurement of serum levels of total homocysteine was carried out...
October 12, 2018: Hormone Research in Pædiatrics
https://www.readbyqxmd.com/read/30317114/optically-robust-highly-permeable-and-elastic-protein-films-that-support-dual-cornea-cell-types
#18
Behnaz Aghaei-Ghareh-Bolagh, Juan Guan, Yiwei Wang, Adam D Martin, Rebecca Dawson, Suzanne M Mithieux, Anthony S Weiss
Damaged corneas can lead to blindness. Due to the worldwide shortage of donor corneas there is a tremendous unmet demand for a robust corneal replacement that supports growth of the major corneal cell types. Commercial artificial corneas comprise plastic polymers that do not adequately support diverse cell growth. We present a new class of protein elastomer-dominated synthetic corneas with attractive performance that intimately couple biologically active tropoelastin to mechanically robust and durable protein silk...
October 8, 2018: Biomaterials
https://www.readbyqxmd.com/read/30317110/diversity-and-characterization-of-spoilage-associated-psychrotrophs-in-food-in-cold-chain
#19
Yuxiang Zhang, Jianping Wei, Yahong Yuan, Tianli Yue
In this work, psychrotrophs known to cause food spoilage were isolated from commercial food products. Further, temperature sensitivities and volatile organic compounds of the representative strains were characterized to evaluate the population heterogeneity. A total of 490 isolates belonging to 38 genera of 20 families were identified from 30 psychrotroph-positive samples, among which Gram-negative bacteria occurred frequently. The genus Pseudomonas exhibited a clear predominance, especially Pseudomonas fragi, followed by Psychrobacter, Brochothrix, Serratia, and Stenotrophomonas, with the dominant bacteria varying with origin...
October 2, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/30317081/sn-doped-hematite-modified-by-camn-2-o-4-nanowire-with-high-donor-density-and-enhanced-conductivity-for-photocatalytic-water-oxidation
#20
Aijuan Liu, Chunyong Zhang, Yongcheng Zhu, Kezhen Li, Jie Huang, Yukou Du, Ping Yang
Herein, we report a novel nanocomposite consisting of n-type Sn-doped hematite and p-type CaMn2 O4 nanowire (CaMn2 O4 /α-Fe2 O3 ). The nanocomposite was characterized by X-ray diffraction (XRD), high resolution transmission electron microscopy (HRTEM), ultraviolet-visible absorption spectroscopy, X-ray photoelectron spectroscopy (XPS), which showed that nanospindle-like Sn-doped hematite and CaMn2 O4 nanowire contact intimately in the nanocomposite, resulting in efficient charge transfer and separation. Photoelectrochemical results reveal that the nanocomposite possesses higher donor density, enhanced conductivity and lower overpotential for dioxygen evolution...
October 1, 2018: Journal of Colloid and Interface Science
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