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https://www.readbyqxmd.com/read/30529045/-epstein-barr-virus-primary-infection-in-healthy-children
#1
Mónica García-Peris, M Isabel Jiménez Candel, Yolanda Mañes Jiménez, Macarena Pariente Martí, Damiana González Granda, Fernando Calvo Rigual
INTRODUCTION: The aim of this study is to assess epidemiological, clinical and laboratory characteristics of primary infection by Epstein-Barr virus (EBV) in children without previous diagnosis of any immune disease and its relationship with clinical presentation. PATIENTS AND METHODS: A retrospective study was conducted on all children from 0 to 15 years with IgM against viral capsid of EBV positive or indeterminate during a 22 month period. Epidemiological, clinical and laboratory data were analysed and compared between typical (mononucleosis syndrome) and non-typical clinical symptoms...
October 24, 2018: Anales de Pediatría: Publicación Oficial de la Asociación Española de Pediatría (A.E.P.)
https://www.readbyqxmd.com/read/30472113/behavioral-characterization-of-dat-ko-rats-and-evidence-of-asocial-like-phenotypes-in-dat-het-rats-the-potential-involvement-of-norepinephrine-system
#2
Annalisa Adinolfi, Silvia Zelli, Damiana Leo, Cristiana Carbone, Liudmila Mus, Placido Illiano, Enrico Alleva, Raul R Gainetdinov, Walter Adriani
Dopamine (DA) is a key neurotransmitter of the central nervous system, whose availability is regulated by the dopamine transporter (DAT). Deletion of DAT gene leading to hyperdopaminergia was previously performed on mouse models. This enabled recapitulation of the core symptoms of Attention-Deficit / Hyper-activity Disorder (ADHD), which include hyperactivity, inattention and cognitive impairment. We used recently developed DAT knockout (DAT-KO) rats to carry out further behavioral profiling on this novel model of hyperdopaminergia...
November 22, 2018: Behavioural Brain Research
https://www.readbyqxmd.com/read/30449354/sporothrix-brasiliensis-induces-a-more-severe-disease-associated-with-sustained-th17-and-regulatory-t-cells-responses-than-sporothrix-schenckii-sensu-stricto-in-mice
#3
Alexander Batista-Duharte, Damiana Téllez-Martínez, Cleverton Roberto de Andrade, Deivys Leandro Portuondo, Juliana Aparecida Jellmayer, Marisa Campos Polesi, Iracilda Zeppone Carlos
Little is known about the differences in the CD4+ T-cell response induced by Sporothrix schenckii and Sporothrix brasiliensis, the most virulent species that cause sporotrichosis. Here, the helper (Th) and regulatory T cells (Tregs) responses were evaluated comparatively in a murine model of sporotrichosis on days 7, 21 and 35 after subcutaneous infection with either S. schenckii or S. brasiliensis conidia. The fungal load was measured at the site of infection, as well as in the liver and spleen. The Th1/Th17/Tregs responses were analyzed in the spleen, while the level of IL-2, IL-4, IL-6, TNF-alpha, IFN-ɣ, IL-17A and IL-10 cytokines were measured at the local site of infection on 24 h postinfections and in sera on the indicated days...
December 2018: Fungal Biology
https://www.readbyqxmd.com/read/30441762/metabolomic-signature-in-sera-of-multiple-sclerosis-patients-during-pregnancy
#4
Claudia Rossi, Ilaria Cicalini, Mirco Zucchelli, Maria di Ioia, Marco Onofrj, Luca Federici, Piero Del Boccio, Damiana Pieragostino
Multiple sclerosis (MuS) is an autoimmune disease of the central nervous system characterized by neuroinflammation, neurodegeneration, and degradation of the myelin sheath. Epidemiological studies have shown that the female gender is more susceptible than the male gender to MuS development, with a female-to-male ratio of 2:1. Despite this high onset, women have a better prognosis than men, and the frequency of the relapsing phase decreases during pregnancy, while it increases soon after birth. Therefore, it is interesting to investigate hormonal fluctuations during pregnancy and whether they correlate with metabolic signatures...
November 14, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30427528/theileria-sp-in-water-buffaloes-from-maranh%C3%A3-o-state-northeastern-brazil
#5
Hugo Luca Abate, Nelson Jessé Rodrigues Dos Santos, Danilo Rodrigues Barros Brito, Jessica Damiana Marinho Valente, Thállitha Samih Wischral Jayme Vieira, João Luis Garcia, Rafael Felipe da Costa Vieira, Odilon Vidotto
Anaplasma marginale and piroplasm species are widespread among Brazilian cattle herds. Both of these tick-borne pathogens hamper livestock production and cause a significant economic impact. Although buffaloes have demonstrated a high level of adaptability, data on tick-borne pathogens are scarcely reported in Brazil. Thus, the aim of this study was to screen water buffaloes from the state of Maranhão for piroplasm and A. marginale occurrence using PCR assays. All samples were negative for A. marginale. One of the 287 (0...
November 8, 2018: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/30365819/factors-that-influence-the-redox-state-in-children-an-exploratory-study
#6
Sandra Trindade Low, Ana Paula Costa Rodrigues Ferraz, Regiane Maio, Fabiane Valentini Francisqueti, Damiana Tortolero Pierine, Weidylla Natália Silva Borges, Ariel Dandara de Carvalho, Gedalva Pereira de Lima, Ana Lúcia A Ferreira, Klinsmann Carolo Dos Santos, Camila Renata Corrêa
OBJECTIVE: The aim of the present study was to investigate the association of the redox state via malondialdehyde (MDA) as a lipid peroxidation biomarker and hydrophilic antioxidant capacity (HAC) with dietary, anthropometric, demographic, socio-economic and clinical variables as well as the serum concentrations of vitamins in children aged 20-36 months. This cross-sectional study was conducted from May 2013 to May 2014 and included a total of 100 children. METHODS: The variables studied included anthropometric measurements, dietary intake by the Food Frequency Questionnaire (FFQ), socio-demographic features, clinical attributes, serum redox status, and serum vitamin concentrations...
October 18, 2018: Clinics
https://www.readbyqxmd.com/read/30323131/novel-translational-rat-models-of-dopamine-transporter-deficiency
#7
Damiana Leo, Ilya Sukhanov, Raul R Gainetdinov
No abstract text is available yet for this article.
December 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/30281891/a-metabolic-control-analysis-approach-to-introduce-the-study-of-systems-in-biochemistry-the-glycolytic-pathway-in-the-red-blood-cell
#8
Carla R Angelani, Pablo Carabias, Karen M Cruz, José M Delfino, Marilina de Sautu, María V Espelt, Mariela S Ferreira-Gomes, Gabriela E Gómez, Irene C Mangialavori, Malena Manzi, María F Pignataro, Nicolás A Saffioti, Damiana M Salvatierra Fréchou, Javier Santos, Pablo J Schwarzbaum
Metabolic control analysis (MCA) is a promising approach in biochemistry aimed at understanding processes in a quantitative fashion. Here the contribution of enzymes and transporters to the control of a given pathway flux and metabolite concentrations is determined and expressed quantitatively by means of numerical coefficients. Metabolic flux can be influenced by a wide variety of modulators acting on one or more metabolic steps along the pathway. We describe a laboratory exercise to study metabolic regulation of human erythrocytes (RBCs)...
September 2018: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/30261551/lymphomagenic-properties-of-a-hiv-p17-variant-derived-from-a-splenic-marginal-zone-lymphoma-occurred-in-a-hiv-infected-patient
#9
Francesca Caccuri, Elena Muraro, Annunziata Gloghini, Ombretta Turriziani, Mara Riminucci, Cinzia Giagulli, Katy Mastorci, Damiana Antonia Fae', Simona Fiorentini, Antonino Carbone, Arnaldo Caruso, Riccardo Dolcetti
Despite antiretroviral therapy, HIV+ individuals still have increased risk to develop lymphomas, including marginal zone lymphomas, suggesting that factors other than HIV-related immunosuppression are probably acting as lymphomagenic factors in the HIV setting. The possible pathogenic involvement of HIV p17 protein variants was investigated in a particularly informative case of HIV-related splenic marginal zone lymphoma, who was negative for oncogenic virus infections, thus allowing us to assess the possible direct contribution of these HIV-encoded proteins to lymphomagenesis...
September 27, 2018: Hematological Oncology
https://www.readbyqxmd.com/read/30127123/evidence-for-accuracy-of-pain-assessment-and-painkillers-utilization-in-neuropsychiatric-symptoms-of-dementia-in-calabria-region-italy
#10
Damiana Scuteri, Maria Roberta Garreffa, Stefania Esposito, Giacinto Bagetta, Maria Diana Naturale, Maria Tiziana Corasaniti
During the clinical course of dementia, beside cognitive impairment and memory loss, a very complex challenge is posed by the neuropsychiatric symptoms (NPSs). Accurate evaluation and treatment of pain impacts positively the agitation of demented patients aged ≥ 65 years. To gather information on the utilization of pain killers in demented patients a preliminary survey has been conducted in collaboration with the Calabrian Pharmacovigilance Territorial Service of the health district of Catanzaro (Italy). The study has taken into consideration the prescriptions of acetylcholinesterase inhibitors and memantine during the period ranging from July 2015 to June 2016 and the percentage of patients treated against pain with non steroidal antinflammatory drugs, opioids, and anticonvulsants have been monitored...
September 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/30114702/reproducibility-and-clinical-correlates-of-supine-diaphragmatic-motion-measured-by-m-mode-ultrasonography-in-healthy-volunteers
#11
Simone Scarlata, Damiana Mancini, Alice Laudisio, Augusto Benigni, Raffaele Antonelli Incalzi
BACKGROUND: Assessing diaphragm mobility is important to detect malfunctions or impending exhaustion and to evaluate the effects of many chest and abdominal conditions on respiratory mechanics. Amongst several imaging methods, ultrasonography represents the only noninvasive, nonionizing imaging technique widely available for the direct assessment of diaphragm excursion. OBJECTIVES: the aim of this study is to prospectively assess the supine diaphragmatic motion amplitude, intra- and inter-observer agreement, and anthropometric correlates of diaphragm motion variability, measured through M-mode ultrasonography, in a sample of healthy volunteers...
2018: Respiration; International Review of Thoracic Diseases
https://www.readbyqxmd.com/read/30051543/multilocus-sequence-typing-reveals-clonality-in-saprolegnia-parasitica-outbreaks
#12
Damiana Ravasi, Sophie De Respinis, Thomas Wahli
The molecular epidemiology of fish pathogen Saprolegnia parasitica is still largely unknown. We developed a multilocus sequence typing scheme based on seven housekeeping genes to characterize 77 S. parasitica strains isolated from different fish host species at different times and from different geographic areas in Switzerland between 2015 and 2017. Ten different diploid sequence types (DSTs) were identified. The majority (52%) of outbreaks in Switzerland seemed to be caused by one genotype, namely DST3, which was recovered from farm-raised and wild-caught fish in all the geographic areas and river basins included in the study...
July 27, 2018: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29977204/biochemical-and-functional-characterization-of-the-trace-amine-associated-receptor-1-taar1-agonist-ro5263397
#13
Stefano Espinoza, Damiana Leo, Tatyana D Sotnikova, Mohammed Shahid, Tiina M Kääriäinen, Raul R Gainetdinov
Trace amine-associated receptor 1 (TAAR1) is a G protein-coupled receptor, which signals through elevating intracellular cAMP levels, and expressed in most vertebrates, including rodents and humans. In recent years, several lines of evidence indicated the role of TAAR1 in the regulation of dopaminergic system and its importance in physiological processes such as locomotion, control of emotional states and cognition. In our study, we used RO5263397, a selective TAAR1 agonist, as a tool and characterized its pharmacology in vitro in HEK293 cells and its effects in vivo in tests assessing potential antidepressant and antipsychotic actions...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29943950/diagnosis-of-sudden-cardiac-death-due-to-early-myocardial-ischemia-an-ultrastructural-and-immunohistochemical-study
#14
Silvia Damiana Visonà, Donatella Benati, Maria Cristina Monti, Mirco Galiè, Luisa Andrello, Andrea Frontini, Antonio Osculati
The aim of this post-mortem ultrastructural and immunohistochemical study is to explore the characteristics of acute myocardial ischemia in the context of sudden death, using the combination of two different methods, both more insightful than ordinary histology. Transmission electron microscope and immunohistochemistry, in addition to the traditional histology, were applied to study human heart specimens collected during forensic autopsies. The whole series was sub-grouped into cases (n=17) and controls (n=10)...
April 3, 2018: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/29906556/antinociceptive-effect-of-inhalation-of-the-essential-oil-of-bergamot-in-mice
#15
Damiana Scuteri, Michele Crudo, Laura Rombolà, Chizuko Watanabe, Hirokazu Mizoguchi, Shinobu Sakurada, Tsukasa Sakurada, Rosaria Greco, Maria Tiziana Corasaniti, Luigi Antonio Morrone, Giacinto Bagetta
Bergamot essential oil (BEO) has proven wide evidence of pharmacological antinociceptive effectiveness both in nociceptive and in neuropathic pain models. The antinociceptive properties of BEO for inhalation have not been investigated. The purpose of this study is to evaluate the effects of the inhalation of BEO on formalin-induced nociceptive response in mice. Male ddY-strain mice (Japan SLC, Hamamatsu, Japan) of 23-25 g of weight at the time the experiments underwent the formalin test. Twenty μl of formalin (2% in saline) were administered into the plantar surface of the mice hindpaw and the time of licking/biting was observed and recorded at intervals of 5 min...
September 2018: Fitoterapia
https://www.readbyqxmd.com/read/29894962/efficacy-and-safety-of-immunological-adjuvants-where-is-the-cut-off
#16
REVIEW
Alexander Batista-Duharte, Damiana Téllez Martínez, Iracilda Zeppone Carlos
Research over the past several decades has provided insight into the mode of action of adjuvants. However, the main focus of attention has been the efficacy in the induction of protective immunogenicity, while less effort has been devoted to the study of toxicity mechanisms. Evidences suggest that several mechanisms that are responsible for the immunostimulating effects are, at the same time, responsible of the adverse effects. In this context, it is often very difficult to establish the boundaries between immunostimulation and immunotoxicity to reach the ideal balance of efficacy/safety...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29858951/abundance-and-diversity-of-anammox-bacteria-in-a-mainstream-municipal-wastewater-treatment-plant
#17
Ali Nejidat, Damiana Diaz-Reck, Nedal Massalha, Adi Arbiv, Anwar Dawas, Carlos Dosoretz, Isam Sabbah
Among the factors that obstruct the application of anammox-based technology for nitrogen removal from mainstream municipal wastewater is the water's high organic loads. We hypothesized that some anammox species can adapt and grow in mainstream wastewater in which a minimal temperature of 13-15 °C is maintained. Using the AMX368F and AMX820R PCR-primers, anammox bacteria were detected in influent wastewater (COD/N ratio > 13) and in the anaerobic, anoxic, and aerobic chambers of a full-scale municipal wastewater treatment plant, reaching 107 copies/g VSS of the16S rRNA gene...
August 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29843897/major-bleeding-with-old-and-novel-oral-anticoagulants-how-to-manage-it-focus-on-reversal-agents
#18
Stefania Angela Di Fusco, Fabiana Lucà, Manuela Benvenuto, Annamaria Iorio, Damiana Fiscella, Fabrizio D'Ascenzo, Andrea Madeo, Furio Colivicchi, Andrea Di Lenarda, Michele Massimo Gulizia
Even though vitamin K antagonists (VKAs) have been employed for >50 years, there is still some uncertainty about the best strategy to reverse anticoagulation due to VKAs in cases of major bleeding. Furthermore, there is also scarce evidence about the most appropriate way to treat serious bleeding associated with non-vitamin K antagonist oral anticoagulants. This review analyses the main advantages and disadvantages of the various forthcoming therapeutic options to restore a normal coagulation status in anticoagulated patients with ongoing serious bleeding...
October 1, 2018: International Journal of Cardiology
https://www.readbyqxmd.com/read/29799450/repeated-exposition-to-mercury-ii-chloride-enhances-susceptibility-to-s-schenckii-sensu-stricto-infection-in-mice
#19
Alexander Batista-Duharte, Damiana Téllez-Martínez, Juliana Aparecida Jellmayer, Deivys Leandro Portuondo Fuentes, Marisa Campos Polesi, Amanda Martins Baviera, Iracilda Zeppone Carlos
Sporotrichosis is a subcutaneous mycosis that has re-emerged in several tropical and subtropical regions over the last decades. Growing findings suggest that the interplay of host, pathogen, and environment has a determinant effect on the diversity, local distribution, and virulence of Sporothrix schenckii sensu lato , the etiologic agent. Among the environmental factors, we have studied the potential role of repeated exposures to mercury (Hg), a known immunotoxic xenobiotic that is widely used in gold mining regions where sporotrichosis outbreaks are frequently reported...
May 25, 2018: Journal of Fungi (Basel, Switzerland)
https://www.readbyqxmd.com/read/29785155/the-volume-of-the-cerebellum-in-the-second-semester-of-gestation
#20
Damiana Vulturar, Alexandru Fărcăşanu, Flaviu Turcu, Dan Boitor, Carmen Crivii
Background and aims: The cerebellum ("little brain"), the largest part of hind brain, lies in the posterior cranial fossa, beneath the occipital lobe and dorsal to the brainstem. It develops over a long period: it is one of the first structures in the brain to begin to differentiate, but one of the last to mature. The use of ultrasonography has significantly improved the evaluation of fetal growth and development and has permitted prenatal diagnosis of a variety of congenital malformations...
2018: Clujul Medical (1957)
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