Bruno A Cisterna, Kristen Skruber, Makenzie L Jane, Caleb I Camesi, Ivan D Nguyen, Tatiana M Liu, Peyton V Warp, Joseph B Black, Mitchell T Butler, James E Bear, Danielle E Mor, Tracy-Ann Read, Eric A Vitriol
In addition to its well-established role in actin assembly, profilin 1 (PFN1) has been shown to bind to tubulin and alter microtubule growth. However, whether PFN1's predominant control over microtubules in cells occurs through direct regulation of tubulin or indirectly through the polymerization of actin has yet to be determined. Here, we manipulated PFN1 expression, actin filament assembly, and actomyosin contractility and showed that reducing any of these parameters for extended periods of time caused an adaptive response in the microtubule cytoskeleton, with the effect being significantly more pronounced in neuronal processes...
July 1, 2024: Journal of Cell Biology