Melody R Lindsay, Timothy D'Angelo, Jacob H Munson-McGee, Alireza Saidi-Mehrabad, Molly Devlin, Julia McGonigle, Elizabeth Goodell, Melissa Herring, Laura C Lubelczyk, Corianna Mascena, Julia M Brown, Greg Gavelis, Jiarui Liu, D J Yousavich, Scott D Hamilton-Brehm, Brian P Hedlund, Susan Lang, Tina Treude, Nicole J Poulton, Ramunas Stepanauskas, Duane P Moser, David Emerson, Beth N Orcutt
Rates of microbial processes are fundamental to understanding the significance of microbial impacts on environmental chemical cycling. However, it is often difficult to quantify rates or to link processes to specific taxa or individual cells, especially in environments where there are few cultured representatives with known physiology. Here, we describe the use of the redox-enzyme-sensitive molecular probe RedoxSensor™ Green to measure rates of anaerobic electron transfer physiology (i.e., sulfate reduction and methanogenesis) in individual cells and link those measurements to genomic sequencing of the same single cells...
April 9, 2024: Proceedings of the National Academy of Sciences of the United States of America