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https://www.readbyqxmd.com/read/28445599/growth-engineering-of-synechococcus-elongatus-pcc-7942-for-mixotrophy-under-natural-light-conditions-for-improved-feedstock-production
#1
Aditya Sarnaik, Reena Pandit, Arvind Lali
Synechococcus elongatus PCC 7942 has been widely explored as cyanobacterial cell factory through genetic modifications for production of various value-added compounds. However, successful industrial scale-ups have not been reported for the system predominantly due to its obligate photoautotrophic metabolism and use of artificial light in photobioreactors. Hence, engineering the organism to perform mixotrophy under natural light could serve as an effective solution. Thus, we applied a genetically engineered strain of Synechococcus elongatus PCC 7942 expressing heterologous hexose transporter gene (galP) to perform mixotrophy under natural light in a temperature controlled environmental chamber (EC)...
April 26, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28440636/probing-and-comparing-the-photobromination-and-photoiodination-of-dissolved-organic-matter-by-using-ultrahigh-resolution-mass-spectrometry
#2
Zhineng Hao, Yongguang Yin, Dong Cao, Jing-Fu Liu
Photochemical halogenation of dissolved organic matter (DOM) may represent an important abiotic source of natural organobromine compounds (OBCs) and natural organoiodine compounds (OICs) within surface waters. Here we report the significantly enhanced formation of OBCs and OICs by photohalogenating DOM in freshwater and seawater, as well as the noticeable difference in distribution and composition pattern of newly formed OBCs and OICs. By using the negative ion electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry, numerous OBCs and OICs were identified during the photo halogenation processes in sunlit waters...
April 25, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28439687/colloid-characterization-and-in-situ-release-in-shallow-groundwater-under-different-hydrogeology-conditions
#3
Jingjing Zhou, Dan Liu, Wenjing Zhang, Xuequn Chen, Ying Huan, Xipeng Yu
Changes to groundwater hydrodynamics and chemistry can lead to colloid release that can have a major impact on the groundwater environment. To analyze the effects of colloid release caused by artificial groundwater recharge, field and laboratory tests on colloid characterization and colloid release were conducted. The field tests were carried out at an artificial recharge test site in Shandong Province. In the field investigation, one recharge water sample and five groundwater samples were collected and filtered through three levels of ultrafiltration membranes, with pore sizes of 0...
April 24, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28439034/teleosts-as-model-organisms-to-understand-host-microbe-interactions
#4
Emily A Lescak, Kathryn C Milligan-Myhre
Host-microbe interactions are influenced by complex host genetics and environment. Studies across animal taxa have aided our understanding of how intestinal microbiota influence vertebrate development, disease, and physiology. However, traditional mammalian studies can be limited by use of isogenic strains, husbandry constraints that result in small sample sizes and limited statistical power, reliance on indirect characterization of gut microbial communities from fecal samples, and concerns whether observations in artificial conditions are actually reflective of what occurs in the wild...
April 24, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28436648/statistical-variable-selection-an-alternative-prioritization-strategy-during-the-non-target-analysis-of-lc-hr-ms-data
#5
Saer Samanipour, Malcolm J Reid, Kevin V Thomas
Liquid chromatography coupled to high resolution mass spectrometry (LC-HR-MS) has been one of the main analytical tools for the analysis of small polar organic pollutants in the environment. LC-HR-MS typically produces a large amount of data for a single chromatogram. The analyst is therefore required to perform prioritization prior to non-target structural elucidation. In the present study we have combined the F-ratio statistical variable selection and the apex detection algorithms in order to perform prioritization in data sets produced via LC-HR-MS...
April 24, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28434057/predictive-control-of-intersegmental-tarsal-movements-in-an-insect
#6
Alicia Costalago-Meruelo, David M Simpson, Sandor M Veres, Philip L Newland
In many animals intersegmental reflexes are important for postural and movement control but are still poorly undesrtood. Mathematical methods can be used to model the responses to stimulation, and thus go beyond a simple description of responses to specific inputs. Here we analyse an intersegmental reflex of the foot (tarsus) of the locust hind leg, which raises the tarsus when the tibia is flexed and depresses it when the tibia is extended. A novel method is described to measure and quantify the intersegmental responses of the tarsus to a stimulus to the femoro-tibial chordotonal organ...
April 22, 2017: Journal of Computational Neuroscience
https://www.readbyqxmd.com/read/28433878/effect-of-freshwater-sediment-characteristics-on-the-persistence-of-fecal-indicator-bacteria-and-genetic-markers-within-a-southern-california-watershed
#7
Amity G Zimmer-Faust, Vanessa Thulsiraj, Catalina Marambio-Jones, Yiping Cao, John F Griffith, Patricia A Holden, Jennifer A Jay
In this study, the aging of culturable FIB and DNA representing genetic markers for Enterococcus spp. (ENT1A), general Bacteroides (GB3), and human-associated Bacteroides (HF183) in freshwater sediments was evaluated. Freshwater sediment was collected from four different sites within the upper and lower reach of the Topanga Creek Watershed and two additional comparator sites within the Santa Monica Bay, for a total of six sites. Untreated (ambient) and oven-dried (reduced microbiota) sediment was inoculated with 5% sewage and artificial freshwater...
April 18, 2017: Water Research
https://www.readbyqxmd.com/read/28430205/controlled-membrane-translocation-provides-a-mechanism-for-signal-transduction-and-amplification
#8
Matthew J Langton, Flore Keymeulen, Maria Ciaccia, Nicholas H Williams, Christopher A Hunter
Transmission and amplification of chemical signals across lipid bilayer membranes is of profound significance in many biological processes, from the development of multicellular organisms to information processing in the nervous system. In biology, membrane-spanning proteins are responsible for the transmission of chemical signals across membranes, and signal transduction is often associated with an amplified signalling cascade. The ability to reproduce such processes in artificial systems has potential applications in sensing, controlled drug delivery and communication between compartments in tissue-like constructs of synthetic vesicles...
May 2017: Nature Chemistry
https://www.readbyqxmd.com/read/28429314/evolutionary-origin-of-euglena
#9
Bożena Zakryś, Rafał Milanowski, Anna Karnkowska
Euglenids (Excavata, Discoba, Euglenozoa, Euglenida) is a group of free-living, single-celled flagellates living in the aquatic environments. The uniting and unique morphological feature of euglenids is the presence of a cell covering called the pellicle. The morphology and organization of the pellicle correlate well with the mode of nutrition and cell movement. Euglenids exhibit diverse modes of nutrition, including phagotrophy and photosynthesis. Photosynthetic species (Euglenophyceae) constitute a single subclade within euglenids...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28427684/rapid-and-high-capacity-ultrasonic-assisted-adsorption-of-ternary-toxic-anionic-dyes-onto-mof-5-activated-carbon-artificial-neural-networks-partial-least-squares-desirability-function-and-isotherm-and-kinetic-study
#10
Hanieh Askari, Mehrorang Ghaedi, Kheibar Dashtian, Mohammad Hossein Ahmadi Azghandi
The present paper focused on the ultrasonic assisted simultaneous removal of fast green (FG), eosin Y (EY) and quinine yellow (QY) from aqueous media following using MOF-5 as a metal organic framework and activated carbon hybrid (AC-MOF-5). The structure and morphology of AC-MOF-5 was identified by SEM, FTIR and XRD analysis. The interactive and main effects of variables such as pH, initial dyes concentration (mgL(-1)), adsorbent dosage (mg) and sonication time (min) on removal percentage were studied by central composite design (CCD), subsequent desirability function (DF) permit to achieved real variable experimental condition...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28426800/neutrophil-lymphocyte-ratio-predicts-short-term-mortality-in-patients-with-hepatitis-b-virus-related-acute-on-chronic-liver-failure-treated-with-an-artificial-liver-support-system
#11
Zeng Fan, Chen EnQiang, Du Ling Yao, Yan LiBo, Li Hong, Bai Lang, Feng Ping, Tang Hong
AIM: Hepatitis B virus-related acute-on-chronic liver failure has high short-term mortality. Artificial liver support systems (ALSS) may improve outcome and avoid liver transplantation, but predicting short-term prognosis in such patients is difficult. This study aimed to determine whether the neutrophil-lymphocyte ratio (NLR), an inflammation marker, predicted mortality in patients treated with ALSS. METHODS: A total of 560 patients with hepatitis B virus-related acute-on-chronic liver failure were enrolled, 338 were treated with ALSS and the others treated with standard of care(SOC)...
2017: PloS One
https://www.readbyqxmd.com/read/28424669/inferring-microbial-interactions-in-the-gut-of-the-hong-kong-whipping-frog-polypedates-megacephalus-and-a-validation-using-probiotics
#12
Francis Cheng-Hsuan Weng, Grace Tzun-Wen Shaw, Chieh-Yin Weng, Yi-Ju Yang, Daryi Wang
The concerted activity of intestinal microbes is crucial to the health and development of their host organisms. Investigation of microbial interactions in the gut should deepen our understanding of how these micro-ecosystems function. Due to advances in Next Generation Sequencing (NGS) technologies, various bioinformatic strategies have been proposed to investigate these microbial interactions. However, due to the complexity of the intestinal microbial community and difficulties in monitoring their interactions, at present there is a gap between the theory and biological application...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28420257/gestational-diabetes-management-using-smart-mobile-telemedicine
#13
Mercedes Rigla, Iñaki Martínez-Sarriegui, Gema García-Sáez, Belén Pons, Maria Elena Hernando
Gestational diabetes (GDM) burden has been increasing progressively over the past years. Knowing that intrauterine exposure to maternal diabetes confers high risk for macrosomia as well as for future type 2 diabetes and obesity of the offspring, health care organizations try to provide effective control in spite of the limited resources. Artificial-intelligence-augmented telemedicine has been proposed as a helpful tool to facilitate an efficient widespread medical assistance to GDM. The aim of the study we present was to test the feasibility and acceptance of a mobile decision-support system for GDM, developed in the seventh framework program MobiGuide Project, which includes computer-interpretable clinical practice guidelines, access to data from the electronic health record as well as from glucose, blood pressure, and activity sensors...
April 1, 2017: Journal of Diabetes Science and Technology
https://www.readbyqxmd.com/read/28419946/water-immiscible-bioinert-coatings-and-film-formation-from-aqueous-dispersions-of-poly-2-methoxyethyl-acrylate-microspheres
#14
Takuma Kureha, Seina Hiroshige, Shusuke Matsui, Daisuke Suzuki
Poly(2-methoxyethyl acrylate) (pMEA) microspheres are prepared through facile free-radical polymerization in water without additives and impurities, such as surfactants, other polymers, and organic solvents, which are usually used to synthesize pMEA chains. Clean and pure (non-factionalized and non-cross-linking) pMEA microspheres exhibit plasma-protein adsorption resistances on their surface regardless of their charged state. They are characterized in terms of the adsorbed amounts of proteins at pH 7. In addition, these soft and deformable pMEA microspheres are suitable for forming substrates coated with pMEA microspheres and free-standing films by injecting pMEA dispersion and evaporating the aqueous medium through fusion between the pMEA chains at the surface without the precoating agent and cross-linker...
April 10, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28419477/the-effectiveness-of-riboflavin-and-ultraviolet-light-pathogen-reduction-technology-in-eliminating-trypanosoma-cruzi-from-leukoreduced-whole-blood
#15
Teresa Jimenez-Marco, Beatriz Cancino-Faure, Enrique Girona-Llobera, M Magdalena Alcover, Cristina Riera, Roser Fisa
BACKGROUND: The parasitic Chagas disease is caused by the protozoan Trypanosoma cruzi, which is mainly transmitted by insect vectors. Other infection routes, both in endemic and in nonendemic areas, include organ and marrow transplantation, congenital transmission, and blood transfusion. Asymptomatic chronic chagasic individuals may have a low and transient parasitemia in peripheral blood and, consequently, they can unknowingly transmit the disease via blood transfusion. Riboflavin and ultraviolet (UV) light pathogen reduction is a method to reduce pathogen transfusion transmission risk based on damage to the pathogen nucleic acids...
April 16, 2017: Transfusion
https://www.readbyqxmd.com/read/28419148/how-long-to-rest-in-unpredictably-changing-habitats
#16
Mirosław Slusarczyk, Jacek Starzyński, Piotr Bernatowicz
In the present study, we investigated the optimum length of prolonged dormancy (developmental arrest extending over favourable periods) of organisms under uncertain environmental conditions. We used an artificial life model to simulate the evolution of suspended development in the ontogenesis of organisms inhabiting unpredictably changing habitats. A virtual population of semelparous parthenogenetic individuals that varied in a duration of developmental arrest competed for limited resources. At a constant level of available resources, uninterrupted development was the superior life strategy...
2017: PloS One
https://www.readbyqxmd.com/read/28418080/phototoxic-potential-of-undispersed-and-dispersed-fresh-and-weathered-macondo-crude-oils-to-gulf-of-mexico-marine-organisms
#17
Bryson E Finch, Solmaz Marzooghi, Dominic M Di Toro, William A Stubblefield
Crude oils contain a mixture of hydrocarbons, including phototoxic polycyclic aromatic hydrocarbons (PAHs) that have the ability to absorb ultraviolet (UV) light. Absorption of UV light by PAHs can substantially increase their toxicity to marine organisms. The objective of the present study was to examine the potential for phototoxicity of fresh and naturally weathered Macondo crude oils alone and in combination with the dispersant Corexit 9500 to mysid shrimp (Americamysis bahia), inland silverside (Menidia beryllina), sheepshead minnow (Cyprinodon variegatus), and Gulf killifish (Fundulus grandis)...
April 18, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28417363/carbohydrate-depolymerization-by-intricate-cellulosomal-systems
#18
Johanna Stern, Lior Artzi, Sarah Moraïs, Carlos M G A Fontes, Edward A Bayer
Cellulosomes are multi-enzymatic nanomachines that have been fine-tuned through evolution to efficiently deconstruct plant biomass. Integration of cellulosomal components occurs via highly ordered protein-protein interactions between the various enzyme-borne dockerin modules and the multiple copies of the cohesin modules located on the scaffoldin subunit. Recently, designer cellulosome technology has been established to provide insights into the architectural role of catalytic (enzymatic) and structural (scaffoldin) cellulosomal constituents for the efficient degradation of plant cell wall polysaccharides...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28416316/effect-of-passive-ultrasonic-activation-on-organic-tissue-dissolution-from-simulated-grooves-in-root-canals-using-sodium-hypochlorite-with-or-without-surfactants-and-edta
#19
Roberto Estevez, Antonio J Conde, Oliver Valencia de Pablo, Francisco de la Torre, Giampiero Rossi-Fedele, Rafael Cisneros
INTRODUCTION: The aim of this study was to evaluate the porcine palatal mucosa dissolution from artificial grooves using a final rinse with sodium hypochlorite (NaOCl) with or without a surfactant or ultrasonic activation (PUI). METHODS: The root canals of 130 human maxillary central incisors were chemomechanically prepared and the teeth split. A standardized longitudinal intracanal groove was created in 1 of the root halves. One hundred thirty porcine palatal mucosa samples were collected, adapted in order to fit into the grooves, and weighed...
April 14, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28415237/latent-geometry-of-bipartite-networks
#20
Maksim Kitsak, Fragkiskos Papadopoulos, Dmitri Krioukov
Despite the abundance of bipartite networked systems, their organizing principles are less studied compared to unipartite networks. Bipartite networks are often analyzed after projecting them onto one of the two sets of nodes. As a result of the projection, nodes of the same set are linked together if they have at least one neighbor in common in the bipartite network. Even though these projections allow one to study bipartite networks using tools developed for unipartite networks, one-mode projections lead to significant loss of information and artificial inflation of the projected network with fully connected subgraphs...
March 2017: Physical Review. E
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