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Fibroblast Grow Factor

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https://www.readbyqxmd.com/read/30502050/generation-of-renal-epo-producing-cell-lines-by-conditional-gene-tagging-reveals-rapid-hif-2-driven-epo-kinetics-cell-autonomous-feedback-regulation-and-a-telocyte-phenotype
#1
Faik Imeri, Karen A Nolan, Andreas M Bapst, Sara Santambrogio, Irene Abreu-Rodríguez, Patrick Spielmann, Svende Pfundstein, Silvana Libertini, Lisa Crowther, Ilaria M C Orlando, Sophie L Dahl, Anna Keodara, Willy Kuo, Vartan Kurtcuoglu, Carsten C Scholz, Weihong Qi, Edith Hummler, David Hoogewijs, Roland H Wenger
Erythropoietin (Epo) is essential for erythropoiesis and is mainly produced by the fetal liver and the adult kidney following hypoxic stimulation. Epo regulation is commonly studied in hepatoma cell lines, but differences in Epo regulation between kidney and liver limit the understanding of Epo dysregulation in polycythaemia and anaemia. To overcome this limitation, we have generated a novel transgenic mouse model expressing Cre recombinase specifically in the active fraction of renal Epo-producing (REP) cells...
November 23, 2018: Kidney International
https://www.readbyqxmd.com/read/30455427/the-klotho-proteins-in-health-and-disease
#2
REVIEW
Makoto Kuro-O
The Klotho proteins, αKlotho and βKlotho, are essential components of endocrine fibroblast growth factor (FGF) receptor complexes, as they are required for the high-affinity binding of FGF19, FGF21 and FGF23 to their cognate FGF receptors (FGFRs). Collectively, these proteins form a unique endocrine system that governs multiple metabolic processes in mammals. FGF19 is a satiety hormone that is secreted from the intestine on ingestion of food and binds the βKlotho-FGFR4 complex in hepatocytes to promote metabolic responses to feeding...
November 19, 2018: Nature Reviews. Nephrology
https://www.readbyqxmd.com/read/30429125/development-and-characterization-of-a-new-cell-line-derived-from-european-eel-anguilla-anguilla-kidney
#3
Bin Chen, Zaiyu Zheng, Jinxian Yang, Hongshu Chi, He Huang, Hui Gong
A new cell line derived from the kidney of European eel, Anguilla anguilla , has been established and characterized. This cell line, designated as EK, has been maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum for over 24 months, and subcultured more than 60 times. This cell line consists predominantly of fibroblast-like cells, and could grow at 15-37°C under an optimum temperature of 26°C. The origin of this cell line was confirmed by polymerase chain reaction (PCR) amplification and 18s recombinant (r)RNA sequencing...
November 14, 2018: Biology Open
https://www.readbyqxmd.com/read/30380963/technical-and-diagnostic-performance-of-a-new-fully-automated-immunoassay-for-the-determination-of-intact-fibroblast-growth-factor-23-fgf23
#4
Josef van Helden, Ralf Weiskirchen
There is growing interest in measuring plasma fibroblast growth factor 23 (FGF23) concentrations in a number of clinical settings. However, a reliable assay with acceptable performance is lacking. Plasma samples of healthy adults and patients with different stages of chronic kidney disease (CKD) were used to compare the precision, recovery, linearity and the pre-analytical stability characteristics of a new fully automated FGF23 (intact) assay with a commercially available FGF23 (intact) ELISA. Method agreement was evaluated, reference and stage-specific ranges for kidney disease were established...
October 31, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/30355943/fgfr2-promotes-gastric-cancer-progression-by-inhibiting-the-expression-of-thrombospondin4-via-pi3k-akt-mtor-pathway
#5
Tingting Huang, Dian Liu, Yihua Wang, Piao Li, Li Sun, Huihua Xiong, Yuhong Dai, Man Zou, Xianglin Yuan, Hong Qiu
BACKGROUND/AIMS: Fibroblast growth factor receptor 2 (FGFR2) has attracted considerable interest as a therapeutic target in gastric cancer (GC). There is growing evidence to suggest that the bioavailability of the potent pro-tumor function of FGFR2 is associated with thrombospondins (TSPs). As a follow-on from our previous study, here we evaluated the potential clinical significance and mechanism of the relationship between FGFR2 and TSP4 in GC. METHODS: Expression levels of FGFR2 and TSP4 were detected by immunohistochemistry in GC tissue microarray slides...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/30348174/macrophage-migration-inhibitory-factor-contributes-to-the-pathogenesis-of-benign-lymphoepithelial-lesion-of-the-lacrimal-gland
#6
Yao Mawulikplimi Adzavon, Pengxiang Zhao, Jianmin Ma, Xujuan Zhang, Xin Zhang, Mingzi Zhang, Mengyu Liu, Limin Wang, Danying Chen, Tarekegn Gebreyesus Abisso, Baobei Lv, Lei Wang, Fei Xie, Xuemei Ma
BACKGROUND: Benign Lymphoepithelial Lesion (BLEL) is a rare disease observed in the adult population. Despite the growing numbers of people suffering from BLEL, the etiology and mechanisms underlying its pathogenesis remain unknown. METHODS: In the present study, we used gene and cytokines expression profiling, western blot and immunohistochemistry to get further insight into the cellular and molecular mechanisms involved in the pathogenesis of BLEL of the lacrimal gland...
October 22, 2018: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/30297626/roles-of-gut-derived-secretory-factors-in-the-pathogenesis-of-non-alcoholic-fatty-liver-disease-and-their-possible-clinical-applications
#7
REVIEW
Hirofumi Okubo, Akifumi Kushiyama, Yusuke Nakatsu, Takeshi Yamamotoya, Yasuka Matsunaga, Midori Fujishiro, Hideyuki Sakoda, Haruya Ohno, Masayasu Yoneda, Tomoichiro Asano
The rising prevalence of non-alcoholic fatty liver disease (NAFLD) parallels the global increase in the number of people diagnosed with obesity and metabolic syndrome. The gut-liver axis (GLA) plays an important role in the pathogenesis of NAFLD/non-alcoholic steatohepatitis (NASH). In this review, we discuss the clinical significance and underlying mechanisms of action of gut-derived secretory factors in NAFLD/NASH, focusing on recent human studies. Several studies have identified potential causal associations between gut-derived secretory factors and NAFLD/NASH, as well as the underlying mechanisms...
October 8, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30268800/the-role-of-kinins-in-the-proliferation-of-fibroblast-primed-with-tnf-in-scratch-wound-assay-kinins-and-cell-proliferation
#8
Ana Julia Von Borell du Vernay França, Renata De Faveri, Roberta Nunes, Viviane Miranda Bispo Steimbach, José Roberto Santin, Nara Lins Meira Quintão
The aim of this study was to evaluate the involvement of both B1 and B2 kinins receptors (B1 R and B2 R) in the fibroblast proliferation induced by the cytokine tumour necrosis factor (TNF) attempting to establish an in vitro model of wound healing. Murine fibroblasts L-929 were cultivated in 24 wells plaque until total confluence (DMEM (Vitrocell®); 5% fetal bovine serum, 5% CO2 , 37 °C) and then submitted to the scratch assay. The cells were treated with PBS, TNF (2 ng/mL) and/or mr-TNF antibody (200 μg/mL), or PDTC...
December 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/30250574/smokeless-tobacco-extract-inhibits-proliferation-and-promotes-apoptosis-in-oral-mucous-fibroblasts
#9
Lei Li, Xiaoqing Zhou, Yanran Wang
The consumption of smokeless tobacco extract (STE) is growing rapidly, and it has been implicated in several human diseases including diabetes, inflammation and a number of types of cancer. The toxicity of STE requires evaluation, as it is known to induce numerous public health issues. To investigate whether STE serves a role in cultured human oral mucosa fibroblasts (hOMFs), the present study examined HOMF morphology with inverted microscopy and immunofluorescence staining. The cell viability was measured with MTT assays, which detected the cell apoptosis rate via flow cytometry...
October 2018: Oncology Letters
https://www.readbyqxmd.com/read/30213304/-bone-and-metabolism
#10
Charlotte Marsot, Philippe Chanson, Françoise Borson-Chazot
Bone is now considered as a particular endocrine organ. Its endocrine function is not yet fully understood and has been the subject of several conferences at the European Society of Endocrinology Congress 2018. Bone regulates phosphate metabolism by secreting fibroblast growth factor 23; it also regulates glucose metabolism via osteocalcin and energy metabolism, thanks to lipocalin 2, a new hormone acting on the brain. In addition, the incidence of diabetes continues to grow, and its impact on bone has been demonstrated, with an increased risk of fractures regardless the type of diabetes...
September 2018: Annales D'endocrinologie
https://www.readbyqxmd.com/read/30177133/autologous-and-allogeneic-skin-cell-grafts-in-the-treatment-of-severely-burned-patients-retrospective-clinical-study
#11
COMPARATIVE STUDY
A Klama-Baryła, D Kitala, W Łabuś, M Kraut, J Glik, M Nowak, M Kawecki
BACKGROUND: Transplantation of skin cells (keratinocytes and fibroblasts) cultured in vitro is a method of choice for the treatment of severe and extensive burns in patients with a deficit of donor sites for free split-thickness skin grafts, and when the grave medical condition of the patient excludes the possibility of an operation under general anesthetic. Appropriate amounts of keratinocytes and/or fibroblasts cultured in vitro are grafted as a suspension in platelet-leukocyte-rich gel directly on the prepared acceptor site...
September 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/30142919/the-effect-of-sea-buckthorn-hippophae-rhamnoides-l-seed-oil-on-uv-induced-changes-in-lipid-metabolism-of-human-skin-cells
#12
Agnieszka Gęgotek, Anna Jastrząb, Iwona Jarocka-Karpowicz, Marta Muszyńska, Elżbieta Skrzydlewska
Lipids and proteins of skin cells are the most exposed to harmful ultraviolet (UV) radiation contained in sunlight. There is a growing need for natural compounds that will protect these sensitive molecules from damage, without harmful side effects. The aim of this study was to investigate the effect of sea buckthorn seed oil on the redox balance and lipid metabolism in UV irradiated cells formed different skin layers to examine whether it had a protective effect. Human keratinocytes and fibroblasts were subjected to UVA (ultraviolet type A; 30 J/cm² and 20 J/cm²) or UVB (ultraviolet type B; 60 mJ/cm² and 200 mJ/cm², respectively) radiation and treated with sea buckthorn seed oil (500 ng/mL), and the redox activity was estimated by reactive oxygen species (ROS) generation and enzymatic/non-enzymatic antioxidants activity/level (using electron spin resonance (ESR), high-performance liquid chromatography (HPLC), and spectrophotometry)...
August 23, 2018: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/30130563/developmental-pathways-in-the-pathogenesis-of-lung-fibrosis
#13
REVIEW
Diptiman Chanda, Eva Otoupalova, Samuel R Smith, Thomas Volckaert, Stijn P De Langhe, Victor J Thannickal
Idiopathic pulmonary fibrosis (IPF) is a progressive and terminal lung disease with no known cure. IPF is a disease of aging, with median age of diagnosis over 65 years. Median survival is between 3 and 5 years after diagnosis. IPF is characterized primarily by excessive deposition of extracellular matrix (ECM) proteins by activated lung fibroblasts and myofibroblasts, resulting in reduced gas exchange and impaired pulmonary function. Growing evidence supports the concept of a pro-fibrotic environment orchestrated by underlying factors such as genetic predisposition, chronic injury and aging, oxidative stress, and impaired regenerative responses may account for disease development and persistence...
August 22, 2018: Molecular Aspects of Medicine
https://www.readbyqxmd.com/read/30121527/the-effects-of-elevated-fibroblast-growth-factor-23-on-mandibular-growth-in-rats
#14
Yanan Wu, Jinkai Zhang, Fengshan Chen
OBJECTIVE: The aim of this study is to elucidate the local effects of fibroblast growth factor 23 (FGF23) in on mandibular condylar growth in growing rats. DESIGN: Growing Sprague-Dawley rats received intra-temporomandibular joint injections of phosphate buffer solution (PBS), adenovirus-mediated green fluorescent protein (Ad-GFP) or adenovirus-mediated fibroblast growth factor 23 (Ad-FGF23), which were marked as groups A, B, and C, respectively. In vitro, we treated rat mandibular cartilage chondrocytes with PBS, Ad-GFP, and Ad-FGF23...
August 1, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/30118759/factor-xii-in-coagulation-inflammation-and-beyond
#15
Miroslava Didiasova, Lukasz Wujak, Liliana Schaefer, Malgorzata Wygrecka
Factor XII (FXII) is a protease that is mainly produced in the liver and circulates in plasma as a single chain zymogen. Following contact with negatively charged surfaces, FXII is converted into the two-chain active form, FXIIa. FXIIa initiates the intrinsic blood coagulation pathway via activation of factor XI. Furthermore, it converts plasma prekallikrein to kallikrein (PK), which reciprocally activates FXII and liberates bradykinin from high molecular weight kininogen. In addition, FXIIa initiates fibrinolysis via PK-mediated urokinase activation and activates the classical complement pathway...
November 2018: Cellular Signalling
https://www.readbyqxmd.com/read/30094610/cxcl1-derived-from-mammary-fibroblasts-promotes-progression-of-mammary-lesions-to-invasive-carcinoma-through-cxcr2-dependent-mechanisms
#16
Shira Bernard, Megan Myers, Wei Bin Fang, Brandon Zinda, Curtis Smart, Diana Lambert, An Zou, Fang Fan, Nikki Cheng
With improved screening methods, the numbers of abnormal breast lesions diagnosed in women have been increasing over time. However, it remains unclear whether these breast lesions will develop into invasive cancers. To more effectively predict the outcome of breast lesions and determine a more appropriate course of treatment, it is important to understand the underlying mechanisms that regulate progression of non-invasive lesions to invasive breast cancers. A hallmark of invasive breast cancers is the accumulation of fibroblasts...
December 2018: Journal of Mammary Gland Biology and Neoplasia
https://www.readbyqxmd.com/read/30042343/the-tumor-microenvironment-of-epithelial-ovarian-cancer-and-its-influence-on-response-to-immunotherapy
#17
REVIEW
Galaxia M Rodriguez, Kristianne J C Galpin, Curtis W McCloskey, Barbara C Vanderhyden
Immunotherapy as a treatment for cancer is a growing field of endeavor but reports of success have been limited for epithelial ovarian cancer. Overcoming the challenges to developing more effective therapeutic approaches lies in a better understanding of the factors in cancer cells and the surrounding tumor microenvironment that limit response to immunotherapies. This article provides an overview of some ovarian cancer cell features such as tumor-associated antigens, ovarian cancer-derived exosomes, tumor mutational burden and overexpression of immunoinhibitory molecules...
July 24, 2018: Cancers
https://www.readbyqxmd.com/read/30038941/an-integrated-approach-toward-the-biomanufacturing-of-engineered-cell-therapy-products-in-a-stirred-suspension-bioreactor
#18
Charlie Y M Hsu, Tylor Walsh, Breanna S Borys, Michael S Kallos, Derrick E Rancourt
Recent advances in stem cell biology have accelerated the pre-clinical development of cell-based therapies for degenerative and chronic diseases. The success of this growing area hinges upon the concomitant development of scalable manufacturing platforms that can produce clinically relevant quantities of cells for thousands of patients. Current biomanufacturing practices for cell therapy products are built on a model previously optimized for biologics, wherein stable cell lines are established first, followed by large-scale production in the bioreactor...
June 15, 2018: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/30033985/gdf9-and-bmp15-induce-development-of-antrum-like-structures-by-bovine-granulosa-cells-without-oocytes
#19
Md Hasanur Alam, Jibak Lee, Takashi Miyano
The role of oocytes in follicular antrum formation is not well understood. We examined the effect of oocyte-derived growth factors, growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15), on the formation of antrum-like structures by cultured bovine oocyte-granulosa cell complexes (OGCs). OGCs containing growing oocytes (105‒115 µm in diameter) were collected from early antral follicles (1.2‒1.8 mm) and used to prepare oocytectomized complexes (OXCs) and granulosa cell complexes (GCs)...
October 12, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/30029518/fgf2-dual-warhead-conjugate-with-monomethyl-auristatin-e-and-%C3%AE-amanitin-displays-a-cytotoxic-effect-towards-cancer-cells-overproducing-fgf-receptor-1
#20
Karolina Weronika Świderska, Anna Szlachcic, Łukasz Opaliński, Małgorzata Zakrzewska, Jacek Otlewski
In the rapidly developing field of targeted cancer therapy there is growing interest towards therapeutics combining two or more compounds to achieve synergistic action and minimize the chance of cancer resistance to treatment. We developed a fibroblast growth factor 2 (FGF2)-conjugate bearing two cytotoxic drugs with independent mode of action: α-amanitin and monomethyl auristatin E. Drugs are covalently attached to the targeting protein in a site-specific manner via maleimide-thiol conjugation and Cu(I)-catalyzed alkyne-azide cycloaddition...
July 19, 2018: International Journal of Molecular Sciences
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