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Sonia Emanuele, Antonietta Notaro, Antonio Palumbo Piccionello, Antonella Maggio, Marianna Lauricella, Antonella D'Anneo, Cesare Cernigliaro, Giuseppe Calvaruso, Michela Giuliano
Litchi chinensis Sonnerat is a tropical tree whose fruits contain significant amounts of bioactive polyphenols. Litchi cultivation has recently spread in Sicily where the climate conditions are particularly favorable for this crop. Recent findings have shown that Litchi extracts display anti-tumor and pro-apoptotic effects in vitro, but the precise underlying mechanisms have not been fully elucidated. In this study, we report for the first time the effects of Sicilian litchi fruit extracts on colon cancer cells...
October 12, 2018: Nutrients
Lei Ding, Huan Gu, Zhenwei Lan, Qingchun Lei, Wenxue Wang, Jiangfei Ruan, Min Yu, Jie Lin, Qinghua Cui
The prognosis of patients with colorectal cancer (CRC) remains poor owing to diagnosis typically occurring at advanced stages of the disease. The understanding of the molecular regulatory signatures of CRC may lead to the identification of biomarkers for the early detection, prevention and clinical intervention of CRC. Epidemiological studies have indicated that cyclooxygenase‑1 (COX‑1) serves an active function in colon carcinogenesis. However, the molecular mechanism underlying COX‑1 regulation in CRC remains unknown...
October 12, 2018: Oncology Reports
Issara Kaweewan, Hisayuki Komaki, Hikaru Hemmi, Kanata Hoshino, Takeshi Hosaka, Gouchi Isokawa, Takanori Oyoshi, Shinya Kodani
Using genome mining, a new cytotoxic peptide named curacozole was isolated from Streptomyces curacoi. Through ESI-MS and NMR analyses, curacozole was determined to be a macrocyclic peptide containing two isoleucine, two thiazole and three oxazole moieties. Curacozole exhibited potent cytotoxic activity against HCT116 and HOS cancer cells. The proposed biosynthetic gene cluster of curacozole was identified and compared with that of the related compound YM-216391.
October 11, 2018: Journal of Antibiotics
Hai Li, Shikuo Rong, Chao Chen, Yayun Fan, Tuo Chen, Yong Wang, Dongmei Chen, Chun Yang, Jiali Yang
Human C-X-C Motif Chemokine Receptor 3A (CXCR3A) and CXCR3B are two splice variants of CXCR3 that is involved in a variety of progressive processes of cancer cells, including proliferation, migration, invasion, and tumorigenicity. However, the molecular mechanisms of CXCR3 in colorectal cancer (CRC) remain incomplete understood. In the present study, a significantly up-regulated CXCR3 protein was firstly observed in CRC tissues and cell lines in comparison with the paired non-tumor tissues and normal intestinal epithelial cells, which was positively associated with CRC TNM stages...
October 9, 2018: Molecular Carcinogenesis
Alexandre Vernay, Otmane Lamrabet, Jackie Perrin, Pierre Cosson
Previous studies have shown that TM9SF4 interacts with glycine-rich transmembrane domains (TMDs) and promotes their surface localization, presumably by escorting them along the secretory pathway. Our results indicate that TM9SF4 interacts with and sorts a variety of TMDs. In HEK cells, a TMD carrying a positively charged residue (T-R1) or a negatively charged residue (T-D1) was localized in the ER, but was partially relocated to the Golgi complex upon overexpression of TM9SF4. These results show that TM9SF4 controls the sorting of TMDs at the ER-Golgi interface...
October 9, 2018: Journal of Cell Science
Wei Wu, Bing Hou, Changli Tang, Fucheng Liu, Jie Yang, Tao Pan, Ke Si, Deyun Lu, Xiaoxiang Wang, Jing Wang, Xing Xiong, Ji Liu, Chunguang Xie
Inhibition of tumor cell migration is a treatment strategy for patients with colorectal cancer (CRC). SCF-dependent activation of c-KIT is responsible for migration of c-KIT positive [c-KIT(+)] cells of CRC. Drug resistance to Imatinib Mesylate (c-KIT inhibitor) has emerged. Inhibition of mTOR can induce autophagic degradation of c-KIT. (+)-usnic acid [(+)-UA], isolated from lichens, has two major functions including induction of proton shuttle and targeting inhibition of mTOR. To reduce hepatotoxicity, the treatment concentration of (+)-UA should be lower than 10 μ M...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Tanize Acunha, Virginia García-Cañas, Alberto Valdés, Alejandro Cifuentes, Carolina Simó
Rosemary diterpenes have demonstrated diverse biological activities, such as anti-cancer, antiinflammatory, as well as other beneficial effects against neurological and metabolic disorders. In particular, carnosic acid (CA), carnosol (CS) and rosmanol (RS) diterpenes have shown interesting results on anti-cancer activity. However, little is known about the toxic effects of rosemary diterpenes at the concentrations needed to exert their antiproliferative effect on cancer cells. In our study, CA, CS and RS exhibited a concentration-dependent effect on cell viability of two human colon cancer cell lines (HT-29 and HCT116) after 24 h exposure...
December 11, 2018: Analytica Chimica Acta
Xingshu Li, C-Yoon Kim, Jeong Min Shin, Dayoung Lee, Gyoungmi Kim, Hyung-Min Chung, Ki-Sung Hong, Juyoung Yoon
Precise targeting with minimal side effects is of particular interest for personalized medicine, although it remains a challenge. Herein, we demonstrate precision photodynamic therapy (PDT) utilizing human mesenchymal stem cells (MSCs) as cellular vehicles to deliver a new activatable photosensitizer (PcS). In vivo real-time optical imaging tests indicated that PcS-loaded MSCs possess excellent tumor-homing properties. More importantly, dye transfer assays confirm that MSCs precisely transfer PcS into human colon cancer cells (HCT116) via the "bystander effect...
September 28, 2018: Biomaterials
An-Dong Wang, Yun-Qiang Zhang, Mei-Chen Li, Xia Wang, Bin Lin, Jian-Yu Liu, Yong-Nan Xu
Zeasesquiterpene A-E (1-5), five new sesquiterpenes with two cyclohexanes, were isolated from the roots of Zea mays. Their structures were elucidated on the basis of 1D and 2D NMR spectral data and ECD analysis. A plausible biosynthetic pathway for the compounds (1-6) were hypothesized. All isolated compounds were screened for cytotoxicities against five human cancer cell lines (A549, MDA-MB-231, SK-Hep-1, SNU638 and HCT116) in vitro by MTT assay. Compound 4 showed potential cytotoxic activities against A549 (14...
October 2, 2018: Fitoterapia
Kevin W Wellington, Natasha I Kolesnikova, Nomgqibelo B P Nyoka, Lyndy J McGaw
In this study, we report on the inhibitory activity of synthesized aminonaphthoquinones against two bacterial and one fungal species to determine their antimicrobial properties. A minimum inhibitory concentration (MIC) of 7.8 μg/mL was obtained against the fungus, Candida albicans, which was better than that of Amphotericin B (MIC = 31.25 μg/mL). Escherichia coli (Gram -), was inhibited at a MIC of 23.4 μg/mL and Staphylococcus aureus (Gram +) at a MIC of 31.3 μg/mL. The aminonaphthoquinones were also screened against HCT116 colon, PC3 prostate and HepG2 liver cancer cell lines to evaluate their cytostatic effects...
October 4, 2018: Drug Development Research
Hao Qi, Xiaoyan Shi, Miao Yu, Boya Liu, Minghui Liu, Shi Song, Shuaiyi Chen, Junhua Zou, Wei-Guo Zhu, Jianyuan Luo
The histone transmethylase complex comprising WD repeat domain 77 (WDR77) and protein arginine methyltransferase 5 (PRMT5) catalyzes dimethylation of H4R3 (H4R3me2) and drives cancer cell proliferation and migration, but its regulation is not fully understood. Here, we report that sirtuin 7 (SIRT7) directly deacetylates WDR77 and that this deacetylation interferes with the WDR77-PRMT5 interaction and suppresses proliferation of human colon cancer HCT116 cells. Using co-expression in HEK293T cells and co-immunoprecipitation assays, we observed that SIRT7 deacetylates WDR77 at Lys-3 and Lys-243, which reduced of WDR77's interaction with PRMT5...
October 3, 2018: Journal of Biological Chemistry
Cho R Hong, Gib Bogle, Jingli Wang, Kashyap Patel, Frederik B Pruijn, William R Wilson, Kevin O Hicks
Intra-tumor heterogeneity represents a major barrier to anti-cancer therapies. One strategy to minimize this limitation relies on bystander effects via diffusion of cytotoxins from targeted cells. Hypoxia-activated prodrugs (HAPs) have the potential to exploit hypoxia in this way, but robust methods for measuring bystander effects are lacking. The objective of this study is to develop experimental models (monolayer, multilayer, and multicellular spheroid co-cultures) comprising 'activator' cells with high expression of prodrug-activating reductases and reductase-deficient 'target' cells, and to couple these with agent-based models (ABMs) that describe diffusion and reaction of prodrugs and their active metabolites, and killing probability for each cell...
2018: Frontiers in Pharmacology
Chang-Feng Li, Yong-Chao Li, Yun Wang, Li-Bo Sun
BACKGROUND/AIMS: Since the combined actions of lncRNAs and miRNAs have been considered to be involved in the occurrence and development of various neoplasms, the main purpose of this study was to discover whether and how lncRNA H19 and miR-194 influenced the epithelial-mesenchymal transition (EMT) process of colorectal adenocarcinoma (CRA). METHODS: Totally 214 pairs of CRA and adjacent normal tissues were collected, and 5 human CRA cell lines (i.e. HCT116, HT-29, RKO SW280 and Lovo) were purchased...
October 2, 2018: Cellular Physiology and Biochemistry
Yan-Feng Huang, Wen-Bo Niu, Rong Hu, Ling-Jun Wang, Zeng-Yan Huang, Shi-Hao Ni, Ming-Qing Wang, Yi Yang, Yu-Sheng Huang, Wen-Jun Feng, Wei Xiao, Da-Jian Zhu, Shao-Xiang Xian, Lu Lu
Colorectal cancer stem cells (CSCs), characterized by self-renewal ability and high expression of proliferative genes, contribute to the chemoresistance of colorectal cancer (CRC). We aimed to identify the molecular mechanisms underlying CRC chemoresistance through comprehensive bioinformatics screenings and experimental confirmation of gene functions. We found that high expression of FGF1 intracellular binding protein (FIBP) was correlated with chemoresistance and poor prognosis in CRC patients. Therefore, the chemoresistant CRC cell line HCT116-CSC with high expression of the stem cell markers CD44 and CD133 was established for further phenotypic tests...
October 2, 2018: Oncogenesis
Takaki Akamine, Yosuke Morodomi, Yui Harada, Koji Teraishi, Tetsuzo Tagawa, Tatsuro Okamoto, Yoshihiko Maehara, Yoshikazu Yonemitsu
BACKGROUND/AIM: Alterations of microRNA expression in three-dimensional spheroids were examined to identify novel microRNAs that might be associated with tumorigenesis. MATERIALS AND METHODS: Using microRNA microarray analysis, we screened for microRNAs that were dramatically up-regulated inside three-dimensional spheroids in genetically-modified HCT116 human colon cancer cells expressing Copepoda Green Fluorescent Protein under hypoxia. RESULTS: miR-3148 was identified as a possible candidate onco-microRNA...
October 2018: Anticancer Research
Nalinee Kanth Kadiyala, Badal Kumar Mandal, Shivendu Ranjan, Nandita Dasgupta
The development of novel functionalized reduced graphene oxide nanocomposite materials keeping in mind environmental and health perspectives via green approaches is currently gaining enormous research interest in the field of nanoscience and nanotechnology. Herein, we report a bio-inspired green synthesis approach for gold nanoparticles decorated reduced graphene oxide nanocomposite in which Syzygium cumini seed extract (SCSE) is applied as natural reducing and stabilizing agent for the simultaneous reduction of chloroauric acid and graphene oxide (GO)...
December 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Jin-Woo Jeong, Cheol Park, Hee-Jae Cha, Su Hyun Hong, Shin-Hyung Park, Gi-Young Kim, Woo Jean Kim, Cheol Hong Kim, Kyoung Seob Song, Yung Hyun Choi
Prostaglandin E2 (PGE2), a major product of cyclooxygenase-2 (COX-2), plays an important role in the carcinogenesis of many solid tumors, including colorectal cancer. Because PGE2 functions by signaling through PGE2 receptors (EPs), which regulate tumor cell growth, invasion, and migration, there has been a growing amount of interest in the therapeutic potential of targeting EPs. In the present study, we investigated the role of EP4 on the effectiveness of cordycepin in inhibiting the migration and invasion of HCT116 human colorectal carcinoma cells...
October 1, 2018: BMB Reports
Yu-Wen Zhuang, Cun-En Wu, Jin-Yong Zhou, Zhi-Ming Zhao, Chun-Li Liu, Jun-Yi Shen, Hui Cai, Shen-Lin Liu
Adverse side effects of conventional chemotherapy, acquired resistance and fatal tumor metastasis of human colorectal cancer (CRC) are propelling the exploration for novel selective anticarcinogens. Solasodine is a main active component isolated from Solanum incanum L that exhibited a potent stemness and invasion inhibitory effect on human colorectal cancer HCT116 cells. Colony Spheroid formation assay showed that solasodine dose-dependently prohibited HCT116 cell stemness. CD133, CD44, Nanog, Oct-4 and Sox-2 were inhibited by solasodine to reverse stemness and similar mechanism was stimulated in vivo...
September 26, 2018: Biochemical and Biophysical Research Communications
Jing Ying Wang, Zhe Wang, Ming Yue Li, Zhihong Zhang, Chunliu Mi, Hong Xiang Zuo, Yue Xing, Yan-Ling Wu, Li-Hua Lian, Guang Hua Xu, Lian Xun Piao, Juan Ma, Xuejun Jin
Dictamnine (DTM) is a natural alkaloid isolated from the root of Dictamnus dasycarpus Turcz and has been shown to exhibit multiple biological functions, including anti-inflammatory, antifungal, anti-angiogenic and anticancer activity. However, the mechanisms by which dictamnine inhibits tumor growth are not fully understood. In this study, we investigated the effectiveness of dictamnine as a treatment for cancer and to identify the underlying mechanisms of its anticancer activity. Here, dictamnine showed the potent inhibitory activity against HIF-1α and Slug activation induced by hypoxia in various human cancer cell lines...
September 25, 2018: Chemico-biological Interactions
Lorena Veneziano, Viviana Barra, Danilo Cilluffo, Aldo Di Leonardo
The spindle assembly checkpoint (SAC) is a cellular surveillance mechanism that ensures the fidelity of chromosomes segregation. Reduced expression of some of its components weakens the SAC and induces chromosome instability and aneuploidy, which are both well-known hallmarks of cancer cells. Centromere protein-E (CENP-E) is a crucial component of the SAC and its function is to facilitate kinetochore microtubule attachment required to achieve and maintain chromosome alignment. The present study investigates the possible role of p14ARF as a controller of aneuploid cells proliferation...
September 27, 2018: Molecular Genetics and Genomics: MGG
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