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Juan Zhang, Dake Chu, Takuji Kawamura, Kiyohito Tanaka, Shuixiang He
Hypoxia leads to cancer progression and promotes the metastatic potential of cancer cells. Thereby, the aim of the present study was to investigate the detailed effects of gene associated with retinoid-interferon-induced mortality-19 (GRIM-19) in colorectal cancer (CRC) cell lines under hypoxia conditions and explore the potential molecular mechanisms. Here, we observed that GRIM-19 expression was downregulated in several CRC cell lines as well as in HCT116 and Caco-2 cells under a hypoxic microenvironment...
December 7, 2018: Journal of Cellular Physiology
Shang-Ze Li, Jian-Wei Ren, Jing Fei, Xiao-Dong Zhang, Run-Lei Du
Cordycepin, or 3'‑deoxyadenosine, is a derivative of the nucleoside adenosine. Initially extracted from the fungus Cordyceps militaris, cordycepin exhibits antitumor activity against certain cancer cell lines; however, the mechanism by which cordycepin counteracts colorectal cancer (CRC) remains poorly understood. The aim of the present study was to explore the underlying mechanisms of cordycepin against human CRC. To investigate the molecular mechanisms of cordycepin against colon cancer and in driving apoptosis, p53 and Bcl‑2‑like protein 4‑null (Bax‑/‑) colon cancer HCT116 cell lines were used...
December 3, 2018: Molecular Medicine Reports
Nanpeng Li, Daojiang Li, Yuheng Du, Chen Su, Chunxing Yang, Changwei Lin, Xiaorong Li, Gui Hu
Researchers hold the view that PLAGL2 is overexpressed in many malignancies and that it can promote tumor proliferation, migration, invasion and self‑renewal; however, there is no evidence revealing a relationship between PLAGL2 and colorectal cancer (CRC). In the present study, genes that are overexpressed in CRC were screened using the COSMIC database and GEPIA database and the expression of PLAGL2 in carcinoma tissues and pericarcinomatous tissues was detected by RT‑qPCR and western blot assays. A Cell Counting Kit‑8 assay, a cell cycle analysis experiment and a xenograft model were used to explore the influence of PLAGL2 on CRC after knocking down PLAGL2 expression in HCT116 and SW480 cells...
December 7, 2018: Oncology Reports
Praveen Pandey, Deepika Singh, Mohammad Hasanain, Raghib Ashraf, Mayank Maheshwari, Kuldeep Choyal, Akhilesh Singh, Dipak Datta, Brijesh Kumar, Jayanta Sarkar
Sphaeranthus indicus Linn. is commonly used in Indian traditional medicine for management of multiple pathological conditions. However, there are limited studies on anti-cancer activity of this plant and its underlying molecular mechanisms. Here, we isolated an active constituent, 7-hydroxyfrullanolide (7-HF), from the flowers of this plant which showed promising chemotherapeutic potential. The compound was more effective in inhibiting in vitro proliferation of colon cancers cells through G2/M phase arrest than other cancer cell lines that were used in the present study...
December 8, 2018: Carcinogenesis
Miri Lee, Hyunju Choi, Kyoung-Sook Kim, Dong-Hyun Kim, Cheorl-Ho Kim, Young-Choon Lee
Our recent report showed that curcumin, polyphenolic compound isolated from the herb Curcuma longa , upregulated the gene expression of human GD3 synthase (hST8Sia I) responsible for ganglioside GD3 synthesis with autophagy induction in human lung adenocarcinoma A549 cells. In this study, on the contrary to this finding, we demonstrated that curcumin downregulated the gene expression of human GM3 synthase (hST3Gal V) catalyzing ganglioside GM3 synthesis with autophagy induction in human colon carcinoma HCT116 cells...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Zeting Yuan, Yuxia Yuan, Lin Han, Yanyan Qiu, Xiaqin Huang, Feng Gao, Guohua Fan, Yixi Zhang, Xueyao Tang, Xue He, Ke Xu, Peihao Yin
Background: Multidrug resistance (MDR) is the major reason for the failure of chemotherapy in colon cancer. Bufalin (BU) is one of the most effective antitumor active constituents in Chansu. Our previous study found that BU can effectively reverse P-glycoprotein (P-gp)-mediated MDR in colon cancer. However, the clinical application of BU is limited due to its low solubility in water and high toxicity. In the present study, a multifunctional delivery system based on vitamin-E- succinate grafted chitosan oligosaccharide (VES-CSO) and cyclic (arginine-glycine-aspartic acid peptide) (RGD)-modified d-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) was prepared by emulsion solvent evaporation method for targeted delivery of BU to improve the efficacy of drug-resistant colon cancer therapy...
2018: International Journal of Nanomedicine
Zhenqiang Sun, Chunlin Ou, Jinbo Liu, Chen Chen, Quanbo Zhou, Shuaixi Yang, Guiyuan Li, Guixian Wang, Junmin Song, Zhen Li, Zhiyong Zhang, Weitang Yuan, Xiayu Li
Yes-associated protein 1 (YAP1) exerts significant effects in various malignancies. However, the oncogenic role of YAP1 remains controversial, and the mechanism by which YAP1 regulates non-coding RNAs is still largely unknown. The present study aimed to assess the effect of YAP1 on the malignant behaviors of colorectal carcinoma (CRC) and explore the underlying regulatory mechanism of the YAP1-MALAT1-miR-126-5p axis. YAP1 was highly expressed in CRC tissues as assessed by GSE20916 and its expression was negatively correlated with overall survival in 83 CRC cases...
December 10, 2018: Oncogene
Aistė Skeberdytė, Ieva Sarapinienė, Jan Aleksander-Krasko, Vaidotas Stankevičius, Kęstutis Sužiedėlis, Sonata Jarmalaitė
In the present study, we examined a hypothesis that dichloroacetate, a metabolic inhibitor, might efficiently potentiate the cytotoxic effect of salinomycin, an antibiotic ionophore, on two human colorectal cancer derived cell lines DLD-1 and HCT116. First, we performed a series of dose response experiments in the 2D cell culture by applying mono- and combination therapy and by using the Chou-Talalay method found that salinomycin in combination with dichloroacetate acted synergistically in both cell lines. Secondly, in order to recapitulate the in vivo tumor architecture, we tested various doses of these compounds, alone and in combination, in the 3D multicellular spheroid culture...
December 10, 2018: Scientific Reports
Kun Wang, Tengyan Li, Chang Xu, Yuhan Ding, Wenjing Li, Lei Ding
The dysregulation of the tight junctions (TJs) protein claudin-7 is closely related to the development and metastasis of colorectal cancer (CRC). The aim of this study was to investigate the expression of claudin-7 and characterize the relationship between claudin-7 expression and epithelial-mesenchymal transition (EMT) in CRC. In this study, the expression of claudin-7, E-cadherin, vimentin and snail-1 was detected by immunohistochemistry (IHC) in a set of 80 CRC specimens comprising 20 specimens each of well-differentiated, moderately differentiated, poorly differentiated and liver metastases tissues...
December 6, 2018: Biochemical and Biophysical Research Communications
Riham F George, Nermin Samir, Iriny M Ayoub, ElSayed M Shalaby, Nicola Demitri, Michael Wink
AIM: Colon cancer is the third leading cause of death worldwide; therefore, there is a need for an effective therapy with lower side effects. METHODS: A series of 8-alkyl-2,4-bisbenzylidene-3-nortropinones 3 & 14-39 was prepared via Claisen-Schmidt condensation of 8-alkyl-3-nortropinones 11-13 with different aromatic aldehydes. The target compounds were screened for their antiproliferative activity. RESULTS: Most of the prepared compounds showed promising antiproliferative activity against many of 60 National Cancer Institute cell lines at 10 μM...
December 10, 2018: Future Medicinal Chemistry
Sun-Hyung Ha, Kyung-Min Kwon, Jun-Young Park, Fukushi Abekura, Young-Choon Lee, Tae-Wook Chung, Ki-Tae Ha, Hyeun Wook Chang, Seung-Hak Cho, Jong-Suk Kim, Cheorl-Ho Kim
A water-soluble saponin, Esculentoside H (EsH), 3-O-(O-β-d-glucopyranosyl-(1→4)-β-d-xylopyranosyl)-28-β-d-glucopyranosylphytolaccagenin has been isolated and purified from the root extract of perennial plant Phytolacca esculenta. EsH is known to be an anticancer compound, having a capacity for TNF-α release. However, the effects of EsH on migration and growth in tumor cells have not yet been reported. In the current study, the suppressive effects of EsH on phorbol 12-myristate 13-acetate (PMA)-induced cell migration were examined in murine colon cancer CT26 cells and human colon cancer HCT116 cells...
December 7, 2018: Journal of Cellular Biochemistry
Xinbing Sui, Ruonan Zhang, Shuiping Liu, Ting Duan, Lijuan Zhai, Mingming Zhang, Xuemeng Han, Yu Xiang, Xingxing Huang, Haoming Lin, Tian Xie
Ferroptosis is an iron-dependent, oxidative cell death, and is characterized by iron-dependent accumulation of reactive oxygen species (ROS) within the cell. It has been implicated in various human diseases, including cancer. Recently, ferroptosis, as a non-apoptotic form of cell death, is emerging in specific cancer types; however, its relevance in colorectal cancer (CRC) is unexplored and remains unclear. Here, we showed that ferroptosis inducer RSL3 initiated cell death and ROS accumulation in HCT116, LoVo, and HT29 CRC cells over a 24 h time course...
2018: Frontiers in Pharmacology
Danhua Zhang, Xinguang Qiu, Jianhua Li, Shouhua Zheng, Liwen Li, Hongchao Zhao
Tumor-associated macrophages (TAMs) were reported to be involved in colorectal cancer (CRC) progression. However, its biological role and underlying mechanism in CRC remained to be elucidated. In this study, the expressions of the macrophage marker CD68 and transforming growth factor β1 (TGF-β1) in CRC tumor tissues and adjacent tissues were detected by immunohistochemistry. The expression levels of miR-34a, TGF-β1 and vascular endothelial growth factor (VEGF) in CRC tumor tissues and peripheral blood macrophages were measured by quantitative real-time PCR (qRT-PCR) and western blot...
December 7, 2018: Cell Cycle
Chunlai Feng, Xianqin Yuan, Kexin Chu, Haisheng Zhang, Wei Ji, Mengjie Rui
Fisetin is a natural flavonoid with promising antitumor activity, whereas its clinical application is limited by its hydrophobic property. In this study, we aimed to load fisetin into poly(lactic acid) (PLA) nanoparticles to increase fisetin's solubility and therapeutic efficacy. Based on spontaneous emulsification solvent diffusion (SESD) method, the formulation of PLA nanoparticles was optimized by two successive experimental designs. One-factor-at-a-time variation experiments were first applied to investigate the effects of four process variables on three responses, including drug encapsulation efficiency, average particles size and cumulative drug release ratio, followed by determining the possible ranges of these variables...
December 3, 2018: International Journal of Biological Macromolecules
Saloni Kakkar, Sanjiv Kumar, Siong Meng Lim, Kalavathy Ramasamy, Vasudevan Mani, Syed Adnan Ali Shah, Balasubramanian Narasimhan
BACKGROUND: In view of wide range of biological activities of oxazole, a new series of oxazole analogues was synthesized and its chemical structures were confirmed by spectral data (Proton/Carbon-NMR, IR, MS etc.). The synthesized oxazole derivatives were screened for their antimicrobial and antiproliferative activities. RESULTS AND DISCUSSION: The antimicrobial activity was performed against selected fungal and bacterial strains using tube dilution method. The antiproliferative potential was evaluated against human colorectal carcinoma (HCT116) and oestrogen- positive human breast carcinoma (MCF7) cancer cell lines using Sulforhodamine B assay and, results were compared to standard drugs, 5-fluorouracil and tamoxifen, respectively...
December 4, 2018: Chemistry Central Journal
Kexu Chen, Hanlin Liang, Jiewen Peng, Yanfang Zheng
OBJECTIVE: To investigate the expressions of secreted frizzled-related protein 4 (SFRP4) in stage Ⅱ DNA mismatch repair-deficient (dMMR) and mismatch repair- proficient (pMMR) colorectal cancers and explore their clinical significance. METHODS: We collected fresh stage Ⅱ colon cancer tissues with different MMR status detected by immunohistochemistry (IHC). The differentially expressed mRNAs between dMMR and pMMR tumors were identified by Affymetrix Human oeLncRNA gene chip, and the expression of SFRP4 in these cancer tissues and in colorectal cancer cell lines were detected using Western blotting and real- time quantitative PCR...
November 30, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Gokhan Zengin, Marcello Locatelli, Claudio Ferrante, Luigi Menghini, Giustino Orlando, Luigi Brunetti, Lucia Recinella, Annalisa Chiavaroli, Sheila Leone, Lidia Leporini, Muhammad Zakariyyah Aumeeruddy, Mohamad Fawzi Mahomoodally
This study explored the efficacy of the methanolic extract of three Asphodeline species (A. damascena subsp. rugosa, A. tenuior subsp. tenuiflora var. tenuiflora, and A. cilicica) to protect against hydrogen peroxide (H2 O2 )-induced lactate dehydrogenase (LDH) activity in HCT116 cells, and also any protective effects against lipopolysaccharides (LPS)-induced nitrite levels, prostaglandin E2 (PGE2) and 8-iso-prostaglandin F2α (8-iso-PGF2α) levels, 5HIAA/5-HT ratio, tumor necrosis factor (TNF)-α and interleukin (IL)-6 gene expression in rat colon specimens...
December 5, 2018: International Journal of Environmental Health Research
Yang-Ping Ding, Zi-Li Gao, Bing-Can Chen, Kanyasiri Rakariyatham, Hua-Yi Suo, Hua-Rong Tong, Hang Xiao
Backgroud: (-)-Epigallocatechin-3-gallate (EGCG), the major component of green tea, is well documented to induce apoptosis and cell cycle arrest in cancer by targeting multiple signal transduction pathways. However, EGCG is extremely unstable in general culture conditions and rapidly degraded. So, to what extent EGCG or which degradation products of EGCG play a role in anti-tumor is still unknown. In this study, we evaluated the effect of different treatments of EGCG on HCT116 cells. DESIGN: MTT assay was applied to evaluated the inhibitory effect of different treatments of EGCG on HCT116 cells...
December 4, 2018: Nutrition and Cancer
Zhong-Zhu Chen, Shi-Qiang Li, Ya-Jun Zhang, Dian-Yong Tang, Jiang-Ping Meng, Jie Lei, Hong-Yu Li, Zhi-Gang Xu
A novel three-component cascade reaction was discovered and developed to synthesize pyridodiindoles with the assistance of microwave irradiation. A collection of pyridodiindoles was prepared by means of the mild reaction and simple operation procedure, which could be applicable to a broad scope of functional aldehydes. Screening demonstrated that compound 5g exhibited a good potency in HCT116 cell lines, and this work validated the feasibility of this novel reaction for generating promising bioactive compounds...
December 4, 2018: Organic Letters
Salem Majdop, Yehuda Skornick, Shmuel Avital, Liron Berkovich
Cancer-associated fibroblasts of the stroma play a major role in tumor promoting processes. In this study we evaluated the significance of Phospholipase D (PLD) enzyme activity in promoting human colon cancer malignant potency when interacting with proximal colonic fibroblasts. Human colon cancer cell lines SW480 and HCT116, and colonic fibroblasts CCD-18Co were used as an in vitro model. PLD's activity was measured in resting cancer cells and after culturing with fibroblasts and cancer-associated fibroblasts (CAFs) conditioned medium...
November 30, 2018: Cellular and Molecular Biology
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