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https://www.readbyqxmd.com/read/29775727/isolation-structure-elucidation-and-antibacterial-activity-of-methyl-4-8-dimethylundecanate-from-the-marine-actinobacterium-streptomyces-albogriseolus-ecr64
#1
Durairaj Thirumurugan, Ramasamy Vijayakumar, Chithravel Vadivalagan, Pushparaj Karthika, Md Khurshid Alam Khan
Around 120 actinobacterial colonies were isolated from various regions of marine East coast region of Tamil Nadu, India. Among them, 33 were morphologically distinct and they were preliminarily screened for their antibacterial activity against Pseudomonas fluorescens, Vibrio cholerae, V. parahaemolyticus, V. alginolyticus, and Aeromonas hydrophila by cross-streak plate technique. Among the isolated, the isolate ECR64 exhibited maximum zone of inhibition against fish pathogenic bacteria. The crude bioactive compounds were extracted from the isolate ECR64 using different organic solvents which exhibited maximum antibacterial activity...
May 15, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29775426/impact-of-a-native-streptomyces-flavovirens-from-mushroom-compost-on-green-mold-control-and-yield-of-agaricus-bisporus
#2
Ljiljana Šantrić, Ivana Potočnik, Ljiljana Radivojević, Jelena Gajić Umiljendić, Emil Rekanović, Bojan Duduk, Svetlana Milijašević-Marčić
Thirty-five actinobacterial isolates, obtained from button mushroom (Agaricus bisporus) substrates (i.e., compost in different phases of composting, black peat or casing layer) in Serbia in 2014-2016 were tested in vitro against the causal agents of green mold in cultivated mushroom. Out of six most promising isolates, A06 induced 42.4% in vitro growth inhibition of Trichoderma harzianum T54, and 27.6% inhibition of T. aggressivum f. europaeum T77. The novel strain A06 was identified as Streptomyces flavovirens based on macroscopic and cultural characteristics and 16S rDNA sequence and used in mushroom growing room experiments...
May 18, 2018: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/29772796/precursor-directed-generation-of-indolocarbazoles-with-topoisomerase-ii%C3%AE-inhibitory-activity
#3
Cong Wang, Adeep Monger, Liping Wang, Peng Fu, Pawinee Piyachaturawat, Arthit Chairoungdua, Weiming Zhu
One new indolocarbazole, 3-hydroxy-K252d ( 3 ), together with the recently reported 3-hydroxyholyrine A ( 1 ) and 3'- N -acetyl-3-hydroxyholyrine A ( 2 ), were obtained by feeding a culture of the marine-derived Streptomyces strain OUCMDZ-3118 with 5-hydroxy-l-tryptophan. Their structures were elucidated on the basis of spectroscopic analysis. Compound 1 potently induced apoptosis of gastric cancer cells by inhibiting topoisomerase IIα enzyme activity and reducing the expression of antiapoptosis protein level...
May 17, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29770710/convergent-synthesis-of-carpatamide-a-cytotoxic-arylamine-derivative-from-marine-derived-streptomyces-sp
#4
Rohani Prasad Burman, Shantanu Gupta, Jyoti Bhatti, Krishan Verma, Devendra Rajak, Manjinder Singh Gill
First total synthesis of carpatamide-A 7a, cytotoxic arylamine derivative isolated from marine derived Streptomyces sp., was achieved in twelve steps with overall yield of 24% with seven longest linear steps. In the penultimate step, dienoic acid 13 and an amino-phenylpropionic acid methyl ester core 21 were coupled to synthesize methylated derivativative of carpatamide-A 22 followed by demethylation of the intermediate with BBr3 to accomplish carpatamide-A 7a. Both precursors 13 and 21 were synthesized from readily available starting materials i...
May 17, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29767975/cytotoxic-anthracycline-metabolites-from-a-recombinant-streptomyces
#5
Chun Gui, Jie Yuan, Xuhua Mo, Hongbo Huang, Shanwen Zhang, Yu-Cheng Gu, Jianhua Ju
The C7 (C9 or C10)- O-l-rhodosamine-bearing anthracycline antibiotic cytorhodins and their biosynthetic intermediates were recently isolated from Streptomyces sp. SCSIO 1666. Cosmid p17C4 from the Streptomyces lydicus genomic library, which harbors both the biosynthetic genes for l-rhodinose (or 2-deoxy-l-fucose) and its glycosyltransferase (encoded by slgG), was introduced into SCSIO 1666 to yield the recombinant strain Streptomyces sp. SCSIO 1666/17C4. Chemical investigations of this strain's secondary metabolic potential revealed the production of different anthracyclines featuring C7- O-l-rhodinose (or 2-deoxy-l-fucose) instead of the typically observed l-rhodosamine...
May 16, 2018: Journal of Natural Products
https://www.readbyqxmd.com/read/29767273/correction-to-expanding-tryptophan-containing-cyclodipeptide-synthase-spectrum-by-identification-of-nine-members-from-streptomyces-strains
#6
Jing Liu, Huili Yu, Shu-Ming Li
The original version of this article contained mistake. After careful re-examination of the LC-MS data, the products of CDPSs "WP_031028810" and "BAU83478" should be cFL instead of cPY. We apologize for any inconvenience that this may have caused.
May 16, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29767085/nitrogen-fractionation-of-certain-conventional-and-lesser-known-by-products-for-ruminants
#7
M S Mahesh, Sudarshan S Thakur, Rohit Kumar, Tariq A Malik, Rajkumar Gami
Dietary proteins for ruminants are fractionated according to solubility, degradability and digestibility. In the present experiment, 11 vegetable protein meals and cakes used in ruminant nutrition were included with a main focus on determining various nitrogen (N) fractions in vitro . Total N (N × 6.25) content varied from 22.98% (mahua cake) to 65.16% (maize gluten meal), respectively. Guar meal korma contained the lowest and rice gluten meal had the highest acid detergent insoluble nitrogen (ADIN; N × 6...
June 2017: Animal nutrition
https://www.readbyqxmd.com/read/29766001/production-of-recombinant-streptavidin-and-optimization-of-refolding-conditions-for-recovery-of-biological-activity
#8
Raoufe Modanloo Jouybari, Abdorrahim Sadeghi, Behzad Khansarinejad, Shabnam Sadoogh Abbasian, Hamid Abtahi
Background: Streptavidin is a protein produced by Streptomyces avidinii with strong biotin-binding ability. The non-covalent, yet strong bond between these two molecules has made it a preferable option in biological detection systems. Due to its extensive use, considerable attention is focused on streptavidin production by recombinant methods. Methods: In this study, streptavidin was expressed in Escherichia coli (E. coli) BL21 (DE3) pLysS cells and purified by affinity chromatography...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29757948/streptomyces-differentiation-in-liquid-cultures-as-a-trigger-of-secondary-metabolism
#9
REVIEW
Ángel Manteca, Paula Yagüe
Streptomyces is a diverse group of gram-positive microorganisms characterised by a complex developmental cycle. Streptomycetes produce a number of antibiotics and other bioactive compounds used in the clinic. Most screening campaigns looking for new bioactive molecules from actinomycetes have been performed empirically, e.g., without considering whether the bacteria are growing under the best developmental conditions for secondary metabolite production. These screening campaigns were extremely productive and discovered a number of new bioactive compounds during the so-called "golden age of antibiotics" (until the 1980s)...
May 14, 2018: Antibiotics
https://www.readbyqxmd.com/read/29755552/efficacy-of-alpha-glucosidase-inhibitor-from-marine-actinobacterium-in-the-control-of-postprandial-hyperglycaemia-in-streptozotocin-stz-induced-diabetic-male-albino-wister-rats
#10
S R Sathish Kumar, K V Bhaskara Rao
The current study was carried out to evaluate the in-vitro and in-vivo efficiency of alpha glucosidase inhibitor of marine actinobacteria in the control of postprandial hyperglycaemia. Soil samples were collected from salterns, coastal area in Kothapatnam, Ongole, Andhra Pradesh, India. Among the actinobacterial isolates tested for yeastα-glucosidase inhibitory activity, only three isolates showed prominent inhibition. The patient isolate was selected and identified as Streptomyces coelicoflavus SRBVIT13 using 16S r-RNA gene sequencing...
2018: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/29753326/biosynthesis-of-angelyl-coa-in-saccharomyces-cerevisiae
#11
Roberta Callari, David Fischer, Harald Heider, Nora Weber
BACKGROUND: The angelic acid moiety represents an essential modification in many biologically active products. These products are commonly known as angelates and several studies have demonstrated their therapeutic benefits, including anti-inflammatory and anti-cancer effects. However, their availability for use in the development of therapeutics is limited due to poor extraction yields. Chemical synthesis has been achieved but its complexity prevents application, therefore microbial production may offer a promising alternative...
May 12, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29752478/bafilomycin-c1-induces-g0-g1-cell-cycle-arrest-and-mitochondrial-mediated-apoptosis-in-human-hepatocellular-cancer-smmc7721-cells
#12
Xiaoxiao Gao, Li Han, Nan Ding, Yu Mu, Peipei Guan, Caijuan Hu, Xueshi Huang
Bafilomycin C1, which was isolated from Streptomyces albolongus in our previous work, exhibited strong cytotoxicity against several cancer cell lines. This study aimed to evaluate its antitumor effect on human hepatocellular cancer SMMC7721 cells and the underlying mechanism in vitro and in vivo. MTT assay revealed that bafilomycin C1 retarded SMMC7721 cell growth and proliferation. Western blot and real-time qPCR analysis revealed that bafilomycin C1 caused partial G0/G1 phase cell-cycle arrest, downregulated the expression of cyclin D3, cyclin E1, CDK2, CDK4, and CDK6 and upregulated the expression of p21...
May 11, 2018: Journal of Antibiotics
https://www.readbyqxmd.com/read/29752077/tissue-adhesive-rapid-forming-and-sprayable-ecm-hydrogel-via-recombinant-tyrosinase-crosslinking
#13
Su-Hwan Kim, Sang-Hyuk Lee, Ju-Eun Lee, Sung Jun Park, Kyungmin Kim, In Seon Kim, Yoon-Sik Lee, Nathaniel S Hwang, Byung-Gee Kim
We report on a tissue adhesive hydrogel based on novel recombinant tyrosinase mediated crosslinking. The adhesive hydrogels were fabricated by the site-directed coupling of tyramine-conjugated hyaluronic acid (HA_t, 1% w/v) and gelatin (3% w/v) (HG_gel) with novel tyrosinase derived from Streptomyces avermitilis (SA_Ty). The enzyme-based crosslinking by SA_Ty was fast, with less than 50 s for complete gelation, and the SA_Ty based crosslinking enhanced the physical properties and adhesive strength of the hydrogel significantly with the native tissue samples...
May 3, 2018: Biomaterials
https://www.readbyqxmd.com/read/29749536/biib021-an-hsp90-inhibitor-a-promising-therapeutic-strategy-for-blood-malignancies-review
#14
Wei He, Huixian Hu
Heat shock proteins (HSPs) are molecular chaperones that are consistently increased to help cells survive under conditions of stress. As a member of the Hsps, Hsp90 is involved in protein post‑translational maturation and disposition. This protein is ubiquitously expressed in normal cells. However, in cancer cells and particularly in hematological malignancies, Hsp90 is unexpectedly abundant to maintain levels of proteins vital for cancer pathology. Hsp90 inhibitors can target the ATP domain of Hsp90 and prohibit its exchange of ADP for ATP, leading to the degradation of client proteins and disruption of signaling cascades...
May 8, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29748634/gene-by-environment-interactions-that-disrupt-mitochondrial-homeostasis-cause-neurodegeneration-in-c-elegans-parkinson-s-models
#15
Hanna Kim, Rylee J Perentis, Guy A Caldwell, Kim A Caldwell
Parkinson's disease (PD) is a complex multifactorial disorder where environmental factors interact with genetic susceptibility. Accumulating evidence suggests that mitochondria have a central role in the progression of neurodegeneration in sporadic and/or genetic forms of PD. We previously reported that exposure to a secondary metabolite from the soil bacterium, Streptomyces venezuelae, results in age- and dose-dependent dopaminergic (DA) neurodegeneration in Caenorhabditis elegans and human SH-SY5Y neurons...
May 10, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29746664/translational-control-plays-an-important-role-in-the-adaptive-heat-shock-response-of-streptomyces-coelicolor
#16
Giselda Bucca, Radhika Pothi, Andrew Hesketh, Carla Möller-Levet, David A Hodgson, Emma E Laing, Graham R Stewart, Colin P Smith
Stress-induced adaptations require multiple levels of regulation in all organisms to repair cellular damage. In the present study we evaluated the genome-wide transcriptional and translational changes following heat stress exposure in the soil-dwelling model actinomycete bacterium, Streptomyces coelicolor. The combined analysis revealed an unprecedented level of translational control of gene expression, deduced through polysome profiling, in addition to transcriptional changes. Our data show little correlation between the transcriptome and 'translatome'; while an obvious downward trend in genome wide transcription was observed, polysome associated transcripts following heat-shock showed an opposite upward trend...
May 9, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29743540/sporulation-specific-cell-division-defects-in-ylme-mutants-of-streptomyces-coelicolor-are-rescued-by-additional-deletion-of-ylmd
#17
Le Zhang, Joost Willemse, Paul A Hoskisson, Gilles P van Wezel
Cell division during the reproductive phase of the Streptomyces life-cycle requires tight coordination between synchronous formation of multiple septa and DNA segregation. One remarkable difference with most other bacterial systems is that cell division in Streptomyces is positively controlled by the recruitment of FtsZ by SsgB. Here we show that deletion of ylmD (SCO2081) or ylmE (SCO2080), which lie in operon with ftsZ in the dcw cluster of actinomycetes, has major consequences for sporulation-specific cell division in Streptomyces coelicolor...
May 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29740520/the-%C3%AE-bt1-large-serine-recombinase-catalyzes-dna-integration-at-pseudo-attb-sites-in-the-genus-nocardia
#18
Marion Herisse, Jessica L Porter, Romain Guerillot, Takehiro Tomita, Anders Goncalves Da Silva, Torsten Seemann, Benjamin P Howden, Timothy P Stinear, Sacha J Pidot
Plasmid vectors based on bacteriophage integrases are important tools in molecular microbiology for the introduction of foreign DNA, especially into bacterial species where other systems for genetic manipulation are limited. Site specific integrases catalyze recombination between phage and bacterial attachment sites ( attP and attB , respectively) and the best studied integrases in the actinomycetes are the serine integrases from the Streptomyces bacteriophages ΦC31 and ΦBT1. As this reaction is unidirectional and highly stable, vectors containing phage integrase systems have been used in a number of genetic engineering applications...
2018: PeerJ
https://www.readbyqxmd.com/read/29740412/atmospheric-precipitations-hailstone-and-rainwater-as-a-novel-source-of-streptomyces-producing-bioactive-natural-products
#19
Aida Sarmiento-Vizcaíno, Julia Espadas, Jesús Martín, Alfredo F Braña, Fernando Reyes, Luis A García, Gloria Blanco
A cultivation-dependent approach revealed that highly diverse populations of Streptomyces were present in atmospheric precipitations from a hailstorm event sampled in February 2016 in the Cantabrian Sea coast, North of Spain. A total of 29 bioactive Streptomyces strains isolated from small samples of hailstone and rainwater, collected from this hailstorm event, were studied here. Taxonomic identification by 16S rRNA sequencing revealed more than 20 different Streptomyces species, with their closest homologs displaying mainly oceanic but also terrestrial origins...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29739601/cloning-characterization-and-substrate-degradation-mode-of-a-novel-chitinase-from-streptomyces-albolongus-atcc-27414
#20
Li Gao, Jianan Sun, Francesco Secundo, Xin Gao, Changhu Xue, Xiangzhao Mao
A novel chitinase gene was cloned from Streptomyces albolongus ATCC 27414, and expressed successfully in Escherichia coli BL21. The recombinant enzyme (SaChiA4) belongs to glycoside hydrolases (GH) family 18 and consists of a catalytic domain and a chitin binding domain (CBD) in its C-terminus. SaChiA4 was purified homogeneously (specific activity of 66.2 U/mg with colloidal chitin as substrate), and showed a molecular mass of approximately 47 kDa. SaChiA4 showed its optimal activity at pH 5.0 and 55 °C and exhibited remarkable pH and temperature stability...
September 30, 2018: Food Chemistry
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