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Chaturbhuj Agrawal, Vineet Talwar, Rajeev Saini, Pradeep Babu
Chemotherapy-induced skin rashes are common toxicities encountered which require careful assessment and evaluation as rashes could be a manifestation of primary malignancy itself and a variety of drugs used in combination further complicate the clinical scenario. Bleomycin is an anticancer antibiotic derived from Streptomyces verticillus and has been commonly used in the treatment of Hodgkin's disease, germ cell tumors and for pleurodesis. There are various dermatological adverse effects of bleomycin which have been previously reported in literature including skin peeling, hyperkeratosis, nail bed changes, Raynaud's phenomenon, and palmoplantar desquamation...
October 2017: Indian Journal of Medical and Paediatric Oncology
Siamak Semsary, Ivana Crnovčić, Ronja Driller, Joachim Vater, Bernhard Loll, Ullrich Keller
X-type actinomycins (Acms) contain 4-hydroxyproline (Acm X0) or 4-oxoproline (Acm X2) in their ß-pentapeptide lactone rings whereas their α-ring contains proline. We demonstrate that these Acms are formed through asymmetric condensation of Acm half molecules (Acm halves) containing proline with 4-hydroxyproline- or 4-oxo-proline-containing Acm halves. In turn we show - using an artificial Acm half analogue (PPL 1) with proline in its peptide chain - its conversion into the 4-hydroxyproline and 4-oxoproline-containing Acm halves, PPL 0 and PPL 2, in mycelial suspensions of Streptomyces antibioticus...
January 12, 2018: Chembiochem: a European Journal of Chemical Biology
Tingting Cao, Yibo Shen, Junwei Zhao, Chongxi Liu, Xueli Zhao, Liying Jin, Yanjie Li, Xiangjing Wang, Wensheng Xiang
A novel actinobacterium, designated strain NEAU-QY24T, was isolated from the rhizosphere of corn (Zea mays L.). A polyphasic approach was employed to determine the taxonomic status of strain NEAU-QY24T. The isolate was found to have chemical and morphological properties of the genus Streptomyces, with high 16S rRNA gene sequence similarity to Streptomyces lanatus JCM 4332T (98.3%) and clustered phylogenetically with Streptomyces lannensis JCM 16578T (98.2%). The cell wall was found to contain meso-diaminopimelic acid and the whole cell sugars were identified as glucose and ribose...
January 11, 2018: Antonie Van Leeuwenhoek
Lee E Hughes, Christopher D Shaffer, Vassie C Ware, Issac Aguayo, Rahat M Aziz, Swapan Bhuiyan, Isaac S Bindert, Canyon K Calovich-Benne, Jordyn Chapman, Richard Donegan-Quick, Amal Farooq, Cynthia Garcia, Lee H Graham, Baylee Y Green, Margaret A Kenna, Emily R Kneeream, Christian E Laing, Catherine M Mageeney, Sandra N Meridew, Abigail R Mikolon, Ryann E Morgan, Subhayu Nayek, Idowu D Olugbade, Kim C Pike, Lauren E Schlegel, Taylor C Shishido, Tara Suresh, Nikita Suri, Kathleen Weston Hafer, Rebecca A Garlena, Daniel A Russell, Steven G Cresawn, Welkin H Pope, Deborah Jacobs-Sera, Graham F Hatfull
Cluster BE1 Streptomyces bacteriophages belong to the Siphoviridae, with genome sizes over 130 kbp, and they contain direct terminal repeats of approximately 11 kbp. Eight newly isolated closely related cluster BE1 phages contain 43 to 48 tRNAs, one transfer-messenger RNA (tmRNA), and 216 to 236 predicted open reading frames (ORFs), but few of their genes are shared with other phages, including those infecting Streptomyces species.
January 11, 2018: Genome Announcements
Dian Anggraini Suroto, Shigeru Kitani, Masayoshi Arai, Haruo Ikeda, Takuya Nihira
Phthoxazolin A, an oxazole-containing polyketide, has a broad spectrum of anti-oomycete activity and herbicidal activity. We recently identified phthoxazolin A as a cryptic metabolite of Streptomyces avermitilis that produces the important anthelmintic agent avermectin. Even though genome data of S. avermitilis is publicly available, no plausible biosynthetic gene cluster for phthoxazolin A is apparent in the sequence data. Here, we identified and characterized the phthoxazolin A (ptx) biosynthetic gene cluster through genome sequencing, comparative genomic analysis, and gene disruption...
2018: PloS One
Yong Zhou, Li-Rong Han, Hong-Wei He, Bu Sang, Dai-Lin Yu, Jun-Tao Feng, Xing Zhang
The effects of temperature, agitation and aeration on glycoprotein GP-1 production by Streptomyces kanasenisi ZX01 in bench-scale fermentors were systematically investigated. The maximum final GP-1 production was achieved at an agitation speed of 200 rpm, aeration rate of 2.0 vvm and temperature of 30 °C. By using a dynamic gassing out method, the effects of agitation and aeration on volumetric oxygen transfer coefficient (kLa) were also studied. The values of volumetric oxygen transfer coefficient in the logarithmic phase increased with increase of agitation speed (from 14...
January 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Lilya Horbal, Theresa Siegl, Andriy Luzhetskyy
The design and engineering of secondary metabolite gene clusters that are characterized by complicated genetic organization, require the development of collections of well-characterized genetic control elements that can be reused reliably. Although a few intrinsic terminators and RBSs are used routinely, their translation and termination efficiencies have not been systematically studied in Actinobacteria. Here, we analyzed the influence of the regions surrounding RBSs on gene expression in these bacteria. We demonstrated that inappropriate RBSs can reduce the expression efficiency of a gene to zero...
January 11, 2018: Scientific Reports
Yong Zhou, Xin Zhou, Dai-Lin Yu, Bu Sang, Jun-Tao Feng, Li-Rong Han, Xing Zhang
GP-1 is a novel glycoprotein produced by Streptomyces kanasenisi ZX01 that was isolated from soil near Kanas Lake with significant bioactivity against tobacco mosaic virus. However, extremely low fermentation production has largely hindered further research and market applications on glycoprotein GP-1. In this study, response surface methodology was used to optimize fermentation conditions in a shake flask for higher glycoprotein GP-1 production. When the optimized fermentation conditions were inoculum volume of 6%, initial pH of 6...
January 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Dahae Lee, Ki Sung Kang, Hae-Jeung Lee, Ki Hyun Kim
Platinum-based anticancer drug therapies can cause renal damage and apoptotic kidney cell damage. The development of reno- and kidney-protective molecules is therefore urgently required. To address this challenge, we explored secondary metabolites of termite-associated Streptomyces sp. RB1 isolated from the cuticle of the South African termite, Macrotermes natalensis for their renoprotective ability using bioassay-guided fractionation and LLC-PK1 cells. Chemical investigation of the MeOH extract of Streptomyces sp...
January 7, 2018: International Journal of Molecular Sciences
Yucheng Zhang, Guangde Jiang, Yousong Ding, Rosemary Loria
With few exceptions, thaxtomin A (ThxA), a nitrated diketopiperazine, is the pathogenicity determinant for plant pathogenic Streptomyces species. In Streptomyces scabiei (syn. S. scabies), the ThxA biosynthetic cluster is located within a 177 Kb mobile pathogenicity island, called the toxicogenic region (TR). In S. turgidiscabies, the ThxA biosynthetic cluster is located within a 674 Kb pathogenicity island (PAIst). Emergence of new plant pathogens occurs in this genus, but not frequently. This raises the question of whether mobilization of these pathogenicity regions, through mating, is widespread and whether TR and PAIst can confer plant pathogenicity...
January 9, 2018: Molecular Plant Pathology
Andrew William Robertson, Jeanna M MacLeod, Logan W MacIntyre, Stephanie M Forget, Steven R Hall, Leah G Bennett, Hebelin Correa, Russell G Kerr, Kerry B Goralski, David L Jakeman
Polyketide synthase (PKS) derived natural products are biosynthesized by head-to-tail addition of acetate and malonate extender units resulting in linear extended-polyketide chains. Despite the well documented structural diversity associated with PKS derived natural products, C-C chain branching deviating from the usual linear pattern is relatively rare. Herein, type-II PKS angucyclic natural products containing a hemiaminal functionality were identified and proposed as the parent of a series of C-C branched analogues...
January 9, 2018: Journal of Organic Chemistry
Mengxuan Chen, Weiyun Chai, Tengfei Song, Mingzhu Ma, Xiao-Yuan Lian, Zhizhen Zhang
Marine natural products are important resources for discovering novel anticancer drugs. In this study, an extract prepared from the culture of a sea anemone-derived actinomycete Streptomyces sp. ZZ406 in soluble starch and casein-related liquid medium was found to have activity in inhibiting the proliferation of glioma cells and reducing the production of lactate in glioma cells. Chemical investigation of this active crude extract resulted in the isolation of four new compounds and seven known ones. Structures of the new compounds were determined by a combination of extensive NMR analyses, HRESIMS and MS-MS data, electronic circular dichroism calculation, chemical degradation, and Marfey's method...
January 8, 2018: Scientific Reports
Sébastien Rigali, Sinaeda Anderssen, Aymeric Naômé, Gilles P van Wezel
The World Health Organization (WHO) describes antibiotic resistance as "one of the biggest threats to global health, food security, and development today", as the number of multi- and pan-resistant bacteria is rising dangerously. Acquired resistance phenomena also impair antifungals, antivirals, anti-cancer drug therapy, while herbicide resistance in weeds threatens the crop industry. On the positive side, it is likely that the chemical space of natural products goes far beyond what has currently been discovered...
January 5, 2018: Biochemical Pharmacology
Yuanyuan Hu, Mian Wang, Chunyan Wu, Yi Tan, Jiao Li, Xiaomeng Hao, Yanbo Duan, Yan Guan, Xiaoya Shang, Yiguang Wang, Chunling Xiao, Maoluo Gan
Analysis of the whole genome sequence of Streptomyces sp. IMB7-145 revealed the presence of seven type I polyketide synthase biosynthetic gene clusters, one of which was highly homologous to the biosynthetic gene cluster of azalomycin F. Detailed bioinformatic analysis of the modular organization of the PKS gene suggested that this gene is responsible for niphimycin biosynthesis. Guided by genomic analysis, a large-scale cultivation ultimately led to the discovery and characterization of four new niphimycin congeners, namely, niphimycins C-E (1-3) and 17-O-methylniphimycin (4)...
January 8, 2018: Journal of Natural Products
Mohammad Hadi Sekhavati, Sayed Morteza Hosseini, Mojtaba Tahmoorespur, Kamran Ghaedi, Farnoosh Jafarpour, Mehdi Hajian, Kyanoosh Dormiani, Mohammad Hossain Nasr-Esfahani
OBJECTIVES: The Streptomyces phage phiC31 integrase offers a sequence-specific method of transgenesis with a robust long-term gene expression. PhiC31 has been successfully developed in a variety of tissues and organs for purpose of in vivo gene therapy. The objective of the present experiment was to evaluate PhiC31-based site-specific transgenesis system for production of transgenic bovine embryos by somatic cell nuclear transfer and intracytoplasmic sperm injection. MATERIALS AND METHODS: In this experimental study, the application of phiC31 integrase system was evaluated for generating transgenic bovine embryos by somatic cell nuclear transfer (SCNT) and sperm mediated gene transfer (SMGT) approaches...
April 2018: Cell Journal
Dipesh Dhakal, Si-Kyu Lim, Dae Hee Kim, Byung-Gee Kim, Tokutaro Yamaguchi, Jae Kyung Sohng
Streptomyces peucetius ATCC 27952 is a filamentous soil bacterium with potential to produce anthracyclines such as doxorubicin (DXR) and daunorubicin (DNR), which are potent chemotherapeutic agents for the treatment of cancer. Here we present the complete genome sequence of S. peucetius ATCC 27952, which consists of 8,023,114 bp with a linear chromosome, 7187 protein-coding genes, 18 rRNA operons and 66 tRNAs. Bioinformatic analysis of the genome sequence revealed ∼68 putative gene clusters involved in the biosynthesis of secondary metabolites, including diverse classes of natural products...
January 4, 2018: Journal of Biotechnology
Dawn R D Bignell, Zhenlong Cheng, Luke Bown
Phytotoxins are secondary metabolites that contribute to the development and/or severity of diseases caused by various plant pathogenic microorganisms. The coronafacoyl phytotoxins are an important family of plant toxins that are known or suspected to be produced by several phylogenetically distinct plant pathogenic bacteria, including the gammaproteobacterium Pseudomonas syringae and the actinobacterium Streptomyces scabies. At least seven different family members have been identified, of which coronatine was the first to be described and is the best-characterized...
January 6, 2018: Antonie Van Leeuwenhoek
Magdalena Wypij, Joanna Czarnecka, Magdalena Świecimska, Hanna Dahm, Mahendra Rai, Patrycja Golinska
We report synthesis of silver nanoparticles (AgNPs) from Streptomyces xinghaiensis OF1 strain, which were characterised by UV-Vis and Fourier transform infrared spectroscopy, Zeta sizer, Nano tracking analyser, and Transmission electron microscopy. The antimicrobial activity of AgNPs alone, and in combination with antibiotics was evaluated against bacteria, namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis, and yeasts viz., Candida albicans and Malassezia furfur by using micro-dilution method...
January 5, 2018: World Journal of Microbiology & Biotechnology
Emily C Ulrich, Despina J Bougioukou, Wilfred A van der Donk
Dehydrophos is a tripeptide phosphonate antibiotic produced by Streptomyces luridus. Its biosynthetic pathway involves the use of aminoacyl-tRNA (aa-tRNA) for amide bond formation. The first amide bond during biosynthesis is formed by DhpH-C, a peptidyltransferase that utilizes Leu-tRNALeu. DhpH-C is a member of a burgeoning family of natural product biosynthetic enzymes that make use of aa-tRNA outside of canonical translation activities in the cell. Here we used site-directed mutagenesis of both DhpH-C and tRNALeu to investigate the enzyme mechanism and substrate specificity, respectively, and analyzed the substrate scope for the production of a series of dipeptides...
January 5, 2018: ACS Chemical Biology
Gongli Zong, Chuanqing Zhong, Jiafang Fu, Zhilong Zhao, Guangxiang Cao
Streptomyces gilvosporeus strain F607 is a producer of high levels of natamycin used in the fermentation industry. In this study, the complete genome sequence of strain F607 was determined. This genome sequence provides a basis for understanding natamycin biosynthesis and regulation in a high-natamycin-producing strain and will aid in the development of useful strategies for improving industrial strains.
January 4, 2018: Genome Announcements
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