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Streptomyces

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https://www.readbyqxmd.com/read/28526789/a-novel-iron-dependent-enzyme-that-catalyzes-the-initial-step-in-the-biodegradation-of-n-nitroglycine-by-variovorax-sp-strain-js1663
#1
Kristina M Mahan, Hangping Zheng, Tekle T Fida, Ronald J Parry, David E Graham, Jim C Spain
Nitramines are key constituents of most of the explosives in current use and consequently contaminate soil and groundwater at many military facilities around the world. Toxicity from nitramine contamination poses a health risk to plants and animals, thus understanding how nitramines are biodegraded is critical to environmental remediation. The biodegradation of synthetic nitramine compounds such as hexahydro-1,3,5-trinitro-1,3,5- triazine (RDX) has been studied for decades, but little is known about the catabolism of naturally produced nitramine compounds...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28523731/wble2-transcription-factor-in-streptomyces-griseus-s4-7-plays-an-important-role-in-plant-protection
#2
Hyun Ji Cho, Young Sang Kwon, Da-Ran Kim, Gyeongjun Cho, Seong Won Hong, Dong-Won Bae, Youn-Sig Kwak
Streptomyces griseus S4-7 was originally isolated from the strawberry rhizosphere as a microbial agent responsible for Fusarium wilt suppressive soils. S. griseus S4-7 shows specific and pronounced antifungal activity against Fusarium oxysporum f. sp. fragariae. In the Streptomyces genus, the whi transcription factors are regulators of sporulation, cell differentiation, septation, and secondary metabolites production. wblE2 function as a regulator has emerged as a new group in whi transcription factors. In this study, we reveal the involvement of the wblE2 transcription factor in the plant-protection by S...
May 19, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28522727/draft-genome-sequence-of-the-tacrolimus-producing-bacterium-streptomyces-tsukubaensis-f601
#3
Gongli Zong, Chuanqing Zhong, Jiafang Fu, Ronghuo Qin, Guangxiang Cao
Streptomyces tsukubaensis strain F601 was found to be a producer of the immunosuppressive drug tacrolimus. The draft genome sequence of this strain was approximately 8.52 Mbp. Genes involved in the biosynthesis of tacrolimus were identified in the genome. This draft genome sequence will provide insights into the genetic basis of tacrolimus biosynthesis and regulation.
May 18, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28522606/structural-analysis-of-the-dual-function-thioesterase-sav606-unravels-the-mechanism-of-michael-addition-of-glycine-to-an-%C3%AE-%C3%AE-unsaturated-thioester
#4
Taichi Chisuga, Akimasa Miyanaga, Fumitaka Kudo, Tadashi Eguchi
Thioesterases catalyze hydrolysis of acyl thioesters to release carboxylic acid or macrocyclization to produce the corresponding macrocycle in the biosynthesis of fatty acids, polyketides, or nonribosomal peptides. Recently, we reported that the thioesterase CmiS1 from Streptomyces sp. MJ635-86F5 catalyzes the Michael addition of glycine to an α,β-unsaturated fatty acyl thioester followed by thioester hydrolysis in the biosynthesis of the macrolactam antibiotic cremimycin. However, the molecular mechanisms of CmiS1-catalyzed reactions are unclear...
May 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28522365/knowns-and-unknowns-of-membrane-lipid-synthesis-in-streptomycetes
#5
Mario Sandoval-Calderón, Ziqiang Guan, Christian Sohlenkamp
Bacteria belonging to the genus Streptomyces are among the most prolific producers of antibiotics. Research on cellular membrane biosynthesis and turnover is lagging behind in Streptomyces compared to related organisms like Mycobacterium tuberculosis. While natural products discovery in Streptomyces is evidently a priority in order to discover new antibiotics to combat the increase in antibiotic resistant pathogens, a better understanding of this cellular compartment should provide insights into the interplay between core and secondary metabolism...
May 15, 2017: Biochimie
https://www.readbyqxmd.com/read/28521222/flocculation-mechanism-of-the-actinomycete-streptomyces-sp-hsn06-on-chlorella-vulgaris
#6
Yi Li, Yanting Xu, Tianling Zheng, Hailei Wang
In this study, an actinomycete Streptomyces sp. hsn06 with the ability to harvest Chlorella vulgaris biomass was used to investigate the flocculation mechanism. Streptomyces sp. hsn06 exhibited flocculation activity on algal cells through mycelial pellets with adding calcium. Calcium was determined to promote flocculation activity of mycelial pellets as a bridge binding with mycelial pellets and algal cells, which implied that calcium bridging is the main flocculation mechanism for mycelial pellets. Characteristics of flocculation activity confirmed proteins in mycelial pellets involved in flocculation procedure...
May 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28520425/furan-and-lactam-jadomycin-biosynthetic-congeners-isolated-from-streptomyces-venezuelae-isp5230-cultured-with-n%C3%AE%C2%B5-trifluoroacetyl-l-lysine
#7
Stephanie M Forget, Andrew W Robertson, David P Overy, Russell G Kerr, David L Jakeman
Angucycline antibiotics are composed of a classical four-ring angularly linked polyaromatic backbone. Differential cyclization chemistry of the A- and B-rings in jadomycin biosynthesis led to the discovery of two new furan analogues, while oxidation led to a ring-opened form of the jadomycin Nε-trifluoroacetyl-l-lysine (TFAL) congener. The compounds were isolated from Streptomyces venezuelae ISP5230 cultures grown with TFAL. Biosynthetic incorporation using d-[1-(13)C]-glucose in cultures enabled the unambiguous assignment of the aldehyde, alcohol, and amide functionalities present in these new congeners through NMR spectroscopy...
May 18, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28516483/capabilities-and-limitations-of-dgge-for-the-analysis-of-hydrocarbonoclastic-prokaryotic-communities-directly-in-environmental-samples
#8
Dina M Al-Mailem, Mayada K Kansour, Samir S Radwan
Prokaryotic communities in pristine and oil-contaminated desert soil, seawater, and hypersaline coastal soil were analyzed using culture-dependent and culture-independent approaches. The former technique was the dilution-plating method. For the latter, total genomic DNA was extracted and the 16S rRNA genes were amplified using a universal bacterial primer pair and primer pairs specific for Actinobacteria, Gammaproteobacteria, and Archaea. The amplicons were resolved using denaturing gradient gel electrophoresis (DGGE) and sequenced, and the sequences were compared to those in GenBank...
May 18, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28516055/antimicrobial-and-enzymatic-activity-of-actinomycetes-isolated-from-soils-of-coastal-islands
#9
Ivana Charousová, Juraj Medo, Eva Halenárová, Soňa Javoreková
Microbiological investigation of unexplored ecosystems is crucial for discovering of antibiotic producing actinomycetes. The present study was conducted to determine antimicrobial activity and identify the most active strains. Actinomycetes were isolated using the spread plate technique following by serial dilution of samples on starch casein agar. The screening method consists of primary and secondary testing. The most active isolates were identified based on molecular and cultural methods. 42 out of 66 isolates displayed antimicrobial potential...
April 2017: Journal of Advanced Pharmaceutical Technology & Research
https://www.readbyqxmd.com/read/28515390/biocontrol-potential-of-an-endophytic-streptomyces-sp-strain-mbcn152-1-against-alternaria-brassicicola-on-cabbage-plug-seedlings
#10
Naglaa Hassan, Satoko Nakasuji, Mohsen Mohamed Elsharkawy, Hushna Ara Naznin, Masaharu Kubota, Hammad Ketta, Masafumi Shimizu
In the present study, 77 strains of endophytic actinomycetes isolated from cabbage were screened in order to assess their biocontrol potential against Alternaria brassicicola on cabbage seedlings. In the first and second screening trials, cabbage seedlings pretreated with mycelial suspensions of each isolate were spray-inoculated with A. brassicicola. Strain MBCN152-1, which exhibited the best protection in screening trials and had no adverse effects on seedling growth, was selected for the greenhouse trial...
May 18, 2017: Microbes and Environments
https://www.readbyqxmd.com/read/28512573/photoreduction-and-validation-of-haem-ligand-intermediate-states-in-protein-crystals-by-in-situ-single-crystal-spectroscopy-and-diffraction
#11
Demet Kekilli, Tadeo Moreno-Chicano, Amanda K Chaplin, Sam Horrell, Florian S N Dworkowski, Jonathan A R Worrall, Richard W Strange, Michael A Hough
Powerful synergies are available from the combination of multiple methods to study proteins in the crystalline form. Spectroscopies which probe the same region of the crystal from which X-ray crystal structures are determined can give insights into redox, ligand and spin states to complement the information gained from the electron-density maps. The correct assignment of crystal structures to the correct protein redox and ligand states is essential to avoid the misinterpretation of structural data. This is a particular concern for haem proteins, which can occupy a wide range of redox states and are exquisitely sensitive to becoming reduced by solvated electrons generated from interactions of X-rays with water molecules in the crystal...
May 1, 2017: IUCrJ
https://www.readbyqxmd.com/read/28508386/application-of-response-surface-methodology-for-optimization-of-medium-components-for-the-production-of-secondary-metabolites-by-streptomyces-diastatochromogenes-kx852460
#12
Taswar Ahsan, Jianguang Chen, Yuanhua Wu, Muhammad Irfan
A bioactive strain Streptomyces diastatochromogenes KX852460 was selected for the production of secondary metabolites to control the target spot disease on tobacco leaves, caused by the Rhizoctonia solani AG-3. Peanut meal, soluble starch, NaCl, yeast extract, and ammonium sulphate were identified the best ingredient for high antifungal activity of S. diastatochromogenes KX852460 against the R. solani AG-3. For the improved production of secondary metabolites, central composite design of response surface methodology was applied in submerged fermentation...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28507698/formicamycins-antibacterial-polyketides-produced-by-streptomyces-formicae-isolated-from-african-tetraponera-plant-ants
#13
Zhiwei Qin, John T Munnoch, Rebecca Devine, Neil A Holmes, Ryan F Seipke, Karl A Wilkinson, Barrie Wilkinson, Matthew I Hutchings
We report a new Streptomyces species named S. formicae that was isolated from the African fungus-growing plant-ant Tetraponera penzigi and show that it produces novel pentacyclic polyketides that are active against MRSA and VRE. The chemical scaffold of these compounds, which we have called the formicamycins, is similar to the fasamycins identified from the heterologous expression of clones isolated from environmental DNA, but has significant differences that allow the scaffold to be decorated with up to four halogen atoms...
April 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28507537/deep-sea-actinomycetes-and-their-secondary-metabolites
#14
REVIEW
Manita Kamjam, Periyasamy Sivalingam, Zinxin Deng, Kui Hong
Deep sea is a unique and extreme environment. It is a hot spot for hunting marine actinomycetes resources and secondary metabolites. The novel deep sea actinomycete species reported from 2006 to 2016 including 21 species under 13 genera with the maximum number from Microbacterium, followed by Dermacoccus, Streptomyces and Verrucosispora, and one novel species for the other 9 genera. Eight genera of actinomycetes were reported to produce secondary metabolites, among which Streptomyces is the richest producer...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28504888/cytotoxic-bagremycins-from-mangrove-derived-streptomyces-sp-q22
#15
Lei Chen, Weiyun Chai, Wenling Wang, Tengfei Song, Xiao-Yuan Lian, Zhizhen Zhang
New bagremycins C-E (3-5) and bagrelactone A (6), together with known bagremycins A (1) and B (2), 4-hydroxystyrene (7), and 4-hydroxystyrene 4-O-α-d-galactopyranoside (8), were isolated from a mangrove-derived actinomycete, Streptomyces sp. Q22. Structures of these new compounds were elucidated based on their NMR and HRESIMS spectroscopic data as well as chemical degradation. Bagremycin C (3) is a unique analogue with an N-acetyl-(S)-cysteine moiety, while bagrelactone A (6) represents the first example of this type of bagremycin-derived macrolide...
May 15, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28502957/1-methoxypyrrole-2-carboxamide-a-new-pyrrole-compound-isolated-from-streptomyces-griseocarneus-sww368
#16
Watsapon Wattanasuepsin, Bungonsiri Intra, Jirayut Euanorasetr, Yoshihiro Watanabe, Ratchanee Mingma, Wataru Fukasawa, Mihoko Mori, Atsuko Matsumoto, Kazuro Shiomi, Watanalai Panbangred
A new pyrrole compound, 1-methoxypyrrole-2-carboxamide, was obtained from a culture broth of Streptomyces griseocarneus SWW368, which was isolated from the rhizospheric soil under a Para rubber tree (Hevea brasiliensis). The chemical structure was elucidated by 1D NMR, 2D NMR, and MS, as a pyrrole ring with a N-methoxy group and a primary amide group. It exhibited antibacterial properties against Kocuria rhizophila, Staphylococcus aureus and Xanthomonas campestris pv. oryzae; however, cytotoxicity of the compound at 714 μM against several mammalian tumor cell lines, i...
May 15, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28500722/uv-resistant-actinobacteria-from-high-altitude-andean-lakes-isolation-characterization-and-antagonistic-activities
#17
María Cecilia Rasuk, Gabriela Mónica Ferrer, Daniel Kurth, Luciano Raúl Portero, María Eugenia Farías, Virginia Helena Albarracín
Polyextremophiles are present in a wide variety of extreme environments in which they must overcome various hostile conditions simultaneously such as high UVB radiation, extreme pHs and temperatures, elevated salt and heavy-metal concentration, low-oxygen pressure and scarce nutrients. High-altitude Andean lakes (HAALs; between 2000 and 4000 m) are one example of these kinds of ecosystems suffering from the highest total solar and UVB radiation on Earth where an abundant and diverse polyextremophilic microbiota was reported...
May 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28499263/effect-of-fumigation-with-chloropicrin-on-soil-bacterial-communities-and-genes-encoding-key-enzymes-involved-in-nitrogen-cycling
#18
Jun Li, Bin Huang, Qiuxia Wang, Yuan Li, Wensheng Fang, Dongdong Yan, Meixia Guo, Aocheng Cao
Chloropicrin (CP) is a potential alternative for methyl bromide as a soil fumigant given that the use of methyl bromide has become limited. However, little is known about how fumigation with CP affects the condition of the soil microbial community. In this study, 16S rRNA amplicon sequencing and quantitative PCR were combined to investigate the effect of CP on soil bacterial community. In total, 938,922 effective reads were obtained from 18 samples and clustered into 58,662 operational taxonomic units at a similarity cut-off of 97%...
May 9, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28497964/biosynthetic-origin-of-the-hydroxamic-acid-moiety-of-trichostatin-a-identification-of-unprecedented-enzymatic-machinery-involved-in-hydroxylamine-transfer
#19
Kei Kudo, Taro Ozaki, Kazuo Shin-Ya, Makoto Nishiyama, Tomohisa Kuzuyama
Trichostatin A (TSA) is widely used in the field of epigenetics because it potently inhibits histone deacetylase (HDAC). In-depth studies have revealed that the hydroxamic acid group in TSA chelates the zinc(II) ion in the active site of HDAC to realize the inhibitory activity. Here we report the first identification of a complete TSA biosynthetic gene cluster from Streptomyces sp. RM72 and the heterologous production of TSA in Streptomyces albus. Biochemical analyses unambiguously demonstrate that unprecedented biosynthetic machinery catalyzes the direct transfer of hydroxylamine from a nonproteinogenic amino acid, l-glutamic acid γ-monohydroxamate, to the carboxylic acid group of trichostatic acid to form the hydroxamic acid moiety of TSA...
May 12, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28497061/mining-of-microbial-genomes-for-the-novel-sources-of-nitrilases
#20
Nikhil Sharma, Neerja Thakur, Tilak Raj, Savitri, Tek Chand Bhalla
Next-generation DNA sequencing (NGS) has made it feasible to sequence large number of microbial genomes and advancements in computational biology have opened enormous opportunities to mine genome sequence data for novel genes and enzymes or their sources. In the present communication in silico mining of microbial genomes has been carried out to find novel sources of nitrilases. The sequences selected were analyzed for homology and considered for designing motifs. The manually designed motifs based on amino acid sequences of nitrilases were used to screen 2000 microbial genomes (translated to proteomes)...
2017: BioMed Research International
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