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Streptomyces

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https://www.readbyqxmd.com/read/28445069/targeted-dereplication-of-microbial-natural-products-by-high-resolution-ms-and-predicted-lc-retention-time
#1
Justine Chervin, Marc Stierhof, Ming Him Tong, Doe Peace, Kine Østnes Hansen, Dagmar Solveig Urgast, Jeanette Hammer Andersen, Yi Yu, Rainer Ebel, Kwaku Kyeremeh, Veronica Paget, Gabriela Cimpan, Albert Van Wyk, Hai Deng, Marcel Jaspars, Jioji N Tabudravu
A new strategy for the identification of known compounds in Streptomyces extracts that can be applied in the discovery of natural products is presented. The strategy incorporates screening a database of 5555 natural products including 5098 structures from Streptomyces sp., using a high-throughput LCMS data processing algorithm that utilizes HRMS data and predicted LC retention times (tR) as filters for rapid identification of known compounds in the natural product extract. The database, named StrepDB, contains for each compound the structure, molecular formula, molecular mass, and predicted LC retention time...
April 26, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28444602/bioactive-guided-fractionation-of-diols-from-streptomyces-sp-msl
#2
Raja Hima Bindhu Madasu, Vijayalakshmi Muvva, Rajesh Kumar Munaganti, Kumar Reddy Dorigondla, Venkateswarlu Yenamandra
An actinomycete strain with a great potential to produce bioactive compounds isolated from a laterite soil was identified as Streptomyces sp. MSL based on 16S rDNA sequence analysis. Secondary metabolites produced by the strain in optimized nutrient broth were extracted and analyzed by chromatographic and spectroscopic techniques. Among the different fractions, four diols, viz., (1) (2R,3R)-2,3-Butanediol, (2) (2R,3S)-2,3-Butanediol, (3) 2,3-dimethyl-2,3-butanediol (Pinacol), and (4) (3R)-1,3-Butanediol exhibited good antimicrobial activity...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28444566/improvement-of-rice-seedling-growth-and-nitrogen-use-efficiency-by-seed-inoculation-with-endophytic-denitrifiers
#3
Wenfeng Wang, Yanyan Zhai, Lixiang Cao, Hongming Tan, Renduo Zhang
The aim of the present study is to investigate the effect of seed inoculation with endophytic denitrifiers on rice seedling growth and nitrogen use efficiency under low- and high-urea conditions. Pseudomonas sp. B2, Streptomyces sp. A9, and Fusarium sp. F3 were isolated from rice plant tissues. Rice seeds inoculated with the denitrifiers were sown in soil fertilized with 100 and 300 mg/kg urea concentrations, respectively. The denitrifiers increased soil ammonia concentrations or kept high ammonia concentration for a longer time in soils...
April 25, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28443932/nucleoside-linked-shunt-products-in-the-reaction-catalyzed-by-the-class-c-radical-s-adenosylmethionine-methyltransferase-nosn
#4
Wei Ding, Yujie Wu, Xinjian Ji, Haocheng Qianzhu, Fener Chen, Zixin Deng, Yi Yu, Qi Zhang
NosN is a class C radical S-adenosylmethionine (SAM) methyltransferase (RSMT) involved in the biosynthesis of nosiheptide, a clinically interesting thiopeptide antibiotic produced by Streptomyces actuosus. NosN employs an unprecedented catalytic mechanism, in which SAM is converted to 5'-methylthioadenosine (MTA) as a direct methyl donor. In this study, we report identification of several nucleoside-linked shunt products in the NosN-catalyzed reaction. Comparative analysis of NosN and the class A RSMT RlmN and further density functional theory (DFT) calculations reveal important mechanistic insights into the catalyses of the two types of enzymes, showing that the radical intermediates generated by similar pathways can have very diverse reactivities...
April 26, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28442735/novel-antibiotic-screening-methods-to-awaken-silent-or-cryptic-secondary-metabolic-pathways-in-actinomycetes
#5
REVIEW
Hiroyasu Onaka
Streptomyces have the potential to produce more than 30 secondary metabolites; however, the expression of most metabolite biosynthetic gene clusters is cryptic or silent. Indeed, the expression of these genes is conditional and dependent on culture conditions. To activate such gene clusters, many methods have been developed. In this review, I describe two activation approaches developed in my laboratory: use of the activation mediator goadsporin and combined-culture. Goadsporin is a chemical substance isolated from Streptomyces sp...
April 26, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28441878/four-new-anthraquinones-from-a-soil-actinomycete-streptomyces-sp-ws-13394-and-their-bioactivities
#6
Zhaoyuan Wu, Yani Zhang, Wei Fang, Liqiao Shi, Zhongyi Wan
Further chemical study of secondary metabolites from the soil actinomycete Streptomyces sp. WS-13394 resulted in the isolation of four new alkylated anthraquinone analogues (5-8). Their structures were elucidated on the basis of extensive spectroscopic analysis, including HR-ESI-MS, 1D and 2D NMR. The new compounds, together with analogues obtained before (1-4), were tested for their in vitro cytotoxicity against Huh-7 and SGC-7901.
April 25, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28439850/heterologous-expression-of-a-streptomyces-cyaneus-laccase-for-biomass-modification-applications
#7
Selin Ece, Camilla Lambertz, Rainer Fischer, Ulrich Commandeur
Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing Streptomyces cyaneus CECT 3335 laccase in Escherichia coli (ScLac) and we achieved a yield of up to 104 mg L(-1) following purification by one-step affinity chromatography. Stability and activity assays using simple lignin model substrates showed that the purified enzyme preparation was active over a broad pH range and at high temperatures, suggesting it would be suitable for biomass degradation...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28439624/evidence-for-the-formation-of-scbr-scbr2-heterodimers-and-identification-of-one-of-the-regulatory-targets-in-streptomyces-coelicolor
#8
Xiao Li, Juan Wang, Mingxin Shi, Weishan Wang, Christophe Corre, Keqian Yang
The homologous transcriptional regulators ScbR and ScbR2 have previously been identified as γ-butyrolactone (GBL) and antibiotic receptors, respectively. They regulate diverse physiological processes in Streptomyces coelicolor in response to GBL and antibiotic signals. In this study, ScbR and ScbR2 proteins were shown to interact using a bacterial two-hybrid system where adenylate cyclase activity was reconstituted in Escherichia coli BTH101. These ScbR/ScbR2 interactions in S. coelicolor were then demonstrated by co-immunoprecipitation...
April 24, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28438038/bisamides-and-rhamnosides-from-mangrove-actinomycete-streptomyces-sp-sz-a15
#9
Qiaoqiao Ma, Wanjing Ding, Zhe Chen, Zhongjun Ma
Four new bisamides 1-4, and two new rhamnosides (5, 6), along with four known compounds 7-10, were isolated from a scale culture of the mangrove-derived actinomycete Streptomyces sp. SZ-A15. All structures were determined through analysis of the UV, IR, HRESIMS, 1D and 2D NMR spectra as well as by comparison with literature data. BRD4 inhibition of all isolated compounds was evaluated. As for the ability to inhibit protein BRD4, compound 9 exhibited moderate activity with the value of 78.4 ± 2.2% at 10 μM...
April 24, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28437452/enhanced-antibacterial-metabolite-production-through-the-application-of-statistical-methodologies-by-a-streptomyces-nogalater-niist-a30-isolated-from-western-ghats-forest-soil
#10
Jubi Jacob, Reshma Uma Rajendran, Syama Hari Priya, Jayamurthy Purushothaman, Dileep Kumar Bhaskaran Nair Saraswathy Amma
Streptomyces strains isolated from Nelliyampathy forest soil of Western Ghats, Kerala, India were evaluated for their antibacterial efficacy against two indicator pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Among 140 strains tested, sixteen recorded potent antibacterial properties and were further screened against eleven bacterial pathogens. A strain identified as Streptomyces nogalater and designated as NIIST A30 exhibited maximum inhibition against all the test pathogens. Among the eight fermentation media tested, inorganic salts starch broth recorded the best for antibacterial production...
2017: PloS One
https://www.readbyqxmd.com/read/28436673/unique-rhizosphere-micro-characteristics-facilitate-phytoextraction-of-multiple-metals-in-soil-by-the-hyperaccumulating-plant-sedum-alfredii
#11
Dandi Hou, Kai Wang, Ting Liu, Haixin Wang, Zhi Lin, Jie Qian, Lingli Lu, Shengke Tian
Understanding the strategies that the roots of hyperaccumulating plants use to extract heavy metals from soils is important for optimizing phytoremediation. The rhizosphere characteristics of Sedum alfredii, a hyperaccumulator, were investigated 6 months after planting in weathered field soils contaminated with 5.8 μg·g-1 Cd, 1985.1 μg·g-1 Zn, 667.5 μg·g-1 Pb, and 698.8 μg·g-1 Cu. In contrast with the non-hyperaccumulating ecotype (NHE), the hyperaccumulating ecotype (HE) of S. alfredii was more tolerant to the metals and accumulated higher levels of Cd and Zn...
April 24, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28436005/fast-and-reliable-strain-characterization-of-streptomyces-lividans-through-micro-scale-cultivation
#12
Joachim Koepff, Matthias Keller, C Konstantinos Tsolis, Tobias Busche, Christian Rückert, Mohamed B Hamed, Jozef Anné, Joern Kalinowski, Wolfgang Wiechert, Anastassios Economou, Marco Oldiges
Filamentous organisms of the genus Streptomyces play an important role in industrial production processes, due to their extensive secondary metabolism variability, as well as their ability to secrete efficiently large amounts of (heterologous) proteins. While genetic engineering tools are available to rapidly build up large strain libraries, the subsequent strain screening and bioprocess development still constitutes a bottleneck. This is due to the lack of reliable parallelized and accelerated cultivation techniques for morphologically challenging organisms...
April 24, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28435354/control-efficacy-of-streptomyces-sp-a501-against-ginseng-damping-off-and-its-antifungal-substance
#13
Nguyen Van Minh, E-Eum Woo, Gang-Seon Lee, Dae-Won Ki, In-Kyoung Lee, Sang-Yeob Lee, Kyeonghun Park, Jaekyeong Song, Jae Eul Choi, Bong-Sik Yun
Ginseng damping-off, caused by the fungal pathogens Rhizoctonia solani and Pythium sp., is a critical disease in ginseng seedling. In a continuing effort to find microorganisms with the potential of acting as a biocontrol agent against Rhizoctonia damping-off, we found that a Streptomyces sp. A501 showed significant antifungal activity against Rhizoctonia solani. In field experiment to test the efficacy of Streptomyces sp. A501 in controlling ginseng damping-off, the incidence of damping-off disease was meaningfully reduced when ginseng seeds were soaked in the culture broth of Streptomyces sp...
March 2017: Mycobiology
https://www.readbyqxmd.com/read/28435299/genetic-interrelations-in-the-actinomycin-biosynthetic-gene-clusters-of-streptomyces-antibioticus-imru-3720-and-streptomyces-chrysomallus-atcc11523-producers-of-actinomycin-x-and-actinomycin-c
#14
Ivana Crnovčić, Christian Rückert, Siamak Semsary, Manuel Lang, Jörn Kalinowski, Ullrich Keller
Sequencing the actinomycin (acm) biosynthetic gene cluster of Streptomyces antibioticus IMRU 3720, which produces actinomycin X (Acm X), revealed 20 genes organized into a highly similar framework as in the bi-armed acm C biosynthetic gene cluster of Streptomyces chrysomallus but without an attached additional extra arm of orthologues as in the latter. Curiously, the extra arm of the S. chrysomallus gene cluster turned out to perfectly match the single arm of the S. antibioticus gene cluster in the same order of orthologues including the the presence of two pseudogenes, scacmM and scacmN, encoding a cytochrome P450 and its ferredoxin, respectively...
2017: Advances and Applications in Bioinformatics and Chemistry: AABC
https://www.readbyqxmd.com/read/28425466/crystal-structures-of-the-no-sensor-nsrr-reveal-how-its-iron-sulfur-cluster-modulates-dna-binding
#15
Anne Volbeda, Erin L Dodd, Claudine Darnault, Jason C Crack, Oriane Renoux, Matthew I Hutchings, Nick E Le Brun, Juan C Fontecilla-Camps
NsrR from Streptomyces coelicolor (Sc) regulates the expression of three genes through the progressive degradation of its [4Fe-4S] cluster on nitric oxide (NO) exposure. We report the 1.95 Å resolution crystal structure of dimeric holo-ScNsrR and show that the cluster is coordinated by the three invariant Cys residues from one monomer and, unexpectedly, Asp8 from the other. A cavity map suggests that NO displaces Asp8 as a cluster ligand and, while D8A and D8C variants remain NO sensitive, DNA binding is affected...
April 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28422240/zwitterionic-pyrrolidene-phosphonates-inhibitors-of-the-glycoside-hydrolase-like-phosphorylase-streptomyces-coelicolor-glgei-v279s
#16
Sri Kumar Veleti, Cecile Petit, Donald R Ronning, Steven J Sucheck
We synthesized and evaluated new zwitterionic inhibitors against glycoside hydrolase-like phosphorylase Streptomyces coelicolor (Sco) GlgEI-V279S which plays a role in α-glucan biosynthesis. Sco GlgEI-V279S serves as a model enzyme for validated anti-tuberculosis (TB) target Mycobacterium tuberculosis (Mtb) GlgE. Pyrrolidine inhibitors 5 and 6 were designed based on transition state considerations and incorporate a phosphonate on the pyrrolidine moiety to expand the interaction network between the inhibitor and the enzyme active site...
April 19, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28421050/reveromycins-a-and-b-from-streptomyces-sp-3-10-antifungal-activity-against-plant-pathogenic-fungi-in-vitro-and-in-a-strawberry-food-model-system
#17
Ang Lyu, Hao Liu, Hongjie Che, Long Yang, Jing Zhang, Mingde Wu, Weidong Chen, Guoqing Li
This study was conducted to determine the antifungal activity of the metabolites from Streptomyces sp. 3-10, and to purify and identify the metabolites. Meanwhile, the taxonomic status of strain 3-10 was re-evaluated. The cultural filtrates of strain 3-10 in potato dextrose broth were extracted with ethyl acetate. The resulting crude extract at 1 and 5 μg/ml inhibited growth of 22 species in 18 genera of plant pathogenic fungi and Oomycetes, accounting for 92% of the total 24 tested species, suggesting that it has a wide antifungal spectrum...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28420869/verification-of-oligomycin-a-structure-synthesis-and-biological-evaluation-of-33-dehydrooligomycin-a
#18
Lyudmila N Lysenkova, Oleg Y Saveljev, Natalya E Grammatikova, Vladimir B Tsvetkov, Olga B Bekker, Valery N Danilenko, Lyubov G Dezhenkova, Eugene E Bykov, Olga A Omelchuk, Alexander M Korolev, Andrey E Shchekotikhin
Although, the structure of oligomycin A (1) was confirmed by spectroscopic and chemical evaluations, some crystallographic data cast doubt on the originally adopted structure of the side 2-hydroxypropyl moiety of this antibiotic. It was suggested that the side chain of the oligomycin is enol-related (2-hydroxy-1-propenyl). To clarify this matter we synthesized and evaluated 33-dehydrooligomycin A (2) prepared by the Kornblum oxidation of 33-O-mesyloligomycin A (3) by dimethyl sulfoxide. NMR data for 33-dehydrooligomycin (2) and results of quantum chemical calculations have shown that this derivative exists in the keto rather than in the enol tautomer 2a...
April 19, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28420868/aurachin-ss-a-new-antibiotic-from-streptomyces-sp-na04227
#19
Mei Zhang, Cheng Long Yang, Yong Sheng Xiao, Bo Zhang, Xin Zhao Deng, L I Yang, Jing Shi, Y I Shuang Wang, Wei Li, Rui Hua Jiao, Ren Xiang Tan, Hui Ming Ge
No abstract text is available yet for this article.
April 19, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28420658/simple-and-rapid-biochemical-method-to-synthesize-labeled-or-unlabeled-phosphatidylinositol-species
#20
Satu Hänninen, Krishna Chaithanya Batchu, Kati Hokynar, Pentti Somerharju
Phosphatidylinositol (PI) is the precursor of many important signaling molecules in eukaryotic cells and, most probably, PI also has important functions in cellular membranes. However, these functions are poorly understood, which is largely due to that (i) only few PI species with specific acyl chains are available commercially and (ii) there are no simple methods to synthesize such species. Here, we present a simple biochemical method to synthetize a variety of labeled or unlabeled PI species from corresponding commercially available phosphatidylcholines...
April 18, 2017: Journal of Lipid Research
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