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https://www.readbyqxmd.com/read/28098172/structural-diversity-of-anti-pancreatic-cancer-capsimycins-identified-in-mangrove-derived-streptomyces-xiamenensis-318-and-post-modification-via-a-novel-cytochrome-p450-monooxygenase
#1
He-Lin Yu, Shu-Heng Jiang, Xu-Liang Bu, Jia-Hua Wang, Jing-Yi Weng, Xiao-Mei Yang, Kun-Yan He, Zhi-Gang Zhang, Ping Ao, Jun Xu, Min-Juan Xu
Polycyclic tetramate macrolactams (PTMs) were identified as distinct secondary metabolites of the mangrove-derived Streptomyces xiamenensis 318. Together with three known compounds-ikarugamycin (1), capsimycin (2) and capsimycin B (3)-two new compounds, capsimycin C (4) with trans-diols and capsimycin D (5) with trans-configurations at C-13/C-14, have been identified. The absolute configurations of the tert/tert-diols moiety was determined in 4 by NMR spectroscopic analysis, CD spectral comparisons and semi-synthetic method...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28097821/development-of-actinobacterial-resources-for-functional-cosmetics
#2
Ram Hari Dahal, Dong Seop Shim, Jaisoo Kim
BACKGROUND: Actinobacteria usually produce different functional compounds for various applications. OBJECTIVE: The aim of this research was to develop actinobacterial resources through the isolation and identification of soil bacteria with antibacterial and enzyme inhibitory activities for cosmetics application. METHODS: Soil bacteria were isolated and tested for antibacterial activity against Propionibacterium acnes and Staphylococcus epidermidis using the spotting method...
January 17, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28096548/the-synthesis-and-evaluation-of-the-antiproliferative-activity-of-deacidified-gex1a-analogues
#3
Takamichi Imaizumi, Hiroshi Nakagawa, Ran Hori, Yasuo Watanabe, Shiro Soga, Kyoichiro Iida, Hideyuki Onodera
GEX1A/herboxidiene (1) is a natural product isolated from Streptomyces sp. and has been reported to target the pre-mRNA splicing process. Although 1 was shown to have antitumor activity in vivo, weight loss was observed in mice when 1 was consecutively administered. We assumed that the carboxylic acid moiety was one of the causes of this toxicity. In this study, a series of amide, carbamate and urea analogues of 1 were synthesized and their antiproliferative activity was evaluated in vitro. The synthesis of urea analogues featured Curtius rearrangement following amine treatment with the one-pot procedure from 1...
January 18, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28096544/simamycin-5-o-geranyluridine-a-new-prenylated-nucleoside-from-streptomyces-sp
#4
Yasuhiro Igarashi, Takayuki Kyoso, Youngju Kim, Tsutomu Oikawa
A new nucleoside modified by prenylation, simamycin (1), was isolated from the culture broth of a soil-derived Streptomyces sp. Its structure was determined by spectroscopic analysis and chemical synthesis as 5'-O-geranyluridine. Compound 1 induced differentiation of preadipocytes into matured adipocytes.The Journal of Antibiotics advance online publication, 18 January 2017; doi:10.1038/ja.2016.163.
January 18, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28095865/engineered-biosynthesis-of-milbemycins-in-the-avermectin-high-producing-strain-streptomyces-avermitilis
#5
Myoun-Su Kim, Wan-Je Cho, Myoung Chong Song, Seong-Whan Park, Kaeun Kim, Eunji Kim, Naryeong Lee, Sang-Jip Nam, Ki-Hoon Oh, Yeo Joon Yoon
BACKGROUND: Milbemycins, produced from Streptomyces hygroscopicus subsp. aureolacrimosus and Streptomyces bingchenggensis, are 16-membered macrolides that share structural similarity with avermectin produced from Streptomyces avermitilis. Milbemycins possess strong acaricidal, insecticidal, and anthelmintic activities but low toxicity. Due to the high commercial value of the milbemycins and increasing resistance to the avermectins and their derivatives, it is imperative to develop an efficient combinatorial biosynthesis system exploiting an overproduction host strain to produce the milbemycins and novel analogs in large quantities...
January 17, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28095182/effect-of-intermittent-aeration-on-microbial-diversity-in-an-intermittently-aerated-ifas-reactor-treating-municipal-wastewater-a-field-study
#6
Akansha Bhatia, Nitin Kumar Singh, Timsi Bhando, Ranjana Pathania, Absar Ahmad Kazmi
In the present study, the effect of three intermittent aeration (IA) cycles on treatment performance and microbial diversity was investigated in an integrated fixed film activated sludge (IFAS) reactor treating municipal wastewater. The results showed that IA strategies were able to achieve efficient removal of organics and nitrogen ranging between 90 and 95% and 70 and 80%, respectively, however the phosphorus removal was found to be inversely proportional to the duration of aeration off time in each IA cycle...
January 17, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28094796/the-evolution-of-no-cost-resistance-at-sub-mic-concentrations-of-streptomycin-in-streptomyces-coelicolor
#7
Sanne Westhoff, Tim Marijn van Leeuwe, Omar Qachach, Zheren Zhang, Gilles Philippus van Wezel, Daniel Eric Rozen
At the high concentrations used in medicine, antibiotics exert strong selection on bacterial populations for the evolution of resistance. However, these lethal concentrations may not be representative of the concentrations bacteria face in soil, a recognition that has led to questions of the role of antibiotics in soil environments as well as the dynamics of resistance evolution during sublethal challenge. Here we examine the evolution of resistance to sub-minimal inhibitory concentrations (sub-MIC) of streptomycin in the filamentous soil bacterium Streptomyces coelicolor...
January 17, 2017: ISME Journal
https://www.readbyqxmd.com/read/28093673/metagenomic-insights-into-effects-of-spent-engine-oil-perturbation-on-the-microbial-community-composition-and-function-in-a-tropical-agricultural-soil
#8
Lateef B Salam, Sunday O Obayori, Francisca O Nwaokorie, Aisha Suleiman, Raheemat Mustapha
Analyzing the microbial community structure and functions become imperative for ecological processes. To understand the impact of spent engine oil (SEO) contamination on microbial community structure of an agricultural soil, soil microcosms designated 1S (agricultural soil) and AB1 (agricultural soil polluted with SEO) were set up. Metagenomic DNA extracted from the soil microcosms and sequenced using Miseq Illumina sequencing were analyzed for their taxonomic and functional properties. Taxonomic profiling of the two microcosms by MG-RAST revealed the dominance of Actinobacteria (23...
January 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28093470/active-site-maturation-and-activity-of-the-copper-radical-oxidase-glxa-is-governed-by-a-tryptophan-residue
#9
Amanda K Chaplin, Dimitri A Svistunenko, Michael Hough, Michael T Wilson, Erik Vijgenboom, Jonathan Ar Worrall
GlxA from Streptomyces lividans is a mononuclear copper-radical oxidase and a member of the auxiliary activity family 5 (AA5). Its domain organisation and low sequence homology make it a distinct member of the AA5 family in which the fungal galactose 6-oxidase (Gox) is the best-characterized. GlxA is a key cuproenzyme in the copper-dependent morphological development of S. lividans with a function that is linked to the processing of an extracytoplasmic glycan. The catalytic site in GlxA and Gox contain two distinct one-electron acceptors comprising the copper ion and a 3'-(S-cysteinyl) tyrosine...
January 16, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28091548/genome-analysis-of-a-zygomycete-fungus-choanephora-cucurbitarum-elucidates-necrotrophic-features-including-bacterial-genes-related-to-plant-colonization
#10
Byoungnam Min, Ji-Hyun Park, Hongjae Park, Hyeon-Dong Shin, In-Geol Choi
A zygomycete fungus, Choanephora cucurbitarum is a plant pathogen that causes blossom rot in cucurbits and other plants. Here we report the genome sequence of Choanephora cucurbitarum KUS-F28377 isolated from squash. The assembled genome has a size of 29.1 Mbp and 11,977 protein-coding genes. The genome analysis indicated that C. cucurbitarum may employ a plant pathogenic mechanism similar to that of bacterial plant pathogens. The genome contained 11 genes with a Streptomyces subtilisin inhibitor-like domain, which plays an important role in the defense against plant immunity...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28089379/characterization-of-a-novel-dyp-type-peroxidase-from-streptomyces-avermitilis
#11
Kanako Sugawara, Yuriko Nishihashi, Tomomi Narioka, Toru Yoshida, Mifumi Morita, Yasushi Sugano
DyP-type peroxidases are a heme peroxidase family with unique properties whose members are widely distributed from prokaryotes to eukaryotes. DyP-type peroxidases are subdivided into class P, I and V based on structure-based sequence alignment. Class V enzymes possess degradation activities for anthraquinone dyes, and include extra sequences compared with class P and I. Class V enzymes are mainly found in fungi, with only two such proteins, AnaPX and DyP2, reported in bacteria. Here, we heterologously expressed, purified and biochemically characterized SaDyP2 protein, predicted to belong to class V...
January 11, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28088540/multiplexed-site-specific-genome-engineering-for-overproducing-bioactive-secondary-metabolites-in-actinomycetes
#12
Lei Li, Guosong Zheng, Jun Chen, Mei Ge, Weihong Jiang, Yinhua Lu
Actinomycetes produce a large variety of pharmaceutically active compounds, yet production titers often require to be improved for discovery, development and large-scale manufacturing. Here, we describe a new technique, multiplexed site-specific genome engineering (MSGE) via the 'one integrase-multiple attB sites' concept, for the stable integration of secondary metabolite biosynthetic gene clusters (BGCs). Using MSGE, we achieved five-copy chromosomal integration of the pristinamycin II (PII) BGC in Streptomyces pristinaespiralis, resulting in the highest reported PII titers in flask and batch fermentations (2...
January 11, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28088096/mycelium-transformation-of-streptomyces-toxytricini-into-pellet-role-of-culture-conditions-and-kinetics
#13
Punit Kumar, Kashyap Kumar Dubey
The present study envisages the role of different carbon sources, nitrogen sources, metals, pH, inoculum volume and agitation rate in pellet formation of S. toxytricini at shake-flask level. It was found that galactose, ammonium sulphate, sodium nitrate, Cu(2+), Zn(2+), higher inoculum volume (5% v/v) and agitation rate at 300rpm caused significant reduction in pellet size (up to the range of 30μm-0.5mm) but biomass formations was also reduced subsequently. Interestingly diffused type of morphology was obtained in Fe(2+) supplemented medium with reduced biomass (1...
January 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28087615/microbial-catabolism-of-sterols-focus-on-the-enzymes-that-transform-the-sterol-3%C3%AE-hydroxy-5-en-into-3-keto-4-en
#14
Joseph Kreit
An overview on the microbial sterol catabolism is described with a focus on the catabolic step of the 3β-hydroxy-5-en structure. Cholesterol oxidase transforms this structure into the corresponding 3-keto-4-en feature, and thus initiates the sterol molecule catabolism. The oxidase has been found in a large number of microorganisms, especially in actinobacteria as species of Rhodococcus and Streptomyces Other actinobacteria as species of Mycobacterium and Nocardia possess NAD(P)-dependent dehydrogenase for this catabolic step...
January 12, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28087533/polysaccharide-degradation-capability-of-actinomycetales-soil-isolates-from-a-semi-arid-grassland-of-the-colorado-plateau
#15
Chris M Yeager, La Verne Gallegos-Graves, John Dunbar, Cedar N Hesse, Hajnalka Daligault, Cheryl R Kuske
: Among the bacteria, members of the Actinomycetales are considered quintessential degraders of complex polysaccharides in soils. However, studies examining complex polysaccharide degradation by Actinomycetales (other than Streptomyces spp.) in soils are limited. Here, we examine the lignocellulolytic and chitinolytic potential of 112 Actinomycetales strains, encompassing 13 families, isolated from a semi-arid grassland of the Colorado Plateau, Utah, USA. Members of the Streptomycetaceae, Pseudonocardiaceae, Micromonosporaceae and Promicromonosporaceae exhibited robust activity against carboxymethylcellulose, xylan, chitin and pectin substrates (except for low/no pectinase activity by the Micromonosporaceae)...
January 13, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28075554/an-engineered-allele-of-afsq1-facilitates-the-discovery-and-investigation-of-cryptic-natural-products
#16
Martin Daniel-Ivad, Nabeela Hameed, Stephanie Tan, Rachna Dhanjal, Daniel Socko, Patricia Pak, Tomas Gverzdys, Marie A Elliot, Justin R Nodwell
New approaches to antimicrobial discovery are needed to address the growing threat of antibiotic resistance. The Streptomyces genus, a proven source of antibiotics, is recognized as having a large reservoir of untapped secondary metabolic genes, many of which are likely to produce uncharacterized compounds. However, most of these compounds are currently inaccessible, as they are not expressed under standard laboratory conditions. Here we present a novel methodology for activating these 'cryptic' metabolites by heterologously expressing a constitutively active pleiotropic regulator...
January 11, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28074848/crystal-structure-of-stna-for-the-biosynthesis-of-antitumor-drug-streptonigrin-reveals-a-unique-substrate-binding-mode
#17
Tianle Qian, Jing Wo, Yan Zhang, Quanwei Song, Guoqiang Feng, Ray Luo, Shuangjin Lin, Geng Wu, Hai-Feng Chen
Streptonigrin methylesterase A (StnA) is one of the tailoring enzymes that modify the aminoquinone skeleton in the biosynthesis pathway of Streptomyces species. Although StnA has no significant sequence homology with the reported α/β-fold hydrolases, it shows typical hydrolytic activity in vivo and in vitro. In order to reveal its functional characteristics, the crystal structures of the selenomethionine substituted StnA (SeMet-StnA) and the complex (S185A mutant) with its substrate were resolved to the resolution of 2...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28074371/microbial-flora-analysis-for-the-degradation-of-beta-cypermethrin
#18
Zhang Qi, Zhang Wei
In the Xinjiang region of Eurasia, sustained long-term and continuous cropping of cotton over a wide expanse of land is practiced, which requires application of high levels of pyrethroid and other classes of pesticides-resulting in high levels of pesticide residues in the soil. In this study, soil samples were collected from areas of long-term continuous cotton crops with the aim of obtaining microbial resources applicable for remediation of pyrethroid pesticide contamination suitable for the soil type and climate of that area...
January 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28074054/synergistic-antifungal-indolecarbazoles-from-streptomyces-sp-cns-42-associated-with-traditional-chinese-medicine-alisma-orientale
#19
Mei Liu, Pei Huang, Qian Wang, Biao Ren, Ayokunmi Oyeleye, Miaomiao Liu, Jingyu Zhang, Xiaolin Li, Xiaoping Zhang, Lixin Zhang, Xueting Liu
No abstract text is available yet for this article.
January 11, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28074048/elmenols-c-h-new-angucycline-derivatives-isolated-from-a-culture-of-streptomyces-sp-ifm-11490
#20
Yixizhuoma, Naoki Ishikawa, Mohamed S Abdelfattah, Masami Ishibashi
Chemical investigations of the ethyl acetate extract of Streptomyces sp. IFM 11490 have led to the isolation of six new angucycline metabolites, named elmenols C-H (1-6), along with the previously reported elmonin (7) and elmenols A (8) and B (9). The known LS1924A (10), 6-deoxy-8-methylrabelomycin (11), tetrangulol methyl ether (12) and angucyclinone (13) were additionally identified. The structures of the isolated compounds were elucidated by means of spectroscopic methods including UV, IR, HRESIMS, and 1D and 2D NMR...
January 11, 2017: Journal of Antibiotics
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