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Collagen and Elastin Studies

Katherine Y Liu, Jesse D Sengillo, Gabriel Velez, Ruben Jauregui, Lynn Y Sakai, Irene H Maumenee, Alexander G Bassuk, Vinit B Mahajan, Stephen H Tsang
BACKGROUND: SLIT2 is a protein ligand for the Roundabout (ROBO) receptor and was found to play a major role in repulsive midline axon guidance in central nervous system development. Based on studies utilizing knockout models, it has been postulated that SLIT2 is important for preventing inappropriate axonal routing during mammalian optic chiasm development. METHODS: Case report. RESULTS: Here, we report a case of congenital myopia, anisometropia, and obesity in a patient with a SLIT2 point mutation...
August 15, 2018: Orphanet Journal of Rare Diseases
Mingmei Meng, Chunxiao Lv, Qingqing Yang, Shushu He, Shu Wu, Yi Liu, Jing Zou, Xuedong Zhou, Song Chen
INTRODUCTION: The aims of this study were to investigate the expression of proteins of elastic fibers and collagen type I in the supra-alveolar structure of orthodontically rotated teeth in rats and to elucidate whether circumferential supracrestal fiberotomy diminishes relapse. METHODS: The rats' maxillary left first molars were rotated by couple of force. Specimens were divided into groups according to different orthodontic procedures. A1-3 and B1-3 were blank control groups and operation control groups...
August 2018: American Journal of Orthodontics and Dentofacial Orthopedics
Bian Wu, Evan C Werlin, Mian Chen, Giorgio Mottola, Anuran Chatterjee, Kevin D Lance, Daniel A Bernards, Brian E Sansbury, Matthew Spite, Tejal A Desai, Michael S Conte
OBJECTIVE: Inflammation is a key driver of excessive neointimal hyperplasia within vein grafts. Recent work demonstrates that specialized proresolving lipid mediators biosynthesized from omega-3 polyunsaturated fatty acids, such as resolvin D1 (RvD1), actively orchestrate the process of inflammation resolution. We investigated the effects of local perivascular delivery of RvD1 in a rabbit vein graft model. METHODS: Ipsilateral jugular veins were implanted as carotid interposition grafts through an anastomotic cuff technique in New Zealand white rabbits (3-4 kg; N = 80)...
July 28, 2018: Journal of Vascular Surgery
Eleanor M Feneck, Philip N Lewis, Jim Ralphs, Keith M Meek
The cornea relies on its organised extracellular matrix for maintaining transparency and biomechanical strength. Studies have identified an elastic fibre system within the human posterior cornea, thought to allow for slight deformations in response to internal pressure fluctuations within the eye. However, the type of elastic fibres that exist within the cornea and their roles remain elusive. The aim of this study was to compare the distribution and organisation of the elastic fibres within the posterior peripheral mouse and human cornea, and elucidate how these fibres integrate with the trabecular meshwork, whilst characterising the distribution of their main likely components (fibrillin-1, elastin and type VI collagen) in different parts of the cornea and adjacent sclera...
July 25, 2018: Experimental Eye Research
Hiroaki Kasashima, Masakazu Yashiro, Tomohisa Okuno, Yuichiro Miki, Kisyu Kitayama, Go Masuda, Haruhito Kinoshita, Tamami Morisaki, Tatsunari Fukuoka, Tsuyoshi Hasegawa, Katsunobu Sakurai, Takahiro Toyokawa, Naoshi Kubo, Hiroaki Tanaka, Kazuya Muguruma, Kosei Hirakawa, Masaichi Ohira
BACKGROUND/AIMS: Lysyl oxidase (LOX) family members play a key role in modifying the primary tumor microenvironment by crosslinking collagens and elastin in the extracellular matrix. The aim of this study was to analyze the LOX-like (LOXL)1, LOXL3, and LOXL4 expressions in gastric cancer tissue by immunohistochemical staining. METHODS: The correlations between the clinicopathological features of 597 primary gastric carcinomas and LOX family members - LOXL1, LOXL3, and LOXL4 - were investigated by immunohistochemical studies...
July 25, 2018: Digestion
Daniele Pezzoli, Joseph Di Paolo, Heena Kumra, Giulia Fois, Gabriele Candiani, Dieter P Reinhardt, Diego Mantovani
One of the tightest bottlenecks in vascular tissue engineering (vTE) is the lack of strength and elasticity of engineered vascular wall models caused by limited elastic fiber deposition. In this study, flat and tubular collagen gel-based scaffolds were cellularised with vascular smooth muscle cells (SMCs) and supplemented with human plasma fibronectin (FN), a known master organizer of several extracellular matrix (ECM) fiber systems. The consequences of FN on construct maturation was investigated in terms of geometrical contraction, viscoelastic mechanical properties and deposition of core elastic fiber proteins...
October 2018: Biomaterials
Giampaolo Martufi, Arianna Forneris, Samaneh Nobakht, Kristina D Rinker, Randy D Moore, Elena S Di Martino
Introduction: Current recommendations for surgical treatment of abdominal aortic aneurysms (AAAs) rely on the assessment of aortic diameter as a marker for risk of rupture. The use of aortic size alone may overlook the role that vessel heterogeneity plays in aneurysmal progression and rupture risk. The aim of the current study was to investigate intra-patient heterogeneity of mechanical and fluid mechanical stresses on the aortic wall and wall tissue histopathology from tissue collected at the time of surgical repair...
2018: Frontiers in Cardiovascular Medicine
Suzanne Edgar, Blake Hopley, Licia Genovese, Sara Sibilla, David Laight, Janis Shute
Nutraceuticals containing collagen peptides, vitamins, minerals and antioxidants are innovative functional food supplements that have been clinically shown to have positive effects on skin hydration and elasticity in vivo. In this study, we investigated the interactions between collagen peptides (0.3-8 kDa) and other constituents present in liquid collagen-based nutraceuticals on normal primary dermal fibroblast function in a novel, physiologically relevant, cell culture model crowded with macromolecular dextran sulphate...
July 11, 2018: Scientific Reports
P de Graaf, R Ramadan, E C Linssen, N A Staller, A P A Hendrickx, G L S Pigot, E J H Meuleman, M Bouman, M Özer, J L H R Bosch, L M O de Kort
PURPOSE: Urethral reconstruction is performed in patients with urethral strictures or for correction of congenital disorders. In the case of shortage of tissue, engineered tissue may enhance urethral reconstruction. As the corpus spongiosum (CS) is important in supporting the function of the urethra, tissue engineering of the urethra should be combined with reconstruction of a CS. For that purpose, detailed knowledge of the composition of the CS, more specifically its extracellular matrix (ECM) and vascularization is needed for scaffold design...
July 9, 2018: Histology and Histopathology
Qian Liu, Yaxi Zhu, Peter C Amadio, Steven L Moran, Anne Gingery, Chunfeng Zhao
Tendon injuries are among the most common and severe hand injuries with a high demand for functional recovery. Stem cells have been identified and isolated from different species and a variety of tissues for the sake of regenerative medicine. Recently, turkey has been suggested as a potential new large animal model for flexor tendon-related research. However, turkey tissue-specific stem cells have not been investigated. Here, we presented the isolation and verification of tendon-derived stem cells (TDSCs) from 6- to 8-month-old heritage-breed turkey...
2018: Stem Cells International
Elise B Nguyen, Jeremy Wishner, Katarzyna Slowinska
Electrical stimulation of tissues has many uses in pain management, antibacterial treatment, and wound healing. The electric field stimulates epidermal migration and increases fibroblast cell proliferation. Here we show the effects of electrical field (EF) stimulation of human dermal fibroblasts (HDF) on the expression of collagen, elastin, and collagenase (MMP1; matrix metalloproteinase 1). The effects of EF stimulation are evaluated in terms of changes in cell morphology and extracellular matrix (ECM) protein expression, defined as intracellular concentration of collagen, elastin, and MMP1...
March 1, 2018: Journal of Electroanalytical Chemistry
Rezar P Deveja, Dimitrios C Iliopoulos, Eleftherios P Kritharis, Dimitrios C Angouras, Dimitrios Sfyris, Stavroula A Papadodima, Dimitrios P Sokolis
BACKGROUND: Previous studies have not examined the participation of intimal, medial, and adventitial layers in providing mechanical strength to the ascending thoracic aortic aneurysm (ATAA) wall compared to the non-aneurysmal aorta. A comparison is made herein of the mechanical properties of intact wall and its layers among ATAA and non-aneurysmal aorta, with explicit consideration of the effects of valve morphology, i.e. bicuspid (BAV) vs. tricuspid aortic valve (TAV), and aortic quadrant...
June 28, 2018: Annals of Thoracic Surgery
Felke Steijns, Jolanda van Hengel, Patrick Sips, Julie De Backer, Marjolijn Renard
Fibrillins are major constituents of microfibrils, which are essential components of the extracellular matrix of connective tissues where they contribute to the tissue homeostasis. Although it is known that microfibrils are abundantly expressed in the left ventricle of the heart, limited data are available about the presence of microfibrils in the other parts of the myocardial tissue and whether there are age or sex-related differences in the spatial arrangement of the microfibrils. This basic knowledge is essential to better understand the impact of fibrillin-1 pathogenic variants on the myocardial tissue as seen in Marfan related cardiomyopathy...
June 20, 2018: Histochemistry and Cell Biology
B De Servi, A Orlandini, E Caviola, M Meloni
The aim of this screening study was to evaluate the efficacy of different proprietary mixtures of amino acid and hyaluronic acid (HA) in stimulating the production of extracellular matrix (ECM) components, particularly the neo-synthesis of elastin, and in promoting a more efficient deposition of elastic fibres (elastogenesis), while at the same time maintaining the stimulation of collagen. The study has allowed identification of the optimal ratios between the amino acids (AA) for the production of collagen and elastin...
May 2018: Journal of Biological Regulators and Homeostatic Agents
Alexandra Schulz, Paul Christian Fuchs, Christian Oplaender, Leandra Börner Valdez, Jennifer Lynn Schiefer
Several reports have concluded that enzymatic debridement based on Bromelain (NX) is selective and efficient. Although clinical trials showed that viable tissue is not damaged at the macroscopic level, the effect on the cellular level is largely unknown. The current study is meant to close this gap by evaluating whether NX has an effect on vital cells of the human dermis on a cellular level. In an experimental in vitro study design, the effect of NX on human keratinocytes, fibroblasts, and macrophages was analyzed...
June 13, 2018: Journal of Burn Care & Research: Official Publication of the American Burn Association
George Lindesay, Yvonnick Bézie, Christophe Ragonnet, Véronique Duchatelle, Chandima Dharmasena, Nicole Villeneuve, Christine Vayssettes-Courchay
Central artery stiffening is recognized as a cardiovascular risk. The effects of hypertension and aging have been shown in human and animal models but the effect of salt is still controversial. We studied the effect of a high-salt diet on aortic stiffness in salt-sensitive spontaneously hypersensitive stroke-prone rats (SHRSP). Distensibility, distension, and β-stiffness were measured at thoracic and abdominal aortic sites in the same rats, using echotracking recording of the aortic diameter coupled with blood pressure (BP), in SHRSP-salt (5% salted diet, 5 weeks), SHRSP, and normotensive Wistar-Kyoto (WKY) rats...
2018: Journal of Vascular Research
Swarup S Swaminathan, Pedro Monsalve, Xiao Yi Zhou, Mabel Enriquez-Algeciras, Sanjoy K Bhattacharya, Sander R Dubovy, Anna K Junk
PURPOSE: To determine whether there are identifiable, reproducible findings in the trabecular meshwork (TM) of patients with primary open-angle glaucoma (POAG) who underwent Kahook Dual Blade (KDB) goniotomy. DESIGN: Noncomparative retrospective case series. METHODS: Tertiary academic referral center, Veterans Affairs Medical Center. Thirteen patients (14 eyes) with POAG (100%) were treated with KDB goniotomy from May to December 2017. Isolated TM tissue was collected from 9 patients (10 eyes) and submitted for histologic analysis...
August 2018: American Journal of Ophthalmology
Taisiya Bezhaeva, Margreet R de Vries, Wouter J Geelhoed, Eric P van der Veer, Sabine Versteeg, Carla M A van Alem, Bram M Voorzaat, Niels Eijkelkamp, Koen E van der Bogt, Alexander I Agoulnik, Anton-Jan van Zonneveld, Paul H A Quax, Joris I Rotmans
The pathophysiology of arteriovenous fistula (AVF) maturation failure is not completely understood but impaired outward remodeling (OR) and intimal hyperplasia are thought to be contributors. This adverse vascular response after AVF surgery results from interplay between vascular smooth muscle cells (VSMCs), the extracellular matrix (ECM), and inflammatory cells. Relaxin (RLN) is a hormone that acts on the vasculature via interaction with RLN/insulin-like peptide family receptor 1 (RXFP1), resulting in vasodilatation, ECM remodeling, and decreased inflammation...
June 8, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Monique C Suarez-Giron, Anabel Castro-Grattoni, Marta Torres, Ramon Farré, Ferran Barbé, Manuel Sánchez-de-la-Torre, David Gozal, Cesar Picado, Josep M Montserrat, Isaac Almendros
Study objectives: Chronic intermittent hypoxia (CIH), a hallmark feature of obstructive sleep apnea (OSA), induces accelerated atherogenesis as well as aorta vascular remodeling. Although the cyclooxygenase (COX) pathway has been proposed to contribute to the cardiovascular consequences of OSA, the potential benefits of a widely employed COX-inhibitor such (acetylsalicylic acid, ASA) on CIH-induced vascular pathology are unknown. Therefore, we hypothesized that a common non-selective COX inhibitor such as ASA would attenuate the aortic remodeling induced by CIH in mice...
2018: Frontiers in Physiology
Aurora Garre, Mridvika Narda, Palmira Valderas-Martinez, Jaime Piquero, Corinne Granger
Background: With age, decreasing dermal levels of proteoglycans, collagen, and elastin lead to the appearance of aged skin. Oxidation, largely driven by environmental factors, plays a central role. Aim: The aim of this study was to assess the antiaging efficacy of a topical serum containing L-Ascorbic acid, soluble proteoglycans, low molecular weight hyaluronic acid, and a tripeptide in ex vivo and in vivo clinical studies. Methods: Photoaging and photo-oxidative damage were induced in human skin explants by artificial solar radiation...
2018: Clinical, Cosmetic and Investigational Dermatology
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