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Collagen and Elastin Studies

Fabio Castiglione, Petter Hedlund, Emanuel Weyne, Lukman Hakim, Francesco Montorsi, Trinity J Bivalacqua, Dirk De Ridder, Uros Milenkovic, David Ralph, Giulio Garaffa, Asif Muneer, Steven Joniau, Maarten Albersen
INTRODUCTION: Previous studies have shown that the injection of adipose tissue-derived stem cells (ADSCs) into the tunica albuginea (TA) during the active phase of Peyronie's disease (PD) prevents the development of fibrosis. AIM: To investigate, using an animal model, whether local injection of human ADSCs (hADSCs) can alter the degree of fibrosis in the chronic phase of PD. METHODS: 27 male, 12-week-old rats were divided into 3 equal groups: sham, PD without treatment, and PD treated with hADSCs 1 month after disease induction...
November 29, 2018: Sexual Medicine
Yoon J Lee, Seung E Baek, Sujin Lee, Yong W Cho, Yeon J Jeong, Ki J Kim, Young J Jun, Jong W Rhie
The potential use of extracellular matrix (ECM) as a source of wound dressing material has recently received much attention. The ECM is an intricate network of various combinations of elastin, collagens, laminin, fibronectin, and proteoglycans that play a key role in stimulating cell proliferation and differentiation. We evaluated the efficacy of an ECM sheet derived from human adipose tissue as a wound dressing material to enhance healing. We prepared a novel porous ECM sheet dressing scaffold from human adipose tissue...
November 20, 2018: International Wound Journal
K Sivaraman, C Shanthi
Matrikines, peptides originating from the fragmentation of extracellular matrix proteins are identified to play important role in both health and disease. They possess biological activities, much different from their parent protein. Identification of such bioactive cryptic regions in the extracellular matrix proteins has attracted the researchers all over the world in the recent decade. These bioactive peptides could find use in preparation of biomaterials and tissue engineering applications. Matrikines identified in major extracellular matrix (ECM) proteins like collagen, elastin, fibronectin, and laminin are being extensively studied for use in tissue engineering and regenerative medicine...
December 1, 2018: Life Sciences
Elisa Zambaiti, Federico Scottoni, Eleonora Rizzi, Simone Russo, Koichi Deguchi, Simon Eaton, Alessandro F Pellegata, Paolo De Coppi
BACKGROUND: Conditions leading to reduced gastric volume are difficult to manage and are associated to poor quality-of-life. Stomach augmentation using a tissue-engineered stomach is a potential solution to restore adequate physiology and food reservoir. Aim of this study was to evaluate the decellularisation of whole rat stomach using a detergent-enzymatic protocol. METHODS: Stomachs harvested from rats were decellularised through luminal and vascular cannulation using 24-h detergent-enzymatic treatment and completely characterized by appropriate staining, DNA and Extracellular matrix -component quantifications...
November 16, 2018: Pediatric Surgery International
Yutaka Yasui, Tokiya Abe, Masayuki Kurosaki, Kotaro Matsunaga, Mayu Higuchi, Nobuharu Tamaki, Keiya Watakabe, Mao Okada, Wan Wang, Takao Shimizu, Kenta Takaura, Yohei Masugi, Hiroyuki Nakanishi, Kaoru Tsuchiya, Yuka Takahashi, Jun Itakura, Urara Sakurai, Akinori Hashiguchi, Michiie Sakamoto, Namiki Izumi
BACKGROUND AND AIMS: Elastic fiber deposition is a cause of irreversibility of liver fibrosis. However, to date, its relevance to clinical features has not yet been clarified. This study aimed to clarify the correlation between non-invasive markers of fibrosis and fiber quantity, including elastic fiber, obtained from computational analysis. METHODS: This retrospective study included 270 patients evaluated by non-invasive liver fibrosis assessment prior to liver biopsy...
November 12, 2018: Hepatology Research: the Official Journal of the Japan Society of Hepatology
Jeffrey M Mattson, Yunjie Wang, Yanhang Zhang
The contribution of glycosaminoglycans (GAGs) to the biological and mechanical functions of biological tissue has emerged as an important area of research. GAGs provide structural basis for the organization and assembly of extracellular matrix (ECM). The mechanics of tissue with low GAG content can be indirectly affected by the interaction of GAGs with collagen fibers, which have long been known to be one of the primary contributors to soft tissue mechanics. Our earlier study showed that enzymatic GAG depletion results in straighter collagen fibers that are recruited at lower levels of stretch, and a corresponding shift in earlier arterial stiffening (Mattson et al...
November 1, 2018: Journal of Biomechanics
Thuy-Uyen Nguyen, Mozhgan Shojaee, Chris A Bashur, Vipuil Kishore
Biomimetic tissue-engineered vascular grafts (TEVGs) have immense potential to replace diseased small-diameter arteries (<4 mm) for the treatment of cardiovascular diseases. However, biomimetic approaches developed thus far only partially recapitulate the physicochemical properties of the native vessel. While it is feasible to fabricate scaffolds that are compositionally similar to native vessels (collagen and insoluble elastic matrix) using freeze-drying, these scaffolds do not mimic the aligned topography of collagen and elastic fibers found in native vessels...
November 9, 2018: Biofabrication
Shier Nee Saw, Jess Jia Hwee Tay, Yu Wei Poh, Liying Yang, Wei Ching Tan, Lay Kok Tan, Alys Clark, Arijit Biswas, Citra Nurfarah Zaini Mattar, Choon Hwai Yap
Intrauterine growth restriction (IUGR) is a pregnancy complication due to placental dysfunction that prevents the fetus from obtaining enough oxygen and nutrients, leading to serious mortality and morbidity risks. There is no treatment for IUGR despite having a prevalence of 3% in developed countries, giving rise to an urgency to improve our understanding of the disease. Applying biomechanics investigation on IUGR placental tissues can give important new insights. We performed pressure-diameter mechanical testing of placental chorionic arteries and found that in severe IUGR cases (RI > 90th centile) but not in IUGR cases (RI < 90th centile), vascular distensibility was significantly increased from normal...
November 8, 2018: Scientific Reports
Yuki Sato, Rena Watanabe, Nozomi Uchiyama, Nana Ozawa, Yui Takahashi, Remina Shirai, Kengo Sato, Yusaku Mori, Taka-Aki Matsuyama, Hatsue Ishibashi-Ueda, Tsutomu Hirano, Takuya Watanabe
Vasostatin-1,a chromogranin A-derived peptide (76 amino acids),is known to suppress vasoconstriction and angiogenesis.A recent study has shown that vasostatin-1 suppresses the adhesion of human U937 monocytes to human endothelial cells (HECs) via adhesion molecule downregulation. The present study evaluated the expression of vasostatin-1 in human atherosclerotic lesionsand its effects on inflammatory responses in HECs and human THP-1 monocyte-derived macrophages,macrophagefoam cell formation, migration and proliferation of human aortic smooth muscle cells (HASMCs) and extracellular matrix production by HASMCs, and atherogenesisin apolipoprotein E-deficient (ApoE-/- ) mice...
November 6, 2018: Clinical Science (1979-)
Pablo González Isaza
Vulvo-vaginal atrophy as a main symptom of the Genito Urinary Syndrome of Menopause (GSM) is a consequence of aging, particularly after menopause as a result of follicular ovarian follicle depletion and consequently low estrogen levels. Anatomical structures derived from the urogenital sinus, such as the distal urethra trigone and vestibule, are the most affected areas because of the high concentrations of alfa- and beta-estrogen receptors. The most common symptoms associated with vulvo-vaginal atrophy are dyspareunia, vaginal dryness, irritation, recurrent urinary tract infection and urinary incontinence, which negatively affect the patient's quality of life and sexuality...
November 3, 2018: Surgical Technology International
Olivier G de Jong, Lizet M van der Waals, Farah R W Kools, Marianne C Verhaar, Bas W M van Balkom
Lysyl oxidase-like 2 (LOXL2) belongs to the family of lysyl oxidases, and as such promotes crosslinking of collagens and elastin by oxidative deamination of lysine residues. In endothelial cells (ECs), LOXL2 is involved in crosslinking and scaffolding of collagen IV. Additionally, several reports have shown a role for LOXL2 in other processes, including regulation of gene expression, tumor metastasis, and epithelial-to-mesenchymal transition (EMT). Here, we demonstrate an additional role for LOXL2 in the regulation of angiogenesis by modulation of endothelial-to-mesenchymal transition (EndMT)...
November 1, 2018: Journal of Cellular Physiology
Carlos Gasanz, Carles Raventós, Jordi Temprana-Salvador, Marielle Esteves, Carla Fonseca, Inés de Torres, Juan Morote
Introduction: Bladder reconstruction without using the intestine remains a challenge to this day despite the development of new biomaterials and cell cultures. Human bladder engineering is merely anecdotic, and mostly in vitro and animal studies have been conducted. Material and methods: In our study using a porcine model, we performed a bladder augmentation using an autologous parietal peritoneum graft (peritoneocystoplasty) and determined whether the attachment of an acellular collagen-elastin matrix (Group 1) or lack of (Group 2) had better histologic and functional results...
2018: Central European Journal of Urology
Jasmin Imsirovic, Erzsébet Bartolák-Suki, Samer Bou Jawde, Harikrishnan Parameswaran, Béla Suki
Vascular smooth muscle cells respond to mechanical stretch by reorganizing their cytoskeletal and contractile elements. Recently, we showed that contractile forces in rat aorta rings were maintained when the rings were exposed to 4 h of physiological variability in cycle-by-cycle strain, called variable stretch (VS), mimicking beat-to-beat blood pressure variability. Contractility, however, was reduced when the aorta was exposed to monotonous stretch (MS) with an amplitude equal to the mean peak strain of VS...
October 30, 2018: Physiological Measurement
Manying Wang, Xuenan Chen, Wenqi Jin, Xiaohao Xu, Xiangyan Li, Liwei Sun
Cigarette smoke causes many adverse effects such as inflammation, oxidative stress, and excessive accumulation of the extracellular matrix (ECM). Ginsenoside Rb3 has anti-inflammatory and anti-oxidative effects, which may contribute to delaying the injury caused by cigarette smoke. In this study, we used cigarette smoke extract (CSE) to establish cell injury models in WI-38 human fetal lung fibroblasts and 16HBE human bronchial epithelial cells. Our results showed that Rb3 protected against CSE-induced cytotoxicity in both cell lines...
December 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Kamalika Mukherjee, Chi Young Song, Anne M Estes, Ahmad N Dhodi, Benjamin H Ormseth, Ji Soo Shin, Frank J Gonzalez, Kafait U Malik
Background We have reported that cytochrome P450 1B1 ( CYP 1B1), expressed in cardiovascular tissues, contributes to angiotensin II -induced vascular smooth muscle cell ( VSMC ) migration and proliferation and development of hypertension in various experimental animal models via generation of reactive oxygen species. This study was conducted to determine the contribution of CYP 1B1 to platelet-derived growth factor-BB-induced VSMC migration and proliferation in vitro and to neointimal growth in vivo. Methods and Results VSMC s isolated from aortas of male Cyp1b1 +/+ and Cyp1b1 -/- mice were used for in vitro experiments...
September 18, 2018: Journal of the American Heart Association
Yanhong Li, Chunxiao Zhang, Fengxiang Luo, Biyu Peng
Leather biotechnology based on enzyme is one of the main directions toward clean technology in the leather manufacturing process. Proteins such as collagen, elastin, and keratin are important components in animal hides or skins, and proteases are most frequently used in the leather manufacturing process for the removal of interfibrillar substance and opening-up of collagen fiber instead of toxic chemicals. Elastin is an important and highly elastic structural protein in the animal hides or skins and significantly affects the properties of the final leather product...
October 27, 2018: Applied Microbiology and Biotechnology
Rosanna Avola, Adriana Carol Eleonora Graziano, Giovanna Pannuzzo, Francesco Bonina, Venera Cardile
Skin aging is a complex biological process influenced by a combination of endogenous or intrinsic and exogenous or extrinsic factors due to environmental damage. The primary environmental factor that causes human skin aging is the ultraviolet irradiation from the sun. Recently, it was established that the long-term exposure to light-emitting-diode-generated blue light (LED-BL) from electronic devices seems to have a relevant implication in the molecular mechanisms of premature photoaging. BL irradiation induces changes in the synthesis of various skin structures through DNA damage and overproduction of reactive oxygen species (ROS), matrix metalloproteinase-1 and -12, which are responsible for the loss of the main components of the extracellular matrix of skin like collagen type I and elastin...
October 26, 2018: Journal of Cellular Physiology
Benjamin P Cohen, Jaime L Bernstein, Kerry A Morrison, Jason A Spector, Lawrence J Bonassar
Children suffering from microtia have few options for auricular reconstruction. Tissue engineering approaches attempt to replicate the complex anatomy and structure of the ear with autologous cartilage but have been limited by access to clinically accessible cell sources. Here we present a full-scale, patient-based human ear generated by implantation of human auricular chondrocytes and human mesenchymal stem cells in a 1:1 ratio. Additional disc construct surrogates were generated with 1:0, 1:1, and 0:1 combinations of auricular chondrocytes and mesenchymal stem cells...
2018: PloS One
Alexander Rachev, Tarek Shazly
The study proposes a novel theoretical-experimental approach for structure-based constitutive modeling of the passive mechanical properties of arterial tissue. The major novelty is accounting for the existence of individual natural configurations of elastin and collagen and their mechanical interaction in terms of the constituents' individual prestretches in the tissue natural state. The structure-based modeling of collagen allows accounting for effects of change in constituents' prestretch in terms of the change in feasible microstructural parameters, such as range of collagen recruitment stretch, mode of collagen mass fraction intensity function, and fiber directions...
October 2, 2018: Journal of the Mechanical Behavior of Biomedical Materials
Hye Shin Lee, Jinhyeok Choi, Taekwon Son, Eun Ji Lee, Jeong-Gyun Kim, Soo Hyung Ryu, Danbi Lee, Myoung Kuk Jang, Eunsil Yu, Young-Hwa Chung, Irwin H Gelman, Kyu-Won Kim
AKAP12 belongs to A-kinase anchoring protein (AKAP) family of scaffold proteins and is known as a tumor suppressor in several human cancer types. Its role as a tumor suppressor in hepatocellular carcinoma (HCC) was proposed due to its downregulation and epigenetic modification in human HCC; however, the effect of its deficiency on liver injuries, such as liver fibrosis and cancer has been poorly studied. By analyzing tumor and non-tumor tissues of 15 patients with HCC, it was confirmed that AKAP12 expression was downregulated in human HCC as compared with adjacent non-tumor tissues...
November 2018: Oncology Letters
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