keyword
https://read.qxmd.com/read/38054720/val43-residue-of-nsrr-is-crucial-for-the-nitric-oxide-response-of-salmonella-typhimurium
#21
JOURNAL ARTICLE
Hee Jeong Park, Hye Won Jeong, Choa Lee, Mi Rae Lee, Hojung Choi, Eungseok Kim, Iel Soo Bang
The precise regulation of flavohemoglobin Hmp expression by NsrR is critical for bacterial fitness, as excessive Hmp expression in the absence of NO can disturb bacterial redox homeostasis. While the molecular structure of Streptomyces coelicolor NsrR has been recently identified, the specific molecular structures of NsrR proteins in enterobacteria remain unknown. Our discovery of the crucial role of Val43 in the DNA recognition helix α3 of Salmonella NsrR offers valuable insights into the Hmp modulation under NO stress...
December 6, 2023: Microbiology Spectrum
https://read.qxmd.com/read/38038757/high-intensity-green-light-potentially-activates-the-actinorhodin-biosynthetic-pathway-in-streptomyces-coelicolor-a3-2
#22
JOURNAL ARTICLE
Chompoonik Kanchanabanca, Takeshi Hosaka, Masanobu Kojima
The development of practices that enhance the potential of actinomycetes as major antibiotic producers is a challenge in discovering new secondary metabolites. Light, an essential external stimulus for most microorganisms, could be exploited to manipulate their physiological processes. However, the effects of monochromatic green light on the production of secondary metabolites in actinomycetes have not yet been reported. In this paper, we report a novel and simple method that uses high-intensity monochromatic green light to potentially induce the production of cryptic secondary metabolites in the model actinomycete Streptomyces coelicolor A3(2)...
December 1, 2023: Archives of Microbiology
https://read.qxmd.com/read/38029966/characterization-of-the-cystargolide-biosynthetic-gene-cluster-and-functional-analysis-of-the-methyltransferase-cysg
#23
JOURNAL ARTICLE
Patrick Beller, Phillipp Fink, Felix Wolf, Daniel Männle, Irina Helmle, Wolfgang Kuttenlochner, Daniel Unterfrauner, Alicia Engelbrecht, Nicole D Staudt, Andreas Kulik, Michael Groll, Harald Gross, Leonard Kaysser
Cystargolides are natural products originally isolated from Kitasatospora cystarginea NRRL B16505 as inhibitors of the proteasome. They are composed of a dipeptide backbone linked to a β-lactone warhead. Recently, we identified the cystargolide biosynthetic gene cluster but systematic genetic analyses had not been carried out because of the lack of a heterologous expression system. Here, we report the discovery of a homologous cystargolide biosynthetic pathway in Streptomyces durhamensis NRRL-B3309 by genome mining...
November 27, 2023: Journal of Biological Chemistry
https://read.qxmd.com/read/38020227/cloning-sequencing-and-characterizing-of-soil-antibiotic-active-producing-streptomyces-species-specific-dna-markers
#24
JOURNAL ARTICLE
Ismail Saadoun, Amjad Mahasneh, Jazi D Odat, Ban Al-Joubori, Elsiddig Elsheikh
Association of the antibiotic activity of the soil Streptomyces isolates to their genetic profiles analyzed through RAPD-PCR fingerprints prompted us here in this study to use the most common bands as specific markers to identify homologous proteins within these isolates by cloning, sequencing, and characterizing these markers. Six out of twelve DNA bands ranged between 600 and 1350 bp previously obtained by RAPD-PCR analysis were purified out of the RAPD gels, and then cloned into pGEM-T Easy vector system...
December 2023: Saudi Journal of Biological Sciences
https://read.qxmd.com/read/38013192/-characterization-of-a-d-mannitol-oxidase-from-paenibacillus-sp-and-its-application-in-the-preparation-of-d-mannose
#25
JOURNAL ARTICLE
Ran Li, Cong Song, Xiang Zhang, Zhenhua Jia
D-mannose has many functional activities and is widely used in food, medicine, agriculture and other industries. D-mannitol oxidase that can efficiently convert D-mannitol into D-mannose has potential application in the enzymatic preparation of D-mannose. A D-mannitol oxidase (PsOX) was found from Paenibacillus sp. HGF5. The similarity between PsOX and the D-mannitol oxidase (AldO) from Streptomyces coelicolor was 50.94%. The molecular weight of PsOX was about 47.4 kDa. A recombinant expression plasmid pET-28a-PsOX was constructed and expressed in Escherichia coli BL21(DE3)...
November 25, 2023: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://read.qxmd.com/read/37989784/co-factor-independent-oxidases-ncnn-and-actva-3-are-involved-in-the-dimerization-of-benzoisochromanequinone-antibiotics-in-naphthocyclinone-and-actinorhodin-biosynthesis
#26
JOURNAL ARTICLE
Bikash Baral, Soheila Matroodi, Vilja Siitonen, Keshav Thapa, Amir Akhgari, Keith Yamada, Aleksi Nuutila, Mikko Metsä-Ketelä
Streptomyces produce complex bioactive secondary metabolites with remarkable chemical diversity. Benzoisochromanequinone polyketides actinorhodin and naphthocyclinone are formed through dimerization of half-molecules via single or double carbon-carbon bonds, respectively. Here we sequenced the genome of S. arenae DSM40737 to identify the naphthocyclinone gene cluster and established heterologous production in S. albus J1074 by utilizing direct cluster capture techniques. Comparative sequence analysis uncovered ncnN and ncnM gene products as putative enzymes responsible for dimerization...
November 21, 2023: FEMS Microbiology Letters
https://read.qxmd.com/read/37986689/streptomyces-global-regulators-afsr-and-afss-interact-to-co-regulate-antibiotic-production-and-morphological-development
#27
JOURNAL ARTICLE
Yi Hao, Wenshuai Liu, Xingwang Li, Ying Wen
Streptomyces species have a complex life cycle and are the producers of ~70% of commercial antibiotics. Global regulators AfsR and AfsS are widespread among Streptomyces and have been identified as key activators of antibiotic production in several species. However, their roles as repressors of antibiotic production are unclear; in particular, nothing is known regarding the regulatory mechanism of AfsS, despite many decades of research, because it has no DNA-binding domain. Here, we demonstrate that AfsR and AfsS negatively regulate avermectin production and morphological development in the industrially important species S...
November 20, 2023: Microbial Biotechnology
https://read.qxmd.com/read/37983668/biosynthesis-of-4-acyl-5-aminoimidazole-alkaloids-featuring-a-new-friedel-crafts-acyltransferase
#28
JOURNAL ARTICLE
Yuwei Xia, Guoliang Zhu, Xingwang Zhang, Shengying Li, Lei Du, Weiming Zhu
Friedel-Crafts acylation (FCA) is a highly beneficial approach in organic chemistry for creating the important C-C bonds that are necessary for building intricate frameworks between aromatic substrates and an acyl group. However, there are few reports about enzyme catalyzed FCA reactions. In this study, 4-acyl-5-aminoimidazole alkaloids (AAIAs), streptimidazoles A-C ( 1 - 3 ), and the enantiopure (+)-nocarimidazole C ( 4 ) as well as their ribosides, streptimidazolesides A-D ( 5 - 8 ), were identified from the fermentation broth of Streptomyces sp...
November 20, 2023: Journal of the American Chemical Society
https://read.qxmd.com/read/37982622/systems-wide-analysis-of-the-rok-family-regulatory-gene-rokl6-and-its-role-in-the-control-of-glucosamine-toxicity-in-streptomyces-coelicolor
#29
JOURNAL ARTICLE
Chao Li, Mia Urem, Chao Du, Le Zhang, Gilles P van Wezel
Central metabolism plays a key role in the control of growth and antibiotic production in streptomycetes. Specifically, aminosugars act as signaling molecules that affect development and antibiotic production, via metabolic interference with the global repressor DasR. While aminosugar metabolism directly connects to other major metabolic routes such as glycolysis and cell wall synthesis, several important aspects of their metabolism are yet unresolved. Accumulation of N -acetylglucosamine 6-phosphate or glucosamine 6-phosphate is lethal to many bacteria, a yet unresolved phenomenon referred to as "aminosugar sensitivity...
November 20, 2023: Applied and Environmental Microbiology
https://read.qxmd.com/read/37978256/genome-sequence-and-characterization-of-streptomyces-phages-vanseggelen-and-verabelle-representing-two-new-species-within-the-genus-camvirus
#30
JOURNAL ARTICLE
Véronique Ongenae, Annabel Kempff, Vera van Neer, Helena Shomar, Florian Tesson, Daniël Rozen, Ariane Briegel, Dennis Claessen
Despite the rising interest in bacteriophages, little is known about their infection cycle and lifestyle in a multicellular host. Even in the model system Streptomyces, only a small number of phages have been sequenced and well characterized so far. Here, we report the complete characterization and genome sequences of Streptomyces phages Vanseggelen and Verabelle isolated using Streptomyces coelicolor as a host. A wide range of Streptomyces strains could be infected by both phages, but neither of the two phages was able to infect members of the closely related sister genus Kitasatospora...
November 17, 2023: Scientific Reports
https://read.qxmd.com/read/37974397/the-influence-of-coiled-coil-motif-of-serine-recombinase-toward-the-directionality-regulation
#31
JOURNAL ARTICLE
Yei-Wei Chen, Bo-Yu Su, Gregory D Van Duyne, Paul Fogg, Hsiu-Fang Fan
Serine integrases promote the recombination of two complementary DNA sequences, attP and attB, to create hybrid sequences, attL and attR. The reaction is unidirectional in the absence of an accessory protein called recombination directionality factor (RDF). We utilized Tethered Particle Motion (TPM) experiments to investigate the reaction behaviours of two model serine integrases from Listeria innocua phage LI and Streptomyces coelicolor phage C31. Detailed kinetic analyses of wild-type and mutant proteins were carried out to verify the mechanisms of recombination directionality...
November 16, 2023: Biophysical Journal
https://read.qxmd.com/read/37951404/single-cell-icp-ms-to-evaluate-the-interaction-behaviour-for-cd-ce-and-u-with-streptomyces-coelicolor-spores
#32
JOURNAL ARTICLE
Steffen Hellmann, Paula García-Cancela, Sergio Alonso-Fernández, Mario Corte-Rodríguez, Jörg Bettmer, Angel Manteca, Dirk Merten, Teba Gil-Díaz, Thorsten Schäfer, María Montes-Bayón
Streptomyces are important soil bacteria used for bioremediation of metal-contaminated soils, however, it is still unknown how metal-selective Streptomyces are and which mechanisms are involved during their capture. In this work, we exposed S. coelicolor spores to environmentally relevant concentrations (0.1, 1, 10, 100 μM) of Ce, U and Cd in solid medium for one week to investigate the uptake behaviour of hyphae in the newly formed spores. Additionally, metal adsorption onto the spores was explored by incubating inactive, ungerminated spores for one day in aqueous metal solution...
November 9, 2023: Chemosphere
https://read.qxmd.com/read/37926680/increasing-reduction-potentials-of-type-1-copper-center-and-catalytic-efficiency-of-small-laccase-from-streptomyces-coelicolor-through-secondary-coordination-sphere-mutations
#33
JOURNAL ARTICLE
Jing-Xiang Wang, Avery C Vilbert, Chang Cui, Evan N Mirts, Lucas H Williams, Wantae Kim, Yan Jessie Zhang, Yi Lu
The key to type 1 copper (T1Cu) function lies in the fine tuning of the CuII/I reduction potential (E°´T1Cu) to match its redox partners, enabling efficient electron transfer in a wide range of biological systems. While the secondary coordination sphere (SCS) effects have been used to tune E°´T1Cu in azurin over a wide range, these principles are yet to be generalized to other T1Cu-containing proteins to tune catalytic properties. To this end, we have examined the effects of Y229F, V290N and S292F mutations around the T1Cu of small laccase (SLAC) from Streptomyces coelicolor to match the E°´T1Cu to high reduction potential fungal laccases...
November 5, 2023: Angewandte Chemie
https://read.qxmd.com/read/37923760/construction-of-streptomyces-coelicolor-a3-2-mutants-that-exclusively-produce-na4-na6-intermediates-of-agarose-metabolism-through-mutation-induction
#34
JOURNAL ARTICLE
Jina Kim, Eun Joo Kim, Hye-Jeong Ko, Yeon-Hee Lee, Soon-Kwang Hong, Miyoung Shin, Je Hyeon Lee, Woori Kwak
NA4/NA6, an intermediate degradation product of β-agarase, is a high value-added product with anticancer, anti-obesity, and anti-diabetic effects. Therefore, a method that enables the efficient production of NA4/NA6 would be useful from economic and medical perspectives. In this study, we aimed to generate a Streptomyces coelicolor A3(2) mutant M22-2C43 that produces NA4/NA6 as a final product; this method serves as a more efficient alternative to the enzymatic conversion of β-agarase for the generation of these products...
November 3, 2023: Scientific Reports
https://read.qxmd.com/read/37902333/the-plant-stress-hormone-jasmonic-acid-evokes-defensive-responses-in-streptomycetes
#35
JOURNAL ARTICLE
Anne van der Meij, Somayah S Elsayed, Chao Du, Joost Willemse, Thomas M Wood, Nathaniel I Martin, Jos M Raaijmakers, Gilles P van Wezel
Actinobacteria are prevalent in the rhizosphere and phyllosphere of diverse plant species where they help to enhance tolerance of plants against biotic and abiotic stresses. Here, we show that the plant hormones jasmonic acid (JA) and methyl jasmonate (MeJA) affect the growth, development, and specialized metabolism of Streptomyces . Exposure of Streptomyces coelicolor to JA or MeJA led to enhanced production of the polyketide antibiotic actinorhodin. JA also exhibited toxicity toward Streptomyces and Streptacidiphilus at higher concentrations, whereby streptomycetes were more tolerant to JA than members of the genus Streptacidiphilus ...
October 30, 2023: Applied and Environmental Microbiology
https://read.qxmd.com/read/37888469/multicopy-chromosome-integration-and-deletion-of-negative-global-regulators-significantly-increased-the-heterologous-production-of-aborycin-in-streptomyces-coelicolor
#36
JOURNAL ARTICLE
Jia-Yi Li, Jun-Yu Liang, Zhao-Yuan Liu, Yue-Zhao Yi, Jing Zhao, Zhi-Yong Huang, Jun Chen
Aborycin is a type I lasso peptide with a stable interlocked structure, offering a favorable framework for drug development. The aborycin biosynthetic gene cluster gul from marine sponge-associated Streptomyces sp. HNS054 was cloned and integrated into the chromosome of S. coelicolor hosts with different copies. The three-copy gul -integration strain S. coelicolor M1346::3 gul showed superior production compared to the one-copy or two-copy gul -integration strains, and the total titer reached approximately 10...
October 13, 2023: Marine Drugs
https://read.qxmd.com/read/37876253/understanding-base-and-backbone-contributions-of-phosphorothioate-dna-for-molecular-recognition-with-sbd-proteins
#37
JOURNAL ARTICLE
Jiayi Li, Shenggan Luo, Xingyu Ouyang, Geng Wu, Zixin Deng, Xinyi He, Yi-Lei Zhao
Bacterial DNA phosphorothioate (PT) modification provides a specific anchoring site for sulfur-binding proteins (SBDs). Besides, their recognition patterns include phosphate links and bases neighboring the PT-modified site, thereby bringing about genome sequence-dependent properties in PT-related epigenetics. Here, we analyze the contributions of the DNA backbone (phosphates and deoxyribose) and bases bound with two SBD proteins in Streptomyces pristinaespiralis and coelicolor ( SBD Sco and SBD Spr ). The chalcogen-hydrophobic interactions remained constantly at the anchoring site while the adjacent bases formed conditional and distinctive non-covalent interactions...
October 25, 2023: Physical Chemistry Chemical Physics: PCCP
https://read.qxmd.com/read/37838667/heterologous-overproduction-of-oviedomycin-by-refactoring-biosynthetic-gene-cluster-and-metabolic-engineering-of-host-strain-streptomyces-coelicolor
#38
JOURNAL ARTICLE
Boncheol Gu, Duck Gyun Kim, Do-Kyung Kim, Minji Kim, Hyun Uk Kim, Min-Kyu Oh
BACKGROUND: Oviedomycin is one among several polyketides known for their potential as anticancer agents. The biosynthetic gene cluster (BGC) for oviedomycin is primarily found in Streptomyces antibioticus. However, because this BGC is usually inactive under normal laboratory conditions, it is necessary to employ systematic metabolic engineering methods, such as heterologous expression, refactoring of BGCs, and optimization of precursor biosynthesis, to allow efficient production of these compounds...
October 14, 2023: Microbial Cell Factories
https://read.qxmd.com/read/37799584/exploring-a-streptomyces-wax-synthase-using-acyl-snacs-as-donor-substrates
#39
JOURNAL ARTICLE
Federica Casolari, Saad Alrashdi, Reuben Carr, Hai Deng
The demand of fragrance and food industries for short/branched wax esters is increasing due to their rich scent and low toxicity. Wax synthase and acyl-CoA:diacylglycerol O -acyltransferase (WS/DGAT) are a family of bacterial enzymes capable of catalysing the production of wax esters. Here, we report that a WS/DGAT from Streptomyces coelicolor is able to mediate the reactions between alcohol acceptors and synthetic acyl-donor mimics, acyl-SNACs. The enzyme displayed considerable substrate tolerance towards acyl-donors with structural diversity...
October 4, 2023: RSC chemical biology
https://read.qxmd.com/read/37798648/mycothiol-maintains-the-homeostasis-and-signalling-of-nitric-oxide-in-streptomyces-coelicolor-a3-2-m145
#40
JOURNAL ARTICLE
Tomoki Yoshizumi, Yukiko Shibui, Minori Kogo, Sota Honma, Shinsaku Ito, Shunsuke Yajima, Yasuyuki Sasaki
BACKGROUND: Previous studies have revealed a nitric oxide (NO) metabolic cycle in which NO, nitrate (NO3 - ), and nitrite (NO2 - ) circulate. The NO produced in this cycle serves as a signalling molecule that regulates actinorhodin (ACT) production via the DevS/DevR NO-dependent two-component system (TCS) in Streptomyces coelicolor A3(2) M145. However, the mechanisms involved in the regulation of NO signalling in S. coelicolor have not yet been elucidated. Mycothiol (MSH), a thiol molecule produced by Actinomyces, is involved in the defence mechanisms against oxidative stress...
October 5, 2023: BMC Microbiology
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