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Streptomyces coelicolor

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https://www.readbyqxmd.com/read/28211492/the-propensity-of-the-bacterial-rodlin-protein-rdlb-to-form-amyloid-fibrils-determines-its-function-in-streptomyces-coelicolor
#1
Wen Yang, Joost Willemse, Elizabeth B Sawyer, Fei Lou, Weibin Gong, Hong Zhang, Sally L Gras, Dennis Claessen, Sarah Perrett
Streptomyces bacteria form reproductive aerial hyphae that are covered with a pattern of pairwise aligned fibrils called rodlets. The presence of the rodlet layer requires two homologous rodlin proteins, RdlA and RdlB, and the functional amyloid chaplin proteins, ChpA-H. In contrast to the redundancy shared among the eight chaplins, both RdlA and RdlB are indispensable for the establishment of this rodlet structure. By using a comprehensive biophysical approach combined with in vivo characterization we found that RdlB, but not RdlA, readily assembles into amyloid fibrils...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28210250/simultaneous-activation-of-iron-and-thiol-based-sensor-regulator-systems-by-redox-active-compounds
#2
Kang-Lok Lee, Ji-Sun Yoo, Gyeong-Seok Oh, Atul K Singh, Jung-Hye Roe
Bacteria in natural habitats are exposed to myriad redox-active compounds (RACs), which include producers of reactive oxygen species (ROS) and reactive electrophile species (RES) that alkylate or oxidize thiols. RACs can induce oxidative stress in cells and activate response pathways by modulating the activity of sensitive regulators. However, the effect of a certain compound on the cell has been investigated primarily with respect to a specific regulatory pathway. Since a single compound can exert multiple chemical effects in the cell, its effect can be better understood by time-course monitoring of multiple sensitive regulatory pathways that the compound induces...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28167521/ddar-pa1196-regulates-expression-of-dimethylarginine-dimethylaminohydrolase-for-the-metabolism-of-methylarginines-in-pseudomonas-aeruginosa-pao1
#3
Benjamin R Lundgren, Frank J Bailey, Gabriella Moley, Christopher T Nomura
: Dimethylarginine dimethylaminohydrolases or DDAHs catalyze the hydrolysis of methylarginines to yield L-citrulline and methylamines as products. DDAHs and their central roles in methylarginine metabolism have been characterized for eukaryotic cells. While DDAHs are known to exist in some bacteria, including Streptomyces coelicolor and Pseudomonas aeruginosa, the physiological importance and genetic regulation of bacterial DDAHs remain poorly understood. To provide some insight into bacterial methylarginine metabolism, this study focused on identifying the key elements or factors regulating DDAH expression in P...
February 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28112394/establishing-a-high-yielding-streptomyces-based-cell-free-protein-synthesis-system
#4
Jian Li, He Wang, Yong-Chan Kwon, Michael C Jewett
Cell-free protein synthesis (CFPS) has emerged as a powerful platform for applied biotechnology and synthetic biology, with a range of applications in synthesizing proteins, evolving proteins, and prototyping genetic circuits. To expand the current CFPS repertoire, we report here the development and optimization of a Streptomyces-based CFPS system for the expression of GC-rich genes. By developing a streamlined crude extract preparation protocol and optimizing reaction conditions, we were able to achieve active enhanced green fluorescent protein (EGFP) yields of greater than 50 µg/mL with batch reactions lasting up to 3 h...
January 23, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28094796/the-evolution-of-no-cost-resistance-at-sub-mic-concentrations-of-streptomycin-in-streptomyces-coelicolor
#5
Sanne Westhoff, Tim Marijn van Leeuwe, Omar Qachach, Zheren Zhang, Gilles Philippus van Wezel, Daniel Eric Rozen
At the high concentrations used in medicine, antibiotics exert strong selection on bacterial populations for the evolution of resistance. However, these lethal concentrations may not be representative of the concentrations bacteria face in soil, a recognition that has led to questions of the role of antibiotics in soil environments as well as the dynamics of resistance evolution during sublethal challenge. Here we examine the evolution of resistance to sub-minimal inhibitory concentrations (sub-MIC) of streptomycin in the filamentous soil bacterium Streptomyces coelicolor...
January 17, 2017: ISME Journal
https://www.readbyqxmd.com/read/28088540/multiplexed-site-specific-genome-engineering-for-overproducing-bioactive-secondary-metabolites-in-actinomycetes
#6
Lei Li, Guosong Zheng, Jun Chen, Mei Ge, Weihong Jiang, Yinhua Lu
Actinomycetes produce a large variety of pharmaceutically active compounds, yet production titers often require to be improved for discovery, development and large-scale manufacturing. Here, we describe a new technique, multiplexed site-specific genome engineering (MSGE) via the 'one integrase-multiple attB sites' concept, for the stable integration of secondary metabolite biosynthetic gene clusters (BGCs). Using MSGE, we achieved five-copy chromosomal integration of the pristinamycin II (PII) BGC in Streptomyces pristinaespiralis, resulting in the highest reported PII titers in flask and batch fermentations (2...
January 11, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28062460/large-scale-transposition-mutagenesis-of-streptomyces-coelicolor-identifies-hundreds-of-genes-influencing-antibiotic-biosynthesis
#7
Zhong Xu, Yemin Wang, Keith F Chater, Hong-Yu Ou, H Howard Xu, Zixin Deng, Meifeng Tao
: Gram-positive Streptomyces bacteria produce thousands of bioactive secondary metabolites including antibiotics. To systematically investigate genes affecting secondary metabolism, we developed a hyperactive transposase-based Tn5 transposition system and employed it to mutagenize the model species Streptomyces coelicolor, leading to the identification of 51,443 transposition insertions. These insertions were distributed randomly along the chromosome except for some preferred regions associated with relatively low GC content in the chromosomal core...
January 6, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28042035/structural-and-functional-characterization-of-the-alanine-racemase-from-streptomyces-coelicolor-a3-2
#8
Raffaella Tassoni, Lizah T van der Aart, Marcellus Ubbink, Gilles P van Wezel, Navraj S Pannu
The conversion of l-alanine (L-Ala) into d-alanine (D-Ala) in bacteria is performed by pyridoxal phosphate-dependent enzymes called alanine racemases. D-Ala is an essential component of the bacterial peptidoglycan and hence required for survival. The Gram-positive bacterium Streptomyces coelicolor has at least one alanine racemase encoded by alr. Here, we describe an alr deletion mutant of S. coelicolor which depends on D-Ala for growth and shows increased sensitivity to the antibiotic d-cycloserine (DCS)...
January 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28035598/rraas1-inhibits-the-ribonucleolytic-activity-of-rnase-es-by-interacting-with-its-catalytic-domain-in-streptomyces-coelicolor
#9
Sojin Seo, Daeyoung Kim, Wooseok Song, Jihune Heo, Minju Joo, Yeri Lim, Ji-Hyun Yeom, Kangseok Lee
RraA is a protein inhibitor of RNase E, which degrades and processes numerous RNAs in Escherichia coli. Streptomyces coelicolor also contains homologs of RNase E and RraA, RNase ES and RraAS1/RraAS2, respectively. Here, we report that, unlike other RraA homologs, RraAS1 directly interacts with the catalytic domain of RNase ES to exert its inhibitory effect. We further show that rraAS1 gene deletion in S. coelicolor results in a higher growth rate and increased production of actinorhodin and undecylprodigiosin, compared with the wild-type strain, suggesting that RraAS1-mediated regulation of RNase ES activity contributes to modulating the cellular physiology of S...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28003200/genome-integration-and-excision-by-a-new-streptomyces-bacteriophage-%C3%AF-joe
#10
Paul C M Fogg, Joshua A Haley, W Marshall Stark, Margaret C M Smith
Bacteriophages are the source of many valuable tools for molecular biology and genetic manipulation. In Streptomyces, most DNA cloning vectors are based on serine integrase site-specific DNA recombination systems derived from phage. Because of their efficiency and simplicity, serine integrases are also used for diverse synthetic biology applications. Here, we present the genome of a new Streptomyces phage, ϕJoe, and investigate the conditions for integration and excision of the ϕJoe genome. ϕJoe belongs to the largest Streptomyces phage cluster (R4-like) and encodes a serine integrase...
March 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27981234/coordinate-regulation-of-antimycin-and-candicidin-biosynthesis
#11
Thomas C McLean, Paul A Hoskisson, Ryan F Seipke
Streptomyces species produce an incredible array of high-value specialty chemicals and medicinal therapeutics. A single species typically harbors ~30 biosynthetic pathways, but only a few them are expressed in the laboratory; thus, poor understanding of how natural-product biosynthesis is regulated is a major bottleneck in drug discovery. Antimycins are a large family of anticancer compounds widely produced by Streptomyces species, and their regulation is atypical compared to that of most other natural products...
November 2016: MSphere
https://www.readbyqxmd.com/read/27966890/a-novel-approach-for-gene-expression-optimization-through-native-promoter-and-5-utr-combinations-based-on-rna-seq-ribo-seq-and-tss-seq-of-streptomyces-coelicolor
#12
Jeong Sang Yi, Min Woo Kim, Minsuk Kim, Yujin Jeong, Eun-Jung Kim, Byung-Kwan Cho, Byung-Gee Kim
Streptomycetes are Gram-positive mycelial bacteria, which synthesize a wide range of natural products including over two-thirds of the currently available antibiotics. However, metabolic engineering in Streptomyces species to overproduce a vast of natural products are hampered by a limited number of genetic tools. Here, two promoters and four 5' UTR sequences showing constant strengths were selected based upon multiomics data sets from Streptomyces coelicolor M145, including RNA-seq, Ribo-seq, and TSS-seq, for controllable transcription and translation...
December 22, 2016: ACS Synthetic Biology
https://www.readbyqxmd.com/read/27956713/characterization-of-a-biflaviolin-synthase-cyp158a3-from-streptomyces-avermitilis-and-its-role-in-the-biosynthesis-of-secondary-metabolites
#13
Young-Ran Lim, Songhee Han, Joo-Hwan Kim, Hyoung-Goo Park, Ga-Young Lee, Thien-Kim Le, Chul-Ho Yun, Donghak Kim
Streptomyces avermitilis produces clinically useful drugs such as avermectins and oligomycins. Its genome contains approximately 33 cytochrome P450 genes and they seem to play important roles in the biosynthesis of many secondary metabolites. The SAV_7130 gene from S. avermitilis encodes CYP158A3. The amino acid sequence of this enzyme has high similarity with that of CYP158A2, a biflaviolin synthase from S. coelicolor A3(2). Recombinant S. avermitilis CYP158A3 was heterologously expressed and purified. It exhibited the typical P450 Soret peak at 447 nm in the reduced CO-bound form...
March 1, 2017: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27936128/crystal-structure-of-the-streptomyces-coelicolor-sortase-e1-transpeptidase-provides-insight-into-the-binding-mode-of-the-novel-class-e-sorting-signal
#14
Michele D Kattke, Albert H Chan, Andrew Duong, Danielle L Sexton, Michael R Sawaya, Duilio Cascio, Marie A Elliot, Robert T Clubb
Many species of Gram-positive bacteria use sortase transpeptidases to covalently affix proteins to their cell wall or to assemble pili. Sortase-displayed proteins perform critical and diverse functions for cell survival, including cell adhesion, nutrient acquisition, and morphological development, among others. Based on their amino acid sequences, there are at least six types of sortases (class A to F enzymes); however, class E enzymes have not been extensively studied. Class E sortases are used by soil and freshwater-dwelling Actinobacteria to display proteins that contain a non-canonical LAXTG sorting signal, which differs from 90% of known sorting signals by substitution of alanine for proline...
2016: PloS One
https://www.readbyqxmd.com/read/27919888/identification-of-a-novel-lincomycin-resistance-mutation-associated-with-activation-of-antibiotic-production-in-streptomyces-coelicolor-a3-2
#15
Guojun Wang, Masumi Izawa, Xiaoge Yang, Dongbo Xu, Yukinori Tanaka, Kozo Ochi
Comparative genome sequencing analysis of a lincomycin-resistant strain of Streptomyces coelicolor A3(2) and the wild-type strain identified a novel mutation conferring a high level of lincomycin resistance. Surprisingly, the new mutation was an in-frame DNA deletion in the genes SCO4597 and SCO4598, resulting in formation of the hybrid gene linR. SCO4597 and SCO4598 encode two histidine kinases, which together with SCO4596, encoding a response regulator, constitute a unique two-component system. Sequence analysis indicated that these three genes and their arrangement patterns are ubiquitous among all Streptomyces genomes sequenced to date, suggesting these genes play important regulatory roles...
February 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27902442/the-whcd-gene-of-corynebacterium-glutamicum-plays-roles-in-cell-division-and-envelope-formation
#16
Dong-Seok Lee, Younhee Kim, Heung-Shick Lee
In this study, we analyzed the whcD gene from Corynebacterium glutamicum, which encodes a homolog of whiB, a Streptomyces coelicolor gene required for the sporulation of aerial hyphae. Deletion of the gene (ΔwhcD) severely affected cell growth in C. glutamicum. The ΔwhcD strain exhibited a large, filamentous, branched, and bud-shaped morphology with multiple septa. The transcription levels of the cell division genes involved in Z-ring assembly and septal peptidoglycan synthesis, including ftsZ, sepF, ftsQ, and ftsI, were markedly decreased in the ΔwhcD strain...
November 24, 2016: Microbiology
https://www.readbyqxmd.com/read/27897367/bifunctionality-of-activ-as-a-cyclase-thioesterase-revealed-by-in-vitro-reconstitution-of-actinorhodin-biosynthesis-in-streptomyces-coelicolor-a3-2
#17
Takaaki Taguchi, Takayoshi Awakawa, Yukitaka Nishihara, Michiho Kawamura, Yasuo Ohnishi, Koji Ichinose
Type II polyketide synthases iteratively generate a nascent polyketide thioester of the acyl carrier protein (ACP); this is structurally modified to produce an ACP-free intermediate towards the final metabolite. However, the timing of ACP off-loading is not well defined because of the lack of an apparent thioesterase (TE) among relevant biosynthetic enzymes. Here, ActIV, which had been assigned as a second ring cyclase (CYC) in actinorhodin (ACT) biosynthesis, was shown to possess TE activity in vitro with a model substrate, anthraquinone-2-carboxylic acid-N-acetylcysteamine...
November 29, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27875313/direct-involvement-of-the-master-nitrogen-metabolism-regulator-glnr-in-antibiotic-biosynthesis-in-streptomyces
#18
Juan-Mei He, Hong Zhu, Guo-Song Zheng, Pan-Pan Liu, Jin Wang, Guo-Ping Zhao, Guo-Qiang Zhu, Wei-Hong Jiang, Yin-Hua Lu
GlnR, an OmpR-like orphan two-component system response regulator, is a master regulator of nitrogen metabolism in the genus Streptomyces In this work, evidence that GlnR is also directly involved in the regulation of antibiotic biosynthesis is provided. In the model strain Streptomyces coelicolor M145, an in-frame deletion of glnR resulted in markedly increased actinorhodin (ACT) production but reduced undecylprodigiosin (RED) biosynthesis when exposed to R2YE culture medium. Transcriptional analysis coupled with DNA binding studies revealed that GlnR represses ACT but activates RED production directly via the pathway-specific activator genes actII-ORF4 and redZ, respectively...
December 16, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27822533/osdr-of-streptomyces-coelicolor-and-the-dormancy-regulator-devr-of-mycobacterium-tuberculosis-control-overlapping-regulons
#19
Mia Urem, Teunke van Rossum, Giselda Bucca, Geri F Moolenaar, Emma Laing, Magda A Świątek-Połatyńska, Joost Willemse, Elodie Tenconi, Sébastien Rigali, Nora Goosen, Colin P Smith, Gilles P van Wezel
Two-component regulatory systems allow bacteria to respond adequately to changes in their environment. In response to a given stimulus, a sensory kinase activates its cognate response regulator via reversible phosphorylation. The response regulator DevR activates a state of dormancy under hypoxia in Mycobacterium tuberculosis, allowing this pathogen to escape the host defense system. Here, we show that OsdR (SCO0204) of the soil bacterium Streptomyces coelicolor is a functional orthologue of DevR. OsdR, when activated by the sensory kinase OsdK (SCO0203), binds upstream of the DevR-controlled dormancy genes devR, hspX, and Rv3134c of M...
May 2016: MSystems
https://www.readbyqxmd.com/read/27797866/a-role-of-nitrite-reductase-nirbd-for-no-homeostatic-regulation-in-streptomyces-coelicolor-a3-2
#20
Yuriya Yukioka, Tsukiko Tanahashi, Keisuke Shida, Haruka Oguchi, Shota Ogawa, Chiaki Saito, Shunsuke Yajima, Shinsaku Ito, Kanju Ohsawa, Hirofumi Shoun, Yasuyuki Sasaki
Although nitric oxide (NO) is an important signaling molecule in bacteria and higher organisms, excessive intracellular NO is highly reactive and dangerous. Therefore, living cells need strict regulation systems for cellular NO homeostasis. Recently, we discovered that Streptomyces coelicolor A3(2) retains the nitrogen oxide cycle (NO3(-)→NO2(-)→NO→NO3(-)) and nitrite removal system. The nitrogen oxide cycle regulates cellular NO levels, thereby controlling secondary metabolism initiation (red-pigmented antibiotic, RED production) and morphological differentiation...
January 2017: FEMS Microbiology Letters
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