Viola Vaňková Hausnerová, Mahmoud Shoman, Dilip Kumar, Marek Schwarz, Martin Modrák, Jitka Jirát Matějčková, Eliška Mikesková, Silvia Neva, Anna Herrmannová, Michaela Šiková, Petr Halada, Iva Novotná, Petr Pajer, Leoš Shivaya Valášek, Martin Převorovský, Libor Krásný, Jarmila Hnilicová
Bacteria have evolved structured RNAs that can associate with RNA polymerase (RNAP). Two of them have been known so far-6S RNA and Ms1 RNA but it is unclear if any other types of RNAs binding to RNAP exist in bacteria. To identify all RNAs interacting with RNAP and the primary σ factors, we have established and performed native RIP-seq in Bacillus subtilis, Corynebacterium glutamicum, Streptomyces coelicolor, Mycobacterium smegmatis and the pathogenic Mycobacterium tuberculosis. Besides known 6S RNAs in B...
February 13, 2024: Nucleic Acids Research