keyword
MENU ▼
Read by QxMD icon Read
search

Streptomyces coelicolor

keyword
https://www.readbyqxmd.com/read/28906045/actinorhodin-is-a-redox-active-antibiotic-with-a-complex-mode-of-action-against-gram-positive-cells
#1
Stefanie Mak, Justin R Nodwell
Actinorhodin is a blue-pigmented, redox-active secondary metabolite that is produced by the bacterium Streptomyces coelicolor. Although actinorhodin has been used as a model compound for studying secondary metabolism, its biological activity is not well understood. Indeed, redox-active antibiotics in general have not been widely investigated at the mechanistic level. In this work, we have conducted a comprehensive chemical genetic investigation of actinorhodin's antibacterial effect on target organisms. We find that actinorhodin is a potent, bacteriostatic, pH-responsive antibiotic...
September 14, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28900375/expression-and-purification-of-untagged-glnk-proteins-from-actinobacteria
#2
Edileusa C M Gerhardt, Vivian R Moure, Andrey W Souza, Fabio O Pedrosa, Emanuel M Souza, Lautaro Diacovich, Hugo Gramajo, Luciano F Huergo
The PII protein family constitutes one of the most conserved and well distributed family of signal transduction proteins in nature. These proteins play key roles in nitrogen and carbon metabolism. PII function has been well documented in Gram-negative bacteria. However, there are very few reports describing the in vitro properties and function of PII derived from Gram-positive bacteria. Here we present the heterologous expression and efficient purification protocols for untagged PII from three Actinobacteria of medical and biotechnological interest namely: Mycobacterium tuberculosis, Rhodococcus jostii and Streptomyces coelicolor...
2017: EXCLI journal
https://www.readbyqxmd.com/read/28884676/the-mtrab-two-component-system-controls-antibiotic-production-in-streptomyces-coelicolor-a3-2
#3
Nicolle F Som, Daniel Heine, Neil Holmes, Felicity Knowles, Govind Chandra, Ryan F Seipke, Paul A Hoskisson, Barrie Wilkinson, Matthew I Hutchings
MtrAB is a highly conserved two-component system implicated in the regulation of cell division in the Actinobacteria. It coordinates DNA replication with cell division in the unicellular Mycobacterium tuberculosis and links antibiotic production to sporulation in the filamentous Streptomyces venezuelae. Chloramphenicol biosynthesis is directly regulated by MtrA in S. venezuelae and deletion of mtrB constitutively activates MtrA and results in constitutive over-production of chloramphenicol. Here we report that in Streptomyces coelicolor, MtrA binds to sites upstream of developmental genes and the genes encoding ActII-1, ActII-4 and RedZ, which are cluster-situated regulators of the antibiotics actinorhodin (Act) and undecylprodigiosin (Red)...
September 8, 2017: Microbiology
https://www.readbyqxmd.com/read/28822070/chemical-shift-assignments-for-the-apo-form-of-the-catalytic-domain-the-linker-region-and-the-carbohydrate-binding-domain-of-the-cellulose-active-lytic-polysaccharide-monooxygenase-sclpmo10c
#4
Gaston Courtade, Zarah Forsberg, Gustav Vaaje-Kolstad, Vincent G H Eijsink, Finn L Aachmann
The apo-form of the 21.4 kDa catalytic domain and the 10.7 kDa carbohydrate binding domain of the AA10 family lytic polysaccharide monooxygenase ScLPMO10C from Streptomyces coelicolor have been isotopically labeled and recombinantly expressed in Escherichia coli. In this paper, we report the (1)H, (13)C, and (15)N chemical shift assignments of each individual domain as well as an ensemble of the assignment for the full-length protein, including its approximately 30-amino acid long linker.
August 18, 2017: Biomolecular NMR Assignments
https://www.readbyqxmd.com/read/28812299/conversion-of-the-high-yield-salinomycin-producer-streptomyces-albus-bk3-25-into-a-surrogate-host-for-polyketide-production
#5
Xiaojie Zhang, Chenyang Lu, Linquan Bai
An ideal surrogate host for heterologous production of various natural products is expected to have efficient nutrient utilization, fast growth, abundant precursors and energy supply, and a pronounced gene expression. Streptomyces albus BK3-25 is a high-yield industrial strain producing type-I polyketide salinomycin, with a unique ability of bean oil utilization. Its potential of being a surrogate host for heterologous production of PKS was engineered and evaluated herein. Firstly, introduction of a three-gene cassette for the biosynthesis of ethylmalonyl-CoA resulted in accumulation of ethylmalonyl-CoA precursor and salinomycin, and subsequent deletion of the salinomycin biosynthetic gene cluster resulted in a host with rich supplies of common polyketide precursors, including malonyl-CoA, methylmalonyl-CoA, and ethylmalonyl-CoA...
August 14, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28783117/the-role-of-functional-amyloids-in-multicellular-growth-and-development-of-gram-positive-bacteria
#6
REVIEW
Anna Dragoš, Ákos T Kovács, Dennis Claessen
Amyloid fibrils play pivotal roles in all domains of life. In bacteria, these fibrillar structures are often part of an extracellular matrix that surrounds the producing organism and thereby provides protection to harsh environmental conditions. Here, we discuss the role of amyloid fibrils in the two distant Gram-positive bacteria, Streptomyces coelicolor and Bacillus subtilis. We describe how amyloid fibrils contribute to a multitude of developmental processes in each of these systems, including multicellular growth and community development...
August 7, 2017: Biomolecules
https://www.readbyqxmd.com/read/28782575/toxicological-evaluation-of-neoagarooligosaccharides-prepared-by-enzymatic-hydrolysis-of-agar
#7
Sun Joo Hong, Je-Hyeon Lee, Eun Joo Kim, Hea Jung Yang, Jae-Seon Park, Soon-Kwang Hong
Agar, a heterogeneous polymer of galactose, is the main component of the cell wall of marine red algae. It is well established as a safe, non-digestible carbohydrate in Oriental countries. Although neoagarooligosaccharides (NAOs) prepared by the hydrolysis of agar by β-agarase have been reported to exert various biological activities, the safety of these compounds has not been reported to date. For safety evaluation, NAOs containing mainly neoagarotetraose and neoagarohexaose were prepared from agar by enzymatic hydrolysis using β-agarase DagA from Streptomyces coelicolor...
August 3, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28761057/development-of-series-of-affinity-tags-in-streptomyces
#8
Xu-Ming Mao, Ning Sun, Yang Zheng, Yong-Quan Li
Streptomyces are of great biological and industrial significance due to their complex morphological development and ability to produce numerous secondary metabolites. However, the intrinsic biochemical mechanisms underlying morphogenesis and secondary metabolism are rarely revealed, partially because of the limited availability of the biochemical tools in Streptomyces. Here we provided series of integrative vectors with various affinity tags, including single tags 3×FLAG, 3×HA, 3×Strep-tag II, 18×His, 13×Myc, and dual tags, all of which were driven from a strong constitutive promoter ermEp*...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28752886/correction-zwitterionic-pyrrolidene-phosphonates-inhibitors-of-the-glycoside-hydrolase-like-phosphorylase-streptomyces-coelicolor-glgei-v279s
#9
Sri Kumar Veleti, Cecile Petit, Jared J Lindenberger, Donald R Ronning, Steven J Sucheck
Correction for 'Zwitterionic pyrrolidene-phosphonates: inhibitors of the glycoside hydrolase-like phosphorylase Streptomyces coelicolor GlgEI-V279S' by Sri Kumar Veleti et al., Org. Biomol. Chem., 2017, 15, 3884-3891.
August 9, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28730324/the-role-of-ndgr-in-glycerol-metabolism-in-streptomyces-coelicolor
#10
Bo-Rahm Lee, Shashi Kant Bhatia, Hun-Suk Song, Junyoung Kim, Wooseong Kim, Hyung-Yeon Park, Jeong-Jun Yoon, Sung-Hee Park, Daehee Hwang, Byung-Gee Kim, Yung-Hun Yang
Streptomyces, which produces many pharmaceutical antibiotics and anticancer agents, is a genus of soil-dwelling bacteria with numerous regulators that control both primary and secondary metabolism. NdgR is highly conserved in Streptomyces spp. and is known to be involved in antibiotic production, tolerance against shock and physical stress, nitrogen metabolism, leucine metabolism, and N-acetylglucosamine metabolism. As another function of NdgR, we report the involvement of NdgR in glycerol metabolism in S. coelicolor...
July 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28729266/draft-genome-sequence-of-streptomyces-sp-m1013-a-close-relative-of-streptomyces-ambofaciens-and-streptomyces-coelicolor
#11
Drago Haas, Claude Gerbaud, Nevzat Sahin, Jean-Luc Pernodet, Sylvie Lautru
We report the draft genome sequence of Streptomyces sp. M1013, a strain isolated from the Medicago arborea rhizosphere in Izmir, Turkey. An average nucleotide identity (ANI) analysis reveals that this strain belongs to the same species as Streptomyces canus ATCC12647 and is closely related to Streptomyces ambofaciens and Streptomyces coelicolor.
July 20, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28692281/structural-and-biophysical-characterization-of-the-mycobacterium-tuberculosis-protein-rv0577-a-protein-associated-with-neutral-red-staining-of-virulent-tuberculosis-strains-and-homologue-of-the-streptomyces-coelicolor-protein-kbpa
#12
COMPARATIVE STUDY
Garry W Buchko, Nathaniel Echols, E Megan Flynn, Ho-Leung Ng, Samuel Stephenson, Heung-Bok Kim, Peter J Myler, Thomas C Terwilliger, Tom Alber, Chang-Yub Kim
Mycobacterium tuberculosis protein Rv0577 is a prominent antigen in tuberculosis patients, the component responsible for neutral red staining of virulent strains of M. tuberculosis, a putative component in a methylglyoxal detoxification pathway, and an agonist of toll-like receptor 2. It also has an amino acid sequence that is 36% identical to that of Streptomyces coelicolor AfsK-binding protein A (KbpA), a component in the complex secondary metabolite pathways in the Streptomyces genus. To gain insight into the biological function of Rv0577 and the family of KpbA kinase regulators, the crystal structure for Rv0577 was determined to a resolution of 1...
August 1, 2017: Biochemistry
https://www.readbyqxmd.com/read/28640671/regulation-of-a-muralytic-enzyme-encoding-gene-by-two-non-coding-rnas
#13
Renée J St-Onge, Marie A Elliot
Non-coding regulatory RNAs fine-tune gene expression post-transcriptionally. In the streptomycetes, rpfA - encoding a muralytic enzyme required for establishing and exiting dormancy - is flanked by non-coding regulatory RNA elements both upstream (riboswitch) and downstream [antisense small RNA (sRNA)]. In Streptomyces coelicolor, the upstream riboswitch decreases rpfA transcript abundance in response to the second messenger cyclic di-AMP, itself involved in cell wall metabolism and dormancy. There is, however, no obvious expression platform associated with this riboswitch and consequently, its mechanism of action is entirely unknown...
June 22, 2017: RNA Biology
https://www.readbyqxmd.com/read/28631385/identification-of-glucose-kinase-dependent-and-independent-pathways-for-carbon-control-of-primary-metabolism-development-and-antibiotic-production-in-streptomyces-coelicolor-by-quantitative-proteomics
#14
Jacob Gubbens, Marleen M Janus, Bogdan I Florea, Herman S Overkleeft, Gilles P van Wezel
No abstract text is available yet for this article.
July 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28601893/characterization-of-an-lrp-asnc-family-regulator-sco3361-controlling-actinorhodin-production-and-morphological-development-in-streptomyces-coelicolor
#15
Jing Liu, Jie Li, Hong Dong, Yunfu Chen, Yansheng Wang, Hang Wu, Changrun Li, David T Weaver, Lixin Zhang, Buchang Zhang
Lrp/AsnC family regulators have been found in many bacteria as crucial regulators controlling diverse cellular processes. By genomic alignment, we found that SCO3361, an Lrp/AsnC family protein from Streptomyces coelicolor, shared the highest similarity to the SACE_Lrp from Saccharopolyspora erythraea. Deletion of SCO3361 led to dramatic reduction in actinorhodin (Act) production and delay in aerial mycelium formation and sporulation on solid media. Dissection of the mechanism underlying the function of SCO3361 in Act production revealed that it altered the transcription of the cluster-situated regulator gene actII-ORF4 by directly binding to its promoter...
June 11, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28599184/plasma-modified-pla-electrospun-membranes-for-actinorhodin-production-intensification-in-streptomyces-coelicolor-immobilized-cell-cultivations
#16
Roberto Scaffaro, Francesco Lopresti, Alberto Sutera, Luigi Botta, Rosa Maria Fontana, Giuseppe Gallo
Most of industrially relevant bioproducts are produced by submerged cultivations of actinomycetes. The immobilization of these Gram-positive filamentous bacteria on suitable porous supports may prevent mycelial cell-cell aggregation and pellet formation which usually negatively affect actinomycete submerged cultivations, thus, resulting in an improved biosynthetic capability. In this work, electrospun polylactic acid (PLA) membranes, subjected or not to O2-plasma treatment (PLA-plasma), were used as support for immobilized-cell submerged cultivations of Streptomyces coelicolor M145...
September 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28598435/zinc-dependent-regulation-of-zinc-import-and-export-genes-by-zur
#17
Seung-Hwan Choi, Kang-Lok Lee, Jung-Ho Shin, Yoo-Bok Cho, Sun-Shin Cha, Jung-Hye Roe
In most bacteria, zinc depletion is sensed by Zur, whereas the surplus is sensed by different regulators to achieve zinc homeostasis. Here we present evidence that zinc-bound Zur not only represses genes for zinc acquisition but also induces the zitB gene encoding a zinc exporter in Streptomyces coelicolor, a model actinobacteria. Zinc-dependent gene regulation by Zur occurs in two phases. At sub-femtomolar zinc concentrations (phase I), dimeric Zur binds to the Zur-box motif immediately upstream of the zitB promoter, resulting in low zitB expression...
June 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/28569150/heterologous-expression-of-pikromycin-biosynthetic-gene-cluster-using-streptomyces-artificial-chromosome-system
#18
Hye-Rim Pyeon, Hee-Ju Nah, Seung-Hoon Kang, Si-Sun Choi, Eung-Soo Kim
BACKGROUND: Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully applied for precise cloning and tandem integration of a large polyketide tautomycetin (TMC) biosynthetic gene cluster (Nah et al. in Microb Cell Fact 14(1):1, 2015), implying that this strategy could be employed to develop a custom overexpression scheme of natural product pathway clusters present in actinomycetes...
May 31, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28560880/quantitative-proteomics-analysis-confirmed-oxidative-metabolism-predominates-in-streptomyces-coelicolor-versus-glycolytic-metabolism-in-streptomyces-lividans
#19
Aaron Millan-Oropeza, Céline Henry, Mélisande Blein-Nicolas, Anne Aubert-Frambourg, Fathi Moussa, Jean Bleton, Marie-Jöelle Virolle
Recent physiological studies indicated that S. lividans metabolism was mainly glycolytic, whereas S. coelicolor metabolism was mainly oxidative. To determine whether such metabolic characteristics were correlated with consistent proteomics features, a comparative label-free, shotgun proteomics analysis of these strains was carried out. Among 2024 proteins identified, 360 showed significant differences in abundance between the strains. This study revealed that S. coelicolor catabolized glucose less actively than S...
June 15, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28553520/watasemycin-biosynthesis-in-streptomyces-venezuelae-thiazoline-c-methylation-by-a-type-b-radical-sam-methylase-homologue
#20
Yuki Inahashi, Shanshan Zhou, Maureen J Bibb, Lijiang Song, Mahmoud M Al-Bassam, Mervyn J Bibb, Gregory L Challis
2-Hydroxyphenylthiazolines are a family of iron-chelating nonribosomal peptide natural products that function as virulence-conferring siderophores in various Gram-negative bacteria. They have also been reported as metabolites of Gram-positive Streptomyces species. Transcriptional analyses of Streptomyces venezuelae ATCC 10712 revealed that its genome contains a putative 2-hydroxyphenylthiazoline biosynthetic gene cluster. Heterologous expression of the gene cluster in Streptomyces coelicolor M1152 showed that the mono- and dimethylated derivatives, thiazostatin and watasemycin, respectively, of the 2-hydroxyphenylthiazoline enantiopyochelin are two of its metabolic products...
April 1, 2017: Chemical Science
keyword
keyword
17073
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"