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Streptomyces coelicolor

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https://www.readbyqxmd.com/read/28343010/structural-and-dynamics-studies-of-the-tetr-family-protein-cprb-from-streptomyces-coelicolor-in-complex-with-its-biological-operator-sequence
#1
Hussain Bhukya, Asis K Jana, Neelanjana Sengupta, Ruchi Anand
In Streptomycetes, tetracycline repressor family of transcription regulators (TetR-FTRs) controls various biological processes including antibiotic biosynthesis, cellular morphology and innate resistance. Here, we focus on understanding the structural basis of transcription regulation by CprB, a member of TetR-FTRs from S. coelicolor. CprB is implicated as a receptor of γ-butyrolactones, a class of quorum sensing molecules, responsible for initiating secondary metabolic pathways. In order to understand the molecular mechanism of DNA recognition, the X-ray structure of CprB in complex with its biological relevant operator sequence was solved to a resolution of 3...
March 22, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28338903/disulfide-bridges-as-essential-elements-for-the-thermostability-of-lytic-polysaccharide-monooxygenase-lpmo10c-from-streptomyces-coelicolor
#2
Magali Tanghe, Barbara Danneels, Matthias Last, Koen Beerens, Ingeborg Stals, Tom Desmet
Lytic polysaccharide monooxygenases (LPMOs) are crucial components of cellulase mixtures but their stability has not yet been studied in detail, let alone been engineered for industrial applications. In this work, we have evaluated the importance of disulfide bridges for the thermodynamic stability of Streptomyces coelicolor LPMO10C. Interestingly, this enzyme was found to retain 34% of its activity after 2-h incubation at 80°C while its apparent melting temperature (Tm) is only 51°C. When its three disulfide bridges were broken, however, irreversible unfolding occurred and no residual activity could be detected after a similar heat treatment...
March 9, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/28333098/anti-obesity-and-anti-diabetic-effect-of-neoagarooligosaccharides-on-high-fat-diet-induced-obesity-in-mice
#3
Sun Joo Hong, Je-Hyeon Lee, Eun Joo Kim, Hea Jung Yang, Jae-Seon Park, Soon-Kwang Hong
Neoagarooligosaccharides (NAOs), mainly comprising neoagarotetraose and neoagarohexaose, were prepared by hydrolyzing agar with β-agarase DagA from Streptomyces coelicolor, and the anti-obesity and anti-diabetic effects of NAOs on high-fat diet (HFD)-induced obesity in mice were investigated after NAOs-supplementation for 64 days. Compared to the HFD group, the HFD-0.5 group that was fed with HFD + NAOs (0.5%, w/w) showed remarkable reduction of 36% for body weight gain and 37% for food efficiency ratios without abnormal clinical signs...
March 23, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28331973/green-fluorescent-protein-as-a-reporter-for-the-spatial-and-temporal-expression-of-actiii-in-streptomyces-coelicolor
#4
Fernando Santos-Beneit, Jeff Errington
Polyketides constitute a large group of structurally diverse natural products with useful biological activities. Insights into their biosynthetic mechanisms are crucial for developing new structures. One of the most studied model polyketide is the blue-pigmented antibiotic actinorhodin, produced by Streptomyces coelicolor. This aromatic polyketide is synthesized by minimal type II polyketide synthases and tailoring enzymes. The ActIII actinorhodin ketoreductase is a key tailoring enzyme in actinorhodin biosynthesis...
March 22, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28330295/biomass-derived-molecules-modulate-the-behavior-of-streptomyces-coelicolor-for-antibiotic-production
#5
Shashi Kant Bhatia, Bo-Rahm Lee, Ganesan Sathiyanarayanan, Hun Seok Song, Junyoung Kim, Jong-Min Jeon, Jeong-Jun Yoon, Jungoh Ahn, Kyungmoon Park, Yung-Hun Yang
Various chemicals, i.e., furfural, vanillin, 4-hydroxybenzaldehyde and acetate produced during the pretreatment of biomass affect microbial fermentation. In this study, effect of vanillin, 4-hydroxybenzaldehyde and acetate on antibiotic production in Streptomyces coelicolor is investigated. IC 50 value of vanillin, 4-hydroxybenzaldehyde and acetate was recorded as 5, 11.3 and 115 mM, respectively. Vanillin was found as a very effective molecule, and it completely abolished antibiotic (undecylprodigiosin and actinorhodin) production at 1 mM concentration, while 4-hydroxybenzaldehyde and acetate have little effect...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28298624/strong-antibiotic-production-is-correlated-with-highly-active-oxidative-metabolism-in-streptomyces-coelicolor-m145
#6
Catherine Esnault, Thierry Dulermo, Aleksey Smirnov, Ahmed Askora, Michelle David, Ariane Deniset-Besseau, Ian-Barry Holland, Marie-Joelle Virolle
The Streptomyces genus is well known for its ability to produce bio-active secondary metabolites of great medical interest. However, the metabolic features accompanying these bio-productions remain to be defined. In this study, the comparison of related model strains producing differing levels of actinorhoddin (ACT), showed that S. lividans, a weak producer, had high TriAcylGlycerol (TAG) content indicative of a glycolytic metabolism. In contrast, the strong producer, S. coelicolor, was characterized by low TAG content, active consumption of its polyphosphate (PolyP) stores and extremely high ATP/ADP ratios...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28293039/the-master-regulator-phop-coordinates-phosphate-and-nitrogen-metabolism-respiration-cell-differentiation-and-antibiotic-biosynthesis-comparison-in-streptomyces-coelicolor-and-streptomyces-avermitilis
#7
REVIEW
Juan F Martín, Antonio Rodríguez-García, Paloma Liras
Phosphate limitation is important for production of antibiotics and other secondary metabolites in Streptomyces. Phosphate control is mediated by the two-component system PhoR-PhoP. Following phosphate depletion, PhoP stimulates expression of genes involved in scavenging, transport and mobilization of phosphate, and represses the utilization of nitrogen sources. PhoP reduces expression of genes for aerobic respiration and activates nitrate respiration genes. PhoP activates genes for teichuronic acid formation and reduces expression of genes for phosphate-rich teichoic acid biosynthesis...
March 15, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28247198/development-and-application-of-a-t7-rna-polymerase-dependent-expression-system-for-antibiotic-production-improvement-in-streptomyces
#8
Junhong Wei, Jinjin Tian, Guoqing Pan, Jie Xie, Jialing Bao, Zeyang Zhou
OBJECTIVE: To develop a reliable and easy to use expression system for antibiotic production improvement of Streptomyces. RESULTS: A two-compound T7 RNA polymerase-dependent gene expression system was developed to fulfill this demand. In this system, the T7 RNA polymerase coding sequence was optimized based on the codon usage of Streptomyces coelicolor. To evaluate the functionality of this system, we constructed an activator gene overexpression strain for enhancement of actinorhodin production...
February 28, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28211492/the-propensity-of-the-bacterial-rodlin-protein-rdlb-to-form-amyloid-fibrils-determines-its-function-in-streptomyces-coelicolor
#9
Wen Yang, Joost Willemse, Elizabeth B Sawyer, Fei Lou, Weibin Gong, Hong Zhang, Sally L Gras, Dennis Claessen, Sarah Perrett
Streptomyces bacteria form reproductive aerial hyphae that are covered with a pattern of pairwise aligned fibrils called rodlets. The presence of the rodlet layer requires two homologous rodlin proteins, RdlA and RdlB, and the functional amyloid chaplin proteins, ChpA-H. In contrast to the redundancy shared among the eight chaplins, both RdlA and RdlB are indispensable for the establishment of this rodlet structure. By using a comprehensive biophysical approach combined with in vivo characterization we found that RdlB, but not RdlA, readily assembles into amyloid fibrils...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28210250/simultaneous-activation-of-iron-and-thiol-based-sensor-regulator-systems-by-redox-active-compounds
#10
Kang-Lok Lee, Ji-Sun Yoo, Gyeong-Seok Oh, Atul K Singh, Jung-Hye Roe
Bacteria in natural habitats are exposed to myriad redox-active compounds (RACs), which include producers of reactive oxygen species (ROS) and reactive electrophile species (RES) that alkylate or oxidize thiols. RACs can induce oxidative stress in cells and activate response pathways by modulating the activity of sensitive regulators. However, the effect of a certain compound on the cell has been investigated primarily with respect to a specific regulatory pathway. Since a single compound can exert multiple chemical effects in the cell, its effect can be better understood by time-course monitoring of multiple sensitive regulatory pathways that the compound induces...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28167521/ddar-pa1196-regulates-expression-of-dimethylarginine-dimethylaminohydrolase-for-the-metabolism-of-methylarginines-in-pseudomonas-aeruginosa-pao1
#11
Benjamin R Lundgren, Frank J Bailey, Gabriella Moley, Christopher T Nomura
Dimethylarginine dimethylaminohydrolases or DDAHs catalyze the hydrolysis of methylarginines to yield L-citrulline and methylamines as products. DDAHs and their central roles in methylarginine metabolism have been characterized for eukaryotic cells. While DDAHs are known to exist in some bacteria, including Streptomyces coelicolor and Pseudomonas aeruginosa, the physiological importance and genetic regulation of bacterial DDAHs remain poorly understood. To provide some insight into bacterial methylarginine metabolism, this study focused on identifying the key elements or factors regulating DDAH expression in P...
February 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28112394/establishing-a-high-yielding-streptomyces-based-cell-free-protein-synthesis-system
#12
Jian Li, He Wang, Yong-Chan Kwon, Michael C Jewett
Cell-free protein synthesis (CFPS) has emerged as a powerful platform for applied biotechnology and synthetic biology, with a range of applications in synthesizing proteins, evolving proteins, and prototyping genetic circuits. To expand the current CFPS repertoire, we report here the development and optimization of a Streptomyces-based CFPS system for the expression of GC-rich genes. By developing a streamlined crude extract preparation protocol and optimizing reaction conditions, we were able to achieve active enhanced green fluorescent protein (EGFP) yields of greater than 50 µg/mL with batch reactions lasting up to 3 h...
January 23, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28094796/the-evolution-of-no-cost-resistance-at-sub-mic-concentrations-of-streptomycin-in-streptomyces-coelicolor
#13
Sanne Westhoff, Tim Marijn van Leeuwe, Omar Qachach, Zheren Zhang, Gilles Philippus van Wezel, Daniel Eric Rozen
At the high concentrations used in medicine, antibiotics exert strong selection on bacterial populations for the evolution of resistance. However, these lethal concentrations may not be representative of the concentrations bacteria face in soil, a recognition that has led to questions of the role of antibiotics in soil environments as well as the dynamics of resistance evolution during sublethal challenge. Here we examine the evolution of resistance to sub-minimal inhibitory concentrations (sub-MIC) of streptomycin in the filamentous soil bacterium Streptomyces coelicolor...
January 17, 2017: ISME Journal
https://www.readbyqxmd.com/read/28088540/multiplexed-site-specific-genome-engineering-for-overproducing-bioactive-secondary-metabolites-in-actinomycetes
#14
Lei Li, Guosong Zheng, Jun Chen, Mei Ge, Weihong Jiang, Yinhua Lu
Actinomycetes produce a large variety of pharmaceutically active compounds, yet production titers often require to be improved for discovery, development and large-scale manufacturing. Here, we describe a new technique, multiplexed site-specific genome engineering (MSGE) via the 'one integrase-multiple attB sites' concept, for the stable integration of secondary metabolite biosynthetic gene clusters (BGCs). Using MSGE, we achieved five-copy chromosomal integration of the pristinamycin II (PII) BGC in Streptomyces pristinaespiralis, resulting in the highest reported PII titers in flask and batch fermentations (2...
January 11, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28062460/large-scale-transposition-mutagenesis-of-streptomyces-coelicolor-identifies-hundreds-of-genes-influencing-antibiotic-biosynthesis
#15
Zhong Xu, Yemin Wang, Keith F Chater, Hong-Yu Ou, H Howard Xu, Zixin Deng, Meifeng Tao
Gram-positive Streptomyces bacteria produce thousands of bioactive secondary metabolites, including antibiotics. To systematically investigate genes affecting secondary metabolism, we developed a hyperactive transposase-based Tn5 transposition system and employed it to mutagenize the model species Streptomyces coelicolor, leading to the identification of 51,443 transposition insertions. These insertions were distributed randomly along the chromosome except for some preferred regions associated with relatively low GC content in the chromosomal core...
March 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28042035/structural-and-functional-characterization-of-the-alanine-racemase-from-streptomyces-coelicolor-a3-2
#16
Raffaella Tassoni, Lizah T van der Aart, Marcellus Ubbink, Gilles P van Wezel, Navraj S Pannu
The conversion of l-alanine (L-Ala) into d-alanine (D-Ala) in bacteria is performed by pyridoxal phosphate-dependent enzymes called alanine racemases. D-Ala is an essential component of the bacterial peptidoglycan and hence required for survival. The Gram-positive bacterium Streptomyces coelicolor has at least one alanine racemase encoded by alr. Here, we describe an alr deletion mutant of S. coelicolor which depends on D-Ala for growth and shows increased sensitivity to the antibiotic d-cycloserine (DCS)...
January 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28035598/rraas1-inhibits-the-ribonucleolytic-activity-of-rnase-es-by-interacting-with-its-catalytic-domain-in-streptomyces-coelicolor
#17
Sojin Seo, Daeyoung Kim, Wooseok Song, Jihune Heo, Minju Joo, Yeri Lim, Ji-Hyun Yeom, Kangseok Lee
RraA is a protein inhibitor of RNase E, which degrades and processes numerous RNAs in Escherichia coli. Streptomyces coelicolor also contains homologs of RNase E and RraA, RNase ES and RraAS1/RraAS2, respectively. Here, we report that, unlike other RraA homologs, RraAS1 directly interacts with the catalytic domain of RNase ES to exert its inhibitory effect. We further show that rraAS1 gene deletion in S. coelicolor results in a higher growth rate and increased production of actinorhodin and undecylprodigiosin, compared with the wild-type strain, suggesting that RraAS1-mediated regulation of RNase ES activity contributes to modulating the cellular physiology of S...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28003200/genome-integration-and-excision-by-a-new-streptomyces-bacteriophage-%C3%AF-joe
#18
Paul C M Fogg, Joshua A Haley, W Marshall Stark, Margaret C M Smith
Bacteriophages are the source of many valuable tools for molecular biology and genetic manipulation. In Streptomyces, most DNA cloning vectors are based on serine integrase site-specific DNA recombination systems derived from phage. Because of their efficiency and simplicity, serine integrases are also used for diverse synthetic biology applications. Here, we present the genome of a new Streptomyces phage, ϕJoe, and investigate the conditions for integration and excision of the ϕJoe genome. ϕJoe belongs to the largest Streptomyces phage cluster (R4-like) and encodes a serine integrase...
March 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27981234/coordinate-regulation-of-antimycin-and-candicidin-biosynthesis
#19
Thomas C McLean, Paul A Hoskisson, Ryan F Seipke
Streptomyces species produce an incredible array of high-value specialty chemicals and medicinal therapeutics. A single species typically harbors ~30 biosynthetic pathways, but only a few them are expressed in the laboratory; thus, poor understanding of how natural-product biosynthesis is regulated is a major bottleneck in drug discovery. Antimycins are a large family of anticancer compounds widely produced by Streptomyces species, and their regulation is atypical compared to that of most other natural products...
November 2016: MSphere
https://www.readbyqxmd.com/read/27966890/a-novel-approach-for-gene-expression-optimization-through-native-promoter-and-5-utr-combinations-based-on-rna-seq-ribo-seq-and-tss-seq-of-streptomyces-coelicolor
#20
Jeong Sang Yi, Min Woo Kim, Minsuk Kim, Yujin Jeong, Eun-Jung Kim, Byung-Kwan Cho, Byung-Gee Kim
Streptomycetes are Gram-positive mycelial bacteria, which synthesize a wide range of natural products including over two-thirds of the currently available antibiotics. However, metabolic engineering in Streptomyces species to overproduce a vast of natural products are hampered by a limited number of genetic tools. Here, two promoters and four 5' UTR sequences showing constant strengths were selected based upon multiomics data sets from Streptomyces coelicolor M145, including RNA-seq, Ribo-seq, and TSS-seq, for controllable transcription and translation...
December 22, 2016: ACS Synthetic Biology
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