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Streptomyces coelicolor

Grace Yim, Wenliang Wang, Maulik N Thaker, Stephanie Tan, Gerard D Wright
Modification of natural product backbones is a proven strategy for the development of clinically useful antibiotics. Such modifications have traditionally been achieved through medicinal chemistry strategies or via in vitro enzymatic activities. In an orthogonal approach, engineering of biosynthetic pathways using synthetic biology techniques can generate chemical diversity. Here we report the use of a minimal teicoplanin class glycopeptide antibiotic (GPA) scaffold expressed in a production-optimized Streptomyces coelicolor strain to expand GPA chemical diversity...
September 9, 2016: ACS Infectious Diseases
Irene Saugar, Brian Molloy, Eloisa Sanz, María Blanca Sánchez, María Fernández-Lobato, Antonio Jiménez
Antibiotic A201A produced by Saccharothrix mutabilis subsp. capreolus NRRL3817 contains an aminonucleoside (N(6), N(6)-dimethyl-3'-amino-3'-deoxyadenosyl), a polyketide (α-methyl-p-coumaric acid) and a disaccharide moiety. The heterologous expression in Streptomyces lividans and Streptomyces coelicolor of a S. mutabilis genomic region of ~34 kb results in the production of A201A, which was identified by microbiological, biochemical and physicochemical approaches, and indicating that this region may contain the entire A201A biosynthetic gene cluster (ata)...
October 12, 2016: Journal of Antibiotics
Marina Borisova, Rosmarie Gaupp, Amanda Duckworth, Alexander Schneider, Désirée Dalügge, Maraike Mühleck, Denise Deubel, Sandra Unsleber, Wenqi Yu, Günther Muth, Markus Bischoff, Friedrich Götz, Christoph Mayer
: Peptidoglycan recycling is a metabolic process by which Gram-negative bacteria reutilize up to half of their cell wall within one generation during vegetative growth. Whether peptidoglycan recycling also occurs in Gram-positive bacteria has so far remained unclear. We show here that three Gram-positive model organisms, Staphylococcus aureus, Bacillus subtilis, and Streptomyces coelicolor, all recycle the sugar N-acetylmuramic acid (MurNAc) of their peptidoglycan during growth in rich medium...
October 11, 2016: MBio
Yue Zhang, Chun-Yan Lin, Xiao-Mei Li, Zheng-Kun Tang, Jianjun Qiao, Guang-Rong Zhao
The polyether ionophore antibiotic monensin is produced by Streptomyces cinnamonensis and is used as a coccidiostat for chickens and growth-promoting agent for cattle. Monensin biosynthetic gene cluster has been cloned and partially characterized. The GntR-family transcription factor DasR regulates antibiotic production and morphological development in Streptomyces coelicolor and Saccharopolyspora erythraea. In this study, we identified and characterized the two-level regulatory cascade of DasR to monensin production in S...
October 8, 2016: Journal of Industrial Microbiology & Biotechnology
Jihune Heo, Daeyoung Kim, Minju Joo, Boeun Lee, Sojin Seo, Jaejin Lee, Saemee Song, Ji-Hyun Yeom, Nam-Chul Ha, Kangseok Lee
RraA is a protein inhibitor of RNase E (Rne), which catalyzes the endoribonucleolytic cleavage of a large proportion of RNAs in Escherichia coli. The antibiotic-producing bacterium Streptomyces coelicolor also contains homologs of RNase E and RraA, designated as RNase ES (Rns), RraAS1, and RraAS2, respectively. Here, we report that RraAS2 requires both scaffold domains of RNase ES for high-affinity binding and inhibitory action on the ribonucleolytic activity. Analyses of the steady-state level of RNase E substrates indicated that coexpression of RraAS2 in E...
October 2016: Journal of Microbiology / the Microbiological Society of Korea
José M Camacho-Zaragoza, Georgina Hernández-Chávez, Fabian Moreno-Avitia, René Ramírez-Iñiguez, Alfredo Martínez, Francisco Bolívar, Guillermo Gosset
BACKGROUND: Resveratrol is a plant natural product with many health-protecting effects which makes it an attractive chemical both for academic studies and industrial purposes. However, the low quantities naturally produced by plants as well as the unsustainable procedures of extraction, purification and concentration have prompted many biotechnological approaches to produce this chemical in large quantities from renewable sources. None of these approaches have considered a microbial coculture strategy to produce this compound...
September 29, 2016: Microbial Cell Factories
Emilia Palazzotto, Giuseppe Gallo, Giovanni Renzone, Anna Giardina, Alberto Sutera, Joohee Silva, Celinè Vocat, Luigi Botta, Andrea Scaloni, Anna Maria Puglia
In the model actinomycete Streptomyces coelicolor A3(2), small open reading frames encoding proteins with unknown functions were identified in several amino acid biosynthetic gene operons, such as SCO2038 (trpX) in the tryptophan trpCXBA locus. In this study, the role of the corresponding protein in tryptophan biosynthesis was investigated by combining phenotypic and molecular analyses. The 2038KO mutant strain was characterized by delayed growth, smaller aerial hyphae and reduced production of spores and actinorhodin antibiotic, with respect to the WT strain...
2016: PloS One
Ya Xu, Cheng-Heng Liao, Li-Li Yao, Xu Ye, Bang-Ce Ye
Starch-degrading enzymes hydrolyze starch- and starch-derived oligosaccharides to yield glucose. We investigated the transcriptional regulation of genes encoding starch-degrading enzymes in the industrial actinobacteria Saccharopolyspora erythraea We observed that most genes encoding amylolytic enzymes (one α-amylase, one glucoamylase and four α-glucosidases) were regulated by GlnR and PhoP, which are global regulators of nitrogen and phosphate metabolism, respectively. Electrophoretic mobility shift assays and RT-PCR analyses demonstrated that GlnR and PhoP directly interact with their promoter regions, and collaboratively or competitively activate their transcription...
September 16, 2016: Applied and Environmental Microbiology
Jasna Lalić, Melanija Posavec Marjanović, Luca Palazzo, Dragutin Perina, Igor Sabljić, Roko Žaja, Thomas Colby, Bruna Pleše, Mirna Halasz, Gytis Jankevicius, Giselda Bucca, Marijan Ahel, Ivan Matić, Helena Ćetković, Marija Luić, Andreja Mikoč, Ivan Ahel
ADP-ribosylation is a post-translational modification that can alter the physical and chemical properties of target proteins and controls many important cellular processes. Macrodomains are evolutionarily conserved structural domains that bind ADP-ribose derivatives and are found in proteins with diverse cellular functions. Some proteins from the macrodomain family can hydrolyze ADP-ribosylated substrates and therefore reverse this post-translational modification. Bacteria and Streptomyces, in particular, are known to utilize protein ADP-ribosylation, yet very little is known about their enzymes that synthesise and remove this modification...
September 15, 2016: Journal of Biological Chemistry
John T Munnoch, Ma Teresa Pellicer Martinez, Dimitri A Svistunenko, Jason C Crack, Nick E Le Brun, Matthew I Hutchings
Members of the Rrf2 superfamily of transcription factors are widespread in bacteria but their functions are largely unexplored. The few that have been characterized in detail sense nitric oxide (NsrR), iron limitation (RirA), cysteine availability (CymR) and the iron sulfur (Fe-S) cluster status of the cell (IscR). In this study we combined ChIP- and dRNA-seq with in vitro biochemistry to characterize a putative NsrR homologue in Streptomyces venezuelae. ChIP-seq analysis revealed that rather than regulating the nitrosative stress response like Streptomyces coelicolor NsrR, Sven6563 binds to a conserved motif at a different, much larger set of genes with a diverse range of functions, including a number of regulators, genes required for glutamine synthesis, NADH/NAD(P)H metabolism, as well as general DNA/RNA and amino acid/protein turn over...
2016: Scientific Reports
Minsuk Kim, Gwanggyu Sun, Dong-Yup Lee, Byung-Gee Kim
MOTIVATION: Modulation of regulatory circuits governing the metabolic processes is a crucial step for developing microbial cell factories. Despite the prevalence of in silico strain design algorithms, most of them are not capable of predicting required modifications in regulatory networks. Although a few algorithms may predict relevant targets for transcriptional regulator (TR) manipulations, they have limited reliability and applicability due to their high dependency on the availability of integrated metabolic/regulatory models...
September 7, 2016: Bioinformatics
Anyarat Thanapipatsiri, Juan Pablo Gomez-Escribano, Lijiang Song, Maureen Bibb, Mahmoud Al-Bassam, Govind Chandra, Arinthip Thamchaipenet, Greg Challis, Mervyn Bibb
Comparative transcriptional profiling of a ΔbldM mutant of Streptomyces venezuelae with its unmodified progenitor revealed that the expression of a cryptic biosynthetic gene cluster containing both Type I and Type III polyketide synthase genes is activated in the mutant. The 29.5 kb gene cluster, which was predicted to encode an unusual biaryl metabolite, venemycin, and potentially halogenated derivatives, contains 17 genes including one, vemR, encoding a transcriptional activator of the large ATP-binding LuxR-like (LAL) family...
September 8, 2016: Chembiochem: a European Journal of Chemical Biology
Tierrafría Víctor H, Licona-Cassani Cuauhtemoc, Maldonado-Carmona Nidia, Romero-Rodríguez Alba, Centeno-Leija Sara, Marcellin Esteban, Rodríguez-Sanoja Romina, Beatriz Ruiz-Villafán, Nielsen Lars K, Sánchez Sergio
Although the specific function of SCO2127 remains elusive, it has been assumed that this hypothetical protein plays an important role in carbon catabolite regulation and therefore in antibiotic biosynthesis in Streptomyces coelicolor. To shed light on the functional relationship of SCO2127 to the biosynthesis of actinorhodin, a detailed analysis of the proteins differentially produced between the strain M145 and the Δsco2127 mutant of S. coelicolor was performed. The delayed morphological differentiation and impaired production of actinorhodin showed by the deletion strain were accompanied by increased abundance of gluconeogenic enzymes, as well as downregulation of both glycolysis and acetyl-CoA carboxylase...
November 2016: Applied Microbiology and Biotechnology
Joo-Bin Hong, Vijayalakshmi Dhakshnamoorthy, Chang-Ro Lee
The sco0765 gene was annotated as a glycosyl hydrolase family 5 endoglucanase from the genomic sequence of Streptomyces coelicolor A3(2) and consisted of 2,241 bp encoding a polypeptide of 747 amino acids (molecular weight of 80.5 kDa) with a 29-amino acid signal peptide for secretion. The SCO0765 recombinant protein was heterogeneously over-expressed in Streptomyces lividans TK24 under the control of a strong ermE* promoter. The purified SCO0765 protein showed the expected molecular weight of the mature form (718 aa, 77...
September 2016: Journal of Microbiology / the Microbiological Society of Korea
Rafat Amin, Mirita Franz-Wachtel, Yvonne Tiffert, Martin Heberer, Mohamed Meky, Yousra Ahmed, Arne Matthews, Sergii Krysenko, Marco Jakobi, Markus Hinder, Jane Moore, Nicole Okoniewski, Boris Maček, Wolfgang Wohlleben, Agnieszka Bera
Soil-dwelling Streptomyces bacteria such as S.coelicolor have to constantly adapt to the nitrogen (N) availability in their habitat. Thus, strict transcriptional and post-translational control of the N-assimilation is fundamental for survival of this species. GlnR is a global response regulator that controls transcription of the genes related to the N-assimilation in S. coelicolor and other members of the Actinomycetales. GlnR represents an atypical orphan response regulator that is not activated by the phosphorylation of the conserved aspartate residue (Asp 50)...
2016: Frontiers in Molecular Biosciences
Marcin Jan Szafran, Martyna Gongerowska, Paweł Gutkowski, Jolanta Zakrzewska-Czerwińska, Dagmara Jakimowicz
: Maintaining an optimal level of chromosomal supercoiling is critical for the progression of DNA replication and transcription. Moreover, changes in global supercoiling affect the expression of a large number of genes and play a fundamental role in adapting to stress. Topoisomerase I (TopA) and gyrase are key players in the regulation of bacterial chromosomal topology through their respective abilities to relax and compact DNA. Soil bacteria such as Streptomyces species, which grow as branched, multigenomic hyphae, are subject to environmental stresses that are associated with changes in chromosomal topology...
November 1, 2016: Journal of Bacteriology
Tobias Busche, Anika Winkler, Ina Wedderhoff, Christian Rückert, Jörn Kalinowski, Darío Ortiz de Orué Lucana
The secreted protein HbpS, the membrane-embedded sensor kinase SenS and the cytoplasmic response regulator SenR from streptomycetes have been shown to form a novel type of signaling pathway. Based on structural biology as well as different biochemical and biophysical approaches, redox stress-based post-translational modifications in the three proteins were shown to modulate the activity of this signaling pathway. In this study, we show that the homologous system, named here HbpSc-SenSc-SenRc, from the model species Streptomyces coelicolor A3(2) provides this bacterium with an efficient defense mechanism under conditions of oxidative stress...
2016: PloS One
Karl Syson, Clare E M Stevenson, Farzana Miah, J Elaine Barclay, Minhong Tang, Andrii Gorelik, Abdul M Rashid, David M Lawson, Stephen Bornemann
GlgE is a maltosyltranferase involved in α-glucan biosynthesis in bacteria that has been genetically validated as a target for tuberculosis therapies. Crystals of the Mycobacterium tuberculosis enzyme diffract at low resolution so most structural studies have been with the very similar Streptomyces coelicolor GlgE isoform 1. Although the donor binding site for α-1-phosphate had been previously structurally defined, the acceptor site had not. Using mutagenesis, kinetics and protein crystallography of the S...
August 16, 2016: Journal of Biological Chemistry
Paula Yagüe, Joost Willemse, Roman I Koning, Beatriz Rioseras, María T López-García, Nathaly Gonzalez-Quiñonez, Carmen Lopez-Iglesias, Pavel V Shliaha, Adelina Rogowska-Wrzesinska, Abraham J Koster, Ole N Jensen, Gilles P van Wezel, Ángel Manteca
Bacteria of the genus Streptomyces are a model system for bacterial multicellularity. Their mycelial life style involves the formation of long multinucleated hyphae during vegetative growth, with occasional cross-walls separating long compartments. Reproduction occurs by specialized aerial hyphae, which differentiate into chains of uninucleoid spores. While the tubulin-like FtsZ protein is required for the formation of all peptidoglycan-based septa in Streptomyces, canonical divisome-dependent cell division only occurs during sporulation...
2016: Nature Communications
Dörte Falke, Marco Fischer, R Gary Sawers
The saprophytic actinobacterium Streptomyces coelicolor A3(2) requires oxygen for filamentous growth. Surprisingly, the bacterium also synthesizes three active respiratory nitrate reductases (Nar), which are believed to contribute to survival, or general fitness, of the bacterium in soil when oxygen becomes limiting. In this study, we analysed Nar3 and showed that activity of the enzyme is restricted to stationary-phase mycelium of S. coelicolor. Phosphate limitation was shown to be necessary for induction of enzyme synthesis...
September 2016: Microbiology
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