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Streptomyces coelicolor

Alma Botas, Rosario Pérez-Redondo, Antonio Rodríguez-García, Rubén Álvarez-Álvarez, Paula Yagüe, Angel Manteca, Paloma Liras
ArgR is a well-characterized transcriptional repressor controlling the expression of arginine and pyrimidine biosynthetic genes in bacteria. In this work, the biological role of Streptomyces coelicolor ArgR was analyzed by comparing the transcriptomes of S. coelicolor Δ argR and its parental strain, S. coelicolor M145, at five different times over a 66-h period. The effect of S. coelicolor ArgR was more widespread than that of the orthologous protein of Escherichia coli , affecting the expression of 1544 genes along the microarray time series...
2018: Frontiers in Microbiology
Konstantinos C Tsolis, Evridiki-Pandora Tsare, Georgia Orfanoudaki, Tobias Busche, Katerina Kanaki, Reshmi Ramakrishnan, Frederic Rousseau, Joost Schymkowitz, Christian Rückert, Jörn Kalinowski, Jozef Anné, Spyridoula Karamanou, Maria I Klapa, Anastassios Economou
BACKGROUND: Members of the genus Streptomyces are Gram-positive bacteria that are used as important cell factories to produce secondary metabolites and secrete heterologous proteins. They possess some of the largest bacterial genomes and thus proteomes. Understanding their complex proteomes and metabolic regulation will improve any genetic engineering approach. RESULTS: Here, we performed a comprehensive annotation of the subcellular localization of the proteome of Streptomyces lividans TK24 and developed the Subcellular Topology of Polypeptides in Streptomyces database (SToPSdb) to make this information widely accessible...
March 15, 2018: Microbial Cell Factories
María T López-García, Paula Yagüe, Nathaly González-Quiñónez, Beatriz Rioseras, Angel Manteca
Extracytoplasmic function (ECF) sigma factors are a major type of bacterial signal-transducers whose biological functions remain poorly characterized in streptomycetes. In this work we studied SCO4117, a conserved ECF sigma factor from the ECF52 family overexpressed during substrate and aerial mycelium stages. The ECF52 sigma factors harbor, in addition to the ECF sigma factor domain, a zinc finger domain, a transmembrane region, a proline-rich C-terminal extension, and a carbohydrate-binding domain. This class of ECF sigma factors is exclusive to Actinobacteria...
2018: Frontiers in Microbiology
Zhendong Li, Xinqiang Liu, Jingzhi Wang, Ying Wang, Guosong Zheng, Yinhua Lu, Guoping Zhao, Jin Wang
In Streptomyces coelicolor , amtB transcription is promptly regulated by the global nitrogen regulator GlnR. Although the GlnR binding cis -element has been characterized in amtB promoter, consisting of three GlnR boxes of a3-b3 , a1-b1 , and a2-b2 , its role in GlnR-mediated transcriptional regulation remains unclear. Here, we showed that GlnR had different binding affinity against each pair of GlnR binding sites in amtB promoter (i.e., a3-b3 , a1-b1 , and a2-b2 sites), and GlnR was able to bind a3-b3 and a1-b1 , respectively, but not a2-b2 alone...
2018: Frontiers in Microbiology
Marc Biarnes, Chang-Kwon Lee, Takuya Nihira, Rainer Breitling, Eriko Takano
Chemically inducible transcription factors are widely used to control gene expression of synthetic devices. The bacterial quorum sensing system is a popular tool to achieve such control. However, different quorum sensing systems have been found to cross-talk, both between themselves and with the hosts of these devices, and they are leaky by nature. Here we evaluate the potential use of the γ-butyolactone system from Streptomyces coelicolor A3(2) M145 as a complementary regulatory circuit. First, two additional genes responsible for the biosynthesis of γ-butyrolactones were identified in S...
March 6, 2018: ACS Synthetic Biology
Yusuke Sone, Shuto Nakamura, Makoto Sasaki, Fumihito Hasebe, Seung-Young Kim, Nobutaka Funa
1,8-Dihydroxynaphthalene (1,8-DHN) is a key intermediate in the biosynthesis of DHN melanin, which is specific to fungi. In this study, we characterized the enzymatic properties of the gene products of an operon consisting of soceCHS1 , bdsA , and bdsB from the gram-negative bacterium, Sorangium cellulosum Heterologous expression of soceCHS1 , bdsA , and bdsB in Streptomyces coelicolor caused secretion of a dark-brown pigment into the broth. High-performance liquid chromatography analysis of the broth revealed that the recombinant strain produced 1,8-DHN, indicating that the operon encoded a novel enzymatic system for the synthesis of 1,8-DHN...
March 2, 2018: Applied and Environmental Microbiology
Robert Howlett, Katri Anttonen, Nicholas Read, Margaret C M Smith
Actinomycete bacteria use polyprenol phosphate mannose as a lipid linked sugar donor for extra-cytoplasmic glycosyl transferases that transfer mannose to cell envelope polymers, including glycoproteins and glycolipids. We showed recently that strains of Streptomyces coelicolor with mutations in the gene ppm1 encoding polyprenol phosphate mannose synthase were both resistant to phage φC31 and have greatly increased susceptibility to antibiotics that mostly act on cell wall biogenesis. Here we show that mutations in the genes encoding enzymes that act upstream of Ppm1 in the polyprenol phosphate mannose synthesis pathway can also confer phage resistance and antibiotic hyper-susceptibility...
March 1, 2018: Microbiology
Shahira H El-Moslamy
In this report, the local nano-MgO synthesizer strain has been isolated from Ocimum sanctum plant and deposited in GenBank as endophytic Streptomyces coelicolor strain E72. Its intracellular metabolic fraction that contains 7.2 μg/μl of carbohydrate, 6.3 g/l of protein and 5.2 nmol/hr/ml of nitrate reductase used to produce multi-surface shaped nano-MgO with diameter ~25 nm. To the best of our knowledge, this is the first report using statistical nanobiotechnological strategies (Plackett -Burman, Box-Behnken and Taguchi experimental designs) to study and evaluate the endophytic S...
February 28, 2018: Scientific Reports
Fan Huang, Jianli Tang, Lian He, Xuezhi Ding, Shaoya Huang, Youming Zhang, Yunjun Sun, Liqiu Xia
BACKGROUND: Syringolin, synthesized by a mixed non-ribosomal peptide synthetase/polyketide synthetase in Pseudomonas syringae pv. syringae (Pss) B728a, is a novel eukaryotic proteasome inhibitor. Meanwhile, directly modifying large fragments in the PKS/NRPS gene cluster through traditional DNA engineering techniques is very difficult. In this study, we directly cloned the syl gene cluster from Pss B301D-R via Red/ET recombineering to effectively express syringolin in heterologous hosts...
February 26, 2018: Microbial Cell Factories
Arturo Casini, Fang-Yuan Chang, Raissa Eluere, Andrew King, Eric M Young, Quentin M Dudley, Ashty Karim, Katelin Pratt, Cassandra Bristol, Anthony Forget, Amar Ghodasara, Robert Warden-Rothman, Rui Gan, Alexander Cristofaro, Amin Espah Borujeni, Min-Hyung Ryu, Jian Li, Yong Chan Kwon, He Wang, Evangelos Tatsis, Carlos Rodriguez-Lopez, Sarah O'Connor, Marnix H Medema, Michael Fischbach, Michael C Jewett, Christopher A Voigt, D Benjamin Gordon
Centralized facilities for genetic engineering, or "biofoundries," offer the potential to design organisms to address emerging needs in medicine, agriculture, industry, and defense. The field has seen rapid advances in technology, but it is difficult to gauge current capabilities or identify gaps across projects. To this end, our foundry was assessed via a timed "pressure test," in which 3 months were given to build organisms to produce 10 molecules unknown to us in advance. By applying a diversity of new approaches, we produced the desired molecule or a closely related one for 6/10 targets during the performance period, and made advances toward production of the others as well...
February 26, 2018: Journal of the American Chemical Society
Hui-Lei Yu, Tuo Li, Fei-Fei Chen, Xiao-Jing Luo, Aitao Li, Chao Yang, Gao-Wei Zheng, Jian-He Xu
Biocatalytic C-H amination is one of the most challenging tasks. C-H amination reaction can hardly be driven efficiently by solely one enzyme so far. Thus, enzymatic synergy represents an alternative strategy. Herein, we report an "Artificially Bioamination Pathway" for C-H amination of cyclohexane as a model substrate. Three enzymes, a monooxygenase P450BM3 mutant, an alcohol dehydrogenase ScCR from Streptomyces coelicolor and an amine dehydrogenase EsLeuDH from Exiguobactertium sibiricum, constituted a clean cascade reaction system with easy product isolation...
February 22, 2018: Metabolic Engineering
Robert Howlett, Nicholas Read, Anpu Varghese, Charles Kershaw, Y Hancock, Margaret C M Smith
Polyprenol phosphate mannose (PPM) is a lipid-linked sugar donor used by extra-cytoplasmic glycosyl tranferases in bacteria. PPM is synthesiszed by polyprenol phosphate mannose synthase, Ppm1, and in most Actinobacteria is used as the sugar donor for protein O-mannosyl transferase, Pmt, in protein glycosylation. Ppm1 and Pmt have homologues in yeasts and humans, where they are required for protein O-mannosylation. Actinobacteria also use PPM for lipoglycan biosynthesis. Here we show that ppm1 mutants of Streptomyces coelicolor have increased susceptibility to a number of antibiotics that target cell wall biosynthesis...
February 1, 2018: Microbiology
Min Woo Kim, Bo-Rahm Lee, SungYong You, Eun-Jung Kim, Ji-Nu Kim, Eunjung Song, Yung-Hun Yang, Daehee Hwang, Byung-Gee Kim
Most secondary metabolism in Actinobacteria is controlled by multi-layered, gene-regulatory networks. These regulatory mechanisms are not easily identified due to their complexity. As a result, when a strong transcriptional regulator (TR) governs activation of biosynthetic pathways of target antibiotics such as actinorhodin (ACT), additional enhancement of the biosynthesis is difficult in combination with other TRs. To find out any "synergistic transcriptional regulators (sTRs)" that show an additive effect on the major, often strong, transcriptional regulator (mTR), here, we performed a clustering analysis using the transcriptome datasets of an mTR deletion mutant and wild-type strain...
February 17, 2018: Applied Microbiology and Biotechnology
Jessy Mariam, Ruchi Anand
No abstract text is available yet for this article.
2018: Methods in Molecular Biology
Yucong Yu, Biao Tang, Ruixue Dai, Bo Zhang, Liqiang Chen, Hua Yang, Guoping Zhao, Xiaoming Ding
The genus Streptomyces have been highly regarded for their important source of natural products. Combined with the technology of genome sequencing and mining, we could identify the active ingredients from fermentation broth quickly. Here, we report on Streptomyces sp. strain fd1-xmd, which was isolated from a soil sample collected in Shanghai. Interestingly, the fermentation broth derived from this strain demonstrated broad-spectrum antimicrobial activity against gram-positive bacteria, gram-negative bacteria, and eukaryotes...
February 8, 2018: Applied Microbiology and Biotechnology
Marco Di Salvo, Eva Pinatel, Adelfia Talà, Marco Fondi, Clelia Peano, Pietro Alifano
BACKGROUND: Over the last few decades, computational genomics has tremendously contributed to decipher biology from genome sequences and related data. Considerable effort has been devoted to the prediction of transcription promoter and terminator sites that represent the essential "punctuation marks" for DNA transcription. Computational prediction of promoters in prokaryotes is a problem whose solution is far from being determined in computational genomics. The majority of published bacterial promoter prediction tools are based on a consensus-sequences search and they were designed specifically for vegetative σ70 promoters and, therefore, not suitable for promoter prediction in bacteria encoding a lot of σ factors, like actinomycetes...
February 6, 2018: BMC Bioinformatics
Chang-Ro Lee, Won-Jae Chi, Ju-Hyeon Lim, Vijayalakshmi Dhakshnamoorthy, Soon-Kwang Hong
The sco6546 gene of Streptomyces coelicolor A3(2) was annotated as a putative glycosyl hydrolase belonging to family 48. It is predicted to encode a 973-amino acid polypeptide (103.4 kDa) with a 39-amino acid secretion signal. Here, the SCO6546 protein was overexpressed in Streptomyces lividans TK24, and the purified protein showed the expected molecular weight of the mature secreted form (934 aa, 99.4 kDa) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SCO6546 showed high activity toward Avicel and carboxymethyl cellulose, but low activity toward filter paper and β-glucan...
February 5, 2018: Journal of Basic Microbiology
Misaki Ishizuka, Yu Imai, Keiichiro Mukai, Kazuma Shimono, Ryoko Hamauzu, Kozo Ochi, Takeshi Hosaka
Lincomycin forms cross-links within the peptidyl transferase loop region of the 23S ribosomal RNA (rRNA) of the 50S subunit of the bacterial ribosome, which is the site of peptide bond formation, thereby inhibiting protein synthesis. We have previously reported that lincomycin at concentrations below the minimum inhibitory concentration potentiates the production of secondary metabolites in actinomycete strains, suggesting that activation of these strains by utilizing the dose-dependent response of lincomycin could be used to effectively induce the production of cryptic secondary metabolites...
January 25, 2018: Antonie Van Leeuwenhoek
Wei Quan, Chong Zhang, Meixia Zheng, Zhaoxin Lu, Fengxia Lu
BACKGROUND: The effects of Small laccase (SLAC) from Streptomyces coelicolor on the prosperities of whey protein isolate (WPI) film were studied. RESULTS: WPI was catalyze by SLAC without phenolic acid assistance. Particle size distribution results showed that some complexes with higher relative molecular weight formed in WPI samples treated by SLAC. The content of α-helixes decreased, while those of β-sheets and random coils increased following SLAC treatment according to circular dichroism results...
January 24, 2018: Journal of the Science of Food and Agriculture
José L Neira, Felipe Hornos, Concetta Cozza, Ana Cámara-Artigas, Olga Abián, Adrián Velázquez-Campoy
The phosphotransferase system (PTS) controls the preferential use of sugars in bacteria and it is also involved in other processes, such as chemotaxis. It is formed by a protein cascade in which the first two proteins are general (namely, EI and HPr) and the others are sugar-specific permeases. The Rsd protein binds specifically to the RNA polymerase (RNAP) σ70 factor. We first characterized the conformational stability of Escherichia coli Rsd. And second, we delineated the binding regions of Streptomyces coelicolor, HPrsc, and E...
December 26, 2017: Archives of Biochemistry and Biophysics
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