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Streptomyces coelicolor

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https://www.readbyqxmd.com/read/28640671/regulation-of-a-muralytic-enzyme-encoding-gene-by-two-non-coding-rnas
#1
Renée J St-Onge, Marie A Elliot
Non-coding regulatory RNAs fine-tune gene expression post-transcriptionally. In the streptomycetes, rpfA - encoding a muralytic enzyme required for establishing and exiting dormancy - is flanked by non-coding regulatory RNA elements both upstream (riboswitch) and downstream [antisense small RNA (sRNA)]. In Streptomyces coelicolor, the upstream riboswitch decreases rpfA transcript abundance in response to the second messenger cyclic di-AMP, itself involved in cell wall metabolism and dormancy. There is, however, no obvious expression platform associated with this riboswitch and consequently, its mechanism of action is entirely unknown...
June 22, 2017: RNA Biology
https://www.readbyqxmd.com/read/28631385/identification-of-glucose-kinase-dependent-and-independent-pathways-for-carbon-control-of-primary-metabolism-development-and-antibiotic-production-in-streptomyces-coelicolor-by-quantitative-proteomics
#2
Jacob Gubbens, Marleen M Janus, Bogdan I Florea, Herman S Overkleeft, Gilles P van Wezel
No abstract text is available yet for this article.
July 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28601893/characterization-of-an-lrp-asnc-family-regulator-sco3361-controlling-actinorhodin-production-and-morphological-development-in-streptomyces-coelicolor
#3
Jing Liu, Jie Li, Hong Dong, Yunfu Chen, Yansheng Wang, Hang Wu, Changrun Li, David T Weaver, Lixin Zhang, Buchang Zhang
Lrp/AsnC family regulators have been found in many bacteria as crucial regulators controlling diverse cellular processes. By genomic alignment, we found that SCO3361, an Lrp/AsnC family protein from Streptomyces coelicolor, shared the highest similarity to the SACE_Lrp from Saccharopolyspora erythraea. Deletion of SCO3361 led to dramatic reduction in actinorhodin (Act) production and delay in aerial mycelium formation and sporulation on solid media. Dissection of the mechanism underlying the function of SCO3361 in Act production revealed that it altered the transcription of the cluster-situated regulator gene actII-ORF4 by directly binding to its promoter...
June 11, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28599184/plasma-modified-pla-electrospun-membranes-for-actinorhodin-production-intensification-in-streptomyces-coelicolor-immobilized-cell-cultivations
#4
Roberto Scaffaro, Francesco Lopresti, Alberto Sutera, Luigi Botta, Rosa Maria Fontana, Giuseppe Gallo
Most of industrially relevant bioproducts are produced by submerged cultivations of actinomycetes. The immobilization of these Gram-positive filamentous bacteria on suitable porous supports may prevent mycelial cell-cell aggregation and pellet formation which usually negatively affect actinomycete submerged cultivations, thus, resulting in an improved biosynthetic capability. In this work, electrospun polylactic acid (PLA) membranes, subjected or not to O2-plasma treatment (PLA-plasma), were used as support for immobilized-cell submerged cultivations of Streptomyces coelicolor M145...
May 31, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28598435/zinc-dependent-regulation-of-zinc-import-and-export-genes-by-zur
#5
Seung-Hwan Choi, Kang-Lok Lee, Jung-Ho Shin, Yoo-Bok Cho, Sun-Shin Cha, Jung-Hye Roe
In most bacteria, zinc depletion is sensed by Zur, whereas the surplus is sensed by different regulators to achieve zinc homeostasis. Here we present evidence that zinc-bound Zur not only represses genes for zinc acquisition but also induces the zitB gene encoding a zinc exporter in Streptomyces coelicolor, a model actinobacteria. Zinc-dependent gene regulation by Zur occurs in two phases. At sub-femtomolar zinc concentrations (phase I), dimeric Zur binds to the Zur-box motif immediately upstream of the zitB promoter, resulting in low zitB expression...
June 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/28569150/heterologous-expression-of-pikromycin-biosynthetic-gene-cluster-using-streptomyces-artificial-chromosome-system
#6
Hye-Rim Pyeon, Hee-Ju Nah, Seung-Hoon Kang, Si-Sun Choi, Eung-Soo Kim
BACKGROUND: Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully applied for precise cloning and tandem integration of a large polyketide tautomycetin (TMC) biosynthetic gene cluster (Nah et al. in Microb Cell Fact 14(1):1, 2015), implying that this strategy could be employed to develop a custom overexpression scheme of natural product pathway clusters present in actinomycetes...
May 31, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28560880/quantitative-proteomics-analysis-confirmed-oxidative-metabolism-predominates-in-streptomyces-coelicolor-versus-glycolytic-metabolism-in-streptomyces-lividans
#7
Aaron Millan-Oropeza, Céline Henry, Mélisande Blein-Nicolas, Anne Aubert-Frambourg, Fathi Moussa, Jean Bleton, Marie-Jöelle Virolle
Recent physiological studies indicated that S. lividans metabolism was mainly glycolytic, whereas S. coelicolor metabolism was mainly oxidative. To determine whether such metabolic characteristics were correlated with consistent proteomics features, a comparative label-free, shotgun proteomics analysis of these strains was carried out. Among 2024 proteins identified, 360 showed significant differences in abundance between the strains. This study revealed that S. coelicolor catabolized glucose less actively than S...
June 15, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28553520/watasemycin-biosynthesis-in-streptomyces-venezuelae-thiazoline-c-methylation-by-a-type-b-radical-sam-methylase-homologue
#8
Yuki Inahashi, Shanshan Zhou, Maureen J Bibb, Lijiang Song, Mahmoud M Al-Bassam, Mervyn J Bibb, Gregory L Challis
2-Hydroxyphenylthiazolines are a family of iron-chelating nonribosomal peptide natural products that function as virulence-conferring siderophores in various Gram-negative bacteria. They have also been reported as metabolites of Gram-positive Streptomyces species. Transcriptional analyses of Streptomyces venezuelae ATCC 10712 revealed that its genome contains a putative 2-hydroxyphenylthiazoline biosynthetic gene cluster. Heterologous expression of the gene cluster in Streptomyces coelicolor M1152 showed that the mono- and dimethylated derivatives, thiazostatin and watasemycin, respectively, of the 2-hydroxyphenylthiazoline enantiopyochelin are two of its metabolic products...
April 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28536705/role-of-gntr-family-regulatory-gene-sco1678-in-gluconate-metabolism-in-streptomyces-coelicolor-m145
#9
Olga Tsypik, Roman Makitrynskyy, Agnieszka Bera, Lijiang Song, Wolfgang Wohlleben, Victor Fedorenko, Bohdan Ostash
Here we report functional characterization of the Streptomyces coelicolor M145 gene SCO1678, which encodes a GntR-like regulator of the FadR subfamily. Bioinformatic analysis suggested that SCO1678 is part of putative operon (gnt) involved in gluconate metabolism. Combining the results of SCO1678 knockout, transcriptional analysis of gnt operon, and Sco1678 protein-DNA electromobility shift assays, we established that Sco1678 protein controls the gluconate operon. It does so via repression of its transcription from a single promoter located between genes SCO1678 and SCO1679...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28487688/gamma-glutamylpolyamine-synthetase-glna3-is-involved-in-the-first-step-of-polyamine-degradation-pathway-in-streptomyces-coelicolor-m145
#10
Sergii Krysenko, Nicole Okoniewski, Andreas Kulik, Arne Matthews, Jan Grimpo, Wolfgang Wohlleben, Agnieszka Bera
Streptomyces coelicolor M145 was shown to be able to grow in the presence of high concentrations of polyamines, such as putrescine, cadaverine, spermidine, or spermine, as a sole nitrogen source. However, hardly anything is known about polyamine utilization and its regulation in streptomycetes. In this study, we demonstrated that only one of the three proteins annotated as glutamine synthetase-like protein, GlnA3 (SCO6962), was involved in the catabolism of polyamines. Transcriptional analysis revealed that the expression of glnA3 was strongly induced by exogenous polyamines and repressed in the presence of ammonium...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28484446/an-alternative-%C3%AF-factor-%C3%AF-8-controls-avermectin-production-and-multiple-stress-responses-in-streptomyces-avermitilis
#11
Di Sun, Qian Wang, Zhi Chen, Jilun Li, Ying Wen
Alternative σ factors in bacteria redirect RNA polymerase to recognize alternative promoters, thereby facilitating coordinated gene expression necessary for adaptive responses. The gene sig8 (sav_741) in Streptomyces avermitilis encodes an alternative σ factor, σ(8), highly homologous to σ(B) in Streptomyces coelicolor. Studies reported here demonstrate that σ(8) is an important regulator of both avermectin production and stress responses in S. avermitilis. σ(8) inhibited avermectin production by indirectly repressing expression of cluster-situated activator gene aveR, and by directly initiating transcription of its downstream gene sav_742, which encodes a direct repressor of ave structural genes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28463421/aromatic-polyketide-gtri-02-is-a-previously-unidentified-product-of-the-act-gene-cluster-in-streptomyces-coelicolor%C3%A2-a3-2
#12
Changsheng Wu, Koji Ichinose, Young Hae Choi, Gilles P van Wezel
The biosynthesis of aromatic polyketides derived from type II polyketide synthases (PKSs) is complex, and it is not uncommon that highly similar gene clusters give rise to diverse structural architectures. The act biosynthetic gene cluster (BGC) of the model actinomycete Streptomyces coelicolor A3(2) is an archetypal type II PKS. Here we show that the act BGC also specifies the aromatic polyketide GTRI-02 (1) and propose a mechanism for the biogenesis of its 3,4-dihydronaphthalen-1(2H)-one backbone. Polyketide 1 was also produced by Streptomyces sp...
April 30, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28461451/a-novel-two-component-system-glur-k-involved-in-glutamate-sensing-and-uptake-in-streptomyces-coelicolor
#13
Lei Li, Weihong Jiang, Yinhua Lu
Two-component systems (TCSs), the predominant signal transduction pathways employed by bacteria, play important roles in physiological metabolism in Streptomyces Here, a novel TCS GluR-K (encoded by SCO5778-5779), which is located divergently from the gluABCD operon encoding a glutamate uptake system, was identified being involved in glutamate sensing and uptake as well as antibiotic biosynthesis in Streptomyces coelicolor Under the minimal medium (MM) supplemented with different concentrations of glutamate, deletion of gluR-K resulted in enhanced ACT but reduced RED and yCPK production, suggesting GluR-K plays a differential role in antibiotic biosynthesis...
May 1, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28455615/enhancement-of-antibiotic-productions-by-engineered-nitrate-utilization-in-actinomycetes
#14
Sitong Meng, Hang Wu, Lei Wang, Buchang Zhang, Linquan Bai
Nitrate is necessary for primary and secondary metabolism of actinomycetes and stimulates the production of a few antibiotics, such as lincomycin and rifamycin. However, the mechanism of this nitrate-stimulating effect was not fully understood. Two putative ABC-type nitrate transporters were identified in Streptomyces lincolnensis NRRL2936 and verified to be involved in lincomycin biosynthesis. With nitrate supplementation, the transcription of nitrogen assimilation genes, nitrate-specific ABC1 transporter genes, and lincomycin exporter gene lmrA was found to be enhanced and positively regulated by the global regulator GlnR, whose expression was also improved...
April 28, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28455590/crystal-structure-of-streptomyces-coelicolor-rraas2-an-unusual-member-of-the-rnase-e-inhibitor-rraa-protein-family
#15
Nohra Park, Jihune Heo, Saemee Song, Inseong Jo, Kangseok Lee, Nam-Chul Ha
Bacterial ribonuclease E (RNase E) plays a crucial role in the processing and decay of RNAs. A small protein named RraA negatively regulates the activity of RNase E via protein-protein interaction in various bacteria. Recently, RraAS1 and RraAS2, which are functional homologs of RraA from Escherichia coli, were identified in the Gram-positive species Streptomyces coelicolor. RraAS1 and RraAS2 inhibit RNase ES ribonuclease activity in S. coelicolor. RraAS1 and RraAS2 have a C-terminal extension region unlike typical bacterial RraA proteins...
May 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28451186/thioester-reduction-and-aldehyde-transamination-are-universal-steps-in-actinobacterial-polyketide-alkaloid-biosynthesis
#16
U R Awodi, J L Ronan, J Masschelein, E L C de Los Santos, G L Challis
Actinobacteria produce a variety of polyketide alkaloids with unusual structures. Recently, it was shown that a type I modular polyketide synthase (PKS) is involved in the assembly of coelimycin P1, a polyketide alkaloid produced by Streptomyces coelicolor M145. However, the mechanisms for converting the product of the PKS to coelimycin P1 remain to be elucidated. Here we show that the C-terminal thioester reductase (TR) domain of the PKS and an ω-transaminase are responsible for release of the polyketide chain as an aldehyde and its subsequent reductive amination...
January 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28439624/evidence-for-the-formation-of-scbr-scbr2-heterodimers-and-identification-of-one-of-the-regulatory-targets-in-streptomyces-coelicolor
#17
Xiao Li, Juan Wang, Mingxin Shi, Weishan Wang, Christophe Corre, Keqian Yang
The homologous transcriptional regulators ScbR and ScbR2 have previously been identified as γ-butyrolactone (GBL) and antibiotic receptors, respectively. They regulate diverse physiological processes in Streptomyces coelicolor in response to GBL and antibiotic signals. In this study, ScbR and ScbR2 proteins were shown to interact using a bacterial two-hybrid system where adenylate cyclase activity was reconstituted in Escherichia coli BTH101. These ScbR/ScbR2 interactions in S. coelicolor were then demonstrated by co-immunoprecipitation...
April 24, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28425466/crystal-structures-of-the-no-sensor-nsrr-reveal-how-its-iron-sulfur-cluster-modulates-dna-binding
#18
Anne Volbeda, Erin L Dodd, Claudine Darnault, Jason C Crack, Oriane Renoux, Matthew I Hutchings, Nick E Le Brun, Juan C Fontecilla-Camps
NsrR from Streptomyces coelicolor (Sc) regulates the expression of three genes through the progressive degradation of its [4Fe-4S] cluster on nitric oxide (NO) exposure. We report the 1.95 Å resolution crystal structure of dimeric holo-ScNsrR and show that the cluster is coordinated by the three invariant Cys residues from one monomer and, unexpectedly, Asp8 from the other. A cavity map suggests that NO displaces Asp8 as a cluster ligand and, while D8A and D8C variants remain NO sensitive, DNA binding is affected...
April 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28422240/zwitterionic-pyrrolidene-phosphonates-inhibitors-of-the-glycoside-hydrolase-like-phosphorylase-streptomyces-coelicolor-glgei-v279s
#19
Sri Kumar Veleti, Cecile Petit, Donald R Ronning, Steven J Sucheck
We synthesized and evaluated new zwitterionic inhibitors against glycoside hydrolase-like phosphorylase Streptomyces coelicolor (Sco) GlgEI-V279S which plays a role in α-glucan biosynthesis. Sco GlgEI-V279S serves as a model enzyme for validated anti-tuberculosis (TB) target Mycobacterium tuberculosis (Mtb) GlgE. Pyrrolidine inhibitors 5 and 6 were designed based on transition state considerations and incorporate a phosphonate on the pyrrolidine moiety to expand the interaction network between the inhibitor and the enzyme active site...
May 10, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28400129/the-ph-dependent-assembly-of-chaplin-e-from-streptomyces-coelicolor
#20
Mina Dokouhaki, Andrew Hung, Li Day, Sally L Gras
Chaplin E, is one of five self-assembling peptides secreted by Streptomyces coelicolor that assist aerial growth by lowering the surface tension of water. Although the surface activity of a mixture of chaplin peptides has observed to depend on pH, it is unclear how the solvent environment (i.e. pH) influences the structure, assembly and subsequent functionality of these individual peptides. In this study, the conformation and fibril forming propensity of the Chaplin E peptide was assessed as a function of pH using a combination of experimental measurements and molecular dynamics simulations...
April 8, 2017: Journal of Structural Biology
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