keyword
https://read.qxmd.com/read/38479473/heterologous-production-of-geosmin-in-saccharomyces-cerevisiae
#1
JOURNAL ARTICLE
Dheeraj Madhukar Mahajan, Pramod Shankar Kumbhar, Rishi Jain
(-)-Geosmin has high demand in perfumes and cosmetic products for its earthy congenial aroma. The current production of (-)-geosmin is either by distillation of sun-baked soil or by inefficient chemical synthesis because of the presence of multiple chiral centers. Fermentation processes are not viable as the titers of the Streptomyces sp. based processes are low. This work presents an alternative route by the heterologous synthesis of (-)-geosmin in Saccharomyces cerevisiae. The enzyme involved is the bifunctional geosmin synthase that catalyzes the conversion of farnesyl diphosphate to germacradienol and germacradienol to geosmin...
March 11, 2024: Journal of Biotechnology
https://read.qxmd.com/read/38447069/engineering-site-228-of-streptomyces-coelicolor-laccase-for-optimizing-catalytic-activity
#2
JOURNAL ARTICLE
Luyao Bian, Silu Zhang, Tingting Chang, Jiacheng Zhang, Chong Zhang
Malachite green (MG) poses a formidable threat to ecosystems and human health. Laccase emerges as a promising candidate for MG degradation, prompting an investigation into the catalytic activity modulation of a small laccase (SLAC) from Streptomyces coelicolor , with a focus on amino acid position 228. Through saturation mutagenesis, five mutants with a 50% increase in the specific activity were generated. Characterization revealed notable properties, K m of E228F was 8.8% of the wild type (WT), and E288T exhibited a 133% k cat compared to WT...
March 6, 2024: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/38433158/actinobacterial-peroxidase-mediated-biodeterioration-of-hazardous-explosive-2-4-6-trinitrophenol-by-in-silico-and-in-vitro-approaches
#3
JOURNAL ARTICLE
Prasath Sai Preethi, N M Hariharan, Shanmugam Dilip Kumar, Manian Rameshpathy, Ramasamy Subbaiya, Natchimuthu Karmegam
Explosives are perilous and noxious to aquatic biota disrupting their endocrinal systems. Supplementarily, they exhibit carcinogenic, teratogenic and mutagenic effects on humans and animals. Henceforth, the current study has been targeted to biotransform the explosive, 2, 4, 6 trinitrophenol (TNP) by wetland peroxidase from Streptomyces coelicolor. A total peroxidase yield of 20,779 mg/l with 51.6 folds of purification was observed. In silico molecular docking cum in vitro appraisals were accomplished to assess binding energy and interacting binding site residues of peroxidase and TNP complex...
March 4, 2024: Environmental Geochemistry and Health
https://read.qxmd.com/read/38427710/structural-and-functional-characterization-of-afsr-an-sarp-family-transcriptional-activator-of-antibiotic-biosynthesis-in-streptomyces
#4
JOURNAL ARTICLE
Yiqun Wang, Xu Yang, Feng Yu, Zixin Deng, Shuangjun Lin, Jianting Zheng
Streptomyces antibiotic regulatory proteins (SARPs) are widely distributed activators of antibiotic biosynthesis. Streptomyces coelicolor AfsR is an SARP regulator with an additional nucleotide-binding oligomerization domain (NOD) and a tetratricopeptide repeat (TPR) domain. Here, we present cryo-electron microscopy (cryo-EM) structures and in vitro assays to demonstrate how the SARP domain activates transcription and how it is modulated by NOD and TPR domains. The structures of transcription initiation complexes (TICs) show that the SARP domain forms a side-by-side dimer to simultaneously engage the afs box overlapping the -35 element and the σHrdB region 4 (R4), resembling a sigma adaptation mechanism...
March 1, 2024: PLoS Biology
https://read.qxmd.com/read/38427033/low-toxicity-and-high-efficiency-streptomyces-genome-editing-tool-based-on-the-miniature-type-v-f-crispr-cas-nuclease-ascas12f1
#5
JOURNAL ARTICLE
Hui-Min Hua, Jia-Feng Xu, Xue-Shuang Huang, Andrei A Zimin, Wen-Fang Wang, Yin-Hua Lu
Genome editing tools based on SpCas9 and FnCpf1 have facilitated strain improvements for natural product production and novel drug discovery in Streptomyces . However, due to high toxicity, their editing requires high DNA transformation efficiency, which is unavailable in most streptomycetes. The transformation efficiency of an all-in-one editing tool based on miniature Cas nuclease AsCas12f1 was significantly higher than those of SpCas9 and FnCpf1 in tested streptomycetes, which is due to its small size and weak DNA cleavage activity...
March 1, 2024: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/38420671/structure-based-engineering-of-a-sesquiterpene-cyclase-to-generate-an-alcohol-product-conversion-of-epi-isozizaene-synthase-into-%C3%AE-bisabolol-synthase
#6
JOURNAL ARTICLE
Samuel A Eaton, David W Christianson
The sesquiterpene cyclase epi -isozizaene synthase (EIZS) from Streptomyces coelicolor catalyzes the metal-dependent conversion of farnesyl diphosphate (FPP) into the complex tricyclic product epi -isozizaene. This remarkable transformation is governed by an active site contour that serves as a template for catalysis, directing the conformations of multiple carbocation intermediates leading to the final product. Mutagenesis of residues defining the active site contour remolds its three-dimensional shape and reprograms the cyclization cascade to generate alternative cyclization products...
February 29, 2024: Biochemistry
https://read.qxmd.com/read/38403403/secretory-production-and-characterization-of-a-highly-effective-chitosanase-from-streptomyces-coelicolor-a3-2-m145-in-pichia-pastoris
#7
JOURNAL ARTICLE
Jiayu Liang, Shengbin He, Jian Sun, Haodong Bao, Lanyu Cui
In this study, a glycoside hydrolase family 46 chitosanase from Streptomyces coelicolor A3(2) M145 was firstly cloned and expressed in Pichia pastoris GS115 (P. pastoris GS115). The recombinant enzyme (CsnA) showed maximal activity at pH 6.0 and 65°C. Both thermal stability and pH stability of CsnA expressed in P. pastoris GS115 were significantly increased compared with homologous expression in Streptomyces coelicolor A3(2). A stable chitosanase activity of 725.7 ± 9.58 U mL-1 was obtained in fed-batch fermentation...
February 2024: Biotechnology Journal
https://read.qxmd.com/read/38389542/insights-into-additional-lactone-based-signaling-circuits-in-streptomyces-existence-of-acyl-homoserine-lactones-and-luxi-luxr-homologs-in-six-streptomyces-species
#8
JOURNAL ARTICLE
Amir Salehi-Najafabadi, Sepand Tehrani Fateh, Ghasem Amoabediny, Javad Hamedi
Acyl-homoserine lactones (AHLs), mediating pivotal physiological activities through quorum sensing (QS), have conventionally been considered limited to Gram-negative bacteria. However, few reports on the existence of AHLs in Gram-positive bacteria have questioned this conception. Streptomyces , as Gram-positive bacteria already utilizing a lactone-based QS molecule (i.e., gamma-butyrolactones), are yet to be explored for producing AHLs, considering their metabolic capacity and physiological distinction. In this regard, our study examined the potential production of AHLs within Streptomyces by deploying HPLC-MS/MS methods, which resulted in the discovery of multiple AHL productions by S...
2024: Frontiers in Microbiology
https://read.qxmd.com/read/38353530/the-ssgb-gene-is-required-for-the-early-stages-of-sporangium-formation-in-actinoplanes-missouriensis
#9
JOURNAL ARTICLE
Takuya Akutsu, Takeaki Tezuka, Manato Maruko, Aiko Hirata, Yasuo Ohnishi
In Streptomyces , multiple paralogs of SsgA-like proteins (SALPs) are involved in spore formation from aerial hyphae. However, the functions of SALPs have not yet been elucidated in other actinobacterial genera. Here, we report the primary function of an SsgB ortholog (AmSsgB) in Actinoplanes missouriensis , which develops terminal sporangia on the substrate mycelia via short sporangiophores. Importantly, AmSsgB is the sole SALP in A. missouriensis . The transcription of AmssgB was upregulated during sporangium formation, consistent with our previous findings that AmssgB is a member of the AmBldD regulon...
February 14, 2024: Journal of Bacteriology
https://read.qxmd.com/read/38349033/activation-of-zinc-uptake-regulator-by-zinc-binding-to-three-regulatory-sites
#10
JOURNAL ARTICLE
Yunchan Choi, Junseock Koh, Sun-Shin Cha, Jung-Hye Roe
Zur is a Fur-family metalloregulator that is widely used to control zinc homeostasis in bacteria. In Streptomyces coelicolor, Zur (ScZur) acts as both a repressor for zinc uptake (znuA) gene and an activator for zinc exporter (zitB) gene. Previous structural studies revealed three zinc ions specifically bound per ScZur monomer; a structural one to allow dimeric architecture and two regulatory ones for DNA-binding activity. In this study, we present evidence that Zur contains a fourth specific zinc-binding site with a key histidine residue (H36), widely conserved among actinobacteria, for regulatory function...
February 13, 2024: Nucleic Acids Research
https://read.qxmd.com/read/38348908/rip-seq-reveals-rnas-that-interact-with-rna-polymerase-and-primary-sigma-factors-in-bacteria
#11
JOURNAL ARTICLE
Viola Vaňková Hausnerová, Mahmoud Shoman, Dilip Kumar, Marek Schwarz, Martin Modrák, Jitka Jirát Matějčková, Eliška Mikesková, Silvia Neva, Anna Herrmannová, Michaela Šiková, Petr Halada, Iva Novotná, Petr Pajer, Leoš Shivaya Valášek, Martin Převorovský, Libor Krásný, Jarmila Hnilicová
Bacteria have evolved structured RNAs that can associate with RNA polymerase (RNAP). Two of them have been known so far-6S RNA and Ms1 RNA but it is unclear if any other types of RNAs binding to RNAP exist in bacteria. To identify all RNAs interacting with RNAP and the primary σ factors, we have established and performed native RIP-seq in Bacillus subtilis, Corynebacterium glutamicum, Streptomyces coelicolor, Mycobacterium smegmatis and the pathogenic Mycobacterium tuberculosis. Besides known 6S RNAs in B...
February 13, 2024: Nucleic Acids Research
https://read.qxmd.com/read/38340355/biosynthesis-of-the-azoxy-compound-azodyrecin-from-streptomyces-mirabilis-p8-a2
#12
JOURNAL ARTICLE
Matiss Maleckis, Mario Wibowo, Tetiana Gren, Scott A Jarmusch, Eva B Sterndorff, Thomas Booth, Nathalie N S E Henriksen, Christopher M Whitford, Xinglin Jiang, Tue S Jørgensen, Ling Ding, Tilmann Weber
Azoxy compounds are a distinctive group of bioactive secondary metabolites characterized by a unique RN═N+ (O- )R moiety. The azoxy moiety is present in various classes of metabolites that exhibit various biological activities. The enzymatic mechanisms underlying azoxy bond formation remain enigmatic. Azodyrecins are cytotoxic azoxy metabolites produced by Streptomyces mirabilis P8-A2. Here, we cloned and confirmed the putative azd biosynthetic gene cluster through CATCH cloning followed by expression and production of azodyrecins in two heterologous hosts, S...
February 10, 2024: ACS Chemical Biology
https://read.qxmd.com/read/38323847/biosynthesis-of-novel-desferrioxamine-derivatives-requires-unprecedented-crosstalk-between-separate-nrps-independent-siderophore-pathways
#13
JOURNAL ARTICLE
Li Su, Yaouba Souaibou, Laurence Hôtel, Cédric Paris, Kira J Weissman, Bertrand Aigle
Iron is essential to many biological processes but its poor solubility in aerobic environments restricts its bioavailability. To overcome this limitation, bacteria have evolved a variety of strategies, including the production and secretion of iron-chelating siderophores. Here, we describe the discovery of four series of siderophores from Streptomyces ambofaciens ATCC23877, three of which are unprecedented. MS/MS-based molecular networking revealed that one of these series corresponds to acylated desferrioxamines (acyl-DFOs) recently identified from S...
February 7, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38289644/construction-and-characterisation-of-a-structured-tuneable-and-transparent-3d-culture-platform-for-soil-bacteria
#14
JOURNAL ARTICLE
Liam M Rooney, Lionel X Dupuy, Paul A Hoskisson, Gail McConnell
We have developed a tuneable workflow for the study of soil microbes in an imitative 3D soil environment that is compatible with routine and advanced optical imaging, is chemically customisable, and is reliably refractive index matched based on the carbon catabolism of the study organism. We demonstrate our transparent soil pipeline with two representative soil organisms, Bacillus subtilis and Streptomyces coelicolor , and visualise their colonisation behaviours using fluorescence microscopy and mesoscopy. This spatially structured, 3D approach to microbial culture has the potential to further study the behaviour of bacteria in conditions matching their native environment and could be expanded to study microbial interactions, such as competition and warfare...
January 2024: Microbiology
https://read.qxmd.com/read/38284010/dye-decolorizing-peroxidase-of-streptomyces-coelicolor-sc-dypb-exists-as-a-dynamic-mixture-of-kinetically-different-oligomers
#15
JOURNAL ARTICLE
Hegne Pupart, Darja Vastšjonok, Tiit Lukk, Priit Väljamäe
Dye-decolorizing peroxidases (DyPs) are heme-dependent enzymes that catalyze the oxidation of various substrates including environmental pollutants such as azo dyes and also lignin. DyPs often display complex non-Michaelis-Menten kinetics with substrate inhibition or positive cooperativity. Here, we performed in-depth kinetic characterization of the DyP of the bacterium Streptomyces coelicolor ( Sc DyPB). The activity of Sc DyPB was found to be dependent on its concentration in the working stock used to initiate the reactions as well as on the pH of the working stock...
January 23, 2024: ACS Omega
https://read.qxmd.com/read/38199083/streptomyces-based-whole-cell-biosensors-for-detecting-diverse-cell-envelope-targeting-antibiotics
#16
JOURNAL ARTICLE
Hengyu Wang, Yong Sheng, Yixin Ou, Min Xu, Meifeng Tao, Shuangjun Lin, Zixin Deng, Linquan Bai, Wei Ding, Qianjin Kang
Cell envelope-targeting antibiotics are potent therapeutic agents against various bacterial infections. The emergence of multiple antibiotic-resistant strains underscores the significance of identifying potent antimicrobials specifically targeting the cell envelope. However, current drug screening approaches are tedious and lack sufficient specificity and sensitivity, warranting the development of more efficient methods. Genetic circuit-based whole-cell biosensors hold great promise for targeted drug discovery from natural products...
January 5, 2024: Biosensors & Bioelectronics
https://read.qxmd.com/read/38184746/%C3%AF-e-of-streptomyces-coelicolor-can-function-both-as-a-direct-activator-or-repressor-of-transcription
#17
JOURNAL ARTICLE
Jiří Pospíšil, Marek Schwarz, Alice Ziková, Dragana Vítovská, Miluše Hradilová, Michal Kolář, Alena Křenková, Martin Hubálek, Libor Krásný, Jiří Vohradský
σ factors are considered as positive regulators of gene expression. Here we reveal the opposite, inhibitory role of these proteins. We used a combination of molecular biology methods and computational modeling to analyze the regulatory activity of the extracytoplasmic σE factor from Streptomyces coelicolor. The direct activator/repressor function of σE was then explored by experimental analysis of selected promoter regions in vivo. Additionally, the σE interactome was defined. Taken together, the results characterize σE , its regulation, regulon, and suggest its direct inhibitory function (as a repressor) in gene expression, a phenomenon that may be common also to other σ factors and organisms...
January 6, 2024: Communications Biology
https://read.qxmd.com/read/38176366/probing-the-mechanism-of-the-dedicated-no-sensor-4fe-4s-nsrr-the-effect-of-cluster-ligand-environment
#18
JOURNAL ARTICLE
Erin L Dodd, Nick E Le Brun
NsrR from Streptomyces coelicolor is a bacterial nitric oxide (NO) sensor/nitrosative stress regulator as its primary function, and has been shown to have differential response at low, mid, and high levels of NO. These must correspond to discrete structural changes at the protein-bound [4Fe-4S] cluster in response to stepwise nitrosylation of the cluster. We have investigated the effect of the monohapto carboxylate ligand in the site differentiated [4Fe-4S] cluster cofactor of the protein NsrR on modulating its reactivity to NO with a focus on indentifying mechanistic intermediates...
December 21, 2023: Journal of Inorganic Biochemistry
https://read.qxmd.com/read/38159786/the-stringent-response-is-strongly-activated-in-the-antibiotic-producing-strain-streptomyces-coelicolor
#19
JOURNAL ARTICLE
Clara Lejeune, David Cornu, Laila Sago, Virginie Redeker, Marie-Joelle Virolle
S. lividans and S. coelicolor are phylogenetically closely related strains with different abilities to produce the same specialized metabolites. Previous studies revealed that the strong antibiotic producer, S. coelicolor, had a lower ability to assimilate nitrogen and phosphate than the weak producer, S. lividans, and this resulted into a lower growth rate. A comparative proteomic dataset was used to establish the consequences of these nutritional stresses on the abundance of proteins of the translational apparatus of these strains, grown in low and high phosphate availability...
December 28, 2023: Research in Microbiology
https://read.qxmd.com/read/38151830/structural-and-biochemical-analysis-of-3-dehydroquinate-dehydratase-from-corynebacterium-glutamicum
#20
JOURNAL ARTICLE
Chan Hwi Lee, Sangwoo Kim, Hogyun Seo, Kyung-Jin Kim
Dehydroquinate dehydratase (DHQD) catalyzes the conversion of 3-dehydroquinic acid (DHQ) into 3-dehydroshikimic acid in the mid stage of the shikimate pathway, which is essential for the biosynthesis of aromatic amino acids and folates. Here, we report two the crystal structures of type II DHQD ( Cg DHQD) derived from Corynebacterium glutamicum , which is a widely used industrial platform organism. We determined the structures for Cg DHQDWT with the citrate at a resolution of 1.80Å and Cg DHQDR19A with DHQ complexed forms at a resolution of 2...
December 28, 2023: Journal of Microbiology and Biotechnology
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