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Streptomyces coelicolor

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https://www.readbyqxmd.com/read/27919888/identification-of-a-novel-lincomycin-resistance-mutation-associated-with-activation-of-antibiotic-production-in-streptomyces-coelicolor-a3-2
#1
Guojun Wang, Masumi Izawa, Xiaoge Yang, Dongbo Xu, Yukinori Tanaka, Kozo Ochi
Comparative genome sequencing analysis of a lincomycin-resistant strain of Streptomyces coelicolor A3 (2) and the wild type strain identified a novel mutation conferring a high level of lincomycin resistance. Surprisingly, the new mutation was an in-frame DNA deletion in the genes SCO4597-SCO4598, resulting in formation of the hybrid gene linR SCO4597 and SCO4598 encode two histidine kinases, which, together with SCO4596, encoding a response regulator, constitute a unique two-component system. Sequence analysis indicated that these three genes and their arrangement patterns are ubiquitous among all Streptomyces genomes sequenced to date, suggesting these genes play important regulatory roles...
December 5, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27902442/the-whcd-gene-of-corynebacterium-glutamicum-plays-roles-in-cell-division-and-envelope-formation
#2
Dong-Seok Lee, Younhee Kim, Heung-Shick Lee
In this study, we analyzed the whcD gene from Corynebacterium glutamicum, which encodes a homolog of whiB, a Streptomyces coelicolor gene required for the sporulation of aerial hyphae. Deletion of the gene (ΔwhcD) severely affected cell growth in C. glutamicum. The ΔwhcD strain exhibited a large, filamentous, branched, and bud-shaped morphology with multiple septa. The transcription levels of the cell division genes involved in Z-ring assembly and septal peptidoglycan synthesis, including ftsZ, sepF, ftsQ, and ftsI, were markedly decreased in the ΔwhcD strain...
November 24, 2016: Microbiology
https://www.readbyqxmd.com/read/27897367/bifunctionality-of-activ-as-a-cyclase-thioesterase-revealed-by-in-vitro-reconstitution-of-actinorhodin-biosynthesis-in-streptomyces-coelicolor-a3-2
#3
Takaaki Taguchi, Takayoshi Awakawa, Yukitaka Nishihara, Michiho Kawamura, Yasuo Ohnishi, Koji Ichinose
Type II polyketide synthase (PKS) iteratively generates a nascent polyketide thioester of acyl carrier protein (ACP), which is structurally modified to produce an ACP-free intermediate towards its final metabolite. However, the timing of ACP off-loading is not well defined due to the lack of an apparent thioesterase (TE) among relevant biosynthetic enzymes. Here, ActIV, which had been assigned as a second ring cyclase (CYC) in actinorhodin (ACT) biosynthesis, was shown to possess a TE activity in vitro with a model substrate, anthraquinone-2-carboxylic acid-N-acetylcysteamine...
November 29, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27875313/direct-involvement-of-the-master-nitrogen-metabolism-regulator-glnr-in-antibiotic-biosynthesis-in-streptomyces
#4
Jun-Mei He, Hong Zhu, Guo-Song Zheng, Pan-Pan Liu, Jin Wang, Guo-Ping Zhao, Guo-Qiang Zhu, Wei-Hong Jiang, Yin-Hua Lu
GlnR, an OmpR-like orphan two-component system response regulator, is a master regulator of nitrogen metabolism in the genus Streptomyces. In this work, evidence that GlnR is also directly involved in the regulation of antibiotic biosynthesis is provided. In the model strain Streptomyces coelicolor M145, an in-frame deletion of glnR resulted in markedly increased actinorhodin (ACT) production but reduced undecylprodigiosin (RED) biosynthesis when exposed to R2YE culture medium. Transcriptional analysis coupled with DNA-binding studies revealed that GlnR represses ACT but activates RED production directly via the pathway-specific activator genes actII-ORF4 and redZ, respectively...
November 8, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27822533/osdr-of-streptomyces-coelicolor-and-the-dormancy-regulator-devr-of-mycobacterium-tuberculosis-control-overlapping-regulons
#5
Mia Urem, Teunke van Rossum, Giselda Bucca, Geri F Moolenaar, Emma Laing, Magda A Świątek-Połatyńska, Joost Willemse, Elodie Tenconi, Sébastien Rigali, Nora Goosen, Colin P Smith, Gilles P van Wezel
Two-component regulatory systems allow bacteria to respond adequately to changes in their environment. In response to a given stimulus, a sensory kinase activates its cognate response regulator via reversible phosphorylation. The response regulator DevR activates a state of dormancy under hypoxia in Mycobacterium tuberculosis, allowing this pathogen to escape the host defense system. Here, we show that OsdR (SCO0204) of the soil bacterium Streptomyces coelicolor is a functional orthologue of DevR. OsdR, when activated by the sensory kinase OsdK (SCO0203), binds upstream of the DevR-controlled dormancy genes devR, hspX, and Rv3134c of M...
May 2016: MSystems
https://www.readbyqxmd.com/read/27797866/a-role-of-nitrite-reductase-nirbd-for-no-homeostatic-regulation-in-streptomyces-coelicolor-a3-2
#6
Yuriya Yukioka, Tsukiko Tanahashi, Keisuke Shida, Haruka Oguchi, Shota Ogawa, Chiaki Saito, Shunsuke Yajima, Shinsaku Ito, Kanju Ohsawa, Hirofumi Shoun, Yasuyuki Sasaki
Although nitric oxide (NO) is an important signaling molecule in bacteria and higher organisms, excessive intracellular NO is highly reactive and dangerous. Therefore, living cells need strict regulation systems for cellular NO homeostasis. Recently, we discovered that Streptomyces coelicolor A3(2) retains the nitrogen oxide cycle (NO3(-)→NO2(-)→NO→NO3(-)) and nitrite removal system. The nitrogen oxide cycle regulates cellular NO levels, thereby controlling secondary metabolism initiation (red-pigmented antibiotic, RED production) and morphological differentiation...
October 18, 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27759388/how-to-make-a-glycopeptide-a-synthetic-biology-approach-to-expand-antibiotic-chemical-diversity
#7
Grace Yim, Wenliang Wang, Maulik N Thaker, Stephanie Tan, Gerard D Wright
Modification of natural product backbones is a proven strategy for the development of clinically useful antibiotics. Such modifications have traditionally been achieved through medicinal chemistry strategies or via in vitro enzymatic activities. In an orthogonal approach, engineering of biosynthetic pathways using synthetic biology techniques can generate chemical diversity. Here we report the use of a minimal teicoplanin class glycopeptide antibiotic (GPA) scaffold expressed in a production-optimized Streptomyces coelicolor strain to expand GPA chemical diversity...
September 9, 2016: ACS Infectious Diseases
https://www.readbyqxmd.com/read/27731336/characterization-of-the-biosynthetic-gene-cluster-ata-for-the-a201a-aminonucleoside-antibiotic-from-saccharothrix-mutabilis-subsp-capreolus
#8
Irene Saugar, Brian Molloy, Eloisa Sanz, María Blanca Sánchez, María Fernández-Lobato, Antonio Jiménez
Antibiotic A201A produced by Saccharothrix mutabilis subsp. capreolus NRRL3817 contains an aminonucleoside (N(6), N(6)-dimethyl-3'-amino-3'-deoxyadenosyl), a polyketide (α-methyl-p-coumaric acid) and a disaccharide moiety. The heterologous expression in Streptomyces lividans and Streptomyces coelicolor of a S. mutabilis genomic region of ~34 kb results in the production of A201A, which was identified by microbiological, biochemical and physicochemical approaches, and indicating that this region may contain the entire A201A biosynthetic gene cluster (ata)...
October 12, 2016: Journal of Antibiotics
https://www.readbyqxmd.com/read/27729505/peptidoglycan-recycling-in-gram-positive-bacteria-is-crucial-for-survival-in-stationary-phase
#9
Marina Borisova, Rosmarie Gaupp, Amanda Duckworth, Alexander Schneider, Désirée Dalügge, Maraike Mühleck, Denise Deubel, Sandra Unsleber, Wenqi Yu, Günther Muth, Markus Bischoff, Friedrich Götz, Christoph Mayer
: Peptidoglycan recycling is a metabolic process by which Gram-negative bacteria reutilize up to half of their cell wall within one generation during vegetative growth. Whether peptidoglycan recycling also occurs in Gram-positive bacteria has so far remained unclear. We show here that three Gram-positive model organisms, Staphylococcus aureus, Bacillus subtilis, and Streptomyces coelicolor, all recycle the sugar N-acetylmuramic acid (MurNAc) of their peptidoglycan during growth in rich medium...
October 11, 2016: MBio
https://www.readbyqxmd.com/read/27718094/dasr-positively-controls-monensin-production-at-two-level-regulation-in-streptomyces-cinnamonensis
#10
Yue Zhang, Chun-Yan Lin, Xiao-Mei Li, Zheng-Kun Tang, Jianjun Qiao, Guang-Rong Zhao
The polyether ionophore antibiotic monensin is produced by Streptomyces cinnamonensis and is used as a coccidiostat for chickens and growth-promoting agent for cattle. Monensin biosynthetic gene cluster has been cloned and partially characterized. The GntR-family transcription factor DasR regulates antibiotic production and morphological development in Streptomyces coelicolor and Saccharopolyspora erythraea. In this study, we identified and characterized the two-level regulatory cascade of DasR to monensin production in S...
October 8, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27687228/rraas2-requires-both-scaffold-domains-of-rnase-es-for-high-affinity-binding-and-inhibitory-action-on-the-ribonucleolytic-activity
#11
Jihune Heo, Daeyoung Kim, Minju Joo, Boeun Lee, Sojin Seo, Jaejin Lee, Saemee Song, Ji-Hyun Yeom, Nam-Chul Ha, Kangseok Lee
RraA is a protein inhibitor of RNase E (Rne), which catalyzes the endoribonucleolytic cleavage of a large proportion of RNAs in Escherichia coli. The antibiotic-producing bacterium Streptomyces coelicolor also contains homologs of RNase E and RraA, designated as RNase ES (Rns), RraAS1, and RraAS2, respectively. Here, we report that RraAS2 requires both scaffold domains of RNase ES for high-affinity binding and inhibitory action on the ribonucleolytic activity. Analyses of the steady-state level of RNase E substrates indicated that coexpression of RraAS2 in E...
October 2016: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/27680538/engineering-of-a-microbial-coculture-of-escherichia-coli-strains-for-the-biosynthesis-of-resveratrol
#12
José M Camacho-Zaragoza, Georgina Hernández-Chávez, Fabian Moreno-Avitia, René Ramírez-Iñiguez, Alfredo Martínez, Francisco Bolívar, Guillermo Gosset
BACKGROUND: Resveratrol is a plant natural product with many health-protecting effects which makes it an attractive chemical both for academic studies and industrial purposes. However, the low quantities naturally produced by plants as well as the unsustainable procedures of extraction, purification and concentration have prompted many biotechnological approaches to produce this chemical in large quantities from renewable sources. None of these approaches have considered a microbial coculture strategy to produce this compound...
September 29, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27669158/trpm-a-small-protein-modulating-tryptophan-biosynthesis-and-morpho-physiological-differentiation-in-streptomyces-coelicolor-a3-2
#13
Emilia Palazzotto, Giuseppe Gallo, Giovanni Renzone, Anna Giardina, Alberto Sutera, Joohee Silva, Celinè Vocat, Luigi Botta, Andrea Scaloni, Anna Maria Puglia
In the model actinomycete Streptomyces coelicolor A3(2), small open reading frames encoding proteins with unknown functions were identified in several amino acid biosynthetic gene operons, such as SCO2038 (trpX) in the tryptophan trpCXBA locus. In this study, the role of the corresponding protein in tryptophan biosynthesis was investigated by combining phenotypic and molecular analyses. The 2038KO mutant strain was characterized by delayed growth, smaller aerial hyphae and reduced production of spores and actinorhodin antibiotic, with respect to the WT strain...
2016: PloS One
https://www.readbyqxmd.com/read/27637875/glnr-and-phop-directly-regulate-the-transcription-of-genes-encoding-starch-degrading-amylolytic-enzymes-in-saccharopolyspora-erythraea
#14
Ya Xu, Cheng-Heng Liao, Li-Li Yao, Xu Ye, Bang-Ce Ye
Starch-degrading enzymes hydrolyze starch- and starch-derived oligosaccharides to yield glucose. We investigated the transcriptional regulation of genes encoding starch-degrading enzymes in the industrial actinobacteria Saccharopolyspora erythraea We observed that most genes encoding amylolytic enzymes (one α-amylase, one glucoamylase and four α-glucosidases) were regulated by GlnR and PhoP, which are global regulators of nitrogen and phosphate metabolism, respectively. Electrophoretic mobility shift assays and RT-PCR analyses demonstrated that GlnR and PhoP directly interact with their promoter regions, and collaboratively or competitively activate their transcription...
September 16, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27634042/disruption-of-macrodomain-protein-sco6735-increases-antibiotic-production-in-streptomyces-coelicolor
#15
Jasna Lalić, Melanija Posavec Marjanović, Luca Palazzo, Dragutin Perina, Igor Sabljić, Roko Žaja, Thomas Colby, Bruna Pleše, Mirna Halasz, Gytis Jankevicius, Giselda Bucca, Marijan Ahel, Ivan Matić, Helena Ćetković, Marija Luić, Andreja Mikoč, Ivan Ahel
ADP-ribosylation is a post-translational modification that can alter the physical and chemical properties of target proteins and controls many important cellular processes. Macrodomains are evolutionarily conserved structural domains that bind ADP-ribose derivatives and are found in proteins with diverse cellular functions. Some proteins from the macrodomain family can hydrolyze ADP-ribosylated substrates and therefore reverse this post-translational modification. Bacteria and Streptomyces, in particular, are known to utilize protein ADP-ribosylation, yet very little is known about their enzymes that synthesise and remove this modification...
September 15, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27605472/characterization-of-a-putative-nsrr-homologue-in-streptomyces-venezuelae-reveals-a-new-member-of-the-rrf2-superfamily
#16
John T Munnoch, Ma Teresa Pellicer Martinez, Dimitri A Svistunenko, Jason C Crack, Nick E Le Brun, Matthew I Hutchings
Members of the Rrf2 superfamily of transcription factors are widespread in bacteria but their functions are largely unexplored. The few that have been characterized in detail sense nitric oxide (NsrR), iron limitation (RirA), cysteine availability (CymR) and the iron sulfur (Fe-S) cluster status of the cell (IscR). In this study we combined ChIP- and dRNA-seq with in vitro biochemistry to characterize a putative NsrR homologue in Streptomyces venezuelae. ChIP-seq analysis revealed that rather than regulating the nitrosative stress response like Streptomyces coelicolor NsrR, Sven6563 binds to a conserved motif at a different, much larger set of genes with a diverse range of functions, including a number of regulators, genes required for glutamine synthesis, NADH/NAD(P)H metabolism, as well as general DNA/RNA and amino acid/protein turn over...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27605107/bereta-a-systematic-method-for-identifying-target-transcriptional-regulators-to-enhance-microbial-production-of-chemicals
#17
Minsuk Kim, Gwanggyu Sun, Dong-Yup Lee, Byung-Gee Kim
MOTIVATION: Modulation of regulatory circuits governing the metabolic processes is a crucial step for developing microbial cell factories. Despite the prevalence of in silico strain design algorithms, most of them are not capable of predicting required modifications in regulatory networks. Although a few algorithms may predict relevant targets for transcriptional regulator (TR) manipulations, they have limited reliability and applicability due to their high dependency on the availability of integrated metabolic/regulatory models...
September 7, 2016: Bioinformatics
https://www.readbyqxmd.com/read/27605017/discovery-of-unusual-biaryl-polyketides-by-activation-of-a-silent-streptomyces-venezuelae-biosynthetic-gene-cluster
#18
Anyarat Thanapipatsiri, Juan Pablo Gomez-Escribano, Lijiang Song, Maureen J Bibb, Mahmoud Al-Bassam, Govind Chandra, Arinthip Thamchaipenet, Gregory L Challis, Mervyn J Bibb
Comparative transcriptional profiling of a ΔbldM mutant of Streptomyces venezuelae with its unmodified progenitor revealed that the expression of a cryptic biosynthetic gene cluster containing both type I and type III polyketide synthase genes is activated in the mutant. The 29.5 kb gene cluster, which was predicted to encode an unusual biaryl metabolite, which we named venemycin, and potentially halogenated derivatives, contains 16 genes including one-vemR-that encodes a transcriptional activator of the large ATP-binding LuxR-like (LAL) family...
November 17, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27604626/deletion-of-the-hypothetical-protein-sco2127-of-streptomyces-coelicolor-allowed-identification-of-a-new-regulator-of-actinorhodin-production
#19
Tierrafría Víctor H, Licona-Cassani Cuauhtemoc, Maldonado-Carmona Nidia, Romero-Rodríguez Alba, Centeno-Leija Sara, Marcellin Esteban, Rodríguez-Sanoja Romina, Beatriz Ruiz-Villafán, Nielsen Lars K, Sánchez Sergio
Although the specific function of SCO2127 remains elusive, it has been assumed that this hypothetical protein plays an important role in carbon catabolite regulation and therefore in antibiotic biosynthesis in Streptomyces coelicolor. To shed light on the functional relationship of SCO2127 to the biosynthesis of actinorhodin, a detailed analysis of the proteins differentially produced between the strain M145 and the Δsco2127 mutant of S. coelicolor was performed. The delayed morphological differentiation and impaired production of actinorhodin showed by the deletion strain were accompanied by increased abundance of gluconeogenic enzymes, as well as downregulation of both glycolysis and acetyl-CoA carboxylase...
November 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27572512/molecular-characterization-of-sco0765-as-a-cellotriose-releasing-endo-%C3%AE-1-4-cellulase-from-streptomyces-coelicolor-a-3
#20
Joo-Bin Hong, Vijayalakshmi Dhakshnamoorthy, Chang-Ro Lee
The sco0765 gene was annotated as a glycosyl hydrolase family 5 endoglucanase from the genomic sequence of Streptomyces coelicolor A3(2) and consisted of 2,241 bp encoding a polypeptide of 747 amino acids (molecular weight of 80.5 kDa) with a 29-amino acid signal peptide for secretion. The SCO0765 recombinant protein was heterogeneously over-expressed in Streptomyces lividans TK24 under the control of a strong ermE* promoter. The purified SCO0765 protein showed the expected molecular weight of the mature form (718 aa, 77...
September 2016: Journal of Microbiology / the Microbiological Society of Korea
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