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Streptomyces coelicolor

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https://www.readbyqxmd.com/read/29784883/cytochrome-bd-oxidase-has-an-important-role-in-sustaining-growth-and-development-of-streptomyces-coelicolor-a3-2-under-oxygen-limiting-conditions
#1
Marco Fischer, Dörte Falke, Carolin Naujoks, R Gary Sawers
Streptomyces coelicolor A3 (2) is a filamentously growing, spore-forming, obligately aerobic actinobacterium that uses both a copper- aa3 - type cytochrome c oxidase and a cytochrome bd oxidase to respire oxygen. Using defined knock-out mutants we demonstrated that either of these terminal oxidases was capable of allowing the bacterium to grow and complete its developmental cycle. The genes encoding the bcc complex and the aa3 oxidase are clustered at a single locus. Using western blot analyses we showed that the bcc-aa3 oxidase branch is more prevalent than the bd oxidase in spores...
May 21, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29784711/quantitative-proteome-and-phosphoproteome-analyses-of-streptomyces-coelicolor-reveal-proteins-and-phosphoproteins-modulating-differentiation-and-secondary-metabolism
#2
Beatriz Rioseras, Pavel V Sliaha, Vladimir Gorshkov, Paula Yagüe, Maria T López-García, Nathaly González-Quiñónez, Sergey Kovalchuk, Adelina Rogowska-Wrzesinska, Ole N Jensen, Angel Manteca
Streptomycetes are multicellular bacteria with complex developmental cycles. They are of biotechnological importance as they produce most bioactive compounds used in biomedicine, e.g. antibiotic, antitumoral and immunosupressor compounds. Streptomyces genomes encode a large number of Ser/Thr/Tyr kinases, making this genus an outstanding model for the study of bacterial protein phosphorylation events. We used mass spectrometry based quantitative proteomics and phosphoproteomics to characterize bacterial differentiation and activation of secondary metabolism of Streptomyces coelicolor...
May 21, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29753326/biosynthesis-of-angelyl-coa-in-saccharomyces-cerevisiae
#3
Roberta Callari, David Fischer, Harald Heider, Nora Weber
BACKGROUND: The angelic acid moiety represents an essential modification in many biologically active products. These products are commonly known as angelates and several studies have demonstrated their therapeutic benefits, including anti-inflammatory and anti-cancer effects. However, their availability for use in the development of therapeutics is limited due to poor extraction yields. Chemical synthesis has been achieved but its complexity prevents application, therefore microbial production may offer a promising alternative...
May 12, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29746664/translational-control-plays-an-important-role-in-the-adaptive-heat-shock-response-of-streptomyces-coelicolor
#4
Giselda Bucca, Radhika Pothi, Andrew Hesketh, Carla Möller-Levet, David A Hodgson, Emma E Laing, Graham R Stewart, Colin P Smith
Stress-induced adaptations require multiple levels of regulation in all organisms to repair cellular damage. In the present study we evaluated the genome-wide transcriptional and translational changes following heat stress exposure in the soil-dwelling model actinomycete bacterium, Streptomyces coelicolor. The combined analysis revealed an unprecedented level of translational control of gene expression, deduced through polysome profiling, in addition to transcriptional changes. Our data show little correlation between the transcriptome and 'translatome'; while an obvious downward trend in genome wide transcription was observed, polysome associated transcripts following heat-shock showed an opposite upward trend...
May 9, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29743540/sporulation-specific-cell-division-defects-in-ylme-mutants-of-streptomyces-coelicolor-are-rescued-by-additional-deletion-of-ylmd
#5
Le Zhang, Joost Willemse, Paul A Hoskisson, Gilles P van Wezel
Cell division during the reproductive phase of the Streptomyces life-cycle requires tight coordination between synchronous formation of multiple septa and DNA segregation. One remarkable difference with most other bacterial systems is that cell division in Streptomyces is positively controlled by the recruitment of FtsZ by SsgB. Here we show that deletion of ylmD (SCO2081) or ylmE (SCO2080), which lie in operon with ftsZ in the dcw cluster of actinomycetes, has major consequences for sporulation-specific cell division in Streptomyces coelicolor...
May 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29730661/improvement-of-spinosad-production-upon-utilization-of-oils-and-manipulation-of-%C3%AE-oxidation-in-a-high-producing-saccharopolyspora-spinosa-strain
#6
Ying Huang, Xiaolin Zhang, Chen Zhao, Xuhui Zhuang, Lin Zhu, Chao Guo, Yuan Song
Spinosad, a member of polyketide-derived macrolides produced in the actinomycete Saccharopolyspora spinosa, has been developed as a broad-spectrum and effective insecticide. The β-oxidation pathway could be an important source of building blocks for the biosynthesis of spinosad, thus the effect of vegetable oils on the production of spinosad in a high-yield strain was investigated. The spinosad production increased significantly with the addition of strawberry seed oil (511.64 mg/L) and camellia oil (520.07 mg/L) compared to the control group without oil (285...
May 4, 2018: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29719789/biosynthesis-of-antibiotic-chuangxinmycin-from-actinoplanes-tsinanensis
#7
Yuanyuan Shi, Zhibo Jiang, Xingxing Li, Lijie Zuo, Xuan Lei, Liyan Yu, Linzhuan Wu, Jiandong Jiang, Bin Hong
Chuangxinmycin is an antibiotic isolated from Actinoplanes tsinanensis CPCC 200056 in the 1970s with a novel indole-dihydrothiopyran heterocyclic skeleton. Chuangxinmycin showed in vitro antibacterial activity and in vivo efficacy in mouse infection models as well as preliminary clinical trials. But the biosynthetic pathway of chuangxinmycin has been obscure since its discovery. Herein, we report the identification of a stretch of DNA from the genome of A. tsinanensis CPCC 200056 that encodes genes for biosynthesis of chuangxinmycin by bioinformatics analysis...
March 2018: Acta Pharmaceutica Sinica. B
https://www.readbyqxmd.com/read/29693566/specificity-of-induction-of-glycopeptide-antibiotic-resistance-in-the-producing-actinomycetes
#8
Elisa Binda, Pamela Cappelletti, Flavia Marinelli, Giorgia Letizia Marcone
Glycopeptide antibiotics are drugs of last resort for treating severe infections caused by Gram-positive pathogens. It is widely believed that glycopeptide-resistance determinants ( van genes) are ultimately derived from the producing actinomycetes. We hereby investigated the relationship between the antimicrobial activity of vancomycin and teicoplanins and their differential ability to induce van gene expression in Actinoplanes teichomyceticus —the producer of teicoplanin—and Nonomuraea gerenzanensis —the producer of the teicoplanin-like A40926...
April 25, 2018: Antibiotics
https://www.readbyqxmd.com/read/29685972/desferrioxamine-biosynthesis-diverse-hydroxamate-assembly-by-substrate-tolerant-acyl-transferase-desc
#9
Jade L Ronan, Nadia Kadi, Stephen A McMahon, James H Naismith, Lona M Alkhalaf, Gregory L Challis
Hydroxamate groups play key roles in the biological function of diverse natural products. Important examples include trichostatin A, which inhibits histone deacetylases via coordination of the active site zinc(II) ion with a hydroxamate group, and the desferrioxamines, which use three hydroxamate groups to chelate ferric iron. Desferrioxamine biosynthesis in Streptomyces species involves the DesD-catalysed condensation of various N -acylated derivatives of N -hydroxycadaverine with two molecules of N -succinyl- N -hydroxycadaverine to form a range of linear and macrocyclic tris-hydroxamates...
June 5, 2018: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/29679043/identification-and-heterologous-expression-of-the-actinoallolide-biosynthetic-gene-cluster
#10
Yuki Inahashi, Taro Shiraishi, Akira Také, Atsuko Matsumoto, Yōko Takahashi, Satoshi Ōmura, Tomohisa Kuzuyama, Takuji Nakashima
Actinoallolides are anti-trypanosomal macrolides isolated from the secondary metabolites of two endophytic actinomycete strains, Actinoallomurus fulvus MK10-036 and K09-0307. A putative actinoallolide biosynthetic gene cluster was predicted from the genome sequence of the strain K09-0307. The gene cluster spans a contiguous 53 kb DNA region that comprises seven genes encoding three PKSs (aalA1, aalA2, and aalA3), cytochrome P450 (aalB), acyl-CoA dehydrogenase (aalC), crotonyl-CoA reductase (aalD), and TetR family regulator (aalR)...
April 20, 2018: Journal of Antibiotics
https://www.readbyqxmd.com/read/29677594/mechanisms-of-oxidative-stress-caused-by-cuo-nanoparticles-to-membranes-of-the-bacterium-streptomyces-coelicolor-m145
#11
Xiaomei Liu, Jingchun Tang, Lan Wang, John P Giesy
Toxic effects of widely used CuO nanoparticles (NPs) on the genus Streptomyces has been seldom studied. This work investigated toxicities of several sizes of CuO nanoparticles (NPs) to Streptomyces coelicolor M145 (S. coelicolor M145). Compared with NPs, toxicity of micrometer-sized CuO on M145 was trivial. In 0.9% NaCl, when the concentration of CuO NPs was 100 mg/L, survival of bacteria increased from 18.3% in 20 nm particles to 31.1% in 100 nm particles. With increasing concentrations of CuO, the level of ROS gradually increased and there were significant differences (p < 0...
April 17, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29650021/overexpression-of-a-type-iii-pks-gene-affording-novel-violapyrones-with-enhanced-anti-influenza-a-virus-activity
#12
Lukuan Hou, Huiming Huang, Huayue Li, Shuyao Wang, Jianhua Ju, Wenli Li
BACKGROUND: Type III polyketide synthases (PKSs) are simple homodimer ketosynthases that distribute across plants, fungi, and bacteria, catalyzing formation of pyrone- and resorcinol-types aromatic polyketides with various bioactivities. The broad substrate promiscuity displayed by type III PKSs makes them wonderful candidates for expanding chemical diversity of polyketides. RESULTS: Violapyrone B (VLP B, 10), an α-pyrone compound produced by deepsea-derived Streptomyces somaliensis SCSIO ZH66, is encoded by a type III PKS VioA...
April 12, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29629858/pseudomonas-aeruginosa-puma-acts-on-an-endogenous-phenazine-to-promote-self-resistance
#13
Abigail J Sporer, Christopher Beierschmitt, Anastasia Bendebury, Katherine E Zink, Alexa Price-Whelan, Marisa C Buzzeo, Laura M Sanchez, Lars E P Dietrich
The activities of critical metabolic and regulatory proteins can be altered by exposure to natural or synthetic redox-cycling compounds. Many bacteria, therefore, possess mechanisms to transport or transform these small molecules. The opportunistic pathogen Pseudomonas aeruginosa PA14 synthesizes phenazines, redox-active antibiotics that are toxic to other organisms but have beneficial effects for their producer. Phenazines activate the redox-sensing transcription factor SoxR and thereby induce the transcription of a small regulon, including the operon mexGHI-opmD, which encodes an efflux pump that transports phenazines, and PA14_35160 (pumA), which encodes a putative monooxygenase...
April 9, 2018: Microbiology
https://www.readbyqxmd.com/read/29619920/erratum-streptomyces-coelicolor-strains-lacking-polyprenol-phosphate-mannose-synthase-and-protein-o-mannosyl-transferase-are-hyper-susceptible-to-multiple-antibiotics
#14
Robert Howlett, Nicholas Read, Anpu Varghese, Charles Kershaw, Y Hancock, Margaret C M Smith
No abstract text is available yet for this article.
April 2018: Microbiology
https://www.readbyqxmd.com/read/29596827/structural-and-biochemical-characterization-of-the-type-ii-log-protein-from-streptomyces-coelicolor-a3
#15
Hogyun Seo, Kyung-Jin Kim
Streptomyces coelicolor A3 contains Sc5140, a gene coding for poorly understood bacterial LOG-like protein. In this study, we determined the crystal structure of Sc5140 and found it resembles the overall structure of other type-II LOGs. In addition, Sc5140 exhibited phosphoribohydrolase activity against adenosine monophosphate (AMP), indicating that it had the same function as known type-II LOGs. Based on these results, we designated Sc5140 as ScLOGII. We performed docking calculations of AMP into the ScLOGII structure, which suggested the mode of binding for type-II LOG with their AMP substrate...
March 26, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29583114/glnr-and-phop-regulate-%C3%AE-glucosidases-involved-in-cellulose-digestion-in-response-to-nitrogen-and-phosphate-availability
#16
Ya Xu, Bang-Ce Ye
The limited catalytic efficiency of cellulose-degrading enzymes restricts cellulose digestion. We investigated the transcriptional regulation of genes encoding key cellulose degrading enzymes, namely β-glucosidases, in the industrial actinobacterium Saccharopolyspora erythraea. We observed that the expression of most β-glucosidase-encoding genes was controlled by the availability of nitrogen and phosphate via their respective global regulators, namely GlnR and PhoP. Electrophoretic mobility shift assay demonstrated that GlnR and PhoP bound directly to the promoters of β-glucosidase-encoding genes...
March 27, 2018: Microbiology
https://www.readbyqxmd.com/read/29545785/argr-of-streptomyces-coelicolor-is-a-pleiotropic-transcriptional-regulator-effect-on-the-transcriptome-antibiotic-production-and-differentiation-in-liquid-cultures
#17
Alma Botas, Rosario Pérez-Redondo, Antonio Rodríguez-García, Rubén Álvarez-Álvarez, Paula Yagüe, Angel Manteca, Paloma Liras
ArgR is a well-characterized transcriptional repressor controlling the expression of arginine and pyrimidine biosynthetic genes in bacteria. In this work, the biological role of Streptomyces coelicolor ArgR was analyzed by comparing the transcriptomes of S. coelicolor Δ argR and its parental strain, S. coelicolor M145, at five different times over a 66-h period. The effect of S. coelicolor ArgR was more widespread than that of the orthologous protein of Escherichia coli , affecting the expression of 1544 genes along the microarray time series...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29544487/comprehensive-subcellular-topologies-of-polypeptides-in-streptomyces
#18
Konstantinos C Tsolis, Evridiki-Pandora Tsare, Georgia Orfanoudaki, Tobias Busche, Katerina Kanaki, Reshmi Ramakrishnan, Frederic Rousseau, Joost Schymkowitz, Christian Rückert, Jörn Kalinowski, Jozef Anné, Spyridoula Karamanou, Maria I Klapa, Anastassios Economou
BACKGROUND: Members of the genus Streptomyces are Gram-positive bacteria that are used as important cell factories to produce secondary metabolites and secrete heterologous proteins. They possess some of the largest bacterial genomes and thus proteomes. Understanding their complex proteomes and metabolic regulation will improve any genetic engineering approach. RESULTS: Here, we performed a comprehensive annotation of the subcellular localization of the proteome of Streptomyces lividans TK24 and developed the Subcellular Topology of Polypeptides in Streptomyces database (SToPSdb) to make this information widely accessible...
March 15, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29515563/the-sco4117-ecf-sigma-factor-pleiotropically-controls-secondary-metabolism-and-morphogenesis-in-streptomyces-coelicolor
#19
María T López-García, Paula Yagüe, Nathaly González-Quiñónez, Beatriz Rioseras, Angel Manteca
Extracytoplasmic function (ECF) sigma factors are a major type of bacterial signal-transducers whose biological functions remain poorly characterized in streptomycetes. In this work we studied SCO4117, a conserved ECF sigma factor from the ECF52 family overexpressed during substrate and aerial mycelium stages. The ECF52 sigma factors harbor, in addition to the ECF sigma factor domain, a zinc finger domain, a transmembrane region, a proline-rich C-terminal extension, and a carbohydrate-binding domain. This class of ECF sigma factors is exclusive to Actinobacteria...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29515546/insight-into-the-molecular-mechanism-of-the-transcriptional-regulation-of-amtb-operon-in-streptomyces-coelicolor
#20
Zhendong Li, Xinqiang Liu, Jingzhi Wang, Ying Wang, Guosong Zheng, Yinhua Lu, Guoping Zhao, Jin Wang
In Streptomyces coelicolor , amtB transcription is promptly regulated by the global nitrogen regulator GlnR. Although the GlnR binding cis -element has been characterized in amtB promoter, consisting of three GlnR boxes of a3-b3 , a1-b1 , and a2-b2 , its role in GlnR-mediated transcriptional regulation remains unclear. Here, we showed that GlnR had different binding affinity against each pair of GlnR binding sites in amtB promoter (i.e., a3-b3 , a1-b1 , and a2-b2 sites), and GlnR was able to bind a3-b3 and a1-b1 , respectively, but not a2-b2 alone...
2018: Frontiers in Microbiology
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