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Apoptosis in cancer cells

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https://www.readbyqxmd.com/read/28927154/resistance-to-the-mtor-inhibitor-everolimus-is-reversed-by-the-downregulation-of-survivin-in-breast-cancer-cells
#1
Ludovica Taglieri, Francesca De Iuliis, Anna Giuffrida, Sabrina Giantulli, Ida Silvestri, Susanna Scarpa
Everolimus (RAD001) is an inhibitor of mammalian target of rapamycin used in combination with exemestane to treat hormone receptor-positive advanced breast cancer. However, not all patients are equally sensitive to RAD001 and certain patients develop resistance. Therefore, the present study analyzed the mechanisms involved in the resistance of breast cancer cells to RAD001 in order to identify a potential tool to overcome it. The effects of RAD001 on the inhibition of cell viability, on the induction of apoptosis and autophagy and on the regulation of survivin, an anti-apoptotic protein, were evaluated in two breast cancer cell lines: BT474 (luminal B) and MCF7 (luminal A)...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927147/long-term-changes-in-the-properties-of-skin-derived-fibroblasts-following-irradiation-of-the-head-and-neck
#2
Thomas Gehrke, Agmal Scherzad, Stephan Hackenberg, Pascal Ickrath, Philipp Schendzielorz, Rudolf Hagen, Norbert Kleinsasser
The tumor stroma performs an important role in carcinogenesis. It predominantly consists of fibroblasts and the connective tissue produced by them, and undergoes a multitude of interactions with the surrounding cancer cells. Since irradiation is part of the majority of therapeutic strategies for head and neck squamous cell carcinoma, more information regarding the effects of a previous irradiation on the tumor stroma is desirable. In the present study, fibroblasts were cultivated from human non-irradiated and pre-irradiated skin of the neck for 48 h...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927146/impacts-of-survivin-and-caspase-3-on-apoptosis-and-angiogenesis-in-oral-cancer
#3
Shuxia Li, Yanqi Yang, Yanping Ding, Xiaofei Tang, Zheng Sun
The present study aimed to investigate the impact of survivin and caspase-3 on apoptosis and angiogenesis in oral cancer. A total of 16 oral leukoplakia cases accompanied by low-moderate epithelial dysplasia, 12 cases of oral leukoplakia accompanied by severe epithelial dysplasia, 17 cases of high-moderate differentiated oral squamous cell carcinoma and 10 cases of normal oral mucosa were selected. Immunohistochemistry was used to detect the expression levels of survivin, caspase-3, and caspase inhibitor factor VIII in lesions from each group...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927142/tumour-growth-suppressive-effect-of-arsenic-trioxide-in-squamous-cell-lung-carcinoma
#4
Leanne Lee Leung, Sze-Kwan Lam, Yuan-Yuan Li, James Chung-Man Ho
Lung squamous cell carcinoma (SCC) is the second most common subtype of non-small cell lung carcinoma. The anticancer effects of arsenic trioxide (ATO) in lung adenocarcinoma and small-cell lung cancer have previously been reported; however its effects in SCC remain unclear. An MTT assay and western blot analysis were performed to determine cell viability and protein expression, respectively, in the SK-MES-1 and SW900 SCC cell lines following treatment with ATO. Phosphatidylserine externalization, mitochondrial membrane depolarization and cell cycle distribution were studied using flow cytometry and the in vivo effects of ATO on tumour growth were investigated with a xenograft model...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927141/catalpol-promotes-cellular-apoptosis-in-human-hct116-colorectal-cancer-cells-via-microrna-200-and-the-downregulation-of-pi3k-akt-signaling-pathway
#5
Lan Liu, Hongwei Gao, Hongbo Wang, Yuan Zhang, Weihua Xu, Sen Lin, Hongjuan Wang, Qiong Wu, Jianqiang Guo
Catalpol is an effective active ingredient that functions as a diuretic and laxative, and exhibits blood sugar-lowering, liver protective, anti-aging and anticancer effects. In traditional Chinese medicine, catalpol is believed to be Yin nourishing. The anticancer effect of catalpol on human HCT116 colorectal cancer cells were investigated and the mechanism of action was evaluated. Cellular viability was detected using an MTT assay. Caspase-3 and caspase-9 activity, cellular apoptosis and nucleic morphology were analyzed using caspase-3 and caspase-9 activity assay kits, flow cytometric assays and DAPI staining assay, respectively...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927132/downregulation-of-esophageal-cancer-related-gene-4-promotes-proliferation-and-migration-of-hepatocellular-carcinoma
#6
Shujian Ge, Yali Xu, Hongliang Wang, Yaxin Sun, Xiangguo Tian, Zhixin Cao, Xiaoyan Lin, Jiawen Xu, Qiangxiu Wang
Esophageal cancer-related gene 4 (ECRG4) is a candidate tumor suppressor gene, which is involved in cell apoptosis, migration, infection and inflammation responsiveness; however, its expression level and clinical significance in hepatocellular carcinoma (HCC) remains unclear. In the present study, the authors aim to evaluate the clinical significance and potential role of ECRG4 in HCC. Level of ECRG4 protein expression in HCC and peripheral tissues was investigated in tissue specimens obtained from 56 consecutive HCC patients by immunohistochemistry...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927122/mechanism-of-egcg-promoting-apoptosis-of-mcf-7-cell-line-in-human-breast-cancer
#7
Chao-You Huang, Zheng Han, Xi Li, Hui-Hua Xie, Shan-Shan Zhu
The aim of the present study was to investigate the effects of epigallocatechin-3-gallate (EGCG) on the apoptosis of the human MCF-7 cancer cell line and the underlying mechanism. MCF-7 cells were divided into the control group and EGCG groups. The proliferation of MCF-7 cells in the two groups was determined using MTT and apoptosis was examined using flow cytometry. Western blot analysis and qRT-PCR were used to analyze P53 and Bcl-2 expression levels. The silencing effect of specific siRNA was evaluated using RT-PCR and western blot analysis...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927115/wee1-inhibition-by-mk1775-as-a-single-agent-therapy-inhibits-ovarian-cancer-viability
#8
Minghui Zhang, Donye Dominguez, Siqi Chen, Jie Fan, Lei Qin, Alan Long, Xia Li, Yi Zhang, Huirong Shi, Bin Zhang
Wee1-like protein kinase (WEE1) physiologically serves a key function in maintaining the integrity of the cell genome through mediating the activation of cyclin-dependent kinase (CDK)1 and CDK2. Increased expression of WEE1 has been associated with the poor prognosis of patients with ovarian cancer. The present study aimed at examining the in vitro and in vivo antitumor activity of MK1775, a potent pharmacological inhibitor of WEE1, as a single agent against ovarian cancer cells. The cytotoxicity of MK1775 was examined in a panel of tumor cells using MTT in vitro...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927114/microrna-383-5p-acts-as-a-prognostic-marker-and-inhibitor-of-cell-proliferation-in-lung-adenocarcinoma-by-cancerous-inhibitor-of-protein-phosphatase-2a
#9
Shasha Zhao, Xinyuan Gao, Shuzhi Zang, Yunxia Li, Xianjun Feng, Xiaomei Yuan
Lung cancer is the leading cause of cancer-associated mortality worldwide. MicroRNAs (miRNAs/miRs) serve a role in the occurrence and development of lung cancer. The aim of the present study was to analyze the expression and function of the proliferation-associated miR-383-5p in lung adenocarcinoma (LAC). Samples of human LAC and matched adjacent normal lung tissues were surgically removed, and miR-383-5p expression and the pathological characteristics of lung adenocarcinoma were investigated. The present study revealed that miR-383-5p expression level was significantly decreased in LAC tissues and its expression levels were markedly associated with tumor size and differentiation...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927105/reactive-oxygen-species-driven-mitochondrial-injury-induces-apoptosis-by-teroxirone-in-human-non-small-cell-lung-cancer-cells
#10
Jing-Ping Wang, Chang-Heng Hsieh, Chun-Yen Liu, Kai-Han Lin, Pei-Tsun Wu, Kwun-Min Chen, Kang Fang
Teroxirone as an anticancer agent is used to treat human lung cancer by inducing apoptotic cell death. Previous studies have demonstrated that the status of the tumor suppressor p53 determined the onset of apoptotic cell death in human non-small cell lung cancer cells (NSCLC). In order to further understand the underlying mechanisms of lung cancer, the present study explored the targets of teroxirone. By including antioxidants, the present study analyzed changes in cell proliferation, cell cycle division, mitochondrial membrane potential (MMP), reactive oxygen species (ROS), expression of apoptosis markers and cytochrome c distribution...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927092/synergistic-anticancer-effect-of-curcumin-and-chemotherapy-regimen-fp-in-human-gastric-cancer-mgc-803-cells
#11
Bin He, Wen Wei, Ji Liu, Yundan Xu, Gang Zhao
Curcumin is an anticancer compound that exerts anti-proliferative and apoptotic effects via multiple molecular targets. The purpose of the present study was to investigate the anticancer effects of curcumin in combination with 5-fluorouracil plus cisplatin (FP) on the MGC-803 human gastric cancer cell line. Following treatment with curcumin and/or FP for 24, 48 and 72 h, cell viability, cell cycle progression and the apoptosis rate were evaluated using an MTT assay, flow cytometry and dual acridine orange/ethidium bromide staining, respectively...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927086/quercetin-inhibits-angiogenesis-mediated-human-retinoblastoma-growth-by-targeting-vascular-endothelial-growth-factor-receptor
#12
Wei Song, Xiaofei Zhao, Jiarui Xu, Han Zhang
Retinoblastoma (RB) is the most common malignant intraocular cancer in teenagers, occurrence of which depends on the mutation of multiple genes. Among all the signaling pathways involved in the oncogenesis of RB, the process of angiogenesis has been demonstrated to be associated with the local invasive growth and metastasis of this cancer type. Quercetin (Que) is a typical flavonoid and has been reported to inhibit angiogenesis in various types of tumors. In the present study, the effect of Que on RB cells and angiogenesis of RB was evaluated...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927069/overexpression-of-reic-dkk-3-suppresses-the-expression-of-cd147-and-inhibits-the-proliferation-of-human-bladder-cancer-cells
#13
Yuhei Horikawa, Masami Watanabe, Takuya Sadahira, Yuichi Ariyoshi, Yasuyuki Kobayashi, Motoo Araki, Koichiro Wada, Kazuhiko Ochiai, Shun-Ai Li, Yasutomo Nasu
Our group previously developed an adenoviral vector encoding the REIC/Dkk-3 gene (Ad-REIC), a tumor suppressor, for cancer gene therapy. The Ad-REIC agent induces apoptosis and inhibits invasion in a number of cancer cell lines; however, the molecular mechanisms underlying its effects remain unclear. Cluster of differentiation 147 (CD147), also known as extracellular matrix metalloproteinase inducer (EMMPRIN), is a key molecule that promotes cancer proliferation and invasion. In order to elucidate the therapeutic mechanism of Ad-REIC, its effect on the expression of CD147 in human bladder cancer KK47 cells was investigated...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927059/matrine-inhibits-cell-proliferation-and-induces-apoptosis-of-human-rhabdomyosarcoma-cells-via-downregulation-of-the-extracellular-signal-regulated-kinase-pathway
#14
Penghui Yan, Zongqiang Huang, Junbo Zhu
Matrine, extracted from the Chinese traditional medicine Sophorae flavescentis, has been demonstrated to exhibit antitumor effects on numerous types of cancer in vivo and in vitro with low toxicity. However, its antitumor mechanism in rhabdomyosarcoma (RMS) cells remains unclear. In the present study, the antitumor effects of matrine and its underlying mechanisms in RMS were investigated in vitro. The results demonstrated that matrine inhibited cell proliferation, migration and invasion, and induced apoptosis of RMS cells in a dose-dependent manner...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927053/synthesis-biological-evaluation-and-mechanism-studies-of-matrine-derivatives-as-anticancer-agents
#15
Lihe Jiang, Lichuan Wu, Fangfang Yang, Nadin Almosnid, Xu Liu, Jun Jiang, Elliot Altman, Lisheng Wang, Ying Gao
A total of five matrine derivatives were synthesized and evaluated for their anti-proliferation activity using a panel of four human cancer cell lines, including A549 lung, BT20 breast, MCF-7 breast and U2OS osteosarcoma cells. The YF3-5, YF3-7 and YF3-9, three novel compounds, demonstrated increased anti-proliferation activity compared with matrine, of which YF3-5 revealed the strongest anti-proliferation activity with a half-maximal inhibitory concentration value of 15.49-16.67 µM against the four human cancer cell lines...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927034/cyclin-d3-deficiency-inhibits-skin-tumor-development-but-does-not-affect-normal-keratinocyte-proliferation
#16
Sung Hyun Lee, Xian Wang, Sun Hye Kim, Yongbaek Kim, Marcelo L Rodriguez-Puebla
Rearrangement and amplification of the D-type cyclin genes have been reported in human cancer. Previous studies have demonstrated that Ras-mediated skin tumorigenesis depends on pathways that act through cyclin D1 and D2; however, the role of cyclin D3 remains unknown. The present study demonstrates that cyclin D3 ablation does not affect keratinocyte proliferation, but instead increases apoptosis levels in the bulge region of the hair follicle. Consequently, cyclin D3 ablation reduces skin papilloma development in a Ras-dependent carcinogenesis model...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28926932/comparison-of-two-components-of-propolis-caffeic-acid-ca-and-caffeic-acid-phenethyl-ester-cape-induce-apoptosis-and-cell-cycle-arrest-of-breast-cancer-cells-mda-mb-231
#17
Agata Kabała-Dzik, Anna Rzepecka-Stojko, Robert Kubina, Żaneta Jastrzębska-Stojko, Rafał Stojko, Robert Dariusz Wojtyczka, Jerzy Stojko
Studies show that caffeic acid (CA) and caffeic acid phenethyl ester (CAPE) are compounds with potent chemopreventive effects. Breast cancer is a common form of aggressive cancer among women worldwide. This study shows a comparison of CA and CAPE activity on triple-negative human caucasian breast adenocarcinoma line cells (MDA-MB-231). MDA-MB-231 cells were treated by CA and CAPE with doses of from 10 to 100 µM, for periods of 24 h and 48 h. Cytotoxicity MTT tests, apoptosis by Annexin V, and cell cycle with Dead Cell Assays were performed...
September 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28926928/curcumin-inhibited-growth-of-human-melanoma-a375-cells-via-inciting-oxidative-stress
#18
Wang Liao, Wei Xiang, Fei-Fei Wang, Rui Wang, Yan Ding
Curcumin, a polyphenol compound, possesses potent pharmacological properties in preventing cancers, which make it as a potential anti-cancer mediator. However, it is still unknown that whether Curcumin induced melanoma A375 cell was associated with oxidative stress. Here, we firstly found a fascinating result that Curcumin could reduce the proliferation and induced apoptosis of human melanoma A375 cells. Meanwhile, IC50 of Curcumin on A375 cells is 80μM at 48h. In addition, Curcumin caused oxidative stress through inducing further ROS burst, decreasing GSH, and wrecking mitochondria membrane potential (MMP), which were reversed by ROS inhibitor N-acetylcysteine (NAC)...
September 15, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28926527/genistein-suppresses-aerobic-glycolysis-and-induces-hepatocellular-carcinoma-cell-death
#19
Sainan Li, Jingjing Li, Weiqi Dai, Qinghui Zhang, Jiao Feng, Liwei Wu, Tong Liu, Qiang Yu, Shizan Xu, Wenwen Wang, Xiya Lu, Kan Chen, Yujing Xia, Jie Lu, Yingqun Zhou, Xiaoming Fan, Wenhui Mo, Ling Xu, Chuanyong Guo
BACKGROUND: Genistein is a natural isoflavone with many health benefits, including antitumour effects. Increased hypoxia-inducible factor 1 α (HIF-1α) levels and glycolysis in tumour cells are associated with an increased risk of mortality, cancer progression, and resistance to therapy. However, the effect of genistein on HIF-1α and glycolysis in hepatocellular carcinoma (HCC) is still unclear. METHODS: Cell viability, apoptosis rate, lactate production, and glucose uptake were measured in HCC cell lines with genistein incubation...
September 19, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28926122/stem-cells-and-differentiated-cells-differ-in-their-sensitivity-to-urine-in-vitro
#20
Marta Pokrywczyńska, Tomasz Kloskowski, Daria Balcerczyk, Monika Buhl, Arkadiusz Jundziłł, Maciej Nowacki, Kaja Męcińska-Jundziłł, Tomasz Drewa
INTRODUCTION: Urinary tract regeneration using tissue engineering is one of the most challenging issues in the field of reconstructive urology. Cells seeded on scaffold are exposed to urine immediately after the implantation. The outcome of urinary bladder regeneration is depended on the ability of these cells to survive, proliferate and regenerate. The aim of this study was to compare a sensitivity of three different cell lines to urine in vitro. MATERIAL AND METHODS: Three different cell lines were isolated from porcine bladder (urothelial cells, UCs and smooth muscle cells, SMCs) and adipose tissue (adipose-derived stem cells, ADSCs)...
September 19, 2017: Journal of Cellular Biochemistry
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