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K Polakova, E Bandzuchova
HLA-G antigens and matrix metalloproteinases (MMPs) expressed in various tumors are involved in tumor growth and metastasis. In this study we investigated whether in cell lines of different origin exist some correlation between HLA-G and MMP expression. First, we examined MMP transcription in two choriocarcinoma cell lines: JEG-3 (HLA-G positive) and JAR (HLA-G negative). We discovered that both cell lines express similar panel of MMPs except MMP12. Transcript MMP12 was exclusively detected in HLA-G expressing JEG-3 cells but not in HLA-G deficient JAR cells...
September 4, 2018: Neoplasma
Jun Shen, Cao Yin, Xiao Jiang, Xuan Wang, Shujuan Yang, Guangbao Song
BACKGROUNDS: To investigate alterations in histone modification and histone deacetylases (HDACs) in patients with oral lichen planus (OLP), and to evaluate correlations with inflammatory cytokine production. METHODS: Global histone H3/H4 acetylation and HDAC activity in CD4+ T cells from 23 patients with OLP and 10 healthy control subjects were examined using spectrophotometry. The mRNA levels of 8 members of 4 classes of HDAC genes were measured by real-time quantitative polymerase chain reaction...
October 17, 2018: Journal of Oral Pathology & Medicine
Yuqin Zhang, Guohong Yan, Chengtao Sun, Lihong Nan, Xiaoying Wang, Wei Xu, Kedan Chu
Compound 3R-(4'-hydroxyl-3'-O-β-D-glucopyranosyl phenyl)-dihydro isocoumarin (GDC) is a natural isocoumarin, recently isolated from the stems of H. paniculiflorum. However, we know little about the effects of GDC on rheumatoid arthritis (RA). This study aims to investigate the protective effects and potential mechanisms of GDC against LPS-induced inflammation in vitro. Fibroblast-like synoviocytes (FLSs) obtained from synovial tissue of rats were induced by lipopolysaccharide (LPS) and treated with GDC. Cell viability was determined by mitochondrial-respiration-dependent3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay...
October 17, 2018: Inflammation
Rajkiran Reddy Banala, Satish Kumar Vemuri, Murahari Penkulinti, Gurava Reddy Av, Subbaiah Gpv
Study Design: To induce scoliosis in young female Wistar rats using a noninvasive method and to validate this model. Purpose: To induce scoliosis in a rat model noninvasively by bracing and to study the corresponding gene-expression profile in the spine and different organs. Overview of Literature: Scoliosis involves abnormal lateral curvature of the spine, the causes of which remain unclear. In the literature, it is suggested that scoliosis is genetically heterogeneous, as there are multiple factors involved directly or indirectly in its pathogenesis...
October 18, 2018: Asian Spine Journal
Mohamed A Ibrahim, Asmaa Abdel-Aziz, Azza El-Sheikh, Maha Kamel, Al-Zahraa Khalil, Hisham Abdelhaleem
Introduction: The study aimed at investigating the hepatic effect of direct acting anti-hepatitis C virus drugs (DAAs), sofosbuvir (Sof) and daclatasvir (Dac), in thioacetamide (TAA)-induced liver injury in rats. Material and methods: Animals were allocated to 7 groups: a normal control group, a TAA group (receiving TAA 50 mg/kg, i.p. twice weekly), two TAA groups receiving either a low or a high dose of Sof (Sof-L and Sof-H; 41.1 mg/kg and 82.2 mg/kg, respectively), two TAA groups receiving either a low or a high dose of Dac (Dac-L and Dac-H; 6...
September 2018: Clinical and Experimental Hepatology
Lech Romanowicz, Tomasz Gogiel, Zofia Galewska, Marta Bruczko, Justyna Bączyk, Wiesława Roszkowska-Jakimiec, Krzysztof Sobolewski
OBJECTIVES: Preeclampsia is the most common disorder associated with pregnancy. Our earlier findings revealed a substantial increase in the amount of matrix metalloproteinase-26 (matrilysin 2; MMP-26) in preeclamptic umbilical cord blood. The role of MMP-26 in preeclamptic umbilical cord tissue has not been fully elucidated. Some reports have indicated that the expression of matrilysin 2 and tissue inhibitor of matrix metalloproteinase 4 (TIMP-4) is coordinately regulated during progression of various diseases...
October 9, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Sonia Aloui, Wiem Zidi, Sana Ouali, Imen Guizani, Sameh Hadj-Taieb, Mohamed Sami Mourali, Moncef Feki, Monia Allal-Elasmi
Calcific mitral valve stenosis (MVS) is a common disease characterized by extensive remodeling of the extracellular matrix via matrix metalloproteinases (MMPs). The mechanism of calcification due to extensive matrix remodeling remains unclear. In this study, we investigated the relationship between MMP-3, tissue inhibitors of metalloproteinases (TIMPs) as well as pro-inflammatory cytokines and the phenomenon of calcification in MVS. 212 patients having rheumatic mitral stenosis (RMS) and 155 healthy control subjects were recruited in the Cardiology Department of La Rabta Hospital University...
October 9, 2018: Molecular Biology Reports
Jonna R Bank, Renée Ruhaak, Darius Soonawala, Oleg Mayboroda, Fred P Romijn, Cees van Kooten, Christa M Cobbaert, Johan W de Fijter
BACKGROUND: Urinary tissue inhibitor of metalloproteinases-2 (TIMP-2) and insulin-like growth factor binding protein-7 (IGFBP7) have been validated as biomarkers for acute kidney injury. We investigated the performance of both markers in predicting the occurrence and duration of functionally defined delayed graft function (fDGF) in donation after circulatory death (DCD) kidney transplant recipients. METHODS: Urine samples of 74 DCD recipients were analyzed. TIMP-2 and IGFBP7 were measured with ELISA on postoperative day 1 to 7, day 10, week 6 and month 6 and values were corrected for osmolality (mOsm)...
October 8, 2018: Transplantation
Sheng-Teng Huang, Cheng-Chieh Chang, Jong-Hwei S Pang, Hung-Sen Huang, Shen-Chieh Chou, Ming-Ching Kao, Huey-Ling You
Background and Purpose: Drynaria fortunei J. Sm ( D. fortunei ), known as Gu-Sui-Bu, is used in traditional Chinese medicine to treat common injuries, including bone fractures and bruising. The specific functional mechanisms of the angiogenic and endothelial cell migration properties of D. fortunei are currently unclear. Thus, the purpose of this study is to validate the potential angiogenic and cellular migration properties and related mechanisms by D. fortunei both in vivo and in vitro . Experimental Approach: The present study investigates, both in vivo and in vitro , the wound healing effects of D...
2018: Frontiers in Pharmacology
Alessia Gallo, Valentina Agnese, Claudia Coronnello, Giuseppe M Raffa, Diego Bellavia, Pier Giulio Conaldi, Michele Pilato, Salvatore Pasta
BACKGROUND: To determine the impact of circulating miRNA and protein activity on the severity of ascending aortic dilatation in patients with bicuspid (BAV) and tricuspid aortic valve (TAV). METHODS: By reverse transcription polymerase chain reaction, exosomal circulating expression levels (versus healthy aorta) of miRNAs and absolute levels of transforming growth factor β (TGF-β), matrix metalloproteinases (MMP-2, -3 and -9), tissue inhibitors (TIMP-1, -2, -3 and -4), and soluble receptors for advanced glycation end products AGEs (sRAGE) were evaluated in ascending dilated aortas of 71 patients with different valve morphotype...
October 5, 2018: International Journal of Cardiology
Katarzyna Oszajca, Maciej Szemraj, Janusz Szemraj, Piotr Jurowski
BACKGROUND: Age-related macular degeneration is a progressive eye disease affecting the macula and causing acute visual loss particularly in elder people. The aim of the study was an attempt to discern an influence of expression levels and functional genetic polymorphisms of selected genes related to the extracellular matrix turnover or neovascularization on age-related macular degeneration occurrence and progression. METHODS: We conducted a case-control study of 200 polish patients with recognized age-related macular degeneration (dry and wet) and compared the results with those obtained from matched 100 healthy control subjects...
October 5, 2018: Ophthalmic Genetics
Marie Krogh Nielsen, Yousif Subhi, Christopher Rue Molbech, Line Lynge Nilsson, Mogens Holst Nissen, Torben Lykke Sørensen
PURPOSE: Tissue inhibitor of metalloproteinase (TIMP) is known to play a role in age-related macular degeneration (AMD). We wished to investigate alterations in different late stages of AMD: neovascular AMD and geographic atrophy (GA). METHODS: This was a prospective case-control study. A total of 125 participants were included consecutively during a period of 18 months. We included 46 patients with neovascular AMD, 46 patients with GA without any sign of choroidal neovascularization in either eye, and 33 healthy aged controls...
October 4, 2018: Acta Ophthalmologica
Virender Kumar, Vinod Kumar, Jiangtao Luo, Ram I Mahato
Trans-differentiation of quiescent hepatic stellate cells (HSCs) into active myofibroblasts secretes excess amounts of extracellular matrix (ECM) proteins. miR-29b1 has the potential to treat liver fibrosis, because it targets several profibrotic genes. We previously demonstrated that miR-29b1 and the hedgehog (Hh) pathway inhibitor GDC-0449 could, together, inhibit the activation of HSCs and ECM production in common bile-duct-ligated (CBDL) mice. Herein, we determined the effect of chemical modifications of miR-29b1 on its stability, immunogenicity, and Argonaute-2 (Ago2) loading in vitro, after modifying its antisense strand with phosphorothioate (PS-miR-29b1), 2'-O-methyl-phosphorothioate (OMe-miR-29b1), locked nucleic acid (LNA-miR-29b1), and N,N'-diethyl-4-(4-nitronaphthalen-1-ylazo)-phenylamine (ZEN-miR-29b1)...
September 1, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
Yangming Liu, Nan Shan, Yu Yuan, Bin Tan, Chengjin He, Chao Tong, Hongbo Qi
INTRODUCTION: The sufficient invasion and migration of human extravillous trophoblast (EVTs) cells are crucial for placentation. Inadequate invasion of trophoblasts may correlate with the development of preeclampsia. Many studies have suggested that activated Cdc42-associated kinase (ACK1) is associated with tumor metastasis and invasion. This study investigated the ACK1 expression and its function in trophoblasts during placental development. METHODS: ACK1 expression in human placentas was determined through immunofluorescence...
October 3, 2018: Journal of Maternal-fetal & Neonatal Medicine
Holly Corbitt, Shaine A Morris, Claus H Gravholt, Kristian H Mortensen, Rebecca Tippner-Hedges, Michael Silberbach, Cheryl L Maslen
Turner syndrome is caused by complete or partial loss of the second sex chromosome, occurring in ~1 in 2,000 female births. There is a greatly increased incidence of aortopathy of unknown etiology, including bicuspid aortic valve (BAV), thoracic aortic aneurysms, aortic dissection and rupture. We performed whole exome sequencing on 188 Turner syndrome participants from the National Registry of Genetically Triggered Thoracic Aortic Aneurysms and Cardiovascular Related Conditions (GenTAC). A gene-based burden test, the optimal sequence kernel association test (SKAT-O), was used to evaluate the data with BAV and aortic dimension z-scores as covariates...
October 2018: PLoS Genetics
H-Y Li, F Liu, C Gao, H-R Wang
OBJECTIVE: To study the protective effect of simvastatin on arterial plaque instability induced by p-cresyl sulfate (PCS). MATERIALS AND METHODS: Apolipoprotein E (ApoE)-/- mice were selected as objects of this study. All mice were randomly divided into three groups: 1) the control group, 2) the PCS group and 3) the PCS + simvastatin group. After successful modeling, the levels of plasma cholesterol, triglyceride, low-density lipoprotein, high-density lipoprotein, interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor-β (TNF-β) were detected...
September 2018: European Review for Medical and Pharmacological Sciences
Quan Wang, Yilin Shen, Bin Ye, Haixia Hu, Cui Fan, Tan Wang, Yuqin Zheng, Jingrong Lv, Yan Ma, Mingliang Xiang
To identify differences in gene expression profiles of infected cells between thyroid carcinoma (C), thyroid adenoma (A) and normal thyroid (N) epithelial cells, differentially expressed genes were identified using three pairwise comparisons with the GEO2R online tool. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were used to classify them at the functional level. The most significant cluster in the N vs. A pairwise comparison had four hub genes: Insulin-like growth factor 2, Von Willebrand factor (VWF), multimerin 1 (MMRN1) and complement factor D (CFD)...
September 20, 2018: Oncology Reports
Diaa E E Rizk, Ghada Al-Kafaji, Ahmed A Jaradat, Diab Al-Tayab, Moiz Bakhiet, Stefano Salvatore
The purpose of the present study was to measure the plasma levels of matrix metalloproteinases (MMP)-2 and -9 and their tissue inhibitors (TIMP)-1 and -2, as surrogate biomarkers for pelvic floor tissue integrity in young, healthy multi-ethnic women. This was hoped to elucidate ethnic vulnerability to support-related pelvic floor dysfunctions. The plasma levels of MMP-2 and -9 and TIMP-1 and -2 were measured by sandwich ELISA in nulliparous, young (18-29 years) women volunteers (n=85) from five ethnic groups [n=17/group; Bahrainis, other Arabs, Filipinos, Indians/Pakistanis and Caucasians (Italians)] and compared with levels in Italians as the reference group...
September 2018: Biomedical Reports
HyeongSeob Kwak, Hongyan An, Md Badrul Alam, Won-Sik Choi, Sang Yong Lee, Sang-Han Lee
β-Escin, a natural triterpene saponin was extracted from Aesculus hippocastanum seeds, which have been widely used to treat inflammation in traditional medicine. In an effort to study the possible anti-tumor effects of β-escin, we performed wound healing, invasion, and adhesion assays to examine the effects of β-escin on cell migration, invasion, and angiogenesis. Our results revealed that β-escin inhibits cell migration as well as motility in B16F10 and SK-MEL5 cells in a dose-dependent manner. RT-PCR and Western blot analysis showed that β-escin increased TIMP-1, -2 while significantly downregulated phosphorylated extracellular signal-regulated kinase (p-ERK) expression, and suppressing nuclear factor-kappa B (NF-κB) and inhibitor of nuclear factor-kappa B (IκB) expression...
2018: Biological & Pharmaceutical Bulletin
Finosh G Thankam, Daugherty K Evan, Devendra K Agrawal, Matthew F Dilisio
Tendonosis is characterized by an increase in collagen type III relative to collagen type I, but the precise ratio of collagen type I to collagen type III in pathologic shoulder tendons has not been reported. A tendon continuously bathed in an OA synovial environment may exhibit histomorphologic differences as compared to those from shoulders with a preserved articular surface. The purpose of this study was to evaluate the biochemical and histological differences in the proximal portion of the long head of the biceps tendon (LHBT) from a non-arthritic versus an arthritic glenohumeral joint...
September 28, 2018: Molecular and Cellular Biochemistry
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