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Chetan Kumar, Reyaz Ur Rasool, Zainab Iqra, Yedukondalu Nalli, Prabhu Dutt, Naresh K Satti, Neha Sharma, Sumit G Gandhi, Anindya Goswami, Asif Ali
Herein, we report the isolation and synthetic modification of dehydrozingerone (DHZ, 1 ), a secondary metabolite present in the rhizome of Zingiber officinale . We synthesized O -propargylated dehydrozingerone, which was subsequently coupled by alkyne-azide cycloaddition ( 3-20 ) using click chemistry. The compounds ( 1-20 ) were evaluated for their in vitro cytotoxic activity in a panel of three cancer cell lines. Among all the DHZ derivatives, 3 , 6 , 7 , 8 , 9 and 15 displayed potent cytotoxic potential with an IC50 value ranging from 1...
November 1, 2017: MedChemComm
Cornel Balta, Alina Ciceu, Hildegard Herman, Marcel Rosu, Oana Maria Boldura, Anca Hermenean
Liver fibrosis represents an overaccumulation of extracellular matrix (ECM). This study was designed to investigate the effect of chrysin on established ECM overproduction in carbon tetrachloride (CCl4 ) mouse liver fibrosis. Experimental fibrosis was induced by intraperitoneal injection of 2 mL/kg CCl4 twice a week, for 7 weeks. Mice were orally treated with 3 doses of chrysin (5,7-dihydroxyflavone). For the assessment of the spontaneous reversion of fibrosis, CCl4 -treated mice were investigated after 2 weeks of recovery time...
July 2018: Dose-response: a Publication of International Hormesis Society
Zijie Mei, Xiaoli Tian, Jing Chen, Yacheng Wang, Ye Yao, Xin Li, Chunxu Yang, Shimin Zhang, Conghua Xie
Radiation‑induced lung injury (RILI) is a major complication of thoracic radiotherapy that starts as exudative inflammation and proceeds to lung fibrosis, and additional studies are required to develop methods to ameliorate RILI. The aim of this study was to explore whether the nicotinic acetylcholine receptor subtype‑7 (α7‑nAChR) agonist GTS‑21 has a protective effect against RILI. C57BL6 mice were irradiated with 12 Gy to induce a mouse model of RILI. Some of the mice received an i.p. injection of 4 mg/kg GTS‑21 for three days with or without radiation treatment...
August 1, 2018: Oncology Reports
I Radziejewska, K Supruniuk, J Nazaruk, E Karna, B Popławska, A Bielawska, A Galicka
Rosmarinic acid (RA) is a natural phenylpropanoid with numerous pharmacological activities. Because of limited studies of the effects of RA action in gastric cancer cells we examined how 100 and 200 μM acid influences MMPs, TIMPs, collagen, MUC1 and specific sugar antigens in gastric adenocarcinoma CRL-1739 cells. We revealed inhibitory effect of RA on MMP-9 activity what was correlated with increased collagen type I expression, main ECM substrate degraded by MMPs. Tissue inhibitor of MMPs, TIMP-1 but not TIMP-2 was significantly decreased on the protein level and increased on mRNA level by RA action what can suggest TIMP-1 independent inhibitory action of an acid on MMP-9 activity...
August 9, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Wei Wang, Jiye Wang
BACKGROUND Toll-like receptor 4 (TLR4)-mediated signaling has been implicated in invasion, metastasis, and survival of various cancers. Activation of TLR4 can promote cyclooxygenase-2 (COX-2) and nuclear factor-κB (NF-κB). However, little is known about the effects of TLR4/COX-2 in prostate cancer (PCa). MATERIAL AND METHODS In our study, TLR4 and COX-2 expressions were detected by quantitative real-time reverse transcription PCR (qRT-PCR) in PCa tissues (n=34). Cell proliferation was measured by Cell Counting Kit-8 (CCK-8) and carboxyfluorescein succinimidyl ester (CFSE) assays...
August 11, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Xiaobin Guo, Liangliang Wang, Menglei Xu, Jiaxiang Bai, Jining Shen, Binqing Yu, Yu Liu, Houyi Sun, Yuefeng Hao, Dechun Geng
Abnormal reduction of extracellular matrix (ECM), including type II collagen and aggrecan, caused by tumor necrosis factor-α (TNF-α) is an important pathological feature of osteoarthritis (OA). Shikimic acid (SA), derived from natural plants, has displayed effective pharmacological properties in diverse diseases. The biological roles of SA in OA have not been reported before. Here, we found that treatment with SA (1 mM, 10 mM) prevented TNF-α-induced degradation of type II collagen and aggrecan ECM in human primary chondrocytes culture in vitro...
August 6, 2018: International Immunopharmacology
Yi Chen, Jiahui Wei, Ying Zhang, Wenwei Sun, Zhuoheng Li, Qin Wang, Xiaoyu Xu, Cong Li, Panhong Li
Jiawei Foshou San (JFS) is the new formula originated from classic Foshou San formula, composed with ligustrazine, ferulic acid, and tetrahydropalmatine. Previously JFS inhibited the growth of endometriosis (EMS) with unclear mechanism, especially in metastasis, invasion, and epithelial-mesenchymal transition. In this study, network pharmacology was performed to explore potential mechanism of JFS on EMS. Through compound-compound target and compound target-EMS target networks, key targets were analyzed for pathway enrichment...
2018: Frontiers in Pharmacology
Odin Goovaerts, Pauline N M Mwinzi, Erick M O Muok, Ann Ceulemans, Robert Colebunders, Luc Kestens
BACKGROUND: Among the different faces of immune reconstitution inflammatory syndrome (IRIS) developing in HIV-patients, no clinical definition has been reported for Schistosomiasis-IRIS (Schisto-IRIS). Although Schisto-IRIS remains largely uninvestigated, matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP) have previously been associated with S. mansoni infection and tuberculosis-IRIS. Here, we aimed to investigate the relevance of these markers in Schisto-IRIS...
August 8, 2018: PLoS Neglected Tropical Diseases
T N Enina, V A Kuznetsov, A M Soldatova, T I Petelina, D V Krinochkin, S M Dyachkov, A Y Rychkov, T Y Gorbunova
AIM: To study the relationship between levels of sex hormones and effectiveness of cardiac resynchronisation therapy (CRT) in men with chronic heart failure (CHF). MATERIALS AND METHODS: The best response to CRT (mean time, 38 [19,0;53,7] months) was identifed by a maximum decrease in left ventricular end-systolic volume (LVESV) in 58 men (mean age, 54.8±9.6) with CHF (61% IHD). Based on testosterone (TES) level, patients were divided into group 1 (n=28; 48%) - TES < median value (13...
2018: Kardiologiia
Shweta Reddy, Prity Sahay, Debananda Padhy, Sarada Sarangi, Mrutyunjay Suar, Rahul Modak, Aparna Rao
PURPOSE: Prostaglandin analogues (PGA's) are the mainstay and first line of treatment in current glaucoma practise. Though latanoprost and bimatoprost are the most commonly used PGA's with minimal side effects at lower concentrations like bimaotoprost 0.01%, direct comparison of their cytokine/MMP profile in tears has not been evaluated earlier. The study intends to ascribe PGA to the upregulation of MMPs, Cytokines and Chemokines mediating varied pathways to result in side effects of the drugs...
2018: PloS One
Tram T Vuong, Sissel B Rønning, Tamer A E Ahmed, Kristiane Brathagen, Vibeke Høst, Maxwell T Hincke, Henri-Pierre Suso, Mona E Pedersen
Avian eggshell membrane (ESM) is a natural biomaterial that has been used as an alternative natural bandage to cure wounds, and is available in large quantities from egg industries. We have previously demonstrated that processed eggshell membrane powder (PEP), aiming to be used in a low cost wound healing product, possesses anti-inflammatory properties. In this study, we further investigated effects of PEP on MMP activities in vitro (a dermal fibroblast cell culture system) and in vivo (a mouse skin wound healing model)...
2018: PloS One
L B Kim, G S Russkih, A N Putyatina, O B Tsypysheva
Here we present the study of enzymes involved in the regulation of extracellular matrix metabolism. It was the first study of this kind in the European part of the Arctic zone of the Russian Federation (AZRF). The contents of matrix metalloproteinases (MMP-1, -2, -3, -9) and tissue inhibitors of matrix metalloproteinases (TIMP-1, -2, -4) were measured in blood plasma of 91 men in AZRF (67o N) and 14 men in Western Siberia. The contents of MMP-1, MMP-9, TIMP-1 and TIMP-4 in plasma of northerners were higher compared to those of the residents of Western Siberia (55o N)...
2018: Advances in Gerontology, Uspekhi Gerontologii
Khalil Khanafer, Abhijit Ghosh, Kambiz Vafai
OBJECTIVE: The objective of this preliminary investigation is to determine if there is a relation between the biological levels of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinase (TIMP) and the elastic moduli of the ascending aortic wall in patients with ascending thoracic aortic aneurysms (ATAA). METHODS: Circumferential specimens from twelve patients with ATAA were obtained from the greater curvature and their tensile properties (maximum elastic modulus) were tested uniaxially...
July 7, 2018: Cardiovascular Revascularization Medicine: Including Molecular Interventions
Priyanka Banerjee, Ankita Malik, Sudha Saryu Malhotra, Satish Kumar Gupta
During pregnancy, regulated generation of reactive oxygen species (ROS) is important for activation of signaling pathways and placentation. In the current study, the effect of H2 O2 on invasion of HTR-8/SVneo cells, a human extravillous trophoblast cell line, is investigated. Treatment of HTR-8/SVneo cells for 24 hr with H 2 O2 (25 µM) leads to a significant increase in invasion without affecting cell proliferation, viability, and apoptosis. Concomitantly, a significant increase in the matrix metalloproteinase-9 (MMP-9)/tissue inhibitor of metalloproteinases-1 (TIMP-1) ratio is observed...
August 5, 2018: Journal of Cellular Physiology
Yan Huang, Gang Li, Kai Wang, Zhongyi Mu, Qingpeng Xie, Hongchen Qu, Hang Lv, Bin Hu
BACKGROUND Collagen type VI alpha 3 chain (COL6A3) has been proven to be a biomarker in the occurrence and development of bladder cancer, which is the most common malignant tumor in the urinary system. This study aimed to explore the effect and molecular mechanism of COL6A3 on EMT in vitro induced by TGF-β/Smad in bladder carcinoma. MATERIAL AND METHODS There were 42 patients included in the Kaplan-Meier survival analysis. A cell counting kit-8 (CCK-8) assay and an angiogenesis assay were used to measure cell proliferation and tube formation, respectively...
August 1, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
De-Bin Guo, Xiang-Qing Zhu, Qing-Qing Li, Gao-Mi-Yang Liu, Guang-Ping Ruan, Rong-Qing Pang, Yu-Hao Chen, Qiang Wang, Jin-Xiang Wang, Ju-Fen Liu, Qiang Chen, Xing-Hua Pan
Umbilical cord mesenchymal stem cells (UC-MSCs) exert strong immunomodulatory effects and can repair organs. However, their roles in radiation injury remain unclear. We show that in tree shrews with acute radiation injury, injected UC-MSCs significantly improved survival rates, reduced lung inflammation and apoptosis, prevented pulmonary fibrotic processes, recovered hematopoiesis, and increased blood counts. A protein microarray analysis showed that serum levels of the anti-inflammatory cytokines IL-10 and IL-13 and the growth factors BMP-5, BMP-7, HGF, insulin, NT-4, VEGFR3, and SCF were significantly higher, while those of the inflammatory cytokines IL-2, TIMP-2, TNF-α, IFN-γ, IL-1ra, and IL-8 and the fibrosis-related factors PDGF-BB, PDGF-AA, TGF-β1, IGFBP-2, and IGFBP-4 were significantly lower in UC-MSC-injected animals...
July 31, 2018: Cytotechnology
Konrad A Szychowski, Anna K Wójtowicz, Jan Gmiński
Degradation products of elastin, i.e. elastin-derived peptides (EDPs), are involved in various physiological and pathological processes. EDPs are detectable in cerebrospinal fluid in healthy people and in patients after ischemic stroke. However, to date, no studies concerning the role of EDP in the nervous system were conducted. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) play important roles during the repair phases of cerebral ischemia, particularly during angiogenesis and reestablishment of cerebral blood flow...
July 30, 2018: Neurotoxicity Research
Xiao-Li Yuan, Chun-Sheng Li, Yun Zhang, Ji-Yang Ling, Qiang Zhang, Yong Liang, Bo Liu, Lian-Xing Zhao
Background: Acute kidney injury (AKI) frequently occurs in cardiopulmonary resuscitation patients. Studies comparing the effects of extracorporeal membrane oxygenation (ECMO) with conventional cardiopulmonary resuscitation (CCPR) on AKI were rare. This study aimed to compare the effects of ECMO with those of CCPR on survival rate and AKI and explore the underlying mechanisms in a swine model of cardiac arrest (CA). Methods: Sixteen male pigs were treated with ventricular fibrillation to establish CA model and then underwent CCPR (CCPR group, n = 8) or ECMO during cardiopulmonary resuscitation (ECPR group, n = 8)...
August 5, 2018: Chinese Medical Journal
Suzy M Salama, Ibrahim Abdel Aziz Ibrahim, Naiyer Shahzad, Saeed Al-Ghamdi, Nahla Ayoub, Ahmed Salim AlRashdi, Mahmood Ameen Abdulla, Nur'Ain Salehen, Mehmet Bilgen
This experiment evaluated Panduratin A (PA), a chalcone isolated from Boesenbergia rotunda rhizomes, for its hepatoprotectivity. Rats were subjected to liver damage induced by intra-peritoneal injection of thioacetamide (TAA). PA was tested first for its acute toxicity and then administered by oral gavage at doses 5, 10, and 50 mg/kg to rats. At the end of the 8th week, livers from all rats were excised and evaluated ex vivo. Measurements included alkaline phosphatase (AP), alanine transaminase (ALT), aspartate transaminase (AST) and gamma-glutamyl transferase (GGT), serum platelet-derived growth factor (PDGF) and transforming growth factor (TGF-β1), and hepatic metalloproteinase enzyme (MMP-2) and its inhibitor extracellular matrix protein (TIMP-1)...
July 29, 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
Cai-Xia Li, Li-Hua Cui, Yu-Zhen Zhuo, Jian-Gong Hu, Nai-Qiang Cui, Shu-Kun Zhang
AIMS: Autophagy is an intracellular metabolic process that degrades and recycles own constituents to maintain homeostasis and supply substrates. Disruption of collagen degradation is one of the pathogenesis of pancreatic fibrosis. In this study, we investigated the effects of inhibiting autophagy on the collagen degradation of PSCs. MAIN METHODS: Rats were injected dibutyltin dichloride (DBTC) to induce chronic pancreatitis (CP) model. The expression of LC3B was measured by western blotting...
July 26, 2018: Life Sciences
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