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Zichao Wang, Peizhang Chen, Ning Tao, Huiru Zhang, Ruifang Li, Xiaobei Zhan, Fuzhuan Wang, Yingben Shen
Two polysaccharides were produced by Chaetomium globosum CGMCC 6882 from glycerol (GCP-1) and crude glycerol (GCP-2). Chemical characteristics results showed GCP-1 and GCP-2 were similar polysaccharides, but the molecular weights of GCP-1 and GCP-2 were 5.340 × 10⁴ Da and 3.105 × 10⁴ Da, respectively. Viabilities of A549 cells after treatment with GCP-1 and GCP-2 were 49% and 39% compared to the control group. Meanwhile, flow cytometry results indicated that GCP-1 and GCP-2 could induce 17.79% and 24...
December 11, 2018: Biomolecules
Huaming Xu, Nian Yang, Zhenqiang Zhang, Chunling Niu, Wensheng Yang, Junying Song, Junxia Zhang, Xiaohui Chen, Yaquan Jia, Yufang Miao
OBJECTIVES: To measure the expression of matrix metalloproteinase (MMP)-2, tissue inhibitor of matrix metalloproteinase inhibitor (TIMP)-2, and CD147 in mice with chronic liver injury induced by carbon tetrachloride after treatment with the traditional Chinese medicine (TCM) "Compound T11". METHOD: Sixty male ICR mice were divided randomly into 6 groups of 10: control (C), model (M), low-dose treatment (LT; 50 mg/mL of Compound T11), medium-dose treatment (MT, 100 mg/mL), high-dose treatment (HT, 150 mg/mL), and positive drug treatment (YT, 67...
December 13, 2018: Pharmacology
Qi Cheng, Chen Li, Cheng-Fang Yang, Yu-Juan Zhong, Dan Wu, Lin Shi, Li Chen, Yong-Wen Li, Li Li
Liver fibrosis is a pathological wound-healing response caused by chronic liver damage due to a virus, autoimmune disorder, or drugs. Hepatic stellate cells (HSCs) play an essential role in the pathogenesis of liver fibrosis. Methyl ferulic acid (MFA), a biologically active monomer, has a protective effect on liver injury. However, the effects and roles of MFA in liver fibrosis remain unknown. The purpose of the current study was to investigate the effect of MFA on hepatic fibrosis and the underlying mechanisms...
December 10, 2018: Chemico-biological Interactions
Zheng Liu, Fang Shi, Jun-Xia Liu, Chang-Lan Gao, Meng-Miao Pei, Jing Li, Pei-Xiu Li
The aim of the present study was investigate the association of the severity of emphysema of patients with chronic obstructive pulmonary disease (COPD) with airway inflammation and the COPD-specific comorbidity test (COTE) index. A total of 94 patients with COPD were divided into four groups according to the severity of their emphysema; in each patient, comorbidities were recorded and inflammatory biomarkers, including MMP-9 and TIMP-1 were determined in circulating blood. The unbalanced proportion of MMP-9 and its inhibitor, TIMP-1, led to the airway inflammation and lung remodeling in the patients with COPD...
December 2018: Experimental and Therapeutic Medicine
Sevki Peduk, Mursit Dincer, Cihad Tatar, Bahri Ozer, Ahmet Kocakusak, Gamze Citlak, Muzaffer Akinci
BACKGROUND: Gastric cancer is the 3rd most common cause of death in men and the 5th common in women worldwide. Today, surgery is the only curative therapy. Currently available advanced imaging modalities can predict R0 resection in most patients, but it can only be detected with certainty in the perioperative period. AIM: To determine the role of serum CK18, MMP9, TIMP1 levels in predicting R0 resection in patients with gastric cancer. METHODS: Fifty consecutive patients scheduled for curative surgery with gastric adenocarcinoma diagnosed between 2013-2015 were included...
December 6, 2018: Arquivos Brasileiros de Cirurgia Digestiva: ABCD, Brazilian Archives of Digestive Surgery
Zhen-Wei Ge, Bao-Cai Wang, Jun-Long Hu, Jun-Jie Sun, Sheng Wang, Xian-Jie Chen, Shu-Ping Meng, Lin Liu, Zhao-Yun Cheng
Cardiac rupture and ventricular remodeling are recognized as the severe complications and major risk factors of acute myocardial infarction (AMI). This study aims to evaluate the regulatory roles of interleukin-1 receptor-associated kinase 3 (IRAK3) and nuclear factor-κB (NF-κB) signaling pathway in cardiac rupture and ventricular remodeling. Microarray analysis was performed to screen AMI-related differentially expressed genes and IRAK3 was identified. The models of AMI were established in male C57BL/6 mice to investigate the functional role of IRAK3...
December 7, 2018: Journal of Cellular Physiology
Rashidi Springall-Del Villar, Jorge Arturo Ortega-Hernández, Luis Fernando Pérez, Fausto Sánchez-Muñoz, Julio César Arana Martínez, Héctor González-Pacheco, Rafael Bojalil
Background: Inflammation can be grouped into three phases: proinflammatory, anti-inflammatory, and resolution. The latter, mainly attributed to lipid mediators, is the most recently described, and has been studied little in coronary ischemic diseases. Objective: To evaluate 1) if acute coronary syndromes (ACS) manifest different circulating levels of resolution mediators compared with stable angina (SA); 2) if their concentrations are related to those of pro and anti-inflammatory mediators; and 3) if such concentrations are associated with the severity of the disease and the damage produced...
2018: Gaceta Médica de México
R K Kasimanickam, V R Kasimanickam, A Arangasamy, J P Kastelic
Sperm are highly specialized compartmentalized cells, with unique compositional, morphological and functional properties, including a plasma membrane that undergoes dynamic protein remodeling and surface modifications. Seminal plasma is a highly complex biological fluid containing proteins, amino acids, enzymes, fructose and other carbohydrates, lipids, major minerals and trace elements. Seminal plasma proteins are involved in regulation of osmotic pressure and pH of seminal plasma, transport of ions, lipid and hormones...
November 28, 2018: Theriogenology
David G Guzzardi, Guoqi Teng, Sean Kang, Patrick J Geeraert, Simranjit S Pattar, Daniyil A Svystonyuk, Darrell D Belke, Paul W M Fedak
OBJECTIVES: Fluoroquinolone (FQ) antibiotics are associated with adverse aortic clinical events. We assessed human aortic myofibroblast-mediated extracellular matrix (ECM) dysregulation as a possible cellular mechanism underlying FQ-associated aortopathy. METHODS: Human aortic myofibroblasts were isolated from patients with aortopathy undergoing elective ascending aortic resection (N = 9). The capacity for extracellular matrix degradation in cells exposed to FQ was assessed by multiplex analysis of secreted matrix metalloproteinases relative to tissue inhibitors of matrix metalloproteinases (TIMPs)...
September 21, 2018: Journal of Thoracic and Cardiovascular Surgery
Thomas B Piper, Lars N Jørgensen, Jesper Olsen, Knud T Nielsen, Gerard Davis, Julia S Johansen, Ib Jarle Christensen, Hans J Nielsen
BACKGROUND: Blood-based, cancer-associated biomarkers may detect subjects at risk of having neoplastic diseases. The aim of the present study was to evaluate whether elevated serological protein biomarker levels may identify adenoma patients, who are at increased risk of being diagnosed with subsequent primary malignancy. METHODS: Levels of CEA, CA19-9, TIMP-1 and YKL-40 were determined in blood samples collected prior to diagnostic bowel endoscopy due to symptoms of colorectal neoplasia...
December 7, 2018: Acta Oncologica
Ni Deng, Lili Xie, Yongwei Li, Haishu Lin, Renhui Luo
To investigate the effect of oxymatrine on periodontitis in rats and related mechanism. Methods: Ninety SD rats were divided into control, model, 10, 20 and 40 mg/kg oxymatrine and tinidazole groups. The periodontitis model was established in later 5 groups. The 10, 20 and 40 mg/kg oxymatrine groups were intragastrically administrated with 10, 20 and 40 mg/kg oxymatrine, respectively. The tinidazole group was intragastrically administrated with 100 mg/kg tinidazole. The treatment duration was 4 weeks. The tooth mobility, gingival and plaque indexes, serum inflammatory factor levels and gingival tissue matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase (TIMP) protein levels were detected...
November 2018: Acta Cirúrgica Brasileira
Shiva Roshankhah, Kamran Mansouri, Mitra Bakhtiari, Mohammad Reza Salahshoor, Rezvan Asgari
Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) involve in the degradation of the extracellular matrix (ECM) that imbalances their activity and may lead to various diseases. The present study aims to evaluate the association between MMP9-1562C/T and TIMP2-418G/C variants and synergistic effects of both variants on male infertility in an Iranian population. We analyzed these polymorphisms in 101 infertile men and 106 fertile men as a control group using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique...
December 4, 2018: Molecular Biology Reports
Xuan Qiu, Xiaobing Zhou, Yufa Miao, Bo Li
The kidney is one of the major target organs for drug-induced toxicity. During drug development, the traditional markers of nephrotoxicity indicate only severe and late damage, which leads to high costs. The new biomarkers are needed for a more sensitive and reliable evaluation of nephrotoxicity, especially for the regulatory accepted and validated in vitro model. We developed an in vitro model based on the HK-2 cell using the biomarkers of nephrotoxicity as endpoints for the evaluation of nephrotoxicity. The predictive performance of the biomarkers including LDH, GGT, KIM-1, clusterin, CysC, NGAL, TIMP-1, GSTπ and osteopontin was evaluated with 22 well characterized compounds...
November 1, 2018: Toxicology Research
Hélène Clabault, Marie Cohen, Cathy Vaillancourt, J Thomas Sanderson
INTRODUCTION: Between 2 and 10% of pregnant women are treated with selective serotonin-reuptake inhibitors (SSRIs) for depression. The extravillous trophoblasts (evTBs), which migrate and invade maternal tissues, are crucial for embryo implantation and remodeling of maternal spiral arteries. Poor migration/invasion of evTBs can cause serious pregnancy complications, yet the effects of SSRIs on these processes has never been studied. To determine the effects of five SSRIs (fluoxetine, norfluoxetine, citalopram, sertraline and venlafaxine) on migration/invasion, we used JEG-3 and HIPEC cells as evTB models...
December 2018: Placenta
T Rakmanee, E Calciolari, I Olsen, U Darbar, G S Griffiths, A Petrie, Nikolaos Donos
OBJECTIVES: To describe changes in growth factor mediators in the gingival crevicular fluid (GCF) of patients with aggressive periodontitis (AgP) undergoing regenerative (GTR) and access flap (AF) surgery. MATERIALS AND METHODS: This was a 12-month, single-blind, split-mouth RCT involving 18 AgP patients with a bilateral intrabony defect which was treated with GTR or AF. GCF was collected prior to surgery and at subsequent follow-up visits from 3 days to 12 months post-operatively, and the levels of angiopoietin-1 (Ang-1), vascular-endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), bone morphogenetic protein-2 (BMP-2), osteoprotegerin (OPG), tissue inhibitor of metalloproteinase 1 (TIMP-1), keratinocyte growth factor (KGF) and platelet-derived growth factor-AB (PDGF-AB) were measured...
November 29, 2018: Clinical Oral Investigations
Xing Ke, Lisong Shen
Epithelial ovarian cancer (EOC) is one of the major malignant cancers with high rates of early metastasis in which regulatory T cells (Tregs) play an important role. Tregs suppress immune responses and promote the development of tumours in patients with EOC. However, the underlying mechanisms remain unclear. In this study, we found higher levels of CD4+ CD25high CD127low Tregs in patients with EOC than in patients with benign ovarian tumours and healthy donors. The immune inhibitory effect of Tregs functions by maintaining high levels of immunosuppressive cytokines in EOC...
November 28, 2018: Scandinavian Journal of Immunology
Verônica Cristina Moraes Brandão, Juliana Meola, Sergio Britto Garcia, Francisco José Candido-Dos-Reis, Omero Benedicto Poli-Neto, Antonio Alberto Nogueira, Julio Cesar Rosa-E-Silva
OBJECTIVE:  To characterize the patterns of cell differentiation, proliferation, and tissue invasion in eutopic and ectopic endometrium of rabbits with induced endometriotic lesions via a well- known experimental model, 4 and 8 weeks after the endometrial implantation procedure. METHODS:  Twenty-nine female New Zealand rabbits underwent laparotomy for endometriosis induction through the resection of one uterine horn, isolation of the endometrium, and fixation of tissue segment to the pelvic peritoneum...
November 2018: Revista Brasileira de Ginecologia e Obstetrícia
Vladimir Mulens-Arias, Alice Balfourier, Alba Nicolás-Boluda, Florent Carn, Florence Gazeau
Gold nanoparticles (AuNP) have been thoroughly studied as multifunctional theranosis agents for cell imaging and cancer therapy as well as sensors due to their tunable physical and chemical properties. Although AuNP have proved to be safe in a wide concentration range, yet other important biological effects can arise in the sublethal window of treatment. This is especially pivotal to understand how AuNP can affect cell biology when labeling steps are needed for cell tracking in vivo, as nanoparticle loading can affect cell migratory/invasion ability, a function mediated by filamentous actin-rich nanometric structures collectively called adhesomes...
November 28, 2018: Biomaterials Science
Xiaodong Xie, Xiuming Zhang, Jun Chen, Xun Tang, Meiqin Wang, Lei Zhang, Zhen Guo, Wenrong Shen
Fe3O4-magnetic liposome (MLP) can deliver drugs to target tissues and can increase drug efficacy. The present study aimed to investigate the effects of solamargine (SM) and Fe3O4-SM in pancreatic cancer (PC). Cell viability was detected using a Cell Counting kit‑8 assay. Apoptosis and cell cycle progression was tested using a flow cytometry assay. A scratch assay was used to examine cell metastasis. Quantitative polymerase chain reaction, western blot analysis or immunohistochemical analysis were performed to determine the expression of target factors...
November 19, 2018: International Journal of Oncology
Ying Wang, Jia-Ning Chen, LinBin Yang, Jiaqian Li, Wei Wu, Min Huang, Ling Lin, Shicheng Su
PURPOSE: The different prognostic values of tumor-infiltrating neutrophils (TINs) in different tissue compartments are unknown. In this study, we investigated their different prognostic roles and the underlying mechanism. EXPERIMENTAL DESIGN: We evaluated CD66b+ neutrophils in primary tumors from 341 breast cancer patients from Sun Yat-Sen Memorial Hospital by immunohistochemistry. The association between stromal and parenchymal neutrophil counts and clinical outcomes was assessed in training set (170 samples), validated in internal validation set (171 samples), and further confirmed in external validation set (105 samples)...
November 27, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
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