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fibroblast coculture

Sahar Biglari, Thi Y L Le, Richard P Tan, Steven G Wise, Alessandro Zambon, Gaia Codolo, Marina De Bernard, Majid Warkiani, Aaron Schindeler, Sina Naficy, Peter Valtchev, Ali Khademhosseini, Fariba Dehghani
Considerable progress has been made in the field of microfluidics to develop complex systems for modeling human skin and dermal wound healing processes. While microfluidic models have attempted to integrate multiple cell types and/or 3D culture systems, to date they have lacked some elements needed to fully represent dermal wound healing. This paper describes a cost-effective, multicellular microfluidic system that mimics the paracrine component of early inflammation close to normal wound healing. Collagen and Matrigel are tested as materials for coating and adhesion of dermal fibroblasts and human umbilical vein endothelial cells (HUVECs)...
December 4, 2018: Advanced Healthcare Materials
Heide-Katharina Bauer, Daniela Flesch, Stefan Walenta, Ronald E Unger, Roxana Schwab, Sandra Nezi-Cahn, Annette Hasenburg, Martin Heller, Walburgis Brenner
BACKGROUND: Our aim was to establish a reliable protocol for the isolation of primary human oral mucosa epithelial cells that ensured a successful, efficient and reproducible cell culture for the utilization in subsequent experiments and for tissue engineering purposes. Therefore, we analyzed and evaluated various isolation routines and cultivation protocols. Furthermore, we investigated the functionality of isolated epithelial cells for tissue engineering approaches in an in vitro mucosa equivalent cell culture model system...
December 1, 2018: Tissue Engineering. Part C, Methods
Shuang Chen, Andreas Giannakou, Sarah Wyman, Janet Gruzas, Jonathon Golas, Wenyan Zhong, Christine Loreth, Latha Sridharan, Ting-Ting Yamin, Marc Damelin, Kenneth G Geles
Tumorigenesis depends on intricate interactions between genetically altered tumor cells and their surrounding microenvironment. While oncogenic drivers in lung squamous carcinoma (LUSC) have been described, the role of stroma in modulating tissue architecture, particularly cell polarity, remains unclear. Here, we report the establishment of a 3D coculture system of LUSC epithelial cells with cancer-associated fibroblasts (CAFs) and extracellular matrix that together capture key components of the tumor microenvironment (TME)...
November 28, 2018: Proceedings of the National Academy of Sciences of the United States of America
Yuan Liu, Lu Gao, Xiaoyan Zhao, Sen Guo, Yuzhou Liu, Ran Li, Cui Liang, Ling Li, Jianzeng Dong, Lina Li, Haibo Yang
Saikosaponin A (SSA) is a triterpenoid saponin with many pharmacological activities, including anti-inflammatory and antioxidant effects. The effect of SSA on cardiac remodeling and fibrosis, however, remains unclear. Aortic banding surgery was used to establish a mouse cardiac remodeling and fibrosis model. Mice were subjected to an intraperitoneal (i.p.) injection of SSA (5 mg/kg/d or 40 mg/kg/d) 2 weeks after surgery for 28 days. As a result, SSA had limited effect on cardiac hypertrophy but decreased cardiac fibrosis remarkably...
2018: International Journal of Biological Sciences
Ton Schoenmaker, Fenne Wouters, Dimitra Micha, Tim Forouzanfar, Coen Netelenbos, E Marelise W Eekhoff, Nathalie Bravenboer, Teun J de Vries
Fibrodysplasia ossificans progressiva (FOP) is a genetic disease characterized by heterotopic ossification (HO). The disease is caused by a mutation in the activin receptor type 1 (ACVR1) gene that enhances this receptor's responsiveness to Activin-A. Binding of Activin-A to the mutated ACVR1 receptor induces osteogenic differentiation. Whether Activin-A also affects osteoclast formation in FOP is not known. Therefore we investigated its effect on the osteoclastogenesis-inducing potential of periodontal ligament fibroblasts (PLF) from teeth of healthy controls and patients with FOP...
November 11, 2018: Journal of Cellular Physiology
Ekaterini Kefalakes, Sebastian Böselt, Anastasia Sarikidi, Miren Ettcheto, Franziska Bursch, Maximilian Naujock, Nancy Stanslowsky, Martin Schmuck, Marta Barenys, Florian Wegner, Claudia Grothe, Susanne Petri
We have previously shown that knockout of fibroblast growth factor-2 (FGF-2) and potential compensatory effects of other growth factors result in amelioration of disease symptoms in a transgenic mouse model of amyotrophic lateral sclerosis (ALS). ALS is a rapidly progressive neurological disorder leading to degeneration of cortical, brain stem, and spinal motor neurons followed by subsequent denervation and muscle wasting. Mutations in the superoxide dismutase 1 (SOD1) gene are responsible for approximately 20% of familial ALS cases and SOD1 mutant mice still are among the models best mimicking clinical and neuropathological characteristics of ALS...
October 28, 2018: Journal of Cellular Physiology
Serena P H Mao, Minji Park, Ramon M Cabrera, John R Christin, George S Karagiannis, Maja H Oktay, Dietmar M W Zaiss, Scott I Abrams, Wenjun Guo, John S Condeelis, Paraic A Kenny, Jeffrey E Segall
BACKGROUND: Amphiregulin (AREG), a ligand of the epidermal growth factor receptor, is not only essential for proper mammary ductal development, but also associated with breast cancer proliferation and growth. In the absence of AREG, mammary ductal growth is stunted and fails to expand. Furthermore, suppression of AREG expression in estrogen receptor-positive breast tumor cells inhibits in-vitro and in-vivo growth. METHODS: We crossed AREG-null (AREG-/- ) mice with the murine luminal B breast cancer model, MMTV-PyMT (PyMT), to generate spontaneous breast tumors that lack AREG (AREG-/- PyMT)...
October 26, 2018: Breast Cancer Research: BCR
Simona L Schlereth, Marcus Karlstetter, Deniz Hos, Mario Matthaei, Claus Cursiefen, Ludwig M Heindl
PURPOSE: Avascular tissues can be used to identify antilymph- or antihemangiogenic factors. The human sclera -the outer covering layer of the eye, lacks lymphatic vessels and contains only a superficial network of blood vessels and was used here to identify endogenous antiangiogenic factors. METHODS: Expression levels of a panel of 96 known pro- and antiangiogenic factors were analyzed in twelve scleral or conjunctival control samples from normal human donors using real-time PCR...
October 25, 2018: Current Eye Research
Shilpaa Mukundan, Kriti Sharma, Kim Honselmann, Amy Singleton, Andrew Liss, Biju Parekkadan
Pancreatic cancer is one of the most aggressive cancers with a 5-year patient survival rate of 8.2% and limited availability of therapeutic agents to target metastatic disease. Pancreatic cancer is characterized by a dense stromal cell population with unknown contribution to the progression or suppression of tumor growth. In this study, we describe a microengineered tumor stromal assay of patient-derived pancreatic cancer cells to study the heterotypic interactions of patient pancreatic cancer cells with different types of stromal fibroblasts under basal and drug-treated conditions...
January 1, 2018: Technology in Cancer Research & Treatment
Menglin Fan, Yanan Li, Chunhua Yao, Xiufeng Liu, Xuming Liu, Jihua Liu
Imbalance of Treg/Th17 and chronic synovitis characterized by the recruitment and infiltration of inflammatory cells are the typical features of rheumatoid arthritis (RA). IL-6 promotes the differentiation and function of Th17 cells, which contributes to the imbalance of Treg/Th17 and aggravates lymphocytic infiltration in joints. DC32, a dihydroartemisinin derivative, was found to have anti-inflammatory and immunosuppressive activities in previous study. The aim of this study is to evaluate the effects and mechanisms of DC32 in immunodeficiency and inflammatory infiltration of RA...
December 2018: International Immunopharmacology
Ryuichiro Kimura, Azusa Yoneshige, Man Hagiyama, Tomoyuki Otani, Takao Inoue, Naoki Shiraishi, Kazuyoshi Yanagihara, Tomohiko Wakayama, Akihiko Ito
AIMS: To determine cellular distribution of cell adhesion molecule 1 (CADM1), an immunoglobulin superfamily member, in the human oxyntic gastric mucosa, and to explore possible involvement in the development and peritoneal dissemination of signet ring cell (SRC) gastric carcinoma, which often develops in the oxyntic mucosa. MAIN METHODS: Immunohistochemistry and double immunofluorescence were conducted on surgical specimens of normal and SRC-bearing stomachs and peritoneal metastatic foci of SRCs...
October 9, 2018: Life Sciences
Ching-Wen Tsai, Jyh-Horng Wang, Tai-Horng Young
An ideal in vitro drug screening model is important for the drug development. In addition to monoculture systems, 3 dimensional (3D) coculture systems are extensively used to simulate the in vivo tumor microenvironment as cell-cell and cell-extracellular matrix interactions within the tumor tissues can be mimicked. In this study, in vitro 3D suspension coculture multicellular spheroids with core/shell cell distribution were developed on chitosan-coated surfaces. Based on the characteristic of chitosan inhibiting cell adhesion, SW620 (colon cancer cell line), 3A6 (mesenchymal stem-like cell line) and Hs68 (foreskin fibroblast line) cells could aggregate to form 3D coculture spheroids with intimate cell contacts...
October 12, 2018: Artificial Cells, Nanomedicine, and Biotechnology
John Nowlin, Mehzubh A Bismi, Baptiste Delpech, Patrick Dumas, Yingge Zhou, George Z Tan
Tendon injuries can be difficult to heal and have high rates of relapse due to stress concentrations caused by scar formation and the sutures used in surgical repair. Regeneration of the tendon/ligament-to-bone interface is critical to provide functional graft integration after injury. The objective of this study is to recreate the tendon-to-bone interface using a gradient scaffold which is fabricated by a one-station electrospinning process. Two cell phenotypes were grown on a poly- ε -caprolactone nanofiber scaffold which possesses a gradual transition from random to aligned nanofiber patterns...
January 2018: Nanobiomedicine
Na Li, Hanna Sanyour, Tyler Remund, Patrick Kelly, Zhongkui Hong
Construction of an artificial vascular graft is widely considered a promising strategy in vascular tissue engineering. However, limited sources of functional vascular smooth muscle cells (VSMCs) remain a major obstacle in vascular tissue engineering. In this study, we innovatively developed an approach to obtain functional VSMCs by onsite differentiating human bone marrow-derived mesenchymal stem cells (MSCs) directed by decellularized extracellular matrix (ECM) and fibroblasts. The resulting cells and ECM-cells constructs were characterized by real time RT-PCR, immunofluorescence staining, cell contractile functions, and migration capacity...
December 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Masaki Suzuki, Takehiko Yokobori, Navchaa Gombodorj, Masakazu Yashiro, Andrei Turtoi, Tadashi Handa, Kyoichi Ogata, Tetsunari Oyama, Ken Shirabe, Hiroyuki Kuwano
BACKGROUND AND OBJECTIVES: Transforming growth factor β-induced (TGFBI) protein is a secreted extracellular matrix protein with conflicting roles in cancer, acting as a tumour suppressor and a promoter, which appears to be tissue specific. The role of TGFBI in gastric cancer (GC) remains unclear, which we aimed to investigate using the clinical samples as well as an in vitro coculture model of GC. METHODS: The clinical significance of TGFBI was assessed in 208 GC samples using immunohistochemistry...
November 2018: Journal of Surgical Oncology
Lindsay J Celada, Jonathan A Kropski, Jose D Herazo-Maya, Weifeng Luo, Amy Creecy, Andrew T Abad, Ozioma S Chioma, Grace Lee, Natalie E Hassell, Guzel I Shaginurova, Yufen Wang, Joyce E Johnson, Amy Kerrigan, Wendi R Mason, Robert P Baughman, Gregory D Ayers, Gordon R Bernard, Daniel A Culver, Courtney G Montgomery, Toby M Maher, Philip L Molyneaux, Imre Noth, Steven E Mutsaers, Cecilia M Prele, R S Peebles, Dawn C Newcomb, Naftali Kaminski, Timothy S Blackwell, Luc Van Kaer, Wonder P Drake
Pulmonary fibrosis is a progressive inflammatory disease with high mortality and limited therapeutic options. Previous genetic and immunologic investigations suggest common intersections between idiopathic pulmonary fibrosis (IPF), sarcoidosis, and murine models of pulmonary fibrosis. To identify immune responses that precede collagen deposition, we conducted molecular, immunohistochemical, and flow cytometric analysis of human and murine specimens. Immunohistochemistry revealed programmed cell death-1 (PD-1) up-regulation on IPF lymphocytes...
September 26, 2018: Science Translational Medicine
Ben Antebi, Kerfoot P Walker, Arezoo Mohammadipoor, Luis A Rodriguez, Robbie K Montgomery, Andriy I Batchinsky, Leopoldo C Cancio
BACKGROUND: It is known that, following a physiological insult, bone marrow-derived mesenchymal stem cells (MSCs) mobilize and home to the site of injury. However, the effect of injury on the function of endogenous MSCs is unknown. In this study, MSCs harvested from the bone marrow of swine with or without acute respiratory distress syndrome (ARDS) were assessed for their characteristics and therapeutic function. METHODS: MSCs were harvested from three groups of anesthetized and mechanically ventilated swine (n = 3 in each group): 1) no ARDS ('Uninjured' group); 2) ARDS induced via smoke inhalation and 40% burn and treated with inhaled epinephrine ('Injured Treated' group); and 3) ARDS without treatment ('Injured Untreated' group)...
September 26, 2018: Stem Cell Research & Therapy
Shira Landau, Shahar Ben-Shaul, Shulamit Levenberg
Endothelial cells form the interior layer of blood vessels and, as such, are constantly exposed to shear stress and mechanical strain. While the impact of shear stress on angiogenesis is widely studied, the role of mechanical strain is less understood. To this end, endothelial cells and fibroblasts are cocultured under oscillatory strain to create a vessel network. The two cell types show distinctly different sensitivities to the mechanical stimulation. The fibroblasts, sense the stress directly, and respond by increased alignment, proliferation, differentiation, and migration, facilitated by YAP translocation into the nucleus...
September 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
Yanling Liao, Larisa Ivanova, Hongwen Zhu, Trevor Plumer, Carl Hamby, Brinda Mehta, Annie Gevertz, Angela M Christiano, John A McGrath, Mitchell S Cairo
Recessive dystrophic epidermolysis bullosa (RDEB) is a severe skin fragility disorder caused by mutations in the Col7a1 gene. Patients with RDEB suffer from recurrent erosions in skin and mucous membranes and have a high risk for developing cutaneous squamous cell carcinoma (cSCCs). TGFβ signaling has been associated with fibrosis and malignancy in RDEB. In this study, the activation of TGFβ signaling was demonstrated in col7a1-/- mice as early as a week after birth starting in the interdigital folds of the paws, accompanied by increased deposition of collagen fibrils and elevated dermal expression of matrix metalloproteinase (MMP)-9 and MMP-13...
September 24, 2018: Stem Cells
Gracjana Krzysiek-Maczka, Aneta Targosz, Urszula Szczyrk, Malgorzata Strzałka, Zbigniew Sliwowski, Tomasz Brzozowski, Jarosław Czyz, Agata Ptak-Belowska
BACKGROUND: Major human gastrointestinal pathogen Helicobacter pylori (H. pylori) colonizes the gastric mucosa causing inflammation and severe complications including cancer, but the involvement of fibroblasts in the pathogenesis of these disorders in H. pylori-infected stomach has been little studied. Normal stroma contains few fibroblasts, especially myofibroblasts. Their number rapidly increases in the reactive stroma surrounding inflammatory region and neoplastic tissue; however, the interaction between H...
September 23, 2018: Helicobacter
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