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fibroblast coculture

Ryuichiro Kimura, Azusa Yoneshige, Man Hagiyama, Tomoyuki Otani, Takao Inoue, Naoki Shiraishi, Kazuyoshi Yanagihara, Tomohiko Wakayama, Akihiko Ito
AIMS: To determine cellular distribution of cell adhesion molecule 1 (CADM1), an immunoglobulin superfamily member, in the human oxyntic gastric mucosa, and to explore possible involvement in the development and peritoneal dissemination of signet ring cell (SRC) gastric carcinoma, which often develops in the oxyntic mucosa. MAIN METHODS: Immunohistochemistry and double immunofluorescence were conducted on surgical specimens of normal and SRC-bearing stomachs and peritoneal metastatic foci of SRCs...
October 9, 2018: Life Sciences
Ching-Wen Tsai, Jyh-Horng Wang, Tai-Horng Young
An ideal in vitro drug screening model is important for the drug development. In addition to monoculture systems, 3 dimensional (3D) coculture systems are extensively used to simulate the in vivo tumor microenvironment as cell-cell and cell-extracellular matrix interactions within the tumor tissues can be mimicked. In this study, in vitro 3D suspension coculture multicellular spheroids with core/shell cell distribution were developed on chitosan-coated surfaces. Based on the characteristic of chitosan inhibiting cell adhesion, SW620 (colon cancer cell line), 3A6 (mesenchymal stem-like cell line) and Hs68 (foreskin fibroblast line) cells could aggregate to form 3D coculture spheroids with intimate cell contacts...
October 12, 2018: Artificial Cells, Nanomedicine, and Biotechnology
John Nowlin, Mehzubh A Bismi, Baptiste Delpech, Patrick Dumas, Yingge Zhou, George Z Tan
Tendon injuries can be difficult to heal and have high rates of relapse due to stress concentrations caused by scar formation and the sutures used in surgical repair. Regeneration of the tendon/ligament-to-bone interface is critical to provide functional graft integration after injury. The objective of this study is to recreate the tendon-to-bone interface using a gradient scaffold which is fabricated by a one-station electrospinning process. Two cell phenotypes were grown on a poly- ε -caprolactone nanofiber scaffold which possesses a gradual transition from random to aligned nanofiber patterns...
January 2018: Nanobiomedicine
Na Li, Hanna Sanyour, Tyler Remund, Patrick Kelly, Zhongkui Hong
Construction of an artificial vascular graft is widely considered a promising strategy in vascular tissue engineering. However, limited sources of functional vascular smooth muscle cells (VSMCs) remain a major obstacle in vascular tissue engineering. In this study, we innovatively developed an approach to obtain functional VSMCs by onsite differentiating human bone marrow-derived mesenchymal stem cells (MSCs) directed by decellularized extracellular matrix (ECM) and fibroblasts. The resulting cells and ECM-cells constructs were characterized by real time RT-PCR, immunofluorescence staining, cell contractile functions, and migration capacity...
December 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Masaki Suzuki, Takehiko Yokobori, Navchaa Gombodorj, Masakazu Yashiro, Andrei Turtoi, Tadashi Handa, Kyoichi Ogata, Tetsunari Oyama, Ken Shirabe, Hiroyuki Kuwano
BACKGROUND AND OBJECTIVES: Transforming growth factor β-induced (TGFBI) protein is a secreted extracellular matrix protein with conflicting roles in cancer, acting as a tumour suppressor and a promoter, which appears to be tissue specific. The role of TGFBI in gastric cancer (GC) remains unclear, which we aimed to investigate using the clinical samples as well as an in vitro coculture model of GC. METHODS: The clinical significance of TGFBI was assessed in 208 GC samples using immunohistochemistry...
September 9, 2018: Journal of Surgical Oncology
Lindsay J Celada, Jonathan A Kropski, Jose D Herazo-Maya, Weifeng Luo, Amy Creecy, Andrew T Abad, Ozioma S Chioma, Grace Lee, Natalie E Hassell, Guzel I Shaginurova, Yufen Wang, Joyce E Johnson, Amy Kerrigan, Wendi R Mason, Robert P Baughman, Gregory D Ayers, Gordon R Bernard, Daniel A Culver, Courtney G Montgomery, Toby M Maher, Philip L Molyneaux, Imre Noth, Steven E Mutsaers, Cecilia M Prele, R Stokes Peebles, Dawn C Newcomb, Naftali Kaminski, Timothy S Blackwell, Luc Van Kaer, Wonder P Drake
Pulmonary fibrosis is a progressive inflammatory disease with high mortality and limited therapeutic options. Previous genetic and immunologic investigations suggest common intersections between idiopathic pulmonary fibrosis (IPF), sarcoidosis, and murine models of pulmonary fibrosis. To identify immune responses that precede collagen deposition, we conducted molecular, immunohistochemical, and flow cytometric analysis of human and murine specimens. Immunohistochemistry revealed programmed cell death-1 (PD-1) up-regulation on IPF lymphocytes...
September 26, 2018: Science Translational Medicine
Ben Antebi, Kerfoot P Walker, Arezoo Mohammadipoor, Luis A Rodriguez, Robbie K Montgomery, Andriy I Batchinsky, Leopoldo C Cancio
BACKGROUND: It is known that, following a physiological insult, bone marrow-derived mesenchymal stem cells (MSCs) mobilize and home to the site of injury. However, the effect of injury on the function of endogenous MSCs is unknown. In this study, MSCs harvested from the bone marrow of swine with or without acute respiratory distress syndrome (ARDS) were assessed for their characteristics and therapeutic function. METHODS: MSCs were harvested from three groups of anesthetized and mechanically ventilated swine (n = 3 in each group): 1) no ARDS ('Uninjured' group); 2) ARDS induced via smoke inhalation and 40% burn and treated with inhaled epinephrine ('Injured Treated' group); and 3) ARDS without treatment ('Injured Untreated' group)...
September 26, 2018: Stem Cell Research & Therapy
Shira Landau, Shahar Ben-Shaul, Shulamit Levenberg
Endothelial cells form the interior layer of blood vessels and, as such, are constantly exposed to shear stress and mechanical strain. While the impact of shear stress on angiogenesis is widely studied, the role of mechanical strain is less understood. To this end, endothelial cells and fibroblasts are cocultured under oscillatory strain to create a vessel network. The two cell types show distinctly different sensitivities to the mechanical stimulation. The fibroblasts, sense the stress directly, and respond by increased alignment, proliferation, differentiation, and migration, facilitated by YAP translocation into the nucleus...
September 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
Yanling Liao, Larisa Ivanova, Hongwen Zhu, Trevor Plumer, Carl Hamby, Brinda Mehta, Annie Gevertz, Angela M Christiano, John A McGrath, Mitchell S Cairo
Recessive dystrophic epidermolysis bullosa (RDEB) is a severe skin fragility disorder caused by mutations in the Col7a1 gene. Patients with RDEB suffer from recurrent erosions in skin and mucous membranes and have a high risk for developing cutaneous squamous cell carcinoma (cSCCs). TGFβ signaling has been associated with fibrosis and malignancy in RDEB. In this study, the activation of TGFβ signaling was demonstrated in col7a1-/- mice as early as a week after birth starting in the interdigital folds of the paws, accompanied by increased deposition of collagen fibrils and elevated dermal expression of matrix metalloproteinase (MMP)-9 and MMP-13...
September 24, 2018: Stem Cells
Gracjana Krzysiek-Maczka, Aneta Targosz, Urszula Szczyrk, Malgorzata Strzałka, Zbigniew Sliwowski, Tomasz Brzozowski, Jarosław Czyz, Agata Ptak-Belowska
BACKGROUND: Major human gastrointestinal pathogen Helicobacter pylori (H. pylori) colonizes the gastric mucosa causing inflammation and severe complications including cancer, but the involvement of fibroblasts in the pathogenesis of these disorders in H. pylori-infected stomach has been little studied. Normal stroma contains few fibroblasts, especially myofibroblasts. Their number rapidly increases in the reactive stroma surrounding inflammatory region and neoplastic tissue; however, the interaction between H...
September 23, 2018: Helicobacter
Anita Rogic, Francois Auger, Mihaela Skobe
Studies of lymphangiogenesis and lymphatic endothelial biology in vitro require pure cultures of lymphatic endothelial cells and 3D vascular constructs, which closely resemble native human lymphatic vasculature. We describe a method for the isolation of human dermal microvascular lymphatic endothelial cells and generation of a 3D lymphatic capillary network. The lymphatic vascular construct is generated by coculturing primary lymphatic endothelial cells and fibroblasts in their native matrix, without the use of synthetic scaffolds or exogenous factors...
2018: Methods in Molecular Biology
Wendy Dankers, Claudia González-Leal, Nadine Davelaar, Patrick S Asmawidjaja, Adriana M C Mus, Johanna M W Hazes, Edgar M Colin, Erik Lubberts
BACKGROUND: Despite recent improvements in the treatment of rheumatoid arthritis (RA), an insufficient treatment response and the development of treatment resistance in many patients illustrates the need for new therapeutic strategies. Chronic synovial inflammation could be suppressed by targeting RA synovial fibroblast (RASF) activation by, for example, interleukin (IL)-17A-producing CCR6+ T helper memory (memTh) cells. Here, we modulated this interaction by combining the active vitamin D metabolite 1,25(OH)2 D3 with dexamethasone (DEX) and explored the potential therapeutic applications...
September 20, 2018: Arthritis Research & Therapy
Takuro Miyazaki, Shogo Haraguchi, Joo-Ri Kim-Kaneyama, Akira Miyazaki
The transformation of fibroblasts to myofibroblasts plays a major role in fibrogenic responses during dermal wound healing. We show a contribution of calpain systems (intracellular regulatory protease systems) in vascular endothelial cells (ECs) to myofibroblast differentiation in wound sites. Dermal wound healing experiments in mice found that calpastatin (an endogenous inhibitor of calpains) is enriched in preexisting vessels but not in newly formed capillaries. Transgenic overexpression of calpastatin in ECs delayed wound healing in mice as well as reducing the keratinocyte layer, extracellular matrix deposition, and myofibroblast accumulation in wound sites...
September 10, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Emily Chen, Bruna Benso, Dalia Seleem, Luiz Eduardo Nunes Ferreira, Silvana Pasetto, Vanessa Pardi, Ramiro Mendonça Murata
Current treatments for Candida albicans infection are limited due to the limited number of antifungal drugs available and the increase in antifungal resistance. Curcumin is used as a spice, food preservative, flavoring, and coloring agent that has been shown to have many pharmacological activities. Thus, this study evaluated the modulatory effects of curcumin on major virulence factors associated with the pathogenicity of C. albicans . The minimum inhibitory concentration (MIC) of curcumin against C. albicans (SC5314) was determined...
2018: International Journal of Dentistry
Ines Barone, Valentina Vircillo, Cinzia Giordano, Luca Gelsomino, Balázs Győrffy, Roberta Tarallo, Antonio Rinaldi, Giuseppina Bruno, Antonella Caruso, Francesco Romeo, Daniela Bonofiglio, Sebastiano Andò, Stefania Catalano
Cancer-associated Fibroblasts (CAFs), the principal components of tumor microenvironment, play multiple role in breast cancer progression. We have previously shown an oncosuppressive role of the nuclear Farnesoid X Receptor (FXR) in mammary epithelial cancer cells, here we assessed whether FXR activation may affect CAF tumor-promoting features. We showed that FXR is expressed in human CAFs isolated from four patients and treatment with the selective FXR agonist GW4064 decreased CAF migration, stress-fiber formation and contractility...
September 1, 2018: Cancer Letters
Ramamoorthi Ganesan, Mahaboobkhan Rasool
Interleukin 17 (IL-17), a proinflammatory cytokine produced by T helper (Th) 17 cells, potentially controls fibroblast-like synoviocytes (FLS)-mediated disease activity of rheumatoid arthritis (RA) via IL-17/ IL-17 receptor type A (IL-17RA)/signal transducer and activator of transcription 3 (STAT-3) signaling cascade. This has suggested that targeting IL-17 signaling could serve as an important strategy to treat FLS-mediated RA progression. Ferulic acid (FA), a key polyphenol, attenuates the development of gouty arthritis and cancer through its anti-inflammatory effects, but its therapeutic efficiency on IL-17 signaling in FLS-mediated RA pathogenesis remains unknown...
August 30, 2018: Journal of Cellular Biochemistry
Khushboo Agrawal, Viswanath Das, Natálie Táborská, Ján Gurský, Petr Džubák, Marián Hajdúch
The advanced-stage colon cancer spreads from primary tumor site to distant organs where the colon-unassociated stromal population provides a favorable niche for the growth of tumor cells. The heterocellular interactions between colon cancer cells and colon-unassociated fibroblasts at distant metastatic sites are important, yet these cell-cell interactions for therapeutic strategies for metastatic colon cancer remain underestimated. Recent studies have shown the therapeutic potential of DNA-demethylating epi-drugs 5-azacytidine (AZA) and 5-aza-2'-deoxycytidine (DAC) for the treatment of solid tumors...
2018: Stem Cells International
Ivan J Ascari, Sávio C Martins, Luiz S A Camargo, Rosalia Mendez-Otero, Jasmin
Despite the progress on development of new culture media, in vitro-produced embryos still display lower quality when compared to the in vivo-produced counterparts. Coculture has been reconsidered as an alternative to improve embryo quality. Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been extensively used as feeder layers due to their capacity to release growth factors. In the present study we investigated the effect of these feeder layers in oocyte maturation and/or embryo development under in vitro conditions...
August 25, 2018: Molecular Biology Reports
Carolyn G J Moonen, Sven T Alders, Hetty J Bontkes, Ton Schoenmaker, Elena A Nicu, Bruno G Loos, Teun J de Vries
Periodontitis, a chronic inflammatory disease of the periodontium, is characterized by osteoclast-mediated alveolar bone destruction. Gingival fibroblasts (GFs) present in the bone-lining mucosa have the capacity to activate the formation of osteoclasts, but little is known about which local immune cells (co-)mediate this process. The aim of this study was to investigate the cellular interactions of GFs with immune cells, including the contribution of GFs to osteoclast formation and their possible role in the proliferation of these immune cells...
2018: Frontiers in Immunology
Vishwaraj Sontake, Rajesh K Kasam, Debora Sinner, Thomas R Korfhagen, Geereddy B Reddy, Eric S White, Anil G Jegga, Satish K Madala
Wilms' tumor 1 (WT1) is a critical transcriptional regulator of mesothelial cells during lung development but is downregulated in postnatal stages and adult lungs. We recently showed that WT1 is upregulated in both mesothelial cells and mesenchymal cells in the pathogenesis of idiopathic pulmonary fibrosis (IPF), a fatal fibrotic lung disease. Although WT1-positive cell accumulation leading to severe fibrotic lung disease has been studied, the role of WT1 in fibroblast activation and pulmonary fibrosis remains elusive...
August 23, 2018: JCI Insight
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