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https://www.readbyqxmd.com/read/30513471/facile-fabrication-of-superporous-and-biocompatible-hydrogel-scaffolds-for-artificial-corneal-periphery
#1
Yu-Ping Lee, Hsin-Yu Liu, Po-Chun Lin, Yi-Huan Lee, Leng-Rong Yu, Chih-Chen Hsieh, Po-Jen Shih, Wen-Pin Shih, I-Jong Wang, Jia-Yush Yen, Chi-An Dai
Biocompatible and highly porous network hydrogel scaffolds were fabricated for the development of artificial cornea (AC) periphery/skirt that could be used to enhance the long-term retention of the implants. In this study, a series of hydrogel scaffolds for this application was fabricated from the photo-polymerization of a mixture of poly(ethylene glycol) (PEG)- and poloxamer (P407)-based macromer solutions in dichloromethane in which solvent-induced phase separation (SIPS) arose to form scaffolds with macroporous structure and high water content...
November 8, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30509181/preharvest-application-of-ethephon-and-postharvest-uv-b-radiation-improve-quality-traits-of-beetroot-beta-vulgaris-l-ssp-vulgaris-as-source-of-colourant
#2
Gregorio Barba-Espin, Stephan Glied-Olsen, Tsaneta Dzhanfezova, Bjarne Joernsgaard, Henrik Lütken, Renate Müller
BACKGROUND: Betanins have become excellent replacers for artificial red-purple food colourants. Red beet (Beta vulgaris L. spp. vulgaris) known as beetroot, is a rich source of betalains, which major forms are betanin (red to purple) and vulgaxanthin (yellow). Betalains and phenolic compounds are secondary metabolites, accumulation of which is often triggered by elicitors during plant stress responses. In the present study, pre-harvest applications of ethephon (an ethylene-releasing compound) and postharvest UV-B radiation were tested as elicitors of betalains and phenolic compounds in two beetroot cultivars...
December 3, 2018: BMC Plant Biology
https://www.readbyqxmd.com/read/30506149/glucose-installed-biodegradable-polymeric-micelles-for-cancer-targeted-drug-delivery-system-synthesis-characterization-and-in-vitro-evaluation
#3
Man Theerasilp, Punlop Chalermpanapun, Panya Sunintaboon, Witaya Sungkarat, Norased Nasongkla
Glucose metabolism of cancer can be used as a strategy to target cancer cells which exhibit altered glycolytic rate. The facilitated glucose transporter (Glut) plays an important role in enhancement glycolytic rate resulting in increased glucose uptake into cancer cells. 18 FGD-PET image is an example for using Glut as a targeting to diagnose the high glycolytic rate of tumor. Thus, Glut may be adapted to target cancer cells for drug delivery system. Herein, biodegradation polymeric micelles target cancer cells by Glut was fabricated...
November 30, 2018: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/30505339/measuring-ethylene-in-postharvest-biology-research-using-the-laser-based-etd-300-ethylene-detector
#4
Sunny George Gwanpua, Abdul Jabbar, Jeritah Tongonya, Sue Nicholson, Andrew R East
Background: Ability to measure ethylene is an important aspect of postharvest management, as knowledge of endogenous ethylene production is used in assessing physiological status, while response of crops to exogenous ethylene informs efforts needed to control unwanted ripening. An ethylene monitoring device with a laser-based photoacoustic detector, ETD-300, was recently developed by Sensor Sense B.V., Nijmegen, The Netherlands. In terms of performance, the ETD-300 is superior to all other current ethylene measurement devices, with a sensitivity of 0...
2018: Plant Methods
https://www.readbyqxmd.com/read/30502918/evaluation-of-protein-separations-based-on-hydrophobic-interaction-chromatography-using-polyethylene-terephthalate-capillary-channeled-polymer-c-cp-fiber-phases
#5
Lei Wang, R Kenneth Marcus
Poly(ethylene terephthalate) (polyester, PET) capillary-channeled polymer fibers are used as the stationary phase for the separation of a synthetic protein mixtures composed of cytochrome c, myoglobin, ribonuclease A, lysozyme, α-chymotrypsinogen A, α-chymotrypsin and holo-transferrin. Polyester is a useful phase for HIC, having a mixed alkyl and aromatic backbone to yield mildly hydrophobic properties, an aromatic functionality allowing for π-π interactions, and carboxylate end groups adding hydrophilicity...
November 26, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/30501023/-epsc-involved-in-the-encoding-of-exopolysaccharides-produced-by-bacillus-amyloliquefaciens-fzb42-act-to-boost-the-drought-tolerance-of-arabidopsis-thaliana
#6
Xiang Lu, Shao-Fang Liu, Liang Yue, Xia Zhao, Yu-Bao Zhang, Zhong-Kui Xie, Ruo-Yu Wang
Bacillus amyloliquefaciens FZB42 is a plant growth-promoting rhizobacteria that stimulates plant growth, and enhances resistance to pathogens and tolerance of salt stress. Instead, the mechanistic basis of drought tolerance in Arabidopsis thaliana induced by FZB42 remains unexplored. Here, we constructed an exopolysaccharide-deficient mutant epsC and determined the role of epsC in FZB42-induced drought tolerance in A. thaliana . Results showed that FZB42 significantly enhanced growth and drought tolerance of Arabidopsis by increasing the survival rate, fresh and dry shoot weights, primary root length, root dry weight, lateral root number, and total lateral root length...
November 29, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30497010/immunofunctional-photodegradable-poly-ethylene-glycol-hydrogel-surfaces-for-the-capture-and-release-of-rare-cells
#7
Paige J LeValley, Mark W Tibbitt, Ben Noren, Prathamesh Kharkar, April M Kloxin, Kristi S Anseth, Mehmet Toner, John Oakey
Circulating tumor cells (CTCs) play a central role in cancer metastasis and represent a rich source of data for cancer prognostics and therapeutic guidance. Reliable CTC recovery from whole blood therefore promises a less invasive and more sensitive approach to cancer diagnosis and progression tracking. CTCs, however, are exceedingly rare in whole blood, making their quantitative recovery challenging. Several techniques capable of isolating these rare cells have been introduced and validated, yet most suffer from low CTC purity or viability, both of which are essential to develop a clinically viable CTC isolation platform...
November 20, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30486949/preparation-and-photocatalytic-properties-of-zno-deposited-tio%C3%A2-nanotube-arrays-by-anodization
#8
Fanmin Shang, Suiyuan Chen, Jing Liang, Changsheng Liu
ZnO doped TiO₂ nanotube arrays (ZnO-TNTs) with an average diameter of 60~80 nm and an average length of 2~4 μ m were prepared on the Ti substrate by a one-step anodizing method using NH₄F/ethylene glycol as the electrolyte. The phase structure, morphology, chemical composition, photocatalytic property and mechanism of TNTs were studied by scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction, transmission electron microscopy, UV-vis diffuse reflectance spectra, photoluminescence and photocatalytic degradation of methylene blue under visible light...
April 1, 2019: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/30479928/development-of-biocompatible-ha-hydrogels-embedded-with-a-new-synthetic-peptide-promoting-cellular-migration-for-advanced-wound-care-management
#9
Sun Young Wang, Hyosuk Kim, Gijung Kwak, Hong Yeol Yoon, Sung Duk Jo, Ji Eun Lee, Daeho Cho, Ick Chan Kwon, Sun Hwa Kim
In the past few years, there have been many efforts underway to develop effective wound healing treatments for traumatic injuries. In particular, wound-healing peptides (WHPs) and peptide-grafted dressings hold great promise for novel therapeutic strategies for wound management. This study reports a topical formulation of a new synthetic WHP (REGRT, REG) embedded in a hyaluronic acid (HA)-based hydrogel dressing for the enhancement of acute excisional wound repair. The copper-free click chemistry is utilized to form biocompatible HA hydrogels by cross-linking dibenzocyclooctyl-functionalized HA with 4-arm poly(ethylene glycol) (PEG) azide...
November 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/30477300/microfluidic-manufacturing-of-sn-38-loaded-polymer-nanoparticles-with-shear-processing-control-of-drug-delivery-properties
#10
Yi-Meng Cao, Lisa K Silverman, Changhai Lu, Rebecca Hof, Jeremy E Wulff, Matthew G Moffitt
Two-phase gas-liquid microfluidic reactors provide shear processing control of SN-38-loaded polymer nanoparticles (SN-38-PNPs). We prepare SN-38-PNPs from the block copolymer poly(methyl caprolactone-co-caprolactone)-block-poly(ethylene oxides) (P(MCL-co-CL)-b-PEO) using bulk and microfluidic methods and at different drug-to-polymer loading ratios and on-chip flow rates. We show that as the microfluidic flow rate (Q) increases, encapsulation efficiency and drug loading increase and release half times increase...
November 26, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/30477265/electrospun-alginate-fibers-mixing-of-two-different-poly-ethylene-oxide-grades-to-improve-fiber-functional-properties
#11
Barbara Vigani, Silvia Rossi, Giulia Milanesi, Maria Cristina Bonferoni, Giuseppina Sandri, Giovanna Bruni, Franca Ferrari
The aim of the present work was to investigate how the molecular weight (MW) of poly(ethylene oxide) (PEO), a synthetic polymer able to improve alginate (ALG) electrospinnability, could affect ALG-based fiber morphology and mechanical properties. Two PEO grades, having different MWs (high, h-PEO, and low, l-PEO) were blended with ALG: the concentrations of both PEOs in each mixture were defined so that each h-PEO/l-PEO combination would show the same viscosity at high shear rate. Seven ALG/h-PEO/l-PEO mixtures were prepared and characterized in terms of viscoelasticity and conductivity and, for each mixture, a complex parameter rH/rL was calculated to better identify which of the two PEO grades prevails over the other in terms of exceeding the critical entanglement concentration...
November 25, 2018: Nanomaterials
https://www.readbyqxmd.com/read/30477205/enhanced-photocatalytic-performance-of-nitrogen-doped-tio%C3%A2-nanotube-arrays-using-a-simple-annealing-process
#12
Phuoc Huu Le, Le Trung Hieu, Tu-Ngoc Lam, Nguyen Thi Nhat Hang, Nguyen Van Truong, Le Thi Cam Tuyen, Pham Thanh Phong, Jihperng Leu
Nitrogen-doped TiO₂ nanotube arrays (N-TNAs) were successfully fabricated by a simple thermal annealing process in ambient N₂ gas at 450 °C for 3 h. TNAs with modified morphologies were prepared by a two-step anodization using an aqueous NH₄F/ethylene glycol solution. The N-doping concentration (0⁻9.47 at %) can be varied by controlling N₂ gas flow rates between 0 and 500 cc/min during the annealing process. Photocatalytic performance of as-prepared TNAs and N-TNAs was studied by monitoring the methylene blue degradation under visible light (λ ≥ 400 nm) illumination at 120 mW·cm-2 ...
November 24, 2018: Micromachines
https://www.readbyqxmd.com/read/30470021/measurement-of-ultrafast-combustion-process-of-premixed-ethylene-oxygen-flames-in-narrow-channel-with-digital-holographic-interferometry
#13
Teli Xi, Jianglei Di, Ying Li, Siqing Dai, Chaojie Ma, Jianlin Zhao
The premixed ethylene and oxygen flame that is burning in a narrow channel is investigated with digital holographic interferometry (DHI). Combustion in either a narrow tube or channel is quite different. This is caused by the significant effects of the boundary layer. The flame's acceleration rate will be enhanced as the tube diameter decreases. Usually, flame and shock wave propagation, which occurs during the premixed ethylene/oxygen flame combustion in the measurement area, is less than few milliseconds, so that general camera can rarely capture this fast event...
October 29, 2018: Optics Express
https://www.readbyqxmd.com/read/30453535/effect-of-formulation-on-the-binding-efficiency-and-selectivity-of-precipitation-molecularly-imprinted-polymers
#14
K Fremielle Lim, Clovia I Holdsworth
This study investigated the effect of feed formulation: the template:functional monomer (T:fM) and functional monomer:crosslinker (fM:X) ratios as well as the initiator concentration, on the binding performance and selectivity of caffeine (CAF) and theophylline (THP) imprinted polymers obtained by precipitation polymerisation in acetonitrile at 60 °C using methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) as functional monomer and crosslinker, respectively. Template incorporation, monitored by quantitative ¹H-NMR spectroscopy, ranged from 8 to 77% and was found to be more favourable at both high and low T:fM ratios, low fM:X ratio and high initiator concentration...
November 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30451358/morphological-dissection-and-cellular-and-transcriptome-characterizations-of-bamboo-pith-cavity-formation-reveal-a-pivotal-role-of-genes-related-to-programmed-cell-death
#15
Lin Guo, Xuepeng Sun, Zhongru Li, Yujun Wang, Zhangjun Fei, Chen Jiao, Jianyuan Feng, Dingfan Cui, Xingyu Feng, Yulong Ding, Chunxia Zhang, Qiang Wei
Pith cavity formation is critical for bamboo to overcome the bending force during its fast growth; however, the underlying molecular mechanisms remain largely unknown. Multiple approaches, including anatomical dissection, mathematical modelling and transcriptome profiling, were employed in this study to investigate the biology of pith cavity formation in bamboo Pseudosasa japonica. We found that the corruption of pith tissue occurred sequentially and asymmetrically from the top-center of the internode down to the bottom, which might be caused by the combined effects of asymmetrical radial and axial tensile forces during shoot-wall cell elongation and spiral growth of bamboo internodes...
November 19, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/30444361/nucleophilicity-and-electrophilicity-parameters-for-predicting-absolute-rate-constants-of-highly-asynchronous-1-3-dipolar-cycloadditions-of-aryldiazomethanes
#16
Harish Jangra, Quan Chen, Elina Fuks, Ivo Zenz, Peter Mayer, Armin R Ofial, Hendrik Zipse, Herbert Mayr
Kinetics of the reactions of aryldiazomethanes (ArCHN2 ) with benzhydrylium ions (Ar2 CH+ ) have been measured photometrically in dichloromethane. The resulting second-order rate constants correlate linearly with the electrophilicities E of the benzhydrylium ions which allowed us to use the correlation lg k = sN ( N + E) (eq 1) for determining the nucleophile-specific parameters N and sN of the diazo compounds. UV-vis spectroscopy was analogously employed to measure the rates of the 1,3-dipolar cycloadditions of these aryldiazomethanes with acceptor-substituted ethylenes of known electrophilicities E...
November 16, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/30443411/evaluation-of-intraductal-delivery-of-poly-ethylene-glycol-doxorubicin-conjugate-nanocarriers-for-the-treatment-of-ductal-carcinoma-in-situ-dcis-like-lesions-in-rats
#17
Zichao Gu, Firas Al-Zubaydi, Derek Adler, Shike Li, Steven Johnson, Puja Prasad, Jennifer Holloway, Zoltan Szekely, Susan Love, Dayuan Gao, Patrick J Sinko
Ductal carcinoma in situ is the most commonly diagnosed early stage breast cancer. The efficacy of intraductally delivered poly(ethylene glycol)-doxorubicin (PEG-DOX) nanocarriers, composed of one or more DOX conjugated to various PEG polymers, was investigated in an orthotopic ductal carcinoma in situ-like rat model. In vitro cytotoxicity was evaluated against 13762 Mat B III cells using MTT assay. The orthotopic model was developed by inoculating cancer cells into mammary ducts of female Fischer 344 retired breeder rats...
September 2018: Journal of Interdisciplinary Nanomedicine
https://www.readbyqxmd.com/read/30442442/a-comprehensive-study-on-the-main-physiological-and-biochemical-changes-occurring-during-growth-and-on-tree-ripening-of-two-apple-varieties-with-different-postharvest-behaviour
#18
Jordi Giné-Bordonaba, Gemma Echeverria, Elisabet Duaigües, Gloria Bobo, Christian Larrigaudière
Apple quality and the storage potential likely depend on a range of physiological and biochemical events occurring throughout fruit development and ripening. In this study, we investigated the major physiological (ethylene production and respiration) and biochemical changes (related to sugar and malic acid content as well as antioxidant metabolism) occurring during growth and on-tree ripening of two apple varieties ('Granny Smith' (GS) and 'Early Red One' (ERO)) with known differences in their postharvest behaviour, mainly firmness loss and susceptibility to superficial scald...
November 1, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/30417442/vitrification-of-equine-expanded-blastocysts-following-puncture-with-or-without-aspiration-of-the-blastocoele-fluid
#19
S Wilsher, F Rigali, G Couto, S Camargo, W R Allen
BACKGROUND: Historically, cryopreservation of equine embryos >300 μm gave poor pregnancy rates until researchers collapsed the blastocoele cavity and aspirated the blastocoele fluid prior to vitrification. OBJECTIVE: To determine if aspiration of the blastocoele fluid prior to vitrification is essential for post warming survival. STUDY DESIGN: In vivo experiments. METHODS: Fifty embryos were recovered on Day 7-8 and washed in holding medium (HM; M-199HEPES + 20% FBS + antibiotics)...
November 12, 2018: Equine Veterinary Journal
https://www.readbyqxmd.com/read/30415546/the-effect-of-formulation-and-processing-parameters-on-the-size-of-mpeg-b-p-hpma-bz-polymeric-micelles
#20
Mahsa Bagheri, Jaleesa Bresseleers, Aida Varela Moreira, Olivier Sandre, Silvie A Meeuwissen, Raymond M Schiffelers, Josbert M Metselaar, Cornelus F van Nostrum, Jan C M van Hest, Wim E Hennink
Micelles composed of block copolymers of poly(ethylene glycol)-b-poly(N-2-benzoyloxypropyl methacrylamide) (mPEG-b-p(HPMA-Bz)) have shown great promise as drug delivery carriers due to their excellent stability and high loading capacity. In the present study, parameters influencing micelle size were investigated to tailor sizes in the range of 25 to 100 nm. Micelles were prepared by a nanoprecipitation method and their size was modulated by the block copolymer properties such as molecular weight, their hydrophilic to hydrophobic ratio, homopolymer content, as well as formulation and processing parameters...
November 12, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
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