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https://www.readbyqxmd.com/read/29053867/molecular-cloning-and-functional-analysis-of-peafowl-pavo-cristatus-toll-like-receptor-7
#1
H Song, M Zhang, W Gao, L Wu, G Li
In order to clone the peafowl (Pavo cristatus) Toll-like receptor 7 (TLR7) gene and study its biological function, the peafowl TLR7 coding sequences (CDS) were amplified by PCR of cDNA from the whole spleen of peafowl. The full-length sequence of the peafowl TLR7 gene CDS is 3,141 bp and encodes a 1,046-amino acid protein with a classic TLR composition of 16 leucine-rich repeats (LRR). Insertions of amino acids were found at position 15 of LRR2, LRR5, LRR7, LRR9, LRR11, LRR12, LRR14, and LRR15; and position 10 of LRR11...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053676/laminotomy-for-lumbar-dorsal-root-ganglion-access-and-injection-in-swine
#2
Mark D Unger, Timothy P Maus, Ross C Puffer, Laura K Newman, Bradford L Currier, Andreas S Beutler
Dorsal root ganglia (DRG) are anatomically well defined structures that contain all primary sensory neurons below the head. This fact makes DRG attractive targets for injection of novel therapeutics aimed at treating chronic pain. In small animal models, laminectomy has been used to facilitate DRG injection because it involves surgical removal of the vertebral bone surrounding each DRG. We demonstrate a technique for intraganglionic injection of lumbar DRG in a large animal species, namely, swine. Laminotomy is performed to allow direct access to DRG using standard neurosurgical techniques, instruments, and materials...
October 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29052336/radiographic-and-clinical-outcomes-of-rooted-platform-switched-microthreaded-implants-with-a-sandblasted-large-grid-and-acid-etched-surface-a-5-year-prospective-study
#3
Dong Mei Mei, Baodong Zhao, Hao Xu, Yanhui Wang
BACKGROUND: There is no data available on the long-term outcomes of a rooted, platform-switched, microthreaded implant with a sandblasted, large-grid, and acid-etched surface. PURPOSE: This prospective, longitudinal study evaluated the clinical and radiographic outcomes of rooted, platform-switched, microthreaded and sandblasted, large-grid, and acid-etched (SLA) surface implants for 5 years. MATERIALS AND METHODS: Sixty implants were placed in sixty patients with partially edentulous maxillae or mandibles...
October 20, 2017: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/29049886/structure-activity-relationship-studies-of-pyrimido-5-4-b-indoles-as-selective-toll-like-receptor-4-ligands
#4
Michael Chan, Yuhei Kakitsubata, Tomoko Hayashi, Alast Ahmadi, Shiyin Yao, Nikunj M Shukla, Shin-Ya Oyama, Akihito Baba, Brandon Nguyen, Maripat Corr, Yasuo Suda, Dennis A Carson, Howard B Cottam, Masahiro Wakao
Previous high throughput screening studies led to the discovery of two novel, non-lipid-like chemotypes as Toll-like receptor 4 (TLR4) agonists. One of these chemotypes, the pyrimido[5,4-b]indoles, was explored for structure-activity relationship trends relative to production of TLR4 dependent cytokines/chemokines resulting in a semi-optimized lead (compound 1) that provided a starting point for further optimization studies. In this report, compounds belonging to three areas of structural modification were evaluated for biological activity using murine and human TLR4 reporter cells, primary murine bone marrow derived dendritic cells, and human peripheral blood mononuclear cells...
October 19, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29047405/cortisol-inhibits-mtor-signaling-in-avascular-necrosis-of-the-femoral-head
#5
Yun Liao, Rui Su, Ping Zhang, Bo Yuan, Ling Li
BACKGROUND: ANFH is a major health problem, to which long lasting and definitive treatments are lacking. The aim of this study is to study RNA alterations attributed to cortisol-induced ANFH. METHODS: Rat models were stratified into three groups: in vitro group (n = 20) for molecular biological assays, control group (n = 3), and ANFH group induced using lipopolysaccharide and dexamethasone (n = 3). Bone marrow-derived endothelial progenitor cells (BM-EPCs) were extracted from the rats...
October 18, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29047220/the-cytokines-and-microenvironment-of-fracture-haematoma-current-evidence
#6
REVIEW
Gavin Walters, Ippokratis Pountos, Peter V Giannoudis
BACKGROUND: Fracture haematoma formation is the first and foremost important stage of fracture healing. It orchestrates the inflammatory and cellular processes leading to the formation of callus and the restoration of the continuity of the bone. Evidence suggests that blocking this initial stage could lead to an impairment of the overall bone healing process. This review aims to analyse the existing evidence of molecular contributions to bone healing within fracture haematoma, and to determine the potential to modify the molecular response to fracture in the haematoma with the aim of improving union times...
October 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29046637/meclizine-prevents-ovariectomy-induced-bone-loss-and-inhibits-osteoclastogenesis-partially-by-upregulating-pxr
#7
Jiachao Guo, Weijin Li, Yingxing Wu, Xingzhi Jing, Junming Huang, Jiaming Zhang, Wei Xiang, Ranyue Ren, Zhengtao Lv, Jun Xiao, Fengjing Guo
Pregnane X receptor (PXR) which belongs to the nuclear hormone receptor superfamily plays vital roles in several biological functions, especially in the inflammatory procedure. Besides that, PXR is revealed by recent studies to have essential effects on bone tissue. As an agonist of PXR, meclizine is a piperazine-derived histamine H1 antagonist, and has been frequently used for prevention and treatment of vomiting and nausea. Because osteoclastogenesis is characterized by the activation of inflammation-related signaling pathways, we speculated that meclizine may affect formation and function of osteoclast...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29045624/long-term-cell-culture-isolated-from-lesions-of-mice-infected-with-leishmania-amazonensis-a-new-approach-to-study-mononuclear-phagocyte-subpopulations-during-the-infection
#8
Myriam Janeth Salazar Terreros, Luis Augusto Visani de Luna, Selma Giorgio
Leishmanioses are neglected diseases and the parasite Leishmania survives and proliferates within mononuclear phagocytes, particularly macrophages. In vitro studies of the immunology and cell biology of leishmaniosis are performed in murine peritoneum and bone marrow macrophages and immortalized cell lines despite the normal and injured tissue-specific heterogeneity of macrophages. In this work we established an ex vivo methodology to culture lesional cells from BALB/c mice infected with L. amazonensis. The cells were successfully isolated from footpad skin lesions and those exhibiting macrophage morphology were maintained in long-term culture (12 days), while the small number of lymphocytes, polymorphonuclear and unidentified cells died after one day of culture...
October 14, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/29044850/mesenchymal-stem-cells-therapy-a-promising-cell-based-therapy-for-treatment-of-myocardial-infraction
#9
REVIEW
Ayman El Sayed Shafei, Mahmoud Ahmed Ali, Hazem G Ghanem, Ahmed I Shehata, Ahmed A Abdelgawad, Hossam R Handal, Kareem A Talaat, Ahmed E Ashaal, Amal S El-Shal
AIM: For decades, mesenchymal stem (MSCs) cells had been used for cardiovascular diseases as regenerative therapy. This review is an attempt to summarize the types of MSCs involved in MI therapy, its possible mechanisms effects especially paracrine one in MI focusing on the studies (human and animal) conducted in the last 10 years. DISCUSSION: Recently, reports showed that MSC therapy could have infarct-limiting effects after myocardial infarction (MI) in both experimental and clinical trials...
October 16, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29044707/the-effect-of-vitamin-d-supplementation-on-bone-metabolic-markers-in-chronic-kidney-disease
#10
Ashok Kumar Yadav, Vivek Kumar, Vinod Kumar, Debasish Banerjee, Krishan Lal Gupta, Vivekanand Jha
Use of active forms of vitamin D is advocated in CKD patients for treatment of mineral bone disease because of the presumption that native forms of vitamin D would not undergo significant activation to calcitriol, the most active biological form of vitamin D. We present secondary analysis looking at bone turnover in subjects who completed the randomized, double blind, placebo controlled trial investigating the effect of cholecalciferol supplementation on vascular function in non-diabetic CKD stage G3-4 and vitamin D ≤20ng/ml [CTRI/2013/05/003648]...
October 17, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29044424/new-hope-for-symptom-management-during-natural-and-iatrogenic-menopause-transitions
#11
Teresa K Woodruff, Sundeep Khosla
Bone loss is associated with aging and is exacerbated by female transition to menopause. Estrogen has long been associated with bone loss, and replacement therapy is effective at restoring bone health. In the last 10 years, pituitary and gonadal peptide hormones have been implicated in bone biology, and here we update the story on FSH and its potential role as a direct regulator of bone and adipose. If translated to humans, new approaches to ameliorate age related bone demise could be developed. Moreover, young women with hormone responsive cancers who are unable to maintain bone may have new routes to avoid debilitating bone loss...
August 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29043108/mysterious-inhibitory-cell-regulator-investigated-and-found-likely-to-be-secretogranin-ii-related
#12
John E Hart, Iain J Clarke, Gail P Risbridger, Ben Ferneyhough, Mónica Vega-Hernández
In the context of a hunt for a postulated hormone that is tissue-mass inhibiting and reproductively associated, there is described probable relatedness to a granin protein. A 7-8 kDa polypeptide candidate (gels/MS) appeared in a bioassay-guided fractionation campaign involving sheep plasma. An N-terminal sequence of 14 amino acids was obtained for the polypeptide by Edman degradation. Bioinformatics and molecular biology failed to illuminate any ovine or non-ovine protein which might relate to this sequence...
2017: PeerJ
https://www.readbyqxmd.com/read/29042651/biochemistry-and-adaptive-colouration-of-an-exceptionally-preserved-juvenile-fossil-sea-turtle
#13
Johan Lindgren, Takeo Kuriyama, Henrik Madsen, Peter Sjövall, Wenxia Zheng, Per Uvdal, Anders Engdahl, Alison E Moyer, Johan A Gren, Naoki Kamezaki, Shintaro Ueno, Mary H Schweitzer
The holotype (MHM-K2) of the Eocene cheloniine Tasbacka danica is arguably one of the best preserved juvenile fossil sea turtles on record. Notwithstanding compactional flattening, the specimen is virtually intact, comprising a fully articulated skeleton exposed in dorsal view. MHM-K2 also preserves, with great fidelity, soft tissue traces visible as a sharply delineated carbon film around the bones and marginal scutes along the edge of the carapace. Here we show that the extraordinary preservation of the type of T...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29042277/mir-455-3p-activates-nrf2-are-signaling-via-hdac2-and-protects-osteoblasts-from-oxidative-stress
#14
Shijun Zhang, Wenliang Wu, Guangjun Jiao, Ci Li, Haichun Liu
BACKGROUND: The important role of miR-455-3p in the pathogenesis of bone metabolism associated diseases is gradually emerging. This study aims to ascertain the involvement of miR-455-3p and its underlying mechanisms in osteoporosis. METHODS: The osteoblast cell lines MC3T3-E1 was treated with ferric ammonium citrate (FAC) to mimic a pathological environment for osteoporosis. The cytotoxic effect of iron overload was assessed by proliferation, apoptosis and oxidative stress of osteoblasts using commercial kits...
October 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29042196/dcir3-and-dcir4-are-co-expressed-on-inflammatory-and-patrolling-monocytes
#15
Yu Hsu, Ryo Okada, Takashi Nishimura, Norihito Kawasaki, Kazuo Yamamoto, Naoki Matsumoto
Dendritic cell inhibitory receptor 3 (DCIR3) is a member of dendritic immuno-receptor family, of which protein expression has been unknown. We established a specific monoclonal antibody against mouse DCIR3 and investigated the expression of DCIR3 on immune cells of various immune organs. We found that DCIR3 was expressed on monocytes, but not on eosinophils and neutrophils. We also found the existence of a dichotomy in the levels of the expression of DCIR3 on monocytes in bone marrow, blood and spleen. Further investigation of the expression of several cell surface markers on DCIR3(High) cells and DCIR3(Low) cells revealed that DCIR3(High) cells were Ly-6C(-) CD43(High) CD11c(+) CD80(+) NK1...
October 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29040875/biomechanical-regulation-of-drug-sensitivity-in-an-engineered-model-of-human-tumor
#16
A Marturano-Kruik, A Villasante, K Yaeger, S R Ambati, A Chramiec, M T Raimondi, G Vunjak-Novakovic
Predictive testing of anticancer drugs remains a challenge. Bioengineered systems, designed to mimic key aspects of the human tumor microenvironment, are now improving our understanding of cancer biology and facilitating clinical translation. We show that mechanical signals have major effects on cancer drug sensitivity, using a bioengineered model of human bone sarcoma. Ewing sarcoma (ES) cells were studied within a three-dimensional (3D) matrix in a bioreactor providing mechanical loadings. Mimicking bone-like mechanical signals within the 3D model, we rescued the ERK1/2-RUNX2 signaling pathways leading to drug resistance...
October 10, 2017: Biomaterials
https://www.readbyqxmd.com/read/29039749/bionic-design-materials-and-performance-of-bone-tissue-scaffolds
#17
REVIEW
Tong Wu, Suihuai Yu, Dengkai Chen, Yanen Wang
Design, materials, and performance are important factors in the research of bone tissue scaffolds. This work briefly describes the bone scaffolds and their anatomic structure, as well as their biological and mechanical characteristics. Furthermore, we reviewed the characteristics of metal materials, inorganic materials, organic polymer materials, and composite materials. The importance of the bionic design in preoperative diagnosis models and customized bone scaffolds was also discussed, addressing both the bionic structure design (macro and micro structure) and the bionic performance design (mechanical performance and biological performance)...
October 17, 2017: Materials
https://www.readbyqxmd.com/read/29039747/development-of-useful-biomaterial-for-bone-tissue-engineering-by-incorporating-nano-copper-zinc-alloy-ncuzn-in-chitosan-gelatin-nano-hydroxyapatite-ch-g-nhap-scaffold
#18
Juan Carlos Forero, Eduardo Roa, Juan G Reyes, Cristian Acevedo, Nelson Osses
Ceramic and metallic nanoparticles can improve the mechanical and biological properties of polymeric scaffolds for bone tissue engineering (BTE). In this work, nanohydroxyapatite (nHAp) and nano-copper-zinc alloy (nCuZn) were added to a chitosan/gelatin (Ch/G) scaffold in order to investigate the effects on morphological, physical, and biocompatibility properties. Scaffolds were fabricated by a freeze-drying technique using different pre-freezing temperatures. Microstructure and morphology were studied by scanning electron microscopy (SEM), glass transition (Tg) was studied using differential scanning calorimetry (DSC), cell growth was estimated by MTT assay, and biocompatibility was examined in vitro and in vivo by histochemistry analyses...
October 17, 2017: Materials
https://www.readbyqxmd.com/read/29039539/adenoviral%C3%A2-bone-morphogenetic-protein%C3%A2-7-and-or-doxazosin-therapies-promote-the-reversion-of-fibrosis-cirrhosis-in-a-cirrhotic-hamster-model
#19
Daniel Cervantes-Garcia, Adriana Guadalupe Cuellar-Juarez, Gissela Borrego-Soto, Augusto Rojas-Martinez, Liseth Rubi Aldaba-Muruato, Eva Salinas, Javier Ventura-Juarez, Martin Humberto Muñoz-Ortega
Liver fibrosis occurs in the presence of continuous insults, including toxic or biological agents. Novel treatments must focus on ceasing the progression of cellular damage, promoting the regeneration of the parenchyma and inhibition of the fibrotic process. The present study analyzed the effect of bone morphogenetic protein (BMP)‑7 gene therapy with or without co‑treatment with doxazosin in a model of liver cirrhosis in hamsters. The serum alanine aminotransferase, aspartate aminotransferase and albumin levels were analyzed spectrophotometrically...
October 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29038849/malignant-bone-tumors-other-than-ewing-s-clinical-practice-guidelines-for-diagnosis-treatment-and-follow-up-by-spanish-group-for-research-on-sarcomas-geis
#20
Andrés Redondo, Silvia Bagué, Daniel Bernabeu, Eduardo Ortiz-Cruz, Claudia Valverde, Rosa Alvarez, Javier Martinez-Trufero, Jose A Lopez-Martin, Raquel Correa, Josefina Cruz, Antonio Lopez-Pousa, Aurelio Santos, Xavier García Del Muro, Javier Martin-Broto
Primary malignant bone tumors are uncommon and heterogeneous malignancies. This document is a guideline developed by the Spanish Group for Research on Sarcoma with the participation of different specialists involved in the diagnosis and treatment of bone sarcomas. The aim is to provide practical recommendations with the intention of helping in the clinical decision-making process. The diagnosis and treatment of bone tumors requires a multidisciplinary approach, involving as a minimum pathologists, radiologists, surgeons, and radiation and medical oncologists...
October 16, 2017: Cancer Chemotherapy and Pharmacology
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