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Protein status

Masafumi Otsuka, Tomohiko Kamasako, Toshihiro Uemura, Nobushige Takeshita, Tetsuo Shinozaki, Masayuki Kobayashi, Atsushi Komaru, Satoshi Fukasawa
OBJECTIVES: To elucidate the effects of the preoperative albumin : globulin ratio on the survival of patients with upper tract urothelial carcinoma after radical nephroureterectomy. METHODS: We retrospectively reviewed 124 consecutive patients with upper tract urothelial carcinoma who underwent radical nephroureterectomy at Chiba Cancer Center, Chiba, Japan between 2002 and 2015. The albumin : globulin ratio was defined: albumin / (total protein - albumin)...
August 13, 2018: International Journal of Urology: Official Journal of the Japanese Urological Association
Lei Zhang, Yufeng Wang, Liang Wang, Guozhi Yin, Weimin Li, Yao Xian, Wei Yang, Qingguang Liu
MicroRNAs (miRNAs) regulate a variety of development and physiologic processes, and play prominent roles in the initiation and progression of human cancers including hepatocellular carcinoma (HCC). MiR-23c is recently emerging as a cancer-associated miRNA, while its expression status and functional role in HCC are unrevealed yet. Here, we found that miR-23c underexpression was associated with the tumorigenesis of HCC based on TCGA data. qRT-PCR analysis revealed that miR-23c expression was reduced in HCC tissues and cell lines...
August 10, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Arumugam Rajavelu, Cristiana Lungu, Max Emperle, Michael Dukatz, Alexander Bröhm, Julian Broche, Ines Hanelt, Edris Parsa, Sarah Schiffers, Rahul Karnik, Alexander Meissner, Thomas Carell, Philipp Rathert, Renata Z Jurkowska, Albert Jeltsch
Despite their central importance in mammalian development, the mechanisms that regulate the DNA methylation machinery and thereby the generation of genomic methylation patterns are still poorly understood. Here, we identify the 5mC-binding protein MeCP2 as a direct and strong interactor of DNA methyltransferase 3 (DNMT3) proteins. We mapped the interaction interface to the transcriptional repression domain of MeCP2 and the ADD domain of DNMT3A and find that binding of MeCP2 strongly inhibits the activity of DNMT3A in vitro...
August 8, 2018: Nucleic Acids Research
Tiffany M N Otero, Cecilia Canales, D Dante Yeh, Ali Elsayes, Donna M Belcher, Sadeq A Quraishi
BACKGROUND: Hospital-acquired pressure injuries (HAPIs) typically develop following critical illness due to immobility and suboptimal perfusion. Vitamin D helps to maintain epithelial cell integrity, particularly at barrier sites such as skin. It is unclear whether vitamin D status is a modifiable risk factor for HAPIs in critically ill patients. Our goal was to investigate the relationship between admission 25-hydroxyvitamin D (25OHD) levels with the development of HAPIs in surgical intensive care unit (ICU) patients...
August 13, 2018: Nutrition in Clinical Practice
Julia B Greer, Phil Greer, Bimaljit S Sandhu, Samer Alkaade, C Mel Wilcox, Michelle A Anderson, Stuart Sherman, Timothy B Gardner, Michele D Lewis, Nalini M Guda, Thiruvengadam Muniraj, Darwin Conwell, Gregory A Cote, Christopher E Forsmark, Peter A Banks, Gong Tang, Kim Stello, Andres Gelrud, Randall E Brand, Adam Slivka, David C Whitcomb, Dhiraj Yadav
BACKGROUND: Chronic pancreatitis (CP) patients frequently experience malabsorption and maldigestion, leading to micronutrient and macronutrient deficiencies. Comorbid diabetes and lifestyle habits, such as alcohol consumption, may impact nutrition status. METHODS: We compared micronutrient antioxidant, bone metabolism, serum protein, and inflammatory marker levels in 301 CP patients and 266 controls with no known pancreatic disease. We analyzed serum prealbumin and retinol binding protein; vitamins A, D, E, and B12; osteocalcin; tumor necrosis factor-α; and C-reactive protein (CRP)...
August 13, 2018: Nutrition in Clinical Practice
Asako Goto, Mark Charman, Neale D Ridgway
Protein kinase D (PKD) controls secretion from the trans-Golgi network (TGN) by phosphorylating phosphatidylinositol 4-kinase IIIβ and proteins that bind and/or transfer phosphatidylinositol 4-phosphate (PTDINS-4P), such as oxysterol-binding protein (OSBP) and ceramide transfer protein. Here we investigated the consequences of PKD phosphorylation of OSBP at endoplasmic reticulum (ER)-Golgi membrane contact sites (MCS). Results with OSBP phospho-mutants revealed that PKD phosphorylation did not affect sterol and PTDINS-4P binding, activation of sphingomyelin (SM) synthesis at Golgi-ER MCS or other OSBP phospho-sites...
August 12, 2018: Traffic
Naoya Yamada, Takumi Katano, Yuta Hirata, Noriki Okada, Yukihiro Sanada, Yoshiyuki Ihara, Taizen Urahashi, Kentaro Ushijima, Tadayoshi Karasawa, Masafumi Takahashi, Koichi Mizuta
BACKGROUND: Serum Mac-2 Binding Protein Glycosylation isomer (M2BPGi) is a novel fibrosis marker for various chronic liver diseases. We investigated the ability of M2BPGi to predict liver fibrosis in liver transplant (LT) recipients. METHODS: A total of 116 liver biopsies (LB) were performed in 113 LT recipients. The serum level of M2BPGi was also measured on the same day. The median age at LT and LB was 1.1 and 11.8 years, respectively. Serum M2BPGi levels and liver fibrosis status using METAVIR fibrosis score were compared...
August 12, 2018: Journal of Gastroenterology and Hepatology
Tarek A Bismar, Samar Hegazy, Zhaoyong Feng, Darryl Yu, Bryan Donnelly, Nallasivam Palanisamy, Bruce J Trock
OBJECTIVES: To assess the prognostic value of ERG and PTEN protein expression as two of the most common genetic aberration in men with prostate cancer managed non-surgically by androgen deprivation therapy (ADT). MATERIALS AND METHODS: 463 tumor samples were assessed by double immunohistochemistry stains for ERG and PTEN and data correlated with clinical pathological features including, Gleason score, patients' outcome and ADT. RESULTS: ERG expression and PTEN protein loss were present in 28...
August 12, 2018: Journal of Cancer Research and Clinical Oncology
Richard J Edwards, Daniel Enosi Tuipulotu, Timothy G Amos, Denis O'Meally, Mark F Richardson, Tonia L Russell, Marcelo Vallinoto, Miguel Carneiro, Nuno Ferrand, Marc R Wilkins, Fernando Sequeira, Lee A Rollins, Edward C Holmes, Richard Shine, Peter A White
Background: The cane toad (Rhinella marina formerly Bufo marinus) is a species native to Central and South America that has spread across many regions of the globe. Cane toads are known for their rapid adaptation and deleterious impacts on native fauna in invaded regions. However, despite an iconic status, there are major gaps in our understanding of cane toad genetics. The availability of a genome would help to close these gaps and accelerate cane toad research. Findings: We report a draft genome assembly for R...
August 7, 2018: GigaScience
Xiliang Du, Taiyu Shen, Heyuan Wang, Xia Qin, Dongmei Xing, Qianqian Ye, Zhen Shi, Zhiyuan Fang, Yiwei Zhu, Yuchen Yang, Zhicheng Peng, Chenxu Zhao, Bin Lv, Xiaobing Li, Guowen Liu, Xinwei Li
The inevitable deficiency in nutrients and energy at the onset of lactation requires an optimal adaptation of the hepatic metabolism to overcome metabolic stress. Fatty liver is one of the main health disorders after parturition. Therefore, to investigate changes in hepatic lipid metabolic status and mitochondria in dairy cows with mild fatty liver, liver and blood samples were collected from healthy cows (n = 15) and cows with mild fatty liver (n = 15). To determine the effects of palmitic acids (PA), one of the major component of fatty acids, on lipid metabolism and mitochondria in vitro, calf hepatocytes were isolated from healthy calves and treated with various concentrations of PA (0, 50, 100, and 200 μM)...
August 9, 2018: Journal of Dairy Science
Masashi Ryo, Takafumi Yamashino, Hisanori Yamakawa, Yuichi Fujita, Setsuyuki Aoki
Two-component systems, versatile signaling mechanisms based on phosphate transfer between component proteins, must have played important roles in adaptation and diversification processes in land plant evolution. We previously demonstrated that two Per-Arnt-Sim (PAS)-histidine kinases, PHK1 and PHK2, repress gametophore formation in the moss Physcomitrella patens under aerobic conditions, and that, in eukaryotes, the presence of their homologs is restricted to early-diverging streptophyte linages. We assessed here whether or not PHKs play a role in oxygen signaling...
August 9, 2018: Biochemical and Biophysical Research Communications
Daisuke Tamura, Daichi Maeda, Sultan Ahmad Halimi, Masato Okimura, Yukitsugu Kudo-Asabe, Satoru Ito, Naoki Sato, Junji Shibahara, Hiroshi Nanjo, Yukihiro Terada, Akiteru Goto
AIMS: Uterine adenomatoid tumour (AT) is a benign proliferation of cells showing mesothelial differentiation within the myometrium that usually presents as a single nodule. Rare diffuse uterine ATs have been reported, often in patients undergoing immunosuppressive therapy. Herein, we aimed to elucidate the general association between the incidence of uterine AT and iatrogenic immunosuppression by cohort analysis. METHODS AND RESULTS: We analysed 611 consecutive hysterectomy specimens to determine the incidence of AT and its correlation with the immunosuppressive status...
August 11, 2018: Histopathology
Hellen Kuasne, Mateus C Barros-Filho, Fábio A Marchi, Sandra A Drigo, Cristovam Scapulatempo-Neto, Eliney F Faria, Silvia R Rogatto
Androgen receptor (AR) is a member of the steroid and nuclear family receptor that acts as transcription factor. AR signaling plays pivotal role in the development and progression of prostate cancer. However, the role of AR in penile cancer (PeCa) is poorly explored. Our previous molecular studies unveiled frequent AR mRNA loss in PeCa, which was further predicted as a major driver alteration in this neoplasm. Herein, we assessed the AR protein expression in 59 usual PeCa tissues and 42 surrounding normal tissues (SNT) by immunohistochemistry using a tissue microarray...
August 11, 2018: Virchows Archiv: An International Journal of Pathology
Yan Wang, Mengzhen Niu, Sha Yin, Fei Zhang, Ruizan Shi
BACKGROUND: To evaluate the protective effect of nebivolol against kidney damage and elucidate the underlying mechanism in a two-kidney, one-clip (2K1C) rat model. METHODS: 2K1C rats were obtained by clipping left renal artery of male Wistar rats and were considered hypertensive when systolic blood pressure (SBP) was ≥160 mmHg 4 weeks after surgery. The 2K1C hypertensive rats were divided into untreated, nebivolol (10 mg/kg, ig), and atenolol (80 mg/kg, ig) treatment groups...
April 13, 2018: Pharmacological Reports: PR
Giulia Boscutti, Eugenii A Rabiner, Christophe Plisson
The development of β+ emitter tracers labelled with carbon-11 or fluorine-18 having optimal characteristics of affinity and selectivity for the phosphodiesterase 10 A protein has received considerable attention, due to the major implication of this enzyme in neurological and psychiatric disorders, such as Hungtington's disease, Parkinson's disease and schizophrenia. The ability to quantify PDE10 A availability in the human brain in vivo will thus aid understanding its role in neurodegenerative and neuropsychiatric pathophysiology as well as providing a valuable tool for drug development...
August 9, 2018: Neuroscience Letters
Vidya Sagar Reddy, Brijesh Yadav, Chhote Lal Yadav, Mukul Anand, Dilip Kumar Swain, Devendra Kumar, Deepika Kritania, Arun Kumar Madan, Jitender Kumar, Sarvajeet Yadav
Cryopreservation results in substantial deterioration of heat shock protein 70 (HSP70) and ultra-structural changes in sperm organelles, resulting in a marked reduction in post-thaw semen quality. The present study was aimed to explicate the effect of sericin supplementation on expression profile of HSP70, redox status and post-thaw semen quality in Barbari goat. Five Barbari bucks were used to collect thirty semen ejaculates by using artificial vagina and each ejaculate was divided into three aliquots to which sericin was supplemented at 0% (Control), 0...
August 9, 2018: Cryobiology
Chao Li, Qian Ni, Yifang Pei, Yi Ren, Yufei Feng
BACKGROUND & AIMS: We performed a meta-analysis of data from recent studies to evaluate the safety and efficacy of parenteral nutrition (PN) with structured triglyceride (STG) lipid emulsions compared to medium-chain triglyceride (MCT)/long-chain triglyceride (LCT) lipid emulsions in Chinese patients. METHODS: PubMed, Embase, Cochrane Library, China National Knowledge Internet, Wanfang, and VIP were searched for randomized controlled trials comparing STGs with MCTs/LCTs published in English or Chinese between January 1987 and October 2017...
July 25, 2018: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
Matt Lechner, Ankur R Chakravarthy, Vonn Walter, Liam Masterson, Andrew Feber, Amrita Jay, Paul M Weinberger, Richard A McIndoe, Cillian T Forde, Kerry Chester, Nicholas Kalavrezos, Paul O'Flynn, Martin Forster, Terry M Jones, Francis M Vaz, D Neil Hayes, Tim R Fenton
OBJECTIVES: p16INK4A (p16) is the most widely used clinical biomarker for Human Papillomavirus (HPV) in head and neck squamous cell cancer (HNSCC). HPV is a favourable prognostic marker in HNSCC and is used for patient stratification. While p16 is a relatively accurate marker for HPV within the oropharynx, recent reports suggest it may be unsuitable for use in other HNSCC subsites, where a smaller proportion of tumors are HPV-driven. MATERIALS AND METHODS: We integrated reverse phase protein array (RPPA) data for p16 with HPV status based on detection of viral transcripts by RNA-seq in a set of 210 HNSCCs profiled by The Cancer Genome Atlas project...
August 2018: Oral Oncology
Md Sakhawat Hossain, Shunsuke Koshio, Manabu Ishikawa, Saichiro Yokoyama, Nadia Mahjabin Sony, Serge Dossou, Weilong Wang
Both inosine (INO) and vitamin C (l-ascorbic acid, AsA) play important roles in growth performance, feed utilization and health status of fish. Therefore, a 56 days feeding trial was conducted to determine the interactive effects of dietary INO and AsA on growth performance, oxidative status, innate and adaptive immune responses of red sea bream. Fish growth performance and fed utilization parameters were significantly affected by dietary INO supplementation but not by AsA. Fish fed diets with INO at 4 g kg-1 diet in combination of high and low levels of AsA (3...
August 9, 2018: Fish & Shellfish Immunology
Saeed Khoshnood, Mohsen Heidary, Mehri Haeili, Michel Drancourt, Davood Darban-Sarokhalil, Mohammad Javad Nasiri, Vahid Lohrasbi
Tuberculosis (TB) is now among the top ten causes of mortality worldwide being resulted in 1.7 million deaths in 2016. The Bacille Calmette-Guerin (BCG) is the only available TB vaccine which fails to provide consistent protection against pulmonary TB in adults and adolescents despite being efficacious at protecting infants and young children from the most severe, often deadly forms of TB disease. To achieve the goal of global TB elimination by 2050 we will need new interventions including more improved vaccines that are effective in adult individuals who have not been infected with Mycobacterium tuberculosis as well as latently infected or immunocompromised subjects...
August 8, 2018: International Journal of Biological Macromolecules
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