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Methylation promoter

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https://www.readbyqxmd.com/read/30322210/camp-protein-kinase-a-signaling-inhibits-dlx5-expression-via-activation-of-creb-and-subsequent-c-ebp%C3%AE-induction-in-3t3-l1-preadipocytes
#1
Hye-Lim Lee, Abdul S Qadir, Hyun-Jung Park, Eunkyung Chung, Yun-Sil Lee, Kyung Mi Woo, Hyun-Mo Ryoo, Hyun Jeong Kim, Jeong-Hwa Baek
Distal-less homeobox 5 (Dlx5) is a negative regulator of adipogenesis. Dlx5 expression is decreased by adipogenic stimuli, but the mechanisms of Dlx5 downregulation by adipogenic stimuli have not yet been determined. Here, we tested the impact of cAMP/PKA (protein kinase A) signaling induced by 3-isobutyl-1 methyl xanthine (IBMX), forskolin, and 8-CPT-cAMP on the expression of Dlx5 in 3T3-L1 preadipocytes. Significant downregulation of Dlx5 mRNA expression and protein production levels were observed via cAMP/PKA-dependent signaling...
October 14, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30320910/interactome-analysis-of-the-human-cap-specific-mrna-nucleoside-2-o-methyltransferase-1-hmtr1-protein
#2
Fernando Moreira Simabuco, Isadora Carolina Betim Pavan, Nathalie Fortes Pestana, Paulo Costa Carvalho, Fernanda Luisa Basei, Daniela Campos Granato, Adriana Franco Paes Leme, Nilson Ivo Tonin Zanchin
In a previous study, we have shown that the gene promoter of a protein termed KIAA0082 is regulated by interferon and that this protein interacts with the RNA polymerase II. It has been subsequently shown that KIAA0082 is the human cap-specific messenger RNA (mRNA) (nucleoside-2'-O-)-methyltransferase 1 (hMTr1), which catalyzes methylation of the 2'-O -ribose of the first nucleotide of capped mRNAs. Pre-mRNAs are cotranscriptionally processed, requiring coordinate interactions or dissociations of hundreds of proteins...
October 15, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/30320343/phosphorylation-of-nucleolin-is-indispensable-to-its-involvement-in-the-proliferation-and-migration-of-non-small-cell-lung-cancer-cells
#3
Feifei Huang, Yanyang Wu, Hong Tan, Tianyao Guo, Ke Zhang, Daiqiang Li, Zhongyi Tong
Non-small cell lung cancer (NSCLC) is one of the mostly deadly malignancies in the world. Nucleolin is a multifunctional protein that mainly regulates ribosome biogenesis but also has other functions including modulating the transcription of mRNAs and repressing RNA polymerase II. Nucleolin is overexpressed in various cancer cells, including NSCLC cells. It can confer resistance to apoptosis and promote cell migration and blood vessel formation by directly taking part in various tumor signal transduction pathways...
October 12, 2018: Oncology Reports
https://www.readbyqxmd.com/read/30320192/anti-endothelial-cell-antibody-rich-sera-from-rheumatic-heart-disease-patients-induces-proinflammatory-phenotype-and-methylation-alteration-in-endothelial-cells
#4
Mukul Rastogi, Subendu Sarkar, Ankita Makol, Rana Sandip Singh, Uma Nahar Saikia, Dibyajyoti Banerjee, Seema Chopra, Anuradha Chakraborti
Rheumatic heart disease (RHD) is a major cause of cardiovascular morbidity and mortality in developing nations like India. RHD commonly affects the mitral valve which is lined by a single layer of endothelial cells (ECs). The role of ECs in mitral valve damage during RHD is not well elucidated. In here, anti-endothelial cell antibody from RHD patients has been used to stimulate the ECs (HUVECs and HMVECs). ECs proinflammatory phenotype with increased expression of TNFα, IL-6, IL-8, IFNγ, IL-1β, ICAM1, VCAM1, E-selectin, laminin B, and vimentin was documented in both ECs...
September 2018: Genes & Diseases
https://www.readbyqxmd.com/read/30318121/mir-93-5p-suppresses-cellular-senescence-by-directly-targeting-bcl-w-and-p21
#5
Jae Yeon Choi, Hyun Jin Shin, In Hwa Bae
Cellular senescence, a distinctive type of irreversible growth arrest, develops in response to various stimuli. Bcl-w, an oncogene and member of the Bcl-2 family, has been reported to promote tumorigenicity in various cancer cells. Here, we sought to explore the potential role of Bcl-w in premature senescence, which has received relatively little research attention. Our findings demonstrate that Bcl-w enhances the activity of senescence-associated β-galactosidase (SA-β-gal) and promotes histone H3 tri-methylation at lysine 9 (H3K9me3) and expressions of p53, Notch2, p21, and p16-hallmarks of the senescent phenotype-in human U251 glioblastoma and H460 lung carcinoma cells...
October 11, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/30317626/hypermethylation-of-itgbl1-is-associated-with-poor-prognosis-in-acute-myeloid-leukemia
#6
Xin-Yue Lian, Ji-Chun Ma, Jing-Dong Zhou, Ting-Juan Zhang, De-Hong Wu, Zhao-Qun Deng, Zhi-Hui Zhang, Xi-Xi Li, Pin-Fang He, Yang Yan, Jiang Lin, Jun Qian
The current study was aimed to investigate integrin beta-like 1 (ITGBL1) methylation pattern and its clinical relevance in patients with acute myeloid leukemia (AML). Real-time methylation-specific polymerase chain reaction (PCR; RQ-MSP) and bisulfite sequencing PCR (BSP) were performed to detect the methylation of ITGBL1 promoter. Real-time quantitative PCR (RT-qPCR) was performed to analyze ITGBL1 transcript level. The results showed that ITGBL1 methylation level in 131 patients with AML was significantly higher than 29 controls (p < 0...
October 14, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30317605/lin28a-activates-the-transcription-of-nanog-in-dairy-goat-male-germline-stem-cells
#7
REVIEW
Fanglin Ma, Xiaomin Du, Yudong Wei, Zhe Zhou, Daguia Zambe John Clotaire, Na Li, Sha Peng, Guangpeng Li, Jinlian Hua
LIN28A serves as a crucial marker of dairy goat male germline stem cells (GmGSCs). In our previous study, we demonstrated that LIN28A promotes proliferation, self-renewal, and maintains the stemness of GmGSCs. Here, we found that LIN28A could activate the transcription of NANOG in a let-7g independent manner. We cloned the 5' upstream of two NANOG genes which were located on chromosome 15 ( NANOG-ch15) and chromosome 5 ( NANOG-ch5), respectively, and then examined their promoter activities and promoter methylation levels...
October 14, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30317601/exploring-and-comparing-of-the-gene-expression-and-methylation-differences-between-lung-adenocarcinoma-and-squamous-cell-carcinoma
#8
Yang Yang, Meng Wang, Bao Liu
Lung cancer is one of the most frequently diagnosed malignant tumors and the main reason for cancer-related death around the world, whereas nonsmall cell lung cancer that consists two subtypes: lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) is responsible for an estimated 85% of all lung cancers. The current study aimed to explore gene expression and methylation differences between LUAD and LUSC. EdgeR was used to identify differentially regulated genes between normal and cancer in the LUAD and LUSC extracted from The Cancer Genome Atlas (TCGA), respectively, whereas SAM was used to find genes with differential methylation between normal and cancer in the LUAD and LUSC, respectively...
October 14, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30316843/2-6-dimethoxy-1-4-benzoquinone-isolation-and-identification-of-anti-carcinogenic-anti-mutagenic-and-anti-inflammatory-component-from-the-juice-of-vitis-coignetiae
#9
Tomonori Kamiya, Yusuke Tanimoto, Nana Fujii, Tomoe Negishi, Toshinori Suzuki, Tsutomu Hatano, Sakae Arimoto-Kobayashi
Previously we demonstrated the anti-tumorigenic, anti-mutagenic and anti-inflammatory effects of the juice of Vitis coignetiae (yamabudo), and identified caftaric acid as an anti-mutagenic component from the juice. In the present study, we investigated the isolation of anti-inflammatory components in yamabudo juice supposing that the anti-inflammatory components in yamabudo are also responsible for the anti-tumorigenic activity. The suppressing effect on nitric oxide production in mouse leukemic monocyte with LPS was used as a separation marker...
October 11, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/30315899/identification-of-dna-methylation-regulated-novel-host-genes-relevant-to-inhibition-of-virus-replication-in-porcine-pk15-cell-using-double-stranded-rna-mimics-and-dna-methyltransferase-inhibitor
#10
Xiaoshuo Wang, Hong Ao, Minyan Song, Lijing Bai, Weiyong He, Chuduan Wang, Ying Yu
During RNA viruses's replication, double-stranded RNA (dsRNA) is normally produced and induce host innate immune response. Most of gene activation due cytokine mediated but which are due to methylation mediated is still unknown. In the study, DNA methylome was integrated with our previous transcriptome data to investigate the differentially methylated regions and genes using MeDIP-chip technology. We found that the transcriptional expressions of 15, 37 and 18 genes were negatively related with their promoter DNA methylation levels in the cells treated by PolyI:C, Aza-CdR, as well as PolyI:C plus Aza-CdR, respectively, compared with the untreated cells...
October 10, 2018: Genomics
https://www.readbyqxmd.com/read/30315898/dynamic-alterations-in-methylation-of-global-dna-and-growth-related-genes-in-large-yellow-croaker-larimichthys-crocea-in-response-to-starvation-stress
#11
Yu Zhang, Weiliang Shen, Mingyue Cao, Juan Li, Baoxiao Zheng, Zhengjia Lou, Baoying Qian, Liangyi Xue
DNA methylation is susceptible to various environmental factors such as salinity, temperature and nutritional conditions, and can affect gene function, organ metabolism, body growth and development. In order to explore the effect of starvation on growth-related genes in large yellow croaker (Larimichthys crocea), we studied methylation of the global DNA and growth-related genes (MSTN1,MSTN2,IGF1,IGF2) and the corresponding mRNA expressions, using ELISA-based technique, bisulfite sequencing PCR (BSP) technique and quantitative Real-time PCR (qRT-PCR) respectively...
October 10, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/30315258/atrx-daxx-or-men1-mutant-pancreatic-neuroendocrine-tumors-are-a-distinct-alpha-cell-signature-subgroup
#12
Chang S Chan, Saurabh V Laddha, Peter W Lewis, Matthew S Koletsky, Kenneth Robzyk, Edaise Da Silva, Paula J Torres, Brian R Untch, Janet Li, Promita Bose, Timothy A Chan, David S Klimstra, C David Allis, Laura H Tang
The commonly mutated genes in pancreatic neuroendocrine tumors (PanNETs) are ATRX, DAXX, and MEN1. We genotyped 64 PanNETs and found 58% carry ATRX, DAXX, and MEN1 mutations (A-D-M mutant PanNETs) and this correlates with a worse clinical outcome than tumors carrying the wild-type alleles of all three genes (A-D-M WT PanNETs). We performed RNA sequencing and DNA-methylation analysis to reveal two distinct subgroups with one consisting entirely of A-D-M mutant PanNETs. Two genes differentiating A-D-M mutant from A-D-M WT PanNETs were high ARX and low PDX1 gene expression with PDX1 promoter hyper-methylation in the A-D-M mutant PanNETs...
October 12, 2018: Nature Communications
https://www.readbyqxmd.com/read/30315244/cirbp-is-a-novel-oncogene-in-human-bladder-cancer-inducing-expression-of-hif-1%C3%AE
#13
Mengxin Lu, Qiangqiang Ge, Gang Wang, Yongwen Luo, Xiaolong Wang, Wei Jiang, Xuefeng Liu, Chin-Lee Wu, Yu Xiao, Xinghuan Wang
Cold-inducible RNA binding protein (CIRBP) has been reported to be associated with distinct tumorigenesis. In this study, we investigated the role of CIRBP in human bladder cancer (BCa), indicating that CIRBP is overexpressed in BCa tissues and cell lines to promote proliferation and migration. Moreover, CIRBP could induce expression of HIF-1α via binding to the 3'-UTR of its mRNA to increase the mRNA stability in BCa cells. Furthermore, we demonstrated that PTGIS is a HIF-1α targeted gene, a major regulator in hypoxic cancer progression by activating transcription of various oncogenes...
October 12, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/30311169/epigenetic-status-of-cdo1-gene-may-reflect-chemosensitivity-in-colon-cancer-with-postoperative-adjuvant-chemotherapy
#14
Keigo Yokoi, Hiroki Harada, Kazuko Yokota, Satoru Ishii, Toshimichi Tanaka, Nobuyuki Nishizawa, Masashi Shimazu, Ken Kojo, Hirohisa Miura, Takahiro Yamanashi, Takeo Sato, Takatoshi Nakamura, Masahiko Watanabe, Keishi Yamashita
BACKGROUND: Cysteine dioxygenase type 1 (CDO1) acts as a tumor suppressor gene, and its expression is regulated by promoter DNA methylation in human cancer. The metabolic product mediated by CDO1 enzyme increases mitochondrial membrane potential (MMP), putatively representing chemoresistance. The aim of this study is to investigate the functional relevance of CDO1 gene in colon cancer with chemotherapy. PATIENTS AND METHODS: We investigated 170 stage III colon cancer patients for CDO1 methylation by using quantitative methylation-specific polymerase chain reaction (PCR)...
October 11, 2018: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/30310577/br%C3%A3-nsted-lewis-acid-sites-synergistically-promote-the-initial-c-c-bond-formation-in-the-mto-reaction
#15
Yueying Chu, Xianfeng Yi, Chengbin Li, Xianyong Sun, Anmin Zheng
The methanol-to-olefin (MTO) reaction is an active field of research due to conflicting mechanistic proposals for the initial carbon-carbon (C-C) bond formation. Herein, a new methane-formaldehyde pathway, a Lewis acid site combined with a Brønsted acid site in zeolite catalysts can readily activate dimethyl ether (DME) to form ethene, is identified theoretically. The mechanism involves a hydride transfer from Al-OCH3 on the Lewis acid site to the methyl group of the protonated methanol molecule on the adjacent Brønsted acid site leading to synchronous formation of methane and Al-COH2 + (which can be considered as formaldehyde (HCHO) adsorbed on the Al3+ Lewis acid sites)...
August 21, 2018: Chemical Science
https://www.readbyqxmd.com/read/30310175/bcat1-and-mir-2504-novel-methylome-signature-distinguishes-spindle-desmoplastic-melanoma-from-superficial-malignant-peripheral-nerve-sheath-tumor
#16
George Jour, Varshini Vasudevaraja, Victor G Prieto, Matija Snuderl, Carlos A Torres-Cabala, Rami Al-Rohil, Erik P Sulman, Leomar Y Ballester, Phyu P Aung
Superficial/cutaneous malignant peripheral nerve sheath tumor is a rare soft tissue neoplasm that shares morphological, immunohistochemical, and molecular features with spindle/desmoplastic melanoma. We aimed to identify a methylome signature to distinguish these two entities. We analyzed 15 cases of spindle/desmoplastic melanoma and 15 cases of cutaneous malignant peripheral nerve sheath tumor in 23 men and 7 women. DNA from formalin-fixed, paraffin-embedded tissues was extracted and processed using the Illumina Infinium Methylation EPIC array interrogating 866,562 CpG sites...
October 11, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/30309962/discovery-of-a-small-molecule-inhibitor-of-specific-serine-residue-bad-phosphorylation
#17
Vijay Pandey, Baocheng Wang, Chakrabhavi Dhananjaya Mohan, Ainiah Rushdiana Raquib, Shobith Rangappa, Venkatachalaiah Srinivasa, Julian E Fuchs, Kesturu S Girish, Tao Zhu, Andreas Bender, Lan Ma, Zhinan Yin, Basappa, Kanchugarakoppal S Rangappa, Peter E Lobie
Human BCL-2-associated death promoter (hBAD) is an apoptosis-regulatory protein mediating survival signals to carcinoma cells upon phosphorylation of Ser99, among other residues. Herein, we screened multiple small-molecule databases queried in a Laplacian-modified naive Bayesian-based cheminformatics platform and identified a Petasis reaction product as a site-specific inhibitor for hBAD phosphorylation. Based on apoptotic efficacy against mammary carcinoma cells, N -cyclopentyl-3-((4-(2,3-dichlorophenyl) piperazin-1-yl) (2-hydroxyphenyl) methyl) benzamide (NPB) was identified as a potential lead compound...
October 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/30309499/anti-aging-protective-effect-of-l-carnitine-as-clinical-agent-in-regenerative-medicine-through-increasing-telomerase-activity-and-change-in-the-htert-promoter-cpg-island-methylation-status-of-adipose-tissue-derived-mesenchymal-stem-cells
#18
Raheleh Farahzadi, Ezzatollah Fathi, Seyed Alireza Mesbah-Namin, Nosratollah Zarghami
The identification of factors that reduce the senescent tendency of the mesenchymal stem cells (MSCs) upon expansion has great potential for cellular therapies in regenerative medicine. Previous studies have shown the aging protective effect of L-carnitine (LC). On the other hand, reduction in proliferation potential and age-dependent decline in number and functions of MSCs were accompanied by telomere shortening, reduction in telomerase activity and epigenetic changes. The aim of this study was to evaluate the effects of LC on aging of MSCs through telomerase activity assessment and the investigation of methylation status of the hTERT gene promoter...
October 2018: Tissue & Cell
https://www.readbyqxmd.com/read/30309230/aberrant-promoter-hypomethylation-of-sortilin-1-a-moyamoya-disease-biomarker
#19
Hye Youn Sung, Ji Yeoun Lee, Ae Kyung Park, Youn Joo Moon, Inho Jo, Eun-Mi Park, Kyu-Chang Wang, Ji Hoon Phi, Jung-Hyuck Ahn, Seung-Ki Kim
BACKGROUND AND PURPOSE: The pathogenesis of moyamoya disease (MMD) remains poorly understood, and no reliable molecular biomarkers for MMD have been identified to date. The present study aimed to identify epigenetic biomarkers for use in the diagnosis of MMD. METHODS: We performed integrated analyses of gene expression profiles and DNA methylation profiles in endothelial colony forming cells (ECFCs) from three patients with MMD and two healthy individuals. Candidate gene mRNA expression and DNA methylation status were further validated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and pyrosequencing analysis of an expanded ECFC sample set from nine patients with MMD and ten controls...
September 2018: Journal of Stroke
https://www.readbyqxmd.com/read/30308939/hypermethylation-of-ccnd2-in-lung-and-breast-cancer-is-a-potential-biomarker-and-drug-target
#20
Chin-Sheng Hung, Sheng-Chao Wang, Yi-Ting Yen, Tzong-Huei Lee, Wu-Che Wen, Ruo-Kai Lin
Lung and breast cancer are the leading causes of mortality in women worldwide. The discovery of molecular alterations that underlie these two cancers and corresponding drugs has contributed to precision medicine. We found that CCND2 is a common target in lung and breast cancer. Hypermethylation of the CCND2 gene was reported previously; however, no comprehensive study has investigated the clinical significance of CCND2 alterations and its applications and drug discovery. Genome-wide methylation and quantitative methylation-specific real-time polymerase chain reaction (PCR) showed CCND2 promoter hypermethylation in Taiwanese breast cancer patients...
October 10, 2018: International Journal of Molecular Sciences
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