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https://www.readbyqxmd.com/read/30019367/gc-ms-aroma-characterization-of-vegetable-matrices-focus-on-3-alkyl-2-methoxypyrazines
#1
D Mutarutwa, L Navarini, V Lonzarich, D Compagnone, P Pittia
3-alkyl-2-methoxypyrazines (MPs) are a very important class of volatile organic compounds (VOCs), intensively characterizing the aroma of several food products including fruits and vegetables. Due to the very low orthonasal sensory threshold, low amounts of MPs may act as key positive or negative aroma compounds. The analysis of these volatiles in foods are, then, remarkably important and it may be very challenging as confirmed by the scarce literature focused on MPs, particularly with respect to quantitative data...
July 17, 2018: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/29896953/chemical-and-sensory-evaluation-of-magnetic-polymers-as-a-remedial-treatment-for-elevated-concentrations-of-3-isobutyl-2-methoxypyrazine-in-cabernet-sauvignon-grape-must-and-wine
#2
Chen Liang, Renata Ristic, Vladimir Jiranek, David W Jeffery
3-Isobutyl-2-methoxypyrazine (IBMP) is a potent odorant present in grapes and wines that is reminiscent of green capsicum. Suprathreshold concentrations can lead to obvious vegetative characters and suppress desirable fruity aroma nuances in wines, but options to manage IBMP concentrations are limited. This work investigated pre- and postfermentation addition of a putative imprinted magnetic polymer (PIMP) as a remedial treatment for elevated concentrations of IBMP in Cabernet Sauvignon grape must in comparison to nonimprinted magnetic polymer (NIMP) and to a commercially available polylactic acid (PLA) based film added postfermentation...
July 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29748522/preparation-of-magnetic-polymers-for-the-elimination-of-3-isobutyl-2-methoxypyrazine-from-wine
#3
Chen Liang, David W Jeffery, Dennis K Taylor
3-Isobutyl-2-methoxypyrazine (IBMP), the most prevalent grape-derived methoxypyrazine, can contribute green bell pepper, vegetative and herbaceous aromas and flavours to wines. At elevated concentrations, this potent odorant may mask desirable fruity and floral aromas in wine and may be considered as a fault. A new remediation method for wines with elevated IBMP levels has been trialled using magnetic polymers, prepared in the same way as ordinary polymers but with the incorporation of iron oxide nanoparticles as magnetic substrates...
May 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29597302/effects-of-basal-defoliation-on-wine-aromas-a-meta-analysis
#4
REVIEW
Yu Wang, Lei He, Qiuhong Pan, Changqing Duan, Jun Wang
Basal defoliation, as one of the most common viticulture management practices to modify fruit zone microclimates, has been widely applied aiming at improving wine quality. Wine aroma contributes greatly to wine quality, yet the effects of basal defoliation on wine aromas show discrepancies according to previous studies. This study is a meta-analysis performed to dissect the factors related to the influence of basal defoliation on volatile compounds in wine. Timing of basal defoliation plays an important role in the concentration of varietal aromas in wine...
March 28, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29479808/2-alkyl-3-methoxypyrazines-are-potent-attractants-of-florivorous-scarabs-melolonthidae-cyclocephalini-associated-with-economically-exploitable-neotropical-palms-arecaceae
#5
Artur Campos Dália Maia, Geanne Karla Novais Santos, Eduardo Gomes Gonçalves, Daniela Maria do Amaral Ferraz Navarro, Luis Alberto Nuñez-Avellaneda
BACKGROUND: 2-Alkyl-3-methoxypyrazines (MPs) are naturally occurring aromatic compounds involved in insect chemical communication as both pheromones and allelochemicals. Although rarely characterized in floral scents, they have been identified as major constituents in headspace samples from palm inflorescences and evidence pointed towards their function as attractive cues for scent-oriented pollinators, as well as florivores. In this study, we investigated the occurrence of MPs in economically exploitable palms belonging to Acrocomia and Attalea through headspace floral scent analysis and assessed their role in the selective attraction of flower-feeding cyclocephaline scarabs (Melolonthidae, Cyclocephalini) in field bioassays conducted in Brazil and Colombia...
February 25, 2018: Pest Management Science
https://www.readbyqxmd.com/read/29414884/degradation-investigation-of-selected-taste-and-odor-compounds-by-a-uv-chlorine-advanced-oxidation-process
#6
Jingyun Fang, Jiajian Liu, Chii Shang, Chihhao Fan
Taste- and odor-causing (T&O) compounds are a major concern in drinking water treatment plants due to their negative impacts on the safety and palatability of water supply. This study explored the degradation kinetics and radical chemistry of four often-detected T&O compounds, geosmin (GSM), 2-methylisoborneol (MIB), benzothiazole (BT), and 2-isobutyl-3-methoxypyrazine (IBMP), in the ultraviolet/chlorine (UV/chlorine) advanced oxidation process. All experiments were carried out in a 700 mL photoreactor and the process effectively degraded the investigated T&O compounds in a slightly acidic environment...
February 7, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29274583/a-stability-study-of-expired-ampoules-manufactured-more-than-40-years-ago
#7
Markus Zilker, Fritz Sörgel, Ulrike Holzgrabe
Pharmaceutical manufacturers have to study the stability of drug products before marketing according to ICH guideline Q1A(R2); data of those investigations aim to set expiry dates. The expiry date on the container of a remedy assures the physician and the patient a stability of the drug in its formulation i.e. within a specification of 95-105%. Only few studies show that shelf-lives of pharmaceutical products are often longer than expiration dates. The objective of the study presented here was determining the content of nine expired ampoules manufactured in the last century and identifying the impurity profile by means of HPLC-UV and HPLC-MS, respectively...
February 20, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28724556/performance-assessment-of-a-trypanosoma-cruzi-chimeric-antigen-in-multiplex-liquid-microarray-assays
#8
Fred Luciano Neves Santos, Paola Alejandra Fiorani Celedon, Nilson Ivo Tonin Zanchin, Amanda Leitolis, Sandra Crestani, Leonardo Foti, Wayner Vieira de Souza, Yara de Miranda Gomes, Marco Aurélio Krieger
Diagnosing chronic Chagas disease (CD) requires antibody-antigen detection methods, which are traditionally based on enzymatic assay techniques whose performance depend on the type and quality of antigen used. Previously, 4 recombinant chimeric proteins from the Instituto de Biologia Molecular do Paraná (IBMP-8.1 to 8.4) comprising immuno-dominant regions of diverse Trypanosoma cruzi antigens showed excellent diagnostic performance in enzyme-linked immunosorbent assays. Considering that next-generation platforms offer improved CD diagnostic accuracy with different T...
October 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28273127/accuracy-of-chimeric-proteins-in-the-serological-diagnosis-of-chronic-chagas-disease-a-phase-ii-study
#9
COMPARATIVE STUDY
Fred Luciano Neves Santos, Paola Alejandra Fiorani Celedon, Nilson Ivo Tonin Zanchin, Wayner Vieira de Souza, Edimilson Domingos da Silva, Leonardo Foti, Marco Aurélio Krieger, Yara de Miranda Gomes
BACKGROUND: The performance of current serologic tests for diagnosing chronic Chagas disease (CD) is highly variable. The search for new diagnostic markers has been a constant challenge for improving accuracy and reducing the number of inconclusive results. METHODOLOGY/PRINCIPAL FINDINGS: Here, four chimeric proteins (IBMP-8.1 to -8.4) comprising immunodominant regions of different Trypanosoma cruzi antigens were tested by enzyme-linked immunosorbent assay. The proteins were used to detect specific anti-T...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28060498/1-8-cineole-in-french-red-wines-evidence-for-a-contribution-related-to-its-various-origins
#10
Xavier Poitou, Cécile Thibon, Philippe Darriet
The aromatic descriptor "green", reflecting grape unripeness in French red wines, is frequently associated with the levels of 3-alkyl-2-methoxypyrazines, particularly 3-isobutyl-2-methoxypyrazine (IBMP), which has bell pepper nuances. Nevertheless, not all green aromatic expressions in red wines correlate with 3-alkyl-2-methoxypyrazine concentrations. This study considered sensory and chemical approaches using Cabernet Sauvignon wines obtained from grapes harvested at one-month intervals during the 2014 and 2015 vintages to investigate other volatile odoriferous compounds...
January 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27844316/occurrence-and-distribution-of-taste-and-odor-compounds-in-subtropical-water-supply-reservoirs-and-their-fates-in-water-treatment-plants
#11
Xiuzhi Bai, Ting Zhang, Chaoyi Wang, Dongliang Zong, Haipu Li, Zhaoguang Yang
Taste and odor (T&O) problems in surface water supplies attract growing environmental and ecological concerns. In this study, 10 T&O compounds, 2-methylisoborneol (2-MIB), geosmin, β-ionone, 2-isopropyl-3-methoxypyrazine (IPMP), 2-isobutyl-3-methoxypyrazine (IBMP), 2,4,6-trichloroanisole (2,4,6-TCA), 2,3,6-trichloroanisole (2,3,6-TCA), 2,3,4-trichloroanisole (2,3,4-TCA), 2,4,6-tribromoanisole (2,4,6-TBA), and trans-2,cis-6-nonadienal (NDE) were investigated in 13 water supply reservoirs and 2 water treatment plants (WTPs) in S City of China...
January 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27782155/structural-elucidation-of-estrus-urinary-lipocalin-protein-eulp-and-evaluating-binding-affinity-with-pheromones-using-molecular-docking-and-fluorescence-study
#12
Durairaj Rajesh, Subramanian Muthukumar, Ganesan Saibaba, Durairaj Siva, Mohammad Abdulkader Akbarsha, Balázs Gulyás, Parasuraman Padmanabhan, Govindaraju Archunan
Transportation of pheromones bound with carrier proteins belonging to lipocalin superfamily is known to prolong chemo-signal communication between individuals belonging to the same species. Members of lipocalin family (MLF) proteins have three structurally conserved motifs for delivery of hydrophobic molecules to the specific recognizer. However, computational analyses are critically required to validate and emphasize the sequence and structural annotation of MLF. This study focused to elucidate the evolution, structural documentation, stability and binding efficiency of estrus urinary lipocalin protein (EULP) with endogenous pheromones adopting in-silico and fluorescence study...
October 26, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27649129/chemical-and-sensory-evaluation-of-silicone-and-polylactic-acid-based-remedial-treatments-for-elevated-methoxypyrazine-levels-in-wine
#13
Andreea Botezatu, Belinda S Kemp, Gary J Pickering
Alkylmethoxypyrazines (MPs) are a class of compounds that can elicit undesirable aroma and flavor characteristics in wine, and resist remediation using traditional wine making approaches. MPs are grape-derived constituents as well as contaminants from Coccinellidae beetles present during wine processing; the latter eliciting an off-flavor referred to as 'ladybug taint'. In this study we investigated the capacity of two plastic polymers-one silicone-based, the other polylactic acid-based-applied with varying surface areas to reduce concentrations of isopropylmethoxypyrazine (IPMP), sec-butylmethoxypyrazine (SBMP) and isobutylmethoxypyrazine (IBMP) in a Merlot wine using multi-dimensional gas chromatography coupled with mass spectrometry and headspace solid phase microextraction (SPME-MDGCMS)...
September 16, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27480695/solid-phase-mesh-enhanced-sorption-from-headspace-spmesh-coupled-to-dart-ms-for-rapid-quantification-of-trace-level-volatiles
#14
Jillian A Jastrzembski, Gavin L Sacks
Quantitation of trace-level (μg/L to ng/L) volatile compounds is routinely performed in a broad range of applications, including analyses of odorants, pesticide residues, or toxins in foodstuffs and related matrices. Conventional analyses based on gas chromatography-mass spectrometry (GC-MS) are limited by low throughput, and ambient approaches to sample introduction have typically had poor sensitivity. We prepared polydimethylsiloxane-coated stainless steel meshes for extraction and preconcentration of volatiles (Solid Phase Mesh Enhanced Sorption from Headspace, SPMESH), which could then be analyzed by Direct Analysis in Real Time (DART)-MS...
September 6, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27279277/phytoplasma-sap11-alters-3-isobutyl-2-methoxypyrazine-biosynthesis-in-nicotiana-benthamiana-by-suppressing-nbomt1
#15
Choon Meng Tan, Chia-Hua Li, Nai-Wen Tsao, Li-Wen Su, Yen-Ting Lu, Shu Heng Chang, Yi Yu Lin, Jyun-Cyuan Liou, Li-Ching Hsieh, Jih-Zu Yu, Chiou-Rong Sheue, Sheng-Yang Wang, Chin-Fa Lee, Jun-Yi Yang
Phytoplasmas are bacterial phytopathogens that release virulence effectors into sieve cells and act systemically to affect the physiological and morphological state of host plants to promote successful pathogenesis. We show here that transgenic Nicotiana benthamiana lines expressing the secreted effector SAP11 from Candidatus Phytoplasma mali exhibit an altered aroma phenotype. This phenomenon is correlated with defects in the development of glandular trichomes and the biosynthesis of 3-isobutyl-2-methoxypyrazine (IBMP)...
July 2016: Journal of Experimental Botany
https://www.readbyqxmd.com/read/27211692/grape-cluster-microclimate-influences-the-aroma-composition-of-sauvignon-blanc-wine
#16
Damian Martin, Claire Grose, Bruno Fedrizzi, Lily Stuart, Abby Albright, Andrew McLachlan
New Zealand Sauvignon blanc (SB) wines are characterised by a distinctive combination of tropical-fruity and green-herbaceous aromatic compounds. The influence of sunlight exposure of grape clusters on juice and wine composition was investigated, with the aim of manipulating aromatic compounds in SB wine. In the absence of basal leaf removal SB clusters naturally exposed to sunlight were riper than shaded clusters, evidenced by higher total soluble solids (TSS) and proline, and lower malic acid, 3-isobutyl-2-methoxypyrazine (IBMP) and arginine...
November 1, 2016: Food Chemistry
https://www.readbyqxmd.com/read/27180819/impact-of-leaf-removal-applied-before-and-after-flowering-on-anthocyanin-tannin-and-methoxypyrazine-concentrations-in-merlot-vitis-vinifera-l-grapes-and-wines
#17
Paolo Sivilotti, Jose Carlos Herrera, Klemen Lisjak, Helena Baša Česnik, Paolo Sabbatini, Enrico Peterlunger, Simone Diego Castellarin
The development and accumulation of secondary metabolites in grapes determine wine color, taste, and aroma. This study aimed to investigate the effect of leaf removal before flowering, a practice recently introduced to reduce cluster compactness and Botrytis rot, on anthocyanin, tannin, and methoxypyrazine concentrations in 'Merlot' grapes and wines. Leaf removal before flowering was compared with leaf removal after flowering and an untreated control. No effects on tannin and anthocyanin concentrations in grapes were observed...
June 8, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/26653458/a-comparison-of-sorptive-extraction-techniques-coupled-to-a-new-quantitative-sensitive-high-throughput-gc-ms-ms-method-for-methoxypyrazine-analysis-in-wine
#18
COMPARATIVE STUDY
Anna K Hjelmeland, Philip L Wylie, Susan E Ebeler
Methoxypyrazines are volatile compounds found in plants, microbes, and insects that have potent vegetal and earthy aromas. With sensory detection thresholds in the low ng L(-1) range, modest concentrations of these compounds can profoundly impact the aroma quality of foods and beverages, and high levels can lead to consumer rejection. The wine industry routinely analyzes the most prevalent methoxypyrazine, 2-isobutyl-3-methoxypyrazine (IBMP), to aid in harvest decisions, since concentrations decrease during berry ripening...
February 1, 2016: Talanta
https://www.readbyqxmd.com/read/26580625/manipulation-of-superparamagnetic-beads-on-patterned-exchange-bias-layer-systems-for-biosensing-applications
#19
REVIEW
Arno Ehresmann, Iris Koch, Dennis Holzinger
A technology platform based on a remotely controlled and stepwise transport of an array arrangement of superparamagnetic beads (SPB) for efficient molecular uptake, delivery and accumulation in the context of highly specific and sensitive analyte molecule detection for the application in lab-on-a-chip devices is presented. The near-surface transport of SPBs is realized via the dynamic transformation of the SPBs' magnetic potential energy landscape above a magnetically stripe patterned Exchange-Bias (EB) thin film layer systems due to the application of sub-mT external magnetic field pulses...
November 13, 2015: Sensors
https://www.readbyqxmd.com/read/26478224/vine-nitrogen-status-does-not-have-a-direct-impact-on-2-methoxy-3-isobutylpyrazine-in-grape-berries-and-wines
#20
Pierre Helwi, Aude Habran, Sabine Guillaumie, Cécile Thibon, Ghislaine Hilbert, Eric Gomes, Serge Delrot, Philippe Darriet, Cornelis van Leeuwen
Methoxypyrazines (MP) constitute a large family of compounds that contribute to the vegetative varietal aroma of many grapevine varieties and wines. The berry content in 2-methoxy-3-isobutylpyrazine (IBMP), a major MP reminiscent of green-pepper aroma, can be influenced by environmental factors or cultural practices such as water status or mineral nutrition. To date, no study has investigated a possible direct effect of nitrogen (N) on IBMP synthesis without possible interference from water status and vigor variations...
November 11, 2015: Journal of Agricultural and Food Chemistry
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