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Ian B Hogue, J Patrick Card, Linda Rinaman, Halina Staniszewska Goraczniak, Lynn W Enquist
BACKGROUND: Viral transneuronal tracing has become a well established technology used to define the synaptic architecture of polysynaptic neural networks. NEW METHOD: In this report we define the neuroinvasive profile and reporter expression of a new recombinant of the Bartha strain of pseudorabies virus (PRV). The new recombinant, PRV-290, expresses the mTurquoise2 fluorophor and is designed to complement other isogenic recombinants of Bartha that express different reporters of infection...
August 8, 2018: Journal of Neuroscience Methods
Chao Ye, Jing Chen, Tao Wang, Jingjing Xu, Hao Zheng, Jiqiang Wu, Guoxin Li, Zhiqing Yu, Wu Tong, Xuefei Cheng, Shasha Zhou, Guangzhi Tong
BACKGROUND: The alphaherpesvirus virion host shutoff (vhs) gene, UL41, can induce degradation of host mRNAs and shut off host protein synthesis. The roles of vhs in HSV-1 and HSV-2 have been studied extensively in previous studies, however, relatively little is known about the vhs protein of PRV. METHODS: A novel method combining CRISPR/Cas9 and Gibson assembly was developed to generate UL41 null PRV variant. The properties of UL41 null PRV in vitro and in vivo were further characterized...
August 2, 2018: Virology Journal
Michael A Thornton, Manan D Mehta, Tyler T Morad, Kaitlin L Ingraham, Rana R Khankan, Khris G Griffis, Anthony K Yeung, Hui Zhong, Roland R Roy, V Reggie Edgerton, Patricia E Phelps
Olfactory ensheathing cells (OECs) are unique glia that support axon outgrowth in the olfactory system, and when used as cellular therapy after spinal cord injury, improve recovery and axon regeneration. Here we assessed the effects of combining OEC transplantation with another promising therapy, epidural electrical stimulation during a rehabilitative motor task. Sprague-Dawley rats received a mid-thoracic transection and transplantation of OECs or fibroblasts (FBs) followed by lumbar stimulation while climbing an inclined grid...
July 26, 2018: Experimental Neurology
Jemin Ahn, Tatiana M Saltos, Veronica J Tom, Shaoping Hou
AIMS: This study was designed to determine specific cell groups of the raphe nuclei (RN) that give rise to supraspinal serotonergic projections regulating the bladder reflex. METHODS: Anesthetized rats underwent surgery to open the abdomen and expose the bladder. A total of 6 µL transsynaptic neuronal tracer pseudorabies virus (PRV-152), encoding for green fluorescent protein (GFP), was injected into the bladder detrusor. After 72 or 96 h, animals were perfused and the brain was dissected for processing transverse and sagittal sections...
July 12, 2018: Neurourology and Urodynamics
Alberto Hernando, M Pelaez, Maria Teresa Lozano Albalate, Montserrat Aiger, David Izquierdo, Alberto Sanchez, Maria Isabel Lopez-Jurado, Juan Ignacio Moura, Joaquin Fidalgo, Jesus Lazaro, Eduardo Gil
The main aim of this work was to characterise the Autonomic Nervous System (ANS) response in hyperbaric environments using electrocardiogram (ECG) and pulse-photoplethysmogram (PPG) signals. To that end, 26 subjects were introduced into a hyperbaric chamber and five stages with different atmospheric pressures (1 atm; descent to 3 and 5 atm; ascent to 3 and 1 atm) were recorded. Respiratory information was extracted from the ECG and PPG signals and a combined respiratory rate was studied. This information was also used to analyse Heart Rate Variability (HRV) and Pulse Rate Variability (PRV)...
January 25, 2018: IEEE Journal of Biomedical and Health Informatics
Jee Soo Park, Mei Hua Jin, Chang Hee Hong
PURPOSE: The neurological molecular mechanisms underlying the voiding dysfunction associated with nonbacterial chronic prostatitis/chronic pelvic pain syndrome remain poorly understood. In this study, we assessed whether prostate inflammation activated bladder afferent neurons, leading to bladder dysfunction, and sought to elucidate the underlying mechanisms. METHODS: Thirty male Sprague-Dawley rats were divided into 3 groups: sham-saline, formalin-injected, and capsaicin-pretreated and formalin-injected...
June 2018: International Neurourology Journal
Daniel J McDuff, Ethan B Blackford, Justin R Estepp
Remote camera-based measurement of physiology has great potential for healthcare and affective computing. Recent advances in computer vision and signal processing have enabled photoplethysmography (PPG) measurement using commercially available cameras. However, there remain challenges in recovering accurate noncontact PPG measurements in the presence of rigid head motion. When a subject is moving, their face may be turned away from one camera, be obscured by an object, or move out of the frame resulting in missing observations...
August 2018: IEEE Transactions on Bio-medical Engineering
Zhixin Lei, Qianying Liu, Qianqian Zhu, Bing Yang, Haseeb Khaliq, Ao Sun, Yi Qi, Gopi Krishna Moku, Yafan Su, Jiawei Wang, Jiyue Cao, Qigai He
Antimicrobial peptide (Piscidin-1) is an effective natural polypeptide, which has great influence and potential on porcine epidemic diarrhea virus (PEDV) and pseudorabies virus (PRV). As an alternative antibiotic substitute, Piscidin-1 was subjected for pharmacokinetics study with three administration routes (i.v, i.m, and p.o) after a single dose of 2 mg/kg in rats and preliminary pharmacodynamics including antiviral activity in cell against PEDV and PRV. Based on 50 percent tissue culture infective dose (TCID50 ), there were about 2 and 10% virus survived ratios for Piscidin-1 against PRV and PEDV, respectively...
2018: Frontiers in Chemistry
Lijun Ma, Lei Wang, Young Lee, Chia-Lin Tseng, Scott Soltys, Steve Braunstein, Arjun Sahgal
Purpose: Doses to small spinal cord isodose volume (such as those ranging from Dmax 0.0 cc to 0.5 cc) as well as to large volumes (such as those ranging from 0.5 cc to 3.0 cc) are critical parameters to guide safe practice of spine SBRT. We here report a mathematical formula that links the most probable dose volume limits together for common spine SBRT cases.Methods and materials: A dose ripple formula parameterized with equivalent dose radius (EDR) was derived to model spinal cord small-volume doses for a spine SBRT treatment...
2018: Journal of Radiosurgery and SBRT
Xiang Guan, Jie Liu, Hui Jiang, Chang-Xian Wu, Huan-Chun Chen, Zheng-Fei Liu
Long noncoding RNAs (lncRNAs) play important roles in regulating eukaryotic genome replication and gene expression in diverse biological systems. Here, we identified lncRNAs transcribed from pseudorabies virus (PRV)-infected PK-15 cells. Based on high-throughput sequencing data, we obtained 87,263,926 and 93,947,628 clean reads from mock-infected and PRV-infected PK-15 cells, respectively. Through a normalized analytic protocol, we identified three novel viral lncRNAs. According to an analysis of differential expression between the mock-infected and PRV-infected cells, 4151 host lncRNAs were significantly upregulated and 2327 host lncRNAs were significantly downregulated in the latter group...
July 9, 2018: Journal of Neurovirology
M V Talamini Junior, A C S de Araujo, A P de Camargo, E Saretta, J A Frizzone
The functionality of pressure regulating valves (PRVs) is important for proper uniformity and efficiency of irrigation during center pivot irrigation, especially when the center pivot operates on sloping terrain. In practice, the regulated pressure at the PRV outlet is slightly influenced by its inlet pressure, the flow rate through it, and hysteresis effects. The objectives of this work were (a) to evaluate operational characteristics of PRVs based on requirements stated by ISO 10522 (1993) and (b) to model the regulated pressure as a function of inlet pressure and flow rate through the valve considering hysteresis...
2018: TheScientificWorldJournal
N G Burnet, D J Noble, A Paul, G A Whitfield, S Delorme
CLINICAL ISSUE: Successful radiotherapy requires precise localization of the tumor and requires high-quality imaging for developing a treatment plan. STANDARD TREATMENT: Irradiation of the tumor region, including a safety margin. TREATMENT INNOVATIONS: The target volume consists of the gross tumor volume (GTV) containing visible parts of the tumor, the clinical target volume (CTV) covering the GTV plus invisible tumor extensions, and the planning target volume (PTV) to account for uncertainties...
June 27, 2018: Der Radiologe
Øystein Wessel, Øyvind Haugland, Marit Rode, Børge N Fredriksen, Maria K Dahle, Espen Rimstad
Heart- and skeletal muscle inflammation (HSMI) caused by infection with Piscine orthoreovirus (PRV) is one of the most common viral diseases in farmed Atlantic salmon (Salmo salar) in Norway, and disease outbreaks have been reported in most countries with large-scale Atlantic salmon aquaculture. Currently there is no vaccine available for protection against HSMI, partly due to the lack of a cell line for efficient virus propagation. Erythrocytes are the primary target cells for PRV in vivo and a potential source for isolation of PRV particles...
June 21, 2018: Journal of Fish Diseases
Dóra Tombácz, Donald Sharon, Attila Szűcs, Norbert Moldován, Michael Snyder, Zsolt Boldogkői
Pseudorabies virus (PRV) is an alphaherpesvirus of swine. PRV has a large double-stranded DNA genome and, as the latest investigations have revealed, a very complex transcriptome. Here, we present a large RNA-Seq dataset, derived from both short- and long-read sequencing. The dataset contains 1.3 million 100 bp paired-end reads that were obtained from the Illumina random-primed libraries, as well as 10 million 50 bp single-end reads generated by the Illumina polyA-seq. The Pacific Biosciences RSII non-amplified method yielded 57,021 reads of inserts (ROIs) aligned to the viral genome, the amplified method resulted in 158,396 PRV-specific ROIs, while we obtained 12,555 ROIs using the Sequel platform...
June 19, 2018: Scientific Data
Tao Wang, Wu Tong, Chao Ye, Zhiqing Yu, Jing Chen, Fei Gao, Tongling Shan, Hai Yu, Liwei Li, Guoxin Li, Guangzhi Tong, Hao Zheng
Since late 2011, a pseudorabies virus (PRV) variant with increased virulence in old pigs had caused major disease outbreaks and great economic losses to the pig industry in China. The gene mutations that contributed to the increased virulence were unclear. To study the basis of the enhanced pathogenicity, an infectious bacterial artificial chromosome (BAC) clone consisting of the complete genome of the PRV variant was developed. Using homologous recombination and Cre/LoxP recombination, the recombinant virus rJS-2012-BAC carrying a BAC insertion downstream of the open reading frame (ORF) of gG was constructed...
September 2018: Journal of Virological Methods
Åse Helen Garseth, Eirik Biering
No abstract text is available yet for this article.
June 11, 2018: Journal of Fish Diseases
Zhe Chen, Lejia Sun, Hui Chen, Dachuan Gu, Weitao Zhang, Zifeng Yang, Tao Peng, Rong Dong, Kefang Lai
Neurogenic airway inflammation in chronic cough and bronchial asthma related to gastroesophageal reflux (GER) is involved in the esophageal-bronchial reflex, but it is unclear whether this reflex is mediated by central neurons. This study aimed to investigate the regulatory effects of the dorsal vagal complex (DVC) on airway inflammation induced by the esophageal perfusion of hydrochloric acid (HCl) following the microinjection of nuclei in the DVC in guinea pigs. Airway inflammation was evaluated by measuring the extravasation of Evans blue dye (EBD) and substance P (SP) expression in the airway...
2018: Frontiers in Physiology
Xialin Zuo, Qinghua Hou, Jizi Jin, Xiaohui Chen, Lixuan Zhan, Yanyan Tang, Zhe Shi, Weiwen Sun, En Xu
Stroke is the leading cause of adult disability in the world. In general, recovery from stroke is incomplete. Accumulating evidences have shown that focal cerebral infarction leads to dynamic trans-neuronal degeneration in non-ischemic remote brain regions, with the disruption of connections to synapsed neurons sustaining ischemic insults. Previously, we had reported that the ipsilateral striatum, thalamus degenerated in succession after permanent distal branch of middle cerebral artery occlusion (dMCAO) in Sprague-Dawley (SD) rats and cathepsin (Cath) B was activated before these relay degeneration...
2018: Frontiers in Aging Neuroscience
Genxuan Zhang, Bo Shi, Sai Zhang, Young Tsau
In this paper, based on the feature of ensemble empirical mode decomposition(EEMD) which is very suitable for analyzing non-stationary and nonlinear signals, the pulse rate variability (PRV) signal is decomposed into multiple intrinsic mode function (IMF) components by EEMD, to calculate the energy of each IMF component respectively, and high-frequency energy(EHF), low-frequency energy(ELF) and very low-frequency energy(EVLF) of PRV were reconstructed from the energy of the IMF components according to the characteristics of each IMF component spectrum...
July 30, 2017: Zhongguo Yi Liao Qi Xie za Zhi, Chinese Journal of Medical Instrumentation
Andrius Rapalis, Andrius Petrėnas, Monika Šimaitytė, Raquel Bailón, Vaidotas Marozas
OBJECTIVE: The growing interest to integrate consumer smart wristbands in eHealth applications spawns the need for novel approaches of data parametrization which account for the technology-specific constraints. The present study aims to investigate the feasibility of a consumer smart wristband to be used for computing pulse rate parameters during free-living activities. APPROACH: The feasibility of computing pulse rate variability (PRV) as well as pulse rate and physical activity-related parameters using the smart wristband was investigated, having an electrocardiogram as a reference...
May 31, 2018: Physiological Measurement
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