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https://www.readbyqxmd.com/read/29679563/shrna-mediated-knock-down-of-cxcl8-inhibits-tumor-growth-in-colorectal-liver-metastasis
#1
Abhishek Kumar, Madhuri Cherukumilli, Seyed Hamidreza Mahmoudpour, Karsten Brand, Obul Reddy Bandapalli
CXCL8 belongs to proinflammatory chemokines that are predominantly involved in neutrophil chemotaxis and degranulation. Several studies have suggested that secretion of CXCL8 from cancer cells have a profound effect on tumor microenvironment. In this study, in continuation to our previous work of understanding the global picture of invasion related genes in colorectal liver metastases, we clearly show an up-regulation of CXCL8 expression in the tumor cells at the invasion front as compared to the tumor cells in the inner parts of the tumor...
April 18, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29678882/mesenchyme-homeobox-1-mediates-transforming-growth-factor-%C3%AE-tgf-%C3%AE-induced-smooth-muscle-cell-differentiation-from-mouse-mesenchymal-progenitors
#2
Kun Dong, Xia Guo, Weiping Chen, Amanda C Hsu, Qiang Shao, Jian-Fu Chen, Shi-You Chen
Differentiation of smooth muscle cells (SMCs) is critical for proper vasculogenesis and angiogenesis. However, the molecular mechanisms controlling SMC differentiation are not completely understood. During embryogenesis, the transcription factor mesenchyme homeobox 1 (Meox1) is expressed in the early developing somite, which is one of the origins of SMCs. In the present study, we identified Meox1 as a positive regulator of SMC differentiation. We found that transforming growth factor-β (TGF-β) induces Meox1 expression in the initial phase of SMC differentiation of pluripotent murine C3H10T1/2 cells...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29678744/long-noncoding-rna-lncarsr-promotes-hepatic-cholesterol-biosynthesis-via-modulating-akt-srebp-2-hmgcr-pathway
#3
Jiabin Hang, Shangjun Chen, Dongliang Cai, Deqiang Bian, Fengling Wang
AIMS: Disruption of cholesterol homeostasis has been identified as a major factor in the pathogenesis of atherosclerosis, myocardial infarction, and strokes. Long noncoding RNAs (lncRNAs) have emerged as critical players in cellular cholesterol metabolism, but their functions are still largely unknown. MATERIALS AND METHODS: C57BL6/j mice were fed with high cholesterol diet (containing 4% cholesterol) or chow diet. Adenoviruses-lncARSR and lncARSR shRNA were used to overexpress or knockdown lncARSR expression...
April 17, 2018: Life Sciences
https://www.readbyqxmd.com/read/29678571/the-transcription-factor-mef2a-plays-a-key-role-in-the-differentiation-maturation-of-rat-neural-stem-cells-into-neurons
#4
Bangfu Zhu, Ruth E Carmichael, Luis Solabre Valois, Kevin A Wilkinson, Jeremy M Henley
Neural stem cells (NSCs) are self-renewing multipotent stem cells that can be proliferated in vitro and differentiated into neuronal and/or glial lineages, making them an ideal model to study the processes involved in neuronal differentiation. Here we have used NSCs to investigate the role of the transcription factor MEF2A in neuronal differentiation and development in vitro. We show that although MEF2A is present in undifferentiated NSCs, following differentiation it is expressed at significantly higher levels in a subset of neuronal compared to non-neuronal cells...
April 17, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29677029/kindlin-1-regulates-astrocyte-activation-and-pain-sensitivity-in-rats-with-neuropathic-pain
#5
Baisong Zhao, Yongying Pan, Haiping Xu, Xingrong Song
BACKGROUND AND OBJECTIVES: Astrocyte activation has been implicated in the pathogenesis of neuropathic pain, but the involvement of kindlin-1 in astrocyte activation and neuropathic pain has not yet been illustrated. Using a chronic constriction injury (CCI) rat model of neuropathic pain, we investigated the expression levels of kindlin-1 during neuropathic pain and the influences of kindlin-1 on regulating pain sensitivity. METHODS: Neuropathic pain was induced in rats by CCI of the sciatic nerve...
April 19, 2018: Regional Anesthesia and Pain Medicine
https://www.readbyqxmd.com/read/29675777/inhibition-of-p16-ink4a-to-rejuvenate-aging-human-cardiac-progenitor-cells-via-the-upregulation-of-anti-oxidant-and-nf%C3%AE%C2%BAb-signal-pathways
#6
Roshni V Khatiwala, Shuning Zhang, Xiuchun Li, Neil Devejian, Edward Bennett, Chuanxi Cai
Autologous human cardiac stem/progenitor cell (hCPC) therapy is a promising treatment that has come into use in recent years for patients with cardiomyopathy. Though innovative in theory, a major hindrance to the practical application of this treatment is that the hCPCs of elderly patients, who are most susceptible to myocardial disease, are senescent and prone to cell death. Rejuvenating hCPCs from elderly patients may help overcome this obstacle, and can be accomplished by reversing entry into the cellular stage of senescence...
April 19, 2018: Stem Cell Reviews
https://www.readbyqxmd.com/read/29675113/downregulation-of-bancr-promotes-aggressiveness-in-papillary-thyroid-cancer-via-the-mapk-and-pi3k-pathways
#7
Jinjun Zhang, Yaying Du, Xiaoxue Zhang, Mengchen Li, Xingrui Li
Recent evidence indicates that long non-coding RNAs play important roles in tumorigenesis and cancer progression. BRAF -activated non-protein coding RNA ( BANCR ) is a novel and potential regulator of cancer cell proliferation and migration. However, little is known regarding the role of BANCR in papillary thyroid cancer (PTC). The current study used quantitative PCR to demonstrate that BANCR was significantly downregulated in 60 paired PTC tissues compared with normal tissues. In addition, BANCR was significantly correlated with lymph node metastasis ( p = 0...
2018: Journal of Cancer
https://www.readbyqxmd.com/read/29674640/the-cpt1a-inhibitor-etomoxir-induces-severe-oxidative-stress-at-commonly-used-concentrations
#8
Roddy S O'Connor, Lili Guo, Saba Ghassemi, Nathaniel W Snyder, Andrew J Worth, Liwei Weng, Yoonseok Kam, Benjamin Philipson, Sophie Trefely, Selene Nunez-Cruz, Ian A Blair, Carl H June, Michael C Milone
Etomoxir (ETO) is a widely used small-molecule inhibitor of fatty acid oxidation (FAO) through its irreversible inhibitory effects on the carnitine palmitoyl-transferase 1a (CPT1a). We used this compound to evaluate the role of fatty acid oxidation in rapidly proliferating T cells following costimulation through the CD28 receptor. We show that ETO has a moderate effect on T cell proliferation with no observable effect on memory differentiation, but a marked effect on oxidative metabolism. We show that this oxidative metabolism is primarily dependent upon glutamine rather than FAO...
April 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29674524/insulin-receptor-plasma-membrane-levels-increased-by-the-progesterone-receptor-membrane-component-1
#9
Kaia K Hampton, Hilaree Frazier, Katie Anderson, Olivier Thibault, Rolf J Craven
The Insulin Receptor (IR) is a ligand-activated receptor tyrosine kinase that has a key role in metabolism, cellular survival and proliferation. Progesterone receptor membrane component 1 (PGRMC1) promotes cellular signaling via receptor trafficking, and is essential for some elements of tumor growth and metastasis. In the present study, we demonstrate that PGRMC1 co-precipitates with IR. Furthermore, we show that PGRMC1 increases plasma membrane IR levels in multiple cell lines and decreases insulin binding at the cell surface...
April 19, 2018: Molecular Pharmacology
https://www.readbyqxmd.com/read/29673706/lysophosphatidylcholine-acyltransferase-3-deficiency-impairs-3t3l1-cell-adipogenesis-through-activating-wnt-%C3%AE-catenin-pathway
#10
Chunyan Feng, Bin Lou, Jibin Dong, Zhiqiang Li, Yunqin Chen, Yue Li, Xuemei Zhang, Xian-Cheng Jiang, Tingbo Ding
Levels of polyunsaturated phosphatidylcholine (PC) influence plasma membrane structure and function. Phosphatidylcholine (PC) is synthesized de novo in the Kennedy pathway and then undergoes extensive deacylation/reacylation remodeling via Lands' cycle (non-Kennedy pathway). The reacylation is catalyzed by lysophosphatidylcholine acyltransferase (LPCAT), which adds a polyunsaturated fatty acid at the sn-2 position. Four LPCAT isoforms have been described to date, among which we found LPCAT3 to be the major isoform in adipose tissue, but its exact role in adipogenesis is unclear...
April 16, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29673453/-knockdown-of-stat3-inhibits-proliferation-and-migration-of-hepg2-hepatoma-cells-induced-by-ifn1
#11
Xiaofang Li, Yuqi Wang, Ben Yan, Peipei Fang, Chao Ma, Ning Xu, Xiaoyan Fu, Shujuan Liang
Objective To prepare lentiviruses expressing shRNA sequences targeting human signal transducer and activator of transcription 3 (STAT3) and detect the effect of STAT3 knockdown on type I interferon (IFN1)-induced proliferation and migration in HepG2 cells. Methods Four STAT3-targeting shRNA sequences (shRNA1-shRNA4) and one control sequence (Ctrl shRNA) were selected and cloned respectively into pLKO.1-sp6-pgk-GFP to construct shRNA-expressing vectors. Along with backbone psPAX2 and pMD2.G vectors, they were separately transfected into HEK293T cells to prepare lentiviruses...
February 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29673358/contribution-of-tlr4-signaling-in-intermittent-hypoxia-mediated-atherosclerosis-progression
#12
Xianqin Zeng, Rong Guo, Mei Dong, Julia Zheng, Huili Lin, Huixia Lu
BACKGROUND: Intermittent hypoxia (IH), a typical character of obstructive sleep apnea (OSA), is related to atherogenesis. However, the role of IH on atherosclerosis (AS) progression and the mechanisms involved remains poorly understood. METHODS: In the present study, high-fat fed ApoE-/- mice were treated with recombinant shRNA-TLR4 lentivirus and exposed to IH. Atherosclerotic lesions on the en face aorta and cross-sections of aortic root were examined by Oil-Red O staining...
April 19, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29672920/targeting-hsp60-by-subcutaneous-injections-of-jetpei-hsp60-shrna-destabilizes-cytoplasmic-survivin-and-inhibits-hepatocellular-carcinoma-growth
#13
Ya-Hui Huang, Kwang-Huei Lin, Jao-Song Yu, Ting-Jung Wu, Wei-Chen Lee, Chuck C-K Chao, Tai-Long Pan, Chau-Ting Yeh
Heat shock protein 60 (HSP60) overexpresses in various types of cancer, but its expression levels and functions in hepatocellular carcinoma (HCC) are still in dispute. We aim to clarify this issue and examine whether HSP60 could be a therapeutic target for HCC. We found drastically enhanced cell apoptosis and suppressed cell proliferation in two HCC cell lines with HSP60-silencing, and also indicated survivin is involved in this regulatory process in vitro and in vivo. However, HSP60-silencing in normal human hepatocytes only resulted in a minimal reduction of cell proliferation but without effects on cell apoptosis...
April 19, 2018: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29671263/high-throughput-screening-method-to-identify-alternative-splicing-regulators
#14
Cheryl Stork, Sika Zheng
Misregulation of alternative pre-mRNA splicing contributes to various diseases. Understanding how alternative splicing is regulated paves the way to modulating or correcting molecular pathogenesis of the diseases. Alternative splicing is typically regulated by trans RNA binding proteins and their upstream modulators. Identification of these splicing regulators has been difficult and traditionally done piecemeal. High-throughput screening strategies to find multiple regulators of exon splicing have great potential to accelerate the discovery process, but typically confront low sensitivity and specificity of splicing assays...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29671197/distinct-response-to-gdf15-knockdown-in-pediatric-and-adult-glioblastoma-cell-lines
#15
Mirella Baroni, Suely Kazue Nagahashi Marie, Paola Fernanda Fedatto, Augusto Faria Andrade, Veridiana Kill Suazo, Gustavo Alencastro Veiga Cruzeiro, Rosane de Paula Queiroz, Luiz Gonzaga Tone, Carlos Alberto Scrideli
INTRODUCTION: Glioblastoma (GBM) is the most common malignant primary brain tumor affecting adults. In pediatric patients, GBM exhibits genetic variations distinct from those identified in the adult GBM phenotype. This tumor exhibits complex genetic changes leading to malignant progression and resistance to standard therapies including radiotherapy and temozolomide treatment. The GDF15 gene codes for a growth factor whose expression is altered in the presence of inflammations and malignancies...
April 18, 2018: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/29670371/knockdown-of-fibrous-sheath-interacting-protein-1-expression-reduces-bladder-urothelial-carcinoma-cell-proliferation-and-induces-apoptosis-via-inhibition-of-the-pi3k-akt-pathway
#16
Ming Sun, Zhaofu Chen, Shutao Tan, Caigang Liu, Wenyan Zhao
Background: FSIP1 plays a vital role in tumorigenesis and cancer progression. In bladder cancer, FSIP1 overexpression was associated with poor prognosis of bladder urothelial carcinoma. In this study, we investigated whether FSIP1 is essential in the progression of bladder cancer and the mechanism by which it mediates this effect. Methods: FSIP1 expression was knocked down in bladder cancer cells using lentiviral-mediated short hairpin RNA (shRNA). FSIP1 expression was detected using Western blotting, immunohistochemistry (IHC), and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR)...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29669943/whole-exome-sequencing-uncovers-oxidoreductases-dhtkd1-and-ogdhl-as-linkers-between-mitochondrial-dysfunction-and-eosinophilic-esophagitis
#17
Joseph D Sherrill, Kiran Kc, Xinjian Wang, Ting Wen, Adam Chamberlin, Emily M Stucke, Margaret H Collins, J Pablo Abonia, Yanyan Peng, Qiang Wu, Philip E Putnam, Phillip J Dexheimer, Bruce J Aronow, Leah C Kottyan, Kenneth M Kaufman, John B Harley, Taosheng Huang, Marc E Rothenberg
Eosinophilic esophagitis (EoE) is an allergic inflammatory esophageal disorder with a complex underlying genetic etiology often associated with other comorbidities. Using whole-exome sequencing (WES) of 63 patients with EoE and 60 unaffected family members and family-based trio analysis, we sought to uncover rare coding variants. WES analysis identified 5 rare, damaging variants in dehydrogenase E1 and transketolase domain-containing 1 (DHTKD1). Rare variant burden analysis revealed an overabundance of putative, potentially damaging DHTKD1 mutations in EoE (P = 0...
April 19, 2018: JCI Insight
https://www.readbyqxmd.com/read/29669300/gzmb-gene-silencing-confers-protection-against-synovial-tissue-hyperplasia-and-articular-cartilage-tissue-injury-in-rheumatoid-arthritis-through-the-mapk-signaling-pathway
#18
Cui-Xia Bao, Hai-Xia Chen, Xue-Jie Mou, Xiang-Kui Zhu, Qi Zhao, Xin-Guang Wang
INTRODUCTION: Rheumatoid arthritis (RA) represents the most commonly occurring inflammatory type of arthritis and is a major cause of disability. Reports have placed emphasis on the potential of, granzyme B (GZMB) as a potentially valuable prognostic marker in early RA, the mechanism of which still remains largely unclear. Thus, the aim of the current study was to investigate the effects GZMB gene silencing influences synovial tissue hyperplasia and articular cartilage tissue injury of RA through the regulation of the MAPK signaling pathway...
April 15, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29668357/formin-dependent-tgf-%C3%AE-signaling-for-epithelial-to-mesenchymal-transition
#19
Manish K Rana, Francesca M Aloisio, Changhoon Choi, Diane L Barber
The role of distinct actin filaments architectures in epithelial plasticity remains incompletely understood. We, therefore, determined roles for formins and the Arp2/3 complex, which are actin nucleators generating unbranched and branched actin filaments, respectively, in the process of epithelial to mesenchymal transition (EMT). In clonal lung, mammary and renal epithelial cells, the formin activity inhibitor SMIFH2 but not the Arp2/3 complex activity inhibitor CK666 blocked EMT induced by TGF-β. SMIFH2 prevented the proximal signal of increased Smad2 phosphorylation, and hence also blocked downstream EMT markers, including actin filament remodeling, decreased expression of the adherens junction protein E-cadherin and increased expression of the matrix protein fibronectin and the transcription factor Snail...
April 18, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29667751/suz12-is-a-novel-putative-oncogene-promoting-tumorigenesis-in-head-and-neck-squamous-cell-carcinoma
#20
Yaping Wu, Huijun Hu, Wei Zhang, Zhongwu Li, Pengfei Diao, Dongmiao Wang, Wei Zhang, Yanling Wang, Jianrong Yang, Jie Cheng
The suppressor of zest 12 (SUZ12), one of the core polycomb repressive complex 2 (PRC2) components, has increasingly appreciated as a key mediator during human tumorigenesis. However, its expression pattern and oncogenic roles in head and neck squamous cell carcinoma (HNSCC) remain largely unexplored yet. Here, we sought to determine its expression pattern, clinicopathological significance and biological roles in HNSCC. Through data mining and interrogation from multiple publicly available databases, our bioinformatics analyses revealed that SUZ12 mRNA was significantly overexpressed in multiple HNSCC patient cohorts...
April 18, 2018: Journal of Cellular and Molecular Medicine
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