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https://www.readbyqxmd.com/read/29458173/deregulation-of-nrf2-are-signaling-pathway-causes-susceptibility-of-dystrophin-deficient-myotubes-to-menadione-induced-oxidative-stress
#1
Su Jin Choi, Hye Sun Kim
Duchenne muscular dystrophy (DMD) is an X chromosome-linked disorder caused by a mutation in the dystrophin gene. Many previous studies reported that the skeletal muscles of DMD patients were more susceptible to oxidative stress than those of healthy people. However, not much has been known about the responsible mechanism of the differential susceptibility. In this study, we established dystrophin knock-down (DysKD) cell lines by transfection of dystrophin shRNA lentiviral particles into C2 cells and found that DysKD myotubes are more vulnerable to menadione-induced oxidative stress than control myotubes...
February 16, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29458011/inflammatory-cytokines-induce-podoplanin-expression-at-the-tumor-invasive-front
#2
Akiko Kunita, Vanessa Baeriswyl, Claudia Meda, Erik Cabuy, Kimiko Takeshita, Enrico Giraudo, Andreas Wicki, Masashi Fukayama, Gerhard Christofori
Tumor invasion is a critical first step in the organismic dissemination of cancer cells and the formation of metastasis in distant organs, the most important prognostic factor and the actual cause of death in most of the cancer patients. We report here that the cell surface protein podoplanin (PDPN), a potent inducer of cancer cell invasion, is conspicuously expressed by the invasive front of squamous cell carcinomas (SCC) of the cervix in patients and in the transgenic HPV/E2 mouse model of cervical cancer...
February 16, 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29455656/the-e3-ubiquitin-ligase-nedd4-mediates-cell-migration-signaling-of-egfr-in-lung-cancer-cells
#3
Genbao Shao, Ranran Wang, Aiqin Sun, Jing Wei, Ke Peng, Qian Dai, Wannian Yang, Qiong Lin
BACKGROUND: EGFR-dependent cell migration plays an important role in lung cancer progression. Our previous study observed that the HECT E3 ubiquitin ligase NEDD4 is significantly correlated with tumor metastasis and required for migration and invasion signaling of EGFR in gastric cancer cells. However, how NEDD4 promotes the EGFR-dependent lung cancer cell migration is unknown. This study is to elucidate the mechanism by which NEDD4 mediates the EGFR lung cancer migration signaling. METHODS: Lentiviral vector-loaded NEDD4 shRNA was used to deplete endogenous NEDD4 in lung cancer cell lines...
February 19, 2018: Molecular Cancer
https://www.readbyqxmd.com/read/29455446/big2-arf1-rhoa-mdia1-signaling-regulates-dendritic-golgi-polarization-in-hippocampal-neurons
#4
Eun-Hye Hong, Ji-Ye Kim, Jeong-Hoon Kim, Dae-Sik Lim, Minkyu Kim, Jeong-Yoon Kim
Proper dendrite development is essential for establishing neural circuitry, and Rho GTPases play key regulatory roles in this process. From mouse brain lysates, we identified Brefeldin A-inhibited guanine exchange factor 2 (BIG2) as a novel Rho GTPase regulatory protein involved in dendrite growth and maintenance. BIG2 was highly expressed during early development, and knockdown of the ARFGEF2 gene encoding BIG2 significantly reduced total dendrite length and the number of branches. Expression of the constitutively active ADP-ribosylation factor 1 ARF1 Q71L rescued the defective dendrite morphogenesis of ARFGEF2-null neurons, indicating that BIG2 controls dendrite growth and maintenance by activating ARF1...
February 17, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29455395/anti-cancer-effect-of-aquaporin-5-silencing-in-colorectal-cancer-cells-in-association-with-inhibition-of-wnt-%C3%AE-catenin-pathway
#5
Wei Wang, Qing Li, Tao Yang, Dongsheng Li, Feng Ding, Hongzhi Sun, Guang Bai
Aquaporin 5 (AQP5) is a water channel protein that is over-expressed in many tumors. Elevated expression of AQP5 is associated with poor prognosis of colorectal cancer. Yet, whether AQP5 plays a role in epithelial-mesenchymal transition (EMT) of colorectal cancer has not been reported until now. Here we aim to investigate the function of AQP5 in the EMT process of colorectal cancer. We transfected HCT116 and SW480 cells with AQP5-specific shRNA and verified the knockdown efficiency through western blotting and real-time PCR...
February 17, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29454035/high-and-low-doses-of-cocaine-intake-are-differentially-regulated-by-dopamine-d2-receptors-in-the-ventral-tegmental-area-and-the-nucleus-accumbens
#6
R Chen, S McIntosh, S E Hemby, H Sun, T Sexton, Thomas Martin, S R Childers
Dopamine D2 receptors (D2Rs) in the ventral tegmental area (VTA) and the nucleus accumbens (NAc) are associated with vulnerability to addiction; however, whether D2Rs in these two brain regions play differential roles in regulation of drug intake is unknown. Here, we compared the effect of decreased mRNA level of Drd2 in each region on cocaine self-administration in a dose-response function. Drd2 mRNA levels in rat VTA or NAc were knocked down by bilateral microinjection of lentivirus coding shRNAs against rat Drd2...
February 14, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29451218/pten-knockdown-with-the-y444f-mutant-aav2-vector-promotes-axonal-regeneration-in-the-adult-optic-nerve
#7
Zheng-Ru Huang, Hai-Ying Chen, Zi-Zhong Hu, Ping Xie, Qing-Huai Liu
The lack of axonal regeneration is the major cause of vision loss after optic nerve injury in adult mammals. Activating the PI3K/AKT/mTOR signaling pathway has been shown to enhance the intrinsic growth capacity of neurons and to facilitate axonal regeneration in the central nervous system after injury. The deletion of the mTOR negative regulator phosphatase and tensin homolog (PTEN) enhances regeneration of adult corticospinal neurons and ganglion cells. In the present study, we used a tyrosine-mutated (Y444F) AAV2 vector to efficiently express a short hairpin RNA (shRNA) for silencing PTEN expression in retinal ganglion cells...
January 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/29448842/knockdown-of-nrage-induces-odontogenic-differentiation-by-activating-nf-%C3%AE%C2%BAb-signaling-in-mouse-odontoblast-like-cells
#8
Qi Wu, Jing Li, Pingping Song, Jing Chen, Yuanzhi Xu, Shengcai Qi, Ji Ma, Qiuhui Pan
PURPOSE: Neurotrophin receptor-interacting MAGE homologue (Nrage) plays an important role in bone development and the metabolism of normal skeletal structures. Our previous study showed that Nrage inhibited the odontogenic differentiation of mouse dental pulp cells. However, the potential roles and mechanism of Nrage in regulating odontogenic differentiation are unknown. The aim of this study was to investigate the molecular mechanism of Nrage in odontogenic differentiation of mouse odontoblast-like cells...
February 15, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29448247/knockdown-of-magea6-activates-amp-activated-protein-kinase-ampk-signaling-to-inhibit-human-renal-cell-carcinoma-cells
#9
Xueting Ye, Jing Xie, Hang Huang, Zhexian Deng
BACKGROUND/AIMS: Melanoma antigen A6 (MAGEA6) is a cancer-specific ubiquitin ligase of AMP-activated protein kinase (AMPK). The current study tested MAGEA6 expression and potential function in renal cell carcinoma (RCC). METHODS: MAGEA6 and AMPK expression in human RCC tissues and RCC cells were tested by Western blotting assay and qRT-PCR assay. shRNA method was applied to knockdown MAGEA6 in human RCC cells. Cell survival and proliferation were tested by MTT assay and BrdU ELISA assay, respectively...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29448104/aim2-regulates-vascular-smooth-muscle-cell-migration-in-atherosclerosis
#10
Jinyu Pan, Lu Lu, Xuyang Wang, Dian Liu, Jingjing Tian, Hui Liu, Mingjun Zhang, Fengqin Xu, Fengshuang An
BACKGROUND: Atherosclerosis (AS) is a common pathological basis of various cardiovascular and cerebrovascular diseases. Plaque formation is initiated and triggered by vascular smooth musclecells (VSMCs) migration in vascular wall, which gradually aggravates atherosclerosis progression. Absent in melanoma 2 (AIM2), a member of HIN-200 family, plays an important role in activating inflammasome. However, the role of AIM2 in atherosclerotic plaque progression outside of the inflammasome has not yet been reported...
February 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29445125/pin1-impairs-microrna-biogenesis-by-mediating-conformation-change-of-xpo5-in-hepatocellular-carcinoma
#11
Jiao Li, Wenchen Pu, Hui-Lung Sun, Jian-Kang Zhou, Xin Fan, Yuanyuan Zheng, Juan He, Xuesha Liu, Zhichu Xia, Lunxu Liu, Yu-Quan Wei, Yong Peng
MicroRNA (miRNA) dysregulation is associated with the tumorigenesis and development of numerous human cancers. The defect in miRNA biogenesis is the main cause of miRNA dysregulation. We previously demonstrated that ERK-induced phosphorylation of XPO5 followed by peptidyl-prolyl cis/trans isomerase Pin1-mediated isomerization downregulates miRNA expression and contributes to hepatocellular carcinoma (HCC) development. However, how Pin1 precisely regulates miRNA biogenesis in HCC remains elusive. Here we reveal that Pin1 has a pivotal role in the miRNA maturation process by modulating phosphorylated Serine-Proline (pS-P) motif of XPO5 in a phosphorylation-dependent manner...
February 14, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29444935/adenylyl-cyclase-1-as-a-major-isoform-to-generate-camp-signaling-for-apoa-1-mediated-cholesterol-efflux-pathway
#12
Wanze Tang, Weilie Ma, Hang Ding, Margarita Lin, Le Xiang, Guorong Lin, Zhizhen Zhang
HDL apolipoprotein A-1-mediated cholesterol efflux pathway requires multiple cellular proteins and signal transduction pathways, including adenylyl cyclase (AC) / cAMP signaling. Due to the existence of multiple transmembrane AC isoforms, it was not known how many AC isoforms are expressed and which ones are essential for cholesterol efflux in macrophage foam cells. These questions were investigated in THP-1 macrophages in this study. QRT-PCR detected mRNAs for all nine transmembrane AC isoforms, but only the mRNA and protein of AC1 isoform were consistently upregulated by cholesterol loading and apoA-1...
February 14, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29444104/shdcr3-sensitizes-trail-resistant-hcc-cells-by-inducing-caspase-dependent-apoptosis-while-suppressing-nf-%C3%AE%C2%BAb-dependent-cflipl-expression
#13
Dong-Yu Liang, Wei Huang, Qing Chang, Yan-Qiang Hou
Evidence has shown that most hepatocellular carcinoma (HCC) cells are resistant to tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis. However, the molecular mechanisms underlying TRAIL-mediated apoptosis resistance are not well understood. In this study, we reported that downregulation of Decoy receptor 3 (DcR3) expression by lentiviral vectors carrying shRNA against DcR3 (LV-ShDcR3, shDcR3) in Huh7 both greatly enhanced TRAIL-mediated apoptosis and reduced cell proliferation capability...
2018: PloS One
https://www.readbyqxmd.com/read/29443066/constitutive-and-inducible-systems-for-genetic-in-vivo-modification-of-mouse-hepatocytes-using-hydrodynamic-tail-vein-injection
#14
Eric K Hubner, Christian Lechler, Thomas N Rösner, Birgit Kohnke-Ertel, Roland M Schmid, Ursula Ehmer
In research models of liver cancer, regeneration, inflammation, and fibrosis, flexible systems for in vivo gene expression and silencing are highly useful. Hydrodynamic tail vein injection of transposon-based constructs is an efficient method for genetic manipulation of hepatocytes in adult mice. In addition to constitutive transgene expression, this system can be used for more advanced applications, such as shRNA-mediated gene knock-down, implication of the CRISPR/Cas9 system to induce gene mutations, or inducible systems...
February 2, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29442041/the-interaction-between-atrip-and-mcm-complex-is-essential-for-atrip-chromatin-loading-and-its-phosphorylation-in-mantle-cell-lymphoma-cells
#15
Yuling Nie, Tao Lang
AIM: The ATR-interacting protein (ATRIP) is responsible for the recognition of DNA damage-induced structure and regulation of cellular responses to DNA damage and replication stress. The purpose of our study was to identify the underlying mechanism with respect to chromatin loading and phosphorylation of ATRIP in mantle cell lymphoma (MCL). METHODS: JeKo cells were used in our study. Differently tagged ATRIP (Myc-, hemaglutinin (HA) or Flag) and minichromosome maintenance (MCM) complex (MCM2, MCM3, MCM5, and MCM6) were transfected into 293T cells...
November 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29441717/s100a9-gene-silencing-inhibits-the-release-of-pro-inflammatory-cytokines-by-blocking-the-il-17-signalling-pathway-in-mice-with-acute-pancreatitis
#16
Dong-Mei Wu, Shan Wang, Min Shen, Yong-Jian Wang, Bo Zhang, Zi-Qi Wu, Jun Lu, Yuan-Lin Zheng
The study aimed to investigate whether S100A9 gene silencing mediating the IL-17 pathway affected the release of pro-inflammatory cytokines in acute pancreatitis (AP). Kunming mice were assigned to the normal, AP, AP + negative control (NC), AP + shRNA, AP + IgG and AP + anti IL-17 groups. ELISA was applied to measure expressions of AMY, LDH, CRP, TNF-α, IL-6 and IL-8. The cells were distributed into the control, blank, NC, shRNA1 and shRNA2 groups. MTT assay, flow cytometry, RT-qPCR and Western blotting were used to evaluate cell proliferation, cell cycle and apoptosis, and expressions of S100A9, TLR4, RAGE, IL-17, HMGB1 and S100A12 in tissues and cells...
February 14, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29441570/inhibition-of-nox4-dependent-ros-signaling-attenuates-prostate-fibroblast-activation-and-abrogates-stromal-mediated-pro-tumorigenic-interactions
#17
Natalie Sampson, Elena Brunner, Anja Weber, Martin Puhr, Georg Schäfer, Cedric Szyndralewiez, Helmut Klocker
Carcinoma-associated fibroblasts (CAFs) play a key onco-supportive role during prostate cancer (PCa) development and progression. We previously reported that the reactive oxygen species (ROS)-producing enzyme NADPH oxidase 4 (Nox4) is essential for TGFβ1-mediated activation of primary prostate human fibroblasts to a CAF-like phenotype. This study aimed to further investigate the functional relevance of prostatic Nox4 and determine whether pharmacological inhibition of stromal Nox4 abrogates paracrine-mediated PCa-relevant processes...
February 14, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29437010/neuronal-expression-of-junctional-adhesion-molecule-c-is-essential-for-retinal-thickness-and-photoreceptor-survival
#18
Y Li, F Zhang, W Lu, X Li
BACKGROUND: Photoreceptor cell death is a key pathology of retinal degeneration diseases. To date, the molecular mechanisms for this pathological process remain largely unclear. Junctional adhesion molecule-c (Jam-c) has been shown to play important roles in different biological events. However, its effect on retinal neuronal cells is unknown. OBJECTIVE: To determine the effect of Jam-c on adult mouse eyes, particularly, on retinal structure, vasculature and photoreceptor cells, in order to explore potential important target molecules for ocular diseases...
February 12, 2018: Current Molecular Medicine
https://www.readbyqxmd.com/read/29436665/microrna-375-inhibits-glioma-cell-proliferation-and-migration-by-downregulating-rwdd3-in%C3%A2-vitro
#19
Chen-Xing Ji, Yang-Hua Fan, Fan Xu, Shi-Gang Lv, Min-Hua Ye, Miao-Jing Wu, Xin-Gen Zhu, Lei Wu
Derived from brain glial cells, gliomas are currently the most common primary tumours in the central nervous system and are characterised by a high recurrence rate and poor prognosis. RWDD3 (RWD domain-containing sumoylation enhancer, also termed RSUME), which can be induced by cellular stress, such as CoCl2, heat shock and hypoxia, may play a crucial role in tumour angiogenesis, growth and metastasis. MicroRNAs (miRNAs) have been demonstrated to act as negative regulators of post-transcriptional gene expression and are involved in tumour growth and metastasis...
February 13, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29436591/downregulation-of-secreted-clusterin-potentiates-the-lethality-of-sorafenib-in-hepatocellular-carcinoma-in-association-with-the-inhibition-of-erk1-2-signals
#20
Jingtao Zhong, Xiaoming Yu, Xiaofeng Dong, Hong Lu, Wuyuan Zhou, Lei Li, Zhongchao Li, Pengfei Sun, Xuetao Shi
Secretory clusterin (sCLU) is overexpressed in cancer and is associated with resistance to chemotherapy in several types of cancer, including hepatocellular carcinoma (HCC). Sorafenib (SOR), a multikinase inhibitor of Raf/mitogen‑activated protein kinase kinase/extracellular signal‑regulated kinase (ERK) signaling and the receptor tyrosine kinase, is recognized as the standard therapeutic strategy for patients with advanced HCC. However, the role of sCLU in the resistance of HCC to SOR remains to be fully elucidated...
February 6, 2018: International Journal of Molecular Medicine
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