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https://www.readbyqxmd.com/read/30337453/nrt1-1-related-nh4-toxicity-is-associated-with-balance-between-nh4-uptake-and-assimilation
#1
Shao-Fen Jian, Qiong Liao, Hai-Xing Song, Qiang Liu, Joe Eugene Lepo, Chunyun Guan, Jianhua Zhang, Abdelbagi M Ismail, Zhen-Hua Zhang
High concentration of ammonium (NH4+) as sole source of nitrogen in growth media is often toxic to plants. The nitrate transporter NRT1.1 is involved in mediating plant NH4+ toxicity; however, this mechanism remains undefined. In this study, the wild-type Arabidopsis (Col-0) and NRT1.1 mutants (chl1-1 and chl1-5) were grown hydroponically in NH4NO3 and (NH4)2SO4 media to assess the function of NRT1.1 in NH4+ stress responses. All the plants grew normally in mixed N sources, but Col-0 displayed more chlorosis, and lower biomass and photosynthesis than the NRT1...
October 18, 2018: Plant Physiology
https://www.readbyqxmd.com/read/30335828/identification-of-an-apiosyltransferase-in-the-plant-pathogen-xanthomonas-pisi
#2
James Amor Smith, Maor Bar-Peled
The rare branched-chain sugar apiose, once thought to only be present in the plant kingdom, was found in two bacterial species: Geminicoccus roseus and Xanthomonas pisi. Glycans with apiose residues were detected in aqueous methanol-soluble fractions as well as in the insoluble pellet fraction of X. pisi. Genes encoding bacterial uridine diphosphate apiose (UDP-apiose) synthases (bUASs) were characterized in these bacterial species, but the enzyme(s) involved in the incorporation of the apiose into glycans remained unknown...
2018: PloS One
https://www.readbyqxmd.com/read/30333662/structural-characterization-and-raw264-7-murine-macrophage-stimulating-activity-of-a-fucogalactoglucan-from-colpomenia-peregrina
#3
Zeynab Rostami, Mehdi Tabarsa, SangGuan You, Masoud Rezaei
Water-soluble polysaccharides were isolated from Colpomenia peregrina to determine their chemical characteristics and immunomodulatory properties. High extraction yields were obtained for CP1 (17.6%) and CP2 (5.2%) polysaccharides. Polysaccharides were mainly consisted of neutral sugars (67.01-73.79%), uronic acids (9.43-14.89%), proteins (3.44-14.89%) and small amounts of sulfates (4.87-4.91%). Polysaccharides were composed of fucose (20.62-24.56%), galactose (25.5-26.94%) and glucose (50.00-52.91%) residues...
November 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/30333638/evaluation-of-1-methylcyclopropene-1-mcp-treatment-combined-with-nano-packaging-on-quality-of-pleurotus-eryngii
#4
Fangxu Xu, Yefei Liu, Xiufeng Shan, Shenghou Wang
The aim of the present study was to investigate the effect of 1-methylcyclopropene (1-MCP) combined with nano-packaging on quality of pleurotus eryngii stored at 4 ± 1 °C for 12 days. Texture, respiration rate, soluble protein, soluble sugar, weight loss, soluble solid, malondialdehyde (MDA) content, polyphenol oxidase (PPO), superoxide dismutase (SOD) and catalase (CAT) activity of treated pleurotus eryngii were determined. The results suggested that 1-MCP treatment combined with nano-packaging reduce respiration rate, weight loss and MDA content, delayed the decrease of soluble protein content, and maintained soluble sugar and soluble solid content of treated pleurotus eryngii during the storage at 4 ± 1 °C compared with the untreated samples...
November 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/30332822/simultaneous-separation-and-purification-of-five-polymethoxylated-flavones-from-dahongpao-tangerine-citrus-tangerina-tanaka-using-macroporous-adsorptive-resins-combined-with-prep-hplc
#5
Zhenqing Li, Ziyan Zhao, Zhiqin Zhou
In this study, a preparative separation method was established to simultaneously isolate the polymethoxylated flavones (PMFs) from the peel of "Dahongpao" tangerine using macroporous adsorptive resins (MARs) combined with prep-HPLC. The total PMFs were enriched using MARs to remove most sugars, water-soluble pigments, and flavanones, and the eluents obtained were analyzed by ultra-performance liquid chromatography (UPLC) to determine the PMF composition. The separation and purification of PMFs were carried out by using a mass spectrometry-guided prep-HPLC with a gradient elution of acetonitrile-water ( v / v ), simultaneously...
October 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30326850/distinctive-physiological-and-molecular-responses-to-cold-stress-among-cold-tolerant-and-cold-sensitive-pinus-halepensis-seed-sources
#6
Khaled Taïbi, Antonio D Del Campo, Alberto Vilagrosa, José María Bellés, M P López-Gresa, José M López-Nicolás, José M Mulet
BACKGROUND: Forest species ranges are confined by environmental limitations such as cold stress. The natural range shifts of pine forests due to climate change and proactive-assisted population migration may each be constrained by the ability of pine species to tolerate low temperatures, especially in northern latitudes or in high altitudes. The aim of this study is to characterize the response of cold-tolerant versus cold-sensitive Pinus halepensis (P. halepensis) seedlings at the physiological and the molecular level under controlled cold conditions to identify distinctive features which allow us to explain the phenotypic difference...
October 16, 2018: BMC Plant Biology
https://www.readbyqxmd.com/read/30325140/comprehensive-evaluation-and-construction-of-drought-resistance-index-system-in-hydrangea-macrophylla
#7
Jian Guo Cai, Yi Zhang, Ou Wen Sun, Qian Qian Yang
With the continuous drought stress treatment to five Hydrangea macrophylla varieties of different abilities of drought resistance, twenty-five physiological-biochemical indices were mea-sured. We evaluated the drought resistance of different varieties and established the mathematic model. The results showed that leaf mass per area, cell membrane permeability (CMP), the content of malonaldehyde (MDA), the activity of superoxide dismutase (SOD), soluble sugar content, proline content, intercellular carbon dioxide and non-photochemical quenching coefficient were significantly increased under drought stress for twenty days...
October 2018: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
https://www.readbyqxmd.com/read/30319929/solvent-free-method-for-the-copolymerization-of-labile-sugar-derived-building-blocks-into-polyamides
#8
Aleksandra A Wróblewska, Sander Stevens, Wessel Garsten, Stefaan M A De Wildeman, Katrien V Bernaerts
This research focuses on the preparation of biobased copolyamides containing biacetalized galactaric acid (GalX), namely, 2,3:4,5-di- O -isopropylidene-galactaric acid (GalXMe) and 2,3:4,5-di- O -methylene-galactaric acid (GalXH), in bulk by melt polycondensation of salt monomers. In order to allow the incorporation of temperature-sensitive sugar-derived building blocks into copolyamides at temperatures below the degradation temperature of the monomers and below their melting temperatures, a clever selection of salt monomers is required, such that the sugar-derived salt monomer dissolves in the other salt monomers...
October 1, 2018: ACS Sustainable Chemistry & Engineering
https://www.readbyqxmd.com/read/30315881/physicochemical-characterizations-of-polysaccharides-from-angelica-sinensis-radix-under-different-drying-methods-for-various-applications
#9
Ying Wang, Xia Li, Ping Zhao, Zhuo Qu, Detao Bai, Xiaoxiao Gao, Chengcheng Zhao, Jie Chen, Wenyuan Gao
Polysaccharides (ASP) were extracted from Angelica Sinensis Radix (ASR) and were subjected to freeze drying (ASP-FD), vacuum drying (ASP-VD), and hot air drying (ASP-HD). Structural characteristics, rheological and emulsifying properties, antioxidant, anticoagulant and alkaline phosphatase inhibitory activities of ASPs were firstly investigated. ASP-FD owned higher thermostability, unique morphological structure, uniform and the lowest molecular weight, which is suitable for using as a raw material for polysaccharide derivatives...
October 10, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/30315574/yield-and-kernel-nutritional-quality-in-normal-maize-and-quality-protein-maize-cultivars-exposed-to-ozone
#10
Aditya Abha Singh, S B Agrawal, J P Shahi, Madhoolika Agrawal
BACKGROUND: Tropospheric ozone (O3 ) being phytotoxic impacts global food security. Present study investigated yield responses and kernel quality traits of two maize cultivars [DHM117: normal maize (NM)] and [HQPM1: quality protein maize (QPM)]. Cultivars were exposed to two doses of elevated O3 viz. NFC+15 and NFC+30 ppb O3 above ambient level (NFC, non-filtered chambers) while filtered chambers served as control. RESULTS: Test weight (thousand kernel weight), weight of kernels m-2 and kernel starch content reduced more in NM than QPM due to elevated O3 exposure...
October 12, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/30312954/overexpression-of-brassica-campestris-bcice1-gene-increases-abiotic-stress-tolerance-in-tobacco
#11
Tengguo Zhang, Jiangnan Mo, Ke Zhou, Yan Chang, Zigang Liu
In this study, a cDNA of ICE1 (inducer of CBF expression 1) gene, named BcICE1, was isolated from Brassica campestris 'Longyou 6'. The deduced protein has 499 amino acids containing a typical bHLH domain and is highly identical with AtICE1 (85.9%) from Arabidopsis thaliana. BcICE1 is located in the nucleus. The activities of SOD, CAT, POD, and APX and the transcriptional levels of SOD, CAT, and POD genes were higher in BcICE1-transgenic tobacco than in wild-type (WT) tobacco under cold stress. Compared with WT tobacco, proline, soluble sugar, and chlorophyll were enhanced, whereas malondialdehyde and relative conductivity were decreased in BcICE1-transgenic tobacco...
October 1, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/30309609/metabolite-characterization-of-powdered-fruits-and-leaves-from-adansonia-digitata-l-baobab-a-multi-methodological-approach
#12
Arold Jorel Tsetegho Sokeng, Anatoly P Sobolev, Arianna Di Lorenzo, Jianbo Xiao, Luisa Mannina, Donatella Capitani, Maria Daglia
The metabolite profiling of extracts from Adansonia digitata L. (baobab) fruit pulp and leaf, and the quantification of their major components, was conducted by means of reverse-phase, high-performance liquid chromatography with photodiode array detection, coupled to electrospray ion-trap mass spectrometry (RP-HPLC-PDA-ESI-MS/MS) and high field nuclear magnetic resonance (NMR) spectroscopy. Water-soluble metabolites from chemical classes including sugars, amino acids, organic acids, and phenolic compounds, were identified, in addition to metabolites soluble in organic solvents such as triacylglycerides, sterols, and fatty acids, and most of these were quantified...
January 30, 2019: Food Chemistry
https://www.readbyqxmd.com/read/30309578/glycine-betaine-reduces-chilling-injury-in-peach-fruit-by-enhancing-phenolic-and-sugar-metabolisms
#13
Li Wang, Timin Shan, Bing Xie, Chen Ling, Shuang Shao, Peng Jin, Yonghua Zheng
Glycine betaine (GB) treatment is useful to reduce chilling injury (CI) of several kinds of fruits including peach. However, the regulatory mechanism remains unknown. In this study, peach fruit was treated with 10 mmol L-1 GB solution for 10 min. The effects of GB treatment on CI, phenolic and soluble sugar metabolism were investigated in this study. Moreover, phenylpropanoid and soluble sugar content, and enzyme activities associated with phenolic and sugar metabolisms were also measured. The results showed that GB reduced CI and maintained high levels of total phenolic and flavonoid content...
January 30, 2019: Food Chemistry
https://www.readbyqxmd.com/read/30309034/characterization-and-prebiotic-potential-of-longan-juice-obtained-by-enzymatic-conversion-of-constituent-sucrose-into-fructo-oligosaccharides
#14
Yongxia Cheng, Haibo Lan, Lei Zhao, Kai Wang, Zhuoyan Hu
The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide was investigated. The physicochemical properties and carbohydrate composition of the longan juice was evaluated before and after enzymatic treatment. The stimulation effects of the treated longan juice on probiotic bacteria growth were also studied in vitro. The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment...
October 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30305991/anatomical-and-biochemical-analyses-reveal-the-mechanism-of-double-color-formation-in-paeonia-suffruticosa-shima-nishiki
#15
Xinpeng Zhang, Mingyuan Zhao, Jing Guo, Lanyong Zhao, Zongda Xu
Paeonia suffruticosa 'Shima Nishiki' is a very precious double-color cultivar because of its distinctive and colorful flowers. However, our understanding of the underlying mechanisms of its double-color formation is limited. The present study investigated the soluble sugar content, cell sap pH value and anatomical structure, anthocyanin composition and content and expression patterns of genes related to anthocyanin biosynthesis in the red and pink petals of the 'Shima Nishiki' cultivar. Here, we found that soluble sugar content, cell sap pH and the shape of outer epidermal cells were not the key factors that determine double-color formation...
October 2018: 3 Biotech
https://www.readbyqxmd.com/read/30305221/hypoglycemic-bioactivity-of-novel-eco-friendly-carbon-dots-derived-from-traditional-chinese-medicine
#16
Ziwei Sun, Fang Lu, Jinjun Cheng, Meiling Zhang, Yafan Zhu, Yaxue Zhang, Hui Kong, Huihua Qu, Yan Zhao
Although recent research on intrinsic bioactivities of carbon dots is expanding, it is focused on basic aspects, which creates the need for broadening our knowledge about carbon dots to expand their uses in biomedical sciences and facilitate their application in clinical practice. Jiaosanxian is a charcoal traditional Chinese medicine with a pronounced effect on promoting appetite and digestion that is widely used as dyspepsia therapy in China. To study the underlying mechanism and the material requirements, we investigated carbon dots which was generated in the charcoal processing...
December 1, 2018: Journal of Biomedical Nanotechnology
https://www.readbyqxmd.com/read/30304226/light-environment-influences-the-flood-tolerance-in-cordia-americana-l-gottschling-j-s-mill
#17
Caroline G Samojeden, Ághata C Artusi, Heliur A A Delevatti, Silvia V Milesi, Rogério L Cansian, Camila Kissmann, Tanise L Sausen
The subtropical riverine forests present a variation in soil water availability throughout the year, following precipitation seasonality. The objective of this work was to evaluate the responses of Cordia americana to different light intensities combined with soil flooding. Seedlings were acclimated to light treatments, with full sun and shade conditions. Sun and shade plants were subjected to soil flooding during periods of 10 (short) and 30 (longer) days. After 10 days, flooded plants had a higher root dry mass accumulation and soluble sugars content, regardless of the light condition...
July 2018: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/30297684/supplementary-light-source-affects-the-growth-and-development-of-codonopsis-lanceolata-seedlings
#18
Xiuxia Ren, Ya Liu, Hai Kyoung Jeong, Byoung Ryong Jeong
Codonopsis lanceolata is widely used in traditional medicine and diets. However, there is no optimal protocol for the commercial production of C. lanceolata seedlings. This study was carried out to find the optimum supplementary light source for the production of C. lanceolata seedlings. Seedlings were grown for four weeks in a glasshouse with an average daily light intensity of 490 μmol·m-2 ·s-1 photosynthetic photon flux density (PPFD) coming from the sun and a 16-h daily supplementary lighting at 120 μmol·m-2 ·s-1 PPFD from either high-pressure sodium (HPS), metal halide (MH), far-red (FR), white LED (LED-w), or mixed (white: red: blue = 1:2:1) LEDs (LED-mix)...
October 8, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30292109/antioxidant-systems-responses-and-the-compatible-solutes-as-contributing-factors-to-lead-accumulation-and-tolerance-in-lathyrus-sativus-inoculated-by-plant-growth-promoting-rhizobacteria
#19
Souhir Abdelkrim, Salwa Harzalli Jebara, Moez Jebara
Short-term lead (Pb) uptake by plants is important to better understand the mechanisms of metal uptake, plant tolerance and detoxification strategy. Thus we examined the response of Lathyrus sativus to 1 mM Pb application in hydroponic sorption kinetics at 24, 48 and 72 h, and we investigated the contribution of two inocula I1 (R. leguminosarum (M5) + B. simplex + Luteibacter sp + Variovorax sp) and I5 (R. leguminosarum (M5) + P. fluorescens (K23) + Luteibacter sp + Variovorax sp) in plant mechanisms responses...
December 30, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/30290333/possible-interaction-of-ros-antioxidants-and-aba-to-survive-osmotic-stress-upon-acclimation-in-vigna-radiata-l-wilczek-seedlings
#20
Moumita Sahu, Rup Kumar Kar
Acclimation is a process of adjustment to gradual environmental change that enables plants to survive further stress by triggering some tolerance mechanism possibly involving ABA, ROS and oxidative metabolism. Here we have studied acclimation responses in terms of the performances with regard to physiological (growth and relative water content) and biochemical (chlorophyll, carotenoids, protein, malondialdehyde, sugar content) attributes, ABA production and stomatal sensitivity to exogenous ABA, extracellular ROS production and activation of antioxidant system...
September 28, 2018: Plant Physiology and Biochemistry: PPB
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