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Seminal plasma

Tamás Müller, Tamás Szabó, Tímea Kollár, Balázs Csorbai, Zoran Marinović, László Horváth, Balázs Kucska, Ádám Bodnár, Béla Urbányi, Ákos Horváth
In this study, we aimed to develop a practical protocol for using cryopreserved sperm for induced/wild/tank spawning of fish species with external fertilization. Experiments were carried out on African catfish (Clarias gariepinus) as a model species. Sperm was collected for cryopreservation and diluted with the cryomedium (266 mM fructose, 20% methanol) at a ratio of 1:1 with a final methanol concentration of 2.47 M pH7.73. Diluted sperm was loaded into 0.5-ml straws and cryopreserved by conventional protocol...
September 27, 2018: Theriogenology
M Slowinska, A Hejmej, J Bukowska, E Liszewska, B Bilinska, P Hliwa, K Kozlowski, J Jankowski, A Ciereszko
Yellow semen syndrome (YSS) is the most widely recognized problem among male turkeys. Yellow semen is of low quality and, when used for insemination, results in reduction of fertility and hatchability. Elevated level of serum albumin-like protein accession no. XP_003205725 is a characteristic feature of yellow seminal plasma suggesting albumin role in YSS pathology. However, knowledge regarding the expression of albumin in the reproductive tract in relation to YSS is very limited. The aim of this study was to identify albumin secretion and localization sites in the turkey reproductive tract in relation to YSS...
October 10, 2018: Poultry Science
Mahsa Darbandi, Sara Darbandi, Ashok Agarwal, Saradha Baskaran, Pallav Sengupta, Sulagna Dutta, Pooneh Mokarram, Kioomars Saliminejad, Mohammad Reza Sadeghi
Kelch-like ECH-associated protein 1 (keap1)-nuclear factor-erythroid 2-related factor 2 (Nrf2) pathway is one of the master regulators of cellular defence against oxidative stress. Epigenetic alterations like hypermethylation of keap1 gene impair keap1-Nrf2 system in several oxidative stress-associated diseases. The objective of this study was to evaluate the epigenetic status of keap1 in sperm DNA of normozoospermic subjects, having different levels of reactive oxygen species (ROS) in seminal plasma. Semen samples were obtained from 151 apparently healthy male partners of couples who attended the Avicenna infertility clinic...
October 8, 2018: Andrologia
Zhen-Zhen Wan, Heng-Gui Chen, Wen-Qing Lu, Yi-Xin Wang, An Pan
BACKGROUND: Exposure to high levels of metals/metalloids may impair semen quality. Computer-aided sperm analysis (CASA) can be used for kinematic analysis of spermatozoa, which provides additional insights into sperm motion characteristics. OBJECTIVE: To explore the associations of urinary and seminal plasma metal/metalloid concentrations with CASA motion parameters and assess the degree of correspondence between the two sample types. METHODS: Eighteen metals/metalloids in seminal plasma and repeated urine samples were determined among 746 men recruited from a reproductive center...
October 2, 2018: Chemosphere
Thanapol Nongbua, Essraa M Al-Essawe, Anders Edman, Anders Johannisson, Jane M Morrell
SummaryThe aim of this study was to investigate the effect of adding homologous or heterologous bovine seminal plasma (SP) to SP-free sperm samples before freezing on sperm quality after thawing. Ejaculates from bulls of known fertility were used as a source of SP. The SP was removed from further aliquots of the same ejaculates by colloid centrifugation to create SP-free sperm samples; the resuspended sperm pellets were treated with homologous or heterologous SP from high or low fertility bulls at 0%, 1% or 5% before freezing...
October 5, 2018: Zygote: the Biology of Gametes and Early Embryos
Zahra Kanannejad, Behrouz Gharesi-Fard
Unexplained male infertility (UMI) is a condition in which routine semen analysis fails to detect subcellular sperm dysfunctions. In the present research, a comparative proteomics study of seminal plasma (SP) was conducted in men with unexplained infertility whose female partners had undergone in vitro fertilisation (IVF) treatment to find differences in the SP protein profile. Five UMI men with successful and eight with unsuccessful IVF outcome enrolled in this study. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique was used for protein separation...
October 1, 2018: Andrologia
Mohamed Badawy Abdel-Naser, Andreas Altenburg, Christos C Zouboulis, Uwe Wollina
Schistosomiasis (bilharziasis) is an endemic parasitic disease of a major source of morbidity and mortality for developing countries. It is caused by different species of blood flukes (trematode worms) of the genus Schistosoma, the most important of which are S. haematobium which infests the genitourinary tract and S. mansoni and S. japonicum which infest the gastrointestinal tract. The incidence of schistosome-induced male infertility is not known and probably underestimated being overshadowed by the more severe and often fatal urinary and gastrointestinal complications...
October 1, 2018: Andrologia
K A El-Bahrawy
Assisted reproductive technologies have been reported to improve reproductive efficiency and genetic potential in camelids. Two experiments were carried out to determine efficiency of centrifugation in the presence of a mucolytic agent for liquefaction of dromedary semen. In the first experiment, three groups, namely: I. Tris lactose (TL, control), II. Tris lactose supplemented with amylase (TL_A) and III. Tris lactose supplemented with amylase followed by seminal plasma removal via centrifugation (TL_A_Cent...
December 2017: International Journal of Veterinary Science and Medicine
Michał Dobrakowski, Zbigniew Kaletka, Anna Machoń-Grecka, Sławomir Kasperczyk, Stanisław Horak, Ewa Birkner, Jolanta Zalejska-Fiolka, Aleksandra Kasperczyk
The aim of the study was to investigate the associations between standard semen parameters and the parameters reflecting oxidative stress intensity, antioxidant defense functions, levels of selected macro and trace elements, and parameters characterizing immune system function. The study group consisted of 103 fertile males. Based on semen volume, sperm concentration, total sperm count, and percentage of motile sperm at 1 hour postcollection, the individuals were divided into two equal groups-those with excellent (EX) semen quality and those with mediocre (ME) semen quality...
2018: Oxidative Medicine and Cellular Longevity
Kemal Gumus, Mehmet Gulum, Ercan Yeni, Halil Ciftci, Yigit Akin, Emre Huri, Hakim Celik, Ozcan Erel
PURPOSE: In limited number of studies performed concerning the psychological moods of female, and male with the diagnosis of infertility, data related to increased incidence of depression, and anxiety have been reported. The objective of this study is to determine whether azoospermia has any psychological effects on men, and investigate the potential effects of psychological mood on seminal, and plasma oxidative parametres. MATERIALS AND METHODS: Twenty-seven patients whose two consecutive semen analyses were reported as pellet -negative azoospermia constituted the azoospermic group, and 30 healthy individuals who applied to the infertility polyclinic with normal seminal parametres comprised the normozoospermic group...
September 22, 2018: Urology Journal
Zahid Naseer, Ejaz Ahmad, Hande Sultan Şahiner, Erkmen Tuğrul Epikmen, Muhammad Fiaz, Muhamad Rizwan Yousuf, Shahzad Akbar Khan, İlker Serin, Ahmet Ceylan, Melih Aksoy
This study focused to determine beneficial impact of feeding quercetin supplemented diet on semen quality in summer heat imposed rabbits. Twelve heat stressed (HS) adult rabbits bucks were either fed with basal diet (HS; n = 06) or quercetin supplemented diet (QU-HS; n = 06) for a period of 56 days. Semen samples were collected and evaluated for volume, osmolality, morphology, concentration, motility, motion kinetics, viability, acrosome integrity, mitochondrial potential, and seminal plasma MDA level...
September 15, 2018: Theriogenology
M H Abaspour Aporvari, M Mamoei, S Tabatabaei Vakili, M Zareei, Navid Dadashpour Davachi
Zinc is an essential mineral and accepted as a trace element in the animal nutrition and for its role in biological enzymatic pathways. This study aimed to investigate the effect of oral administration of zinc oxide nanoparticles (ZnONPs) on some of the antioxidant parameters of semen plasma, quantitative and qualitative properties of Arabic ram sperm in the non-breeding season. Twelve adult Arabic rams (about 3-5 years old, 70 ±2.1 kg) were randomly assigned to receive one of the three levels of dietary ZnONPs (control; 0, group 1; 40 ppm and group 2; 80 ppm)...
June 2018: Archives of Razi Institute
Giacomo Lazzarino, Ilaria Listorti, Luigi Muzii, Angela Maria Amorini, Salvatore Longo, Enrico Di Stasio, Giuseppe Caruso, Serafina D'Urso, Ilaria Puglia, Giuseppe Pisani, Giuseppe Lazzarino, Barbara Tavazzi, Pasquale Bilotta
STUDY QUESTION: Is the determination of antioxidants, oxidative/nitrosative stress-related compounds, purines, pyrimidines and energy-related metabolites in human seminal plasma of utility to evidence biomarkers related to male infertility? SUMMARY ANSWER: The determination of 26 metabolites in seminal plasma allowed to evidence that 21/26 of them are biomarkers of male infertility, as well as to calculate a cumulative index, named Biomarker Score, that fully discriminates fertile controls from infertile patients and partially differentiates infertile without from infertile with spermiogram anomalies...
October 1, 2018: Human Reproduction
Mathias Abiodun Emokpae, Happy Ngozi Chima
Background: Male senescence may affect testicular function, sperm indices and generation of high levels of oxidants and apoptosis. Objective: This study evaluates the effect of male age on the expression of some apoptosis and oxidative stress markers in seminal fluid of males investigated for infertility in a tertiary health institution in Nigeria. Materials and Methods: In this cross-sectional study, 122 men aged 20-60 yr who were investigated for infertility and were stratified according to age into four groups...
July 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
Nittaya Phanuphak, Supanit Pattanachaiwit, Tippawan Pankam, Nipat Teeratakulpisarn, Parinya Chamnan, Panita Pathipvanich, Suchart Thongpaen, Siriporn Nonenoy, Jureeporn Jantarapakde, Supabhorn Pengnonyang, Deondara Trachunthong, Thanthip Sungsing, Kittiyaporn Parasate, Sriprai Seeneewong Na Ayutthaya, Ketmookda Trairat, Kanitta Pussadee, Cheewanan Lertpiriyasuwat, Praphan Phanuphak
INTRODUCTION: Sexually transmitted infections (STIs) are common among HIV-positive men who have sex with men (MSM). There have been concerns that undiagnosed and untreated STIs could undermine efforts to use antiretroviral therapy (ART) for prevention due to genital secretion infectiousness. We evaluated the correlation between STIs and HIV RNA in anogenital compartments among HIV-positive MSM before and after ART. METHODS: MSM participants newly diagnosed with HIV were offered ART regardless of CD4 count during November 2012 to November 2015...
September 2018: Journal of the International AIDS Society
Anna Domoslawska, Slawomir Zdunczyk, Monika Franczyk, Marta Kankofer, Tomasz Janowski
The aim of this study was to evaluate the total antioxidant capacity and protein peroxidation intensity in seminal plasma of infertile and fertile dogs. The study was conducted on 10 infertile and 10 fertile dogs of various breeds. Infertility was defined as conception failure at least 3 matings with different bitches. Semen was collected by manual manipulation. The sperm concentration and motility parameters were evaluated using CASA Hamilton Thorne, Vers. IVOS 12.3. The morphology of spermatozoa and the percentage of live and dead sperm cells were assessed microscopically, total antioxidant capacity and the content of SH-groups in seminal plasma were determined spectrophotometrically, the contents of protein peroxidation markers in seminal plasma, bityrosine and formylokinurenine, were determined using spectrofluorimetric methods...
September 17, 2018: Reproduction in Domestic Animals, Zuchthygiene
D F Leal, M A Torres, G M Ravagnani, S M M K Martins, F V Meirelles, A F C de Andrade
Seminal plasma (SP), the fluid that surrounds the sperm cells, is known to exert substantial influence on sperm physiology. The SP has a pivotal role in sperm function in vivo, and due to its components, it functions in an ambiguous manner in vitro, simultaneously possessing deleterious and beneficial effects. This experiment aimed to describe the differences between the presence or absence of SP from the sperm-rich fraction on some spermatozoa characteristics (kinetics, plasma and acrosome membrane integrity, lipid peroxidation and capacitation-like changes)...
August 26, 2018: Animal Reproduction Science
Jamie L Stewart, Igor F Canisso, Robyn E Ellerbrock, Vitor R G Mercadante, Fabio S Lima
Nerve Growth Factor-β (NGF) is a seminal plasma protein that regulates sperm physiology in bulls, yet its production and association with fertility remain unclear. The objective of this study was to describe NGF distribution in bull accessory sex glands, quantify its seminal plasma concentrations, and determine its association with sire conception rates. Accessory sex glands were collected post-mortem from mature bulls (n = 3). Quantitative PCR and immunohistochemistry were performed in tissue samples. Pre-ejaculate and sperm-rich fraction of seminal plasma from Angus (n = 42) and sperm-rich fraction from Holstein (n = 10) bulls were collected by electroejaculation...
October 2018: Animal Reproduction Science
Kayla A Ross, David S Kolb, Alysson Macedo, Marion Anderson, Claudia Klein
This study tested the hypothesis that the presence of prostaglandin E2 in seminal plasma would aid in the transport of phenolsulfonphthalein (PSP) across the uterotubal junction. Five mares in estrus were inseminated during estrus with PSP dissolved in phosphate-buffered saline and during the subsequent estrus with PSP added to a standard insemination dose. Serum and urine samples were obtained at hours 0, 1, 2, and 3 following treatment and examined for the presence of PSP. Phenolsulfonphthalein could not be detected in any of the urine samples collected from mares following either treatment...
September 2018: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
Momin Momin, Devrim Memiş
Sperm quality parameters in rainbow trout (Oncorhynchus mykiss) were investigated during normal season spawning (November-January) and out-season spawning (July-August) treated with artificial photoperiod manipulation. Normal spawning males (n = 15) were kept in an open concrete pond under natural condition. Out-season spawning males (n = 15) were treated with artificial LED light (50 lm/m2 ) in a closed concrete pond. In these two experimental groups, five fish were used in each of three spawning periods...
September 7, 2018: Fish Physiology and Biochemistry
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