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Seminal plasma

Ghada Ali Omran, Hisham Diab Gaber, Nashwa Ahmed Mohamad Mostafa, Reham Maher Abdel-Gaber, Emadeldeen Ali Salah
Bisphenol A (BPA) is an endocrine disruptor that is widely produced as a by-product in the plastics industry. The aim of the current study was to identify any association between urinary BPA levels and infertility related factors in patients who presented to the andrology clinic of a university hospital in Upper Egypt. In this case-control study, urinary BPA levels in 50 infertile patients and 50 matched controls were measured by high-performance liquid chromatography. Semen quality (sperm concentration, morphology, and motility) and oxidative stress (total antioxidant activity and malondialdehyde levels) were determined along with sperm DNA damage via alkaline comet assays...
August 10, 2018: Reproductive Toxicology
G Restrepo, B Rojano, A Usuga
Cysteine-rich secretory protein-3 (CRISP-3) and some of its non-synonymous polymorphism have been related to the fertility and freezability of stallion semen, however, the role of the CRISP-3 gene and its seminal plasma protein in the raw semen quality is still unknown. The aim of this study was to evaluate the relationship of CRISP-3 with semen quality in stallions. DNA was obtained from blood samples of 100 stallions, from which 30 stallions were randomly selected to obtain 60 ejaculates. Through PCR amplification and sequencing, the variation of four non-synonymous SNPs from CRISP-3 was identified and haplotypes were derived...
August 12, 2018: Reproduction in Domestic Animals, Zuchthygiene
Vidya Sagar Reddy, Brijesh Yadav, Chhote Lal Yadav, Mukul Anand, Dilip Kumar Swain, Devendra Kumar, Deepika Kritania, Arun Kumar Madan, Jitender Kumar, Sarvajeet Yadav
Cryopreservation results in substantial deterioration of heat shock protein 70 (HSP70) and ultra-structural changes in sperm organelles, resulting in a marked reduction in post-thaw semen quality. The present study was aimed to explicate the effect of sericin supplementation on expression profile of HSP70, redox status and post-thaw semen quality in Barbari goat. Five Barbari bucks were used to collect thirty semen ejaculates by using artificial vagina and each ejaculate was divided into three aliquots to which sericin was supplemented at 0% (Control), 0...
August 9, 2018: Cryobiology
Burak Evren İnanan, Fevzi Yılmaz
All-male or all-female progeny is preferable in reproduction of fish species. In rainbow trout, all-female progeny is desirable, and the most common and healthful approach is to use sex reversed (SR) females as broodstock by including androgen hormones through the diet at the larval stage. The purpose of this study was to determine the differences and changes of seminal plasma ions (Na+ , K+ , Ca2+ , Mg2+ and Cl- ) and pH in 2 to 3 and 3 to 4 year-old SR females, compared with 2 to 3 year-old normal (N) males at the beginning, middle, and end of the spawning season (SS)...
July 29, 2018: Animal Reproduction Science
Magdi M Waheed, Ahmad M A Meligy, Suror A Dhalam
A total of 14 camels of known fertility (controls) at the Camel Research Centre, King Faisal University and 41 infertile dromedaries brought to the Veterinary Teaching Hospital were used in this study during the breeding season. Seminal plasma and serum were obtained from all males, and stored at -80°C until analysis. Concentrations of six trace elements (manganese [Mn], zinc [Zn], iron [Fe], selenium [Se], copper [Cu] and chromium [Cr]) were estimated in the seminal plasma and serum using an atomic absorption spectrophotometer...
August 4, 2018: Reproduction in Domestic Animals, Zuchthygiene
Nan Wang, Shuang Qu, Wu Sun, Ziyi Zeng, Hongwei Liang, Chen-Yu Zhang, Xi Chen, Ke Zen
Modification of nucleotides significantly increases the diversity of functional nucleic acids. As one of the most common modifications of RNAs, methylation of the 2'-hydroxyl-group of ribonucleotides (2'-O-methylation) has been found in various RNAs in eukaryotes. However, due to lack of efficient method for quantifying small RNA 3' terminal 2'-O-methylation, it is difficult to monitor the dynamic change of 3' terminal 2'-O-methylation during various biological process. Capitalizing on the finding that 3' terminal RNA 2'-O-methylation can inhibit the activity of poly(A) polymerase, an enzyme used in real-time quantitative polymerase chain reaction (RT-qPCR) assay, here we develop a poly(A)-tailed RT-qPCR method by which the 2'-O-methylation level of small RNAs such as microRNAs (miRNAs) and Piwi-interacting RNAs (piRNAs) can be directly quantified...
August 3, 2018: RNA
Sandeep Kumar Chaudhary, Asit Baran Mandal, Rasbihari Bhar, M Gopi, A Kannan, Sunil E Jadhav, Jaydip J Rokade
Objective: This study investigated the effects of soapnut (Sapindus mukorossi) shell powder (SSP) on serum hormone level, egg quality, semen characteristics and reproductive performance of broiler breeders fed with the maize-soybean meal based diet. Methods: Ninety six female and twenty four male CARIBRO-VISHAL broiler breeders, 38-week old, were individually caged and randomly allocated to four treatment groups (24 female breeders/treatment and 6 male breeders/treatment): an un-supplemented control (T1) and three groups with 0...
July 26, 2018: Asian-Australasian Journal of Animal Sciences
Ibrahim Rashid, Md Shakhawate Hossain, Mohammad Abdus Salam, S M Rafiquzzaman
Seminal composition and semen quality are the important determinants in assessing the reproductive performance of different fishes. This study was carried out to evaluate the seminal composition and sperm quality of Barbonymus gonionotus. The seminal plasma contained 17.2 ± 0.34 mmol/l, 20.9 ± 0.48 mmol/l, 0.72 ± 0.04 mmol/l, 3.8 ± 0.2 mmol/l, and 1.49 ± 0.02 g/dl of Na+ , K+ , Ca++ , Mg++ , and total protein, respectively. The physical spermatological parameters, such as sperm volume, sperm motility, motility duration, sperm density, osmolality, and pH values were 1...
July 25, 2018: Fish Physiology and Biochemistry
Essraa M Al-Essawe, Anders Johannisson, Manuela Wulf, Christine Aurich, Jane M Morrell
Freezing and thawing processes induce structural and functional damage to sperm plasma membranes and internal organelles. Adding seminal plasma (SP) has been found to minimize or repair the cryoinjuries in some species. The objective of this study was to investigate whether adding SP from stallions of known freezability after thawing could repair cryoinjuries. Semen was collected from warmblood stallions (n = 8, three ejaculates/stallion) and processed by Single Layer Centrifugation (SLC) to remove SP prior to freezing...
June 25, 2018: Animal Reproduction Science
Sophie Palierne, Patricia Meynaud, Alexis Bilmont, Maxence Delverdier, Marie-Odile Semin, Mailys Stieglitz, Guillaume Riviere, André Autefage
The objective of this study was to compare the clinical, biological, macroscopic, and histologic outcomes after resection of the soft palate by plasma-mediated bipolar radiofrequency ablation (PBRA) or traditional incisional techniques (incisional soft palate resection [INC]) in dogs. Ten dogs were divided in two groups. In the INC group, the soft palate was incised with scissors and the wound was sutured in a continuous pattern. In the PBRA group, a wand was used to ablate the desired portion of the soft palate, without suture...
July 24, 2018: Journal of the American Animal Hospital Association
S Mahmood, A Kumar, R Singh, M Sarkar, G Singh, M R Verma, G V P P S R Kumar
Aim: The aim of this study was to evaluate the relationship of scrotal circumference (SC) with plasma testosterone, seminal vesicles (SVs) weight, and its secretion as measurable indicators of fertility and also to sequence and establish phylogenetic relatedness of certain SV protein genes with other species as such integrated approach is lacking. Materials and Methods: Altogether, 59 apparently healthy male buffaloes sacrificed at slaughterhouse were selected (irrespective of breed) for measuring SC and collecting blood and paired SVs...
June 2018: Veterinary World
Kun Li, Rui Li, Ya Ni, Peibei Sun, Ye Liu, Dan Zhang, Hefeng Huang
BACKGROUND: Sperm selection is essential for the health of offspring conceived via assistive reproductive technology (ART). Various methods of sperm preparation for in vitro fertilization and intracytoplasmic sperm injection have been developed to acquire sperm with better quality and to avoid potential genetic disorders. However, current sperm processing and selection techniques bypass the natural selection that occurs during fertilization in vivo. The aim of this study was to present a novel distance-progesterone-combined selection approach with an original device based on the human female reproductive tract, and to report on its effectiveness based on sperm progressive motility, as well as chemotaxis...
July 20, 2018: Journal of Translational Medicine
Yi-Xin Wang, Heng-Gui Chen, Xian-Dong Li, Ying-Jun Chen, Chong Liu, Wei Feng, Qiang Zeng, Peng Wang, An Pan, Wen-Qing Lu
Widespread human exposure to vanadium has been well documented. Vanadium exposure was reported to induce male reproductive toxicity in toxicological studies, yet human epidemiologic studies are lacking. Here we determined the associations between environmental exposure to vanadium and semen quality, spermatozoa DNA damage and serum reproductive hormones. Concentrations of vanadium in seminal plasma and repeated urine samples were determined among 764 men recruited from a reproductive medicine centre. Associations of vanadium concentrations with semen quality parameters (n = 764), DNA integrity measures (n = 404) and serum reproductive hormones (n = 381) were assessed by logistic or linear regression models with adjustment for potential confounders...
July 16, 2018: Science of the Total Environment
Naina Kumar, Amit Kant Singh
Male factor is responsible for 40-50% of infertility cases, contributing equally as a female factor. Oxidative stress has now emerged as one of the major causes of unexplained male infertility, as it leads to increased risk of Deoxyribonucleic acid (DNA) fragmentation, decreased sperm motility, concentration and abnormal morphology. Though a certain amount of reactive oxygen species (ROS) activity is essential for normal functioning of sperms, an excess lead to pathological effects on semen parameters, ultimately resulting in male sub-fertility or infertility...
July 16, 2018: Journal of Gynecology Obstetrics and Human Reproduction
Melissa M Smarr, Kurunthachalam Kannan, Liping Sun, Masato Honda, Wei Wang, Rajendiran Karthikraj, Zhen Chen, Jennifer Weck, Germaine M Buck Louis
BACKGROUND: Some non-persistent endocrine disruptors (EDCs) are adversely associated with semen quality and few studies have measured those EDCs in seminal plasma. OBJECTIVE: To find an association between EDCs in seminal plasma and semen quality parameters. METHODS: Five chemical classes of non-persistent EDCs were quantified in seminal plasma from 339 male partners who participated in a prospective pregnancy study. Bisphenols, benzophenone UV-filters, antimicrobials and phthalate diesters and their monoester metabolites were measured using high performance liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry...
July 4, 2018: Environmental Research
Kambiz Gilany, Naser Jafarzadeh, Ahmad Mani-Varnosfaderani, Arash Minai-Tehrani, Mohammed Reza Sadeghi, Mahsa Darbandi, Sara Darbandi, Mehdi Amini, Babak Arjmand, Zhamak Pahlevanzadeh
Background: Non-obstructive azoospermia (NOA) occurs in approximately 10% of infertile men. Retrieval of the spermatozoa from the testicle of NOA patients is an invasive approach. Seminal plasma is an excellent source for exploring to find the biomarkers for presence of spermatozoa in testicular tissue. The present discovery phase study aimed to use metabolic fingerprinting to detect spermatogenesis from seminal plasma in NOA patients as a non-invasive method. Methods: In this study, 20 men with NOA were identified based on histological analysis who had their first testicular biopsy in 2015 at Avicenna Fertility Center, Tehran, Iran...
April 2018: Journal of Reproduction & Infertility
Ali Fallah, Azadeh Mohammad-Hasani, Abasalt Hosseinzadeh Colagar
Zinc (Zn) is the second most abundant trace element in human, which can't be stored in the body, thus regular dietary intake is required. This review explained the physiological and pathogenesis roles of zinc in men's health and its potentials in germination, quality of sperm, and fertilization. Our investigation showed that Zn contained many unique properties in human, especially males. The antioxidant quality is one of them. Also, the increased reactive oxygen species levels in the seminal plasma of men who are both infertile and smokers influence the Zn content of seminal plasma in a way that physiology of spermatozoa can be affected as well...
April 2018: Journal of Reproduction & Infertility
Yazmín Arellano, Eugene Bratoeff, Yvonne Heuze, Marisol Bravo, Juan Soriano, Marisa Cabeza
It is known that the growth of prostate metastatic bone tumor depends on androgens, and tumor formation can start from migratory malignant cells produced in that organ. These cells exhibit grater type 1 5α-reductase (5α-R1) activity than type 2 5α-reductase. Noteworthy, both isozymes convert testosterone (T) to the more active androgen dihydrotestosterone (DHT) in the target tissues. Thus, in order to potentially improve the prognosis of this disease, in this work, seven derivatives of 17-(1H-benzimidazol-1-yl)-16-formillandrosta-5,16-dien-3β-yl benzoate (4a-f) and 17-(1H-benzimidazol-1-yl)-3-hydroxy-16-formylandrost-5,16-diene (4) were synthesized, characterized and identified as inhibitors of type 1 5α-reductase (5αR1)...
June 23, 2018: Bioorganic & Medicinal Chemistry
Robert Hermes, Thomas Bernd Hildebrandt, Frank Göritz
At times when rhinoceros are fiercely poached, when some rhinoceros species are closer than ever to extinction, and when the scientific community is in debate over the use of advanced cell technologies as a remaining resort it is time to simplify and improve existing assisted reproduction techniques to enhance breeding and genetic diversity in the living populations under our care. Semen cryopreservation has been performed in all captive rhinoceros species with limited degree of success. Here we tested three freezing extenders, containing different cryoprotectants and various freezing rates for the cryopreservation of rhinoceros sperm from 14 bulls...
2018: PloS One
Giuseppe Grande, Domenico Milardi, Silvia Baroni, Giovanni Luca, Alfredo Pontecorvi
Male accessory gland infection/inflammation (MAGI) is a frequent disease, mostly with a chronic course, involving 1 or more sexual accessory glands. Majority of the MAGIs remain asymptomatic, thereby leading to a dilemma whether to treat these patients or not. It is moreover noteworthy that the diagnosis of MAGI is difficult, since patients are frequently asymptomatic and semen samples or prostatic secretions are often free from bacteria. As a consequence the identification of novel and reliable markers of inflammation in seminal plasma is an open challenge...
July 10, 2018: American Journal of Reproductive Immunology: AJRI
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