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Seminal plasma

Woo-Sung Kwon, Ye-Ji Kim, Do-Yeal Ryu, Ki-Jin Kwon, Won-Hee Song, Md Saidur Rahman, Myung-Geol Pang
Fms-like tyrosine kinase 3 (FLT3) is a type III kinase that is highly expressed in seminal plasma of infertile men. FLT3 activation can be blocked by inhibition of its phosphorylation using the nontoxic and selective inhibitor, quizartinib. We investigated the function of FLT3 and the corresponding effects of quizartinib in mouse spermatozoa. Spermatozoa were treated with different concentrations (0.1, 1, 10, 20, and 30 μM) of quizartinib for 90 min at 37 °C in 5% CO2 in air. FLT3 was detected in capacitated and non-capacitated spermatozoa...
December 8, 2018: Theriogenology
Donatella Paoli, Giulia Pecora, Francesco Pallotti, Fabiana Faja, Marianna Pelloni, Andrea Lenzi, Francesco Lombardo
STUDY QUESTION: Is ageing associated with a decline in semen quality and molecular changes to human sperm? SUMMARY ANSWER: Semen quality declines with advancing age and characteristic molecular changes take place during the ageing process, including increased sperm DNA damage, altered sperm protamination and altered seminal plasma miRNA profile. WHAT IS KNOWN ALREADY: During ageing, the reproductive system is exposed to physiological changes and potentially damaging factors that may impair testicular function...
December 14, 2018: Human Reproduction
Wael A Khalil, Mostafa A El-Harairy, Alaa E B Zeidan, Mahmoud A E Hassan
The aim of this study was to evaluate the effect of adding different concentrations of selenium nano-particles (Se-NPs) in semen extender on bull sperm cryopreservation. Five healthy, fertile Friesian bulls were used, and the ejaculates were obtained using an artificial vagina. Semen of all bulls were pooled and diluted in a tris-yolk fructose (TYF) extender supplemented with Se-NPs at concentrations of 0 (T1, control), 0.5 (T2), 1.0 (T3) and 1.5 (T4) μg/ml for a final sperm concentration of 80 × 106 sperm cells/ml...
December 5, 2018: Theriogenology
S X Tang, H L Zhou, Y L Ding, P Yang, Q Chen, X Chen
Objectives: To analyze the factors affecting the patency rate of microsurgical single-stitch longitudinal intussusception vasoepididymostomy and to explore the possible causes of failure. Methods: The clinical data of 87 patients with epididymal obstructive azoospermia who underwent microsurgical vasoepididymostomy from January 2015 to February 2018 in the First Affiliated Hospital of Fujian Medical University were analyzed retrospectively. We observed the patency rate postoperatively and evaluated several factors that possibly related to the patency rates...
December 11, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
R K Kasimanickam, V R Kasimanickam, A Arangasamy, J P Kastelic
Sperm are highly specialized compartmentalized cells, with unique compositional, morphological and functional properties, including a plasma membrane that undergoes dynamic protein remodeling and surface modifications. Seminal plasma is a highly complex biological fluid containing proteins, amino acids, enzymes, fructose and other carbohydrates, lipids, major minerals and trace elements. Seminal plasma proteins are involved in regulation of osmotic pressure and pH of seminal plasma, transport of ions, lipid and hormones...
November 28, 2018: Theriogenology
Iman Al-Saleh, Serdar Coskun, Inaam Al-Doush, Tahreer Al-Rajudi, Reem Al-Rouqi, Mai Abduljabbar, Saad Al-Hassan
This prospective study of 599 couples seeking fertility treatment and who were recruited between 2015 and 2017 was conducted to (a) explore the associations between phthalate exposure and in vitro fertilization (IVF) outcomes; and (b) examine the implication of oxidative stress as a mediator of these. We measured eight phthalate metabolites in two spot urine samples; oxidative stress biomarkers such as malondialdehyde, 8-hydroxy-2-deoxyguanosine, hydrogen peroxide, catalase (CAT), and total antioxidant capacity in follicular fluid and seminal plasma...
November 16, 2018: Environmental Research
Umme K Rima, Abu S M Bari, Mohammad Z Hossain, Mohammad A H Khan
Background: Prostate hyperplasia and neoplasia are major illness of men and elderly dogs. Treatment of prostate cancer requires androgen deprivation surgery or therapy to prevent metastases and alleviate pain. Recently, six DNA vaccines have entered clinical trials against prostate cancer in humans with limited success. There is a need for new therapies that delay the establishment of malignancy and prolong survival. Materials and methods: A plasmid DNA vaccine coding for eight gonadotrophin-releasing hormone (GnRH-I) interspersed in eight T-helper epitopes was used...
December 2018: Prostate International
Anne Grosen, Mona Bungum, Lisbet Ambrosius Christensen, Eugenia Cordelli, Ole Halfdan Larsen, Giorgio Leter, Mette Julsgaard, Thea Vestergaard, Paola Villani, Christian Lodberg Hvas, Jens Kelsen
Background & Aims: The impact of severe inflammation on semen quality, including sperm DNA integrity, in men with inflammatory bowel disease (IBD) is unknown, as are the potential effects of anti-tumour necrosis factor-alpha (TNF-alpha) therapy. We investigated the influence of severe active IBD and anti-TNF-alpha treatment on semen quality. Methods: We prospectively included 20 patients admitted with severe active IBD. Further, 19 patients who initiated and 17 who stopped anti-TNF-alpha therapy were included...
November 30, 2018: Journal of Crohn's & Colitis
Chunrong Lv, Guoquan Wu, Qionghua Hong, Guobo Quan
The cryotolerance of farm animal spermatozoa varies according to their specific features, such as size, shape, and lipid composition. Thus, it is impossible to develop a standardized freezing procedure for different kinds of livestock species. The establishment of an efficient semen cryopreservation procedure will facilitate long-term conservation of small ruminant genetic resources and extension of artificial insemination in daily production. Different from sheep, goat seminal plasma contains a phospholipase, which can affect spermatozoa viability through interaction with milk or egg yolk...
November 30, 2018: Biopreservation and Biobanking
Maria B R Andrade, Ricardo P Bertolla, Paula Intasqui, Mariana P Antoniassi, Danielle S Tibaldi, Larissa B Belardin, Deborah M Spaine
To verify if quality of spermatozoa from men with testicular germ cell tumours is better before or after orchiectomy. This prospective study was carried out including 24 patients with testicular germ cell tumours, who provide one semen sample before they were submitted to unilateral orchiectomy and one other semen sample 30 days after the surgery. After collection by masturbation and liquefaction, an aliquot of the semen sample was used for semen analysis and another was used to evaluate sperm mitochondrial activity, DNA fragmentation and acrosome integrity...
November 29, 2018: Andrologia
J Vitku, L Kolatorova, C Ricco, C Ferroud, O Hennebert, T Skodova, J Heracek, L Starka
7beta-hydroxy-epiandrosterone (7beta-OH-EpiA) is an endogenous androgen metabolite that has been shown to exert neuroprotective, anti-inflammatory and anti-estrogenic effects. However, to the best of our knowledge no information is available about this androgen steroid in relation to sperm quality. We analyzed 7beta-OH-EpiA in plasma and seminal plasma using a newly developed isotope dilution ultra-high performance liquid chromatography - mass spectrometry method. Validation met the requirements of FDA guidelines...
November 28, 2018: Physiological Research
Melissa Carvajal-Serna, Felipe Torres-Ruda, Jaime Antonio Cardozo, Henry Grajales-Lombana, José Álvaro Cebrián-Pérez, Teresa Muiño-Blanco, Rosaura Pérez-Pé, Adriana Casao
In temperate climates, photoperiod and melatonin regulate ram reproduction, modulating hormonal secretions, sperm quality, and seminal plasma composition. Information on the effect of an equatorial photoperiod (12L:12D) on ram reproduction, however, is scarce, and no data on hormonal concentrations and antioxidant enzyme activity in seminal plasma have been reported. Thus, the variation was investigated of melatonin and its relationship with testosterone and antioxidant enzyme activity in the seminal plasma of three sheep breeds in Colombia, when there was a consistent photoperiod during two dry and two rainy seasons per year...
November 16, 2018: Animal Reproduction Science
Claudia Fabrizio, Nicolò de Gennaro, Anna Volpe, Luigia Scudeller, Antonella Lagioia, Katia Falasca, Nicoletta Ladisa, Gioacchino Angarano, Laura Monno, Annalisa Saracino
BACKGROUND AND OBJECTIVES: We aimed to investigate to what extent a first-line DTG-based ART regimen reduces HIV-RNA in semen compared to plasma. STUDY DESIGN: In this prospective, observational study, ART-naïve, HIV-infected males starting their first ART regimen with DTG plus TDF/FTC or ABC/3TC were enrolled. Paired blood (BP) and seminal plasma (SP) samples were collected at baseline (T0) and at week-2/4/12/24 after ART initiation. Sexually transmitted infections (STI) were ruled out before enrolment...
November 15, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
Sharanbasappa Durg, Shivakumar Badamaranahalli Shivaram, Sachin Bavage
BACKGROUND: Withania somnifera Dunal, commonly known as Indian ginseng, has been in use since ancient times as anti-stress agent, aphrodisiac, for impotence and infertility treatment. PURPOSE: To evaluate the efficacy and safety of W. somnifera treatment in infertile men. STUDY DESIGN: An evidence-based systematic review and meta-analysis using Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines. METHODS: Published literature was searched in PubMed/MEDLINE, EMBASE, Scopus, the Cochrane Library, and DHARA...
November 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Peng Zhang, Xiao-Long Wang, Zhong-Hua Yang, Xin-Jun Su, Xing-Huan Wang
We aimed to establish a novel rat model of seminal vesiculitis that would provide an effective approach to investigate the pathogenesis of this disease in the future. Eight male rats received the same operation, during which the root of one of the two seminal vesicles was partly ligatured with sutures and the other vesicle was left intact. The samples of seminal vesicles were harvested on the 8th day following the operation. Hematoxylin and eosin and Masson's trichrome stains were used to observe the histopathology and the presence of fibrous tissue in seminal vesicles, respectively...
November 20, 2018: Asian Journal of Andrology
M G Ibrahim, E A Elghonaimy, S Schäfer, M Vennemann, S Kliesch, L Kiesel, M Götte, A N Schüring
PURPOSE: To study if short-term exposure (2 h and 6 h) of endometrial/endometriotic tissues and cells to 10% seminal plasma (SP) can induce EMT/metaplasia. METHODS: Basic research experimental study was carried out in a University hospital-based fertility center. Semen samples, peritoneal fluid (PF) from endometriosis patients, endometrial biopsy from premenopausal women, immortalized endometriotic epithelial cell line (12Z), and immortalized endometrial stromal cell line (St-T1b) were studied...
November 19, 2018: Archives of Gynecology and Obstetrics
Samantha M Calcatera, Darwin Reicks, Scott L Pratt
The objectives of this study were to identify and determine relative abundance of miRNAs in boar sperm, seminal plasma (SP), and serum pre- and post-viral infection. Functional enrichment analyses on predicted targets of miRNAs of interest were performed. Boars (n = 6) were inoculated with porcine reproductive and respiratory syndrome virus (PRRSv) strain 1-8-4 (Day 0). Semen and serum were collected on Day -2 and 6. Sperm and SP were separated and aliquots were flash frozen and stored at -80 °C. Serum was frozen and stored at -80 °C...
November 2, 2018: Animal Reproduction Science
Christina Schiza, Dimitrios Korbakis, Keith Jarvi, Eleftherios P Diamandis, Andrei P Drabovich
TEX101 is a germ cell-specific protein and a validated biomarker of male infertility. Mouse TEX101 was found essential for male fertility, and was suggested to function as a cell surface chaperone involved in maturation of proteins required for sperm migration and sperm-oocyte interaction. However, the precise functional role of human TEX101 is not known and cannot be studied in vitro due to the lack of human germ cell lines. Here, we genotyped 386 men for a common missense variant rs35033974 of TEX101, and identified 52 heterozygous and 4 homozygous men...
November 14, 2018: Molecular & Cellular Proteomics: MCP
Martin Schulze, Gábor Á Czirják, Karin Müller, Ralf Bortfeldt, Markus Jung, Ulrike Jakop
The aim of the present study was to characterize the lysozyme concentration and the bacterial killing activity (BKA) of boar seminal plasma against E. coli and S. aureus in 119 fertile Pietrain boars (aged: 18.1 ± 10.5 months). Lysozyme concentration was 2.4 ± 1.2 μg/ml in seminal plasma. More than 80% of the samples (97 of 119) showed BKA against E. coli or S. aureus or both bacterial strains: Group 1 (BKA against E. coli and S. aureus, n = 38), Group 2 (BKA against E. coli, n = 13), Group 3 (BKA against S...
November 3, 2018: Journal of Reproductive Immunology
Jiafeng Zhang, Ning Wang, Lin He, Xiaohong Pan, Xiaobei Ding
The alarming spread of HIV among men who have sex with men (MSM) has become a national concern in China. Estimating men's sexual HIV infectiousness from blood plasma viral load (BPVL) depends on the association between BPVL and semimal plasma viral load (SPVL). However, previous studies were controversial and few concentrated on MSM. Twenty antiretroviral therapy (ART) naive MSM and 54 MSM under ART were recruited between July and September 2015 in the city of Hangzhou, China. Blood and semen were collected in pairs at the same visit for each individual...
November 9, 2018: AIDS Research and Human Retroviruses
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