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Shin-Ichi Oikawa, Kiyoto Shiga, Daisuke Saito, Katsunori Katagiri, Aya Ikeda, Kodai Tuchida, Jun Miyaguchi, Kazuyuki Ishida, Tamotsu Sugai
The present study aimed to investigate the usefulness of contrast-enhanced ultrasonography (CEUS) and a newly developed analysis software for the detection of microcapillary network distribution in lymph nodes of patients with head and neck cancer (HNC) by comparing the CEUS and histopathological findings. Patients that were diagnosed with HNC between February and September 2016 were enrolled. A total of five patients underwent resection of the primary tumor and neck dissection as their initial treatment. The cervical lymph nodes of these patients were analyzed by CEUS intraoperatively, and their surgical specimens were examined histopathologically...
April 2018: Oncology Letters
Reza Khorramirouz, Jason L Go, Christopher Noble, Soumen Jana, Eva Maxson, Amir Lerman, Melissa D Young
OBJECTIVES: Subcutaneous implantations in small animal models are currently required for preclinical studies of acellular tissue to evaluate biocompatibility, including host recellularization and immunogenic reactivity. METHODS: Three rat subcutaneous implantation methods were evaluated in six Sprague Dawley rats. An acellular xenograft made from porcine pericardium was used as the tissue-scaffold. Three implantation methods were performed; 1) Suture method is where a tissue-scaffold was implanted by suturing its border to the external oblique muscle, 2) Control method is where a tissue-scaffold was implanted without any suturing or support, 3) Frame method is where a tissue-scaffold was attached to a circular frame composed of polycaprolactone (PCL) biomaterial and placed subcutaneously...
March 5, 2018: Acta Histochemica
Lilangi S Ediriwickrema, Michael Burnstine, Maria S Saber, Narsing Rao
Purpose: Primary malignant solitary fibrous tumor (SFT) of the orbit is a rare spindle cell neoplasm that requires excisional biopsy for histopathological diagnosis. We present a clinical case using contemporary immunohistochemical stains, report on the latest World Health Organization classification, and provide a review of the literature. Observations: Report of a single case of a 65 year old male who presented with right-sided proptosis, limited adduction, ptosis, lateral globe displacement, and cheek festooning...
April 2017: American Journal of Ophthalmology Case Reports
Muhammad Waqas, Waqas Rahim, Durre Shohab, Muhammad Athar Khawaja, Zafar Ali, Nadira Mamoon
Primary renal angiosarcoma is a rare tumor. Epithelioid variant of primary renal angiosarcoma is extremely rare and aggressive entity described in literature as a few case reports. It presents as solid looking renal mass as other renal cell carcinomas. Management is not well described due to scarcity of cases and aggressiveness of disease. We hereby report a case of epithelioid renal angiosarcoma in a 62-year female who presented with features of infected perinephric collection. Histopathological and immunohistochemical examinations of the renal specimen revealed lower polar renal tumor with atypical epithelioid cells having eccentric nuclei with coarse chromatin and eosinophilic cytoplasm, which were positive for vascular endothelial (FLI-1, CD 34, CD31 and factor VII) as well as epithelial (CKAE1/AE3) immune markers...
March 2018: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
Tianyue Pan, Zheng Wei, Yuan Fang, Zhihui Dong, Weiguo Fu
Early-phase clinical trials in patients with critical limb ischemia (CLI) have shown positive results of mononuclear cell therapy. The current meta-analysis investigated whether cluster of differentiation (CD) 34+ mononuclear cell therapy (CD34+ MCT) is effective for no-option CLI. Ten randomized controlled clinical studies of CD34+ MCT for no-option CLI with 479 patients were identified and analyzed for pooled results. Compared to control groups, the CD34+ MCT was associated with lower total amputation (odds ratio (OR): 0...
February 1, 2018: Vascular Medicine
Shuiling Jin, Yanfang Mi, Jing Song, Peipei Zhang, Yanyan Liu
Protein arginine methyltransferase 1 (PRMT1) serves pivotal roles in various cellular processes. However, its role in megakaryocytic differentiation has not been clearly reported. The aim of the present study was to explore the role of the PRMT-RNA binding motif protein 15 (RBM15) axis in human MK differentiation and the feasibility of targeting PRMT1 for leukemia treatment. In the present study, PRMT1 was overexpressed and the RBM15 protein was knocked down in human umbilical cord blood cluster of differentiation (CD)34+ cells and the cells were then cultured in megakaryocytic differentiation medium...
March 2018: Experimental and Therapeutic Medicine
Caiyun Shan, Yongsheng Ma
Stroke is the most common cause of mortality worldwide. Post-stroke angiogenesis is of great significance to the treatment of strokes. The aim of the present study was to investigate the mechanism underlying the angiogenesis-promoting effect of microRNA‑126 (miR‑126)‑associated signaling pathways using a stroke model in vivo and a cell migration model in vitro. Bone marrow‑derived endothelial progenitor cells (EPCs) were extracted and identified using a density gradient method. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed to examine the expression levels of miR‑126 and C‑X‑C chemokine receptor type 7 (CXCR7)...
January 29, 2018: Molecular Medicine Reports
Yingming Xiang, Shaosong Tu, Fangbiao Zhang
RATIONALE: Mediastinal solitary fibrous tumors (SFTs) are rare mesenchymal neoplasms. Complete resection is considered as the effective treatment and the prognosis is quite good. Rapid metastasis after surgery is extremely rare. PATIENT CONCERNS: In this case report we describe a 42-year-old man who present with a mediastinal malignant SFTs. Enhanced computed tomography of chest revealed a 4.5 × 4.0-cm mass in the anterior mediastinum. DIAGNOSES: The tumor is composed of massive proliferation of atypical spindle cells...
December 2017: Medicine (Baltimore)
Karam Kim, Sae Mi Hwang, Sung Min Kim, Sung Woo Park, Yunjae Jung, Il Yup Chung
Neutrophils and eosinophils, 2 prominent granulocytes, are commonly derived from myelocytic progenitors through successive stages in the bone marrow. Our previous genome-wide transcriptomic data unexpectedly showed that genes encoding a multitude of neutrophil primary granule proteins (NPGPs) were markedly downregulated during the end period of eosinophilic terminal differentiation when cord blood (CB) cluster of differentiation (CD) 34+ cells were induced to differentiate toward the eosinophil lineage during a 24-day culture period...
December 2017: Immune Network
Sarita Rani Jaiswal, Prakash Bhakuni, Aby Joy, Nisha Murli, Priyanka Bharadwaj, Shamsur Zaman, Murugayan Nedunchezian, Suparno Chakrabarti
We conducted a prospective study on T and natural killer (NK) cell subset composition of graft and transplant outcomes in T cell-replete haploidentical transplantation with a single dose of subcutaneous plerixafor (Px) added to granulocyte colony-stimulating factor (G-CSF)-based mobilization in allogeneic donors to collect 10 × 106 /kg CD34+ hematopoietic stem cells (HSCs) at single apheresis. Twnety-six donors received G-CSF + Px and 25 G-CSF alone for mobilization. Despite significantly lower peripheral blood (PB) CD34+ HSCs on day 4 in the G-CSF + Px group (33 [range, 6-47] cells/µL versus 81 [range, 50-168] cells/µL in the G-CSF group; P = ...
March 2018: Biology of Blood and Marrow Transplantation
Fergun Yilmaz, Nur Soyer, Ruchan Uslu, Mahmut Tobu, Burcak Karaca, Guray Saydam, Fahri Sahin, Filiz Vural
BACKGROUND: In the literature, risk factors for poor mobilization were tried to identify. However, most of the studies consisted heterogeneous group of patients including both hematologic and oncologic malignancies. In this study, we aimed to identify the risk factors for poor mobilization in adults with solid tumors. METHODS: We enrolled 49(47 men, 2 women) adult patients with solid tumor who were mobilized between September 2007 and February 2017. All the mobilization procedures were performed with G-CSF(10μg/kg/day) with chemotherapy...
December 2017: Transfusion and Apheresis Science
Poliana Ramos Braga Santos, Claúdia Malheiros Coutinho-Camillo, Fernando Augusto Soares, Valéria Souza Freitas, Deise Souza Vilas-Bôas, Flávia Caló Aquino Xavier, Clarissa Araújo Gurgel Rocha, Iguaracyra Barreto de Araújo, Jean Nunes Dos Santos
The aim of this study was evaluate the expression profile of microRNAs related to mast cells activation and angiogenesis in salivary glands tumors. METHOD: We have analyzed the expression of miR-9, miR-16, miR-17, miR-132, miR-195 and miR-221 by real-time RT-PCR, in 11 adenoid cystic carcinomas, 9 mucoepidermoid carcinomas and 11 pleomorphic adenomas. Immunohistochemical investigation was performed to detect mast cells tryptase and CD-34 for microvessels biomarkers. miR-16, miR-17, miR-132, miR-195 and miR-221 showed a decreased expression, whereas miR-9 showed an increased expression in most cases compared to normal tissues...
October 18, 2017: Pathology, Research and Practice
Liping Zhou, Xiaowei Zhang, Panpan Zhou, Xue Li, Xuejing Xu, Qing Shi, Dong Li, Xiuli Ju
Successfully expanding hematopoietic stem cells (HSCs) is advantageous for clinical HSC transplantation. The present study investigated the influence of testosterone on the proliferation, antigen phenotype and expression of hematopoiesis-related genes in umbilical cord blood-derived cluster of differentiation (CD)34(+) cells under normoxic or hypoxia conditions. Cord blood (CB) CD34(+) cells were separated using magnetic activated cell sorting. A cytokine cocktail and feeder cells were used to stimulate the expansion of CD34(+) cells under normoxic (20% O2) and hypoxic (1% O2) conditions for 7 days and testosterone was added accordingly...
November 2017: Experimental and Therapeutic Medicine
Yong Liu, Bo Wei, Xuebing Zhang, Dehui Xu, Bo Wang, Guochao Yin, Dawer Gu, Yuxiang Li, Daliang Kong
The aim of the present study was to identify potential therapeutic target genes and miRNAs for primary myelofibrosis (PMF). The dataset GSE53482 was downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) of peripheral blood (PB) cluster of differentiation (CD)34(+) cells from PMF patients (PB-PMF group) and peripheral blood CD34(+) cells from healthy individuals (PB-control group) were analyzed using the Linear Models for Microarray Data package in R...
October 2017: Experimental and Therapeutic Medicine
Gunjan L Shah, Michael Scordo, Satyajit Kosuri, Diego Adrianzen Herrera, Christina Cho, Sean M Devlin, Taylor Borrill, Dean C Carlow, Scott T Avecilla, Richard C Meagher, Richard J O'Reilly, Ann A Jakubowski, Esperanza B Papadopoulos, Guenther Koehne, Boglarka Gyurkocza, Hugo Castro-Malaspina, Brian C Shaffer, Miguel-Angel Perales, Sergio A Giralt, Roni Tamari
Ex vivo CD34(+) selection before allogeneic hematopoietic stem cell transplantation (allo-HCT) reduces graft-versus-host disease without increasing relapse but usually requires myeloablative conditioning. We aimed to identify toxicity patterns in older patients and the association with overall survival (OS) and nonrelapse mortality (NRM). We conducted a retrospective analysis of 200 patients who underwent CD34(+) selection allo-HCT using the ClinicMACS system between 2006 and 2012. All grade 3 to 5 toxicities by CTCAE v4...
September 22, 2017: Biology of Blood and Marrow Transplantation
Kensaku Yamaga, Akihiro Fujita, Mari Osaki, Satoshi Kuwamoto, Naoko Ishiguro, Tadahito Yamamoto, Hideki Nagashima
Superficial cluster of differentiation (CD)34-positive fibroblastic tumor (SCPFT) is a rare mesenchymal neoplasm of borderline malignancy. It is characterized by a superficial location, marked cellular pleomorphism, an extremely low incidence of mitotic figures, and strong CD34 immunohistochemical positivity. As SCPFT is a recently described neoplasm, its characteristics are yet to be fully elucidated. To the best of our knowledge, no detailed studies regarding the imaging findings and cytogenetic analyses of SCPFTs exist...
September 2017: Oncology Letters
Sugathan Subi, Sungjin Lee, Supriya Shiwani, Naresh Kumar Singh
Objective: Myogenic satellite cells were isolated from semitendinosus muscle of prepubertal Korean black goat to observe the differential effect of linolenic and retinoic acid in thepresence of thiazolidinediones and also to observe the production insulin sensitive preadipocyte. Methods: Cells were characterized for its stemness with CD 34, CD 13, CD 106, CD44, Vimentin surface markers using flow cytometry. Cells characterized themselves to possess significant (p<0...
September 18, 2017: Asian-Australasian Journal of Animal Sciences
Md Shahid Alam, Bipasha Mukherjee, S Krishnakumar, Jyotirmay Biswas
Perivascular epithelioid cell tumor (PEComa) is a rare neoplasm considered to arise from myomelanocytic cell lineage. The uterus is reportedly the most common site to be involved. Orbital PEComa is extremely rare with only two cases reported till date. A 5-year-old male presented with a right medial orbital mass for the last 6 months. The patient was diagnosed with alveolar soft part sarcoma elsewhere. Magnetic resonance imaging features were suggestive of lymphangioma with bleeding. The excision biopsy revealed multiple tumor cells comprising epithelioid cells with clear cytoplasm, along with nuclear atypia and mitosis...
September 2017: Indian Journal of Ophthalmology
Hyun Jin Min, Kyung Soo Kim
Recently, as the authors experienced nasal septal schwannoma and nasal septal neurofibroma with similar clinical symptoms but different endoscopic findings, the authors tried to review all the literatures previously reported on the "Nasal septal schwannoma and Nasal septal neurofibroma." The aim of this study is to thoroughly review previously reported patients with nasal septal schwannomas and neurofibromas and to describe similar and different features focusing on the differential diagnosis between the 2 entities...
October 2017: Journal of Craniofacial Surgery
Canan Sadullahoğlu, Yelda Dere, Türkan Rezanko Atasever, Mine Tunakan Öztop, Önder Karaaslan
OBJECTIVE: Dermatofibroma (DF) is a benign fibrohistiocytic tumor whereas dermatofibrosarcoma protuberans (DFSP) has intermediate malignant potential. CD34 is the most commonly used antibody in differentiating these tumors. Various studies have stated the rates of D2-40 expression as 0-50% in DFSPs and 86-100% in DFs. Our aim in this study was to determine the expression of CD34 and D2-40 in DFs and DFSPs and the possible use of D2-40 in the differential diagnosis of these lesions. MATERIAL AND METHOD: This is a retrospective study including 30 DF and 15 DFSP cases which were reevaluated for epidermal changes, the presence of a transmission zone (Grenz zone), infiltration of soft tissues, infiltration pattern and histologic subtypes in addition to cellular pleomorphism, nuclear atypia, and necrosis...
2017: Türk Patoloji Dergisi
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