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https://www.readbyqxmd.com/read/28343243/fusarium-species-a-promising-tool-box-for-industrial-biotechnology
#1
REVIEW
Marina Gabriel Pessôa, Bruno Nicolau Paulino, Mario Cezar Rodrigues Mano, Iramaia Angélica Neri-Numa, Gustavo Molina, Glaucia Maria Pastore
Global demand for biotechnological products has increased steadily over the years. Thus, need for optimized processes and reduced costs appear as a key factor in the success of this market. A process tool of high importance is the direct or indirect use of enzymes to catalyze the generation of various substances. Also, obtaining aromas and pigments from natural sources has becoming priority in cosmetic and food industries in order to supply the demand from consumers to substitute synthetic compounds, especially when by-products can be used as starting material for this purpose...
March 25, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28342313/the-glyoxylate-pathway-contributes-to-enhanced-extracellular-electron-transfer-in-yeast-based-biofuel-cell
#2
Yolina Hubenova, Eleonora Hubenova, Evelina Slavcheva, Mario Mitov
This study provides a new insight into our understanding of yeast response to starvation conditions (sole acetate as carbon source) and applied polarization and offers important information about the role of the glyoxylate cycle in the carbohydrate synthesis and extracellular charge transfer processes in biofuel cells. The biosynthetic capabilities of yeast C. melibiosica 2491 and the up/down-regulation of the glyoxylate cycle are evaluated by modifying the cellular metabolism by feedback inhibition or carbohydrate presence and establishing the malate dehydrogenase activity and carbohydrate content together with the electric charge passed through bioelectrochemical system...
March 14, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28340437/assessment-of-microalga-biofilms-for-simultaneous-remediation-and-biofuel-generation-in-mine-tailings-water
#3
H Palma, E Killoran, M Sheehan, F Berner, K Heimann
Microalgae crops can generate a biochemical profile of high energy density and may be used for remediation of contaminated waste waters. This manuscript presents a laboratory-scale investigation into the potential for growing endemic microalgae biofilms in phosphorus-enriched nickel refinery tailings water, with an emphasis on product potential and the remediation of heavy metals. The dominant species of the consortia was a Chlorella-like microalga. The growth was monitored over time, with a productivity (0...
March 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28338601/mycotoxin-biotransformation-by-native-and-commercial-enzymes-present-and-future-perspectives
#4
REVIEW
Martina Loi, Francesca Fanelli, Vania C Liuzzi, Antonio F Logrieco, Giuseppina Mulè
Worldwide mycotoxins contamination has a significant impact on animal and human health, and leads to economic losses accounted for billions of dollars annually. Since the application of pre- and post- harvest strategies, including chemical or physical removal, are not sufficiently effective, biological transformation is considered the most promising yet challenging approach to reduce mycotoxins accumulation. Although several microorganisms were reported to degrade mycotoxins, only a few enzymes have been identified, purified and characterized for this activity...
March 24, 2017: Toxins
https://www.readbyqxmd.com/read/28337906/fates-of-chemical-elements-in-biomass-during-its-pyrolysis
#5
Wu-Jun Liu, Wen-Wei Li, Hong Jiang, Han-Qing Yu
Biomass is increasingly perceived as a renewable resource rather than as an organic solid waste today, as it can be converted to various chemicals, biofuels, and solid biochar using modern processes. In the past few years, pyrolysis has attracted growing interest as a promising versatile platform to convert biomass into valuable resources. However, an efficient and selective conversion process is still difficult to be realized due to the complex nature of biomass, which usually makes the products complicated...
March 24, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28337840/electrogenic-single-species-biocomposites-as-anodes-for-microbial-fuel-cells
#6
Patrick Kaiser, Steffen Reich, Daniel Leykam, Monika Willert-Porada, Andreas Greiner, Ruth Freitag
Integration of electrogenic microorganisms remains a challenge in biofuel cell technology. Here, synthetic biocomposites ("artificial biofilms") are proposed. Bacteria (Shewanella oneidensis) are embedded in a hydrogel matrix (poly(vinyl alcohol)) via wet- and electrospinning, creating fibers and nonwoven gauzes. The bacteria remain viable and metabolically active. The performance is compared to S. oneidensis suspension cultures and "natural" biofilms. While lower than with the suspension cultures, the power output from the fuel cells with the artificial biofilms is higher than with the natural one...
March 24, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28337710/high-efficient-n-butanol-production-by-co-culturing-clostridium-acetobutylicum-and-saccharomyces-cerevisiae-integrated-with-butyrate-fermentative-supernatant-addition
#7
Hongzhen Luo, Qingwei Zeng, Shuo Han, Zhaoyu Wang, Qing Dong, Yanhong Bi, Yuping Zhao
Butanol is not only an important chemical intermediate and solvent in pharmaceutical and cosmetics industries, but also considered as an advanced biofuel. Although species of the natural host Clostridium have been engineered, butanol titers in the anaerobe seem to be limited by its intolerance to butanol less than 13 g/L. Here we aimed to develop a technology for enhancing butanol production by a co-culture system with butyrate fermentative supernatant addition. First, when adding 4.0 g/L butyrate into the acetone-butanol-ethanol (ABE) fermentation broth with single-shot at 24 h, the "acid crash" phenomenon occurred and the ABE fermentation performance deteriorated...
April 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28335611/evaluation-of-thioesterases-from-acinetobacter-baylyi-for-production-of-free-fatty-acids
#8
Rahul Ukey, William E Holmes, Rakesh Bajpai, Andrei Y Chistoserdov
Acinetobacter baylyi is one of few Gram-negative bacteria capable of accumulating storage lipids in the form of triacylglycerides and wax esters, which makes it an attractive candidate for production of lipophilic products, including biofuel precursors. Thioesterases play a significant dual role in the triacylglyceride and wax ester biosynthesis by either providing or removing acyl-CoA from this pathway. Therefore, 4 different thioesterase genes were cloned from Acinetobacter baylyi ADP1 and expressed in Escherichia coli to investigate their contribution to free fatty acids (FFAs) accumulation...
March 23, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28335589/global-functional-analysis-of-butanol-sensitive-escherichia-coli-and-its-evolved-butanol-tolerant-strain
#9
Haeyoung Jeong, Seung-Won Lee, Sun Hong Kim, Eun-Youn Kim, Sinyeon Kim, Sung Ho Yoon
Butanol is a promising alternative to ethanol and is desirable for use in transportation fuels and additives to gasoline and diesel fuels. Microbial production of butanol is challenging primarily due to its toxicity and low titer of production. Herein, we compared the transcriptome and phenome of wild-type Escherichia coli and its butanol-tolerant evolved strain to understand the global cellular physiology and metabolism responsible for butanol tolerance. When the ancestral butanol-sensitive E. coli was exposed to butanol, gene activities involved in respiratory mechanisms and oxidative stress were highly perturbed...
March 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28335483/land-use-for-edible-protein-of-animal-origin-a-review
#10
REVIEW
Gerhard Flachowsky, Ulrich Meyer, Karl-Heinz Südekum
The present period is characterized by a growing world population and a higher demand for more and better quality food, as well as other products for an improved standard of living. In the future, there will be increasingly strong competition for arable land and non-renewable resources such as fossil carbon-sources, water, and some minerals, as well as between food, feed, fuel, fiber, flowers, and fun (6 F's). Proteins of animal origin like milk, meat, fish, eggs and, probably, insects are very valuable sources of essential amino acids, minerals and vitamins, but their production consumes some non-renewable resources including arable land and causes considerable emissions...
March 18, 2017: Animals: An Open Access Journal From MDPI
https://www.readbyqxmd.com/read/28332296/electrochemistry-for-the-generation-of-renewable-chemicals-one-pot-electrochemical-deoxygenation-of-xylose-to-%C3%AE-valerolactone
#11
Olusola Oladele James, Waldemer Sauter, Uwe Schröder
In this study, we demonstrate the electrochemical conversion of xylose to δ-valerolactone via carbonyl intermediates. The conversion was achieved in aqueous media and at ambient conditions. This study also demonstrates that feedstocks for the use of electrochemistry for production of renewable chemicals and biofuels can be extended to primary carbohydrate molecules. This is the first report on a one-pot electrochemical deoxygenation of xylose to δ-valerolactone.
March 22, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28331945/from-a-metagenomic-source-to-a-high-resolution-structure-of-a-novel-alkaline-esterase
#12
Mariana Rangel Pereira, Thaís Carvalho Maester, Gustavo Fernando Mercaldi, Eliana Gertrudes de Macedo Lemos, Marko Hyvönen, Andrea Balan
Esterases catalyze the cleavage and formation of ester bonds and are members of the diverse family of α/β hydrolase fold. They are useful in industries from different sectors, such as food, detergent, fine chemicals, and biofuel production. In a previous work, 30 positive clones for lipolytic activity were identified from a metagenomic library of a microbial consortium specialized in diesel oil degradation. In this study, a putative gene encoding an esterase/lipase, denominated est8, has been cloned and the corresponding protein expressed recombinantly, purified to homogeneity and characterized functional and structurally...
March 22, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28331545/metabolic-engineering-of-saccharomyces-cerevisiae-to-produce-a-reduced-viscosity-oil-from-lignocellulose
#13
Tam N T Tran, Rebecca J Breuer, Ragothaman Avanasi Narasimhan, Lucas S Parreiras, Yaoping Zhang, Trey K Sato, Timothy P Durrett
BACKGROUND: Acetyl-triacylglycerols (acetyl-TAGs) are unusual triacylglycerol (TAG) molecules that contain an sn-3 acetate group. Compared to typical triacylglycerol molecules (here referred to as long chain TAGs; lcTAGs), acetyl-TAGs possess reduced viscosity and improved cold temperature properties, which may allow direct use as a drop-in diesel fuel. Their different chemical and physical properties also make acetyl-TAGs useful for other applications such as lubricants and plasticizers...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28331544/vapor-fed-bio-hybrid-fuel-cell
#14
Marcus S Benyamin, Justin P Jahnke, David M Mackie
BACKGROUND: Concentration and purification of ethanol and other biofuels from fermentations are energy-intensive processes, with amplified costs at smaller scales. To circumvent the need for these processes, and to potentially reduce transportation costs as well, we have previously investigated bio-hybrid fuel cells (FCs), in which a fermentation and FC are closely coupled. However, long-term operation requires strictly preventing the fermentation and FC from harming each other. We introduce here the concept of the vapor-fed bio-hybrid FC as a means of continuously extracting power from ongoing fermentations at ambient conditions...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28330553/physiological-and-molecular-insights-into-the-high-salinity-tolerance-of-pongamia-pinnata-l-pierre-a-potential-biofuel-tree-species
#15
Sureshbabu Marriboina, Debashree Sengupta, Sumit Kumar, Attipalli R Reddy
Soil salinity is gradually becoming a threat to the global economy by affecting agricultural productivity worldwide. Here, we analyze the salinity tolerance of Pongamia pinnata with an insight into the underlying physiological and molecular responses. Despite a reduction in net photosynthetic rate, P. pinnata efficiently maintained its leaf water potentials even at 500mM NaCl for 15days and displayed no visible stress symptoms. Na(+) localization analysis using CoroNa-Green AM revealed effective Na(+) sequestration in the roots when compared to leaves...
May 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28330241/purification-characterization-gene-cloning-and-expression-of-gh-10-xylanase-penicillium-citrinum-isolate-hzn13
#16
Zabin K Bagewadi, Sikandar I Mulla, Harichandra Z Ninnekar
An extracellular thermostable xylanase (Xyl-IIb) produced by Penicillium citrinum isolate HZN13 was purified to homogeneity using DEAE-Sepharose, Sephadex G-100 and Bio-Gel P-60 chromatography with specific activity of 6272.7 U/mg and 19.6-fold purification. The purification revealed the occurrence of multiple forms of xylanases (Xyl-I, Xyl-IIa, Xyl-IIb and Xyl-III). The molecular mass of highly purified Xyl-IIb was ~31 kDa with SDS-PAGE. The enzyme was cellulase-free, thermostable (55-75 °C) and acidophilic (3...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330202/impact-of-various-media-and-organic-carbon-sources-on-biofuel-production-potential-from-chlorella-spp
#17
Amit Kumar Sharma, Pradeepta Kumar Sahoo, Shailey Singhal, Alok Patel
In this study, five Chlorella species (Chlorella vulgaris, Chlorella minutissima, Chlorella pyrenoidosa, Chlorella sp.1 and Chlorella sp.2) were grown in various nutrient medium including BG-11, BBM, Fog's medium and M4N medium for the evolution of biomass and lipid production potential. Among the tested medium, BG-11 was found most economical and efficient medium for all Chlorella species. To see the impact of organic carbon sources on lipid production potential, all microalgae species were also cultured in selected medium (BG-11) with different organic carbon sources like glucose, glycerol, sodium acetate, and sucrose under mixotrophic condition...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330171/purification-and-characterization-of-endo-%C3%AE-1-4-d-glucanase-from-trichoderma-harzianum-strain-hzn11-and-its-application-in-production-of-bioethanol-from-sweet-sorghum-bagasse
#18
Zabin K Bagewadi, Sikandar I Mulla, Harichandra Z Ninnekar
An acidophilic-solvent-thermostable endo β-1,4-D-glucanase produced from a potential Trichoderma harzianum strain HZN11 was purified to homogeneity by DEAE-Sepharose and Sephadex G-100 chromatography with 33.12 fold purification with specific activity of 66.25 U/mg and molecular mass of ~55 kDa. The optimum temperature and pH were 60 °C and 5.5 retaining 76 and 85 % of activity after 3 h, respectively. It showed stability between pH 4.5-6.0 and temperature between 50-70 °C indicating thermostability...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330139/production-of-bioethanol-as-useful-biofuel-through-the-bioconversion-of-water-hyacinth-eichhornia-crassipes
#19
Arpan Das, Priyanka Ghosh, Tanmay Paul, Uma Ghosh, Bikas Ranjan Pati, Keshab Chandra Mondal
Water hyacinth (Eichhornia crassipes) represents a promising candidate for fuel ethanol production in tropical countries because of their high availability and high biomass yield. Bioconversion of such biomass to bioethanol could be wisely managed through proper technological approach. In this work, pretreatment of water hyacinth (10 %, w/v) with dilute sulfuric acid (2 %, v/v) at high temperature and pressure was integrated in the simulation and economic assessment of the process for further enzymatic saccharification was studied...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330126/characterization-of-lignin-degrading-enzymes-ldes-from-a-dimorphic-novel-fungus-and-identification-of-products-of-enzymatic-breakdown-of-lignin
#20
Lipin Dev Mundur Sahadevan, Chandra Shekhar Misra, V Thankamani
Lignin is a major component of all plants, the degradation of which remains a major challenge to date owing to its recalcitrant nature. Several classes of fungi have been studied to carry out this process to some extent, but overall the process remains inefficient. We have isolated a novel alkalophilic dimorphic lignin-degrading Deuteromycete from soil, identified as "uncultured" and coded as MVI.2011. Supernatant from 12-h culture of MVI.2011 in optimized mineral medium containing lignin pH 9.0 was analysed for Lignin Peroxidase, Manganese Peroxidase and Laccase...
June 2016: 3 Biotech
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