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https://www.readbyqxmd.com/read/28439814/imaging-the-accumulated-intracellular-microalgal-lipids-as-a-response-to-temperature-stress
#1
Khaled N M Elsayed, Tatiana A Kolesnikova, Anja Noke, Gerd Klöck
Over the last few decades, many scientists considered microalgae as promising actors for future biofuels because of the high lipid productivity inside their cells. Moreover, much attention has been paid to algal lipids as they can be used in biodiesel production. In this study, we optimized the different suitable conditions such as incubation time, incubation temperature, Dimethylesulfoxide and Nile red concentrations of the lipophilic fluorescence dye Nile red as an excellent and fast vital stain to detect and quantify intracellular lipids...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28439415/stretchable-biofuel-cells-as-wearable-textile-based-self-powered-sensors
#2
Itthipon Jeerapan, Juliane R Sempionatto, Adriana Pavinatto, Jung-Min You, Joseph Wang
Highly stretchable textile-based biofuel cells (BFCs), acting as effective self-powered sensors, have been fabricated using screen-printing of customized stress-enduring inks. Due to synergistic effects of nanomaterial-based engineered inks and the serpentine designs, these printable bioelectronic devices endure severe mechanical deformations, e.g., stretching, indentation, or torsional twisting. Glucose and lactate BFCs with the single enzyme and membrane-free configurations generated the maximum power density of 160 and 250 µW cm(-2) with the open circuit voltages of 0...
December 21, 2016: Journal of Materials Chemistry. A, Materials for Energy and Sustainability
https://www.readbyqxmd.com/read/28439294/impact-of-lignin-polymer-backbone-esters-on-ionic-liquid-pretreatment-of-poplar
#3
Kwang Ho Kim, Tanmoy Dutta, John Ralph, Shawn D Mansfield, Blake A Simmons, Seema Singh
BACKGROUND: Biomass pretreatment remains an essential step in lignocellulosic biofuel production, largely to facilitate the efficient removal of lignin and increase enzyme accessibility to the polysaccharides. In recent years, there have been significant efforts in planta to reduce lignin content or modify its composition to overcome the inherent recalcitrance that it imposes on lignocellulosic biomass during processing. Here, transgenic poplar lines in which monolignol ferulate conjugates were synthesized during cell wall development to introduce, during lignification, readily cleavable ester linkages into the lignin polymer backbone (i...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28436519/a-ruthenium-ii-bis-phosphinophosphinine-complex-as-a-precatalyst-for-transfer-hydrogenation-and-hydrogen-borrowing-reactions
#4
R J Newland, M F Wyatt, R L Wingad, S M Mansell
The 2-phosphinophosphinine 2-PPh2-3-Me-6-SiMe3-PC5H2 (2) has been prepared and was shown to act as a κ(2)-chelating ligand in cis-[RuCl2(2)2] (4). Complex 4 was a competent precatalyst for the room temperature transfer hydrogenation of acetophenone (0.1 mol% 4 and 0.5 mol% KO(t)Bu) and the conversion of methanol/ethanol mixtures to the advanced biofuel isobutanol in 50% yield and 96% selectivity.
April 24, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28436149/transgenic-mir156-switchgrass-in-the-field-growth-recalcitrance-and-rust-susceptibility
#5
Holly L Baxter, Mitra Mazarei, Alexandru Dumitrache, Jace Natzke, Miguel Rodriguez, Jiqing Gou, Chunxiang Fu, Robert W Sykes, Geoffrey B Turner, Mark F Davis, Steven Brown, Brian Davison, Zeng-Yu Wang, C Neal Stewart
Sustainable utilization of lignocellulosic perennial grass feedstocks will be enabled by high biomass production and optimized cell wall chemistry for efficient conversion into biofuels. MicroRNAs are regulatory elements that modulate the expression of genes involved in various biological functions in plants, including growth and development. In greenhouse studies, overexpressing a microRNA (miR156) gene in switchgrass had dramatic effects on plant architecture and flowering, which appeared to be driven by transgene expression levels...
April 24, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28435444/role-of-trichoderma-reesei-mitogen-activated-protein-kinases-mapks-in-cellulase-formation
#6
Mingyu Wang, Meiling Zhang, Ling Li, Yanmei Dong, Yi Jiang, Kuimei Liu, Ruiqin Zhang, Baojie Jiang, Kangle Niu, Xu Fang
BACKGROUND: Despite being the most important cellulase producer, the cellulase-regulating carbon source signal transduction processes in Trichoderma reesei are largely unknown. Elucidating these processes is the key for unveiling how external carbon sources regulate cellulase formation, and ultimately for the improvement of cellulase production and biofuel production from lignocellulose. RESULTS: In this work, the role of the mitogen-activated protein kinase (MAPK) signal transduction pathways on cellulase formation was investigated...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28435443/occurrence-of-plastidial-triacylglycerol-synthesis-and-the-potential-regulatory-role-of-agpat-in-the-model-diatom-phaeodactylum-tricornutum
#7
Srinivasan Balamurugan, Xiang Wang, Hong-Lei Wang, Chun-Jing An, Hui Li, Da-Wei Li, Wei-Dong Yang, Jie-Sheng Liu, Hong-Ye Li
BACKGROUND: Microalgae have emerged as a potential feedstock for biofuels and bioactive components. However, lack of microalgal strains with promising triacylglycerol (TAG) content and desirable fatty acid composition have hindered its commercial feasibility. Attempts on lipid overproduction by metabolic engineering remain largely challenging in microalgae. RESULTS: In this study, a microalgal 1-acyl-sn-glycerol-3-phosphate acyltransferase designated AGPAT1 was identified in the model diatom Phaeodactylum tricornutum...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28433899/sustainable-production-of-a-new-generation-biofuel-by-lipase-catalyzed-esterification-of-fatty-acids-from-liquid-industrial-waste-biomass
#8
Athanasios Foukis, Olga A Gkini, Panagiota-Yiolanda Stergiou, Vasilios A Sakkas, Agapi Dima, Konstantina Boura, Athanasios Koutinas, Emmanuel M Papamichael
In this work we suggest a methodology comprising the design and use of cost-effective, sustainable, and environmentally friendly process for biofuel production compatible with the market demands. A new generation biofuel is produced using fatty acids, which were generated from acidogenesis of industrial wastes of bioethanol distilleries, and esterified with selected alcohols by immobilized Candida antarctica Lipase-B. Suitable reactors with significant parameters and conditions were studied through experimental design, and novel esterification processes were suggested; among others, the continuous removal of the produced water was provided...
April 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28433683/municipal-wastewater-sludge-as-a-sustainable-bioresource-in-the-united-states
#9
Timothy E Seiple, André M Coleman, Richard L Skaggs
Within the United States and Puerto Rico, publicly owned treatment works (POTWs) process 130.5 Gl/d (34.5 Bgal/d) of wastewater, producing sludge as a waste product. Emerging technologies offer novel waste-to-energy pathways through whole sludge conversion into biofuels. Assessing the feasibility, scalability and tradeoffs of various energy conversion pathways is difficult in the absence of highly spatially resolved estimates of sludge production. In this study, average wastewater solids concentrations and removal rates, and site specific daily average influent flow are used to estimate site specific annual sludge production on a dry weight basis for >15,000 POTWs...
April 20, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28433585/scale-up-for-esters-production-from-straw-whiskers-for-biofuel-applications
#10
Agapi Dima, Konstantina Boura, Argyro Bekatorou, Panagiota-Yiolanda Stergiou, Athanasios Foukis, Olga A Gkini, Panagiotis Kandylis, Katerina Pissaridi, Maria Kanellaki, Emmanuel M Papamichael, Athanasios A Koutinas
Delignified wheat straw was fermented by a mixed bacterial anaerobic culture obtained from a UASB reactor to produce organic acids (OAs). Kissiris was used as immobilization carrier in a 2-compartment 82L bioreactor filled with 17L of fermentation broth for the first 7 fermentation batches and up to 40L for the subsequent batches. The amount of straw used was 30g/L and the temperature was set at 37°C for all experiments. The total OAs reached concentrations up to 17.53g/L and the produced ethanol ranged from 0...
April 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28432948/butanol-fermentation-of-the-brown-seaweed-laminaria-digitata-by-clostridium-beijerinckii-dsm-6422
#11
Xiaoru Hou, Nikolaj From, Irini Angelidaki, Wouter J J Huijgen, Anne-Belinda Bjerre
Seaweed represents an abundant, renewable, and fast-growing biomass resource for 3rd generation biofuel production. This study reports an efficient butanol fermentation process carried out by Clostridium beijerinckii DSM-6422 using enzymatic hydrolysate of the sugar-rich brown seaweed Laminaria digitata harvested from the coast of the Danish North Sea as substrate. The highest butanol yield (0.42g/g-consumed-substrates) compared to literature was achieved, with a significantly higher butanol:acetone-butanol-ethanol (ABE) molar ratio (0...
April 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28432390/biomass-resources-agriculture
#12
Ingeborg N Kluts, Marnix L J Brinkman, Sierk A de Jong, H Martin Junginger
Bioenergy is the single largest source of renewable energy in the European Union (EU-28); of this, 14% was produced from agricultural feedstocks in 2012. This chapter provides an overview of the current use (for bioenergy) and future potential of agricultural feedstocks for (amongst others) biorefinery purposes in the European Union. The main application of these feedstocks is currently the production of biofuels for road transport. Biodiesel makes up 80% of the European biofuel production, mainly from rapeseed oil, and the remaining part is bioethanol from wheat and sugar beet...
April 22, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28432100/casein-kinase-i-isoform-hrr25-is-a-negative-regulator-of-haa1-in-the-weak-acid-stress-response-pathway-in-saccharomyces-cerevisiae
#13
Morgan E Collins, Joshua J Black, Zhengchang Liu
Haa1 is a transcription factor that adapts Saccharomyces cerevisiae cells to weak organic acid stresses by activating the expression of various genes. Many of these genes encode membrane proteins such as TPO2 and YRO2 How Haa1 is activated by weak acids is not clear. Here, we show that casein kinase I isoform Hrr25 is an important negative regulator of Haa1. Haa1 is known to be multiply phosphorylated. We found that mutations in HRR25 lead to reduced Haa1 phosphorylation and increased expression of Haa1-target genes, and that Hrr25 interacts with Haa1...
April 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28429326/wax-ester-fermentation-and-its-application-for-biofuel-production
#14
Hiroshi Inui, Takahiro Ishikawa, Masahiro Tamoi
In Euglena cells under anaerobic conditions, paramylon, the storage polysaccharide, is promptly degraded and converted to wax esters. The wax esters synthesized are composed of saturated fatty acids and alcohols with chain lengths of 10-18, and the major constituents are myristic acid and myristyl alcohol. Since the anaerobic cells gain ATP through the conversion of paramylon to wax esters, the phenomenon is named "wax ester fermentation". The wax ester fermentation is quite unique in that the end products, i...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28428822/in-muro-deacetylation-of-xylan-affects-lignin-properties-and-improves-saccharification-of-aspen-wood
#15
Prashant Mohan-Anupama Pawar, Marta Derba-Maceluch, Sun-Li Chong, Madhavi Latha Gandla, Shamrat Shafiul Bashar, Tobias Sparrman, Patrik Ahvenainen, Mattias Hedenström, Merve Özparpucu, Markus Rüggeberg, Ritva Serimaa, Martin Lawoko, Maija Tenkanen, Leif J Jönsson, Ewa J Mellerowicz
BACKGROUND: Lignocellulose from fast growing hardwood species is a preferred source of polysaccharides for advanced biofuels and "green" chemicals. However, the extensive acetylation of hardwood xylan hinders lignocellulose saccharification by obstructing enzymatic xylan hydrolysis and causing inhibitory acetic acid concentrations during microbial sugar fermentation. To optimize lignocellulose for cost-effective saccharification and biofuel production, an acetyl xylan esterase AnAXE1 from Aspergillus niger was introduced into aspen and targeted to cell walls...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28428820/regional-water-footprints-of-potential-biofuel-production-in-china
#16
Xiaomin Xie, Tingting Zhang, Liming Wang, Zhen Huang
BACKGROUND: Development of biofuels is considered as one of the important ways to replace conventional fossil energy and mitigate climate change. However, rapid increase of biofuel production could cause other environmental concerns in China such as water stress. This study is intended to evaluate the life-cycle water footprints (WF) of biofuels derived from several potential non-edible feedstocks including cassava, sweet sorghum, and Jatropha curcas in China. Different water footprint types including blue water, green water, and grey water are considered in this study...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28428819/lipid-production-and-molecular-dynamics-simulation-for-regulation-of-accd-gene-in-cyanobacteria-under-different-n-and-p-regimes
#17
Roshan Kumar, Koushik Biswas, Puneet Kumar Singh, Pankaj Kumar Singh, Sanniyasi Elumalai, Pratyoosh Shukla, Sunil Pabbi
BACKGROUND: Microalgae grown under different nutrient deficient conditions present a good source of natural lipids with applications for several types of biofuels. The expression of acetyl-CoA carboxylase gene can further provide an insight to the mechanisms leading to enhanced lipid production under such stresses. In this study, two nutrients viz. nitrogen and phosphorus were modulated to see its effect on lipid productivity in selected cyanobacteria and its correlation with Accase followed by molecular dynamics simulation...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28428810/recent-achievements-obtained-by-chloroplast-transformation
#18
REVIEW
Muhamed Adem, Dereje Beyene, Tileye Feyissa
Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the power and raw materials that can be used as sophisticated biological factories. They are rich in energy as they have lots of pigment-protein complexes capable of collecting sunlight, in sugar produced by photosynthesis and in minerals imported from the plant cell. Chloroplast genome transformation offers multiple advantages over nuclear genome which among others, include: integration of the transgene via homologus recombination that enables to eliminate gene silencing and position effect, higher level of transgene expression resulting into higher accumulations of foreign proteins, and significant reduction in environmental dispersion of the transgene due to maternal inheritance which helps to minimize the major critic of plant genetic engineering...
2017: Plant Methods
https://www.readbyqxmd.com/read/28427010/leveraging-microbial-biosynthetic-pathways-for-the-generation-of-drop-in-biofuels
#19
REVIEW
Amin Zargar, Constance B Bailey, Robert W Haushalter, Christopher B Eiben, Leonard Katz, Jay D Keasling
Advances in retooling microorganisms have enabled bioproduction of 'drop-in' biofuels, fuels that are compatible with existing spark-ignition, compression-ignition, and gas-turbine engines. As the majority of petroleum consumption in the United States consists of gasoline (47%), diesel fuel and heating oil (21%), and jet fuel (8%), 'drop-in' biofuels that replace these petrochemical sources are particularly attractive. In this review, we discuss the application of aldehyde decarbonylases to produce gasoline substitutes from fatty acid products, a recently crystallized reductase that could hydrogenate jet fuel precursors from terpene synthases, and the exquisite control of polyketide synthases to produce biofuels with desired physical properties (e...
April 17, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28421330/regulation-of-coniferaldehyde-5-hydroxylase-expression-to-modulate-cell-wall-lignin-structure-in-rice
#20
Yuri Takeda, Taichi Koshiba, Yuki Tobimatsu, Shiro Suzuki, Shinya Murakami, Masaomi Yamamura, Md Mahabubur Rahman, Toshiyuki Takano, Takefumi Hattori, Masahiro Sakamoto, Toshiaki Umezawa
Regulation of a gene encoding coniferaldehyde 5-hydroxylase leads to substantial alterations in lignin structure in rice cell walls, identifying a promising genetic engineering target for improving grass biomass utilization. The aromatic composition of lignin greatly affects utilization characteristics of lignocellulosic biomass and, therefore, has been one of the primary targets of cell wall engineering studies. Limited information is, however, available regarding lignin modifications in monocotyledonous grasses, despite the fact that grass lignocelluloses have a great potential for feedstocks of biofuel production and various biorefinery applications...
April 18, 2017: Planta
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