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Kiyoshi Saeki, Yoshihiro Miyasaka, Yoshihiro Ohishi, Takeo Yamamoto, Ryota Matsuda, Naoki Mochidome, Yasuhisa Mori, Kohei Nakata, Takao Ohtsuka, Kousei Ishigami, Yosuke Minoda, Yutaka Koga, Yoshinao Oda, Masafumi Nakamura
BACKGROUND: Intraductal tubulopapillary neoplasm (ITPN) is a rare pancreatic intraductal neoplasm. It is characterized by a tubulopapillary growth pattern, entirely high-grade atypical cells, minimal cytoplasmic mucin, and no obvious luminal mucin secretion. Most of its biological nature remains unclear. CASE PRESENTATION: We herein report a case of intrapancreatic recurrence of ITPN in the remnant pancreas of a patient who underwent pancreatoduodenectomy 16 years previously for a noninvasive intraductal pancreatic head tumor...
August 16, 2018: Surgical Case Reports
Eiji Noda, Kenji Kuroda, Tomohisa Sera, Takuya Mori, Haruhito Kinoshita, Tsuyoshi Hasegawa, Hitoshi Teraoka, Takaaki Chikugo
We report a case of intraductal papillary mucinous neoplasm arising within the heterotopic pancreatic tissue which was found incidentally in the jejunum during surgery for bowel obstruction. A 54-year-old female patient was admitted to our hospital due to sudden abdominal pain. In preoperative findings, we diagnosed bowel obstruction and performed surgery. Intra-operative findings showed adhesive intestinal obstruction, we performed synechiotomy for adhesion release. During surgery, when searching the small intestine, we coincidentally found a tumor in the jejunum and partial resected the jejunum...
August 2018: Journal of Surgical Case Reports
Lilli Saarinen, Pirjo Nummela, Hannele Leinonen, Annamari Heiskanen, Alexandra Thiel, Caj Haglund, Anna Lepistö, Tero Satomaa, Sampsa Hautaniemi, Ari Ristimäki
Pseudomyxoma peritonei (PMP) is a subtype of mucinous adenocarcinoma that most often originates from the appendix, and grows in the peritoneal cavity filling it with mucinous ascites. KRAS and GNAS mutations are frequently found in PMP, but other common driver mutations are infrequent. As altered glycosylation can promote carcinogenesis, we compared N-linked glycan profiles of PMP tissues to those of normal appendix. Glycan profiles of eight normal appendix samples and eight low-grade and eight high-grade PMP specimens were analyzed by mass spectrometry...
August 2, 2018: Molecular & Cellular Proteomics: MCP
Alicia M Barnett, Nicole C Roy, Adrian L Cookson, Warren C McNabb
The development and maturation of the neonatal intestine is generally influenced by diet and commensal bacteria, the composition of which, in turn, can be influenced by the diet. Colonisation of the neonatal intestine by probiotic Lactobacillus strains can strengthen, preserve, and improve barrier integrity, and adherence of probiotics to the intestinal epithelium can be influenced by the available carbon sources. The goal of the present study was to examine the role of probiotic lactobacilli strains alone or together with a carbohydrate fraction (CF) from caprine milk on barrier integrity of a co-culture model of the small intestinal epithelium...
July 23, 2018: Nutrients
Guilan Li, Shu Yang, Ping Shen, Bo Wu, Tingyi Sun, Haimei Sun, Fengqing Ji, Deshan Zhou
Mucinous colorectal adenocarcinoma (MCA) is characterized by a great mount of extracellular mucus fundamentally composed of Mucin2 (MUC2) which is significantly correlated with the high malignancy and strong invasive ability of MCA. However, rare is known about the underlying mechanism of the mucus accumulation in MCA. Our latest study demonstrated that SCF/c-KIT signaling was highly activated in MCA patients and mouse model, which up-regulated MUC2 transcription. In the present study, we paid a special interest in whether and how SCF/c-KIT signaling promoted mucus secretion by using wild-type (WT) C57BL mice and their littermates who harbor mutational c-kit gene (Wadsm/m ), clinical colorectal cancer (CRC) samples, as well as human CRC cell lines...
2018: American Journal of Cancer Research
Hyemi Song, Bong-Kwang Jung, Jaeeun Cho, Jong-Yil Chai
Gymnophalloides seoi worms were rapidly expelled from C57BL/6 mice within days 3-6 post-infection probably due to operation of mucosal innate immunity. To understand better the mucosal immunity related to worm expulsion from the host, we isolated exosomes of G. seoi metacercariae and investigated their role in induction of mRNA and protein expression of several Toll-like receptors and mucin-related factors in vitro. G. seoi-secreted exosomes were collected using differential ultracentrifugation, and cellular internalization of the exosomes into HT-29 intestinal epithelial cells was visualized by confocal microscopy...
July 17, 2018: Parasitology Research
Lingyu Yang, Chao Liu, Wenjing Zhao, Chuan He, Jinmei Ding, Ronghua Dai, Ke Xu, Lu Xiao, Lingxiao Luo, Shuyun Liu, Wei Li, He Meng
Establishing and maintaining beneficial interactions between the host and associated gut microbiota are pivotal requirements for host health. Autophagy is an important catabolic recycling pathway that degrades long-lived proteins and some organelles by lysosome to maintain cellular homeostasis. Although impaired autophagy is thought to be closely correlated with Crohn's disease (CD), the functional role of autophagy in the maintenance of gut microbiota is poorly understood. As autophagy-related 5 ( Atg5 ) is a key gene associated with the extension of the phagophoric membrane in autophagic vesicles, we established a gut-specific Atg5 knockout mouse model, and found that the disruption of autophagic flux in the intenstinal epithelium cells dramatically altered the composition of the gut microbiota and reduced alpha diversity...
July 13, 2018: Applied and Environmental Microbiology
Ipek Isik Gonul, Asli Cakir, Sinan Sozen
Background: Mucins may show aberrant expression, localization, and/or glycosylation in multiple malignancies. However, information regarding expression of these mucins is mostly unknown in urothelial tumors. Aim: This study was conducted for examining the expressions of membrane associated and secreted mucin (MUC1) and a secreted gel-forming mucin (MUC2) in urothelial tumors of the urinary bladder. Subjects and Methods: Archival transurethral resection materials of 97 urothelial carcinoma cases were reexamined light microscopically and graded according to the 2004 WHO Classification...
July 2018: Indian Journal of Pathology & Microbiology
Min Woo Kim, Seungho Choi, Sun Yeou Kim, Yeo Sung Yoon, Ju-Hee Kang, Seung Hyun Oh
Inflammatory bowel disease (IBD) is characterized by chronic or recurrent inflammation of the gastrointestinal tract. Even though the current strategies to treat IBD include anti-inflammatory drugs and immune modulators, these treatments have side-effects. New strategies are, therefore, required to overcome the limitations of the therapies. In this study, we investigated the anti-colitic effects of allyl isothiocyanate (AITC), which is an active ingredient present in Wasabia japonica . The DSS-induced colitis model in the mouse was used to mimic human IBD and we observed that AITC treatment ameliorated the severity of colitis...
July 12, 2018: International Journal of Molecular Sciences
Yuliang Jiang, Zhe Liu, Feng Xu, Xichen Dong, Yurong Cheng, Yizhang Hu, Tianbo Gao, Jian Liu, Lei Yang, Xingyuan Jia, Haili Qian, Tao Wen, Guangyu An
Aberrant O-glycosylation is frequently observed in colorectal cancer (CRC) patients, but it is unclear if it contributes intrinsically to tumorigenesis. Here, we investigated the biological consequences of aberrant O-glycosylation in CRC. We first detected the expression profile of Tn antigen in a serial of human CRC tissues and then explored the genetic and biosynthetic mechanisms. Moreover, we used a human CRC cell line (LS174T), which express Tn antigen, to assess whether aberrant O-glycosylation can directly promote oncogenic properties...
July 12, 2018: Journal of Cellular and Molecular Medicine
Je-Keun Rhee, Jinseon Yoo, Kyu Ryung Kim, Jeeyoon Kim, Yong-Jae Lee, Byoung Chul Cho, Tae-Min Kim
Mutation hotspots are either solitary amino acid residues or stretches of amino acids that show elevated mutation frequency in cancer-related genes, but their prevalence and biological relevance are not completely understood. Here, we developed a Smith-Waterman algorithm-based mutation hotspot discovery method, MutClustSW, to identify mutation hotspots of either single or clustered amino acid residues. We identified 181 missense mutation hotspots from COSMIC and TCGA mutation databases. In addition to 77 single amino acid residue hotspots (42...
March 16, 2018: IEEE/ACM Transactions on Computational Biology and Bioinformatics
Jiao Song, Qinghe Li, Peng Li, RanRan Liu, Huanxian Cui, Maiqing Zheng, Nadia Everaert, Guiping Zhao, Jie Wen
This study investigated the effects of inulin on mucosal morphology and immune function of specific pathogen-free (SPF) chickens. A total of 200 one-day-old White Leghorns SPF chickens were divided into 5 groups of 4 replicates of 10 chickens each. All SPF chickens were fed a basal diet supplemented with 0, 0.25, 0.5, 1.0, or 2.0% inulin. The mucosal morphology and immune indexes were analyzed on days 7, 14, and 21, respectively. Our results showed that the concentrations of acetate and propionate in the cecum and serum had increased with dietary inulin supplementation on day 21 (P < 0...
July 10, 2018: Poultry Science
Chuanjian Zhang, Yu Peng, Chunlong Mu, Weiyun Zhu
Background: Compared with oral antibiotics (primarily disrupt foregut microbiota), the present study used antibiotics with ileum terminal infusion to disrupt the hindgut microbiota, and investigated the changes in specific bacterial composition and immune indexes in the jejunum and colon, and serum of growing pigs. Twelve barrows (45 d of age, 12.08 ± 0.28 kg) fitted with a T-cannula at the terminal ileum, were randomly assigned to two groups and infused either saline without antibiotics (Control) or with antibiotics (Antibiotic) at the terminal ileum...
2018: Journal of Animal Science and Biotechnology
Samaneh K Sarvestani, Steven A Signs, Veronique Lefebvre, Stephen Mack, Ying Ni, Andrew Morton, Ernest R Chan, Xiaoxia Li, Paul Fox, Angela Ting, Matthew F Kalady, Michael Cruise, Jean Ashburn, Jennifer Stiene, Wei Lai, David Liska, Shao Xiang, Emina H Huang
Ulcerative colitis (UC) is a prevalent form of inflammatory bowel disease (IBD) whose pathogenic mechanisms remain unclear. Elucidating these mechanisms is important to reduce UC symptoms and to prevent UC progression into colitis-associated colon cancer (CAC). Our goal was to develop and validate faithful, human-derived, UC models and analyze them at histologic, transcriptomic and epigenetic levels to allow mechanistic studies of UC and CAC pathogenesis. We generated patient-derived primary-organoid cultures from UC and non-IBD colonic epithelium...
June 19, 2018: Oncotarget
Cong He, Yuki Fukumura, Akane Toriyama, Kanako Ogura, Noriko Sasahara, Keiko Mitani, Takashi Yao
Twenty-four surgically resected, gallbladder pyloric gland adenomas (GB-PGAs) were examined and their features were compared with the reported features of stomach, duodenum, and pancreatic PGAs to better understand GB-PGAs. Clinical information on background gallbladder lesions and histologic data, including tumor grade, existence of squamoid morules, intratumoral cholesterosis, and intracytoplasmic mucins were collected. Immunohistochemical staining for MUC2, MUC5AC, MUC6, CDX2, pepsinogen I, p53, and MIB-1/nuclear β-catenin were evaluated...
September 2018: American Journal of Surgical Pathology
Chao Li, Didi Zuo, Libin Yin, Yuyang Lin, Chenguang Li, Tao Liu, Lei Wang
Background: The reliability of MUC2 as a prognostic marker in colorectal cancer (CRC) is controversial. This study evaluated the association between MUC2 expression levels in CRC tissues and prognosis. Methods: The PubMed, Web of Science, Embase, Cochrane Library, China Biology Medicine disc (CBMdisc), Wanfang Database, and China National Knowledge Infrastructure (CNKI) databases were searched to identify studies exploring the relationship between MUC2 expression in CRC tissues and overall survival (OS)...
2018: Gastroenterology Research and Practice
Chihiro Minatsuki, Nobutake Yamamichi, Ken-Ichi Inada, Yu Takahashi, Kouhei Sakurai, Takeshi Shimamoto, Yosuke Tsuji, Kazuya Shiogama, Shinya Kodashima, Yoshiki Sakaguchi, Keiko Niimi, Satoshi Ono, Toru Niwa, Ken Ohata, Nobuyuki Matsuhashi, Masao Ichinose, Mitsuhiro Fujishiro, Yutaka Tsutsumi, Kazuhiko Koike
BACKGROUND: Sporadic nonampullary duodenal epithelial tumors (NADETs) are uncommon, and thus their clinicopathological features have not been fully assessed. AIMS: In this study, we have analyzed a series of early sporadic NADETs, focusing on various immunohistological features. METHODS: We conducted a multicenter retrospective analysis of 68 patients with endoscopically resected sporadic NADETs. Associations between immunohistological features and clinicopathological features were statistically analyzed...
June 28, 2018: Digestive Diseases and Sciences
Cuiping Liu, Chen Chen, Fuguo Yang, Xin Li, Lixue Cheng, Yang Song
Phytic acid (PA) has been demonstrated to have a potent anticarcinogenic activity against colorectal cancer (CRC). Defects of the intestinal mucosal barrier and inflammation processes are involved in the development and progression of CRC. In the present study, we evaluated the effect of PA on the intestinal mucosal barrier and proinflammatory cytokines. After a 1-week acclimatisation period, sixty Wistar male rats were divided into the following five groups, with twelve rats per group: the control group (CG), model group (MG), low-PA-dose group (0·25 g/kg per d), middle-PA-dose group (0·5 g/kg per d), and high-PA-dose group (1 g/kg per d)...
July 2018: British Journal of Nutrition
Che Wu, Zhenwei Yang, Cailiang Song, Chao Liang, Hongxin Li, Weiguo Chen, Wencheng Lin, Qingmei Xie
Yeast nucleotides are a fine functional additive in human and animals. The effects of dietary yeast nucleotides supplementation on intestinal development, expression of intestinal barrier-related genes, intestinal microbiota, and infectious bronchitis virus (IBV) antibody titer of specific pathogen-free (SPF) chickens were investigated. A total of 60 1-d-old chickens were divided into 4 groups, each of which included 3 replicates of 5 chickens. Group 1 served as a control that was fed a basal diet. Groups 2 to 4 were fed the basal diet supplemented with 0...
June 26, 2018: Poultry Science
Kentaro Kikuchi, Shuichi Fukunaga, Fumio Ide, Miyako Hoshino, Harumi Inoue, Yuji Miyazaki, Tie-Jun Li, Kaoru Kusama
Intestinal-type adenocarcinoma of the primary salivary glands is extremely rare. So far, only 11 cases of primary intestinal-type adenocarcinoma of the oral cavity and major salivary glands have been reported. Two of those tumors arose in the floor of mouth, 7 in the tongue, and 2 in the major salivary glands. However, it has remained unclear whether these tumors are derived from mature salivary glands, and primary intestinal-type adenocarcinoma of the buccal mucosa has not been reported previously. Here, we present the first documented case of primary intestinal-type adenocarcinoma arising in a minor salivary gland of the buccal mucosa...
May 30, 2018: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
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